日本血吸虫表皮抗原基因、其编码蛋白和应用的制作方法

专利名称：日本血吸虫表皮抗原基因、其编码蛋白和应用的制作方法
技术领域：
本发明涉及生物基因领域，具体地涉及一类日本血吸虫表皮抗原基因、其编码蛋白，还涉及该日本血吸虫表皮抗原蛋白的应用。
背景技术：
血吸虫病由血吸虫的感染引起，在世界上有76个国家，受感染者约1.5亿。致病的血吸虫有3种，既日本血吸虫(Schistosoma japonicum)，曼氏血吸虫(S.mansoni)和埃及血吸虫(S.haematobium)。在我国流行的是日本血吸虫。血吸虫病的流行给人民身体健康和经济发展都造成巨大的损失，因此，防治血吸虫病、开发研制治疗血吸虫病和抗血吸虫的药物具有巨大的社会和经济效益。
研制新的治疗血吸虫病和抗血吸虫的药物，首先必须找到合适的药物作用靶点。血吸虫的表皮是宿主和血吸虫相互作用的界面。而组成血吸虫表皮的蛋白质暴露在这个界面上，对小分子的化学药物和大分子的抗体药物而言都是最理想的药物作用靶点。已经有研究表明治疗血吸虫的化学药物——吡喹酮的作用靶点就是存在于血吸虫的表皮。另外一些已知的血吸虫表皮抗原蛋白，例如GST28，paramyosin，已经用于疫苗研究和应用。我们通过大规模的蛋白质组学研究，发现了50个日本血吸虫表皮抗原蛋白质，这些蛋白质，都是可能的药物作用靶点和候选疫苗。
鉴于日本血吸虫表皮抗原蛋白的重要功能，研究和开发日本血吸虫表皮抗原蛋白具有重要意义。

发明内容
本发明要解决的技术问题之一是提供一类日本血吸虫表皮抗原基因。
本发明要解决的技术问题之二是提供一类日本血吸虫表皮抗原蛋白。
本发明要解决的技术问题之三是提供上述日本血吸虫表皮抗原蛋白的应用。
为了解决上述技术问题，本发明通过如下技术方案实现在本发明一个方面，提供了一类日本血吸虫表皮抗原基因，其编码具有日本血吸虫表皮抗原蛋白质活性的核苷酸序列，所述的核苷酸序列包括(a)SEQ ID No.1～SEQ ID No.27所示的序列；
(b)SEQ ID No.1～SEQ ID No.27所示的序列中每条序列的互补序列；(c)与SEQ ID No.1～SEQ ID No.27所示的序列中每条序列有至少70％同源性的序列，及(d)上述(a)～(c)中一条或数条的组合。
较佳地，所述的核苷酸序列包括具有SEQ ID No.1～SEQ ID No.27所示的序列。
在本发明的另一方面，提供了上述日本血吸虫表皮抗原蛋白，包括(a)具有SEQ ID No.28～SEQ ID No.54所示氨基酸序列的蛋白；(b)由(a)的蛋白发生一个或几个氨基酸置换、缺失或插入而形成的具有(a)蛋白相同活性的蛋白。
较佳地，其具有SEQ ID No.28～SFQ ID No.54所示的氨基酸序列。
在本发明的另一方面，提供了一类能与上述日本血吸虫表皮抗原蛋白特异性结合的抗体。在本发明中，可以使用一系列本领域已知的方法来制备针对日本血吸虫表皮抗原蛋白的抗体。例如，将提纯的日本血吸虫表皮抗原蛋白产物或它的抗原片段注射入动物体内以产生多克隆抗体。同样，表达日本血吸虫表皮抗原蛋白或它的抗原片段的细胞也可以用来对动物致免疫而产生抗体。本发明制备的抗体也可以是单克隆抗体，这些单克隆抗体可用杂交瘤技术制备。
在本发明的另一方面，提供了上述日本血吸虫表皮抗原蛋白作为免疫原，在制备血吸虫疫苗中的应用。
在本发明的另一方面，提供了上述日本血吸虫表皮抗原蛋白在制备防治血吸虫病的药物中的应用。应用本发明的日本血吸虫表皮抗原蛋白，可研究血吸虫治疗药物的作用靶点，从而在开发研制预防或治疗血吸虫病的药物的过程中发挥重要作用。


图1是本发明日本血吸虫表皮抗原蛋白的免疫荧光抗体原位杂交实验结果示意图。其中，T代表tegument(表皮)；G代表gut(肠道)；ST代表subtegument(下表皮)，绿色荧光部分是Immunophilin的信号。
具体实施例方式
下面结合附图和实施例对本发明作进一步详细的说明。应理解，这些实施例仅用于说明本发明而不用于限制本发明的范围。下列实施例中未注明具体条件的实验方法，通常按照常规条件，例如Sambrook等人，分子克隆实验室手册(New YorkColdSpring Harbor Laboratory Press，1989)中所述的条件，或按照制造厂商所建议的条件。
实施例1 日本血吸虫表皮抗原蛋白的分离和鉴定实验1.日本血吸虫表皮的分离纯化血吸虫表皮的纯化按照Triton-x-100提取方法进行。表皮抗原蛋白从血吸虫雄虫、雌虫、雌雄混合成虫和童虫分离。虫体用Krebs’-Ringer’s，Tris-马来酸盐-缓冲盐液(KRTM)洗3次，再用KRTM/0.2％Triton X-100溶液悬浮。这个液体和虫体的混和物置于冰水浴中，在摇床上摇动10分钟。然后，装有液体和虫体的混和物的试管在涡旋混合器上混旋几次，然后再放回冰水浴中，静置使虫体下沉。用吸管吸出上部液体，放置到另一个新管中。这个试管在离心机上用2500×g的离心力，离心15分钟。得到的沉淀就是血吸虫的表皮。Krebs’-Ringer’s溶液的配方是0.15MNaCl，6.2mM KCl，1.2mM Na2HPO4，1.2mM MgSO4，10mM Glucose and 20mMTris-Hepes，pH 7.4。Tris maieate缓冲溶液的配方是0.2M Tris and 0.2MMaieicanhyclride，pH 7.4。
2.日本血吸虫表皮抗原蛋白的电泳分离日本血吸虫表皮用裂解剂裂解，蛋白溶液用SDS-聚丙烯酰胺凝胶电泳分离，条带切割后再做第二维的在线毛细液相色谱分离以及质谱鉴定。软件分析得到鉴定蛋白。分离好的血吸虫表皮，用SDS-聚丙烯酰胺凝胶电泳(SDS-PAGE)缓冲液悬浮，用超声波促进组织细胞裂解和蛋白溶解，超声波裂解方法为超声1秒，暂停3秒，总时间2分钟。并用100℃加热5分钟以使蛋白质变性。然后这些表皮抗原蛋白混合物用SDS-PAGE分离，分离方法按照普通的SDS凝胶电泳方法进行。胶上电泳泳道用刀切割下来，再用刀均等分割成8-12份。接下来做蛋白的胶内酶解。
3.日本血吸虫表皮抗原蛋白的胶内酶解过程胶内酶解按以下方法进行蛋白胶块用刀切碎，每个小块大小约为1立方毫米。加入100mM NH4HCO3，洗2次；转入到试管中；加入10mM二硫苏糖醇(dithiothreitol，DTT)/100mM NH4HCO3，56度放置反应1小时；冷却到室温，离心吸去DTT；加入等体积的55mM碘乙酰胺iodoacetamide/100mM NH4HCO3，室温/黑暗反应45分钟；吸去iodoacetamide，加入100mM NH4HCO3，洗2次；去掉NH4HCO3，加入乙腈缩胶；去掉乙腈，真空抽干；加入100mM NH4HCO3，胶膨胀，去掉NH4HCO3，加入乙腈缩胶；去掉乙腈，抽干；加入含Trypsin的消化缓冲液(50mM NH4HCO3/12.5ng/ul trypsin)冰上放置45min；去掉多余酶液，加入50mM NH4HCO3，以刚覆盖胶为准；37度，过夜酶切；酶切结束后，从试管中吸出上清液到新管子中，胶中加入60％ACN/0.5％HAC，在水浴中超声处理15min，再重复一次；吸出上清液到新管子中，在原管子中再加入60％ACN/0.5％HAC，37度40min，吸出上清液，与前面的上清液合并，真空抽气去ACN。
4.日本血吸虫表皮抗原蛋白酶解肽段的在线色谱分离和质谱鉴定酶解产生的肽混合物，用真空将溶液体积抽减到30μl。之后，每个组分的肽混合物上样到一个反相捕获柱(C18，5μm，300，300μm i.d×5mm，LC Packings)，在20μl/min的流速下脱盐。然后这个捕获柱和一个分析用的75μm×150mm C18柱(LC Packings)相连接，肽混合物就在200nl/min的流速下，从这个柱子上洗脱进入装有Protana NanoES的电喷雾离子源的QSTAR pulser i中。Agilent 1100毛细液相系统(Agilent Technologies)用来提供流动相A(0.5％乙酸/水)和流动相B(0.5％乙酸/乙腈)，线性梯度是60分钟内5％B到50％B，30分钟内50％B到90％B，然后是在90％B停留15分钟。在分析柱后通过一个不锈钢的两通(Valco Instrument)连接一个10-μm直径的PicoTip纳喷雾头(New Objective)，加上2500-3000伏的喷雾电压，以获得稳定的喷雾。MS/MS串联质谱谱图通过信息依赖的采集(IDA)和任务-循环增强来记录。在每个测定扫描中，选取3-6个信号最强、带2到3个电荷的离子，用滚动碰撞能量打碎，以获得串联质谱的信息。
5.日本血吸虫表皮抗原蛋白的软件搜索鉴定用牛胰酶的自裂解片段如([M+H]+)1153.5741，2163.0569和2274.1600作为内标，或者以预先数据库搜索得到的得分高的肽段的分子量，来校正所有的质谱谱图。质谱搜索用的数据库，包括从8420个从EST cluster预测得到的血吸虫蛋白质序列，以及可能的污染蛋白质序列如牛胰酶的序列、宿主蛋白污染如人蛋白质序列、小鼠蛋白质序列、大鼠蛋白质序列、兔子蛋白质序列和角蛋白序列等。搜索软件是MASCOT(http://www.matrixscience.com/，Matrix Science)。搜索参数为最大漏切点，3个；monoisotopic；肽电荷数，+1/+2/+3；修饰，半胱氨酸carbamoylmethylation和methionine氧化。搜索结果合并后，用下列标准进行过滤筛选1.对每一个串联质谱谱图的搜索结果列表，只提取排位在第一个的结果，其他的则丢弃；2.搜索得到的肽段中如果有KR，KK，RK，RR等序列，则这个肽段丢弃；3.搜索得到的肽段，长度在8个氨基酸以上的保留，小于8个氨基酸的丢弃；4.重复打到的肽段，只计算其中分值最高的，把这些肽段分值加起来，做为蛋白的得分；5.蛋白的得分超过60，就认为是可信的结果。低分的蛋白，手工检查后保留或者丢弃。匹配到宿主或者牛胰酶、角蛋白的肽段，则丢弃。
实施例2 日本血吸虫表皮抗原蛋白的生物信息学分析和功能注释1.同源性和相似性分析日本血吸虫表皮抗原蛋白序列对GenBank(http://www.ncbi.nlm.nih.gov/)的非冗余数据库(Non-redundant，NR)做BLASTP比对；日本血吸虫表皮抗原蛋白序列对Swissprot数据库做BLASTP同源性比对。根据比对结果得到的与已知其他物种或已知的血吸虫蛋白的序列相似性，做出日本血吸虫表皮抗原蛋白的功能注释。
2.文献查检根据已经发表的文献报道，发现鉴定得到的27个表皮抗原蛋白在虫体上的位置，有一些的确在血吸虫表皮上。而其他则是新发现的表皮抗原蛋白。举例说明，比如以下表皮分离和质谱鉴定得到的蛋白，根据文献报道，就是已知的表皮抗原蛋白，而且有些蛋白已经有进行了疫苗和抗原研究Paramyosin，tropomyosin，myosin，dynein light chain 1，22.6 kDa tegument membrane-associated antigen，tegumental antigen Sm20，nitric oxide synthase 1(NOS1)，leucineaminopeptidase，14-3-3 proteins，SnaK，cathepsin B-like cysteine proteaseprecursor，21.7 kDa antigen，FK506-binding protein 50(immunophilin)。
实施例3 日本血吸虫表皮抗原蛋白的免疫荧光抗体原位杂交实验为了进一步用实验的方法验证用蛋白质组学手段鉴定得到的血吸虫表皮抗原蛋白，选取了一个Immunophilin(SjFKBP50，GenBank登录号为AY815389)做免疫荧光抗体原位杂交做蛋白定位分析。
实验方法血吸虫成虫用冷冻包埋剂(OCT)完全包埋，切成5μm厚的切片。用抗Immunophilin的抗体覆盖切片，在37℃反应60分钟。该抗体是用重组表达的Immunophilin免疫小鼠制备的单克隆抗体。随后切片用PBS洗涤，再加入FITC-偶联的兔抗鼠免疫球蛋白的抗体(1∶40稀释，用含有0.5mg/ml的Evans蓝的PBS稀释)，置湿盒中37℃反应60分钟。染色结果用Leica DM-RB型荧光显微镜观察并拍照。
如图1所示，该实验结果证明了Immunophilin确实分布在血吸虫成虫的表皮上。这说明前面表皮分离和质谱鉴定的结果是可信的。
序列表<110>上海人类基因组研究中心<120>日本血吸虫表皮抗原基因、其编码蛋白和应用<130>NP-10280<160>54<210>1<211>1933<212>DNA<213>日本血吸虫(Schistosoma japonicum)<400>1ctggagtaca tgtttagact ctgatcatgc tctagtacga gcacgcattt gcttgcacct 60cactggacgt agaaaggcca cattaggaaa acccattaga attgatctga gcgtcaagaa120ggtcaaaagt aaatttcaag aacagttagg gtcacaatta ggtaactctg gaaaccaggc180tgatcctgat gttgcctgga atgacctaaa aacagctgta gaaacagcag cgtcatctac240tagtaatttg aacagaagga gcacgaaaaa ccagtggatt tctgcgacat ctatggcact300gatgagctct cgccaactaa tcccgtcagg ctctgaacac gatgaagaac gaaaacaaat360caagtctaaa ttaagtagaa gtttacgaaa cgatcgtgag cagtggtggg caacgaaagc420aaaagagatg gaaaaggcag cggctttagg caacactagg cagctttata gactaatcag480ggaaaccgga attaagaagt caaggataag tgaaactatc tccgagaaag atgatactgt540aatctgctct caacccagac gtttagaacg gtgggccgaa cacttcaagg aacagtttag600ctggccttca gctactctac aattacccag catttccact caagctaaat ggaacattga660agtaggcccc ccgccactag ctgaagttca aaaagctata tctaagctga aacgaggaag720agcagctggt tcagatgggc taaccccaga ggttttcaaa gatggtggtc cagtcttggc780gattaggttg actagtattt tagctaaagt ctgggaccta gataaaattc catccgactg840gtctaaatca ctaatagtcc caatatataa gaaaggacca aagtcatctt gtgataacca900tagagggatt agtttgacta atatagcatc taaaatacta gcctcaataa ttatcggacg960tttaacaaag actcgtgaac tacaaacacg agaaaaccag gctggcttca gacctggtcg 1020tggttgcatc gaccacatat tcaccattcg tcaggtttta gaacacaggc acgcctaccg 1080gcgtcctaca atggtggttt tcctcgactt aaaggcagca ttcgactcag ttgaccgtga 1140ggttctgtgg cagtgtctgt cattaaaagg tgtacctcag aagtacataa accttgtgaa 1200gctctttact cgaacactac caggtcgagt gacagcttat ggcgaactgt catcggaatt 1260cctcacttca agtggtgtcc gtcaggaatg tccactttcc ccatttttgt ttaattttgt 1320catagacatg ctcctagaca taaccttatc ttcgtctgac ttttcaggag ttgactttct 1380accaggagcc tcgcttactg acttagagta tgctgatgac atagtcctat ttggtgaaga 1440cgctgacaaa atgcagagtc ttctgactac cctgagcaac aatgcaagca tgttcgggat 1500gcggttttcc ccctcgaaat gcaaaatgtt gcttcaggat tgagttgcat cggcacctga 1560cctagtgata gggagtgaag tagtcgagcg tgtagaccgc ttcacgtatc tggggagtct 1620catcagtgct gatggcctgg tgactgacga aatctcagca cggatacaga aagctcgatt 1680ggcttttgcc aagttgcgtc acttatggcg cagacgagat atccgcttac taaccaaagg 1740
acgagtgtac cgtgcagcag ttcgctatgt cctactatac gggaacgaaa catgggccgt 1800taagagtaga ggacattcgt aaggttactg gtcttccacc ctaggtgtct ctgaaacgtt 1860gctcgtggat cgtgagacac ccaagtgagc aatgctgtcg ttaggaaacg ggttctagga 1920aaggaggcca tca 1933<210>2<211>2160<212>DNA<213>日本血吸虫(Schistosoma japonicum)<400>2gcttaatatg gtttatctga cactgtcctt aagctctatt tgcaaagcta ctagactaaa 60ctgtctatcc aatttgcacc aatctttaaa atgtcgaaaa ctaagttttt caagcggata120cactgttatt gaccactcat ttgatgcggt agtcgttggt gcaggtggag caggtctacg180tgcaggtttc ggcctagcaa atgaaggatt caaaactgct attataacaa aattattccc240taccaggtct cacactgttg ctgcccaagg tgggataaat gctgctctcg gtaatatgga300ggccgatgac tggcgtttcc atatgtatga cacagttaaa ggctccgatt ggttgggtga360tcaggatgct attcagtata tgtgcgagga ggctccaaaa gccgtcatcg aattggaaaa420ctatggagta ccttttagtc gtttggaaaa tggtaaaata taccagcgag cattcggtgg480tcaaagcata gattatggac gtggtggtca ggctcatagg tgttgtgcag ttgctgatcg540cactggacat tctcttctac atacattata tggtcggtca ttacgttatg atgctaccta600tttcatcgaa tatttcgtat tggatctatt aatggaaaat ggtgagtgtc ggggtgtcat660tgccgtgtgc ttagaggatg gaactctcat cgtttccgtt ctagaaatac aattcttgcc720acagggggtt atggccgtac ctacttttct tgtacttcag cacatacttg cacaggtgat780ggaacggcaa tggtcacacg ggctgggttg cccaatcagg atatggagtt tgttcaattt840catcctacag gaatttatgg tccgcggctt gatcactgag ggctgtcgag gtgagggcgg900ttacctcatc aacagtaaag gagagagatt tatggaaaga tacgctccta atgctaaaga960cctagcttct cgcgacgtag tttcaagggc gatgacaatt gagatccgcg aaggacgggg 1020agttggccct agaaaagacc atatattttt gaaactttct catcttcctg ctgaacagct 1080tttcagtcga ctccctggca tatcagaaac agcaaaaata tttgctggtg ttgatgttac 1140aagagatcct attcctgtat tgcctaccgt ccactacaac atgggaggaa ttcccactaa 1200ttacaaagga caggttttga catatgatcc tgtcgctaaa aaggagaaag ttgttccagg 1260attgtatgcc gctggtgaag ctgcttctgc gtcagttcat ggtgctaatc ggttaggagc 1320caattcatta cttgatatag ttgtatttgg ccgtgcatgt gctctggata ttgctgctaa 1380acataaacct ggagacgcag gtccggaatt aaaagcagat actggagcgg cctctatcgc 1440taactatgaa aaacttcgca cggcgaatgg aaaatattca gttgctcaag ttcgcttaga 1500aatgcagcga acaatgcagg agtatgcggc agtattccgt gatggtccta cactcaaaga 1560aggctgcaga aaaatgtatg agttatatac ttctcgaatg aatgatttga aagttagtga 1620tcgtagcaaa atatggaata gtgatctgat ggaggcatta gaattacaga atttgatgct 1680aaatgctttg cagactattg ttggagcaga ggctcgtaaa gagtcacgtg gtgctcatgc 1740acgtgaagat tttcctaatc ggctagatga attcgactac tctaaaccta ttgatggaca 1800aaaagccaaa ccttttgaag aacattggcg aaagcatact ctatcctatc aggatgttaa 1860taccggtgct gtgaaattgg aatatcggcc tgtaattgat gccactttgg attctaaaag 1920ttgtccaact gttcctccga aaatgcgcac ttattagatg aatttttcat gtgtagctga 1980
atcaactgaa gatctgtttc cactcgattt attcaggatt actttaaata atcacttcga 2040caatacattt ttactaatct actatatgtt attgattttt tgtgaatgtt tttgttcaaa 2100caacagtcgc aataaaatat cttctttaaa aaaaaaaaaa aaaaaaaaaa aaaaaaaaaa 2160<210>3<211>1526<212>DNA<213>日本血吸虫(Schistosoma japonicum)<400>3tgacacttta tttgacacct attcgcatgt catatctatt attttttaat cattaagata 60aatacattca ccacattgac ttgaaatcat ggtactgaag ctcggtccag ataatcctcc120gattgttatt gacaacggaa caggtttgtt aaaagttggt tatgccggtg aaatggaacc180tcgtgcagtc gttcctatga tacttggtcg accaaaacac atgaaacttc ttgttgacaa240ggattctgat gaatgcttcg taggtgatat tgccggtggt aaaagaggtg cattaacttt300atcttatcca atcacgaatg ggattgtaaa taattgggat gacttagaaa ctatctggca360acatgttttt actaatgaat tacgtattct tcctgagaat caccctatgc ttttgacaga420aacaccactg aatccgaagg taaatcgtga gaaaatggcc gaaattatgt ttgaacattt480ccatgcacca gcggtgtaca tctcaattca agctgttctt tcactctatg cttcaggtcg540cacaactggt ttagtcttag actgcgggga aggtgttaca catttagtcc ctgtttttga600tgcctatgcc atcccaacgg ccatacatag aagaaatcta gctggacgag aggtaactaa660ctatctgaaa tacttgctca ctgaaagagg ttataagtta atcacaacat gcgaacatga720aatggcccgt gatgtgaaaa aaaatgttgg ttatgtccta tggactacgg agaaaggact780acaacttagt cgaggagata agaaatcatt tgaaacgaca tatcgtttac cagatggtgg840cttgttaaat attggcaatg aaaggtttcg tgcacctgaa ttaatgttga atccgaagct900ggctggttat gaaatgaatg gtatccacag tgctacatat gaaactatac tagcttgtgg960catggatgtt agaaaaagct tttggtcaaa tgttattctt actggtggag gtacaatgtt 1020ccgtggtttc ccagaacgac ttcgtaaaga actgaatcgg ttagctccaa gaggtgcaac 1080agtaaaggtg ttagcgtctc ctgaacgtaa atactctgtt tggattggtg gtgccgtact 1140agcttctttg agtacgttta atgaaatgtg ggttaccaaa gaagaatatg aagaattcgg 1200ttcagaaatc ctacatcaga aatgcattta actacatcga aatactctga tatttaattc 1260aggcagttgg tgtgcaacag aagttaaagg tagtttcatg cagtccccgt ttgcaaattt 1320acgtattatt aaactttata tatataatat gattgatata aaatccttca catttctaca 1380tatttgtagt ttattgacat tttggcaaat attacgccac ccattgaatc attatgttac 1440tttatagatg tttatttctt caaaatagtt tatttgtttg aataattaat acatattcaa 1500tataattgaa aaaaaaaaaa aaaaaa1526<210>4<211>1106<212>DNA<213>日本血吸虫(Schistosoma japonicum)<400>4gatcttcctt atgttagtct aacaaataag tagctatcgt atctacggtt aatgtgtggt 60
ttacaagcgc tagaggatat aaaaaagttg gctgaagatg attcaaggta taccgccctg120atgaagcctc tatcccagga caagactaca ctacagggac ttgtattatt actttcaaac180aaaaacaagt ctattgtttc actagtgctc caaatcttga ttatcatacg tcgtcggcca240ggtggtgatg acgttttgcg atcattattg ggactaagtg aacaactaaa cgatctgaaa300aaatctacta actctattga caacgaagat aaaaggctta tttctaatag cgctgaagaa360ctcctatctt tatttaactg ctccactaag cagaattctc ccaaacctat aaaagctaat420ccagagaaag cacacttcat atctaggccg aaaagtgtaa ttttacagct cagagggcta480actagtgagg tgatatcgaa ataatatgtt caaaaactct tgaaaattcg cggtgtcgtt540agtattacat ttcaattgca cagacatcgt gttcttgttt gcacaatacc ctcgttagat600cctgattgtc ttatacaagc aatatcttca gttaacaata attcagatca gtcagatggc660atacatcgaa caaattcaaa tcaccctaca atcacagccc aaattgtcaa gaaaaaacat720cgagtacaat ctttatgcag acacagttca ttcaatggtt ttaaatatag acaaagcttg780aaaaatactg aagtcccacc ctatctggaa gatgatgctg atctattcga ggtcgatgaa840acaagggccg ctccaaaact tagagatgct ccaagcaaac aagattcttc taagggtcca900atcggttggc tgtcagattt cctagaaaag tcactcttct ggtgaacttt agatacattt960ttttaccttt acaggtttta cagcaataag tagatatcta ctgcatttag aagcagaaat 1020acaaccaaac tttttatgtc ttatttttat aacttgtgtt tcttgcaata aatctacaat 1080atatctgaaa aaaaaaaaaa aaaaaa1106<210>5<211>1127<212>DNA<213>日本血吸虫(Schistosoma japonicum)<400>5cttgtttccc cgtagaggaa agcggcacat tacgccacta cccctattaa ttgggggtat 60atctcccctc cttagggccc cccgcagggc cttacaactt ttaactcttc ccggcttaat120ttgtttgcgg ggcattttgg agcggataca cattttaccc cagggaacca ggttatgccc180atgtttccgc ccagccttaa aattaacccc ccttaaaggg gaccaaaacc gggactttcc240ccgggggggg cgcccccctt tagaatctgg gggtttcccc cggggcgcca ggaatttggc300cgggaggctt tttcaagttt atagaaaaaa tagtttgtca tttagctgct ttttcattat360gaagaagaga ccaaagctta ctcttatttt cgatgaggag aaacgaaaag aatatcttac420tgggtttcgg aaaagaaagc tcgaacgtaa agaaaagggt cgtaaagcat cagaaaaact480attgaaagag gaaattaagg ctgttaagga aaagtacaga gaggctgccc gtaaaaagct540tgaaaacatt cagttgcctg ggatgctgga ttctgttgaa aacatccttt ccactgaaaa600acacattgtt ggagatcagg acgttgttat tcaagaacta gatttatgta gttcacatta660ctttatgggc tcttgtaagg aacaaaatcc taatccaatc attcatagtg aagagccata720tatgtcactt aaaaaagcac tcaaaatgaa tccagtgaga agcaaaaagc ggtttcgtca780tagaaagaaa agacactttt caaagcataa acgtcaaaac tagtcatttc atcttgtata840ttttcttatg ctaaataatc taatggttac ttctctacga aagcacaaca tacaacttcg900gggggggccc cggtacccca atgtccccta aaatgggccg ttattacatt tcctggccga960tcattttaca acgctcctga ctggagaaaa cccggtgggt gtacccaaac ctaacgcgcg 1020ttgcgggaac ttcctacttt tatcccgttg ggggttaatt gccgaaagag gtccgcttcg 1080aatcgtgcct ttctcaaaag attggccaac tcctgaatag gcatagg 1127
<210>6<211>2000<212>DNA<213>日本血吸虫(Schistosoma japonicum)<400>6cggagtagat ggagaggatg gtccagatgg cagcccagga gatgatggcc cacccggtcc 60tccaggtcca ttcggcgagc ctggtccagc tggtgaacag ggaccacaag gtccacctgg120gaacaaggga gccatgggac tgactggccc aaggggtgaa actggccaaa gaggtgcacc180tgggccaact ggtgcaacag gtgatgttgg ggaacctggt ttggccggac ctccaggaat240taacggaaat gtaggaagga aaggaccaac ggggcaacgc ggacaacctg ggccaagagg300caagcaagga gacgctgggg aacctggaaa acccggagag gccggcccac atggttatcc360tggattcatg gggcctccag gagaaccagg cccagagggc cctgctggta gcgaaggaag420cgaaggccca ccaggggaac agggacctcc tggaaagaat ggtgaacttg gtgaggctgg480tgatgtaggt gacgcaggac cgccaggacg agctggacca cctggtcgtc gaggtcatcc540cggatttccg ggtgctcgag gaccacctgg agaggtaggg aaacctgggg aaccaggacc600tgtcggaccc atcgggcgcg ctggaatgag aggacctcga ggatcggttg gtggtgtcgg660agttgcagga agtaagggtg aacaaggact ttctggagct ccagggtctc ctggagagat720aggaccgaga ggagattctg gagagccggg cattcctgga aaggacggtc gcccaggtaa780acaaggagaa ccaggcccca aaggagcacc tggaggtaaa ggacctgcag gacctcccgg840gcctcctggt ttagatggcc ccgtggggta tcctggagat caaggtccaa gaggccctcc900tggccaagta ggggagagag gtccgatggg ctctagaggc tcgcgaggtg atagaggtga960tcctggtgag gttggaccac ttggtcctcc tggaagggat ggcgaattgg gacctcctgg 1020tccgcaagga ccatctgggc cccctggatt gcctggccca cctggacaaa ttgtttctat 1080gcaggctaga gcgtcaagaa ctaaaggaat aatgtacggc gatgatcctt caattaaaag 1140gagatttgga aacatcgaaa caactcttcc ccaaggaaca ccagaattcc ctgctcgaac 1200atgtgcacat ttggcggata tacatcctaa taaacctgat ggtgaatatt ggattaaccc 1260tattggcgat ataattagca gtccgattaa agttaattgc cgtatgaaaa ctaaggaaac 1320atgtattaaa cctaaacaaa ttaagatctc aatggataaa ataccgtatg ttatgaatgg 1380tagattttgg ctccaagaaa taagtggagt caatgagtta aattatgcta ttgattcaga 1440acaactgagt ttcctgaaac tatcaagtga tgtagctatt cagcgtatat ctttatcatg 1500ttctggatca tcggacttgg tagttaattc tactatatct ttggctgaaa atattcgtct 1560actaggtgat gatgatacag ttttggaaag atatcatcca cttagaaact tcctcgttga 1620agaagatcaa tgtggaaaaa ctaccaatgg ttatacatct gtattgatta atggacatcc 1680aagtaaacta cctattcgtg acgtcaattt gttacattta aatacgttca actatgaatt 1740aattcttggc gatgtttgtt acacaacata tttaggaaaa gttgtttctt taaagcatta 1800tttaaataac taaacaattt taacgggtta acataagtaa taataaaacg aaaagaaaga 1860tttatcattg aagtagatgt tattataatt actaataaaa aactcatatt cactgagtag 1920tgctttctgt tatactaaaa cgaaattctg tacacaaatc aaaaaactaa ataaatgttt 1980atgatagtat caaaaaaaaa 2000<210>7<211>2114<212>DNA
<213>日本血吸虫(Schistosoma japonicum)<400>7gtcattcgtc atcttagttt ttattaggaa aagtgaagtt agggggggtt aaatgtacaa 60ttctaaagtg gaattaaatc tagtgtaaat gttggctcaa ggtatatttg cgcacatctg120ctgtcggctt ttacggcgga atttgggatc acaggcggtg aaaccttttt tttatcaaga180tatattgcag ttggataaac ctccgtatgc agagacacca tttcgaaagc taacgggtga240tttcgtttct ctcaaaaaaa ttgatgacaa agaaattttg aaaattgatt ctaaggcact300gattttgttg agtcagcaag ccatgataga tgtcgctcat cttttaagac ctgcacacct360acaatctctt tctaacatcc tgagggatcc tgaagcatct aacaacgata agtttgttgc420acttgagctt ttgaaaaatg caaatgtggc tgctggaatg gttctacctg gttgtcagga480tactggcacc gctatatgca tggcgaaaaa gggccagttt gtctggacag atgggaatga540cgctgaagct atctcgcaag gtatatataa cacatatact tctagttatc ttcgttactc600ccaagttgcg gcacttgata tgtttacgga aaaaaatact ggtactaacc tgccagcaca660aattgaaata tattcaactg gtggtaacga atatagcttt ttgtttattg ctaagggtgg720agggtcagcg aataaaactt tcctgtatca acagacaaaa gctcttctca atcccactag780tctatattca tttgttaatg aaaaaattaa aaccttagga actgcagctt gtccccctta840tcacttggct gttgtcgttg gtgggacgtc agctgaattt actctgaaaa ctgtgaagct900tgcatcatgc aagtatttgg atcaccttcc atcgactggt aatgaacatg gaagagcttt960tcgtgaccgg gaagctgaag aaaaaatatt acatttaact cagaaggttg ggatcggagc 1020acagttcgga ggaaaatact tctgtcatga tgtacgagta atccgcttgc ctaggcatgg 1080tgcatcatgt cctgttggta ttggtgtctc ttgttctgca gacagacaga ttctggggag 1140aataactaac gatggagttt ttcttgaaca gttagagact gatccatctc aatatttacc 1200tgacccttct ttaaatcaac tcccaacaga tgttgtcaaa gtgaatctca atcttccaat 1260gaaagaaatt ctagctgaac tttctaagta tccaatcaaa actcgtttgt cactgactgg 1320aactttagtc gttgcacgtg atatcgcaca tgcaaagatt aaggaacgtc ttgattctgg 1380tcaaccaatg cctgactatt tttgtaatca cccggtatat tacgctggcc cagctaaaac 1440acctatgaat tatgctagtg gatcttttgg acctaccacg gctggtcgca tggattctta 1500tgttggtcaa ttccagcagg ctgggggtag tttgattatg ctagctaaag gtaacagatc 1560aaaagctgta acaaatgctt gcagaaaaca tggtggattc tatctaggtt cgatcggcgg 1620tccagctgca atccttgctc agaattgtat aaagaaagtt gaagtactcg agtatccgga 1680attaggaatg gaggctatct ggcgtgttga agttgaggat tttcctgctt ttatagttgt 1740cgatgataaa ggcaatgact tctttaagga atggcaagta gaataaatac ccaaatatag 1800tgaatcactt acaaaatatt cctttttttt aaatttaatt agttgccttg tcgatgtttg 1860atcactggtt ctgttttcgt tgaccatttt tttgtttatt aaaacttgtg ggtccgttta 1920cctgtgaata caagttggtt gtaatttata gttactcagg tattatttta atgtcagata 1980cgtaacactt atcattcagc ttaatgattg taaacttctc tgtcttcgtt tctgaaattt 2040cgattaattt ttttgttaaa ataaatccac ttatcttcat taagtatata tcacaaaatt 2100tcataaaaaa aaaa 2114<210>8<211>1501<212>DNA<213>日本血吸虫(Schistosoma japonicum)
<400>8gttctaagag gcttgtcctt tagtgatcaa taagaattca gttaaaactt tggcagcatg 60gtggctctcc aatgagtcat ctcttaatta cgtggggcta acgccaaaaa gcgggcgtgg120gaaagtgtac attgacatgc atagtccggg gattatcgtt gggatgccgt ttgttgaagc180cattctaaag gaaagtagtt gcgagattga atggcatatt tgcgagggtc aaccagttaa240atcatgtcct gttagagtcg ctacaatcac tggggctaac gaagacatat tcttctgtga300aaacctggtt ctttcagtac tttcaagagc cagcggtatc gcatccctag ctagcaggat360ccaatcaatt ttgcaggaag tttcatggaa gggaacaatg tatatgcctg acagaaggac420accaggattc ggtcttgttg aggaatatgc tatgatgata tctggaattt ctgagcggaa480gtcatctgtt agcgttcgtt gcaaagatat ggatgctgga agcctaaaat ctgctatcga540cgaagttcgg tctagagttg gctccattcc tgtccatgtt gcctgttgta ggttggatga600agcctgttta gctgctggag caggggcaga tatattactc actggtttaa gtgcgaagga660aattgtagac attgcttcac aagtaaaaga attcttatcc agaaatccaa gtttattgcc720agcggctctt tttgatgatt cggacgtaaa gctgtgtgca acccgctatg ttgatcattt780gacatctcta aaactgtgca atgggtatgc gtttggtgat ttccagatga cgtatgtaag840gatagtgcta gaataccgaa actacaaaat atgtccttct ggaaaaaaat ggtctcatga900ttttatttcc agccacaaag atcaaatcct cctcgatcgc aagcacctag tggtcccctt960tcttcaacat gtcaaatccg cgacttatgc caccaacatc cacattacga gagccccaac 1020tatgctctta caaccaggtt taaatatcaa taatcaagtg tcaggtgtgc tgattccacc 1080aaataatcaa cagaatgtca atatgcgttt acctatgata cctgctcaat tacagcagcc 1140accacaacaa atgccacaac gtatgccatc aaactctccg acagtactag gtacgaatca 1200gccaatgggt ggtggatttt taaatagcat tcatcctcct cataatcagc ctccaaattg 1260gcaagtgatc ccaagcggtc ccagtggaca tccaatgggc gggattggtt tagggatgcg 1320tgttagtggt cctggagggc caaatatgaa taactgccga ttatgtggtt ttccgaatcc 1380ttcatcaatg cctttttgtc gaaattgtcg ttctggcctt cgttaggtct aaaggttctt 1440tgtatgcttt gtgcttaatt tattataatt tatgttcact taaaaaaaaa aaaaaaaaaa 1500a 1501<210>9<211>1983<212>DNA<213>日本血吸虫(Schistosoma japonicum)<400>9atcaaattta cttgcgagat gattattaaa gagtttcgca taattcttcc cttaacggtg 60gatgaatacc gtgttggaca gttgtgggct gttgcagaag catctaagaa tgaaacaggt120ggtggtgaag gaatagaagt tttagtaaat gaaccatttg atcgcgaagt caatcctccg180gaatcacccc tgattgcaaa tggtgaagaa tttttttctg gacaatacac ccacaaacga240ttttacttgg ctagtaaagt tcctggttat gtacgctttc ttgctccaaa agggtcacta300gaagttgatg aaaaagcatg gaacgcttat ccctattgtc gtacaatttt acagaatccc360aaatacatgc aagataactt tactttaatt attgagtcta tgcacgtaca agataaagtt420aatatcgaaa atattcacaa ccttccacca aaacttttgg ctcaacgaga agttatatac480attgatattg caaacgatca attgcatatc gcctcagatt atgatccatc atgtgatcct540aggatatatc aatcagtgaa aactggccga gggccattag taggtccaag atggtgggaa600
aatacagacc taccaataat gtgtgcgtac aaactagtca catgtgaatt taagtggtgg660ggtattcaag gacgggtgga aaatatgata caagtcaaga aagacggatt tttcttaact720ttcaatcgtc aggtcttttg ttggctagat aaatggcatg gactttctat gagtgatata780cgggatgttg aaaataaaac taaaaggaat tggaccaaca acgtcttatc ggtggtattc840gcggcactgt ggctcatgat taaaatgatg tcatcattaa aattgacaat tttgctgttc900taaacagatt acaaacgtat taatatacat attcgcaaat ttgaagtaac caaatgcttc960tttaatcaaa ctcttgcgtg tattgacaaa taaatccatc ttgaaaacca tgtcacacag 1020cctagtattt tacccacctt tccatcgaat cacgtcttct ctgatgggca agagtgagga 1080agtggcgaat aaactgtcac tatctacagt ttcgtttgca aattctttcc ttctgttctt 1140tcgtccattt tgtaatatgt gtgatgttct gtcttggttt taattattcc caatgacaat 1200tgtcagtcac tttctttata catatacgta tgtattgact aattgcgcca aattttaagc 1260tacgttttta caaaataatc taaaatttta tttttcagtc agttatttct gtttccgatt 1320tctatccttc cttgttattt atttgtttat tgttcatagt ttaattaatt cgactgttaa 1380tctttacttg cgatgattcg aaaattaata ctgttgttgt cattgaccag tcaactaata 1440cttacagcat taagcatttt ggaattttca ttaaacacat tccgaaaatt tgagaaagaa 1500tacctgtaca catatttttt atatttatat cctccctcac tttttattat cgttttgccc 1560gattttgttt tttacctgaa actaagaatt ccaccattta tgtgtttatt gttattatac 1620attactgttt tactgaataa ccaaaaaatg acaagttacc tgattaggtc ttttttgtca 1680ctttttattt tgctattatt gtcatcgtca tcgctgccgt catctttgtt gtcattataa 1740atagcctata aagaaagtag gcaaacagaa tttttccctt tgctttaata tttagtcgaa 1800tattacatcc ctggattgca tcaaccttca ttttaccttt gtctacttac tatatttgtt 1860tattcatcct gtgttttagt ttatttgtta attggtctgt atttaactta ttaggtgaat 1920taatatatga tggcaatgaa gtatttgtga taaaagtatt agtaccgata aaaaaaaaaa 1980aaa 1983<210>10<211>927<212>DNA<213>日本血吸虫(Schistosoma japonicum)<400>10ttttctacac gtcgaacagt cttctgtcca taatacgtgc acatgaagct caagatgctg 60ggtatcgtaa tgtatcgaaa aaatcaagcc actaatttcc cttcacttat aaccatattt120agtgccccta attatctcga tgtgtataac aataaagctg ctatacttaa atatgaaaac180aatacaatga atatcagaca atttaattgt tcacctcatc cttattggct tccgaatttt240atggatgtat ttacttggtc gttaccattc gttggtgaaa aagtcacaga aatgcttatg300aatgtattaa gtatatgttc tgatgatgaa ttgatgaagg aaggtgacga tacatttgaa360ggtggaactg tagctgctcg taaagaagtg attcgtaata aaatacgtgc aattggcaaa420atggctcgta ctttcacagt gttaagagag gaaagtgaat cggtatttga actaaaaggt480ttaacaccga atggaatgtt gccattaggt gctttgtctg gaggaaagga atcattaaat540aaagcacttt ctggtgtttc acctagaaat aaaatcatat cttttgaaca agcaaaagca600tatgaccaga tcaatgaacg tatgcctcca agacgagata gttcagcttt agctacaact660cctacttctg ttcgaacatt tggttctgca tcccgtcaaa gtgataaaaa accagcgcct720aatccacctg gtgttgctgc tgtcaatgct gtggactcaa actatcggcg acttagcgca780
aaaagcaata cgagtcaagc agtcaccaat gaaaatgata tacatcgttc ttcaagtact840agtggtatcc ggtcatgtag taatggtgag actggaatta attccttgac aaagtcatct900gatcccacgt cgacgcggcc gcgaatt927<210>11<211>1368<212>DNA<213>日本血吸虫(Schistosoma japonicum)<400>11taaactatag aaaagttaaa tacatgtttg gcaagttgtc agagggcaca atctattgct 60gaatcaatgg cagaagacca taaaagagct ttataggaag aaataaagac taaaactatg120ttccaaagta aattgaatac aatgcaaaaa gaattagaag tatcagttaa tagagcggac180gaagaagctg aaaatgtaga aaagcttcaa attcagctca ctaaattcac tagcgaatta240gcacaaacca aatcaaaata tgaaaaagtg ttcagtgaga aaactgaaga atttgatgag300ctcaaacgtc gtttgaacaa tcgtatcaat gaattaacag aatcgtatga acttgaacgt360ggtcgtgtag ttagtctaga acgcaccaaa aaccaattgt ctcatcagtg tcaggaaatg420caatctcaac tagataattg tgtggggaat gcacagagca agctgcaaac tatcaagagt480ttagaaagtc aaaagacgga acttgaagca gttttggaag aagcgctagt agaagaaagc540aatttgcgaa caaaatgtgg tttgttacag agggatctga ttcaaatcag agctacaaag600tcagaaatgg aggaacgcgt gacagttttg gagaaagaga acaagcaaaa ttccgaaaaa660ctgaaggaaa ccaaagaacg tttgtcagta gtcaacagac aagttaccga tttggagagc720tctcgggcaa gattagaagc agaaagagat aatctggaca ccacattaag ggataccgaa780gatgcactac atgaatgtga agctcgttac caaacaacat tttcagcatt gagtaccctg840agaaatgaat ttgaacgtca gtcaagagat aaggaagaag aattagaaag tttaagacgt900tcaagccaac gtaatgtgga gaatctcaaa gcagccataa cagatatgga aatgaaaaat960caaaatgagt tagaccgact taagaaaaaa ttagaaggaa atataaacaa tcttcaagtt 1020cagttggagg aggagaaaaa tgctcatttg gaaactatta aagtacatga agaaacagaa 1080gcgcatattt gtcagttaga aatgaatata acagaattga acaatctagt ctctgaaact 1140gcaaacactt tacagatatg tgaaagtcgt cgttctgctt tggcaaatga agttgagggc 1200ttacgttgtc aatttgggaa atcacagaaa aattggaaac ggaaactgga agaagagtca 1260tgtggaaaat atgtcaaagt tcggtggcct gaactttaca gtaataatct gacatagaaa 1320gacgaaactg gaaggtcaag tgggccgggc tacaaggtga tttggatg1368<210>12<211>1800<212>DNA<213>日本血吸虫(Schistosoma japonicum)<400>12tcttcaagag cagctaaaaa acaagagctt attgaaaatc tgaagcgtct ttttcctgga 60gttcatggtc gattgttgga aatgtgtcag ccatcacatc gtcgttatca ggtggcgatt120acgaaagttc ttggaaaata tatggatgct atagtctgtg atagcgagaa aacggctaaa180gaatgcattc agtatatgaa agatcaacgt attgaaccgg agacgttttt accacttgac240
tttttagatg tgaagccaat cgacgaaaaa ttacgtgaaa tcagtgatcc acctaacgtt300catttagtta ttgatgttat ccaatgtgat ccaatcatag taaagaaggc actaacattt360gcatgtggta atgcattgta tgtgaaactg ttgaacatgc tcgttatgtt gcctatcata420tgggagatag aaaaaaagac tgtatcatta gaaggcactc tgtttcaacg ttcaggtgtt480atatctggtg gtgcaagcga tttaaaagct cgagctcgtc gttgggatga aaagcaaatt540agtacactta tgtcgaaccg tgacgcctta caaaatgaat taaaagaaca attgaaacgt600aagcgtaaag aagctgaatt aagaactatt caatcgcaaa tcaaaggttt agatacacga660ttaaagtata cattaaaaga taaagatagc acggaagaga aacttcttag tacaaacgaa720gaggaaatga atcaaattgc tcgtgaatta gaagaagttg aagaaagttt aggtcgttgt780cagacgaaaa tgcaggaatt acaaatatca gttaatgcag agaaagccaa aatggatact840gtggaagata ctgtgtttca tgatttctgt gcacaaatag gggtagaaaa tattagacag900tatgaagacc gagagttaag agttgcccgt gaacgtgatc gtaaacgact tgagtttaca960aatcagcttc aaagaataaa taatcagttg gaatatgaac gatcacgcga tactgaagca 1020aacgttaaac gttgggaaga gactgttgct gtagaacgaa ctgaaatgga taaatgtaga 1080aacaagaaaa aaagatataa ggaagaaatg gagcaggaag aaaataaaaa aacggagatt 1140gaatcacgtg ttggggaatt aaaatatcgt gcagaaatgt tagatggaga attaggtgaa 1200attcgtagac gtctagtgaa taaacaacgt gatatacaaa aactgcaaaa agatttgaat 1260caggctgaag cgaaattaga atccagacga gccgaacgtc attcgctttt acaagcagct 1320aagatggaag atttagaact gccactgaaa ccaggttgcg atccaattcc tgaattgagt 1380agtcagttga cagaaagtga aaatatagat cctagtaccg aagaaatggc acatatttac 1440gaattggaag ctcgcctgcc aattgatttc aagcatcttg ataaaccttt acgacaaatg 1500aatgatgaga aagaagttaa tcgaaaagct gaagaaatgc aaaaccaagt cgattcaatg 1560ttaaatagtt tggcgcgcat acaagctcca aatttacgtg caggtgataa actaggtagc 1620gttgaagagc gtctacgatc aacagaagct gaatttgaag atactcgacg tagagctaaa 1680cgtgctaaag ctagatttga acgtgttcga cgattacgat ataatgcatt tatgaattgc 1740ttcaattcca ttgcggataa tattgatccg atttataaaa gtttgtcaag gaatcctggt 1800<210>13<211>1173<212>DNA<213>日本血吸虫(Schistosoma japonicum)<400>13catgggttta ttaaagcaat catgtgagta cttattccct ggcttccatt tcgtcaatcg 60cttctgtatc tactaataaa tctttggttt tagtaaataa ctcgacaatg aagtctccta120agcttactgc cacaccggat tccaaaagta agcagttgag taatggtaag acaactccag180cgaggccaac atctaagcgt ccaatccctg tcggtagcga tgacagcgat gcagaaactg240aatcaaatgt attgtgcaac aatgagctga agacgtctca caagaaaagc cattctaaag300tttccttctc tggaggcccg aaaggatgct gtttcaaagc cgaagttttt ttccggtcaa360aaattaaaac ttgcgctagt aggtggacca gaactcaaaa ccgctgtcgt ctggggtttt420ccggccgatg caaagaaatc agacataaaa gagttgttca acggagtagt ctcagtttcg480cgtgaaactt ccaatttcgg aaacaagcaa ccacccatgg ttgttgagtt tgagaccgct540gcggactgcc tcaatgcaac cacttcaaac gcaactttgg aaggtcgacg tttgacagtt600cttcttaaag aagtttactt ggataatctt aaccaaccaa agcaatcagg tcaacttgtc660
aataaaacta aagtcgcaga aacaaccgat agttcaaaag ccaaagtttc aaaggcagag720gaggttgctg atactgacga tgatgaaggt gatgaagatg acgatgatga agatatgagt780gaagaggatg acgaaactga tgacgacgac gagagtgaag aaaatagtag tgaagaatgt840tttcattgat caggagcgaa agtcagagtt tcgaagacga tcagataccg tcgtagttct900gaccataaac gatgccaact gacgatccgc gttggttcta taattgacat cgcgggcagt960ccccgggaaa cctttaagtc tttgggctcc ggggggagta tggttgcaaa ttcatttcgc 1020ggtcgtggtc gtggtggtca gtcgccccgt ggtaggggtg gtcagtcttc acgtggtcgt 1080ggtggtgacc gtggaggttg gtcgaaacgc ggtggccaca aaaacggcta agtagctctg 1140tacattcaga ttacaaataa acaattgtga atg1173<210>14<211>2073<212>DNA<213>日本血吸虫(Schistosoma japonicum)<400>14tttgtgtcac tgtaggttta ctgtgtaagc attttgatgc ggacgtattt cgatcaagca 60aaaacgcaaa tattcgcgat caagtatttg agacacttat gtttttcaca gaacaagtta120aatcagacat tgagatgcgt aaaaaagcat tatctggttt aggttttctg tgtactcgtc180atcatgagct actttgtggt cctcgtcttt gtaaattcta tcatgatctt ttgcaagctc240cttgtgatga ggcgaaatct aaatcaactg atcatcattt ggaattaaag tgtattgtgt300tggaaaactt gttaaacttt tttcttgagg aagaaaaacg aatgttggaa gctgatgcga360aatggaaggc tagtcaacac caagaatcgt taaaagaaat gggcgatata gcttcaggta420tggggagctc agttgcgcag atgtatctca aagacatact ggagtcattc ttttcaccat480tctttactgt gagactcact gccctttctg tcataactac aattcgtcgt caaggtcttg540ttcatccagt ccaaactgta ccttacttga tagctatgcg atctgatatt gatccaaata600ttctgattaa gctgatgctc acttcaggaa tagataccaa attcccaggt tttctgacga660tgcgagctgc tcagggtgtc aacttatctt atcgtcttca actcgttttg tatgacgaga720tacgtgaaaa gctaaaagta aattccggag ggcattgttt ccaacaatta ggttcgcctg780aaccttcaaa tattcctaag tccacgtatt ccattactgc tacacgtcga aatattcgca840gcagagctga tgctgatagt ttaagcttcg atgataatgc aaaatgctcg ccaactacaa900atgtctctga atctcagtcc atttccaatg ttccagaaga ttcttatggc gctattcgcg960gtactagaga tcctttcagt gaaattcctt cggctttgaa ttcgcactta tattcaatgc 1020ttagaaacaa taaaagtcaa cgccgttctc tgcttaacgg cctcatatca ctttttgatg 1080actcacagaa aattccactc agtcagttgg tgtatgtcgc tgataatttg gcccattttc 1140cataccagtc acaagaagag cccttatttg tagttcatca tatagacttg atggttagta 1200tggctggttc aggagttttg aacagtgttc gagaggcact tttcccagag ctaaaagcag 1260ctctagaggc catagttgct gctcaacttg aagcggatgc aaaaaatcgt gcacaaatag 1320agttacaatt acacgcagag cttcaggcac aacaagttgc atcagttgat gccactaata 1380ggggggatca tgagacagct ggattatttg ctgatcagat tcagcacact attcaaagaa 1440taaacagttt gaattcctca aatcctatta tgaggagtaa ttcatctata tctgatttct 1500ctgcaggtgt tattcctgga atagaaggtg tttcccgtgt tgatctgggt cctttcgaag 1560tagaagagga ggaagatccc atagcattac ttgatagatt gtctaagtct cgtcagacat 1620ccttaccaat agttcgcgac gcaattcgta caagtcgtgc ttgtgttttg ttgcttacac 1680
ttaaacattt tctaaaagaa tgttacagta tcactgatag caaaattcaa cgttactcgc 1740cttccgattc agcaaaatta tgggaaaagc cgttaactcg acgtgtgggt attcattttc 1800atccaatacc ttgtctaaaa gttgctggga atggaatcca taaatcagat tctcatgccc 1860gctacagttc tcaatcatct attaagacag atcataaatt cgtagattca tctaatcccc 1920catatcacaa agtggatgtg aaagagagtg cattcatgaa atgaaatctc tcatccgagg 1980atacttagac ttccgaaact aatctcacta tcgatcctcc agagaagagg aggtagacct 2040gatgactgaa tcctcatcca gctggtgggg gcg2073<210>15<211>1214<212>DNA<213>日本血吸虫(Schistosoma japonicum)<400>15caagagttat tattcaacgt accatttact ggaagtgtta aattgatgag tattattata 60tctggtgttg ataatgaaaa ccatccaagt caagtatcat tgtataaaaa caaaccttat120atgacctttg aagatttaga cgctgaatgt gaccaatcac ttgaactaac tattgatcca180aatggtgagg tgatatatcc attgaaaatt gctcgatttt ctaatgttca gactcttagt240ttacatatca gtgcaaatta cggtggtgat acaacacgaa ttcattatat tggcttgcga300ggagattata caccagcacc tagacgagaa gttgttatta caaattatga agtaacccca360aatatcgctg atttgaagga tactgtatta caaactcaga gtcgttttgt tgagtagtaa420aaataaaaat atttcgttat gcgatttttc ttcctacctt aatagttcgt atgcattgct480tattcctgaa cgttattttt ttagaaagta taaatggcgt gataaggaca atgaattgca540gcagtaatct agttcggaat atctctcaac ttctttcacc tttaattacc gaggacgaat600taaaacatct ggaaaaactt ccaagagaag ctttatctag taaatcatcc caccaagatg660ttactgcttt ttgtctgtta aaaaatgcag aagatgaagt tctacgttta tttcataaaa720aagaatactt cagaaaagct tataacgata cacttgaacg aatgaatact ttggctaata780gtctaccatc ctatgctctt gattacatta caacaccggg atggcatgaa aacttacata840ataaagacga tgatcatgat gaggaggaag atattccact agatcctgat tatgttaatt900ttatgaagaa aactatactt catcgtcaaa ttcgtgataa caacaatgaa tcaacttcaa960tagacataca atcaatgctg aaagcaccac ctcgtaaaat aaacaaatct acagatattc 1020aaacaatcga tgctcaaatt gaaatgcttg aagcttcctt attacattat tatgaatttg 1080tatcaccttg tcgtgattca ccagtatggc ctattttacc gttaaaacga actttgtaat 1140cattagtagt ttgttaattt cacgccaaca cttattatat aaatgtaatt gaattttgga 1200aaaaaaaaaa aaaa 1214<210>16<211>427<212>DNA<213>日本血吸虫(Schistosoma japonicum)<400>16ccaaatatac tggagcatga ctgatagcgt gaatgagaat tgcgtattct ttaaaaaacg 60cagtcgaaaa aatgtgcgac agaaagaagg ttcttctagt gaagatgaaa cagttattaa120
aactcaaaaa agacgtcata ttaataatat gctatatcaa cagacttcaa agtttggtgg180tagcggaaag tacttttctg atgatgatga tggtaataat aatgacaagc ttgatagtgg240cgcccttgaa accgtcactt acaaagcttc acattccaag gataacaaaa gaatatattg300cgacagccac tttagagatt gatacggatg taaagtgtga tgcacaggct atatttgaaa360atgctcagaa aattaaccag aaatctgaaa atcgtaatgt ctatatggga ctgaacaatt420acgccca 427<210>17<211>732<212>DNA<213>日本血吸虫(Schistosoma japonicum)<400>17acagcctaat attgaacaag gtaaattaca aatgtgggtt gatatatttc caacatcttt 60gggtgaacct ggtccaccgt ttgatatttc accaagagaa gcaaatgaat atatattgcg120tcttgttgta tggaatacat tcgatgttgt actagatgag aaatcgataa ccggtgaaaa180aatgtctgat atttatgtta aaggatggtt agctggttta gacgatcgtc aaaaaactga240tgttcattat agatcactta atggtgaggg aaatttcaat tggcgtttcg tgttcccatt300cttttactta cctgccgaaa atacaatagt tgttaaacgt aaagaacact tttggtctat360ggatatcacg gaacaacgtg ttcgtccaca gttagtatta caagtatggg ataatgattt420attttcaccg gatgacttta ttggcacatt agaattgaat ttatccaata tgcctagtcc480atcaaaatca cgtagtaaat gcgtgatgaa tatgctacag tcgattggca atgaaaccaa540aatggttaat ttatttgaat gtcgacggct gaatggattt ttggccattt gtaaacgagg600aaagcggaca ccactcttaa ctggtaagat ttgaatggaa atggagttgt taacaaaagc660agaagctgat cttcgaccag ctggtaaagc acgtgaagat ccaaatgata atcccattta720gaccaccaaa gc732<210>18<211>709<212>DNA<213>日本血吸虫(Schistosoma japonicum)<400>18tggtcgggct tgcctatcgt gatgacgcaa tcgggctata ctggctggga caagcgttcc 60ttaaggttct accatgccag gcaggtcggt tgaagagcgg taagactaaa agcagcaaag120ttgaattgcg agagcaaagt tgtactgccg actgtacaga agtgtgatgg aggttaccag180cggttgttga gaacgatttt atcaaatctt atccattact tgaaccaatt tcactatgca240aacgttacca atttgcacac ggatggatta cgcgtatctg ttatttgacc agaagacatg300ttaaggagac attccttgtc aaccttatga ttaaattgtt aacccatcag catattagac360aatgtgattg acgtaggata ttggataaaa tattcaataa gaatggaaat gtcaaagaaa420ctggattcag tgaatgcacg aatcaatgaa tgaatgaatc atctttgaat aattgaaggc480aatgaattat cgtatttgat tatgaagaac actgtgatgg aagtgattca actgattgat540tatgtaatta attaaataat tggattcatt aatcctgtgt acagaaattg ggtggaaaaa600aacaactaat taacttattc ttataaaggt ttatttcgga caaacaaaca acaaaaatac660
aaaaactcga agttttaaaa taaatatatt ttgagggttc cttacaaca709<210>19<211>704<212>DNA<213>日本血吸虫(Schistosoma japonicum)<400>19aacaacaagc caaacaattg ctattgcaca aatttgtaaa ggattgggta ctttgattgg 60atcatttctt tctggcctca ttaaagatta tacagaagat tatcgtcctg cttttatttt120tcttggggta atgcaattca ttggtttcat cacatcgttt acttccatta tatgggccag180aataaattca caaaaacata aaaatcacga tgttgttgaa aatctaatat caagtagtaa240tatgaatatt tcggaaattt cattatccca atcagagtta aatgaagatg taatctcaaa300tgataataaa caacctattt cttcttcttc gtctcctatt cataatgaga ataataataa360atatattcgt caaaatccta cagtttaatg ccttaatgca atttcttaat ccatgcatga420ttcatcttca ttatttccac ttatatattg aacagataga ctattcattt atcaatcttt480catatacata taaacactcc ataaccgttc ttcaacagaa ttttttatta accttggaga540ttatacaaga tgttctttta ttactaaagg ctgtttctag ataatttact catctatctt600cttatccatt agctcctgta tttcattatg cccggttaat tttgactgat tgtactatat660atttatatct cttcatttat taataaatca gctaaaaaaa catc 704<210>20<211>714<212>DNA<213>日本血吸虫(Schistosoma japonicum)<400>20cgtactgtat gtacttatag gaacaaaact gagttttata ttaggaagaa taaaagctaa 60ccccctctcc ccaccagaat aacaaataca gttgtttgaa ccaacgatct atgtttaaat120atgatcgttt atgctaaagg tcgagtttgc attgcatcga taagagcaat tctagtaaca180gattgctaaa ctagaaccgt cagaggttag aatgttcaac gactctgtat tcggctaaaa240ccgcttccta aggatttaag acatccaatc gctgagattc tgacatgtgt tgtggtgatg300aaaattcagt gtcatcatct ggaagtacca gctttggtga cgctgacacg tattatgtac360atcatcacga cggctcccat atattgtatg aagtctttat tagttgcatt tctatccgaa420atgtatcgcc cactgtgtat ctattttgtt ctcatatcaa aattgacatg ttgattaaga480gagccttcct tcatttatta attcgaaata ttattgcttc taattgtgta gtgtacatgt540ttgcagtatc catttgtatc aaattgtacg tgttttaata aataatcatt acatatacaa600tttcacacta aattattcac atttacacac tttgaaaatc aagttcaaca caaaacatga660ttgaccaacc tagttagtca taggaaatat agtgttcttt ctaatgactt gatg 714<210>21<211>823<212>DNA<213>日本血吸虫(Schistosoma japonicum)
<400>21ttattccagc tgtaacatgg gctccgccca tagttgccaa attgccggtt ggacgtgtcc 60cattttatac attcgtgcaa ctactgggtg ccctcctcgg gtcggttggt cgtcttcgca120gtgtatcgtg agaagattct agaatatgct agattacatg atcatgataa gcttctattg180attccaacag gtggcatatt tgtcacaaat ccaagtgcct ctcatttaac atgttttctt240gatcagattc tcggcactgc attattgacg gctggagcat tcgccattat cgatcgaaat300ggatggaatc ttcctgacca cttacatcca atacatctag catttctagt ttatgcttta360gttggatgtt ttgcgttgaa tgctggagcc gctttgaacc ccgctcgaga tttaggacca420agactgatga tatttctttt cggttggggt tcacaagcat tttctggtgc aaattatttc480ttttggatac ccatagttgg cccatatatt ggtgcaatca ttggaggagt tctatatgaa540ttaactatcg gcatacactt agataggaaa tcagattttg ttgtagatgt ggattatgat600gaaacacgaa gtgatggcaa taagttgatg gcttattcat caccataatt ctctcaaaac660tatcgatgaa tattctacta tttgtcaatc atcctccctg tttaacaatt ttaatgaaca720aggcattata tttctacgta gatcacgtga tggtaaccgg ttaccaggtg ttttgttttg780aatagttcaa attttcatta gacaaaataa taaattcatt gac 823<210>22<211>733<212>DNA<213>日本血吸虫(Schistosoma japonicum)<400>22tgtaaatata cactgtccta ttaataagag agaaatataa tagtgtgtaa aaatcaatca 60aaacaattag ataggttgaa atgatttgat ggatcacata ataaaatcat tcaaatggct120tagcaacttg acttcagata ttttgattcg taaacaatta tgcatagatt tttttagtga180cagaatagca cttgagaatg cctttatcat attacagaat accagtagaa tcacaagtaa240cttttgatcg aaagcactgt tcttaaattt ctgttaaaat aagtccgaaa tcaattcatt300cccactatac agagacaata gcacttaaca tgttattgat agtttttttt tggttagcgt360ttttcttagc aagcttgttt tttacaggat agagctgttc accccattcc tgactcactt420cctttgttcg agctaagaaa tggcagtaac cttaagtgga ttccagacgg agtccatatt480tgatgcagat gtgaaggcat tgattgatat ggcaagggca gcatttcgac agttgaaaaa540tatctgggac ttcaaccacc tgtcagtcaa catcaaagtc agcattttca atacaaacgt600gaaaacacta caattgtatg gagctgaaac ttgcatgaaa aagtgcaaaa agcactattc660aaaaaataca agtatttatg aactgttgtt tgaatgagat acttctgatc cgttgactag720acaccatcag cag 733<210>23<211>378<212>DNA<213>日本血吸虫(Schistosoma japonicum)<400>23cagatatctt actcatttag acataattca ctggtgttgt ttcatcaatg agaatggtct 60gtcacatcgc actgttgtta tcagttgtga tgattgtttc atttgatgca tcagtgagtg120
gtgagtacaa tttgacgaaa gacgatgtcg gtccattttt attgggtgta gcaatgggag180aagcatcctc tgatgaatcg tcaccagttg aaactagagg cagtaaaaag aagaagaaaa240acaagaagaa tggcaataaa tcatcgaacg attcaaaaga aaagaataaa gctaatgctg300ctacgtttcc aaggacaact tattccagtt aaaccttctt tagtatgcta aattaataaa360ttattgttta ttatacta 378<210>24<211>1135<212>DNA<213>日本血吸虫(Schistosoma japonicum)<400>24ccaaaggaaa acatgtttac ctgtgaagtc atttcaccct gttaataagg ccatttttag 60actaaattat tttttaatta gaaaatcaaa ggataatacc ccaagaatga atggattcca120aagtacttaa gaattattaa caatataacc agtactttac ccacattttc attataaaag180aacatgaaag cgaaggatat tgagtttgag atagtgatga atgtgtagca cacagagtaa240ttgtatcatt tgtattgata atagtagaaa tccgaatgca gaaaaaagat tgaagtctac300ttgggcgcct ataattccat tttatatgta tatgagtaaa atttttagta aaaatgaaaa360aggcagatca aataaaaaaa ggattggtga aagaacgagg gagtatgtat tgaatgtgct420aggacatagt atatattgtc gatttctggt aaataataca atataattga gattgttgca480tactttgaat aaataattgc ataaataaat agttacataa taaattacgt gtgttgttta540aaaagaagat tgaaaaacag tgaaaatttg aaaataaaaa tcattaaaaa aaattcaaga600gaatgtacct gtttcttcga tcttgttgta ctcattcaca atactagttc catattcctg660gacatgatgc atattaattg aatattaaat tgtgattaca aaataaagca gttgtaataa720taataattgt gaaaaacttc ctattcatac acgttttttt aaatcaacct gagctatgaa780atatccgcat gaggcacaca gagattcttg cctaaaacaa tatttacact cagagacaga840gatggtaagc aacataaatt ccgaaatctt catgaaataa caatactaat acagaagaca900gtacaaatgt agatttgaga ttagatttaa ataattctac acccacacgt atcccacaca960gataaagaaa ggcaataatg tataaataac tactagacta gtttaacatc agaagaatgg 1020ggccacacag acactcattt gtattggtga tattatggat cgtagggggg gggagcaagt 1080aacgaaagcc ctttaggcat cgctaaatga taaactgtaa taaagcctac aacaa1135<210>25<211>696<212>DNA<213>日本血吸虫(Schistosoma japonicum)<400>25ccctgccagg gcattgtgtg cgcactgctg gagagcttca tattaggacg aaatagctgc 60ttatgccctt ctagttttta atgataacga accaaaataa ctctgtaaca agcacaaaca120acgagagttt atgagagttg tttgcataca gctatgcaat taatacgttt acacattgat180cgagaatgca tacggactaa ttctaaaccc aatcgcttag aatttttcgt caggtagtcg240ctaataggtc tcgataatat gcgtgaaagt cgaaaaacta tgatttgtcg taagaatgat300taactgtaaa accaacacaa tattgcctta tactcaatat atatgtcgga aatgaattta360
aggaagacaa gaatcgatta aaaaaggacg ggaaaccaac tgtctcacca gtttgcaatc420gactgaagct gcaaatgcaa ttaattagat acctactcag tagtttaagg ttatgggaag480aaatgaaggt cttcggagtg ggatcgcaaa tgtgcaccaa gttacatgtt aaacaaatca540cggctttggt tggtttcaga tagaaacctc tcaaaatttg caccaatctc tgtaaaattt600gctatggtaa agcatattct tcgaaacagt tacacaccca gtatttctta ccttggttcc660aagcaatata tctgtttgtt caggtacgtt actctt 696<210>26<211>300<212>DNA<213>日本血吸虫(Schistosoma japonicum)<400>26agaaattggc tgttattgca cacttggaga aaacaacagg agaaaatctt gcaaattcag 60cactttttaa tcctaaagaa gaagtaaccg ctcgtagcag ctgttcagct ggagatacta120aacaaactaa aaacgatgac attaaagtcg aacagtctaa ttctaacaca gcggctcgta180ttgtattcag acgacgacct gcatctaatc ggagtatacg tacgtcacag gacgatgagt240aacaattcct tgcgacagtt atgtacatac ggtattttaa tgaatattat aatcactttc300<210>27<211>572<212>DNA<213>日本血吸虫(Schistosoma japonicum)<400>27ctaccttgac gtggtggtcg ggcttgccta tcgtgatgac gcaatcgggc tatactggct 60gggacaagcg ttccttaagg ttctaccatg ccaggcaggt cggttgaaga gcggtaagac120taaaagcagc aaatttgaat tgcgagagca aagttgtact gccgactgta cagaagtgtg180atggaggtta ccagcggttg ttgagaacga ttttatcaaa tcttatccat cacttgaacc240aatttcacta tgcaaacgtt accaatttgc atacggatgg attatgcgta tctgttattt300gaccagaaga catgttaagg agacattcct tgtcaacctt atggttatat tgttaaccca360tcagcatatt agacaacatg aatgagtaac cactaaccat ccatcacgtg gcgtattagt420tcgtgctatt ttaacatgat gaatgtgatt gacgtaggat attggataaa atattcaata480agaatggaaa tgtcaaagaa actggattct gtgaatgcac gaatcatgaa tgaatgaatc540atctttgaat aattgaaggc aatgaattat cg 572<210>28<211>513<212>PRT<213>日本血吸虫(Schistosoma japonicum)<400>28WSTCLDSDHALVRARICLHLTGRRKATLGKPIRIDLSVKKVKSKFQEQLGSQLGNSGNQA60DPDVAWNDLKTAVETAASSTSNLNRRSTKNQWISATSMALMSSRQLIPSGSEHDEERKQI120
KSKLSRSLRNDREQWWATKAKEMEKAAALGNTRQLYRLIRETGIKKSRISETISEKDDTV180ICSQPRRLERWAEHFKEQFSWPSATLQLPSISTQAKWNIEVGPPPLAEVQKAISKLKRGR240AAGSDGLTPEVFKDGGPVLAIRLTSILAKVWDLDKIPSDWSKSLIVPIYKKGPKSSCDNH300RGISLTNIASKILASIIIGRLTKTRELQTRENQAGFRPGRGCIDHIFTIRQVLEHRHAYR360RPTMVVFLDLKAAFDSVDREVLWQCLSLKGVPQKYINLVKLFTRTLPGRVTAYGELSSEF420LTSSGVRQECPLSPFLFNFVIDMLLDITLSSSDFSGVDFLPGASLTDLEYADDIVLFGED480ADKMQSLLTTLSNNASMFGMRFSPSKCKMLLQD 513<210>29<211>649<212>PRT<213>日本血吸虫(Schistosoma japonicum)<400>29MVYLTLSLSSICKATRLNCLSNLHQSLKCRKLSFSSGYTVIDHSFDAVVVGAGGAGLRAG60FGLANEGFKTAIITKLFPTRSHTVAAQGGINAALGNMEADDWRFHMYDTVKGSDWLGDQD120AIQYMCEEAPKAVIELENYGVPFSRLENGKIYQRAFGGQSIDYGRGGQAHRCCAVADRTG180HSLLHTLYGRSLRYDATYFIEYFVLDLLMENGECRGVIAVCLEDGTLIVSVLEIQFLPQG240VMAVPTFLVLQHILAQVMERQWSHGLGCPIRIWSLFNFILQEFMVRGLITEGCRGEGGYL300INSKGERFMERYAPNAKDLASRDVVSRAMTIEIREGRGVGPRKDHIFLKLSHLPAEQLFS360RLPGISETAKIFAGVDVTRDPIPVLPTVHYNMGGIPTNYKGQVLTYDPVAKKEKVVPGLY420AAGEAASASVHGANRLGANSLLDIVVFGRACALDIAAKHKPGDAGPELKADTGAASIANY480EKLRTANGKYSVAQVRLEMQRTMQEYAAVFRDGPTLKEGCRKMYELYTSRMNDLKVSDRS540KIWNSDLMEALELQNLMLNALQTIVGAEARKESRGAHAREDFPNRLDEFDYSKPIDGQKA600KPFEEHWRKHTLSYQDVNTGAVKLEYRPVIDATLDSKSCPTVPPKMRTY 649<210>30<211>380<212>PRT<213>日本血吸虫(Schistosoma japonicum)<400>30MVLKLGPDNPPIVIDNGTGLLKVGYAGEMEPRAVVPMILGRPKHMKLLVDKDSDECFVGD60IAGGKRGALTLSYPITNGIVNNWDDLETIWQHVFTNELRILPENHPMLLTETPLNPKVNR120EKMAEIMFEHFHAPAVYISIQAVLSLYASGRTTGLVLDCGEGVTHLVPVFDAYAIPTAIH180RRNLAGREVTNYLKYLLTERGYKLITTCEHEMARDVKKNVGYVLWTTEKGLQLSRGDKKS240FETTYRLPDGGLLNIGNERFRAPELMLNPKLAGYEMNGIHSATYETILACGMDVRKSFWS300NVILTGGGTMFRGFPERLRKELNRLAPRGATVKVLASPERKYSVWIGGAVLASLSTFNEM360WVTKEEYEEFGSEILHQKCI380<210>31<211>150<212>PRT<213>日本血吸虫(Schistosoma japonicum)
<400>31MCGLQALEDIKKLAEDDSRYTALMKPLSQDKTTLQGLVLLLSNKNKSIVSLVLQILIIIR60RRPGGDDVLRSLLGLSEQLNDLKKSTNSIDNEDKRLISNSAEELLSLFNCSTKQNSPKPI120KANPEKAHFISRPKSVILQLRGLTSEVISK 150<210>32<211>273<212>PRT<213>日本血吸虫(Schistosoma japonicum)<400>32LFPRRGKRHITPLPLLIGGISPLLRAPRRALQLLTLPGLICLRGILERIHILPQGTRLCP60CFRPALKLTPLKGDQNRDFPRGGRPPLESGGFPRGARNLAGRLFQVYRKNSLSFSCFFIM120KKRPKLTLIFDEEKRKEYLTGFRKRKLERKEKGRKASEKLLKEEIKAVKEKYREAARKKL180ENIQLPGMLDSVENILSTEKHIVGDQDVVIQELDLCSSHYFMGSCKEQNPNPIIHSEEPY240MSLKKALKMNPVRSKKRFRHRKKRHFSKHKRQN 273<210>33<211>603<212>PRT<213>日本血吸虫(Schistosoma japonicum)<400>33GVDGEDGPDGSPGDDGPPGPPGPFGEPGPAGEQGPQGPPGNKGAMGLTGPRGETGQRGAP60GPTGATGDVGEPGLAGPPGINGNVGRKGPTGQRGQPGPRGKQGDAGEPGKPGEAGPHGYP120GFMGPPGEPGPEGPAGSEGSEGPPGEQGPPGKNGELGEAGDVGDAGPPGRAGPPGRRGHP180GFPGARGPPGEVGKPGEPGPVGPIGRAGMRGPRGSVGGVGVAGSKGEQGLSGAPGSPGEI240GPRGDSGEPGIPGKDGRPGKQGEPGPKGAPGGKGPAGPPGPPGLDGPVGYPGDQGPRGPP300GQVGERGPMGSRGSRGDRGDPGEVGPLGPPGRDGELGPPGPQGPSGPPGLPGPPGQIVSM360QARASRTKGIMYGDDPSIKRRFGNIETTLPQGTPEFPARTCAHLADIHPNKPDGEYWINP420IGDIISSPIKVNCRMKTKETCIKPKQIKISMDKIPYVMNGRFWLQEISGVNELNYAIDSE480QLSFLKLSSDVAIQRISLSCSGSSDLVVNSTISLAENIRLLGDDDTVLERYHPLRNFLVE540EDQCGKTTNGYTSVLINGHPSKLPIRDVNLLHLNTFNYELILGDVCYTTYLGKVVSLKHY600LNN 603<210>34<211>565<212>PRT<213>日本血吸虫(Schistosoma japonicum)<400>34MLAQGIFAHICCRLLRRNLGSQAVKPFFYQDILQLDKPPYAETPFRKLTGDFVSLKKIDD60KEILKIDSKALILLSQQAMIDVAHLLRPAHLQSLSNILRDPEASNNDKFVALELLKNANV120AAGMVLPGCQDTGTAICMAKKGQFVWTDGNDAEAISQGIYNTYTSSYLRYSQVAALDMFT180
EKNTGTNLPAQIEIYSTGGNEYSFLFIAKGGGSANKTFLYQQTKALLNPTSLYSFVNEKI240KTLGTAACPPYHLAVVVGGTSAEFTLKTVKLASCKYLDHLPSTGNEHGRAFRDREAEEKI300LHLTQKVGIGAQFGGKYFCHDVRVIRLPRHGASCPVGIGVSCSADRQILGRITNDGVFLE360QLETDPSQYLPDPSLNQLPTDVVKVNLNLPMKEILAELSKYPIKTRLSLTGTLVVARDIA420HAKIKERLDSGQPMPDYFCNHPVYYAGPAKTPMNYASGSFGPTTAGRMDSYVGQFQQAGG480SLIMLAKGNRSKAVTNACRKHGGFYLGSIGGPAAILAQNCIKKVEVLEYPELGMEAIWRV540EVEDFPAFIVVDDKGNDFFKEWQVE 565<210>35<211>429<212>PRT<213>日本血吸虫(Schistosoma japonicum)<400>35MHSPGIIVGMPFVEAILKESSCEIEWHICEGQPVKSCPVRVATITGANEDIFFCENLVLS60VLSRASGIASLASRIQSILQEVSWKGTMYMPDRRTPGFGLVEEYAMMISGISERKSSVSV120RCKDMDAGSLKSAIDEVRSRVGSIPVHVACCRLDEACLAAGAGADILLTGLSAKEIVDIA180SQVKEFLSRNPSLLPAALFDDSDVKLCATRYVDHLTSLKLCNGYAFGDFQMTYVRIVLEY240RNYKICPSGKKWSHDFISSHKDQILLDRKHLVVPFLQHVKSATYATNIHITRAPTMLLQP300GLNINNQVSGVLIPPNNQQNVNMRLPMIPAQLQQPPQQMPQRMPSNSPTVLGTNQPMGGG360FLNSIHPPHNQPPNWQVIPSGPSGHPMGGIGLGMRVSGPGGPNMNNCRLCGFPNPSSMPF420CRNCRSGLR 429<210>36<211>294<212>PRT<213>日本血吸虫(Schistosoma japonicum)<400>36MIIKEFRIILPLTVDEYRVGQLWAVAEASKNETGGGEGIEVLVNEPFDREVNPPESPLIA60NGEEFFSGQYTHKRFYLASKVPGYVRFLAPKGSLEVDEKAWNAYPYCRTILQNPKYMQDN120FTLIIESMHVQDKVNIENIHNLPPKLLAQREVIYIDIANDQLHIASDYDPSCDPRIYQSV180KTGRGPLVGPRWWENTDLPIMCAYKLVTCEFKWWGIQGRVENMIQVKKDGFFLTFNRQVF240CWLDKWHGLSMSDIRDVENKTKRNWTNNVLSVVFAALWLMIKMMSSLKLTILLF 294<210>37<211>295<212>PRT<213>日本血吸虫(Schistosoma japonicum)<400>37MKLKMLGIVMYRKNQATNFPSLITIFSAPNYLDVYNNKAAILKYENNTMNIRQFNCSPHP60YWLPNFMDVFTWSLPFVGEKVTEMLMNVLSICSDDELMKEGDDTFEGGTVAARKEVIRNK120IRAIGKMARTFTVLREESESVFELKGLTPNGMLPLGALSGGKESLNKALSGVSPRNKIIS180
FEQAKAYDQINERMPPRRDSSALATTPTSVRTFGSASRQSDKKPAPNPPGVAAVNAVDSN240YRRLSAKSNTSQAVTNENDIHRSSSTSGIRSCSNGETGINSLTKSSDPTSTRPRI 295<210>38<211>399<212>PRT<213>日本血吸虫(Schistosoma japonicum)<400>38MFQSKLNTMQKELEVSVNRADEEAENVEKLQIQLTKFTSELAQTKSKYEKVFSEKTEEFD60ELKRRLNNRINELTESYELERGRVVSLERTKNQLSHQCQEMQSQLDNCVGNAQSKLQTIK120SLESQKTELEAVLEEALVEESNLRTKCGLLQRDLIQIRATKSEMEERVTVLEKENKQNSE180KLKETKERLSVVNRQVTDLESSRARLEAERDNLDTTLRDTEDALHECEARYQTTFSALST240LRNEFERQSRDKEEELESLRRSSQRNVENLKAAITDMEMKNQNELDRLKKKLEGNINNLQ300VQLEEEKNAHLETIKVHEETEAHICQLEMNITELNNLVSETANTLQICESRRSALANEVE360GLRCQFGKSQKNWKRKLEEESCGKYVKVRWPELYSNNLT 399<210>39<211>600<212>PRT<213>日本血吸虫(Schistosoma japonicum)<400>39SSRAAKKQELIENLKRLFPGVHGRLLEMCQPSHRRYQVAITKVLGKYMDAIVCDSEKTAK60ECIQYMKDQRIEPETFLPLDFLDVKPIDEKLREISDPPNVHLVIDVIQCDPIIVKKALTF120ACGNALYVKLLNMLVMLPIIWEIEKKTVSLEGTLFQRSGVISGGASDLKARARRWDEKQI180STLMSNRDALQNELKEQLKRKRKEAELRTIQSQIKGLDTRLKYTLKDKDSTEEKLLSTNE240EEMNQIARELEEVEESLGRCQTKMQELQISVNAEKAKMDTVEDTVFHDFCAQIGVENIRQ300YEDRELRVARERDRKRLEFTNQLQRINNQLEYERSRDTEANVKRWEETVAVERTEMDKCR360NKKKRYKEEMEQEENKKTEIESRVGELKYRAEMLDGELGEIRRRLVNKQRDIQKLQKDLN420QAEAKLESRRAERHSLLQAAKMEDLELPLKPGCDPIPELSSQLTESENIDPSTEEMAHIY480ELEARLPIDFKHLDKPLRQMNDEKEVNRKAEEMQNQVDSMLNSLARIQAPNLRAGDKLGS540VEERLRSTEAEFEDTRRRAKRAKARFERVRRLRYNAFMNCFNSIADNIDPIYKSLSRNPG600<210>40<211>125<212>PRT<213>日本血吸虫(Schistosoma japonicum)<400>40MKSPKLTATPDSKSKQLSNGKTTPARPTSKRPIPVGSDDSDAETESNVLCNNELKTSHKK60SHSKVSFSGGPKGCCFKAEVFFRSKIKTCASRWTRTQNRCRLGFSGRCKEIRHKRVVQRS120SLSFA 125<210>41
<211>653<212>PRT<213>日本血吸虫(Schistosoma japonicum)<400>41CVTVGLLCKHFDADVFRSSKNANIRDQVFETLMFFTEQVKSDIEMRKKALSGLGFLCTRH60HELLCGPRLCKFYHDLLQAPCDEAKSKSTDHHLELKCIVLENLLNFFLEEEKRMLEADAK120WKASQHQESLKEMGDIASGMGSSVAQMYLKDILESFFSPFFTVRLTALSVITTIRRQGLV180HPVQTVPYLIAMRSDIDPNILIKLMLTSGIDTKFPGFLTMRAAQGVNLSYRLQLVLYDEI240REKLKVNSGGHCFQQLGSPEPSNIPKSTYSITATRRNIRSRADADSLSFDDNAKCSPTTN300VSESQSISNVPEDSYGAIRGTRDPFSEIPSALNSHLYSMLRNNKSQRRSLLNGLISLFDD360SQKIPLSQLVYVADNLAHFPYQSQEEPLFVVHHIDLMVSMAGSGVLNSVREALFPELKAA420LEAIVAAQLEADAKNRAQIELQLHAELQAQQVASVDATNRGDHETAGLFADQIQHTIQRI480NSLNSSNPIMRSNSSISDFSAGVIPGIEGVSRVDLGPFEVEEEEDPIALLDRLSKSRQTS540LPIVRDAIRTSRACVLLLTLKHFLKECYSITDSKIQRYSPSDSAKLWEKPLTRRVGIHFH600PIPCLKVAGNGIHKSDSHARYSSQSSIKTDHKFVDSSNPPYHKVDVKESAFMK 653<210>42<211>222<212>PRT<213>日本血吸虫(Schistosoma japonicum)<400>42MHCLFLNVIFLESINGVIRTMNCSSNLVRNISQLLSPLITEDELKHLEKLPREALSSKSS60HQDVTAFCLLKNAEDEVLRLFHKKEYFRKAYNDTLERMNTLANSLPSYALDYITTPGWHE120NLHNKDDDHDEEEDIPLDPDYVNFMKKTILHRQIRDNNNESTSIDIQSMLKAPPRKINKS180TDIQTIDAQIEMLEASLLHYYEFVSPCRDSPVWPILPLKRTL 222<210>43<211>131<212>PRT<213>日本血吸虫(Schistosoma japonicum)<400>43MRIAYSLKNAVEKMCDRKKVLLVKMKQLLKLKKDVILIICYINRLQSLVVAESTFLMMMM60VIIMTSLIVAPLKPSLTKLHIPRITKEYIATATLEIDTDVKCDAQAIFENAQKINQKSEN120RNVYMGLNNYA 131<210>44<211>210<212>PRT<213>日本血吸虫(Schistosoma japonicum)<400>44QPNIEQGKLQMWVDIFPTSLGEPGPPFDISPREANEYILRLVVWNTFDVVLDEKSITGEK60
MSDIYVKGWLAGLDDRQKTDVHYRSLNGEGNFNWRFVFPFFYLPAENTIVVKRKEHFWSM120DITEQRVRPQLVLQVWDNDLFSPDDFIGTLELNLSNMPSPSKSRSKCVMNMLQSIGNETK180MVNLFECRRLNGFLAICKRGKRTPLLTGKI 210<210>45<211>54<212>PRT<213>日本血吸虫(Schistosoma japonicum)<400>45VGLAYRDDAIGLYWLGQAFLKVLPCQAGRLKSGKTKSSKVELREQSCTADCTEV 54<210>46<211>94<212>PRT<213>日本血吸虫(Schistosoma japonicum)<400>46MIINNLFLLLRLLFIMRIIINIFVKILQFNALMQFLNPCMIHLHYFHLYIEQIDYSFINL60SYTYKHSITVLQQNFLLTLEIIQDVLLLLKAVSR 94<210>47<211>97<212>PRT<213>日本血吸虫(Schistosoma japonicum)<400>47MCCGDENSVSSSGSTSFGDADTYYVHHHDGSHILYEVFISCISIRNVSPTVYLFCSHIKI60DMLIKRAFLHLLIRNIIASNCVVYMFAVSICIKLYVF 97<210>48<211>73<212>PRT<213>日本血吸虫(Schistosoma japonicum)<400>48MIFLFGWGSQAFSGANYFFWIPIVGPYIGAIIGGVLYELTIGIHLDRKSDFVVDVDYDET60RSDGNKLMAYSSP 73<210>49<211>85<212>PRT<213>日本血吸虫(Schistosoma japonicum)<400>49MAVTLSGFQTESIFDADVKALIDMARAAFRQLKNIWDFNHLSVNIKVSIFNTNVKTLQLY60
GAETCMKKCKKHYSKNTSIYELLFE 85<210>50<211>94<212>PRT<213>日本血吸虫(Schistosoma japonicum)<400>50MRMVCHIALLLSVVMIVSFDASVSGEYNLTKDDVGPFLLGVAMGEASSDESSPVETRGSK60KKKKNKKNGNKSSNDSKEKNKANAATFPRTTYSS 94<210>51<211>81<212>PRT<213>日本血吸虫(Schistosoma japonicum)<400>51MCSTQSNCIICIDNSRNPNAEKRLKSTWAPIIPFYMYMSKIFSKNEKGRSNKKRIGERTR60EYVLNVLGHSIYCRFLVNNTI 81<210>52<211>57<212>PRT<213>日本血吸虫(Schistosoma japonicum)<400>52MLNKSRLWLVSDRNLSKFAPISVKFAMVKHILRNSYTPSISYLGSKQYICLFRYVTL 57<210>53<211>79<212>PRT<213>日本血吸虫(Schistosoma japonicum)<400>53KLAVIAHLEKTTGENLANSALFNPKEEVTARSSCSAGDTKQTKNDDIKVEQSNSNTAARI60VFRRRPASNRSIRTSQDDE 79<210>54<211>59<212>PRT<213>日本血吸虫(Schistosoma japonicum)<400>54YLDVVVGLAYRDDAIGLYWLGQAFLKVLPCQAGRLKSGKTKSSKFELREQSCTADCTEV 59
权利要求
1.一类日本血吸虫表皮抗原基因，其编码具有日本血吸虫表皮抗原蛋白质活性的核苷酸序列，所述的核苷酸序列包括(a)SEQ ID No.1～SEQ ID No.27所示的序列；(b)SEQ ID No.1～SEQ ID No.27所示的序列中每条序列的互补序列；(c)与SEQ ID No.1～SEQ ID No.27所示的序列中每条序列有至少70％同源性的序列，及(d)上述(a)～(c)中一条或数条的组合。
2.如权利要求1所述的一类日本血吸虫表皮抗原基因的核苷酸序列，其特征在于所述的核苷酸序列包括具有SEQ ID No.1～SEQ ID No.27所示的序列。
3.一类日本血吸虫表皮抗原蛋白，包括(a)具有SEQ ID No.28～SEQ ID No.54所示氨基酸序列的蛋白；(b)由(a)的蛋白发生一个或几个氨基酸置换、缺失或插入而形成的具有(a)蛋白相同活性的蛋白。
4.如权利要求3所述的日本血吸虫表皮抗原蛋白，其特征在于，其具有SEQ IDNo.28～SEQ ID No.54所示的氨基酸序列。
5.一类抗体，其特征在于，所述抗体能与权利要求3所述的蛋白特异性结合。
6.权利要求3所述的日本血吸虫表皮抗原蛋白在制备血吸虫疫苗中的应用。
7.权利要求3所述的日本血吸虫表皮抗原蛋白在制备防治血吸虫病的药物中的应用。
全文摘要
本发明公开了一类日本血吸虫表皮抗原基因及其编码蛋白，还公开了与该日本血吸虫表皮抗原蛋白特异结合的抗体。此外，本发明还公开了此类日本血吸虫表皮抗原蛋白在制备血吸虫疫苗中的应用，以及在制备防治血吸虫病的药物中的应用。运用本发明的日本血吸虫表皮抗原蛋白，可研究血吸虫治疗药物的作用靶点，从而在开发研制预防或治疗血吸虫病的药物过程中发挥重要作用。
文档编号C07K16/18GK1986798SQ200510111718
公开日2007年6月27日 申请日期2005年12月20日 优先权日2005年12月20日
发明者刘锋, 韩泽广, 王志勤, 胡薇, 冯正 申请人:上海人类基因组研究中心, 中国疾病预防控制中心寄生虫病预防控制所