自身免疫抗体的制作方法

自身免疫抗体的制作方法
【专利摘要】本发明一般涉及与NSCLC有关的生物标志物，及用于在人受试者中检测和诊断非小细胞肺癌的方法和组合物。
【专利说明】自身免疫抗体 发明领域
[0001] 本发明提供用于鉴定会最多地受益于厄洛替尼（Erlotinib)治疗的诊断有非小 细胞肺癌的患者的方法，其包括在所述患者的血清中检测自身抗体。
[0002] 发明背景
[0003] Tarceva?是一种有口服活性的、有力的表皮生长因子受体（EGFR)酪氨酸激酶 (TKI)抑制剂。
[0004] 厄洛替尼盐酸盐是:Tarceva?中的活性组分，Tarceva?批准作为单药治疗在化 疗后用于晚期非小细胞肺癌（NSCLC)患者，作为维持治疗用于化疗期间无发展（一线维持） 或化疗失败后（二/三线维持）的患者。Tarceva?还批准作为一线治疗用于其肿瘤在EU 中包含EGFR激活性突变的患者。
[0005] 不寻常蛋白质的表达在癌症和病毒感染中是常见的。哺乳动物免疫系统含有一 个专门分支，其识别由细胞转化诱导的蛋白质（新抗原（neo-antigen)并有效消除表达这 些新抗原的细胞，发育期间没有建立针对新抗原的耐受。认为免疫系统的这个分支针对病 毒感染、由细胞分裂机制的错误引起的自发染色体和基因组重排、或致癌原诱导的转化性 突变是有效的。虽然初始细胞毒性免疫应答-其通过CD8+T细胞对MHCI复合物上展示的 不寻常蛋白质的识别介导-快速且有效，针对病毒感染或异常细胞克隆的持续应答需要 CD4+T辅助细胞的共刺激效应，CD4+T辅助细胞经专门抗原呈递细胞的MHCII复合物在呈递 胞外肽（其可以源于在细胞毒性免疫应答的第一阶段中裂解的细胞）后激活。另外，这些 细胞能够诱导持久的针对病毒或异常蛋白质的B细胞应答和抗体。已经在结直肠癌中显示 大量⑶8+T细胞浸润肿瘤是比传统肿瘤分期更好的预后标志物（Koizumi，Hojo等2007 1)， 而且已经在几乎所有主要实体癌中将它与有利的预后关联起来，不管癌细胞类型。
[0006] 自身抗体是公知的，癌症中的诊断性实体。许多癌症自身抗体是正常情况下在胚 胎组织中表达的蛋白质，因此代表"外来"蛋白质，没有建立针对它们的免疫耐受。癌症史 晚期这些蛋白质脱落到血流中引起体液免疫应答。一个典型的例子是常见的癌症标志物 CEA(癌胚抗原）。肿瘤频繁展现错误的蛋白质加工。突出的例子包括受到细胞定位蛋白酶 不正确切割（存在针对P53N端序列的自身抗体是肺癌最特异性的生物标志物之一，正常情 况下在该蛋白质靶定至核后切割N端序列）或受到细胞糖基化酶不正确糖基化（针对不正 确〇-糖基化形式的MUCl的抗MUCl抗体是多种癌症的生物标志物）的蛋白质。尽管在肿 瘤中频繁观察到P53突变，但是自身抗体针对这种蛋白质的特异性几乎总是针对该蛋白质 的N端-更多地将p53的免疫原性与正常情况下短命且定位于核的蛋白质的异位表达联 系起来。
[0007] Li等2记载了 NSCLC患者的血清中天然EGFR自身抗体的检测。Li等3和Chapman 等4记载了针对p53、HER2、NY-ES0-l、CAGE、MUCl和GBU4-5的天然自身抗体的检测。而且， W020110739055涉及与癌症疾病自较低的发展阶段发展至较高的发展阶段有关的肿瘤标志 物。
[0008] 不寻常（突变的或以异常数量或定位表达的）肿瘤蛋白质能引发抗体应答。已 经频繁观察到肿瘤诱导的自身抗体，而且已经调查它们作为诊断标志物的效用（Albert和 Darnell 20046)。虽然能找到具有卓越特异性的针对肿瘤蛋白质的自身抗体，但是它们大 多数缺乏诊断测试需要的灵敏度。
[0009] 本领域知道少数可靠的预测厄洛替尼盐酸盐响应的流行病学和遗传标志物。特别 地，记载了外显子18-21 (受体的胞内激酶域）中的EGFR突变与较好的预后以及与较好的 对TKI治疗的响应有联系（Paz-Ares，Soulieres等2010 7)。当前知道的唯一预测标志物 (EGFR激活性突变）难以诊断，因为需要肿瘤活检。目前，只有约50 %的NSCLC患者是以 活检诊断的（Reck，Hermes等20118)。明确需要更简单的技术来检测TKI功效的预测标志 物。想要在诊断时鉴定响应者，使得有尽可能多的患者尽可能早地受益于厄洛替尼治疗，同 时为不太可能响应的患者探索其它治疗选项。
[0010] 发明详述
[0011] 本发明解决的问题在于提供一种在人受试者中诊断癌症的方法。
[0012] 本发明解决的问题在于提供一种在人受试者中诊断非小细胞肺癌的方法。
[0013] 本发明解决的问题在于提供一种在人受试者中诊断非小细胞肺癌的方法，其通过 提供针对突变型EGFR序列的抗体来进行。
[0014] 通过本文所述方法鉴定的患者为会响应厄洛替尼治疗或其药学可接受盐，特别是 厄洛替尼盐酸盐的患者，特别是NSCLC患者。
[0015] 我们令人惊讶地发现本发明的自身抗体拥有诊断测试需要的灵敏度，尤其是针对 突变型EGFR序列的自身抗体。
[0016] EGFR正常情况下结合至细胞膜且不脱落入血流。EGFR是一种正常成体蛋白质，在 一些成体组织中已大量找到。预期会建立针对这种蛋白质和它的许多变体的免疫耐受。几 乎所有序列属于分子的胞质部分且专门抗原呈递细胞看不到。突变仅仅影响EGFR分子的 一小部分。
[0017] 除非另有定义，本文中使用的所有术语具有与本发明所属领域技术人员普遍理解 相同的含义。
[0018] 术语"代表健康群体人受试者的所述自身抗体的水平"指未罹患NSCLC的患者群 体的血清中自身抗体的均值水平的估值。
[0019] 术语"代表NSCLC患者的所述自身抗体的水平"指罹患NSCLC的患者群体的血清 中自身抗体的均值水平的估值。
[0020] 术语"总体存活（OS) "指自疾病诊断起，治疗期间和之后患者存活的时间长度。正 如技术人员会领会的，如果患者属于与另一个患者亚组相比具有统计学显著更长的均值存 活时间的一个患者亚组，那么该患者的总体存活得到改善或增强。
[0021] 术语"无进展存活（PFS) "指自疾病诊断起，治疗期间和之后，根据主治医师或调查 人员的评估，患者的疾病没有变得更差，即没有发展的时间长度。正如技术人员会领会的， 如果患者属于与情况相似患者的对照组的平均或均值无进展存活时间相比有更长的时间 疾病没有发展的一个患者亚组，那么该患者的无进展存活得到改善或增强。
[0022] 术语"患者"指想要治疗的任何单一哺乳动物。特别地，"患者"为人受试者。更特 别地，患者为罹患癌症，特别是NSCLC的人受试者。
[0023] 术语"自身抗体"为患者免疫系统针对一种或多种患者自身蛋白质制造的一类蛋 白质。
[0024] 术语"氨基酸"表示一组天然发生的羧基α-氨基酸，包括丙氨酸（三字母代码： ala，单字母代码：Α)、精氨酸（arg，R)、天冬酰胺（asn，Ν)、天冬氨酸（asp, D)、半胱氨酸 (cys，〇、谷氨酰胺（gln，Q)、谷氨酸（glu，E)、甘氨酸（gly，G)、组氨酸（his，H)、异亮氨 酸（ile，I)、亮氨酸（leu，L)、赖氨酸（lys，K)、甲硫氨酸（met, M)、苯丙氨酸（phe，F)、脯氨 酸（pro, P)、丝氨酸（ser，S)、苏氨酸（thr，T)、色氨酸（trp，W)、酪氨酸（tyr，Y)、和纟颜氨酸 (val, V)。
[0025] 如本文中使用的，"疗法"或"治疗"（及其语法变型）指试图改变所治疗个体中疾 病的自然过程的临床干预，而且可以为了预防或在临床病理过程期间实施。想要的治疗效 果包括但不限于预防疾病发生或再发生、缓解症状、削弱疾病的任何直接或间接病理后果、 预防转移、降低疾病发展速率、改善或减轻疾病状态、和消除或改善预后。
[0026] 如本文中使用的，术语"基因"包括基因的变体。术语"变体"涉及与通过GenBank 登录号给出的核酸序列实质性相似的核酸序列。本领域技术人员能较好地理解术语"实质 性相似"。特别地，基因变体可以是与人群中最流行的等位基因的核酸序列相比显示核苷酸 交换的等位基因。优选地，此类实质性相似核酸序列与最流行的等位基因具有至少80 %，优 选至少85 %，更优选至少90 %，最优选至少95 %的序列相似性。术语"变体"还意图涉及剪 接变体。
[0027] 术语"突变"指基因组序列中的变化。这些随机序列可以定义为细胞中的突发和 自发变化。突变可导致序列中数种不同类型的变化；这些可以没有影响，改变基因的产物， 或阻止基因正确或完全发挥功能。术语"体细胞突变"指遗传结构中既不是继承得到的也 不会传给后代的变化。
[0028] 如本文中使用的，短语"推荐治疗"指使用所生成的涉及患者的样品中生物标志物 或自身抗体的水平或存在的信息或数据来鉴定患者为适合或不适合用某种疗法治疗。在一 些实施方案中，所述疗法可包含药物。所述信息或数据可以是任何形式，书面的、口头的或 电子的。在一些实施方案中，使用所生成的信息或数据包括传达、呈现、报告、存储、发送、转 移、供应、传输、递送、分发、或其组合。在一些实施方案中，传达、呈现、报告、存储、发送、转 移、供应、传输、递送、分发、或其组合是由计算装置、分析单元或其组合实施的。在一些进一 步的实施方案中，传达、呈现、报告、存储、发送、转移、供应、传输、递送、分发、或其组合是由 实验室或医学专业人员实施的。在一些实施方案中，所述信息或数据包括所述生物标志物 或自身抗体的水平与参照水平的比较。在一些实施方案中，所述信息或数据包括样品中存 在或缺失所述生物标志物或自身抗体的指示。在一些实施方案中，所述信息或数据包括患 者适合或不适合用包含所述药物的疗法治疗的指示。在一些实施方案中，所述疗法为厄洛 替尼。
[0029] 如本文中使用的，短语"提供诊断"指使用所生成的涉及患者的样品中生物标志物 或自身抗体的水平或存在的信息或数据来诊断患者中的疾病。所述信息或数据可以是任何 形式，书面的、口头的或电子的。在一些实施方案中，使用所生成的信息或数据包括呈现、报 告、存储、发送、转移、供应、传输、递送、分发、或其组合。在一些实施方案中，呈现、报告、存 储、发送、转移、供应、传输、递送、分发、或其组合是由计算装置、分析单元或其组合实施的。 在一些实施方案中，呈现、报告、存储、发送、转移、供应、传输、递送、分发、或其组合是由实 验室或医学专业人员实施的。在一些实施方案中，所述信息或数据包括所述生物标志物或 自身抗体的水平与参照水平的比较。在一些实施方案中，所述信息或数据包括样品中存在 或缺失所述生物标志物或自身抗体的指示。在一些实施方案中，所述信息或数据包括患者 诊断有所述疾病的指示。在一些实施方案中，所述疾病为非小细胞肺癌。
[0030] 在TASK研究期间，收集了活检材料并对肿瘤细胞测试了最频繁的体细胞突变的 存在情况，即外显子19删除和外显子21点突变。在TASK研究期间，在多个时间点（给药 前、第8天、第22天和发展）自所有患者收集了血清样品并评估了针对EGFR的自身抗体。
[0031] 在这些患者中，预测疹发生或无进展或总体存活延长的自身抗原性肽序列必然包 括一套自EGFR序列衍生，始于第737位且延伸通过第756位的序列。这些肽包括一些序列 变体，但是必然包括具有第746-750位或附近处的删除的序列（表2)。
[0032] 这恰好对应于外显子19体细胞突变中的删除的位置，已知其预示源于TKI治疗的 突出功效（Rosell 等 20099, Mok 等 20091°)。
[0033] 肿瘤组织中外显子19中存在体细胞EGFR突变产生蛋白质变体，免疫系统未针对 它形成耐受。该体细胞突变仅仅发生在肿瘤中，因此，如果它诱导自身抗体，在患者的血清 中能检测到的话，它可用于得出关于NSCLC组织中存在外显子19突变或外显子21突变 的结论，公知它预测无进展存活延长和酪氨酸激酶单一疗法优于化疗（Heigener和Reck 201111)。
[0034] 可使用来自患者的标准血液样品检测自身抗体，无需以活检获得肿瘤细胞。这相 对于当前实践是一个较大优点，因为有用肿瘤样品的回收率甚至在临床研究中也不超过 50% (Reck，Hermes 等 201112)。
[0035] 能检测出抗EGFR自身抗体在NSCLC患者的血液样品中的浓度比健康对照中高 (图1)。特别地，已经鉴定出肽序列，产生具有高免疫原性的连续区域，在患者和健康对照 血清之间具有较大差异。鉴定了连续序列段，其中超过30%的癌症患者中各种肽信号对对 照中的最大值的比大于8(表1)。
[0036]
【权利要求】
1. 一种在人受试者中诊断非小细胞肺癌的方法，其包括： 在该人受试者的血液样品中测量选自识别突变型人EGFR的自身抗体组的自身抗体的 水平，其中该人受试者的血液样品中所述选自识别突变型人EGFR的自身抗体组的自身抗 体的水平与代表健康群体人受试者的所述自身抗体的水平相比升高指示非小细胞肺癌。
2. 依照权利要求1的方法，其中所述自身抗体识别选自下组的突变型EGFR肽： Seq. Id. No. 554, Seq. Id. No. 555, Seq. Id. No. 556, Seq. Id. No. 557, Seq. Id. No. 558, Seq. Id. No. 559,和 Seq. Id. No. 560。
3. 依照权利要求1-2任一项的方法，其中所述自身抗体识别选自下组的突变型EGFR 肽： Seq. Id. No. 1, Seq. Id. No. 2, Seq. Id. No. 4, Seq. Id. No. 5, Seq. Id. No. 6, Seq. Id. No. 7, Seq. Id. No. 15，Seq. Id. No. 16，Seq. Id. No. 17，Seq. Id. No. 18，Seq. Id. No. 19，Seq. Id. No. 20，Seq. Id. No. 21，Seq. Id. No. 22，Seq. Id. No. 23，Seq. Id. No. 24，Seq. Id. No. 25，Seq. Id. No. 26，Seq. Id. No. 27，Seq. Id. No. 28，Seq. Id. No. 29，Seq. Id. No. 30，Seq. Id. No. 31， Seq. Id. No. 32，Seq. Id. No. 33，Seq. Id. No. 34，Seq. Id. No. 35，Seq. Id. No. 36，Seq. Id. No. 37，Seq. Id. No. 38，Seq. Id. No. 39，Seq. Id. No. 40，Seq. Id. No. 41，Seq. Id. No. 42，Seq. Id. No. 43，Seq. Id. No. 44，Seq. Id. No. 45，Seq. Id. No. 46，Seq. Id. No. 47，Seq. Id. No. 48， Seq. Id. No. 49，Seq. Id. No. 50，Seq. Id. No. 51，Seq. Id. No. 52，Seq. Id. No. 53，Seq. Id. No. 54，Seq. Id. No. 55，Seq. Id. No. 56，Seq. Id. No. 57，Seq. Id. No. 58，Seq. Id. No. 59，Seq. Id. No. 60，Seq. Id. No. 61，Seq. Id. No. 62，Seq. Id. No. 63，Seq. Id. No. 65，Seq. Id. No. 66， Seq. Id. No. 67，Seq. Id. No. 68，Seq. Id. No. 69，Seq. Id. No. 70，Seq. Id. No. 71，Seq. Id. No. 72, Seq. Id. No. 73, Seq. Id. No. 74, Seq. Id. No. 75, Seq. Id. No. 76, Seq. Id. No. 77, Seq. Id. No. 78，Seq. Id. No. 79，Seq. Id. No. 80，Seq. Id. No. 81，Seq. Id. No. 82，Seq. Id. No. 83， Seq. Id. No. 84，Seq. Id. No. 85，Seq. Id. No. 86，Seq. Id. No. 87，Seq. Id. No. 88，Seq. Id. No. 89，Seq. Id. No. 90，Seq. Id. No. 91，Seq. Id. No. 92，Seq. Id. No. 93，Seq. Id. No. 94，Seq. Id. No. 95，Seq. Id. No. 96，Seq. Id. No. 97，Seq. Id. No. 98，Seq. Id. No. 99，Seq. Id. No. 100， Seq. Id. No. 101, Seq. Id. No. 103, Seq. Id. No. 104, Seq. Id. No. 105, Seq. Id. No. 106, Seq. Id. No. 107, Seq. Id. No. 108, Seq. Id. No. 109, Seq. Id. No. 110, Seq. Id. No. Ill, Seq. Id. No. 112, Seq. Id. No. 113, Seq. Id. No. 114, Seq. Id. No. 115, Seq. Id. No. 116, Seq. Id. No. 117, Seq. Id. No. 118, Seq. Id. No. 119, Seq. Id. No. 120, Seq. Id. No. 121, Seq. Id. No. 122, Seq. Id. No. 123, Seq. Id. No. 124, Seq. Id. No. 125, Seq. Id. No. 126, Seq. Id. No. 127, Seq. Id. No. 128, Seq. Id. No. 129, Seq. Id. No. 130, Seq. Id. No. 131, Seq. Id. No. 132, Seq. Id. No. 133, Seq. Id. No. 134, Seq. Id. No. 135, Seq. Id. No. 136, Seq. Id. No. 137, Seq. Id. No. 138, Seq. Id. No. 139, Seq. Id. No. 140, Seq. Id. No. 141, Seq. Id. No. 142, Seq. Id. No. 143, Seq. Id. No. 144, Seq. Id. No. 145, Seq. Id. No. 146, Seq. Id. No. 147, Seq. Id. No. 148, Seq. Id. No. 149, Seq. Id. No. 150, Seq. Id. No. 151, Seq. Id. No. 152, Seq. Id. No. 153, Seq. Id. No. 154, Seq. Id. No. 155, Seq. Id. No. 156, Seq. Id. No. 157, Seq. Id. No. 158, Seq. Id. No. 159, Seq. Id. No. 160, Seq. Id. No. 161, Seq. Id. No. 162, Seq. Id. No. 163, Seq. Id. No. 164, Seq. Id. No. 165, Seq. Id. No. 166, Seq. Id. No. 167, Seq. Id. No. 168, Seq. Id. No. 169, Seq. Id. No. 170, Seq. Id. No. 171，Seq. Id. No. 172, Seq. Id. No. 173, Seq. Id. No. 174, Seq. Id. No. 175, Seq. Id. No. 176, Seq. Id. No. 177, Seq. Id. No. 178, Seq. Id. No. 179, Seq. Id. No. 180, Seq. Id. No. 181Seq. Id. No. 182, Seq. Id. No. 183, Seq. Id. No. 184, Seq. Id. No. 185, Seq. Id. No. 186, Seq. Id. No. 187， Seq. Id. No. 188, Seq. Id. No. 189, Seq. Id. No. 190, Seq. Id. No. 191, Seq. Id. No. 192, Seq. Id. No. 193, Seq. Id. No. 194, Seq. Id. No. 195, Seq. Id. No. 196, Seq. Id. No. 197, Seq. Id. No. 198, Seq. Id. No. 199, Seq. Id. No. 200, Seq. Id. No. 201, Seq. Id. No. 202, Seq. Id. No. 203，Seq. Id. No. 204，Seq. Id. No. 205，Seq. Id. No. 206，Seq. Id. No. 207，Seq. Id. No. 208, Seq. Id. No. 209, Seq. Id. No. 210, Seq. Id. No. 211, Seq. Id. No. 212, Seq. Id. No. 213, Seq. Id. No. 214, Seq. Id. No. 215, Seq. Id. No. 216, Seq. Id. No. 217, Seq. Id. No. 218, Seq. Id. No. 219, Seq. Id. No. 220, Seq. Id. No. 221, Seq. Id. No. 222, Seq. Id. No. 223，Seq. Id. No. 224，Seq. Id. No. 225，Seq. Id. No. 226，Seq. Id. No. 227，Seq. Id. No. 228, Seq. Id. No. 229, Seq. Id. No. 230, Seq. Id. No. 231, Seq. Id. No. 232, Seq. Id. No. 233，Seq. Id. No. 234，Seq. Id. No. 235，Seq. Id. No. 236，Seq. Id. No. 237，Seq. Id. No. 238, Seq. Id. No. 239, Seq. Id. No. 240, Seq. Id. No. 241, Seq. Id. No. 242, Seq. Id. No. 243, Seq. Id. No. 244, Seq. Id. No. 245, Seq. Id. No. 246, Seq. Id. No. 247, Seq. Id. No. 248, Seq. Id. No. 249, Seq. Id. No. 250, Seq. Id. No. 251, Seq. Id. No. 252, Seq. Id. No. 253，Seq. Id. No. 254，Seq. Id. No. 255，Seq. Id. No. 256，Seq. Id. No. 257，Seq. Id. No. 258，Seq. Id. No. 259，Seq. Id. No. 260，Seq. Id. No. 261，Seq. Id. No. 262，Seq. Id. No. 263，Seq. Id. No. 264，Seq. Id. No. 265，Seq. Id. No. 266，Seq. Id. No. 267，Seq. Id. No. 268, Seq. Id. No. 269, Seq. Id. No. 270, Seq. Id. No. 271, Seq. Id. No. 272, Seq. Id. No. 273, Seq. Id. No. 274, Seq. Id. No. 275, Seq. Id. No. 276, Seq. Id. No. 277, Seq. Id. No. 278, Seq. Id. No. 279, Seq. Id. No. 280, Seq. Id. No. 281, Seq. Id. No. 282, Seq. Id. No. 283，Seq. Id. No. 284，Seq. Id. No. 285，Seq. Id. No. 286，Seq. Id. No. 287，Seq. Id. No. 288，Seq. Id. No. 289，Seq. Id. No. 290，Seq. Id. No. 291，Seq. Id. No. 292，Seq. Id. No. 293，Seq. Id. No. 294，Seq. Id. No. 295，Seq. Id. No. 296，Seq. Id. No. 297，Seq. Id. No. 298，Seq. Id. No. 299，Seq. Id. No. 300，Seq. Id. No. 301，Seq. Id. No. 302，Seq. Id. No. 303，Seq. Id. No. 304，Seq. Id. No. 305，Seq. Id. No. 306，Seq. Id. No. 307，Seq. Id. No. 308, Seq. Id. No. 310, Seq. Id. No. 311, Seq. Id. No. 312, Seq. Id. No. 313, Seq. Id. No. 314, Seq. Id. No. 315, Seq. Id. No. 316, Seq. Id. No. 317, Seq. Id. No. 318, Seq. Id. No. 319, Seq. Id. No. 320, Seq. Id. No. 321, Seq. Id. No. 322, Seq. Id. No. 323, Seq. Id. No. 324，Seq. Id. No. 325，Seq. Id. No. 326，Seq. Id. No. 327，Seq. Id. No. 328，Seq. Id. No. 329，Seq. Id. No. 330，Seq. Id. No. 331，Seq. Id. No. 332，Seq. Id. No. 333，Seq. Id. No. 334，Seq. Id. No. 335，Seq. Id. No. 336，Seq. Id. No. 337，Seq. Id. No. 338，Seq. Id. No. 339, Seq. Id. No. 340, Seq. Id. No. 341, Seq. Id. No. 342, Seq. Id. No. 343, Seq. Id. No. 344, Seq. Id. No. 345, Seq. Id. No. 346, Seq. Id. No. 347, Seq. Id. No. 348, Seq. Id. No. 349，Seq. Id. No. 350，Seq. Id. No. 351，Seq. Id. No. 352，Seq. Id. No. 353，Seq. Id. No. 354，Seq. Id. No. 355，Seq. Id. No. 356，Seq. Id. No. 357，Seq. Id. No. 358，Seq. Id. No. 359, Seq. Id. No. 360, Seq. Id. No. 361, Seq. Id. No. 362, Seq. Id. No. 363, Seq. Id. No. 364，Seq. Id. No. 365，Seq. Id. No. 366，Seq. Id. No. 367，Seq. Id. No. 368，Seq. Id. No. 369, Seq. Id. No. 371, Seq. Id. No. 372, Seq. Id. No. 374, Seq. Id. No. 375, Seq. Id. No. 376, Seq. Id. No. 377, Seq. Id. No. 378, Seq. Id. No. 379, Seq. Id. No. 380, Seq. Id. No. 381, Seq. Id. No. 382, Seq. Id. No. 383, Seq. Id. No. 384, Seq. Id. No. 385, Seq. Id. No. 386, Seq. Id. No. 387, Seq. Id. No. 388, Seq. Id. No. 389, Seq. Id. No. 390, Seq. Id. No. 391, Seq. Id. No. 392, Seq. Id. No. 393, Seq. Id. No. 394, Seq. Id. No. 395, Seq. Id. No. 396, Seq. Id. No. 397, Seq. Id. No. 398, Seq. Id. No. 399, Seq. Id. No. 400, Seq. Id. No. 401, Seq. Id. No. 402, Seq. Id. No. 403, Seq. Id. No. 404, Seq. Id. No. 405, Seq. Id. No. 406, Seq. Id. No. 407, Seq. Id. No. 408, Seq. Id. No. 409, Seq. Id. No. 410, Seq. Id. No. 411, Seq. Id. No. 412, Seq. Id. No. 413, Seq. Id. No. 414, Seq. Id. No. 415, Seq. Id. No. 416, Seq. Id. No. 417, Seq. Id. No. 418, Seq. Id. No. 419, Seq. Id. No. 420, Seq. Id. No. 421, Seq. Id. No. 422, Seq. Id. No. 423, Seq. Id. No. 424, Seq. Id. No. 425, Seq. Id. No. 426, Seq. Id. No. 427, Seq. Id. No. 428, Seq. Id. No. 429, Seq. Id. No. 430, Seq. Id. No. 431, Seq. Id. No. 432, Seq. Id. No. 433, Seq. Id. No. 434, Seq. Id. No. 435, Seq. Id. No. 436, Seq. Id. No. 437, Seq. Id. No. 438, Seq. Id. No. 439, Seq. Id. No. 440, Seq. Id. No. 441, Seq. Id. No. 442, Seq. Id. No. 443, Seq. Id. No. 444, Seq. Id. No. 445, Seq. Id. No. 446, Seq. Id. No. 447, Seq. Id. No. 448, Seq. Id. No. 449, Seq. Id. No. 450, Seq. Id. No. 451, Seq. Id. No. 452, Seq. Id. No. 453, Seq. Id. No. 454, Seq. Id. No. 455, Seq. Id. No. 456, Seq. Id. No. 457, Seq. Id. No. 458, Seq. Id. No. 459, Seq. Id. No. 460, Seq. Id. No. 461, Seq. Id. No. 462, Seq. Id. No. 463, Seq. Id. No. 464, Seq. Id. No. 465, Seq. Id. No. 466, Seq. Id. No. 467, Seq. Id. No. 468, Seq. Id. No. 469, Seq. Id. No. 470, Seq. Id. No. 471, Seq. Id. No. 472, Seq. Id. No. 473, Seq. Id. No. 474, Seq. Id. No. 475, Seq. Id. No. 476, Seq. Id. No. 477, Seq. Id. No. 478, Seq. Id. No. 479, Seq. Id. No. 480, Seq. Id. No. 481, Seq. Id. No. 482, Seq. Id. No. 483, Seq. Id. No. 484, Seq. Id. No. 485, Seq. Id. No. 486, Seq. Id. No. 487, Seq. Id. No. 488, Seq. Id. No. 489, Seq. Id. No. 490, Seq. Id. No. 491, Seq. Id. No. 492, Seq. Id. No. 493, Seq. Id. No. 494, Seq. Id. No. 495, Seq. Id. No. 496, Seq. Id. No. 497, Seq. Id. No. 498, Seq. Id. No. 499, Seq. Id. No. 500, Seq. Id. No. 501, Seq. Id. No. 502, Seq. Id. No. 503, Seq. Id. No. 504, Seq. Id. No. 505, Seq. Id. No. 506, Seq. Id. No. 507, Seq. Id. No. 508, Seq. Id. No. 509, Seq. Id. No. 510, Seq. Id. No. 511, Seq. Id. No. 512, Seq. Id. No. 513, Seq. Id. No. 514, Seq. Id. No. 515, Seq. Id. No. 516, Seq. Id. No. 517, Seq. Id. No. 518, Seq. Id. No. 522, Seq. Id. No. 523, Seq. Id. No. 524, Seq. Id. No. 526, Seq. Id. No. 527, Seq. Id. No. 528, Seq. Id. No. 529, Seq. Id. No. 530, Seq. Id. No. 531, Seq. Id. No. 532, Seq. Id. No. 533, Seq. Id. No. 534, Seq. Id. No. 535, Seq. Id. No. 536, Seq. Id. No. 537, Seq. Id. No. 538, Seq. Id. No. 539, Seq. Id. No. 540, Seq. Id. No. 541, Seq. Id. No. 542, Seq. Id. No. 543, Seq. Id. No. 544, Seq. Id. No. 545, Seq. Id. No. 546, Seq. Id. No. 547, Seq. Id. No. 548, Seq. Id. No. 549, Seq. Id. No. 550, Seq. Id. No. 551, Seq. Id. No. 552, Seq. Id. No. 553, Seq. Id. No. 554, Seq. Id. No. 555, Seq. Id. No. 557, Seq. Id. No. 559, Seq. Id. No. 560, Seq. Id. No. 562, Seq. Id. No. 563, Seq. Id. No. 564, Seq. Id. No. 565, Seq. Id. No. 567, Seq. Id. No. 568, Seq. Id. No. 569, Seq. Id. No. 570, Seq. Id. No. 571, Seq. Id. No. 572, Seq. Id. No. 573, Seq. Id. No. 574, Seq. Id. No. 575, Seq. Id. No. 576, Seq. Id. No. 577, Seq. Id. No. 578, Seq. Id. No. 579，Seq. Id. No. 580，Seq. Id. No. 581，Seq. Id. No. 582，Seq. Id. No. 583，Seq. Id. No. 584，Seq. Id. No. 585，Seq. Id. No. 586，Seq. Id. No. 587，Seq. Id. No. 588，Seq. Id. No. 589，Seq. Id. No. 590，Seq. Id. No. 591，Seq. Id. No. 592，Seq. Id. No. 593，Seq. Id. No. 594，Seq. Id. No. 595，Seq. Id. No. 596，Seq. Id. No. 597，Seq. Id. No. 598，Seq. Id. No. 599，Seq. Id. No. 601，Seq. Id. No. 604，Seq. Id. No. 605，Seq. Id. No. 606，Seq. Id. No. 607, Seq. Id. No. 608, Seq. Id. No. 609, Seq. Id. No. 610, Seq. Id. No. 611, Seq. Id. No. 612, Seq. Id. No. 613, Seq. Id. No. 614, Seq. Id. No. 615, Seq. Id. No. 616, Seq. Id. No. 617, Seq. Id. No. 618 和 Seq. Id. No. 619。
4. 依照权利要求1-3任一项的方法，其中所述自身抗体识别选自下组的突变型EGFR 肽：Seq. Id. No. 1，Seq. Id. No. 2, Seq. Id. No. 4, Seq. Id. No. 5, Seq. Id. No. 6, Seq. Id. No. 7 和 Seq. Id. No. 15。
5. 依照权利要求1-3任一项的方法，其中所述自身抗体识别选自下组的突变型EGFR 肽： Seq. Id. No. 16，Seq. Id. No. 17，Seq. Id. No. 18，Seq. Id. No. 19，Seq. Id. No. 20，Seq. Id. No. 21，Seq. Id. No. 22，Seq. Id. No. 23，Seq. Id. No. 24，Seq. Id. No. 25，Seq. Id. No. 26， Seq. Id. No. 27，Seq. Id. No. 28，Seq. Id. No. 29，Seq. Id. No. 30，Seq. Id. No. 31，Seq. Id. No. 32，Seq. Id. No. 33，Seq. Id. No. 34，Seq. Id. No. 35，Seq. Id. No. 36，Seq. Id. No. 37， Seq. Id. No. 38，Seq. Id. No. 39，Seq. Id. No. 40，Seq. Id. No. 41，Seq. Id. No. 42，Seq. Id. No. 43，Seq. Id. No. 44，Seq. Id. No. 45，Seq. Id. No. 46，Seq. Id. No. 47，Seq. Id. No. 48， Seq. Id. No. 49，Seq. Id. No. 50，Seq. Id. No. 51，Seq. Id. No. 52，Seq. Id. No. 53，Seq. Id. No. 54，Seq. Id. No. 55，Seq. Id. No. 56，Seq. Id. No. 57，Seq. Id. No. 58，Seq. Id. No. 59， Seq. Id. No. 60，Seq. Id. No. 61，Seq. Id. No. 62，Seq. Id. No. 63，Seq. Id. No. 65，Seq. Id. No. 66，Seq. Id. No. 67，Seq. Id. No. 68，Seq. Id. No. 69，Seq. Id. No. 70，Seq. Id. No. 71， Seq. Id. No. 72，Seq. Id. No. 73，Seq. Id. No. 74，Seq. Id. No. 75，Seq. Id. No. 76，Seq. Id. No. 77, Seq. Id. No. 78, Seq. Id. No. 79, Seq. Id. No. 80, Seq. Id. No. 81，Seq. Id. No. 82， Seq. Id. No. 83，Seq. Id. No. 84，Seq. Id. No. 85，Seq. Id. No. 86，Seq. Id. No. 87，Seq. Id. No. 88，Seq. Id. No. 89，Seq. Id. No. 90，Seq. Id. No. 91，Seq. Id. No. 92，Seq. Id. No. 93， Seq. Id. No. 94，Seq. Id. No. 95，Seq. Id. No. 96，Seq. Id. No. 97，Seq. Id. No. 98，Seq. Id. No. 99, Seq. Id. No. 100, Seq. Id. No. 101, Seq. Id. No. 103, Seq. Id. No. 104, Seq. Id. No. 105, Seq. Id. No. 106, Seq. Id. No. 107, Seq. Id. No. 108, Seq. Id. No. 109, Seq. Id. No. 110, Seq. Id. No. Ill, Seq. Id. No. 112, Seq. Id. No. 113, Seq. Id. No. 114, Seq. Id. No. 115, Seq. Id. No. 116, Seq. Id. No. 117, Seq. Id. No. 118, Seq. Id. No. 119, Seq. Id. No. 120, Seq. Id. No. 121, Seq. Id. No. 122, Seq. Id. No. 123, Seq. Id. No. 124, Seq. Id. No. 125, Seq. Id. No. 126, Seq. Id. No. 127, Seq. Id. No. 128, Seq. Id. No. 129, Seq. Id. No. 130, Seq. Id. No. 131, Seq. Id. No. 132, Seq. Id. No. 133, Seq. Id. No. 134, Seq. Id. No. 135, Seq. Id. No. 136, Seq. Id. No. 137, Seq. Id. No. 138, Seq. Id. No. 139, Seq. Id. No. 140, Seq. Id. No. 141, Seq. Id. No. 142, Seq. Id. No. 143, Seq. Id. No. 144, Seq. Id. No. 145, Seq. Id. No. 146, Seq. Id. No. 147, Seq. Id. No. 148, Seq. Id. No. 149, Seq. Id. No. 150, Seq. Id. No. 151, Seq. Id. No. 152, Seq. Id. No. 153, Seq. Id. No. 154, Seq. Id. No. 155, Seq. Id. No. 156, Seq. Id. No. 157, Seq. Id. No. 158, Seq. Id. No. 159, Seq. Id. No. 160, Seq. Id. No. 161, Seq. Id. No. 162, Seq. Id. No. 163, Seq. Id. No. 164, Seq. Id. No. 165, Seq. Id. No. 166, Seq. Id. No. 167, Seq. Id. No. 168, Seq. Id. No. 169, Seq. Id. No. 170, Seq. Id. No. 171, Seq. Id. No. 172, Seq. Id. No. 173, Seq. Id. No. 174, Seq. Id. No. 175, Seq. Id. No. 176, Seq. Id. No. 177, Seq. Id. No. 178, Seq. Id. No. 179, Seq. Id. No. 180, Seq. Id. No. 181, Seq. Id. No. 182, Seq. Id. No. 183, Seq. Id. No. 184, Seq. Id. No. 185, Seq. Id. No. 186, Seq. Id. No. 187, Seq. Id. No. 188, Seq. Id. No. 189, Seq. Id. No. 190, Seq. Id. No. 191, Seq. Id. No. 192, Seq. Id. No. 193, Seq. Id. No. 194, Seq. Id. No. 195, Seq. Id. No. 196, Seq. Id. No. 197, Seq. Id. No. 198, Seq. Id. No. 199, Seq. Id. No. 200，Seq. Id. No. 201，Seq. Id. No. 202，Seq. Id. No. 203，Seq. Id. No. 204，Seq. Id. No. 205，Seq. Id. No. 206，Seq. Id. No. 207，Seq. Id. No. 208，Seq. Id. No. 209，Seq. Id. No. 210, Seq. Id. No. 211, Seq. Id. No. 212, Seq. Id. No. 213, Seq. Id. No. 214, Seq. Id. No. 215, Seq. Id. No. 216, Seq. Id. No. 217, Seq. Id. No. 218, Seq. Id. No. 219, Seq. Id. No. 220，Seq. Id. No. 221，Seq. Id. No. 222，Seq. Id. No. 223，Seq. Id. No. 224，Seq. Id. No. 225, Seq. Id. No. 226, Seq. Id. No. 227, Seq. Id. No. 228, Seq. Id. No. 229, Seq. Id. No. 230，Seq. Id. No. 231，Seq. Id. No. 232，Seq. Id. No. 233，Seq. Id. No. 234，Seq. Id. No. 235, Seq. Id. No. 236, Seq. Id. No. 237, Seq. Id. No. 238, Seq. Id. No. 239, Seq. Id. No. 240, Seq. Id. No. 241, Seq. Id. No. 242, Seq. Id. No. 243, Seq. Id. No. 244, Seq. Id. No. 245, Seq. Id. No. 246, Seq. Id. No. 247, Seq. Id. No. 248, Seq. Id. No. 249, Seq. Id. No. 250，Seq. Id. No. 251，Seq. Id. No. 252，Seq. Id. No. 253，Seq. Id. No. 254，Seq. Id. No. 255，Seq. Id. No. 256，Seq. Id. No. 257，Seq. Id. No. 258，Seq. Id. No. 259，Seq. Id. No. 260，Seq. Id. No. 261，Seq. Id. No. 262，Seq. Id. No. 263，Seq. Id. No. 264，Seq. Id. No. 265, Seq. Id. No. 266, Seq. Id. No. 267, Seq. Id. No. 268, Seq. Id. No. 269, Seq. Id. No. 270, Seq. Id. No. 271, Seq. Id. No. 272, Seq. Id. No. 273, Seq. Id. No. 274, Seq. Id. No. 275, Seq. Id. No. 276, Seq. Id. No. 277, Seq. Id. No. 278, Seq. Id. No. 279, Seq. Id. No. 280，Seq. Id. No. 281，Seq. Id. No. 282，Seq. Id. No. 283，Seq. Id. No. 284，Seq. Id. No. 285，Seq. Id. No. 286，Seq. Id. No. 287，Seq. Id. No. 288，Seq. Id. No. 289，Seq. Id. No. 290，Seq. Id. No. 291，Seq. Id. No. 292，Seq. Id. No. 293，Seq. Id. No. 294，Seq. Id. No. 295，Seq. Id. No. 296，Seq. Id. No. 297，Seq. Id. No. 298，Seq. Id. No. 299，Seq. Id. No. 300，Seq. Id. No. 301，Seq. Id. No. 302，Seq. Id. No. 303，Seq. Id. No. 304，Seq. Id. No. 305，Seq. Id. No. 306，Seq. Id. No. 307，Seq. Id. No. 308，Seq. Id. No. 310，Seq. Id. No. 311, Seq. Id. No. 312, Seq. Id. No. 313, Seq. Id. No. 314, Seq. Id. No. 315, Seq. Id. No. 316, Seq. Id. No. 317, Seq. Id. No. 318, Seq. Id. No. 319, Seq. Id. No. 320, Seq. Id. No. 321，Seq. Id. No. 322，Seq. Id. No. 323，Seq. Id. No. 324，Seq. Id. No. 325，Seq. Id. No. 326，Seq. Id. No. 327，Seq. Id. No. 328，Seq. Id. No. 329，Seq. Id. No. 330，Seq. Id. No. 331，Seq. Id. No. 332，Seq. Id. No. 333，Seq. Id. No. 334，Seq. Id. No. 335，Seq. Id. No. 336，Seq. Id. No. 337，Seq. Id. No. 338，Seq. Id. No. 339，Seq. Id. No. 340，Seq. Id. No. 341，Seq. Id. No. 342, Seq. Id. No. 343, Seq. Id. No. 344, Seq. Id. No. 345, Seq. Id. No. 346, Seq. Id. No. 347, Seq. Id. No. 348, Seq. Id. No. 349, Seq. Id. No. 350, Seq. Id. No. 351, Seq. Id. No. 352, Seq. Id. No. 353, Seq. Id. No. 354, Seq. Id. No. 355, Scq. Id. No. 356, Seq. Id. No. 357, Seq. Id. No. 358, Seq. Id. No. 359, Seq. Id. No. 360, Seq. Id. No. 361, Seq. Id. No. 362, Seq. Id. No. 363, Seq. Id. No. 364, Seq. Id. No. 365, Seq. Id. No. 366, Seq. Id. No. 367, Seq. Id. No. 368, Seq. Id. No. 369, Seq. Id. No. 371, Seq. Id. No. 372, Seq. Id. No. 374, Seq. Id. No. 375, Seq. Id. No. 376, Seq. Id. No. 377, Seq. Id. No. 378, Seq. Id. No. 379, Seq. Id. No. 380, Seq. Id. No. 381, Seq. Id. No. 382, Seq. Id. No. 383, Seq. Id. No. 384, Seq. Id. No. 385, Seq. Id. No. 386, Seq. Id. No. 387, Seq. Id. No. 388, Seq. Id. No. 389, Seq. Id. No. 390, Seq. Id. No. 391, Seq. Id. No. 392, Seq. Id. No. 393, Seq. Id. No. 394, Seq. Id. No. 395, Seq. Id. No. 396, Seq. Id. No. 397, Seq. Id. No. 398, Seq. Id. No. 399, Seq. Id. No. 400, Seq. Id. No. 401, Seq. Id. No. 402, Seq. Id. No. 403, Seq. Id. No. 404, Seq. Id. No. 405, Seq. Id. No. 406, Seq. Id. No. 407, Seq. Id. No. 408, Seq. Id. No. 409, Seq. Id. No. 410, Seq. Id. No. 411, Seq. Id. No. 412, Seq. Id. No. 413, Seq. Id. No. 414, Seq. Id. No. 415, Seq. Id. No. 416, Seq. Id. No. 417, Seq. Id. No. 418, Seq. Id. No. 419, Seq. Id. No. 420, Seq. Id. No. 421, Seq. Id. No. 422, Seq. Id. No. 423, Seq. Id. No. 424, Seq. Id. No. 425, Seq. Id. No. 426, Seq. Id. No. 427, Seq. Id. No. 428, Seq. Id. No. 429, Seq. Id. No. 430, Seq. Id. No. 431, Seq. Id. No. 432, Seq. Id. No. 433, Seq. Id. No. 434, Seq. Id. No. 435, Seq. Id. No. 436, Seq. Id. No. 437, Seq. Id. No. 438, Seq. Id. No. 439, Seq. Id. No. 440, Seq. Id. No. 441, Seq. Id. No. 442, Seq. Id. No. 443, Seq. Id. No. 444, Seq. Id. No. 445, Seq. Id. No. 446, Seq. Id. No. 447, Seq. Id. No. 448, Seq. Id. No. 449, Seq. Id. No. 450, Seq. Id. No. 451, Seq. Id. No. 452, Seq. Id. No. 453, Seq. Id. No. 454, Seq. Id. No. 455, Seq. Id. No. 456, Seq. Id. No. 457, Seq. Id. No. 458, Seq. Id. No. 459, Seq. Id. No. 460, Seq. Id. No. 461, Seq. Id. No. 462, Seq. Id. No. 463, Seq. Id. No. 464, Seq. Id. No. 465, Seq. Id. No. 466, Seq. Id. No. 467, Seq. Id. No. 468, Seq. Id. No. 469, Seq. Id. No. 470, Seq. Id. No. 471, Seq. Id. No. 472, Seq. Id. No. 473, Seq. Id. No. 474, Seq. Id. No. 475, Seq. Id. No. 476, Seq. Id. No. 477, Seq. Id. No. 478, Seq. Id. No. 479, Seq. Id. No. 480, Seq. Id. No. 481, Seq. Id. No. 482, Seq. Id. No. 483, Seq. Id. No. 484, Seq. Id. No. 485, Seq. Id. No. 486, Seq. Id. No. 487, Seq. Id. No. 488, Seq. Id. No. 489, Seq. Id. No. 490, Seq. Id. No. 491, Seq. Id. No. 492, Seq. Id. No. 493, Seq. Id. No. 494, Seq. Id. No. 495, Seq. Id. No. 496, Seq. Id. No. 497, Seq. Id. No. 498, Seq. Id. No. 499, Seq. Id. No. 500, Seq. Id. No. 501, Seq. Id. No. 502, Seq. Id. No. 503, Seq. Id. No. 504, Seq. Id. No. 505, Seq. Id. No. 506, Seq. Id. No. 507, Seq. Id. No. 508, Seq. Id. No. 509, Seq. Id. No. 510, Seq. Id. No. 511, Seq. Id. No. 512, Seq. Id. No. 513, Seq. Id. No. 514, Seq. Id. No. 515, Seq. Id. No. 516 和 Seq. Id. No. 517。
6.依照权利要求1-3任一项的方法，其中所述自身抗体识别选自下组的突变型EGFR 肽： Seq. Id. No. 518, Seq. Id. No. 522, Seq. Id. No. 523, Seq. Id. No. 524, Seq. Id. No. 526, Seq. Id. No. 527，Seq. Id. No. 528，Seq. Id. No. 529，Seq. Id. No. 530，Seq. Id. No. 531，Seq. Id. No. 532，Seq. Id. No. 533，Seq. Id. No. 534，Seq. Id. No. 535，Seq. Id. No. 536，Seq. Id. No. 537, Seq. Id. No. 538, Seq. Id. No. 539, Seq. Id. No. 540, Seq. Id. No. 541, Seq. Id. No. 542, Seq. Id. No. 543, Seq. Id. No. 544, Seq. Id. No. 545, Seq. Id. No. 546, Seq. Id. No. 547, Seq. Id. No. 548, Seq. Id. No. 549, Seq. Id. No. 550, Seq. Id. No. 551, Seq. Id. No. 552，Seq. Id. No. 553，Seq. Id. No. 554，Seq. Id. No. 555，Seq. Id. No. 557，Seq. Id. No. 559，Seq. Id. No. 560，Seq. Id. No. 562，Seq. Id. No. 563，Seq. Id. No. 564，Seq. Id. No. 565, Seq. Id. No. 567, Seq. Id. No. 568, Seq. Id. No. 569, Seq. Id. No. 570, Seq. Id. No. 571, Seq. Id. No. 572, Seq. Id. No. 573, Seq. Id. No. 574, Seq. Id. No. 575, Seq. Id. No. 576, Seq. Id. No. 577, Seq. Id. No. 578, Seq. Id. No. 579, Seq. Id. No. 580, Seq. Id. No. 581，Seq. Id. No. 582，Seq. Id. No. 583，Seq. Id. No. 584，Seq. Id. No. 585，Seq. Id. No. 586，Seq. Id. No. 587，Seq. Id. No. 588，Seq. Id. No. 589，Seq. Id. No. 590，Seq. Id. No. 591，Seq. Id. No. 592，Seq. Id. No. 593，Seq. Id. No. 594，Seq. Id. No. 595，Seq. Id. No. 596, Seq. Id. No. 597, Seq. Id. No. 598, Seq. Id. No. 599 和 Seq. Id. No. 601。
7. 依照权利要求1-3任一项的方法，其中所述自身抗体识别选自下组的突变型EGFR 肽：Seq. Id. No. 604, Seq. Id. No. 605, Seq. Id. No. 606, Seq. Id. No. 607, Seq. Id. No. 608， Seq. Id. No. 609, Seq. Id. No. 610, Seq. Id. No. 611, Seq. Id. No. 612, Seq. Id. No. 613, Seq. Id. No. 614, Seq. Id. No. 615, Seq. Id. No. 616, Seq. Id. No. 617, Seq. Id. No. 618 和 Seq. Id. No. 619。
8. 依照权利要求1-7任一项的方法，其中该人受试者的血液样品中自身抗体的水平比 代表健康群体人受试者的所述自身抗体的水平高5倍。
9. 厄洛替尼（Erlotinib)或其药学可接受盐，特别是厄洛替尼盐酸盐，其用于治疗通 过权利要求1至8任一项的方法鉴定的NSCLC患者，包括对该患者施用厄洛替尼或其药学 可接受盐，特别是厄洛替尼盐酸盐。
10. 自身抗体用于预测NSCLC患者对厄洛替尼或其药学可接受盐，特别是厄洛替尼盐 酸盐，治疗的响应的用途，该抗体是通过权利要求1至8任一项的方法鉴定的。
11. 一种用于在人受试者的血液样品中检测一种或多种选自识别突变型人EGFR的自 身抗体组的自身抗体的水平的试剂盒，其中该人受试者的血液样品中所述选自识别突变型 人EGFR的自身抗体组的自身抗体的水平与代表健康群体人受试者的所述自身抗体的水平 相比升高指示非小细胞肺癌。
12. 依照权利要求11的试剂盒，其中所述自身抗体识别选自下组的突变型EGFR肽： Seq. Id. No. 554, Seq. Id. No. 555, Seq. Id. No. 556, Seq. Id. No. 557, Seq. Id. No. 558, Seq. Id. No. 559,和 Seq. Id. No. 560。
13. 依照权利要求11的试剂盒，其中所述自身抗体识别选自下组的突变型EGFR肽： Seq. Id. No. 1，Seq. Id. No. 2，Seq. Id. No. 4，Seq. Id. No. 5，Seq. Id. No. 6，Seq. Id. No. 7 和 Seq. Id. No. 15。
14. 依照权利要求11的试剂盒，其中所述自身抗体识别选自下组的突变型EGFR肽： Seq. Id. No. 16, Seq. Id. No. 17, Seq. Id. No. 18, Seq. Id. No. 19, Seq. Id. No. 20, Seq. Id. No. 21, Seq. Id. No. 22, Seq. Id. No. 23, Seq. Id. No. 24, Seq. Id. No. 25, Seq. Id. No. 26, Seq. Id. No. 27, Seq. Id. No. 28, Seq. Id. No. 29, Seq. Id. No. 30, Seq. Id. No. 31, Seq. Id. No. 32, Seq. Id. No. 33, Seq. Id. No. 34, Seq. Id. No. 35, Seq. Id. No. 36, Seq. Id. No. 37, Seq. Id. No. 38, Seq. Id. No. 39, Seq. Id. No. 40, Seq. Id. No. 41, Seq. Id. No. 42, Seq. Id. No. 43, Seq. Id. No. 44, Seq. Id. No. 45, Seq. Id. No. 46, Seq. Id. No. 47, Seq. Id. No. 48, Seq. Id. No. 49, Seq. Id. No. 50, Seq. Id. No. 51, Seq. Id. No. 52, Seq. Id. No. 53, Seq. Id. No. 54, Seq. Id. No. 55, Seq. Id. No. 56, Seq. Id. No. 57, Seq. Id. No. 58, Seq. Id. No. 59, Seq. Id. No. 60, Seq. Id. No. 61, Seq. Id. No. 62, Seq. Id. No. 63, Seq. Id. No. 65, Seq. Id. No. 66, Seq. Id. No. 67, Seq. Id. No. 68, Seq. Id. No. 69, Seq. Id. No. 70, Seq. Id. No. 71, Seq. Id. No. 72, Seq. Id. No. 73, Seq. Id. No. 74, Seq. Id. No. 75, Seq. Id. No. 76, Seq. Id. No. 77, Seq. Id. No. 78, Seq. Id. No. 79, Seq. Id. No. 80, Seq. Id. No. 81, Seq. Id. No. 82, Seq. Id. No. 83, Seq. Id. No. 84, Seq. Id. No. 85, Seq. Id. No. 86, Seq. Id. No. 87, Seq. Id. No. 88, Seq. Id. No. 89, Seq. Id. No. 90, Seq. Id. No. 91, Seq. Id. No. 92, Seq. Id. No. 93, Seq. Id. No. 94, Seq. Id. No. 95, Seq. Id. No. 96, Seq. Id. No. 97, Seq. Id. No. 98, Seq. Id. No. 99, Seq. Id. No. 100, Seq. Id. No. 101, Seq. Id. No. 103, Seq. Id. No. 104, Seq. Id. No. 105, Seq. Id. No. 106, Seq. Id. No. 107, Seq. Id. No. 108, Seq. Id. No. 109, Seq. Id. No. 110, Seq. Id. No. Ill, Seq. Id. No. 112, Seq. Id. No. 113, Seq. Id. No. 114, Seq. Id. No. 115, Seq. Id. No. 116, Seq. Id. No. 117, Seq. Id. No. 118, Seq. Id. No. 119, Seq. Id. No. 120, Seq. Id. No. 121, Seq. Id. No. 122, Seq. Id. No. 123, Seq. Id. No. 124, Seq. Id. No. 125, Seq. Id. No. 126, Seq. Id. No. 127, Seq. Id. No. 128, Seq. Id. No. 129, Seq. Id. No. 130, Seq. Id. No. 131, Seq. Id. No. 132, Seq. Id. No. 133, Seq. Id. No. 134, Seq. Id. No. 135, Seq. Id. No. 136, Seq. Id. No. 137, Seq. Id. No. 138, Seq. Id. No. 139, Seq. Id. No. 140, Seq. Id. No. 141, Seq. Id. No. 142, Seq. Id. No. 143, Seq. Id. No. 144, Seq. Id. No. 145, Seq. Id. No. 146, Seq. Id. No. 147, Seq. Id. No. 148, Seq. Id. No. 149, Seq. Id. No. 150, Seq. Id. No. 151, Seq. Id. No. 152, Seq. Id. No. 153, Seq. Id. No. 154, Seq. Id. No. 155, Seq. Id. No. 156, Seq. Id. No. 157, Seq. Id. No. 158, Seq. Id. No. 159, Seq. Id. No. 160, Seq. Id. No. 161, Seq. Id. No. 162, Seq. Id. No. 163, Seq. Id. No. 164, Seq. Id. No. 165, Seq. Id. No. 166, Seq. Id. No. 167, Seq. Id. No. 168, Seq. Id. No. 169, Seq. Id. No. 170, Seq. Id. No. 171, Seq. Id. No. 172, Seq. Id. No. 173, Seq. Id. No. 174, Seq. Id. No. 175, Seq. Id. No. 176, Seq. Id. No. 177, Seq. Id. No. 178, Seq. Id. No. 179, Seq. Id. No. 180, Seq. Id. No. 181, Seq. Id. No. 182, Seq. Id. No. 183, Seq. Id. No. 184, Seq. Id. No. 185, Seq. Id. No. 186, Seq. Id. No. 187, Seq. Id. No. 188, Seq. Id. No. 189, Seq. Id. No. 190, Seq. Id. No. 191, Seq. Id. No. 192, Seq. Id. No. 193, Seq. Id. No. 194, Seq. Id. No. 195, Seq. Id. No. 196, Seq. Id. No. 197, Seq. Id. No. 198, Seq. Id. No. 199, Seq. Id. No. 200, Seq. Id. No. 201, Seq. Id. No. 202, Seq. Id. No. 203, Seq. Id. No. 204, Seq. Id. No. 205, Seq. Id. No. 206, Seq. Id. No. 207, Seq. Id. No. 208, Seq. Id. No. 209, Seq. Id. No. 210, Seq. Id. No. 211, Seq. Id. No. 212, Seq. Id. No. 213, Seq. Id. No. 214, Seq. Id. No. 215, Seq. Id. No. 216, Seq. Id. No. 217, Seq. Id. No. 218, Seq. Id. No. 219, Seq. Id. No. 220, Seq. Id. No. 221, Seq. Id. No. 222, Seq. Id. No. 223, Seq. Id. No. 224, Seq. Id. No. 225, Seq. Id. No. 226, Seq. Id. No. 227, Seq. Id. No. 228, Seq. Id. No. 229, Seq. Id. No. 230, Seq. Id. No. 231, Seq. Id. No. 232, Seq. Id. No. 233, Seq. Id. No. 234, Seq. Id. No. 235, Seq. Id. No. 236, Seq. Id. No. 237, Seq. Id. No. 238, Seq. Id. No. 239, Seq. Id. No. 240, Seq. Id. No. 241, Seq. Id. No. 242, Seq. Id. No. 243, Seq. Id. No. 244, Seq. Id. No. 245, Seq. Id. No. 246, Seq. Id. No. 247, Seq. Id. No. 248, Seq. Id. No. 249, Seq. Id. No. 250，Seq. Id. No. 251，Seq. Id. No. 252，Seq. Id. No. 253，Seq. Id. No. 254，Seq. Id. No. 255，Seq. Id. No. 256，Seq. Id. No. 257，Seq. Id. No. 258，Seq. Id. No. 259，Seq. Id. No. 260，Seq. Id. No. 261，Seq. Id. No. 262，Seq. Id. No. 263，Seq. Id. No. 264，Seq. Id. No. 265, Seq. Id. No. 266, Seq. Id. No. 267, Seq. Id. No. 268, Seq. Id. No. 269, Seq. Id. No. 270, Seq. Id. No. 271, Seq. Id. No. 272, Seq. Id. No. 273, Seq. Id. No. 274, Seq. Id. No. 275, Seq. Id. No. 276, Seq. Id. No. 277, Seq. Id. No. 278, Seq. Id. No. 279, Seq. Id. No. 280，Seq. Id. No. 281，Seq. Id. No. 282，Seq. Id. No. 283，Seq. Id. No. 284，Seq. Id. No. 285，Seq. Id. No. 286，Seq. Id. No. 287，Seq. Id. No. 288，Seq. Id. No. 289，Seq. Id. No. 290，Seq. Id. No. 291，Seq. Id. No. 292，Seq. Id. No. 293，Seq. Id. No. 294，Seq. Id. No. 295，Seq. Id. No. 296，Seq. Id. No. 297，Seq. Id. No. 298，Seq. Id. No. 299，Seq. Id. No. 300，Seq. Id. No. 301，Seq. Id. No. 302，Seq. Id. No. 303，Seq. Id. No. 304，Seq. Id. No. 305，Seq. Id. No. 306，Seq. Id. No. 307，Seq. Id. No. 308，Seq. Id. No. 310，Seq. Id. No. 311, Seq. Id. No. 312, Seq. Id. No. 313, Seq. Id. No. 314, Seq. Id. No. 315, Seq. Id. No. 316, Seq. Id. No. 317, Seq. Id. No. 318, Seq. Id. No. 319, Seq. Id. No. 320, Seq. Id. No. 321，Seq. Id. No. 322，Seq. Id. No. 323，Seq. Id. No. 324，Seq. Id. No. 325，Seq. Id. No. 326，Seq. Id. No. 327，Seq. Id. No. 328，Seq. Id. No. 329，Seq. Id. No. 330，Seq. Id. No. 331，Seq. Id. No. 332，Seq. Id. No. 333，Seq. Id. No. 334，Seq. Id. No. 335，Seq. Id. No. 336，Seq. Id. No. 337，Seq. Id. No. 338，Seq. Id. No. 339，Seq. Id. No. 340，Seq. Id. No. 341, Seq. Id. No. 342, Seq. Id. No. 343, Seq. Id. No. 344, Seq. Id. No. 345, Seq. Id. No. 346, Seq. Id. No. 347, Seq. Id. No. 348, Seq. Id. No. 349, Seq. Id. No. 350, Seq. Id. No. 351，Seq. Id. No. 352，Seq. Id. No. 353，Seq. Id. No. 354，Seq. Id. No. 355，Seq. Id. No. 356，Seq. Id. No. 357，Seq. Id. No. 358，Seq. Id. No. 359，Seq. Id. No. 360，Seq. Id. No. 361，Seq. Id. No. 362，Seq. Id. No. 363，Seq. Id. No. 364，Seq. Id. No. 365，Seq. Id. No. 366, Seq. Id. No. 367, Seq. Id. No. 368, Seq. Id. No. 369, Seq. Id. No. 371, Seq. Id. No. 372, Seq. Id. No. 374, Seq. Id. No. 375, Seq. Id. No. 376, Seq. Id. No. 377, Seq. Id. No. 378, Seq. Id. No. 379, Seq. Id. No. 380, Seq. Id. No. 381, Seq. Id. No. 382, Seq. Id. No. 383，Seq. Id. No. 384，Seq. Id. No. 385，Seq. Id. No. 386，Seq. Id. No. 387，Seq. Id. No. 388，Seq. Id. No. 389，Seq. Id. No. 390，Seq. Id. No. 391，Seq. Id. No. 392，Seq. Id. No. 393，Seq. Id. No. 394，Seq. Id. No. 395，Seq. Id. No. 396，Seq. Id. No. 397，Seq. Id. No. 398, Seq. Id. No. 399, Seq. Id. No. 400, Seq. Id. No. 401, Seq. Id. No. 402, Seq. Id. No. 403, Seq. Id. No. 404, Seq. Id. No. 405, Seq. Id. No. 406, Seq. Id. No. 407, Seq. Id. No. 408, Seq. Id. No. 409, Seq. Id. No. 410, Seq. Id. No. 411, Seq. Id. No. 412, Seq. Id. No. 413, Seq. Id. No. 414, Seq. Id. No. 415, Seq. Id. No. 416, Seq. Id. No. 417, Seq. Id. No. 418, Seq. Id. No. 419, Seq. Id. No. 420, Seq. Id. No. 421, Seq. Id. No. 422, Seq. Id. No. 423, Seq. Id. No. 424, Seq. Id. No. 425, Seq. Id. No. 426, Seq. Id. No. 427, Seq. Id. No. 428, Seq. Id. No. 429, Seq. Id. No. 430, Seq. Id. No. 431, Seq. Id. No. 432, Seq. Id. No. 433, Seq. Id. No. 434, Seq. Id. No. 435, Seq. Id. No. 436, Seq. Id. No. 437, Seq. Id. No. 438, Seq. Id. No. 439, Seq. Id. No. 440, Seq. Id. No. 441, Seq. Id. No. 442, Seq. Id. No. 443, Seq. Id. No. 444, Seq. Id. No. 445, Seq. Id. No. 446, Seq. Id. No. 447, Seq. Id. No. 448, Seq. Id. No. 449, Seq. Id. No. 450, Seq. Id. No. 451, Seq. Id. No. 452, Seq. Id. No. 453, Seq. Id. No. 454, Seq. Id. No. 455, Seq. Id. No. 456, Seq. Id. No. 457, Seq. Id. No. 458, Seq. Id. No. 459, Seq. Id. No. 460, Seq. Id. No. 461, Seq. Id. No. 462, Seq. Id. No. 463, Seq. Id. No. 464, Seq. Id. No. 465, Seq. Id. No. 466, Seq. Id. No. 467, Seq. Id. No. 468, Seq. Id. No. 469, Seq. Id. No. 470, Seq. Id. No. 471, Seq. Id. No. 472, Seq. Id. No. 473, Seq. Id. No. 474, Seq. Id. No. 475, Seq. Id. No. 476, Seq. Id. No. 477, Seq. Id. No. 478, Seq. Id. No. 479, Seq. Id. No. 480, Seq. Id. No. 481, Seq. Id. No. 482, Seq. Id. No. 483, Seq. Id. No. 484, Seq. Id. No. 485, Seq. Id. No. 486, Seq. Id. No. 487, Seq. Id. No. 488, Seq. Id. No. 489, Seq. Id. No. 490, Seq. Id. No. 491, Seq. Id. No. 492, Seq. Id. No. 493, Seq. Id. No. 494, Seq. Id. No. 495, Seq. Id. No. 496, Seq. Id. No. 497, Seq. Id. No. 498, Seq. Id. No. 499, Seq. Id. No. 500, Seq. Id. No. 501, Seq. Id. No. 502, Seq. Id. No. 503, Seq. Id. No. 504, Seq. Id. No. 505, Seq. Id. No. 506, Seq. Id. No. 507, Seq. Id. No. 508, Seq. Id. No. 509, Seq. Id. No. 510, Seq. Id. No. 511, Seq. Id. No. 512, Seq. Id. No. 513, Seq. Id. No. 514, Seq. Id. No. 515, Seq. Id. No. 516 和 Seq. Id. No. 517。
15. 依照权利要求11的试剂盒，其中所述自身抗体识别选自下组的突变型EGFR肽： Seq. Id. No. 518, Seq. Id. No. 522, Seq. Id. No. 523, Seq. Id. No. 524, Seq. Id. No. 526, Seq. Id. No. 527，Seq. Id. No. 528，Seq. Id. No. 529，Seq. Id. No. 530，Seq. Id. No. 531，Seq. Id. No. 532，Seq. Id. No. 533，Seq. Id. No. 534，Seq. Id. No. 535，Seq. Id. No. 536，Seq. Id. No. 537, Seq. Id. No. 538, Seq. Id. No. 539, Seq. Id. No. 540, Seq. Id. No. 541, Seq. Id. No. 542, Seq. Id. No. 543, Seq. Id. No. 544, Seq. Id. No. 545, Seq. Id. No. 546, Seq. Id. No. 547, Seq. Id. No. 548, Seq. Id. No. 549, Seq. Id. No. 550, Seq. Id. No. 551, Seq. Id. No. 552，Seq. Id. No. 553，Seq. Id. No. 554，Seq. Id. No. 555，Seq. Id. No. 557，Seq. Id. No. 559，Seq. Id. No. 560，Seq. Id. No. 562，Seq. Id. No. 563，Seq. Id. No. 564，Seq. Id. No. 565, Seq. Id. No. 567, Seq. Id. No. 568, Seq. Id. No. 569, Seq. Id. No. 570, Seq. Id. No. 571, Seq. Id. No. 572, Seq. Id. No. 573, Seq. Id. No. 574, Seq. Id. No. 575, Seq. Id. No. 576, Seq. Id. No. 577, Seq. Id. No. 578, Seq. Id. No. 579, Seq. Id. No. 580, Seq. Id. No. 581，Seq. Id. No. 582，Seq. Id. No. 583，Seq. Id. No. 584，Seq. Id. No. 585，Seq. Id. No. 586，Seq. Id. No. 587，Seq. Id. No. 588，Seq. Id. No. 589，Seq. Id. No. 590，Seq. Id. No. 591，Seq. Id. No. 592，Seq. Id. No. 593，Seq. Id. No. 594，Seq. Id. No. 595，Seq. Id. No. 596, Seq. Id. No. 597, Seq. Id. No. 598, Seq. Id. No. 599 和 Seq. Id. No. 601。
16. 依照权利要求11的试剂盒，其中所述自身抗体识别选自下组的突变型EGFR 肽：Seq. Id. No. 604, Seq. Id. No. 605, Seq. Id. No. 606, Seq. Id. No. 607, Seq. Id. No. 608， Seq. Id. No. 609, Seq. Id. No. 610, Seq. Id. No. 611, Seq. Id. No. 612, Seq. Id. No. 613, Seq. Id. No. 614, Seq. Id. No. 615, Seq. Id. No. 616, Seq. Id. No. 617, Seq. Id. No. 618 和 Seq. Id. No. 619。
17. -种在人受试者中诊断非小细胞肺癌的方法，其包括： 在该人受试者的血液样品中测量选自识别人EGFR的自身抗体组的自身抗体的水平， 其中该人受试者的血液样品中所述选自识别人EGFR的自身抗体组的自身抗体的水平与代 表健康群体人受试者的所述自身抗体的水平相比升高指示非小细胞肺癌。
18. 依照权利要求16的方法，其中所述自身抗体识别选自下组的突变型EGFR肽：Seq. Id. No. 519, Seq. Id. No. 520, Seq. Id. No. 521 和 Seq. Id. No. 561。
19. 依照权利要求17-18任一项的方法，其中所述自身抗体识别选自下组的EGFR肽： Seq. Id. No. 1, Seq. Id. No. 2, Seq. Id. No. 3, Seq. Id. No. 4, Seq. Id. No. 5, Seq. Id. No. 6, Seq. Id. No. 7，Seq. Id. No. 8，Seq. Id. No. 9，Seq. Id. No. 10，Seq. Id. No. 11，Seq. Id. No. 12， Seq. Id. No. 13，Seq. Id. No. 14，Seq. Id. No. 15，Seq. Id. No. 16，Seq. Id. No. 17，Seq. Id. No. 18，Seq. Id. No. 19，Seq. Id. No. 20，Seq. Id. No. 21，Seq. Id. No. 22，Seq. Id. No. 23，Seq. Id. No. 24，Seq. Id. No. 25，Seq. Id. No. 26，Seq. Id. No. 27，Seq. Id. No. 28，Seq. Id. No. 29， Seq. Id. No. 30，Seq. Id. No. 31，Seq. Id. No. 32，Seq. Id. No. 33，Seq. Id. No. 34，Seq. Id. No. 35，Seq. Id. No. 36，Seq. Id. No. 37，Seq. Id. No. 38，Seq. Id. No. 39，Seq. Id. No. 40，Seq. Id. No. 41，Seq. Id. No. 42，Seq. Id. No. 43，Seq. Id. No. 44，Seq. Id. No. 45，Seq. Id. No. 46， Seq. Id. No. 47，Seq. Id. No. 48，Seq. Id. No. 49，Seq. Id. No. 50，Seq. Id. No. 51，Seq. Id. No. 52，Seq. Id. No. 53，Seq. Id. No. 54，Seq. Id. No. 55，Seq. Id. No. 56，Seq. Id. No. 57，Seq. Id. No. 58，Seq. Id. No. 59，Seq. Id. No. 60，Seq. Id. No. 61，Seq. Id. No. 62，Seq. Id. No. 63， Seq. Id. No. 64，Seq. Id. No. 65，Seq. Id. No. 66，Seq. Id. No. 67，Seq. Id. No. 68，Seq. Id. No. 69，Seq. Id. No. 70，Seq. Id. No. 71，Seq. Id. No. 72，Seq. Id. No. 73，Seq. Id. No. 74，Seq. Id. No. 75, Seq. Id. No. 76, Seq. Id. No. 77, Seq. Id. No. 78, Seq. Id. No. 79, Seq. Id. No. 80， Seq. Id. No. 81，Seq. Id. No. 82，Seq. Id. No. 83，Seq. Id. No. 84，Seq. Id. No. 85，Seq. Id. No. 86，Seq. Id. No. 87，Seq. Id. No. 88，Seq. Id. No. 89，Seq. Id. No. 90，Seq. Id. No. 91，Seq. Id. No. 92，Seq. Id. No. 93，Seq. Id. No. 94，Seq. Id. No. 95，Seq. Id. No. 96，Seq. Id. No. 97， Seq. Id. No. 98，Seq. Id. No. 99，Seq. Id. No. 100，Seq. Id. No. 101，Seq. Id. No. 102，Seq. Id. No. 103, Seq. Id. No. 104, Seq. Id. No. 105, Seq. Id. No. 106, Seq. Id. No. 107, Seq. Id. No. 108, Seq. Id. No. 109, Seq. Id. No. 110, Seq. Id. No. Ill, Seq. Id. No. 112, Seq. Id. No. 113, Seq. Id. No. 114, Seq. Id. No. 115, Seq. Id. No. 116, Seq. Id. No. 117, Seq. Id. No. 118, Seq. Id. No. 119, Seq. Id. No. 120, Seq. Id. No. 121, Seq. Id. No. 122, Seq. Id. No. 123, Seq. Id. No. 124, Seq. Id. No. 125, Seq. Id. No. 126, Seq. Id. No. 127, Seq. Id. No. 128, Seq. Id. No. 129, Seq. Id. No. 130, Seq. Id. No. 131, Seq. Id. No. 132, Seq. Id. No. 133, Seq. Id. No. 134, Seq. Id. No. 135, Seq. Id. No. 136, Seq. Id. No. 137, Seq. Id. No. 138, Seq. Id. No. 139, Seq. Id. No. 140, Seq. Id. No. 141, Seq. Id. No. 142, Seq. Id. No. 143, Seq. Id. No. 144, Seq. Id. No. 145, Seq. Id. No. 146, Seq. Id. No. 147, Seq. Id. No. 148, Seq. Id. No. 149, Seq. Id. No. 150, Seq. Id. No. 151, Seq. Id. No. 152, Seq. Id. No. 153, Seq. Id. No. 154, Seq. Id. No. 155, Seq. Id. No. 156, Seq. Id. No. 157, Seq. Id. No. 158, Seq. Id. No. 159, Seq. Id. No. 160, Seq. Id. No. 161, Seq. Id. No. 162, Seq. Id. No. 163, Seq. Id. No. 164, Seq. Id. No. 165, Seq. Id. No. 166, Seq. Id. No. 167, Seq. Id. No. 168, Seq. Id. No. 169, Seq. Id. No. 170, Seq. Id. No. 171, Seq. Id. No. 172, Seq. Id. No. 173, Seq. Id. No. 174, Seq. Id. No. 175, Seq. Id. No. 176, Seq. Id. No. 177, Seq. Id. No. 178, Seq. Id. No. 179, Seq. Id. No. 180, Seq. Id. No. 181, Seq. Id. No. 182, Seq. Id. No. 183, Seq. Id. No. 184, Seq. Id. No. 185, Seq. Id. No. 186, Seq. Id. No. 187, Seq. Id. No. 188, Seq. Id. No. 189, Seq. Id. No. 190, Seq. Id. No. 191, Seq. Id. No. 192, Seq. Id. No. 193, Seq. Id. No. 194, Seq. Id. No. 195, Seq. Id. No. 196, Seq. Id. No. 197, Seq. Id. No. 198, Seq. Id. No. 199, Seq. Id. No. 200, Seq. Id. No. 201, Seq. Id. No. 202, Seq. Id. No. 203, Seq. Id. No. 204, Seq. Id. No. 205, Seq. Id. No. 206, Seq. Id. No. 207, Seq. Id. No. 208, Seq. Id. No. 209, Seq. Id. No. 210, Seq. Id. No. 211, Seq. Id. No. 212, Seq. Id. No. 213, Seq. Id. No. 214, Seq. Id. No. 215, Seq. Id. No. 216, Seq. Id. No. 217, Seq. Id. No. 218, Seq. Id. No. 219, Seq. Id. No. 220, Seq. Id. No. 221, Seq. Id. No. 222, Seq. Id. No. 223, Seq. Id. No. 224, Seq. Id. No. 225, Seq. Id. No. 226, Seq. Id. No. 227, Seq. Id. No. 228, Seq. Id. No. 229, Seq. Id. No. 230, Seq. Id. No. 231, Seq. Id. No. 232, Seq. Id. No. 233, Seq. Id. No. 234, Seq. Id. No. 235, Seq. Id. No. 236, Seq. Id. No. 237, Seq. Id. No. 238, Seq. Id. No. 239, Seq. Id. No. 240, Seq. Id. No. 241, Seq. Id. No. 242, Seq. Id. No. 243, Seq. Id. No. 244, Seq. Id. No. 245, Seq. Id. No. 246, Seq. Id. No. 247, Seq. Id. No. 248, Seq. Id. No. 249, Seq. Id. No. 250, Seq. Id. No. 251, Seq. Id. No. 252, Seq. Id. No. 253, Seq. Id. No. 254, Seq. Id. No. 255, Seq. Id. No. 256, Seq. Id. No. 257, Seq. Id. No. 258, Seq. Id. No. 259, Seq. Id. No. 260, Seq. Id. No. 261, Seq. Id. No. 262, Seq. Id. No. 263, Seq. Id. No. 264, Seq. Id. No. 265, Seq. Id. No. 266, Seq. Id. No. 267, Seq. Id. No. 268, Seq. Id. No. 269, Seq. Id. No. 270, Seq. Id. No. 271, Seq. Id. No. 272, Seq. Id. No. 273, Seq. Id. No. 274, Seq. Id. No. 275, Seq. Id. No. 276, Seq. Id. No. 277, Seq. Id. No. 278, Seq. Id. No. 279, Seq. Id. No. 280, Seq. Id. No. 281, Seq. Id. No. 282, Seq. Id. No. 283, Seq. Id. No. 284, Seq. Id. No. 285, Seq. Id. No. 286, Seq. Id. No. 287, Seq. Id. No. 288, Seq. Id. No. 289, Seq. Id. No. 290, Seq. Id. No. 291, Seq. Id. No. 292, Seq. Id. No. 293, Seq. Id. No. 294, Seq. Id. No. 295, Seq. Id. No. 296, Seq. Id. No. 297, Seq. Id. No. 298, Seq. Id. No. 299, Seq. Id. No. 300, Seq. Id. No. 301, Seq. Id. No. 302, Seq. Id. No. 303, Seq. Id. No. 304, Seq. Id. No. 305, Seq. Id. No. 306, Seq. Id. No. 307, Seq. Id. No. 308, Seq. Id. No. 309, Seq. Id. No. 310, Seq. Id. No. 311, Seq. Id. No. 312, Seq. Id. No. 313, Seq. Id. No. 314, Seq. Id. No. 315, Seq. Id. No. 316, Seq. Id. No. 317, Seq. Id. No. 318, Seq. Id. No. 319, Seq. Id. No. 320, Seq. Id. No. 321, Seq. Id. No. 322, Seq. Id. No. 323, Seq. Id. No. 324, Seq. Id. No. 325, Seq. Id. No. 326, Seq. Id. No. 327, Seq. Id. No. 328, Seq. Id. No. 329, Seq. Id. No. 330, Seq. Id. No. 331, Seq. Id. No. 332, Seq. Id. No. 333, Seq. Id. No. 334, Seq. Id. No. 335, Seq. Id. No. 336, Seq. Id. No. 337, Seq. Id. No. 338, Seq. Id. No. 339, Seq. Id. No. 340, Seq. Id. No. 341, Seq. Id. No. 342, Seq. Id. No. 343, Seq. Id. No. 344, Seq. Id. No. 345, Seq. Id. No. 346, Seq. Id. No. 347, Seq. Id. No. 348, Seq. Id. No. 349, Seq. Id. No. 350, Seq. Id. No. 351, Seq. Id. No. 352, Seq. Id. No. 353, Seq. Id. No. 354, Seq. Id. No. 355, Seq. Id. No. 356, Seq. Id. No. 357, Seq. Id. No. 358, Seq. Id. No. 359, Seq. Id. No. 360, Seq. Id. No. 361, Seq. Id. No. 362, Seq. Id. No. 363, Seq. Id. No. 364, Seq. Id. No. 365, Seq. Id. No. 366, Seq. Id. No. 367, Seq. Id. No. 368, Seq. Id. No. 369, Seq. Id. No. 370, Seq. Id. No. 371, Seq. Id. No. 372, Seq. Id. No. 373, Seq. Id. No. 374, Seq. Id. No. 375, Seq. Id. No. 376, Seq. Id. No. 377, Seq. Id. No. 378, Seq. Id. No. 379, Seq. Id. No. 380, Seq. Id. No. 381, Seq. Id. No. 382, Seq. Id. No. 383, Seq. Id. No. 384, Seq. Id. No. 385, Seq. Id. No. 386, Seq. Id. No. 387, Seq. Id. No. 388, Seq. Id. No. 389, Seq. Id. No. 390, Seq. Id. No. 391, Seq. Id. No. 392, Seq. Id. No. 393, Seq. Id. No. 394, Seq. Id. No. 395, Seq. Id. No. 396, Seq. Id. No. 397, Seq. Id. No. 398, Seq. Id. No. 399, Seq. Id. No. 400, Seq. Id. No. 401, Seq. Id. No. 402, Seq. Id. No. 403, Seq. Id. No. 404, Seq. Id. No. 405, Seq. Id. No. 406, Seq. Id. No. 407, Seq. Id. No. 408, Seq. Id. No. 409, Seq. Id. No. 410, Seq. Id. No. 411, Seq. Id. No. 412, Seq. Id. No. 413, Seq. Id. No. 414, Seq. Id. No. 415, Seq. Id. No. 416, Seq. Id. No. 417, Seq. Id. No. 418, Seq. Id. No. 419, Seq. Id. No. 420, Seq. Id. No. 421, Seq. Id. No. 422, Seq. Id. No. 423, Seq. Id. No. 424, Seq. Id. No. 425, Seq. Id. No. 426, Seq. Id. No. 427, Seq. Id. No. 428, Seq. Id. No. 429, Seq. Id. No. 430, Seq. Id. No. 431, Seq. Id. No. 432, Seq. Id. No. 433, Seq. Id. No. 434, Seq. Id. No. 435, Seq. Id. No. 436, Seq. Id. No. 437, Seq. Id. No. 438, Seq. Id. No. 439, Seq. Id. No. 440, Seq. Id. No. 441, Seq. Id. No. 442, Seq. Id. No. 443, Seq. Id. No. 444, Seq. Id. No. 445, Seq. Id. No. 446, Seq. Id. No. 447, Seq. Id. No. 448, Seq. Id. No. 449, Seq. Id. No. 450, Seq. Id. No. 451, Seq. Id. No. 452, Seq. Id. No. 453, Seq. Id. No. 454, Seq. Id. No. 455, Seq. Id. No. 456, Seq. Id. No. 457, Seq. Id. No. 458, Seq. Id. No. 459, Seq. Id. No. 460, Seq. Id. No. 461, Seq. Id. No. 462, Seq. Id. No. 463, Seq. Id. No. 464, Seq. Id. No. 465, Seq. Id. No. 466, Seq. Id. No. 467, Seq. Id. No. 468, Seq. Id. No. 469, Seq. Id. No. 470, Seq. Id. No. 471, Seq. Id. No. 472, Seq. Id. No. 473, Seq. Id. No. 474, Seq. Id. No. 475, Seq. Id. No. 476, Seq. Id. No. 477, Seq. Id. No. 478, Seq. Id. No. 479, Seq. Id. No. 480, Seq. Id. No. 481, Seq. Id. No. 482, Seq. Id. No. 483, Seq. Id. No. 484, Seq. Id. No. 485, Seq. Id. No. 486, Seq. Id. No. 487, Seq. Id. No. 488, Seq. Id. No. 489, Seq. Id. No. 490, Seq. Id. No. 491, Seq. Id. No. 492, Seq. Id. No. 493, Seq. Id. No. 494, Seq. Id. No. 495, Seq. Id. No. 496, Seq. Id. No. 497, Seq. Id. No. 498, Seq. Id. No. 499, Seq. Id. No. 500, Seq. Id. No. 501, Seq. Id. No. 502, Seq. Id. No. 503, Seq. Id. No. 504, Seq. Id. No. 505, Seq. Id. No. 506, Seq. Id. No. 507, Seq. Id. No. 508, Seq. Id. No. 509, Seq. Id. No. 510, Seq. Id. No. 511, Seq. Id. No. 512, Seq. Id. No. 513, Seq. Id. No. 514, Seq. Id. No. 515, Seq. Id. No. 516, Seq. Id. No. 517, Seq. Id. No. 518, Seq. Id. No. 519, Seq. Id. No. 520, Seq. Id. No. 521, Seq. Id. No. 522, Seq. Id. No. 523, Seq. Id. No. 524, Seq. Id. No. 525, Seq. Id. No. 526, Seq. Id. No. 527, Seq. Id. No. 528, Seq. Id. No. 529, Seq. Id. No. 530, Seq. Id. No. 531, Seq. Id. No. 532, Seq. Id. No. 533, Seq. Id. No. 534, Seq. Id. No. 535, Seq. Id. No. 536, Seq. Id. No. 537, Seq. Id. No. 538, Seq. Id. No. 539, Seq. Id. No. 540, Seq. Id. No. 541, Seq. Id. No. 542, Seq. Id. No. 543, Seq. Id. No. 544, Seq. Id. No. 545, Seq. Id. No. 546, Seq. Id. No. 547, Seq. Id. No. 548, Seq. Id. No. 549, Seq. Id. No. 550, Seq. Id. No. 551, Seq. Id. No. 552, Seq. Id. No. 553, Seq. Id. No. 554, Seq. Id. No. 555, Seq. Id. No. 556, Seq. Id. No. 557, Seq. Id. No. 558, Seq. Id. No. 559, Seq. Id. No. 560, Seq. Id. No. 561, Seq. Id. No. 562, Seq. Id. No. 563, Seq. Id. No. 564, Seq. Id. No. 565, Seq. Id. No. 566, Seq. Id. No. 567, Seq. Id. No. 568, Seq. Id. No. 569, Seq. Id. No. 570, Seq. Id. No. 571, Seq. Id. No. 572, Seq. Id. No. 573, Seq. Id. No. 574, Seq. Id. No. 575, Seq. Id. No. 576, Seq. Id. No. 577, Seq. Id. No. 578, Seq. Id. No. 579, Seq. Id. No. 580, Seq. Id. No. 581, Seq. Id. No. 582, Seq. Id. No. 583，Seq. Id. No. 584，Seq. Id. No. 585，Seq. Id. No. 586，Seq. Id. No. 587，Seq. Id. No. 588，Seq. Id. No. 589，Seq. Id. No. 590，Seq. Id. No. 591，Seq. Id. No. 592，Seq. Id. No. 593，Seq. Id. No. 594，Seq. Id. No. 595，Seq. Id. No. 596，Seq. Id. No. 597，Seq. Id. No. 598，Seq. Id. No. 599，Seq. Id. No. 600，Seq. Id. No. 601，Seq. Id. No. 602，Seq. Id. No. 603，Seq. Id. No. 604，Seq. Id. No. 605，Seq. Id. No. 606，Seq. Id. No. 607，Seq. Id. No. 608, Seq. Id. No. 609, Seq. Id. No. 610, Seq. Id. No. 611, Seq. Id. No. 612, Seq. Id. No. 613, Seq. Id. No. 614, Seq. Id. No. 615, Seq. Id. No. 616, Seq. Id. No. 617, Seq. Id. No. 618,和 Seq. Id. No. 619。
20. 依照权利要求19任一项的方法，其中所述自身抗体识别选自下组的EGFR肽：Seq. Id. No. 3，Seq. Id. No. 8，Seq. Id. No. 9，Seq. Id. No. 10，Seq. Id. No. 11，Seq. Id. No. 12，Seq. Id. No. 13, Seq. Id. No. 14, Seq. Id. No. 64, Seq. Id. No. 102, Seq. Id. No. 309, Seq. Id. No. 370， Seq. Id. No. 373，Seq. Id. No. 519，Seq. Id. No. 520，Seq. Id. No. 521，Seq. Id. No. 525，Seq. Id. No. 556，Seq. Id. No. 558，Seq. Id. No. 561，Seq. Id. No. 566，Seq. Id. No. 600，Seq. Id. No. 602 和 Seq. Id. No. 603。
21. 依照权利要求17-20任一项的方法，其中该人受试者的血液样品中自身抗体的水 平比代表健康群体人受试者的所述自身抗体的水平高5倍。
22. 厄洛替尼或其药学可接受盐，特别是厄洛替尼盐酸盐，其用于治疗通过权利要求 17至21任一项的方法鉴定的NSCLC患者，包括对该患者施用厄洛替尼或其药学可接受盐， 特别是厄洛替尼盐酸盐。
23. 自身抗体用于预测NSCLC患者对厄洛替尼或其药学可接受盐，特别是厄洛替尼盐 酸盐，治疗的响应的用途，该抗体是通过权利要求17至21任一项的方法鉴定的。
24. -种用于在人受试者的血液样品中检测一种或多种选自识别人EGFR的自身抗体 组的自身抗体的水平的试剂盒，其中该人受试者的血液样品中所述选自识别人EGFR的自 身抗体组的自身抗体的水平与代表健康群体人受试者的所述自身抗体的水平相比升高指 示非小细胞肺癌。
25. 依照权利要求24的试剂盒，其中所述自身抗体识别选自下组的EGFR肽：Seq. Id. No. 3，Seq. Id. No. 8，Seq. Id. No. 9，Seq. Id. No. 10，Seq. Id. No. 11，Seq. Id. No. 12，Seq. Id. No. 13, Seq. Id. No. 14, Seq. Id. No. 64, Seq. Id. No. 102, Seq. Id. No. 309, Seq. Id. No. 370， Seq. Id. No. 373，Seq. Id. No. 519，Seq. Id. No. 520，Seq. Id. No. 521，Seq. Id. No. 525，Seq. Id. No. 556，Seq. Id. No. 558，Seq. Id. No. 561，Seq. Id. No. 566，Seq. Id. No. 600，Seq. Id. No. 602 和 Seq. Id. No. 603。
26. 依照权利要求25的试剂盒，其中所述自身抗体识别选自下组的EGFR肽：Seq. Id. No. 519, Seq. Id. No. 520, Seq. Id. No. 521，和 Seq. Id. No. 561。
27. -种在人受试者中诊断非小细胞肺癌的方法，其包括： a) 在该人受试者的血液样品中测量选自识别突变型人EGFR的自身抗体组的自身抗体 的水平， b) 比较所述自身抗体的水平与参照水平，并 C)当所述自身抗体的水平超出参照水平时提供非小细胞肺癌的诊断。
28. -种在人受试者中诊断非小细胞肺癌的方法，其包括： a) 在该人受试者的血液样品中测量选自识别突变型人EGFR的自身抗体组的自身抗体 的水平， b) 比较所述自身抗体的水平与参照水平，并 c) 当所述自身抗体的水平超出参照水平时推荐治疗。
29. 依照权利要求27或28的方法，其中所述自身抗体识别选自下组的突变型EGFR肽： Seq. Id. No. 554, Seq. Id. No. 555, Seq. Id. No. 556, Seq. Id. No. 557, Seq. Id. No. 558, Seq. Id. No. 559,和 Seq. Id. No. 560。
30. 依照权利要求28-29任一项的方法，其中所述自身抗体识别选自下组的突变型 EGFR 肽： Seq. Id. No. 1, Seq. Id. No. 2, Seq. Id. No. 4, Seq. Id. No. 5, Seq. Id. No. 6, Seq. Id. No. 7, Seq. Id. No. 15，Seq. Id. No. 16，Seq. Id. No. 17，Seq. Id. No. 18，Seq. Id. No. 19，Seq. Id. No. 20，Seq. Id. No. 21，Seq. Id. No. 22，Seq. Id. No. 23，Seq. Id. No. 24，Seq. Id. No. 25，Seq. Id. No. 26，Seq. Id. No. 27，Seq. Id. No. 28，Seq. Id. No. 29，Seq. Id. No. 30，Seq. Id. No. 31， Seq. Id. No. 32，Seq. Id. No. 33，Seq. Id. No. 34，Seq. Id. No. 35，Seq. Id. No. 36，Seq. Id. No. 37，Seq. Id. No. 38，Seq. Id. No. 39，Seq. Id. No. 40，Seq. Id. No. 41，Seq. Id. No. 42，Seq. Id. No. 43，Seq. Id. No. 44，Seq. Id. No. 45，Seq. Id. No. 46，Seq. Id. No. 47，Seq. Id. No. 48， Seq. Id. No. 49，Seq. Id. No. 50，Seq. Id. No. 51，Seq. Id. No. 52，Seq. Id. No. 53，Seq. Id. No. 54，Seq. Id. No. 55，Seq. Id. No. 56，Seq. Id. No. 57，Seq. Id. No. 58，Seq. Id. No. 59，Seq. Id. No. 60，Seq. Id. No. 61，Seq. Id. No. 62，Seq. Id. No. 63，Seq. Id. No. 65，Seq. Id. No. 66， Seq. Id. No. 67，Seq. Id. No. 68，Seq. Id. No. 69，Seq. Id. No. 70，Seq. Id. No. 71，Seq. Id. No. 72, Seq. Id. No. 73, Seq. Id. No. 74, Seq. Id. No. 75, Seq. Id. No. 76, Seq. Id. No. 77, Seq. Id. No. 78，Seq. Id. No. 79，Seq. Id. No. 80，Seq. Id. No. 81，Seq. Id. No. 82，Seq. Id. No. 83， Seq. Id. No. 84，Seq. Id. No. 85，Seq. Id. No. 86，Seq. Id. No. 87，Seq. Id. No. 88，Seq. Id. No. 89，Seq. Id. No. 90，Seq. Id. No. 91，Seq. Id. No. 92，Seq. Id. No. 93，Seq. Id. No. 94，Seq. Id. No. 95，Seq. Id. No. 96，Seq. Id. No. 97，Seq. Id. No. 98，Seq. Id. No. 99，Seq. Id. No. 100， Seq. Id. No. 101, Seq. Id. No. 103, Seq. Id. No. 104, Seq. Id. No. 105, Seq. Id. No. 106, Seq. Id. No. 107, Seq. Id. No. 108, Seq. Id. No. 109, Seq. Id. No. 110, Seq. Id. No. Ill, Seq. Id. No. 112, Seq. Id. No. 113, Seq. Id. No. 114, Seq. Id. No. 115, Seq. Id. No. 116, Seq. Id. No. 117, Seq. Id. No. 118, Seq. Id. No. 119, Seq. Id. No. 120, Seq. Id. No. 121, Seq. Id. No. 122, Seq. Id. No. 123, Seq. Id. No. 124, Seq. Id. No. 125, Seq. Id. No. 126, Seq. Id. No. 127, Seq. Id. No. 128, Seq. Id. No. 129, Seq. Id. No. 130, Seq. Id. No. 131, Seq. Id. No. 132, Seq. Id. No. 133, Seq. Id. No. 134, Seq. Id. No. 135, Seq. Id. No. 136, Seq. Id. No. 137, Seq. Id. No. 138, Seq. Id. No. 139, Seq. Id. No. 140, Seq. Id. No. 141, Seq. Id. No. 142, Seq. Id. No. 143, Seq. Id. No. 144, Seq. Id. No. 145, Seq. Id. No. 146, Seq. Id. No. 147, Seq. Id. No. 148, Seq. Id. No. 149, Seq. Id. No. 150, Seq. Id. No. 151, Seq. Id. No. 152, Seq. Id. No. 153, Seq. Id. No. 154, Seq. Id. No. 155, Seq. Id. No. 156, Seq. Id. No. 157, Seq. Id. No. 158, Seq. Id. No. 159, Seq. Id. No. 160, Seq. Id. No. 161，Seq. Id. No. 162, Seq. Id. No. 163, Seq. Id. No. 164, Seq. Id. No. 165, Seq. Id. No. 166, Seq. Id. No. 167, Seq. Id. No. 168, Seq. Id. No. 169, Seq. Id. No. 170, Seq. Id. No. 171, Seq. Id. No. 172, Seq. Id. No. 173, Seq. Id. No. 174, Seq. Id. No. 175, Seq. Id. No. 176, Seq. Id. No. 177, Seq. Id. No. 178, Seq. Id. No. 179, Seq. Id. No. 180, Seq. Id. No. 181, Seq. Id. No. 182, Seq. Id. No. 183, Seq. Id. No. 184, Seq. Id. No. 185, Seq. Id. No. 186, Seq. Id. No. 187, Seq. Id. No. 188, Seq. Id. No. 189, Seq. Id. No. 190, Seq. Id. No. 191, Seq. Id. No. 192, Seq. Id. No. 193, Seq. Id. No. 194, Seq. Id. No. 195, Seq. Id. No. 196, Seq. Id. No. 197, Seq. Id. No. 198, Seq. Id. No. 199, Seq. Id. No. 200, Seq. Id. No. 201, Seq. Id. No. 202, Seq. Id. No. 203, Seq. Id. No. 204, Seq. Id. No. 205, Seq. Id. No. 206, Seq. Id. No. 207, Seq. Id. No. 208, Seq. Id. No. 209, Seq. Id. No. 210, Seq. Id. No. 211, Seq. Id. No. 212, Seq. Id. No. 213, Seq. Id. No. 214, Seq. Id. No. 215, Seq. Id. No. 216, Seq. Id. No. 217, Seq. Id. No. 218, Seq. Id. No. 219, Seq. Id. No. 220, Seq. Id. No. 221, Seq. Id. No. 222, Seq. Id. No. 223, Seq. Id. No. 224, Seq. Id. No. 225, Seq. Id. No. 226, Seq. Id. No. 227, Seq. Id. No. 228, Seq. Id. No. 229, Seq. Id. No. 230, Seq. Id. No. 231, Seq. Id. No. 232, Seq. Id. No. 233, Seq. Id. No. 234, Seq. Id. No. 235, Seq. Id. No. 236, Seq. Id. No. 237, Seq. Id. No. 238, Seq. Id. No. 239, Seq. Id. No. 240, Seq. Id. No. 241, Seq. Id. No. 242, Seq. Id. No. 243, Seq. Id. No. 244, Seq. Id. No. 245, Seq. Id. No. 246, Seq. Id. No. 247, Seq. Id. No. 248, Seq. Id. No. 249, Seq. Id. No. 250, Seq. Id. No. 251, Seq. Id. No. 252, Seq. Id. No. 253, Seq. Id. No. 254, Seq. Id. No. 255, Seq. Id. No. 256, Seq. Id. No. 257, Seq. Id. No. 258, Seq. Id. No. 259, Seq. Id. No. 260, Seq. Id. No. 261, Seq. Id. No. 262, Seq. Id. No. 263, Seq. Id. No. 264, Seq. Id. No. 265, Seq. Id. No. 266, Seq. Id. No. 267, Seq. Id. No. 268, Seq. Id. No. 269, Seq. Id. No. 270, Seq. Id. No. 271, Seq. Id. No. 272, Seq. Id. No. 273, Seq. Id. No. 274, Seq. Id. No. 275, Seq. Id. No. 276, Seq. Id. No. 277, Seq. Id. No. 278, Seq. Id. No. 279, Seq. Id. No. 280, Seq. Id. No. 281, Seq. Id. No. 282, Seq. Id. No. 283, Seq. Id. No. 284, Seq. Id. No. 285, Seq. Id. No. 286, Seq. Id. No. 287, Seq. Id. No. 288, Seq. Id. No. 289, Seq. Id. No. 290, Seq. Id. No. 291, Seq. Id. No. 292, Seq. Id. No. 293, Seq. Id. No. 294, Seq. Id. No. 295, Seq. Id. No. 296, Seq. Id. No. 297, Seq. Id. No. 298, Seq. Id. No. 299, Seq. Id. No. 300, Seq. Id. No. 301, Seq. Id. No. 302, Seq. Id. No. 303, Seq. Id. No. 304, Seq. Id. No. 305, Seq. Id. No. 306, Seq. Id. No. 307, Seq. Id. No. 308, Seq. Id. No. 310, Seq. Id. No. 311, Seq. Id. No. 312, Seq. Id. No. 313, Seq. Id. No. 314, Seq. Id. No. 315, Seq. Id. No. 316, Seq. Id. No. 317, Seq. Id. No. 318, Seq. Id. No. 319, Seq. Id. No. 320, Seq. Id. No. 321, Seq. Id. No. 322, Seq. Id. No. 323, Seq. Id. No. 324, Seq. Id. No. 325, Seq. Id. No. 326, Seq. Id. No. 327, Seq. Id. No. 328, Seq. Id. No. 329, Seq. Id. No. 330, Seq. Id. No. 331, Seq. Id. No. 332, Seq. Id. No. 333, Seq. Id. No. 334, Seq. Id. No. 335, Seq. Id. No. 336, Seq. Id. No. 337, Seq. Id. No. 338, Seq. Id. No. 339, Seq. Id. No. 340, Seq. Id. No. 341, Seq. Id. No. 342, Seq. Id. No. 343, Seq. Id. No. 344, Seq. Id. No. 345, Seq. Id. No. 346, Seq. Id. No. 347, Seq. Id. No. 348, Seq. Id. No. 349, Seq. Id. No. 350, Seq. Id. No. 351, Seq. Id. No. 352, Seq. Id. No. 353, Seq. Id. No. 354, Seq. Id. No. 355, Seq. Id. No. 356, Seq. Id. No. 357, Seq. Id. No. 358, Seq. Id. No. 359, Seq. Id. No. 360, Seq. Id. No. 361, Seq. Id. No. 362, Seq. Id. No. 363, Seq. Id. No. 364, Seq. Id. No. 365, Seq. Id. No. 366, Seq. Id. No. 367, Seq. Id. No. 368, Seq. Id. No. 369, Seq. Id. No. 371, Seq. Id. No. 372, Seq. Id. No. 374, Seq. Id. No. 375, Seq. Id. No. 376, Seq. Id. No. 377, Seq. Id. No. 378, Seq. Id. No. 379, Seq. Id. No. 380, Seq. Id. No. 381, Seq. Id. No. 382, Seq. Id. No. 383, Seq. Id. No. 384, Seq. Id. No. 385, Seq. Id. No. 386, Seq. Id. No. 387, Seq. Id. No. 388, Seq. Id. No. 389, Seq. Id. No. 390, Seq. Id. No. 391, Seq. Id. No. 392, Seq. Id. No. 393, Seq. Id. No. 394, Seq. Id. No. 395, Seq. Id. No. 396, Seq. Id. No. 397, Seq. Id. No. 398, Seq. Id. No. 399, Seq. Id. No. 400, Seq. Id. No. 401, Seq. Id. No. 402, Seq. Id. No. 403, Seq. Id. No. 404, Seq. Id. No. 405, Seq. Id. No. 406, Seq. Id. No. 407, Seq. Id. No. 408, Seq. Id. No. 409, Seq. Id. No. 410, Seq. Id. No. 411, Seq. Id. No. 412, Seq. Id. No. 413, Seq. Id. No. 414, Seq. Id. No. 415, Seq. Id. No. 416, Seq. Id. No. 417, Seq. Id. No. 418, Seq. Id. No. 419, Seq. Id. No. 420, Seq. Id. No. 421, Seq. Id. No. 422, Seq. Id. No. 423, Seq. Id. No. 424, Seq. Id. No. 425, Seq. Id. No. 426, Seq. Id. No. 427, Seq. Id. No. 428, Seq. Id. No. 429, Seq. Id. No. 430, Seq. Id. No. 431, Seq. Id. No. 432, Seq. Id. No. 433, Seq. Id. No. 434, Seq. Id. No. 435, Seq. Id. No. 436, Seq. Id. No. 437, Seq. Id. No. 438, Seq. Id. No. 439, Seq. Id. No. 440, Seq. Id. No. 441, Seq. Id. No. 442, Seq. Id. No. 443, Seq. Id. No. 444, Seq. Id. No. 445, Seq. Id. No. 446, Seq. Id. No. 447, Seq. Id. No. 448, Seq. Id. No. 449, Seq. Id. No. 450, Seq. Id. No. 451, Seq. Id. No. 452, Seq. Id. No. 453, Seq. Id. No. 454, Seq. Id. No. 455, Seq. Id. No. 456, Seq. Id. No. 457, Seq. Id. No. 458, Seq. Id. No. 459, Seq. Id. No. 460, Seq. Id. No. 461, Seq. Id. No. 462, Seq. Id. No. 463, Seq. Id. No. 464, Seq. Id. No. 465, Seq. Id. No. 466, Seq. Id. No. 467, Seq. Id. No. 468, Seq. Id. No. 469, Seq. Id. No. 470, Seq. Id. No. 471, Seq. Id. No. 472, Seq. Id. No. 473, Seq. Id. No. 474, Seq. Id. No. 475, Seq. Id. No. 476, Seq. Id. No. 477, Seq. Id. No. 478, Seq. Id. No. 479, Seq. Id. No. 480, Seq. Id. No. 481, Seq. Id. No. 482, Seq. Id. No. 483, Seq. Id. No. 484, Seq. Id. No. 485, Seq. Id. No. 486, Seq. Id. No. 487, Seq. Id. No. 488, Seq. Id. No. 489, Seq. Id. No. 490, Seq. Id. No. 491, Seq. Id. No. 492, Seq. Id. No. 493, Seq. Id. No. 494, Seq. Id. No. 495, Seq. Id. No. 496, Seq. Id. No. 497, Seq. Id. No. 498, Seq. Id. No. 499, Seq. Id. No. 500, Seq. Id. No. 501, Seq. Id. No. 502, Seq. Id. No. 503, Seq. Id. No. 504, Seq. Id. No. 505, Seq. Id. No. 506, Seq. Id. No. 507, Seq. Id. No. 508, Seq. Id. No. 509, Seq. Id. No. 510, Seq. Id. No. 511, Seq. Id. No. 512, Seq. Id. No. 513, Seq. Id. No. 514, Seq. Id. No. 515, Seq. Id. No. 516, Seq. Id. No. 517, Seq. Id. No. 518, Seq. Id. No. 522, Seq. Id. No. 523, Seq. Id. No. 524, Seq. Id. No. 526, Seq. Id. No. 527, Seq. Id. No. 528, Seq. Id. No. 529, Seq. Id. No. 530, Seq. Id. No. 531, Seq. Id. No. 532, Seq. Id. No. 533, Seq. Id. No. 534, Seq. Id. No. 535, Seq. Id. No. 536, Seq. Id. No. 537, Seq. Id. No. 538, Seq. Id. No. 539, Seq. Id. No. 540, Seq. Id. No. 541, Seq. Id. No. 542, Seq. Id. No. 543, Seq. Id. No. 544, Seq. Id. No. 545, Seq. Id. No. 546, Seq. Id. No. 547, Seq. Id. No. 548, Seq. Id. No. 549, Seq. Id. No. 550, Seq. Id. No. 551, Seq. Id. No. 552, Seq. Id. No. 553, Seq. Id. No. 554, Seq. Id. No. 555, Seq. Id. No. 557, Seq. Id. No. 559, Seq. Id. No. 560, Seq. Id. No. 562，Seq. Id. No. 563，Seq. Id. No. 564，Seq. Id. No. 565，Seq. Id. No. 567，Seq. Id. No. 568, Seq. Id. No. 569, Seq. Id. No. 570, Seq. Id. No. 571, Seq. Id. No. 572, Seq. Id. No. 573, Seq. Id. No. 574, Seq. Id. No. 575, Seq. Id. No. 576, Seq. Id. No. 577, Seq. Id. No. 578, Seq. Id. No. 579, Seq. Id. No. 580, Seq. Id. No. 581, Seq. Id. No. 582, Seq. Id. No. 583，Seq. Id. No. 584，Seq. Id. No. 585，Seq. Id. No. 586，Seq. Id. No. 587，Seq. Id. No. 588，Seq. Id. No. 589，Seq. Id. No. 590，Seq. Id. No. 591，Seq. Id. No. 592，Seq. Id. No. 593，Seq. Id. No. 594，Seq. Id. No. 595，Seq. Id. No. 596，Seq. Id. No. 597，Seq. Id. No. 598，Seq. Id. No. 599，Seq. Id. No. 601，Seq. Id. No. 604，Seq. Id. No. 605，Seq. Id. No. 606，Seq. Id. No. 607，Seq. Id. No. 608，Seq. Id. No. 609，Seq. Id. No. 610，Seq. Id. No. 611, Seq. Id. No. 612, Seq. Id. No. 613, Seq. Id. No. 614, Seq. Id. No. 615, Seq. Id. No. 616, Seq. Id. No. 617, Seq. Id. No. 618 和 Seq. Id. No. 619。
31. 依照权利要求27-30任一项的方法，其中所述自身抗体识别选自下组的突变 型 EGFR 肽：Seq. Id. No. 1，Seq. Id. No. 2, Seq. Id. No. 4, Seq. Id. No. 5, Seq. Id. No. 6, Seq. Id. No. 7 和 Seq. Id. No. 15。
32. 依照权利要求27-30任一项的方法，其中所述自身抗体识别选自下组的突变型 EGFR 肽： Seq. Id. No. 16，Seq. Id. No. 17，Seq. Id. No. 18，Seq. Id. No. 19，Seq. Id. No. 20，Seq. Id. No. 21，Seq. Id. No. 22，Seq. Id. No. 23，Seq. Id. No. 24，Seq. Id. No. 25，Seq. Id. No. 26， Seq. Id. No. 27，Seq. Id. No. 28，Seq. Id. No. 29，Seq. Id. No. 30，Seq. Id. No. 31，Seq. Id. No. 32，Seq. Id. No. 33，Seq. Id. No. 34，Seq. Id. No. 35，Seq. Id. No. 36，Seq. Id. No. 37， Seq. Id. No. 38，Seq. Id. No. 39，Seq. Id. No. 40，Seq. Id. No. 41，Seq. Id. No. 42，Seq. Id. No. 43，Seq. Id. No. 44，Seq. Id. No. 45，Seq. Id. No. 46，Seq. Id. No. 47，Seq. Id. No. 48， Seq. Id. No. 49，Seq. Id. No. 50，Seq. Id. No. 51，Seq. Id. No. 52，Seq. Id. No. 53，Seq. Id. No. 54，Seq. Id. No. 55，Seq. Id. No. 56，Seq. Id. No. 57，Seq. Id. No. 58，Seq. Id. No. 59， Seq. Id. No. 60，Seq. Id. No. 61，Seq. Id. No. 62，Seq. Id. No. 63，Seq. Id. No. 65，Seq. Id. No. 66，Seq. Id. No. 67，Seq. Id. No. 68，Seq. Id. No. 69，Seq. Id. No. 70，Seq. Id. No. 71， Seq. Id. No. 72，Seq. Id. No. 73，Seq. Id. No. 74，Seq. Id. No. 75，Seq. Id. No. 76，Seq. Id. No. 77, Seq. Id. No. 78, Seq. Id. No. 79, Seq. Id. No. 80, Seq. Id. No. 81，Seq. Id. No. 82， Seq. Id. No. 83，Seq. Id. No. 84，Seq. Id. No. 85，Seq. Id. No. 86，Seq. Id. No. 87，Seq. Id. No. 88，Seq. Id. No. 89，Seq. Id. No. 90，Seq. Id. No. 91，Seq. Id. No. 92，Seq. Id. No. 93， Seq. Id. No. 94，Seq. Id. No. 95，Seq. Id. No. 96，Seq. Id. No. 97，Seq. Id. No. 98，Seq. Id. No. 99, Seq. Id. No. 100, Seq. Id. No. 101, Seq. Id. No. 103, Seq. Id. No. 104, Seq. Id. No. 105, Seq. Id. No. 106, Seq. Id. No. 107, Seq. Id. No. 108, Seq. Id. No. 109, Seq. Id. No. 110, Seq. Id. No. Ill, Seq. Id. No. 112, Seq. Id. No. 113, Seq. Id. No. 114, Seq. Id. No. 115, Seq. Id. No. 116, Seq. Id. No. 117, Seq. Id. No. 118, Seq. Id. No. 119, Seq. Id. No. 120, Seq. Id. No. 121, Seq. Id. No. 122, Seq. Id. No. 123, Seq. Id. No. 124, Seq. Id. No. 125, Seq. Id. No. 126, Seq. Id. No. 127, Seq. Id. No. 128, Seq. Id. No. 129, Seq. Id. No. 130, Seq. Id. No. 131, Seq. Id. No. 132, Seq. Id. No. 133, Seq. Id. No. 134, Seq. Id. No. 135, Seq. Id. No. 136, Seq. Id. No. 137, Seq. Id. No. 138, Seq. Id. No. 139, Seq. Id. No. 140, Seq. Id. No. 141, Seq. Id. No. 142, Seq. Id. No. 143, Seq. Id. No. 144, Seq. Id. No. 145, Seq. Id. No. 146, Seq. Id. No. 147, Seq. Id. No. 148, Seq. Id. No. 149, Seq. Id. No. 150, Seq. Id. No. 151, Seq. Id. No. 152, Seq. Id. No. 153, Seq. Id. No. 154, Seq. Id. No. 155, Seq. Id. No. 156, Seq. Id. No. 157, Seq. Id. No. 158, Seq. Id. No. 159, Seq. Id. No. 160, Seq. Id. No. 161, Seq. Id. No. 162, Seq. Id. No. 163, Seq. Id. No. 164, Seq. Id. No. 165, Seq. Id. No. 166, Seq. Id. No. 167, Seq. Id. No. 168, Seq. Id. No. 169, Seq. Id. No. 170, Seq. Id. No. 171, Seq. Id. No. 172, Seq. Id. No. 173, Seq. Id. No. 174, Seq. Id. No. 175, Seq. Id. No. 176, Seq. Id. No. 177, Seq. Id. No. 178, Seq. Id. No. 179, Seq. Id. No. 180, Seq. Id. No. 181, Seq. Id. No. 182, Seq. Id. No. 183, Seq. Id. No. 184, Seq. Id. No. 185, Seq. Id. No. 186, Seq. Id. No. 187, Seq. Id. No. 188, Seq. Id. No. 189, Seq. Id. No. 190, Seq. Id. No. 191, Seq. Id. No. 192, Seq. Id. No. 193, Seq. Id. No. 194, Seq. Id. No. 195, Seq. Id. No. 196, Seq. Id. No. 197, Seq. Id. No. 198, Seq. Id. No. 199, Seq. Id. No. 200, Seq. Id. No. 201, Seq. Id. No. 202, Seq. Id. No. 203, Seq. Id. No. 204, Seq. Id. No. 205, Seq. Id. No. 206, Seq. Id. No. 207, Seq. Id. No. 208, Seq. Id. No. 209, Seq. Id. No. 210, Seq. Id. No. 211, Seq. Id. No. 212, Seq. Id. No. 213, Seq. Id. No. 214, Seq. Id. No. 215, Seq. Id. No. 216, Seq. Id. No. 217, Seq. Id. No. 218, Seq. Id. No. 219, Seq. Id. No. 220, Seq. Id. No. 221, Seq. Id. No. 222, Seq. Id. No. 223, Seq. Id. No. 224, Seq. Id. No. 225, Seq. Id. No. 226, Seq. Id. No. 227, Seq. Id. No. 228, Seq. Id. No. 229, Seq. Id. No. 230, Seq. Id. No. 231, Seq. Id. No. 232, Seq. Id. No. 233, Seq. Id. No. 234, Seq. Id. No. 235, Seq. Id. No. 236, Seq. Id. No. 237, Seq. Id. No. 238, Seq. Id. No. 239, Seq. Id. No. 240, Seq. Id. No. 241, Seq. Id. No. 242, Seq. Id. No. 243, Seq. Id. No. 244, Seq. Id. No. 245, Seq. Id. No. 246, Seq. Id. No. 247, Seq. Id. No. 248, Seq. Id. No. 249, Seq. Id. No. 250, Seq. Id. No. 251, Seq. Id. No. 252, Seq. Id. No. 253, Seq. Id. No. 254, Seq. Id. No. 255, Seq. Id. No. 256, Seq. Id. No. 257, Seq. Id. No. 258, Seq. Id. No. 259, Seq. Id. No. 260, Seq. Id. No. 261, Seq. Id. No. 262, Seq. Id. No. 263, Seq. Id. No. 264, Seq. Id. No. 265, Seq. Id. No. 266, Seq. Id. No. 267, Seq. Id. No. 268, Seq. Id. No. 269, Seq. Id. No. 270, Seq. Id. No. 271, Seq. Id. No. 272, Seq. Id. No. 273, Seq. Id. No. 274, Seq. Id. No. 275, Seq. Id. No. 276, Seq. Id. No. 277, Seq. Id. No. 278, Seq. Id. No. 279, Seq. Id. No. 280, Seq. Id. No. 281, Seq. Id. No. 282, Seq. Id. No. 283, Seq. Id. No. 284, Seq. Id. No. 285, Seq. Id. No. 286, Seq. Id. No. 287, Seq. Id. No. 288, Seq. Id. No. 289, Seq. Id. No. 290, Seq. Id. No. 291, Seq. Id. No. 292, Seq. Id. No. 293, Seq. Id. No. 294, Seq. Id. No. 295, Seq. Id. No. 296, Seq. Id. No. 297, Seq. Id. No. 298, Seq. Id. No. 299, Seq. Id. No. 300, Seq. Id. No. 301, Seq. Id. No. 302, Seq. Id. No. 303, Seq. Id. No. 304, Seq. Id. No. 305, Seq. Id. No. 306, Seq. Id. No. 307, Seq. Id. No. 308, Seq. Id. No. 310, Seq. Id. No. 311, Seq. Id. No. 312, Seq. Id. No. 313, Seq. Id. No. 314, Seq. Id. No. 315, Seq. Id. No. 316, Seq. Id. No. 317, Seq. Id. No. 318, Seq. Id. No. 319, Seq. Id. No. 320, Seq. Id. No. 321, Seq. Id. No. 322, Seq. Id. No. 323, Seq. Id. No. 324, Seq. Id. No. 325, Seq. Id. No. 326, Seq. Id. No. 327, Seq. Id. No. 328, Seq. Id. No. 329, Seq. Id. No. 330, Seq. Id. No. 331, Seq. Id. No. 332, Seq. Id. No. 333, Seq. Id. No. 334, Seq. Id. No. 335, Seq. Id. No. 336, Seq. Id. No. 337, Seq. Id. No. 338, Seq. Id. No. 339, Seq. Id. No. 340, Seq. Id. No. 341, Seq. Id. No. 342, Seq. Id. No. 343, Seq. Id. No. 344, Seq. Id. No. 345, Seq. Id. No. 346, Seq. Id. No. 347, Seq. Id. No. 348, Seq. Id. No. 349, Seq. Id. No. 350, Seq. Id. No. 351, Seq. Id. No. 352, Seq. Id. No. 353, Seq. Id. No. 354, Seq. Id. No. 355, Seq. Id. No. 356, Seq. Id. No. 357, Seq. Id. No. 358, Seq. Id. No. 359, Seq. Id. No. 360, Seq. Id. No. 361, Seq. Id. No. 362, Seq. Id. No. 363, Seq. Id. No. 364, Seq. Id. No. 365, Seq. Id. No. 366, Seq. Id. No. 367, Seq. Id. No. 368, Seq. Id. No. 369, Seq. Id. No. 371, Seq. Id. No. 372, Seq. Id. No. 374, Seq. Id. No. 375, Seq. Id. No. 376, Seq. Id. No. 377, Seq. Id. No. 378, Seq. Id. No. 379, Seq. Id. No. 380, Seq. Id. No. 381, Seq. Id. No. 382, Seq. Id. No. 383, Seq. Id. No. 384, Seq. Id. No. 385, Seq. Id. No. 386, Seq. Id. No. 387, Seq. Id. No. 388, Seq. Id. No. 389, Seq. Id. No. 390, Seq. Id. No. 391, Seq. Id. No. 392, Seq. Id. No. 393, Seq. Id. No. 394, Seq. Id. No. 395, Seq. Id. No. 396, Seq. Id. No. 397, Seq. Id. No. 398, Seq. Id. No. 399, Seq. Id. No. 400, Seq. Id. No. 401, Seq. Id. No. 402, Seq. Id. No. 403, Seq. Id. No. 404, Seq. Id. No. 405, Seq. Id. No. 406, Seq. Id. No. 407, Seq. Id. No. 408, Seq. Id. No. 409, Seq. Id. No. 410, Seq. Id. No. 411, Seq. Id. No. 412, Seq. Id. No. 413, Seq. Id. No. 414, Seq. Id. No. 415, Seq. Id. No. 416, Seq. Id. No. 417, Seq. Id. No. 418, Seq. Id. No. 419, Seq. Id. No. 420, Seq. Id. No. 421, Seq. Id. No. 422, Seq. Id. No. 423, Seq. Id. No. 424, Seq. Id. No. 425, Seq. Id. No. 426, Seq. Id. No. 427, Seq. Id. No. 428, Seq. Id. No. 429, Seq. Id. No. 430, Seq. Id. No. 431, Seq. Id. No. 432, Seq. Id. No. 433, Seq. Id. No. 434, Seq. Id. No. 435, Seq. Id. No. 436, Seq. Id. No. 437, Seq. Id. No. 438, Seq. Id. No. 439, Seq. Id. No. 440, Seq. Id. No. 441, Seq. Id. No. 442, Seq. Id. No. 443, Seq. Id. No. 444, Seq. Id. No. 445, Seq. Id. No. 446, Seq. Id. No. 447, Seq. Id. No. 448, Seq. Id. No. 449, Seq. Id. No. 450, Seq. Id. No. 451, Seq. Id. No. 452, Seq. Id. No. 453, Seq. Id. No. 454, Seq. Id. No. 455, Seq. Id. No. 456, Seq. Id. No. 457, Seq. Id. No. 458, Seq. Id. No. 459, Seq. Id. No. 460, Seq. Id. No. 461, Seq. Id. No. 462, Seq. Id. No. 463, Seq. Id. No. 464, Seq. Id. No. 465, Seq. Id. No. 466, Seq. Id. No. 467, Seq. Id. No. 468, Seq. Id. No. 469, Seq. Id. No. 470, Seq. Id. No. 471, Seq. Id. No. 472, Seq. Id. No. 473, Seq. Id. No. 474, Seq. Id. No. 475, Seq. Id. No. 476, Seq. Id. No. 477, Seq. Id. No. 478, Seq. Id. No. 479, Seq. Id. No. 480, Seq. Id. No. 481, Seq. Id. No. 482, Seq. Id. No. 483, Seq. Id. No. 484, Seq. Id. No. 485, Seq. Id. No. 486, Seq. Id. No. 487, Seq. Id. No. 488, Seq. Id. No. 489, Seq. Id. No. 490, Seq. Id. No. 491, Seq. Id. No. 492, Seq. Id. No. 493, Seq. Id. No. 494, Seq. Id. No. 495, Seq. Id. No. 496, Seq. Id. No. 497, Seq. Id. No. 498, Seq. Id. No. 499, Seq. Id. No. 500, Seq. Id. No. 501, Seq. Id. No. 502, Seq. Id. No. 503, Seq. Id. No. 504, Seq. Id. No. 505, Seq. Id. No. 506, Seq. Id. No. 507, Seq. Id. No. 508, Seq. Id. No. 509, Seq. Id. No. 510, Seq. Id. No. 511, Seq. Id. No. 512, Seq. Id. No. 513, Seq. Id. No. 514, Seq. Id. No. 515, Seq. Id. No. 516 和 Seq. Id. No. 517。
33.依照权利要求27-30任一项的方法，其中所述自身抗体识别选自下组的突变型 EGFR 肽： Seq. Id. No. 518, Seq. Id. No. 522, Seq. Id. No. 523, Seq. Id. No. 524, Seq. Id. No. 526, Seq. Id. No. 527，Seq. Id. No. 528，Seq. Id. No. 529，Seq. Id. No. 530，Seq. Id. No. 531，Seq. Id. No. 532，Seq. Id. No. 533，Seq. Id. No. 534，Seq. Id. No. 535，Seq. Id. No. 536，Seq. Id. No. 537, Seq. Id. No. 538, Seq. Id. No. 539, Seq. Id. No. 540, Seq. Id. No. 541, Seq. Id. No. 542, Seq. Id. No. 543, Seq. Id. No. 544, Seq. Id. No. 545, Seq. Id. No. 546, Seq. Id. No. 547, Seq. Id. No. 548, Seq. Id. No. 549, Seq. Id. No. 550, Seq. Id. No. 551, Seq. Id. No. 552，Seq. Id. No. 553，Seq. Id. No. 554，Seq. Id. No. 555，Seq. Id. No. 557，Seq. Id. No. 559，Seq. Id. No. 560，Seq. Id. No. 562，Seq. Id. No. 563，Seq. Id. No. 564，Seq. Id. No. 565, Seq. Id. No. 567, Seq. Id. No. 568, Seq. Id. No. 569, Seq. Id. No. 570, Seq. Id. No. 571, Seq. Id. No. 572, Seq. Id. No. 573, Seq. Id. No. 574, Seq. Id. No. 575, Seq. Id. No. 576, Seq. Id. No. 577, Seq. Id. No. 578, Seq. Id. No. 579, Seq. Id. No. 580, Seq. Id. No. 581，Seq. Id. No. 582，Seq. Id. No. 583，Seq. Id. No. 584，Seq. Id. No. 585，Seq. Id. No. 586，Seq. Id. No. 587，Seq. Id. No. 588，Seq. Id. No. 589，Seq. Id. No. 590，Seq. Id. No. 591，Seq. Id. No. 592，Seq. Id. No. 593，Seq. Id. No. 594，Seq. Id. No. 595，Seq. Id. No. 596, Seq. Id. No. 597, Seq. Id. No. 598, Seq. Id. No. 599 和 Seq. Id. No. 601。
34. 依照权利要求27-30任一项的方法，其中所述自身抗体识别选自下组的突变 型 EGFR 肽：569.1(1.此.604,569.1(1.此.605,569.1(1.此.606,569.1(1.此.607,569. Id. No. 608, Seq. Id. No. 609, Seq. Id. No. 610, Seq. Id. No. 611, Seq. Id. No. 612, Seq. Id. No. 613, Seq. Id. No. 614, Seq. Id. No. 615, Seq. Id. No. 616, Seq. Id. No. 617, Seq. Id. No. 618 和 Seq. Id. No. 619。
35. 依照权利要求27-34任一项的方法，其中该人受试者的血液样品中自身抗体的水 平比代表健康群体人受试者的所述自身抗体的水平高5倍。
36. -种在人受试者中诊断非小细胞肺癌的方法，其包括： a) 在该人受试者的血液样品中测量选自识别人EGFR的自身抗体组的自身抗体的水 平， b) 比较所述自身抗体的水平与参照水平，并 c) 当所述自身抗体的水平超出参照水平时提供非小细胞肺癌的诊断。
37. -种在人受试者中诊断非小细胞肺癌的方法，其包括： a) 在该人受试者的血液样品中测量选自识别人EGFR的自身抗体组的自身抗体的水 平， b) 比较所述自身抗体的水平与参照水平，并 c) 当所述自身抗体的水平超出参照水平时推荐治疗。
38. 依照权利要求36或37的方法，其中所述自身抗体识别选自下组的EGFR肽：Seq. Id. No. 3，Seq. Id. No. 8，Seq. Id. No. 9，Seq. Id. No. 10，Seq. Id. No. 11，Seq. Id. No. 12，Seq. Id. No. 13, Seq. Id. No. 14, Seq. Id. No. 64, Seq. Id. No. 102, Seq. Id. No. 309, Seq. Id. No. 370， Seq. Id. No. 373，Seq. Id. No. 519，Seq. Id. No. 520，Seq. Id. No. 521，Seq. Id. No. 525，Seq. Id. No. 556，Seq. Id. No. 558，Seq. Id. No. 561，Seq. Id. No. 566，Seq. Id. No. 600，Seq. Id. No. 602 和 Seq. Id. No. 603。
39. 依照权利要求36-38任一项的方法，其中该人受试者的血液样品中自身抗体的水 平比代表健康群体人受试者的所述自身抗体的水平高5倍。
40. 依照权利要求28-35、37-39任一项的方法，其中所述治疗为厄洛替尼。
41. 说明书中描述的发明。
【文档编号】G01N33/574GK104334190SQ201380027063
【公开日】2015年2月4日 申请日期:2013年6月4日 优先权日:2012年6月7日
【发明者】P·伯恩特, B·克卢格哈默 申请人:霍夫曼-拉罗奇有限公司