专利名称：日本血吸虫表皮抗原基因、其编码蛋白及其应用的制作方法
技术领域：
本发明涉及生物基因领域，具体地涉及一类日本血吸虫表皮抗原基因、其编码蛋白，还涉及该日本血吸虫表皮抗原蛋白的应用。
背景技术：
血吸虫病由血吸虫的感染引起，在世界上有76个国家，受感染者约1.5亿。致病的血吸虫有3种，既日本血吸虫(Schistosoma japonicum)，曼氏血吸虫(S.mansoni)和埃及血吸虫(S.haematobium)。在我国流行的是日本血吸虫。血吸虫病的流行给人民身体健康和经济发展都造成巨大的损失，因此，防治血吸虫病、开发研制治疗血吸虫病和抗血吸虫的药物具有巨大的社会和经济效益。
研制新的治疗血吸虫病和抗血吸虫的药物，首先必须找到合适的药物作用靶点。血吸虫的表皮是宿主和血吸虫相互作用的界面。而组成血吸虫表皮的蛋白质暴露在这个界面上，对小分子的化学药物和大分子的抗体药物而言都是最理想的药物作用靶点。已经有研究表明治疗血吸虫的化学药物——吡喹酮的作用靶点就是存在于血吸虫的表皮。另外一些已知的血吸虫表皮抗原蛋白，例如GST28，paramyosin，已经用于疫苗研究和应用。我们通过大规模的蛋白质组学研究，发现了50个日本血吸虫表皮抗原蛋白质，这些蛋白质，都是可能的药物作用靶点和候选疫苗。
鉴于日本血吸虫表皮抗原蛋白的重要功能，研究和开发日本血吸虫表皮抗原蛋白具有重要意义。
发明内容
本发明要解决的技术问题之一是提供一类日本血吸虫表皮抗原基因。
本发明要解决的技术问题之二是提供一类日本血吸虫表皮抗原蛋白。
本发明要解决的技术问题之三是提供上述日本血吸虫表皮抗原蛋白的应用。
为了解决上述技术问题，本发明通过如下技术方案实现在本发明一个方面，提供了一类日本血吸虫表皮抗原基因，其编码具有日本血吸虫表皮抗原蛋白质活性的核苷酸序列，所述的核苷酸序列包括(a)SEQ ID No.1～SEQ ID No.34所示的序列；
(b)SEQ ID No.1～SEQ ID No.34所示的序列中每条序列的互补序列；(c)与SEQ ID No.1～SEQ ID No.34所示的序列中每条序列有至少70％同源性的序列，及(d)上述(a)～(c)中一条或数条的组合。
较佳地，所述的核苷酸序列包括具有SEQ ID No.1～SEQ ID No.34所示的序列。
在本发明的另一方面，提供了上述日本血吸虫表皮抗原蛋白，包括(a)具有SEQ ID No.35～SEQ ID No.68所示氨基酸序列的蛋白；(b)由(a)的蛋白发生一个或几个氨基酸置换、缺失或插入而形成的具有(a)蛋白相同活性的蛋白。
较佳地，其具有SEQ ID No.35～SEQ ID No.68所示的氨基酸序列。
在本发明的另一方面，提供了一类能与上述日本血吸虫表皮抗原蛋白特异性结合的抗体。在本发明中，可以使用一系列本领域已知的方法来制备针对日本血吸虫表皮抗原蛋白的抗体。例如，将提纯的日本血吸虫表皮抗原蛋白产物或它的抗原片段注射入动物体内以产生多克隆抗体。同样，表达日本血吸虫表皮抗原蛋白或它的抗原片段的细胞也可以用来对动物致免疫而产生抗体。本发明制备的抗体也可以是单克隆抗体，这些单克隆抗体可用杂交瘤技术制备。
在本发明的另一方面，提供了上述日本血吸虫表皮抗原蛋白作为免疫原，在制备血吸虫疫苗中的应用。
在本发明的另一方面，提供了上述日本血吸虫表皮抗原蛋白在制备防治血吸虫病的药物中的应用。应用本发明的日本血吸虫表皮抗原蛋白，可研究血吸虫治疗药物的作用靶点，从而在开发研制预防或治疗血吸虫病的药物的过程中发挥重要作用。
图1是本发明日本血吸虫表皮抗原蛋白的免疫荧光抗体原位杂交实验结果示意图。其中，T代表tegument(表皮)；G代表gut(肠道)；ST代表subtegument(下表皮)，绿色荧光部分是Immunophilin的信号。
具体实施例方式
下面结合附图和实施例对本发明作进一步详细的说明。应理解，这些实施例仅用于说明本发明而不用于限制本发明的范围。下列实施例中未注明具体条件的实验方法，通常按照常规条件，例如Sambrook等人，分子克隆实验室手册(New YorkColdSpring Harbor Laboratory Press，1989)中所述的条件，或按照制造厂商所建议的条件。
实施例1日本血吸虫表皮抗原蛋白的分离和鉴定实验1.日本血吸虫表皮的分离纯化血吸虫表皮的纯化按照Triton-x-100提取方法进行。表皮抗原蛋白从血吸虫雄虫、雌虫、雌雄混合成虫和童虫分离。虫体用Krebs’-Ringer’s，Tris-马来酸盐-缓冲盐液(KRTM)洗3次，再用KRTM/0.2％Triton X-100溶液悬浮。这个液体和虫体的混和物置于冰水浴中，在摇床上摇动10分钟。然后，装有液体和虫体的混和物的试管在涡旋混合器上混旋几次，然后再放回冰水浴中，静置使虫体下沉。用吸管吸出上部液体，放置到另一个新管中。这个试管在离心机上用2500×g的离心力，离心15分钟。得到的沉淀就是血吸虫的表皮。Krebs’-Ringer’s溶液的配方是0.15MNaCl，6.2mM KCl，1.2mM Na2HPO4，1.2mM MgSO4，10mM Glucose and 20mMTris-Hepes，pH 7.4。Tris maieate缓冲溶液的配方是0.2M Tris and 0.2MMaleicanhyclride，pH 7.4。
2.日本血吸虫表皮抗原蛋白的电泳分离日本血吸虫表皮用裂解剂裂解，蛋白溶液用SDS-聚丙烯酰胺凝胶电泳分离，条带切割后再做第二维的在线毛细液相色谱分离以及质谱鉴定。软件分析得到鉴定蛋白。分离好的血吸虫表皮，用SDS-聚丙烯酰胺凝胶电泳(SDS-PAGE)缓冲液悬浮，用超声波促进组织细胞裂解和蛋白溶解，超声波裂解方法为超声1秒，暂停3秒，总时间2分钟。并用100℃加热5分钟以使蛋白质变性。然后这些表皮抗原蛋白混合物用SDS-PAGE分离，分离方法按照普通的SDS凝胶电泳方法进行。胶上电泳泳道用刀切割下来，再用刀均等分割成8-12份。接下来做蛋白的胶内酶解。
3.日本血吸虫表皮抗原蛋白的胶内酶解过程胶内酶解按以下方法进行蛋白胶块用刀切碎，每个小块大小约为1立方毫米。加入100mM NH4HCO3，洗2次；转入到试管中；加入10mM二硫苏糖醇(dithiothreitol，DTT)/100mM NH4HCO3，56度放置反应1小时；冷却到室温，离心吸去DTT；加入等体积的55mM碘乙酰胺iodoacetamide/100mM NH4HCO3，室温/黑暗反应45分钟；吸去iodoacetamide，加入100mM NH4HCO3，洗2次；去掉NH4HCO3，加入乙腈缩胶；去掉乙腈，真空抽干；加入100mM NH4HCO3，胶膨胀，去掉NH4HCO3，加入乙腈缩胶；去掉乙腈，抽干；加入含Trypsin的消化缓冲液(50mM NH4HCO3/12.5ng/ul trypsin)冰上放置45min；去掉多余酶液，加入50mM NH4HCO3，以刚覆盖胶为准；37度，过夜酶切；酶切结束后，从试管中吸出上清液到新管子中，胶中加入60％ACN/0.5％HAC，在水浴中超声处理15min，再重复一次；吸出上清液到新管子中，在原管子中再加入60％ACN/0.5％HAC，37度40min，吸出上清液，与前面的上清液合并，真空抽气去ACN。
4.日本血吸虫表皮抗原蛋白酶解肽段的在线色谱分离和质谱鉴定酶解产生的肽混合物，用真空将溶液体积抽减到30μl。之后，每个组分的肽混合物上样到一个反相捕获柱(C18，5μm，300，300μmi.d×5mm，LC Packings)，在20μl/min的流速下脱盐。然后这个捕获柱和一个分析用的75μm×150mm C18柱(LC Packings)相连接，肽混合物就在200nl/min的流速下，从这个柱子上洗脱进入装有Protana NanoES的电喷雾离子源的QSTAR pulser i中。Agilent 1100毛细液相系统(Agilent Technologies)用来提供流动相A(0.5％乙酸/水)和流动相B(0.5％乙酸/乙腈)，线性梯度是60分钟内5％B到50％B，30分钟内50％B到90％B，然后是在90％B停留15分钟。在分析柱后通过一个不锈钢的两通(Valco Instrument)连接一个10-μm直径的PicoTip纳喷雾头(New Objective)，加上2500-3000伏的喷雾电压，以获得稳定的喷雾。MS/MS串联质谱谱图通过信息依赖的采集(IDA)和任务-循环增强来记录。在每个测定扫描中，选取3-6个信号最强、带2到3个电荷的离子，用滚动碰撞能量打碎，以获得串联质谱的信息。
5.日本血吸虫表皮抗原蛋白的软件搜索鉴定用牛胰酶的自裂解片段如([M+H]+)1153.5741，2163.0569和2274.1600作为内标，或者以预先数据库搜索得到的得分高的肽段的分子量，来校正所有的质谱谱图。质谱搜索用的数据库，包括从8420个从EST cluster预测得到的血吸虫蛋白质序列，以及可能的污染蛋白质序列如牛胰酶的序列、宿主蛋白污染如人蛋白质序列、小鼠蛋白质序列、大鼠蛋白质序列、兔子蛋白质序列和角蛋白序列等。搜索软件是MASCOT(http://www.matrixscience.com/，Matrix Science)。搜索参数为最大漏切点，3个；monoisotopic；肽电荷数，+1/+2/+3；修饰，半胱氨酸carbamoylmethylation和methionine氧化。搜索结果合并后，用下列标准进行过滤筛选1.对每一个串联质谱谱图的搜索结果列表，只提取排位在第一个的结果，其他的则丢弃；2.搜索得到的肽段中如果有KR，KK，RK，RR等序列，则这个肽段丢弃；3.搜索得到的肽段，长度在8个氨基酸以上的保留，小于8个氨基酸的丢弃；4.重复打到的肽段，只计算其中分值最高的，把这些肽段分值加起来，做为蛋白的得分；5.蛋白的得分超过60，就认为是可信的结果。低分的蛋白，手工检查后保留或者丢弃。匹配到宿主或者牛胰酶、角蛋白的肽段，则丢弃。
实施例2日本血吸虫表皮抗原蛋白的生物信息学分析和功能注释1.同源性和相似性分析日本血吸虫表皮抗原蛋白序列对GenBank(http://www.ncbi.nlm.nih.gov/)的非冗余数据库(Non-redundant，NR)做BLASTP比对；日本血吸虫表皮抗原蛋白序列对Swissprot数据库做BLASTP同源性比对。根据比对结果得到的与已知其他物种或已知的血吸虫蛋白的序列相似性，做出日本血吸虫表皮抗原蛋白的功能注释。
2.文献查检根据已经发表的文献报道，发现鉴定得到的34个表皮抗原蛋白在虫体上的位置，有一些的确在血吸虫表皮上。而其他则是新发现的表皮抗原蛋白。举例说明，比如以下表皮分离和质谱鉴定得到的蛋白，根据文献报道，就是已知的表皮抗原蛋白，而且有些蛋白已经有进行了疫苗和抗原研究Paramyosin，tropomyosin，myosin，dynein light chain 1，22.6kDa tegument membrane-associated antigen，tegumental antigen Sm20，nitric oxide synthase 1(NOS1)，leucineaminopeptidase，14-3-3 proteins，SnaK，cathepsin B-like cysteine proteaseprecursor，21.7kDa antigen，FK506-binding protein 50(immunophilin)。
实施例3日本血吸虫表皮抗原蛋白的免疫荧光抗体原位杂交实验为了进一步用实验的方法验证用蛋白质组学手段鉴定得到的血吸虫表皮抗原蛋白，选取了一个Immunophilin(SjFKBP50，GenBank登录号为AY815389)做免疫荧光抗体原位杂交做蛋白定位分析。
实验方法血吸虫成虫用冷冻包埋剂(OCT)完全包埋，切成5μm厚的切片。用抗Immunophilin的抗体覆盖切片，在37℃反应60分钟。该抗体是用重组表达的Immunophilin免疫小鼠制备的单克隆抗体。随后切片用PBS洗涤，再加入FITC-偶联的兔抗鼠免疫球蛋白的抗体(1∶40稀释，用含有0.5mg/ml的Evans蓝的PBS稀释)，置湿盒中37℃反应60分钟。染色结果用Leica DM-RB型荧光显微镜观察并拍照。
如图1所示，该实验结果证明了Immunophilin确实分布在血吸虫成虫的表皮上。这说明前面表皮分离和质谱鉴定的结果是可信的。
序列表<110>上海人类基因组研究中心<120>日本血吸虫表皮抗原基因、其编码蛋白及其应用<130>NP-10279<160>68<210>1<211>935<212>DNA<213>日本血吸虫(Schistosoma japonicum)<400>1gaaaaattac ttcatactgg tgtttatggg cttcatcaag ctgtactacg tagtatcggt60tcgttttatg taagctacga tcatatatca tttaattatc cacatgattc actttatcgt120gatattggtt ttcgtcgaat tcaattgaat aaaccaggta tttatgcaaa ttttgaaata180cgcttgttat cccgtgaagg gagtatctct tcacctcatt taatggctgt ggaactatct240gtatggcgtt ctcctagtct ttcagtgtta ttatctacat cattatataa tcacttatcg300aaagatatgt atgataacaa gcatattaat caggctataa atttcttcat taaactagag360aatgatgcat tcaatgattc tctctatgtt tgtccagccg cttgtcattc aacaccagtt420ccagtcaagt ttactagact catagtacca tttaccataa gtataccacc tactgctttg480atgtatcttg gaagcgaact caaagatctt ataaagtata gtcaaactct acattttgtt540gaagtatatg ccggcccagc acccaaacat gatataatta ctatgcacag acatggacac600gtgtttggtg ggttaccatg gcttttttgg atttctttgg tcttactgat cgctattttc660cacatgtttt tttgcaaaat catatacaat gaactgagaa ataagaacca gatgtctata720cctcgagatt cctggagact cccacgatat gcatctgact taatctcctc aacagctttt780gaaacaagag gcattagcaa cactcagata tcaagacgga ggataactga cctagacact840atcccgtgac tgaattctac cgagctttaa tgtttgattt ttagatcagt ttttttttat900aaataaagaa aacttttaga aaaaaaaaaa aaaag 935<210>2<211>596<212>DNA<213>日本血吸虫(Schistosoma japonicum)<400>2gtcactcaac attcgtctgt caccttgcca aagttgcatt tgccaaacta ccttcgttgt60taagtataga tgcacatgtg ttccttctga atcgtctact tagttttcct ctttaaatcc120tcatatattt caccgaacaa tccttccaac acggcgtgcc tatagaccgg acgcaaacta180ttaaaggctg ttcattacaa tgaagttatt ggacctaaag atcaatcgac aacacttaga240tcattcaaaa acaaaagata ttatatcgag agtttcttgt tttatcaaag aagccgagaa300agctgaagct gatgtgatgg aaggtgaagc tgacgatggt cgatacattg aattggattt360gttaatacct tcggttgcta gtgatgaacc actaaatctt ccagtcaatt tttcagactc420atcgactgag agcgacgatt cagaatcaga aggcgaattt cgtgaaataa ttgtaccaca480
tgcagaaaga cctggttcgt cagttcctcc tcgttgtaca tctcgcaaac tgattgaaga540actgaaatct atgtgtactg atgattgtat gatagaaaat aatacgtaaa ttgttt596<210>3<211>1340<212>DNA<213>日本血吸虫(Schistosoma japonicum)<400>3tcagccaacc cctatgatta ccactgataa taccgtttac aaatataaga agaatgttat60accttctgtg aaaacaatga acagcgtaca aaaaactcca agtcattcaa gtaataataa120aaatacagtc aaatatacta ctaaacgtac tattaagcaa caaccgaatc aacgtatttc180tatgacagca ccaccatcag ttaaaaatca ttctctagtc aacagtcgtt cagtatccgc240tgcgagttca acatctaatt tgaaatcaat tcatctatca aatgaatcaa atattgaacc300tttgcatagt attattccaa aaagtactaa tcccaaacaa atatctgcag agtctatctg360tgcagccctc gcgaaacaag agagatcatt gcgttataat ccttctcatt acttaactac420tcatgcgact acagccagtc atagtaaact catcaataac aattcaaata ttggaaatta480tgtcaaaagc aaatctggta ttcgatcatt aagtacatct agtcgaatac atcattctac540tgaacgttct attccaaaat cacaacagac gaaacaaagt tatccacttc atggatctaa600caattgtata cctgttgcac tatcctgtaa taactacact gctgacttaa acattaaaac660accagataca atcttacctg ctgaatcgtt cggatccggt caaatgataa caatagcgtc720gaaaaatgat gatattaacg tagatgttcc taaacctata cgtggcaaac gaaaatcctt780aataggacga aaaattagtt catctactgt tacagatacc tcagctgttg gaatacctgt840tgctgttaaa ccaatatcag ctgttaggcc gacactacat tcttcaaata acagtgaaaa900catcaaaact caaattctga tggtggataa aacaaaggta actactactg acacctccat960caaattgaat atcaccgata aggatgatgg aaatttatca ccaaaacaca ttgatgggta1020tacgaagtgt gattccacaa aaaataatta caattacaat aaatctattt tagataaaaa1080tgacgttgat atcgataagg ataagttgac atcgcctggt atgtggatta ttcgtgaaga1140tctcggcgtt attactgatg attttagtta aatcatattc taaaggttag aaaacaacct1200catacttctt ataattacta ttatcgagca atcataatac tgtcctccgg ttacatagta1260atcagttttc ctcgtttttt tttatttttg ctttgaatac tactttattg ttttttagta1320gtttcaaaat actggactgc1340<210>4<211>758<212>DNA<213>日本血吸虫(Schistosoma japonicum)<400>4tttcgatata atcattgtcc tactgtgatt atatatcatg ggaatgaaaa tgaagttgat60gattttcctg ataatgttaa ttatatcaat tgtttataca cagcctcaag tagaaatcga120tgtggaaatt ttaaatacta ccatgggaca acagaaagac aatagtgatt tgaggaacca180gggagatagg gtaagtgata aaagaagtga agatacttcg aggcatggaa agaatggtat240taggacagaa agcatgtttg gatcattctc atctagatat caatacaaaa aagcaaggga300ttttaatgcc agaggaaaat cccgatcacg aggtattgga agatatggtg taacgtcatc360agaatcaaca aattatgtaa ttgaaggaaa catcgataga tatggtcgac gaaaatcaac420
caattctaaa ttcaaaactc gtggaaaaga gaagaaatat tcaaggcgaa tcaatgacaa480tcggttcgat attaagggtg atttatatga acgacgtcaa catgtgggta ttggtgatta540tcaggcgaat ggtactcata ttagcgtcat gcttaaaact aaagaaaaga gtggcagttc600aaatggaacc atgattggca gagattcatc atcacattca aatagtactc aacgcagtga660acaaataatt tggggaaatc catgaaatag ttgtgatatt ttgacgggaa aattttacta720cattgttcag ataatcaaaa cttcttccca atgtcttc758<210>5<211>877<212>DNA<213>日本血吸虫(Schistosoma japonicum)<400>5gtaccctccc ataaagggga acaaaagctt gaggctccaa cggcccccgc ggcccgccct60tgaagtttgg ggatcccccg ggcggcagga attgggccca cgaggattat tttcactttc120atgatgattg acacatcaac taatatgaaa ttatccttta ttaatcgttc aacacaagtt180actgaagtac ttccaacatg tttagttgat gctcaatcat atacacgaca aataagtgaa240acattcgatg atataatgaa tcaacttgaa acatacaaaa atactccatt tattgactta300gatgatgttc aacttacaga taacaaatca ttagatcatc taattaaaga atcatttaca360tgctcaatat gtcgtggtct attagttcga gctcgtttat taccatgtgg tcatcatttt420tgtcgtgaat gcttatatta ctggttcaaa attcgtcatg catgtccatt atgtagaaca480tgtatacgta ctaatgtttc tgcattacat gttgataatt ttttggatga acttgttgat540cgtggatgta atgaagcatt aaaatgtcag cgtaaacaac gtaaagctga acaaaccagt600gcaatattag attgtaaata tgataggaga aactcagaaa atctactacg tactgttgta660gaaagttatg tacaaacaac ggcttatgtt gatagtactc aaagcaacgt atggactaat720tcttcataac gaacaaatta ttactttttc atttgaaagt acaaaccaat taaagtgaag780tgaataagtc ataatcattg acaatctcat caatcctctc ttttcttatt cctattctat840ggataattga ataaataaat actaatagta tagaggt 877<210>6<211>805<212>DNA<213>日本血吸虫(Schistosoma japonicum)<400>6ttttatagtt atcatcatta tcaatattca tttgcgtaaa tattcatatt ttgatattat60attaatttat aaaatggcaa ctcgtacaca agattttgga attgatgtag ccatatctag120aaagttgaat agccgatatg atccagatgc agaacgtcaa gttataggat ggatacaaca180attaactgga caaaatattc cacttggacg tgaaaatggt caaagaacat tggaaaatgg240tcggaatatt gagttgaact tatcaaatgc tgggggtatg aacgtacacc ggaatttggc300ctccaagaag cacaaaacaa taccgtagac ccaatgaatc cccaatacag gggaaatgct360cccatttaaa caaattggag aatattccaa ggggttcttg gtctatctga agcttatggg420tgaacacggg gaaggctttt tcacactggt gatttatttg aagcgagaaa tatggggcaa480gtattggcta ctttattggg attaggtaca gaatggcgac cgtatggagt tctaggggct540ggatgtggcc aaaaccacct attcacaact cgtcatgggc aggagaaaat tacgtgctgg600ggaaggatca attggcttct agcggaacaa aaaattaact tccccaaaag gtgggccttt660
gtgggccacg gctattcgcg ataattcatg ggaggaatga ctccccaagg ggctggtggt720attagttaca aaggggagga aaaatttggt cccaaaaggg agaagtttaa atcacggcta780ttggtgagat aaatgggggg ttagg 805<210>7<211>476<212>DNA<213>日本血吸虫(Schistosoma japonicum)<400>7gtgggggttt tattggcacc gctgcagctc ttgcattggg tttatctcag tcgctctaca60cagttgatgg aggtcatcga gcaattatat ttagtcgaat cgggggagtt caggatgaaa120tatatcccga aggcttgcat ttcaggtatt tcggtcgatt taaattcatc ttctttagaa180taccttggtt ccagtatcct attatatatg atattagatc gcgaccacga aagattacat240cacctaccgg tagtaaaggt cagttacttg tcttatctac attagcacgt gctattgtag300gattttcgta caataaatca gtaaatccac acgcgaagat tgctctctag ttcttagggg360atatcgtagt tttatttgat gatcgtcact gtgaacgtgg taggacttca gtataacttg420taaatgacaa aataatgttt aatgtttttt tatcagtata tcagccctcc aggccg476<210>8<211>859<212>DNA<213>日本血吸虫(Schistosoma japonicum)<400>8aaaagatcaa tttgttttat ttcttttgat aaaaactttg tttaataatt ttacaagcat60taactttatc attgaaattc ttgtgatatt attattagtt tttttcttct tcttcagatt120gtgtacaatt gtctattaga ttatgatgat gaataggtat tgaagtgtat tcaatttgct180gatgctgctg tattcttacg aattgtttca tgtactgcat ggattaatag ttctaattct240tcttctggtt gagtctcacc agatgatgaa atatcagtag aagtacctct tcatctgaac300cggttctctt catcttgatt atacaattta atgagattac ttttccatcc ttaaaatcca360cccaaaaatt gtggtcgcgt ctcaaaaaac ttcctggcta ccctttgaag ccagcctaac420atcttcattg ggctaattgt gcaatcatac tcgggcatat cacttttaat gggttcgaaa480aagttgcgcg cctcaggaaa acaacgaggc cgcccggctc caagggagac ctccgcaaaa540caaacctcga agtcgttcgg cgccaaaaag gcccaccgcg gaagccccaa ccccccaggc600acccctcaac attgccccgg cgccgaaacc agccccgcag ggcgcaagag agacaaacga660aaggacccca gacagagacg gccccggcgc ccaaaatcgc gcgccgcaga acacagaata720aagcacaccg ggccccccgc acggagacga agcgaccaaa cgaaccacac gcccgacgaa780accagagatc ccgccgcgcg acgcgcagaa caagcagccc caacccagcc ccgcgagaaa840aacataaaat aaccgccca 859<210>9<211>610<212>DNA<213>日本血吸虫(Schistosoma japonicum)<400>9
tgcagtttgt cgaccctcaa agttcatttg tttcaagaga ctatgtcaac gtttcccagg60gtgttcttcg atattacgat tggtggacaa ccgtccggaa aaattgtatt tgagcttttt120gacaacgttc cgaagactgc agagaacttc cgcgcattgt gcacagggga aaagggttat180ggatacaaaa actctacttt tcatcggatc attccaggct tcatgtgtca aggcggggat240tttactaatc ataacggcac tggtggcaaa agcatctatg gaaacaagtt tgaagacgag300agttttgaca ataagcacga taaaaaattt ctactttcta tggccaatgc tggacccaac360accaatggct cacaattttt catcactact gcagtctgtt cttggctaga cggcaaacat420gttgtctttg gtcgagttgt tgagggtgaa gatgtggtta caaagatgga aaaagttgga480tcacagagtg ggagaacaaa ccaagaagtc aaaatagtta attgcgggca atgctaattc540agatacttta aatgtatgtt gtggtttata aaattcattt tgcataatta aaaaaaaaaa600aaaaacaaaa 610<210>10<211>1125<212>DNA<213>日本血吸虫(Schistosoma japonicum)<400>10gctgttgaaa ttgctagact ctctggattt ccatttgtta aaattgttac ttcacataaa60atgattggat ttactgaaat ggctaaatgt gctgcaatga aaaagatttt tgatgatggt120gctaaatcac ctttaagtgt cgtcattgtt gataatattg aaggcttaat tgaatacaat180ccagttggac caagattttc taattttatt gttcaagcga ttcgtgattt agtttctcaa240ccattgaaag ggggtcgtca tatgttagta ctggctacaa cgagttgtcg tgaggcattg300acagatcaga atcttactca agcattttca tggcatgttc atgtcggcat gttatcaaaa360cctgaacata taattgctgc tctggaagaa gatggacgat ttacacctaa agaacaacaa420tatattgaaa aatctttatc tggtcgacgt ttatgtattg gaataaagca tttacttgac480ttgattgatc tcgtttcgaa gaatgatgca aatcatcgtg ttgctgcatt tcttgctaaa540ttagaagaag tcggtattgt tgatgaataa aaaacataag cacacaatga aaattattca600tttgttcatg atggttgtgt tcgtggaaat aaatttgatc aagagcttca attccatgat660atttatttaa agtttataat cattccatgc aaactagttt acactttcct caatactttt720gatggctgtt attatcatta ttttattttg tttgtattcg ttttcatcga ataacgttct780cttctctttt tgaattattt cactatatga agtgtgcaca cacagtctat cttactcatt840tatttgtgac tatcgctaat cttcattaca taaaatgtta tgtaatcatt gcctattaca900catagtgttc ttattattct ttctacttgt gtatacattc ataaattcct acctttattt960acttattact tatgcattac aaagtcttat ttacttcaca tgaaacattc attaacttct1020atattcgtca taatgaatct gttcagaata gacatattgt gataataaag aatttgttta1080tttattaaat aaaatctata cacattcaaa aaaagaaaaa aaaaa1125<210>11<211>1942<212>DNA<213>日本血吸虫(Schistosoma japonicum)<400>11ctggcgcgcg ttcacatgtt cgcacattgg tgtgtttcat aatcatgttc atttcagttg60tacagttttg aatcttggac tgtgacgaat gtgttcgttt tcaatgttac attacttcgt120
cggtaacgac ataattcatt aatgcatctt gaaagtcttt gtccgtacta acttagcttt180ccaatgagaa ctatagatgt ttcagttaga tccacagctg aaccactact ttcaccaaac240gtcgctggat ttgtatgtgg tggcatttcg ggaacagttt ctcgtactgt tacagcacct300atggatcgtt taaaggtcct cagacaaatg gataccccag agatagcagg caaggacatg360attgcaagtt tacgtatatt gattaaagaa ggcggcattt tatctttatg gagaggtaat420ggggtgaata ttttaaaaaa ctgtcctgaa agtgctattc gtttcggtct tcatggatgg480ctgaagtcag taatttttcc taatgtgaaa ggaccactat ctcccaaaga tcgtctgttg540gttgctagtt tggccggtgc cacatcatta accataactt atccagttga aatattaaaa600acacgaatgg ctctacgaag gtcacacgaa actaattcta ttttgtctgt gatccgaata660ctatatactg agggcagtct acgtggattt tatcgaggct ataaaatcag tatgatgagc720tatgtgccgt attctggaaa tggcggcagc ttatgccttt atgagatgct taaacaacgc780tatgtagaat atcattattc ccttcaacct aacacagatc gtacaattaa ggttcatatg840ccagctatgg taactgttcc attgatttta atatcgtgtt gtattccaat gataattact900tatcctgcca atttacttcg cacacgatat caagcatcgt catctccgta tgccgctcct960atcataaaat ctatgtgttc tattgtacaa aaaaatggat tcaaaggact ttatcgtgga1020ttaagtgcta gtttatcaaa aactgtgcca tctgtctgtg ttacttatct tgtttttgag1080tccacttctg aattattagg tgttcctgga cttggctgta agtgaagcaa caaaataatg1140atagctgatg ttcaatttct taaaaaaatg tatttatcga acactttgtc tattcaacaa1200cttatatctt gaatttttcg aaaataggct atttgcattt gcattttttt tctgaccttt1260cgatcagcta aacgtttgaa agtaatcgtt tcttttattc attagtaatt gtgacatgct1320gaaacgtctt tcaaaaaaca taaacctatt ttcatcaacg atttttgtac ttcagctgta1380actttttcaa ttagagtaca actaaaatga tgtatatcgc atatactatc actcttttcc1440tttttccttc attatttttc tctctttgtc ctcttatttg ttttcgattc ttcgtcaatt1500atttctcggt ggttaccact tctatttttg tgttgctatt tagtactaag atttcctctc1560gaattttctg gtcaatcagt cagtcatgta catgatcttc tggacagttt gtccattctt1620ttcctgtatt agtcacttac ggcaagccaa cccatagcct ttgactgcgt aattttttta1680accttattag ttttttgagt tttaattcat ttatataatc cttagaaaat aatccgtgaa1740aagaattgta tttagttatt ttctcagagt tttagcaaga ataaacaaat aacaatatgt1800atatcgtttg agacaaatat tcagactaaa taaaatcaaa acttaaacat ttaaaattgg1860ggattgcttc tatacaaagt ccatattagt tatttgaatt attttcaatt tataatatag1920tgaaacgaag tactctcagt tc 1942<210>12<211>627<212>DNA<213>日本血吸虫(Schistosoma japonicum)<400>12acaaccaata atcacaattc tggttaatct ccgtaaaacg tgctcagcta taaataacct60gctttgatct ctctgttaac tacactactt gcctatactt agtcaaaatg tctaaagtca120aggttggaat tataggcggt tctggatttg atgacccaaa tctcttcaag caagtaggga180ttagaaaagt tttgtcggtg atgttgcatg tgttgtactt cccaggtagc caatgtttga240gtaaatttgt ttctttcata attattcaca taatctacta ttgtaatcta ctggctactc300attttctgta cttggacttt tatttattga tcaagtaatg atgttattca tttctaggat360atattcagcc aattttactt gctcatatat tcactcttca tagtcattca tataaaatta420
ttgatgacgg ttttactact gtctgaatac ctgactaatc tccttttagt tcaacaaaaa480tgtacaacaa gatgtaaaga aatatgccga aaaaggaaat atcaacattg tgagggagtg540caagtatatg ggaaaatgta ataacaaaga catggaaagg gacatttaat cctgccaagt600gaagttaatt atagagccaa catttgg627<210>13<211>751<212>DNA<213>日本血吸虫(Schistosoma japonicum)<400>13tattctacta tttgaaaatg ccacgattcc caccttatct aactgaagct caagaggatg60acctgcgcca aatcgctcaa gctatatgtg cgccgggaaa gggaattctt gcagctgacg120aaagtacagc cacgatgggc aaacggcttc agcaaattgg cgtcgaaaat aacgaagaga180accgtcgctt atacagacaa ctgttgttta gcgctgacca taaactcgcc cagaatatat240caggggtaat attattcgag gaaacattac accaaaaatc tgatgacggc aaaactcttc300ctacatttgc taagcggaac gacacattca ttaccccggg gaaataaaaa aaggttgtac360caaaaagggg gagggttttt cccccccccc ttttgggggg gtttgtccca atattaaaat420aaggaggaga aaaaacccac ccccctcgtg ccgggggggg ggccccgccc ttcttaaaaa480ttatttctgt taactacttc tacccaatcc gtccacccct tatttaagta taacaatgcc540cggttgtcca acatttctct ccttatccct taaccccaat gtccatactc attcttatca600cgccggccct ttgccagatt tgcccatctt catgatacct ttcccaccca ccatatacac660gccgagctac gtaccactcc tctcgtagcc ccgcttctat taatccccgc tttccgcata720atcattgaca cagctctcgt ttagctgcaa t 751<210>14<211>654<212>DNA<213>日本血吸虫(Schistosoma japonicum)<400>14gatcagcaga tcaacgtgca attattgcac aaaaatataa atcattattc ggaaaagatt60tgattacaaa attgaaatct gaaattagtg gacattttta cgatacaatg gaagcactat120gttgtagtcc tgcagaattt gatgctcgtg aactacacag agctatgaaa ggtgctggta180cagatgaatc tgtattgatt gaaatattat gtacacgtac aaatcatcaa atacgccaaa240taaaagaagc gtatggaagg tgtaagtata atgaaatcgc gataatatct tgtgaattag300tatcatttgt gtatactgat tatacataat taccacctat actgtttctt cctatcaaac360tttaaaagtt gtacaggttt ttttctttac gttaagtgtt tactgcacat tatcatacct420ttcatgtcat atatctctaa atattttagt ttgactacgt acacaaagat tttacatgat480ataccttaat aatcaaacta caaacattat ttactggatt ccaataactg tttgagccaa540ctacttaata tcaaatgaaa atcaatgtac ccattggtgt caacattatt aacgaatcaa600aacaatgtaa aattttgaaa cattacaaaa aaaaaaagcc ttgattattt tgtg 654<210>15<211>1330<212>DNA
<213>日本血吸虫(Schistosoma japonicum)<400>15ggggttataa aaacagctaa atatcttgct tctagtatgc gcgatggcta tatgattgaa60tttaccccac gttcagacat caccgttgac tactgggact atacaagtta tgacgtttca120gaaaagtctc atttttcaaa acaacaggaa aaaattgagt tcaattatca ttataataaa180agtatcatga ttagagacat cggatttgtg atcaaagttc atgggcacat cttaaaatca240gcttacatat cgcaacatgg tgttgtgtat atggctaatg aatttaaaac cggcagttca300atgagatcct ttggaatagt aggtgcgatt tcaaggaaag gagattttgc tacagctact360aaaaatgata cgttctatat tatatggtat gatttgaaaa tctgcaaatt tgaccctgaa420atattagtcg atttacaaat gcgcttttat aaaaacggaa ttgttcaaat tgaattggta480catgtatatg ggagacctac gaattgtaat ttaacaatgg aaattctcga tggattttgt540caaatgaata gagatggaag tgtcactatg gtggagaaaa aagtttggaa attagccagt600tatccaagtt ctagaatatt ccttggcaat aggtttgaat ttaccccacg ttcaaatttg660tcctgggttt attgatgtag cgtatacaac atcaactgag tcaatgactg atcaaatgta720actttttgat ttcagtactg tctttttttt gtcttgttta actggtactt agtagtgtta780tcatgtgctt gtaatcttgt ttgttcttta tccgatctac cagttatagt ttttctacta840gttttagttt tttgtagtat ttatagtttt tctacttttt gtattttggt tgatcataac900gagaaacaaa cgttaaattt gattttgttc ttatttattt attgaatgtt aagtgaagta960gcaaatgatt gtcttcataa tttaaatttg ttttaatgta aatgtaaaag atgaaataca1020aagtgtgatt agagaatgtt ttgtttctgt tatgccggac ttctatagaa aagtcacgaa1080ctctcattac tgtgttttag aacactgttg taattattct aacttaggtt aatgtgaaca1140ttttgtctcg atgattgtca cattcctctt actctactct cccgattagt atactttccc1200tttagtatat tgtctcttac aatgaactac agttaatttg ttgtgtacaa gttatcccta1260tttactgcca ctatttcttc agttctgtct tgatgttaat ttcctaattt atcttctgtg1320tggtctggtt 1330<210>16<211>712<212>DNA<213>日本血吸虫(Schistosoma japonicum)<400>16agatttccta ccagatattc aagaagactt gtcatcatcc tctgcttcac aaactgccac60aatgaaaggt attagtaatt taggaacagt gactagtggt gaaacaacaa aattggatct120tccatctgcc ggtgcaacaa gatcaatgtt gcgtttgaaa aagctgataa atgttaagaa180tactccattt caaacacgtg atccattttc attaccagtc aaacgtttgt ggtgttattt240agttcgacaa cctgatctgg atgatacgtt tttgcgtcat atatttcgta aaccgtatct300tgtacaatca gttattacac caaatattaa attctcactg tcttcattat caactacttc360tggaccacca gaacttatca ctacacctgg cttagcaaag tctacatcat catctaattt420gaaaaatatc tcttctacta cactgacgga cattcaaaag tcatctggaa aacaaacacg480aacacaccaa caaactcaat ctacaaatca aactgctgga tcatctcttc ttctcaacga540cgctgttcgt ctcattcata aagaacatga cccacttata gctatgtgta ttaatcagga600ctgccgggct tcgagcaacc acgaccctat aagggtcgaa agctaggacc aatatgtctt660gtgggaaacg cttaacgtcc atttcaccag attggcgtct aatagtctac at712
<210>17<211>1308<212>DNA<213>日本血吸虫(Schistosoma japonicum)<400>17gccagtaatt ttcattcgcc caatatgcct gcccaaaacg caatactatt accctttatg60gatgcggcat gtagagtacc ccaaggtttt ccctggagac cctcctgtcc aatgtttttt120gccgccgttc tggatgaaaa tgatgtatcc cggtccgaag aatcctcctg atcatgttgt180ttttaaagtc catccacaaa tgacgaaacg tgatgtatgg cagtatttgg agaaaatata240caatgtccct gtagttgatg tccgagtgaa aatggtttat cacgatttag aaccggttga300tatatcagtg gaagagcgaa ctcagcgttt gaatgattct acaaactttc cacctaaacc360tgaacgttat gaaaaggttg cctatgttcg tttggctcct ggtgaagtct ttgttttccc420cgatctattc aaagacaaac gtccatcaga ccccttagat gttctagacg atgattttgt480taatgaacct aaaaatcgtc aaggaaaagc cacttcccac agttccagaa agttagaaac540taacaaactt caacccttaa aaggtggtat taagtggttt ggattctaat aattgtacat600attgaaacga ttgttcatat tgattgttat attaggtgta aatgaagtga ctttagggct660tgaaaattct gtgtttgatt aattaatgat acttgattaa aatgtgaatt gtttaactat720gatttaaatg gaaggtttat gaagtaagtg aatgatgatg tgatgtttga gattattgtt780agtggtggta gtcaggtaaa caaatgatgg aatgttagaa tacactaaaa gatcatgttt840attgtttatt atgagttgct catgaagtga ttgatatgcc tgaactacgt gaaaaataag900ggaatggaag tgtacatgtt ttctgtgatc atagttatac agatacactt gtatacaaac960agtagaatat tgtacgttat atgcttcatg aattgtttat tatgtagaat ttgactgtaa1020acggttgtga atatagaaga aaatgtttac tttaatctaa tgaaacagtt tcatagtatc1080aatctaaatg tgttcgtgat gtttgaagtc tacatttcaa ataacaccat gatacagata1140agttgatgaa ctgtatttgt cagtcatcat gttgttgtat tttatgggtt tatattgtga1200taactaaact ggctgatatg acttcttact aaaacgaaca tttactataa cttagaagta1260agaaataacc attcgctatt agttgcctag tcaatgttga tcatattc 1308<210>18<211>1478<212>DNA<213>日本血吸虫(Schistosoma japonicum)<400>18ttctagccca actggtgttt cagttgaatt gccaatggat gatggaaatg aggtgattgt60tttgcgagga aaacctgaag atctgggacg tgctctttca atggtttatg aacgagctca120aagcactatc aaagaggaaa tagaagcacc caatcgcttc catagacttt taattggaag180aggaggctct aaattaacag aattactgga aggatataaa cgagttcagg tgaattttgg240cgagaataca gacagaatct ctgttgaagg tcctagtgaa gaagttgaag ttattgttga300gcgtctaaag agtcgcttgg cagagttaca agccactgtt gcaatgacga cggttaaagt360agatccaaag tactacagac acataatcgg taagcaagga gctaccatcg gtcgtctccg420tgactacaaa gtccgcgtca gacttccaga tccagaccga ggcgattctt ttgcatgtga480tgaaatagtg atagagggtg atccagtcgg agttgaaaag gcaaaacttg aaatccaaca540actagtggag agattggaaa atgagaaatg taaagatgtg attattgatc cacatatcca600aaatttgcta cgctcaaact cgattggaac tactactccc tatattcgca ctatttacga660
catgttccct caggtacgca ttatttggcc tgagagtgat acaaatgatt ccatgtttga720agaaaaccca actaaaagta ttgtccaact ccgtggtgat cgacagcaag ttgatgcagc780tagtgaaaag ttacaaaaaa ttattaaatt agtcaaagag gagaattata gacaagagat840acatatcttt aaagagatac gatccagttt tcttggacgt gaatatccaa gggtgcgtaa900aattcttgaa gatacacaaa cacgtctaca gcatcctaat cccggttcga attctgacat960tttcactgtt attggacgag aggaaaatgt ctgtaaagcg attgtacaat tagaagaatt1020gcaaaaaaaa cttgctagtg ttaaagaagt aatcgtttct atcccatcag cattaacaac1080aaaatttgct ggagaccaag caccaagtct aagatcaatt tgtgaacagt gtgaaggtgt1140acatataaga tttgcccaca ataaaaattc caagggacaa tcacataaat taagtcatat1200tgatatagtt gtactgggac cagagaaaga agttaatcaa gcatgcagct tattaagtga1260actaaacgca aaagtatcgg aattatgcgc ccgaggatgt tgttcatgct gatccaaaat1320ttaggggagt tgtggagaag gtagacgtgg atggaaaata attggtgttt gggggggcgg1380gaaggcgaga agggaggtag cgctagatag gggttggagt ggagggggtg tgggggaggg1440gggtgggggg gaagaagggt gcgggaatga agtggggg1478<210>19<211>756<212>DNA<213>日本血吸虫(Schistosoma japonicum)<400>19ttgctagatt gtggagattt taaaccagcg cgtggctttt gatcgatggc ttctatatct60cttttgaacc ctaaagcaga gtttgcgaag gcgcaacatg catttggtat taatttagca120gctgcgaagg gactttatga tgttttaaag actaaccttg gccccaaagg aaccatgaaa180atgcttgttt caggtgcagg tgacattaag ataaccaaag acggcaatgt tctgcttaat240gaaatgcaga ttcagcatcc tactgcaagt ctgattgctc gtgtagccac tgctcaggat300gacatgacag gtgatggcac aacatcaaat gtgcttctaa ttgctgagtt gttaaaacag360gctgacgttc acacttctga aggacttcat ccacgactta ttacggaagg atttgacatc420gctcggaata aatgtttgga aatactatct aaatgtcgaa ttgattgtcc ttcggaaatg480ccagacagag ccatactcat ttcggtagcg gctacatcac ttaatacaaa agttcattct540gacttggcta accttctgac tgagcatgtt gtggatgcag tactgtcaat tcgacgacca600aacgaacctt tggatttgca tcgtgttgag ctgatgcaaa tgcaacataa aaccgatatg660gatacgacac tcgtgaaagg attggtatta gatcatggtg gtagacatcc agatatgccg720aaaacgtgta caaatgcatt tatcttaaac gtgcac 756<210>20<211>663<212>DNA<213>日本血吸虫(Schistosoma japonicum)<400>20ttgtatttac ctcaaaccac ctaaaaatac caaccaatag aaatcggaac attttcggcg60cggtccttga cgttttttgt cttgagttat tttgatatta atgctgttgt cgtcttcaat120ttgttagaca tacagccaac tactgtattg cagaaaattc acagtacaga gtaaaaaaca180tcagaagctt actgttatat aaatacagat ggtttgcgaa aagtgcgaga agaaacttgg240aaaagtaata actcccgatc catggaaagc gggtgcgcgg aatacagtca gtagtggtgg300
gaggaaactg aatgagaata aactgttaac cagttcgaaa aaccgattta gtccttattc360taatacgttt ccgagatgca gaatatgtgc acaaactgtt catcaaccag gctcctatta420ttgtcagcaa tgtgcattca agaaaggtat ttgttctatg tgcggtgtgc gtttaatcaa480tacgacacaa tataaccagt cagctgccta attattttat ctttctgaca tttaaaattt540tattttttag ttggaatgtc tgtaaaaggt gtacataata tcgtgcactt ccagattcgt600tggttattag ttatattctg attaaaagtt taatactaat ttagtataca ctctaaataa660act 663<210>21<211>547<212>DNA<213>日本血吸虫(Schistosoma japonicum)<400>21tcgaaacata tcttttagaa aaggctcgtg ttattcgcca ggcatcagat gaacgcagtt60ttcatatatt ttaccaactc ttggcttcag ctactccagc aatgcaacaa cagttgttat120tgaatcacgc tagttcttat cgttttctat caaatggtat gattgaactc cctggagttg180atgaacaaca atttttccga gaaacaactg atgccatgga tataatggga attaataatg240aagaccaaaa cgccatattc cgtgtaatat ctgctgtact tcacctagga aactttgaat300ttcgccagga acgaaattct gatcaagcaa ctttacccga ccattcagtt gccgaaaagg360tatctcatct tttgggcatt cctttaaatg aaatggtcaa agcatttttg aaaccccgca420tcaaggttgg aaaagatctg gtatcaaaag ctcaaactaa aacccaagtg gaatttgctg480ttgaagcaat ctccaaatct atctatgaaa gactatttat ttggctagtt gcacgaataa540ataaaac 547<210>22<211>667<212>DNA<213>日本血吸虫(Schistosoma japonicum)<400>22ttgacatctc agaaagcgtt actacttttc attacattca tattattaca gtatgatact60gagtgtagaa aatcgttatt aaatgtagaa gatcctgaaa catggaggaa gttagcagac120gagaaattta ataaatttga aaaatcactg tattattcat taaccaaacg accgaaaaat180gtcatcatat tcattggtga tggaatgagt ttgaatacag tcactggagc aagatatttg240aaagcagaga atatggatct tttgggtggt gatgtacaat tagtgtggga tgattggcct300gtagcatcac tagttcgcac atttaattca gatagattga ccacagattc aggttctgca360gcaactgcat tcttgtcagg tgcaaaaggg cctgatggta ctgttggtat tactggaaca420gtaaaatgtt gtaaatgtac tgagttaaga gatttagaga gagcaaaatc ttcacttaaa480tatgcttcca atgctggatt atccactgga atagttacaa ctactagagt tactcatgct540acaccggcag cagcatatgc gaatttatta catcgagatt gggagtcgaa tgcagaaatc600agcgatagtg gattcaattg ttcagatgct gctgcacaac taatcgctaa tgcatctgac660atcaatg 667<210>23<211>636
<212>DNA<213>日本血吸虫(Schistosoma japonicum)<400>23atgtgaaaat acctatcata ggcatagatt tactaagcca ccacaacttg cttgtggacg60ctagcaaaaa gaggttagtc gacagcagca caaagctgta cgtacgtgga accactgcta120caggttgtag cctatgtcct cttcgcttta aacatttaat taacccttat tttcagccaa180tactcgacaa gtatccggac ctgaataaaa cgatatcgaa gttatcatgt gtaaccagca240atgttaaaca tcacatcacg actacaggtc aacctgtatt ctccagagca cgccgattag300cacccgaaaa atcaaggatt gctaagaacc atttcgacca tatgattgac ctgggaataa360tccgcccatc cagcagccca tggtcgtctc cattacatat ggtcccgaag aaagacagca420atgattggcg tcctaccggc gattaccgac gtcttaatgc acaaactacg ccaaatcgtt480accctttacc tcacatccac gacctaacgg ccacattaaa gggaatgaca gtattctcaa540aaattgatct agtaaaagcc gaaaacggct attgtaaccc cgttcggtct ttacgaattc600ctccgaatgc ccttcggctt gaggaatgca gcacaa 636<210>24<211>719<212>DNA<213>日本血吸虫(Schistosoma japonicum)<400>24acatatttat tctctcttca gcttttttat ttgccttagg tcctatggct tctccacaga60tgccaatcaa gttccaagaa catttacagc ttgttagtgt tggaattcaa cctagtagca120taggttttag taccctgact atggagtcgg ataaatttat ttgcgtcaga gaaaagactg180gtgatacatc acaagttgta attattgata tgaatgatcc aatgaatcct acacgtcgtc240cgatttctgc tgatactatt ataatgaacc ccgtaagtaa ggtaatggca ttaaaagctg300gaaagttgtt gcaaatattc aatatcgaat taaaaagcaa aatgaagact tatacaatgc360cagaggatgt tgtattctgc aagtggatat ccgtgaatac tattgcgctc gtaactggca420ctagcgttta ccactgggct atggatggtg attcgaaccc agtcaaaatg tttgatcgtc480accaatctct tgggaatacg caaatcataa gttatcgttg tgatcttagt cagaagtggc540tattgcttat tggaattatt gcacaagaca accgtgtcgt tggatatatg caactctata600gtgtggagcg aaaagttagc caggcaatag agggacatgc agctgctttt gctcgttatg660tacctgaagg aaacactaac cctactactt tgtctgtttt gcgtcgcgca acgcacagg 719<210>25<211>693<212>DNA<213>日本血吸虫(Schistosoma japonicum)<400>25cataatggga cagcgtatgt atttaccact tgcagaaaaa atgttaatca gggaagccga60gggagggaaa atgtccagac attcagagct tcaggttcta attgacttac tcacccgcct120tcataaatat gaagaggctt ataagcttct taatcgaaaa gatataacag atcatataga180tgcggaggac tacatttgtg attactcata tacgaaactc agtttgttgg ctcatttagg240catgtgggat gatctttacg aactagcaaa gtcccaaact gaaatcaatc ctgaggactg300gacgccatgg aagaaattga ttgaagcgtc tttagaagat atacagaggg tggaaaaagt360
tatgtcattg atcaatactg ttataacaaa tcatcctagt gctcgtggtc cgcatttagc420acgcttagac ttgtatgcaa acactctgaa tctgggaatc catttgagtt gtaattacga480tccattgaaa ttaatgctgg actactttaa tatattctgt cataagccta tatgttctat540ggatttagct taccttgtgc gtatggtcct accgaattcg gatgatcaaa tcaagtatgt600tagtctcatt atggaaattg ccgagactgg tttggattta caaacaatga ggataaggca660gtttatcgtc ttttatgtgc tcagcaaata gct 693<210>26<211>683<212>DNA<213>日本血吸虫(Schistosoma japonicum)<400>26ccgaaacgtg atggatcaca tgcctttgtc agagttaagc taccacctca cacgggatcc60attatcatat cggacagatt ttgaagcaca gttggaaaac tttaacacac ttaaacagtc120attttcttct gccccttctc aatacataag tcgacttgag gatcttttgt cattcatatc180ccaggctgta aggttttatc cacagcatgt tgtggagttt gccactggtg ttattcaaac240actattgtca cggtcatttg gcatgcaccc ggaaatgcgc atggcatttt taagagcgtt300tatgcggatt cgaacgagaa acttaatttc tgcaacacaa gcggtcgatg ttgcatttaa360attgcataga tgtcgagata aacaagttag gaaaacattg cgtcacttcc tcgtaagtga420tatcaaaaga atgaataaaa gtcagaaaca gaccaaagct aatgcaatta ttctgtcatt480cctttcgaag atgatcaaag ataacagttc gactgtagca cgggaagctg ttgtaacctt540actttgtcta ttcaagaaga atgtttggaa cgacgcacgt actgcgaatg ttattgcaga600ttcatgttta atgagtaaca agaaggtcta tgttcctgct attcagtttt tcttgggaaa660gtcaaaggct ctaaatgaaa tgg683<210>27<211>477<212>DNA<213>日本血吸虫(Schistosoma japonicum)<400>27tgccattttt aacatatgaa gaaatagaac aagcagaaca tgatggtgtt cagtttgcta60aagaagctct ttctaataaa accaaaaaac caaaatctga ttcaccagaa tttgtcctaa120atgaaatgtc acatggaaaa gcaacatatc tccaggcctt tgaatcatta tgccactatt180caaatatacc atgtgtaaca gtgaaaggct tagctaaagg tgttgattat gtagtcggta240tgcgtttgaa tgatatcaat gaacaatcgc aacaaacatc atccgtcatg catcgattgc300aacatgcatg gaatgctgct tatttagata ataaatgggc attattcgat ccaatgtggg360cggcacaacg attagctgtc tctgcaaata cacgtttatc acagttagta caaactggac420aaatggatta tgaaacagat atgttttact ttaatggcga tccagcgaaa tttattt 477<210>28<211>638<212>DNA<213>日本血吸虫(Schistosoma japonicum)<400>28
cgacatcaac aattagcaca gaatgaagtt gctcaacaat atctattcga tgcaagtgaa60gccgaagcct ggatgggaga acaagaactg tacttaatgg gtgatgaacg tgcaaaggat120gaacaaggcg ctaacaatgc aattaaaaag catgaacaat tgcaaaaaac tgtggagaat180tacgcctcag aaatccgtgg tttaggtgaa cgatgccagg ctctgttgga agccaaccat240ccagaatctg aagctgttgc tgctaaacaa gcacgtattg acaaaatgta tgcaggttta300cgtgaccttt gtggtgaacg acgatcaaga cttgatgaaa ttctcaaatt gtatcatctt360ttacgagata ttttagatct tgaagcttgg attgccgagc gtatcttgat agcaagcagt420catgaagttg gcacagatta cgagcattgc tgtttgcttc gagaacgttt cgccgaattt480tctcgcgaaa cgaatgaact tgggaaatat cgcattagcg cagcaaatga attgtgcaac540gctcttattg gtcaaggtca tagcgaggct gctgagattg ccagttggaa agatcgaatc600aatgaagctt gggctgactt actagaactc attgacac638<210>29<211>910<212>DNA<213>日本血吸虫(Schistosoma japonicum)<400>29tatgctcgct caagcctatg agtgtgtctt tttgaaagct gagaaagatg agaagaagcc60tgcaatttta gcaagaatat catcaacatt gactaatctt tatgaggact gtttggttaa120gtgttcgggt ggggctaaaa atgttgttcc gaaggagtgg cctggtgtat taagcatgaa180aaaaggtcta tatgaagcat tgactcaata ttatcaatct aaagattgtt gtgaaagtaa240acaatatggg gaacaagtgg ctcgcttatc tttggcctat gaattaatca aaggtgctgc300tcgaaatagt tgttttccgc gaaaatattt ggttgaacaa ctgaaacatg aagaagcagt360tgcaacaaaa gacaacgatc acatatatca cgacaacgta ccgactaaat tgagtcttcc420tgctgttcaa agtgttgatg tcataaaaaa aactccacta gcattccctt tatcatcatc480tcagaataaa gattattttg aagaacttct tcctatcgct gttacagaag cagttaacac540ttccaaaggt gttcgtgcat ctttgatcga tggcgaaatt tgtaagctac aagaagcatc600tgctaaagtg aatgatgcgt tagcttcgta taacttaccg gcagcgttac aagccaaaga660atcaaattca attttagatt ctattttatc caaagctgtt tctattcgtc atgaaggcgg720ggcagagaaa ttatatcaac agctaatgtc aatacctgaa tgtgtccaac gtaataaaga780aatcattgaa gctgagagat ctgcgttaga cgatgaaaga gaagtcagat gatagcttac840gaacgcaata tggtgaacgt tggtcacgga aaccatcaaa agacttaaac caattatggg900cgttctgata 910<210>30<211>676<212>DNA<213>日本血吸虫(Schistosoma japonicum)<400>30tgttgataaa gttggaagta ttccagaaga agtgggacgt gatgctaagg ctgttgcagc60tcttcaacgc aagcaccata cgtttgaatg tgaattagca cgtcttggat cacgtgtgga120agggatcgtt gaaacagcta acaccttact accatcttat gccgcagata aagaacgtct180tatttgtgac cgtcgagacg aggtaattca tgcatggcga caactacaat gtgctacgga240acagcgtaaa atacacttgc atgatgcatc tgatattcat cgtttcttct ctatggtgcg300
tgaactccgc ttatggatgg aaactattcg catggaaatg actactaaag aaaaaccaag360ggatgtatct ggagtggagt tgctaatgaa cagtcatcgt agcttaatgg ctgaaataga420tgcccgagag gagaatttca gtatttgttt aagtttaggt cgtactctac ttaatcgtcg480acatcctcga gaagaagaag ttaggaaaaa atgcattcag ctggtgactg aaaaaatcct540tctaaatgac caatgggcag aacgatggga aacactaggt ctccttatgg aagtatatca600gtttgcccgt gacgctgaaa tagcagatac ttggcttgta tctcaagaag cttatcttaa660taatactgat ttgggc676<210>31<211>1093<212>DNA<213>日本血吸虫(Schistosoma japonicum)<400>31gcggattatg ataggtctgg tatatttatt gttagaatat tgtccttttt tgatggagtc60agccaaagaa ataggaccgt caagcttaca acacttctta tctcagagta ttgccagctg120ggacccagaa catcgggtta atgaagttcc agctagtaat gattgtcttc tccgaataac180taaagatttg cgttccattt gcaatgaccc agctcctgga atatgtgtag tacctgataa240tgaagatatg acaaaaatat acgcattaat tacaggtccc tttgacacac cttatgaagg300tggttttttc ttgttcctta tacgatgccc tcccgaatat ccactcacgc caccgaaggt360gaagttgatg actactggga atggtactgt gagatttaat ccaaattttt attcgaatgg420aaaggtttgc ctaagtgttc tcggtacttg gcaaggtccc gaatggacac cagcacaaag480cttatcctca gttttaatat caattcaatc tttgatgaat gataaaccgt attttaatga540acctggattc aacattgaac gtaatccggg cgatgcccag caatacaatg caataattac600acatgaaact atccgatgtg cagtatgtga tgttttagaa ggccgtacaa tatttcctcc660ggatttatat gcagtagtgg aatctagttt tgaagactat tatgaatact ataccagtgt720ttgtgaacgg aatatgcatt tatcaggtca acctatggta gatccatttg aagatggacg780aggaatcttt gattatgcta atctattaaa acgtttaaag gctttaaaca acaaagtgaa840acaaaagcat tctgggataa atgaagctaa tccgatgagt ataagagaaa gcaaataata900aaaatgagct ccgaactaac tgtaattggt tgataactta gaaggacatt ttgaatacat960tattgtatat ctgagttttt tcgaaatcta ggtgacttac gttccaattt ttcgtaaata1020tctttttcac agtcctccaa aagtcaactt taacttaatt aactctcatt tcactttaaa1080aaaaaaaaaa aaa 1093<210>32<211>603<212>DNA<213>日本血吸虫(Schistosoma japonicum)<400>32tgagcggaag agtacaaaca aatattatcc ccccgatttc gaccccaatc gtcacaggaa60tttaaattca taccatggaa cacatgccct tggtcaacgt gggaaaaagg cagatcaagg120tgtcatcact attaggtttg aaatgccttt caactgctgg tgtctcactt gcaaaaaccc180tatcgggatg ggtgtcagat acaatgcaga aaagaaaaaa gttggaatgt atcactctac240tcctatctac caattttcta tgccttgtca tctctgtgct ggtacaattg taatgcagac300agacccgaaa aactttcaat acgttatttt ggaaggtgct agacgcaagg tacagaaatg360
ggattctgag gaaaatgaac aagtacttat tccaaatcac tttgaaaaga agaagctttc420tacagatgcc atgtaccact tggaacatga tgtaactgac aagaagaaag cacttgaggt480cttgccagcg atacaagaaa ttcaaacaga tcgtttggca cacgaagatg acttcgcatt540gaatcaaatc gctcgtaaaa gatttcgtgt atgaattggt ttgggtttct ttagtatccc600ttt 603<210>33<211>922<212>DNA<213>日本血吸虫(Schistosoma japonicum)<400>33gttcttagca agcaatagtt gaagttgatt gtttgagtac acgaattagt tacgcgggac60cactgtttca ttttctgtta acatgtcatc ttttcggatg gcaggacgtg ttgtaggaca120gctgtccaga ataaatttat gtgcatttaa acagtttgga gaccgatgga tatccactac180atcggcttta aaagccgcgg tcccagagtt ctctagtatc ttagaggaac gtattttagg240acaaacctta caaactaact tggaagagac tggacgtgtt ttaagcatcg gtgatgggat300cgcacgtgtt tatggtttga aaaacataca agcggaagag atggtagagt tttcgtcggg360attgaagggt atggctctaa acttggagcg tgataacgtt ggtgtagtcg ttttcggtaa420tgacaagcta attcgtgaag gcgatattgt caaacgagca ggatccattg ttgatgtgcc480tgttggagaa gagttacttg gtcgggtagt tgatgcttta ggtactccaa tcgatggggc540aggtgctatc aatacaaaga caagacagcg tgttggagtc aaagctccag gtattatacc600acgtatttct gttcgtgaac ctatgcaaac tggaattaag gctgtcgata gtcttgttcc660catcggtcgt ggtcagcgag aactaatcat tgggagatcg tcagacaggt aaaacagcta720ttgcagtaga tactattatc aaccaaaagc gtttcaacga ggcagctgat gaaaagaaaa780agctatactg tatctatgtt gccatcggcc aaaaaacgtt caacagtttg cacagctggt840caaacgtctg acagacacgg atgctatgaa atacacaatt attgtatctg caacagcatc900tgtgcagcac ctctttatac aa 922<210>34<211>1219<212>DNA<213>日本血吸虫(Schistosoma japonicum)<400>34cggttttact cttgtgattt gttgcatgct ttattcaatt ttcaatgaaa tttcgtatta60aaacaaatga tcctggatgt attgagtatt tctcaaatat tgttcaaatg gtcgctggat120tagctaagaa tgtcatcttg catttgaata aaggtcagtt atcatttgtt attaaagaac180gagtagtatt tggcgggatt acagcatggt gtgatttaga gcatgcagta ttattttctg240aacgtatatg tgaaggaata tcaccggatc aagatgaaat attcctagaa ttaattatgg300atcatttatt gcattgtatt cgttctaata cactatctac atctaatcat aattctttat360acagttcatc ttctacatcg aattcatgta caacaatgaa taaatcacgt attggttcta420attctttaac tggtaatttt gatccatcaa tttgtcgatt acttggctta aaaattaaat480tagttcgacg taaaacacct tgtttagcaa ttgaattgga acagtcaagt gttacaggtc540gttcacgttc tgtttggcat tttattcctg ttcaagttgt acctccacgt ttgtgggatg600aatttataga accaccagat ccagatttcg atgtgagtat ttttttccct cctgtgaaaa660
cattacgccc tttcgttgat cgtatgaaaa agtttgcaaa gtttgttatt atcagcgcta720atggttcagg tgaattaaat tttagtatca acgttgaatc attagcctgt gttaaattaa780catttcgtgg tttaaaagca cgtagttgga tgttacaaga atcactattg tcagaaatca840acgattcaat ggatgaagtt cgtagtactg tctgggattc aaatgatgta gatttagata900gatcaaaaca aataaacgac gatccaaata agaaattagt atcagttagt ttagatttac960gacgcattag tcagttgtta tcaagtccaa agattgtacc atcgtgtttt gtttgtaata1020ttattcatga aaaattagct caattcttaa tgttatttga caatcacaaa ttgaaattca1080atatcccagc atctaattta taataataaa attatcttgt ttgtttgtga ataactttgt1140aattaatatt atactaaaaa agattctaat atacaaatat actacttatt tttctaaaaa1200aaaaaaaaaa aaaaaaaaa 1219<210>35<211>282<212>PRT<213>日本血吸虫(Schistosoma japonicum)<400>35EKLLHTGVYGLHQAVLRSIGSFYVSYDHISFNYPHDSLYRDIGFRRIQLNKPGIYANFEI 60RLLSREGSISSPHLMAVELSVWRSPSLSVLLSTSLYNHLSKDMYDNKHINQAINFFIKLE 120NDAFNDSLYVCPAACHSTPVPVKFTRLIVPFTISIPPTALMYLGSELKDLIKYSQTLHFV 180EVYAGPAPKHDIITMHRHGHVFGGLPWLFWISLVLLIAIFHMFFCKIIYNELRNKNQMSI 240PRDSWRLPRYASDLISSTAFETRGISNTQISRRRITDLDTIP 282<210>36<211>129<212>PRT<213>日本血吸虫(Schistosoma japonicum)<400>36MKLLDLKINRQHLDHSKTKDIISRVSCFIKEAEKAEADVMEGEADDGRYIELDLLIPSVA 60SDEPLNLPVNFSDSSTESDDSESEGEFREIIVPHAERPGSSVPPRCTSRKLIEELKSMCT 120DDCMIENNT129<210>37<211>385<212>PRT<213>日本血吸虫(Schistosoma japonicum)<400>37MITTDNTVYKYKKNVIPSVKTMNSVQKTPSHSSNNKNTVKYTTKRTIKQQPNQRISMTAP 60PSVKNHSLVNSRSVSAASSTSNLKSIHLSNESNIEPLHSIIPKSTNPKQISAESICAALA 120KQERSLRYNPSHYLTTHATTASHSKLINNNSNIGNYVKSKSGIRSLSTSSRIHHSTERSI 180PKSQQTKQSYPLHGSNNCIPVALSCNNYTADLNIKTPDTILPAESFGSGQMITIASKNDD 240INVDVPKPIRGKRKSLIGRKISSSTVTDTSAVGIPVAVKPISAVRPTLHSSNNSENIKTQ 300ILMVDKTKVTTTDTSIKLNITDKDDGNLSPKHIDGYTKCDSTKNNYNYNKSILDKNDVDI 360DKDKLTSPGMWIIREDLGVITDDFS385
<210>38<211>215<212>PRT<213>日本血吸虫(Schistosoma japonicum)<400>38MGMKMKLMIFLIMLIISIVYTQPQVEIDVEILNTTMGQQKDNSDLRNQGDRVSDKRSEDT60SRHGKNGIRTESMFGSFSSRYQYKKARDFNARGKSRSRGIGRYGVTSSESTNYVIEGNID120RYGRRKSTNSKFKTRGKEKKYSRRINDNRFDIKGDLYERRQHVGIGDYQANGTHISVMLK180TKEKSGSSNGTMIGRDSSSHSNSTQRSEQIIWGNP 215<210>39<211>202<212>PRT<213>日本血吸虫(Schistosoma japonicum)<400>39MMIDTSTNMKLSFINRSTQVTEVLPTCLVDAQSYTRQISETFDDIMNQLETYKNTPFIDL60DDVQLTDNKSLDHLIKESFTCSICRGLLVRARLLPCGHHFCRECLYYWFKIRHACPLCRT120CIRTNVSALHVDNFLDELVDRGCNEALKCQRKQRKAEQTSAILDCKYDRRNSENLLRTVV180ESYVQTTAYVDSTQSNVWTNSS 202<210>40<211>84<212>PRT<213>日本血吸虫(Schistosoma japonicum)<400>40MATRTQDFGIDVAISRKLNSRYDPDAERQVIGWIQQLTGQNIPLGRENGQRTLENGRNIE60LNLSNAGGMNVHRNLASKKHKTIP84<210>41<211>115<212>PRT<213>日本血吸虫(Schistosoma japonicum)<400>41GGFIGTAAALALGLSQSLYTVDGGHRAIIFSRIGGVQDEIYPEGLHFRYFGRFKFIFFRI60PWFQYPIIYDIRSRPRKITSPTGSKGQLLVLSTLARAIVGFSYNKSVNPHAKIAL 115<210>42<211>127<212>PRT<213>日本血吸虫(Schistosoma japonicum)<400>42MGSKKLRASGKQRGRPAPRETSAKQTSKSFGAKKAHRGSPNPPGTPQHCPGAETSPAGRK60RDKRKDPRQRRPRRPKSRAAEHRIKHTGPPARRRSDQTNHTPDETRDPAARRAEQAAPTQ120PREKNIK 127
<210>43<211>164<212>PRT<213>日本血吸虫(Schistosoma japonicum)<400>43MSTFPRVFFDITIGGQPSGKIVFELFDNVPKTAENFRALCTGEKGYGYKNSTFHRIIPGF60MCQGGDFTNHNGTGGKSIYGNKFEDESFDNKHDKKFLLSMANAGPNTNGSQFFITTAVCS120WLDGKHVVFGRVVEGEDVVTKMEKVGSQSGRTNQEVKIVNCGQC164<210>44<211>189<212>PRT<213>日本血吸虫(Schistosoma japonicum)<400>44AVEIARLSGFPFVKIVTSHKMIGFTEMAKCAAMKKIFDDGAKSPLSVVIVDNIEGLIEYN60PVGPRFSNFIVQAIRDLVSQPLKGGRHMLVLATTSCREALTDQNLTQAFSWHVHVGMLSK120PEHIIAALEEDGRFTPKEQQYIEKSLSGRRLCIGIKHLLDLIDLVSKNDANHRVAAFLAK180LEEVGIVDE 189<210>45<211>313<212>PRT<213>日本血吸虫(Schistosoma japonicum)<400>45MRTIDVSVRSTAEPLLSPNVAGFVCGGISGTVSRTVTAPMDRLKVLRQMDTPEIAGKDMI60ASLRILIKEGGILSLWRGNGVNILKNCPESAIRFGLHGWLKSVIFPNVKGPLSPKDRLLV120ASLAGATSLTITYPVEILKTRMALRRSHETNSILSVIRILYTEGSLRGFYRGYKISMMSY180VPYSGNGGSLCLYEMLKQRYVEYHYSLQPNTDRTIKVHMPAMVTVPLILISCCIPMIITY240PANLLRTRYQASSSPYAAPIIKSMCSIVQKNGFKGLYRGLSASLSKTVPSVCVTYLVFES300TSELLGVPGLGCK 313<210>46<211>76<212>PRT<213>日本血吸虫(Schistosoma japonicum)<400>46MSKVKVGIIGGSGFDDPNLFKQVGIRKVLSVMLHVLYFPGSQCLSKFVSFIIIHIIYYCN60LLATHFLYLDFYLLIK76<210>47<211>114<212>PRT<213>日本血吸虫(Schistosoma japonicum)<400>47
FYYLKMPRFPPYLTEAQEDDLRQIAQAICAPGKGILAADESTATMGKRLQQIGVENNEEN60RRLYRQLLFSADHKLAQNISGVILFEETLHQKSDDGKTLPTFAKRNDTFITPGK 114<210>48<211>73<212>PRT<213>日本血吸虫(Schistosoma japonicum)<400>48MEALCCSPAEFDARELHRAMKGAGTDESVLIEILCTRTNHQIRQIKEAYGRCKYNEIAII60SCELVSFVYTDYT 73<210>49<211>224<212>PRT<213>日本血吸虫(Schistosoma japonicum)<400>49GVIKTAKYLASSMRDGYMIEFTPRSDITVDYWDYTSYDVSEKSHFSKQQEKIEFNYHYNK60SIMIRDIGFVIKVHGHILKSAYISQHGVVYMANEFKTGSSMRSFGIVGAISRKGDFATAT120KNDTFYIIWYDLKICKFDPEILVDLQMRFYKNGIVQIELVHVYGRPTNCNLTMEILDGFC180QMNRDGSVTMVEKKVWKLASYPSSRIFLGNRFEFTPRSNLSWVY224<210>50<211>214<212>PRT<213>日本血吸虫(Schistosoma japonicum)<400>50DFLPDIQEDLSSSSASQTATMKGISNLGTVTSGETTKLDLPSAGATRSMLRLKKLINVKN60TPFQTRDPFSLPVKRLWCYLVRQPDLDDTFLRHIFRKPYLVQSVITPNIKFSLSSLSTTS120GPPELITTPGLAKSTSSSNLKNISSTTLTDIQKSSGKQTRTHQQTQSTNQTAGSSLLLND180AVRLIHKEHDPLIAMCINQDCRASSNHDPIRVES 214<210>51<211>175<212>PRT<213>日本血吸虫(Schistosoma japonicum)<400>51MRHVEYPKVFPGDPPVQCFLPPFWMKMMYPGPKNPPDHVVFKVHPQMTKRDVWQYLEKIY60NVPVVDVRVKMVYHDLEPVDISVEERTQRLNDSTNFPPKPERYEKVAYVRLAPGEVFVFP120DLFKDKRPSDPLDVLDDDFVNEPKNRQGKATSHSSRKLETNKLQPLKGGIKWFGF 175<210>52<211>436<212>PRT<213>日本血吸虫(Schistosoma japonicum)
<400>52SSPTGVSVELPMDDGNEVIVLRGKPEDLGRALSMVYERAQSTIKEEIEAPNRFHRLLIGR60GGSKLTELLEGYKRVQVNFGENTDRISVEGPSEEVEVIVERLKSRLAELQATVAMTTVKV120DPKYYRHIIGKQGATIGRLRDYKVRVRLPDPDRGDSFACDEIVIEGDPVGVEKAKLEIQQ180LVERLENEKCKDVIIDPHIQNLLRSNSIGTTTPYIRTIYDMFPQVRIIWPESDTNDSMFE240ENPTKSIVQLRGDRQQVDAASEKLQKIIKLVKEENYRQEIHIFKEIRSSFLGREYPRVRK300ILEDTQTRLQHPNPGSNSDIFTVIGREENVCKAIVQLEELQKKLASVKEVIVSIPSALTT360KFAGDQAPSLRSICEQCEGVHIRFAHNKNSKGQSHKLSHIDIVVLGPEKEVNQACSLLSE420LNAKVSELCARGCCSC436<210>53<211>237<212>PRT<213>日本血吸虫(Schistosoma japonicum)<400>53MASISLLNPKAEFAKAQHAFGINLAAAKGLYDVLKTNLGPKGTMKMLVSGAGDIKITKDG60NVLLNEMQIQHPTASLIARVATAQDDMTGDGTISNVLLIAELLKQADVHTSEGLHPRLIT120EGFDIARNKCLEILSKCRIDCPSEMPDRAILISVAATSLNTKVHSDLANLLTEHVVDAVL180SIRRPNEPLDLHRVELMQMQHKTDMDTTLYKGLVLDHGGRHPDMPKTCTNAFILNVH 237<210>54<211>100<212>PRT<213>日本血吸虫(Schistosoma japonicum)<400>54MVCEKCEKKLGKVITPDPWKAGARNTVSSGGRKLNENKLLTSSKNRFSPYSNTFPRCRIC60AQTVHQPGSYYCQQCAFKKGICSMCGVRLINTTQYNQSAA100<210>55<211>181<212>PRT<213>日本血吸虫(Schistosoma japonicum)<400>55ETYLLEKARVIRQASDERSFHIFYQLLASATPAMQQQLLLNHASSYRFLSNGMIELPGVD60EQQFFRETTDAMDIMGINNEDQNAIFRVISAVLHLGNFEFRQERNSDQATLPDHSVAEKV120SHLLGIPLNEMVKAFLKPRIKVGKDLVSKAQTKTQVEFAVEAISKSIYERLFIWLVARIN180K 181<210>56<211>222<212>PRT<213>日本血吸虫(Schistosoma japonicum)<400>56LTSQKALLLFITFILLQYDTECRKSLLNVEDPETWRKLADEKFNKFEKSLYYSLTKRPKN60
VIIFIGDGMSLNTVTGARYLKAENMDLLGGDVQLVWDDWPVASLVRTFNSDRLTTDSGSA120ATAFLSGAKGPDGTVGITGTVKCCKCTELRDLERAKSSLKYASNAGLSTGIVTTTRVTHA180TPAAAYANLLHRDWESNAEISDSGFNCSDAAAQLIANASDIN 222<210>57<211>211<212>PRT<213>日本血吸虫(Schistosoma japonicum)<400>57VKIPIIGIDLLSHHNLLVDASKKRLVDSSTKLYVRGTTATGCSLCPLRFKHLINPYFQPI60LDKYPDLNKTISKLSCVTSNVKHHITTTGQPVFSRARRLAPEKSRIAKNHFDHMIDLGII120RPSSSPWSSPLHMVPKKDSNDWRPTGDYRRLNAQTTPNRYPLPHIHDLTATLKGMTVFSK180IDLVKAENGYCNPVRSLRIPPNALRLEECST 211<210>58<211>239<212>PRT<213>日本血吸虫(Schistosoma japonicum)<400>58IFILSSAFLFALGPMASPQMPIKFQEHLQLVSVGIQPSSIGFSTLTMESDKFICVREKTG60DTSQVVIIDMNDPMNPTRRPISADTIIMNPVSKVMALKAGKLLQIFNIELKSKMKTYTMP120EDVVFCKWISVNTIALVTGTSVYHWAMDGDSNPVKMFDRHQSLGNTQIISYRCDLSQKWL180LLIGIIAQDNRVVGYMQLYSVERKVSQAIEGHAAAFARYVPEGNTNPTTLSVLRRATHR 239<210>59<211>228<212>PRT<213>日本血吸虫(Schistosoma japonicum)<400>59MGQRMYLPLAEKMLIREAEGGKMSRHSELQVLIDLLTRLHKYEEAYKLLNRKDITDHIDA60EDYICDYSYTKLSLLAHLGMWDDLYELAKSQTEINPEDWTPWKKLIEASLEDIQRVEKVM120SLINTVITNHPSARGPHLARLDLYANTLNLGIHLSCNYDPLKLMLDYFNIFCHKPICSMD180LAYLVRMVLPNSDDQIKYVSLIMEIAETGLDLQTMRIRQFIVFYVLSK228<210>60<211>224<212>PRT<213>日本血吸虫(Schistosoma japonicum)<400>60MDHMPLSELSYHLTRDPLSYRTDFEAQLENFNTLKQSFSSAPSQYISRLEDLLSFISQAV60RFYPQHVVEFATGVIQTLLSRSFGMHPEMRMAFLRAFMRIRTRNLISATQAVDVAFKLHR120CRDKQVRKTLRHFLVSDIKRMNKSQKQTKANAIILSFLSKMIKDNSSTVAREAVVTLLCL180FKKNVWNDARTANVIADSCLMSNKKVYVPAIQFFLGKSKALNEM224
<210>61<211>158<212>PRT<213>日本血吸虫(Schistosoma japonicum)<400>61PFLTYEEIEQAEHDGVQFAKEALSNKTKKPKSDSPEFVLNEMSHGKATYLQAFESLCHYS60NIPCVTVKGLAKGVDYVVGMRLNDINEQSQQTSSVMHRLQHAWNAAYLDNKWALFDPMWA120AQRLAVSANTRLSQLVQTGQMDYETDMFYFNGDPAKFI 158<210>62<211>212<212>PRT<213>日本血吸虫(Schistosoma japonicum)<400>62RHQQLAQNEVAQQYLFDASEAEAWMGEQELYLMGDERAKDEQGANNAIKKHEQLQKTVEN60YASEIRGLGERCQALLEANHPESEAVAAKQARIDKMYAGLRDLCGERRSRLDEILKLYHL120LRDILDLEAWIAERILIASSHEVGTDYEHCCLLRERFAEFSRETNELGKYRISAANELCN180ALIGQGHSEAAEIASWKDRINEAWADLLELID212<210>63<211>276<212>PRT<213>日本血吸虫(Schistosoma japonicum)<400>63MLAQAYECVFLKAEKDEKKPAILARISSTLTNLYEDCLVKCSGGAKNVVPKEWPGVLSMK60KGLYEALTQYYQSKDCCESKQYGEQVARLSLAYELIKGAARNSCFPRKYLVEQLKHEEAV120ATKDNDHIYHDNVPTKLSLPAVQSVDVIKKTPLAFPLSSSQNKDYFEELLPIAVTEAVNT180SKGVRASLIDGEICKLQEASAKVNDALASYNLPAALQAKESNSILDSILSKAVSIRHEGG240AEKLYQQLMSIPECVQRNKEIIEAERSALDDEREVR276<210>64<211>225<212>PRT<213>日本血吸虫(Schistosoma japonicum)<400>64VDKVGSIPEEVGRDAKAVAALQRKHHTFECELARLGSRVEGIVETANTLLPSYAADKERL60ICDRRDEVIHAWRQLQCATEQRKIHLHDASDIHRFFSMVRELRLWMETIRMEMTTKEKPR120DVSGVELLMNSHRSLMAEIDAREENFSICLSLGRTLLNRRHPREEEVRKKCIQLVTEKIL180LNDQWAERWETLGLLMEVYQFARDAEIADTWLVSQEAYLNNTDLG 225<210>65<211>296<212>PRT<213>日本血吸虫(Schistosoma japonicum)
<400>65MIGLVYLLLEYCPFLMESAKEIGPSSLQHFLSQSIASWDPEHRVNEVPASNDCLLRITKD60LRSICNDPAPGICVVPDNEDMTKIYALITGPFDTPYEGGFFLFLIRCPPEYPLTPPKVKL120MTTGNGTVRFNPNFYSNGKVCLSVLGTWQGPEWTPAQSLSSVLISIQSLMNDKPYFNEPG180FNIERNPGDAQQYNAIITHETIRCAVCDVLEGRTIFPPDLYAVVESSFEDYYEYYTSVCE240RNMHLSGQPMVDPFEDGRGIFDYANLLKRLKALNNKVKQKHSGINEANPMSIRESK296<210>66<211>190<212>PRT<213>日本血吸虫(Schistosoma japonicum)<400>66ERKSTNKYYPPDFDPNRHRNLNSYHGTHALGQRGKKADQGVITIRFEMPFNCWCLTCKNP60IGMGVRYNAEKKKVGMYHSTPIYQFSMPCHLCAGTIVMQTDPKNFQYVILEGARRKVQKW120DSEENEQVLIPNHFEKKKLSTDAMYHLEHDVTDKKKALEVLPAIQEIQTDRLAHEDDFAL180NQIARKRFRV 190<210>67<211>209<212>PRT<213>日本血吸虫(Schistosoma japonicum)<400>67MSSFRMAGRVVGQLSRINLCAFKQFGDRWISTTSALKAAVPEFSSILEERILGQTLQTNL60EETGRVLSIGDGIARVYGLKNIQAEEMVEFSSGLKGMALNLERDNVGVVVFGNDKLIREG120DIVKRAGSIVDVPVGEELLGRVVDALGTPIDGAGAINTKTRQRVGVKAPGIIPRISVREP180MQTGIKAVDSLVPIGRGQRELIIGRSSDR 209<210>68<211>352<212>PRT<213>日本血吸虫(Schistosoma japonicum)<400>68MKFRIKTNDPGCIEYFSNIVQMVAGLAKNVILHLNKGQLSFVIKERVVFGGITAWCDLEH60AVLFSERICEGISPDQDEIFLELIMDHLLHCIRSNTLSTSNHNSLYSSSSTSNSCTTMNK120SRIGSNSLTGNFDPSICRLLGLKIKLVRRKTPCLAIELEQSSVTGRSRSVWHFIPVQVVP180PRLWDEFIEPPDPDFDVSIFFPPVKTLRPFVDRMKKFAKFVIISANGSGELNFSINVESL240ACVKLTFRGLKARSWMLQESLLSEINDSMDEVRSTVWDSNDVDLDRSKQINDDPNKKLVS300VSLDLRRISQLLSSPKIVPSCFVCNIIHEKLAQFLMLFDNHKLKFNIPASNL35权利要求
1.一类日本血吸虫表皮抗原基因，其编码具有日本血吸虫表皮抗原蛋白质活性的核苷酸序列，所述的核苷酸序列包括(a)SEQ ID No.1～SEQ ID No.34所示的序列；(b)SEQ ID No.1～SEQ ID No.34所示的序列中每条序列的互补序列；(c)与SEQ ID No.1～SEQ ID No.34所示的序列中每条序列有至少70％同源性的序列，及(d)上述(a)～(c)中一条或数条的组合。
2.如权利要求1所述的一类日本血吸虫表皮抗原基因的核苷酸序列，其特征在于所述的核苷酸序列包括具有SEQ ID No.1～SEQ ID No.34所示的序列。
3.一类日本血吸虫表皮抗原蛋白，包括(a)具有SEQ ID No.35～SEQ ID No.68所示氨基酸序列的蛋白；(b)由(a)的蛋白发生一个或几个氨基酸置换、缺失或插入而形成的具有(a)蛋白相同活性的蛋白。
4.如权利要求3所述的日本血吸虫表皮抗原蛋白，其特征在于，其具有SEQ IDNo.35～SEQ ID No.68所示的氨基酸序列。
5.一类抗体，其特征在于，所述抗体能与权利要求3所述的蛋白特异性结合。
6.权利要求3所述的日本血吸虫表皮抗原蛋白在制备血吸虫疫苗中的应用。
7.权利要求3所述的日本血吸虫表皮抗原蛋白在制备防治血吸虫病的药物中的应用。
全文摘要
本发明公开了一类日本血吸虫表皮抗原基因及其编码蛋白，还公开了与该日本血吸虫表皮抗原蛋白特异结合的抗体。此外，本发明还公开了此类日本血吸虫表皮抗原蛋白在制备血吸虫疫苗中的应用，以及在制备防治血吸虫病的药物中的应用。运用本发明的日本血吸虫表皮抗原蛋白，可研究血吸虫治疗药物的作用靶点，从而在开发研制预防或治疗血吸虫病的药物过程中发挥重要作用。
文档编号A61P33/00GK1986802SQ20051011172
公开日2007年6月27日 申请日期2005年12月20日 优先权日2005年12月20日
发明者刘锋, 韩泽广, 王志勤, 胡薇, 冯正 申请人:上海人类基因组研究中心, 中国疾病预防控制中心寄生虫病预防控制所