日本血吸虫表皮抗原基因、其编码蛋白及应用的制作方法

专利名称：日本血吸虫表皮抗原基因、其编码蛋白及应用的制作方法
技术领域：
本发明涉及生物基因领域，具体地涉及一类日本血吸虫表皮抗原基因、其编码蛋白，还涉及该日本血吸虫表皮抗原蛋白的应用。
背景技术：
血吸虫病由血吸虫的感染引起，在世界上有76个国家，受感染者约1.5亿。致病的血吸虫有3种，既日本血吸虫(Schistosoma japonicum)，曼氏血吸虫(S.mansoni)和埃及血吸虫(S.haematobium)。在我国流行的是日本血吸虫。血吸虫病的流行给人民身体健康和经济发展都造成巨大的损失，因此，防治血吸虫病、开发研制治疗血吸虫病和抗血吸虫的药物具有巨大的社会和经济效益。
研制新的治疗血吸虫病和抗血吸虫的药物，首先必须找到合适的药物作用靶点。血吸虫的表皮是宿主和血吸虫相互作用的界面。而组成血吸虫表皮的蛋白质暴露在这个界面上，对小分子的化学药物和大分子的抗体药物而言都是最理想的药物作用靶点。已经有研究表明治疗血吸虫的化学药物——吡喹酮的作用靶点就是存在于血吸虫的表皮。另外一些已知的血吸虫表皮抗原蛋白，例如GST28，paramyosin，已经用于疫苗研究和应用。我们通过大规模的蛋白质组学研究，发现了50个日本血吸虫表皮抗原蛋白质，这些蛋白质，都是可能的药物作用靶点和候选疫苗。
鉴于日本血吸虫表皮抗原蛋白的重要功能，研究和开发日本血吸虫表皮抗原蛋白具有重要意义。

发明内容
本发明要解决的技术问题之一是提供一类日本血吸虫表皮抗原基因。
本发明要解决的技术问题之二是提供一类日本血吸虫表皮抗原蛋白。
本发明要解决的技术问题之三是提供上述日本血吸虫表皮抗原蛋白的应用。
为了解决上述技术问题，本发明通过如下技术方案实现在本发明一个方面，提供了一类日本血吸虫表皮抗原基因，其编码具有日本血吸虫表皮抗原蛋白质活性的核苷酸序列，所述的核苷酸序列包括(a)SEQ ID No.1～SEQ ID No.24所示的序列；
(b)SEQ ID No.1～SEQ ID No.24所示的序列中每条序列的互补序列；(c)与SEQ ID No.1～SEQ ID No.24所示的序列中每条序列有至少70％同源性的序列，及(d)上述(a)～(c)中一条或数条的组合。
较佳地，所述的核苷酸序列包括具有SEQ ID No.1～SEQ ID No.24所示的序列。
在本发明的另一方面，提供了上述日本血吸虫表皮抗原蛋白，包括(a)具有SEQ ID No.25～SEQ ID No.48所示氨基酸序列的蛋白；(b)由(a)的蛋白发生一个或几个氨基酸置换、缺失或插入而形成的具有(a)蛋白相同活性的蛋白。
较佳地，其具有SEQ ID No.25～SEQ ID No.48所示的氨基酸序列。
在本发明的另一方面，提供了一类能与上述日本血吸虫表皮抗原蛋白特异性结合的抗体。在本发明中，可以使用一系列本领域已知的方法来制备针对日本血吸虫表皮抗原蛋白的抗体。例如，将提纯的日本血吸虫表皮抗原蛋白产物或它的抗原片段注射入动物体内以产生多克隆抗体。同样，表达日本血吸虫表皮抗原蛋白或它的抗原片段的细胞也可以用来对动物致免疫而产生抗体。本发明制备的抗体也可以是单克隆抗体，这些单克隆抗体可用杂交瘤技术制备。
在本发明的另一方面，提供了上述日本血吸虫表皮抗原蛋白作为免疫原，在制备血吸虫疫苗中的应用。
在本发明的另一方面，提供了上述日本血吸虫表皮抗原蛋白在制备防治血吸虫病的药物中的应用。应用本发明的日本血吸虫表皮抗原蛋白，可研究血吸虫治疗药物的作用靶点，从而在开发研制预防或治疗血吸虫病的药物的过程中发挥重要作用。


图1是本发明日本血吸虫表皮抗原蛋白的免疫荧光抗体原位杂交实验结果示意图。其中，T代表tegument(表皮)；G代表gut(肠道)；ST代表subtegument(下表皮)，绿色荧光部分是Immunophilin的信号。
具体实施例方式
下面结合附图和实施例对本发明作进一步详细的说明。应理解，这些实施例仅用于说明本发明而不用于限制本发明的范围。下列实施例中未注明具体条件的实验方法，通常按照常规条件，例如Sambrook等人，分子克隆实验室手册(New YorkColdSpring Harbor Laboratory Press，1989)中所述的条件，或按照制造厂商所建议的条件。
实施例1 日本血吸虫表皮抗原蛋白的分离和鉴定实验1.日本血吸虫表皮的分离纯化血吸虫表皮的纯化按照Triton-x-100提取方法进行。表皮抗原蛋白从血吸虫雄虫、雌虫、雌雄混合成虫和童虫分离。虫体用Krebs’-Ringer’s，Tris-马来酸盐-缓冲盐液(KRTM)洗3次，再用KRTM/0.2％Triton X-100溶液悬浮。这个液体和虫体的混和物置于冰水浴中，在摇床上摇动10分钟。然后，装有液体和虫体的混和物的试管在涡旋混合器上混旋几次，然后再放回冰水浴中，静置使虫体下沉。用吸管吸出上部液体，放置到另一个新管中。这个试管在离心机上用2500×g的离心力，离心15分钟。得到的沉淀就是血吸虫的表皮。Krebs’-Ringer’s溶液的配方是0.15MNaCl，6.2mM KCl，1.2mM Na2HPO4，1.2mM MgSO4，10mM Glucose and 20mMTris-Hepes，pH7.4。Tris maleate缓冲溶液的配方是0.2M Tris and 0.2MMaleicanhyclride，pH7.4。
2.日本血吸虫表皮抗原蛋白的电泳分离日本血吸虫表皮用裂解剂裂解，蛋白溶液用SDS-聚丙烯酰胺凝胶电泳分离，条带切割后再做第二维的在线毛细液相色谱分离以及质谱鉴定。软件分析得到鉴定蛋白。分离好的血吸虫表皮，用SDS-聚丙烯酰胺凝胶电泳(SDS-PAGE)缓冲液悬浮，用超声波促进组织细胞裂解和蛋白溶解，超声波裂解方法为超声1秒，暂停3秒，总时间2分钟。并用100℃加热5分钟以使蛋白质变性。然后这些表皮抗原蛋白混合物用SDS-PAGE分离，分离方法按照普通的SDS凝胶电泳方法进行。胶上电泳泳道用刀切割下来，再用刀均等分割成8-12份。接下来做蛋白的胶内酶解。
3.日本血吸虫表皮抗原蛋白的胶内酶解过程胶内酶解按以下方法进行蛋白胶块用刀切碎，每个小块大小约为1立方毫米。加入100mM NH4HCO3，洗2次；转入到试管中；加入10mM二硫苏糖醇(dithiothreitol，DTT)/100mM NH4HCO3，56度放置反应1小时；冷却到室温，离心吸去DTT；加入等体积的55nM碘乙酰胺iodoacetamide/100mM NH4HCO3，室温/黑暗反应45分钟；吸去iodoacetamide，加入100mM NH4HCO3，洗2次；去掉NH4HCO3，加入乙腈缩胶；去掉乙腈，真空抽干；加入100mM NH4HCO3，胶膨胀，去掉NH4HCO3，加入乙腈缩胶；去掉乙腈，抽干；加入含Trypsin的消化缓冲液(50mM NH4HCO3/12.5ng/ul trypsin)冰上放置45min；去掉多余酶液，加入50mM NH4HCO3，以刚覆盖胶为准；37度，过夜酶切；酶切结束后，从试管中吸出上清液到新管子中，胶中加入60％ACN/0.5％HAC，在水浴中超声处理15min，再重复一次；吸出上清液到新管子中，在原管子中再加入60％ACN/0.5％HAC，37度40min，吸出上清液，与前面的上清液合并，真空抽气去ACN。
4.日本血吸虫表皮抗原蛋白酶解肽段的在线色谱分离和质谱鉴定酶解产生的肽混合物，用真空将溶液体积抽减到30μl。之后，每个组分的肽混合物上样到一个反相捕获柱(C18，5μm，300，300μm i.d×5mm，LC Packings)，在20μl/min的流速下脱盐。然后这个捕获柱和一个分析用的75μm×150mm C18柱(LC Packings)相连接，肽混合物就在200nl/min的流速下，从这个柱子上洗脱进入装有Protana NanoES的电喷雾离子源的QSTAR pulseri中。Agilent 1100毛细液相系统(Agilent Technologies)用来提供流动相A(0.5％乙酸/水)和流动相B(0.5％乙酸/乙腈)，线性梯度是60分钟内5％B到50％B，30分钟内50％B到90％B，然后是在90％B停留15分钟。在分析柱后通过一个不锈钢的两通(Valco Instrument)连接一个10-μm直径的PicoTip纳喷雾头(New Objective)，加上2500-3000伏的喷雾电压，以获得稳定的喷雾。MS/MS串联质谱谱图通过信息依赖的采集(IDA)和任务-循环增强来记录。在每个测定扫描中，选取3-6个信号最强、带2到3个电荷的离子，用滚动碰撞能量打碎，以获得串联质谱的信息。
5.日本血吸虫表皮抗原蛋白的软件搜索鉴定用牛胰酶的自裂解片段如([M+H]+)1153.5741，2163.0569和2274.1600作为内标，或者以预先数据库搜索得到的得分高的肽段的分子量，来校正所有的质谱谱图。质谱搜索用的数据库，包括从8420个从EST cluster预测得到的血吸虫蛋白质序列，以及可能的污染蛋白质序列如牛胰酶的序列、宿主蛋白污染如人蛋白质序列、小鼠蛋白质序列、大鼠蛋白质序列、兔子蛋白质序列和角蛋白序列等。搜索软件是MASCOT(http://www.matrixscience.com/，Matrix Science)。搜索参数为最大漏切点，3个；monoisotopic；肽电荷数，+1/+2/+3；修饰，半胱氨酸carbamoylmethylation和methionine氧化。搜索结果合并后，用下列标准进行过滤筛选1.对每一个串联质谱谱图的搜索结果列表，只提取排位在第一个的结果，其他的则丢弃；2.搜索得到的肽段中如果有KR，KK，RK，RR等序列，则这个肽段丢弃；3.搜索得到的肽段，长度在8个氨基酸以上的保留，小于8个氨基酸的丢弃；4.重复打到的肽段，只计算其中分值最高的，把这些肽段分值加起来，做为蛋白的得分；5.蛋白的得分超过60，就认为是可信的结果。低分的蛋白，手工检查后保留或者丢弃。匹配到宿主或者牛胰酶、角蛋白的肽段，则丢弃。
实施例2 日本血吸虫表皮抗原蛋白的生物信息学分析和功能注释1.同源性和相似性分析日本血吸虫表皮抗原蛋白序列对GenBank(http://www.ncbi.nlm.nih.gov/)的非冗余数据库(Non-redundant，NR)做BLASTP比对；日本血吸虫表皮抗原蛋白序列对Swissprot数据库做BLASTP同源性比对。根据比对结果得到的与已知其他物种或已知的血吸虫蛋白的序列相似性，做出日本血吸虫表皮抗原蛋白的功能注释。
2.文献查检根据已经发表的文献报道，发现鉴定得到的24个表皮抗原蛋白在虫体上的位置，有一些的确在血吸虫表皮上。而其他则是新发现的表皮抗原蛋白。举例说明，比如以下表皮分离和质谱鉴定得到的蛋白，根据文献报道，就是已知的表皮抗原蛋白，而且有些蛋白已经有进行了疫苗和抗原研究Paramyosin，tropomyosin，myosin，dynein light chain 1，22.6 kDa tegument membrane-associated antigen，tegumental antigen Sm20，nitric oxide synthase 1(NOS1)，leucineaminopeptidase，14-3-3 proteins，SnaK，cathepsin B-like cysteine proteaseprecursor，21.7 kDa antigen，FK506-binding protein 50(immunophilin)。
实施例3 日本血吸虫表皮抗原蛋白的免疫荧光抗体原位杂交实验为了进一步用实验的方法验证用蛋白质组学手段鉴定得到的血吸虫表皮抗原蛋白，选取了一个Immunophilin(SjFKBP50，GenBank登录号为AY815389)做免疫荧光抗体原位杂交做蛋白定位分析。
实验方法血吸虫成虫用冷冻包埋剂(OCT)完全包埋，切成5μm厚的切片。用抗Immunophilin的抗体覆盖切片，在37℃反应60分钟。该抗体是用重组表达的Immunophilin免疫小鼠制备的单克隆抗体。随后切片用PBS洗涤，再加入FITC-偶联的兔抗鼠免疫球蛋白的抗体(1∶40稀释，用含有0.5mg/ml的Evans蓝的PBS稀释)，置湿盒中37℃反应60分钟。染色结果用Leica DM-RB型荧光显微镜观察并拍照。
如图1所示，该实验结果证明了Immunophilin确实分布在血吸虫成虫的表皮上。这说明前面表皮分离和质谱鉴定的结果是可信的。
序列表<110>上海人类基因组研究中心<120>日本血吸虫表皮抗原基因、其编码蛋白及应用<130>NP-10278<160>48<210>1<211>725<212>DNA<213>日本血吸虫(Schistosoma japonicum)<400>1cagcaaacgt gcttgaaatg ttttagtcaa caattaccag atatgtgaat caaatgagta 60gtttagaggc tagccaaatt ttggcatcag ctctaaaaga tgtggatgaa attctgaagg120tgagtaattg tgaatcgaac gatatttgca acgcttacga taatcctaat aatcagtaca180cgttgccaga aaagtcagtt caaaatgttc agaggcaatc tcatagtata aatgttgaca240atagcacgac gtgtgatgag acaggtcact ataggactga aaaagagttt ttcactctta300tgaatagact tcataaatgc atagagaaac tacatctttc accggacatc actccacttc360atacaccgga atattacccc tcgaatttta gagtcactga aaatggtcat ttcaacctag420ttgaaccaac tatcacacaa ccaacagatt gcaatttgat taataacaaa caactacagc480ttcaacaaca acaaccacag catatagtgt tgtcgaatga ttatacaaat ttgaatgata540aacttcttca tcaacgaatc attcgtcttc aagctcaaat acaagcacaa gctaaccgta600tagcagagat gaagatggat aaaaccccat cgtctcattt agtcaatcgt accaacagaa660ctgatcaaaa tgatacaatg aatcaattta ccattgatat ggataatcgt cagttattga720acgaa725<210>2<211>1480<212>DNA<213>日本血吸虫(Schistosoma japonicum)<400>2attattagca tcaatgtttt tagtatttta ctttacgtga gcattttaga gcttgttaca 60tgctagtaag taattttcta agttatttga aatctatata tgagtcactt gttttaagat120tattgataat aaaattaatt ggaatcctta gaacattaac aagagtaaag tcaattgcca180gaaatttact gtattcaatt aatattttgt tattttgata tcgcaaatca attcgatcta240gataatcagt agacactgga taattgtttt gtataagtat ggggcacttc agcatttcgc300ttctacgaat ccaacaagga tcgaaaacag aaccatctga cttcgtggtg aacaatttac360cactatacca ctgagctgga tcgaaacagt tggtaattct aactaagtaa ctgcctcata420ccccacacga caaggcatcc gctgtggagc tagaaggtcc tgtgttcaac cgttggtgag480gccatggatg cccactgtca aagagttctg tgtttggaca aaacggctgt ccggtgcttc540
ttgattgtca gtgcttgtcg aaaggagatt aatttgtgta attctattaa attagacagt600gaactgagta gattcttcac aagtctttat gatgtagacg aaaatgcagt aaacagtggt660atagattata ggttgaattt acaagggaag ctaacgagag caggggatat tgtggaccta720gcatctaaac caatgtttga atatgttaat gaagatatat ttaagaagag acccactttt780acaaaattta tttctctact ggacaactat aatcctaaag ttggcgtaac tgaaattgtt840acacaacaac agcaaaatga agaaaacgaa ttcatcaatg aattactcaa gacatctatc900atgaagatga ctcatacatt tcttgttgag aaacaaaagc tgtcaggtga tattaatgat960tttggaaaat acttgaagga attatggttt agacgatatc aacgtagaag cccaggagat 1020tcttctgcat tcgaacatgt gtttgttggt gaacataaag gtcttgaagt acttggatta 1080cacaattgga ttcaatttta tttgaaagaa aagaacaatc aattgaactt ttttggatgg 1140actaaaagac tctgtaatga tcagcttatc actgtatcat atgtcaatga aaataaatat 1200aaaaagccaa tgggaagttt ttttgttgga tcatcaccag aatttgatat tgctgtctac 1260acagtcgcat ttgcattaca tcagaaatct gcattcgaag tgaatattgc tggatgtaaa 1320gtacgcataa cttgtcatac attatcatct tctgaaatgg gtacttgtta tatgggttaa 1380gcaaaactca tttaaatact tgttttttgc ttgaaaatga aagaacattg aatttatttc 1440aacagttcat ctaataaata aaaattttag aaactaaaaa 1480<210>3<211>838<212>DNA<213>日本血吸虫(Schistosoma japonicum)<400>3cgaccctcaa ggcattatta tcctcatggt tctaagcagt cagtagttgt cactttacat 60atccataaag caaaactctg tcttgtaact agcataaagc ctacgcgatg agtgaaatgg120gatccggata tggttcacca caaattcaac gaaggtattt aattcctctt gctgaacagt180ttgtgagaat ttctatagat tcttcatctg tggatgaatt tgtagaatac ataattcaaa240acaaatccac aagttgtagc agatctaata taaaactatg gcgcaaagat gcgtgtaaag300cgctaaggct agcaaacacc cgtacaatag tcgctcctgt tccaaaagat tcacctgtca360cagttgacgg ttgcgagtct tctaaagtag atgcagacag aaggttccca cggaaacgtt420ctaagttgag tttagagcca ccagccgaac ccatctcccc tcgaactgct tcctgcacgt480tatcgaatat cagatgtaca gtccctatgg atttacaaaa acaaataaaa ggctttctca540cttgtcacct gctttccgac ttaggattac cagtttggcc gacattgttg gttaacggac600ctccattctg cggcaaaact acattaattg aggcagcatg tggaactcta ggattaaaaa660ttatcactgt gtctgtcggt acagtaactt cgtcgatgcg ttcgtggatg gatatattca720atcaggccaa agtttcctca ccgtgtattt tacatttgga tgatattgaa agcatggaaa780aacactcttc tcctgttgga gccttccgtc ttacttgcct tctaaaaagt catctact 838<210>4<211>1445<212>DNA<213>日本血吸虫(Schistosoma japonicum)<400>4ctggtatccc cggtactgga aaaactgctt cagttcaagc cgtattatct actatgcata 60aattagttgc tgattcatgt ttagaaagtc aattacctgt atttcaaaca atttatgtta120
atggcatgcg tgtcagtgat cctaaacaga tttacatcca gatatatgaa caattaactg180gtttgattgc aactacaaag tctgcgtgtg atttattaga aaaggaattt tgttctagta240caaataaaaa attaaatcat cgtgaagttt ctgagaaacc tgttatttta gtcatcgatg300aattggattt attatgtact cgtcgacaag atatacttta cagccttttc gatgggccca360cgcgccataa taaccgtcgt gttttgatag tattagccat tgctaacact atggatctac420cggaacgttt gcttcatcca cgagtagcaa gtcgtttagg cttaactaga cttactttcg480caccttattc ccatgaacaa ctttctcaga ttgtgagaca tcgtttatca tctttgtcaa540acatacttca gcctaaagca cttgaattag ctgcacgtaa agtggcggct gtctctggtg600atgttcgacg cgctcttgat atatgcaaac gagctgctga aatagtttca tctagtgaaa660agactaacaa agaaatagat atttcgcata taaatgctgc tctaaaagag atgttcgtta720cacccaaatc agatgcaata tgtgcctgtt cattgtacga aaaattgttt cttcgtgctg780ttatagccga atttcaggcg cgttcaacag aagaagctcg attggatcga tgtattcgtc840aaatgtcagc actttgtcga cttgaaggtg ttccatgccc aactacatca gaagtatttg900ctatttgtgc tagtcttggg gctcacaaat tattgctgac ggaaaggtcg cgatatgata960ttgctatgct tgttcgtctc aattgtacaa agtctgatat tttatactgc ttaaattcac 1020aatcaaatgg ataaatgcaa gataaatggc tttgaagatt tacccattca tttgtacttg 1080agactatatt gtttttcatt tcaaaaacca tacatggata tgtgtgcatt tagataaatg 1140tttcgcattt tgtttaattt ctgttgtctc aaaccttact attcatggaa atcgaaattt 1200ttaaatgttt ttataaatca ctttgtcaat aaattgcaag attactattt ttgtattatt 1260tctattatat ttgtagataa aaactgattc ctatgtattt taaaatgctt cctctatatt 1320tcttaaagtc actttctatt gcatctttta gaaatcttct tttattcatg atttattgat 1380attctagggt agcttttaca atataaaatt aatattctat tgaatacatt atctttctaa 1440tagat 1445<210>5<211>533<212>DNA<213>日本血吸虫(Schistosoma japonicum)<400>5attacattca atcataaatc tcaatgtttc taagtgtatt tcttatctgt aacatactac 60tatgtacgac gtcgacattc aagttggtca atgaaagaag tacaaatgaa gaagatcaca120gtgattcaca agcccttaag ttatctaagc ttcaaagtga agattatgcc cctagatatc180tggttccaga catagaagaa ttcaatatct catcaccatt cattaatgaa caattacgta240atcaaatgga tattttaaaa caagaattag aagaaattgc caagaaactt aatcatgtac300cgaattcatg taatagtaac ttatacaatg gattattagt tagtatatgc attggattat360tcagtattat aacattggta taaagtaata acaattacat tcataatata actgttactt420atttattaca cacatactac ttaaatagta ataaagatct actgaaatca atcatttgtc480attatcattt tgttcatgtg ttttttatct gaataaatat catgagtatt gat 533<210>6<211>1410<212>DNA<213>日本血吸虫(Schistosoma japonicum)<400>6
catgaatgga aaggtgcatg gtgtgatggt gcaccacaat ggcgtgaaat aagtcagaaa 60gaaaaggaaa atatccactt aaacttcact gaagatggag agttttggat gtcatttgaa120gattttgtta catgtttctc aagagttgaa gtatgtcact taggtcttga aagtttagag180tttaatcaag atttccatgg aaaaagacgt ttagaagaag ctatattctc tggtcaatgg240caacgtaatg taaatgctgg tggttgcata aataatcgaa cgacctattg gacaaatcca300caatttctca ttactgttga agatccagat cctgatgata atgataataa atgttcaata360cttgttgctt taatgcagaa agaaacaaga aagaaagttg gtgcagattt tcaacctata420ggttttatgg tttatgcagt accagatgat caaaccactt tgatgtcacg tgcgcaattg480ttaactaaga ctcctatagc taagtcacaa tttattaata cacgtgaagt tgttgcacaa540tttcgtgtac cgccaggacg atatgttatt attccgagta catttgatcc acatattgaa600gctaatttca tattgcgtgt catttcgcaa ataccaatta cagaacaaga actagatgaa660gacaatacta accgaggtct accagacgat atcattgaat ctttgaaatt ggaggatact720ttattggacg aagataaaga aatcgaaatg agatttatgg ctttacgtga tccgaaaacg780ttagcaatcg atgctactaa aatgggcgaa ctactgaata atagcacatt acaagatatg840cctagcttca aagggtttaa taaagaacta tgtcgcagta tggttgcttc tgtagataac900aacttaactg gtcttgtcga gttagacgaa tttatggacc tttggataca agccaaagga960tggaaacata tttttcttaa gcatgatatt gatcaaagtg gatatttcga tgcttatgaa 1020cttcgtgaag cactgaatga cgctggtttt cgtgttagca atcttctgtt caacgctatc 1080gcacatcgat acacagatcc tggtacagat aaaattagtt ttgaagattt tatgttatgc 1140atggttcgac ttaaaacggc attcgaaaca attgaagcgc atccgaaaaa tcttgaaggc 1200acatcacttt tcatgaagga agattatcta cgctttaccg tttcaatctg aatatgtgat 1260agaaggagta acaaactgga gaaaagtact gccaatttac tatccgtttt ctctactatt 1320taaattcatg tgtttaatta tttgtcgaat ttcaaaatat aaaaacatgt cactttgttt 1380attttcatga ataaaatact agaaaataac1410<210>7<211>965<212>DNA<213>日本血吸虫(Schistosoma japonicum)<400>7cgcgtgtaaa taacttgaaa aaatggctgg atctgtatct cgtggcattt tcgttgttct 60tgaaggagca gatcgagtgg gaaagacaac gcaggcatca cttctagcaa aagctttgtc120ggaaatcaca cagaaaaaaa cattgtctgt taaattccca aatcgtgata cacctctagg180acaccaacta gatgcatatt tgggtggtaa tggcgatatt aatagccatg cattacattt240gttgttcact gcgaatcggt gggaaaaaca gtctgaaatt cggaatgcca tttcaaatgg300gatatccgtc gtagcggaca gatatattta ttccggaatt gcgtacaccg ccgccaaact360cccaccaact ccaaactggg aatggtgttg tgaaatggaa aaaggtcttt tggaggctga420tttagtgata tgcttaactc cagaaaatct agatgaatta aattctagga acggttatgg480aaatgagcgt tacgaaaagg acgacttcca aaagcgtgtt ttggagaatt acgttcgact540gtctaaagaa gttgaattgg acaatagtga gtggcaaaat aatgatgaaa ctggtgactc600agtcgggttg tggcatttta tccaagcaac aaataaaact gttgaagaag tgcataaatg660cattatggta ctcgttaaat ctaaattaga atcgataagt ggtccagaaa ttcatgattg720tacaaagaaa aaagattgat cagtttccca ttaatttttg cacaaagtag cacctgatat780ttcttgttct tgccattact gcttctatag taaaagcttt tcaaaatgcc tttgcaattt840
atattcacat ttattaatac agttgtgtaa actaaatcag ttcattttgt tttttatggt900ggcagttgtc attagttcac ataattgtgt cactactgac aaaatcataa attcttttgt960cttgt965<210>8<211>781<212>DNA<213>日本血吸虫(Schistosoma japonicum)<400>8aaaaggtaat actattgaat aaactgggtt aaaataaggc aagttagtct aaactcctgt 60gaaagctcta gttctacatt tatttgagtt tcttttttga gtgtaattaa cttttgtttt120tttggttaaa gaatatacag tgtatgtact actctgaccg ttctatattc attcctcatg180aagctttctg aactcaaacg tgaactaggt caatgtcttg atgagaaaca agagattgca240acagaacgag atatctatcg taataaatgt gataggttga acaatgaatt gaactatatc300cttagtggag atggacgaaa aattcaagat attgacactt taatggctga aagcagaatg360ttaaaagatc agattgaatt gttgcaaatg gaaaaatctt tagcgctaaa tacagtaact420aaatacaaga agcttttgga tcgaaaacta tcaaaacatt catcatcagt cagcagtgaa480agttgtataa ctccagcaat ttcacctaag catcaagaga aaacaatctg agaagaagaa540aaagatttaa actgtgatga catggtttca aaacaatttc cagttgtgtt ataacttatc600tacattcatt atgtgatact ttaaaatttt tatcatttca catgttactg tgatatgtaa660ttgtacttat tagtagtatt agtagactta tatgataaaa taactataga ttcatcttgc720agcctatttg aatctttcca catgttcatg tatattccaa taaaaacata tgtatcattt780c781<210>9<211>2428<212>DNA<213>日本血吸虫(Schistosoma japonicum)<400>9ttggaaggac ttcgaggagc agaaggtgaa gttggtgaag caggagaaca aggagaaaca 60ggtttacctg gtgaagaagg tgatcaaggt gaagaagggc cgattggcga aattggtgaa120atcggagaga ctggtacagt aggcgacata ggaatagaag gaaacgaggg tcccctcgga180gaccaaggtt cggaaggaga tattggaatg agaggagaat atggacctac tggagaaaaa240ggtgaagtag gtcttagagg agatccaggc aaagccgcca cagattgccc tattcggcca300aagggtgaac aaggtcgatt aggcccacct gggttacctg gaccacaagg accacctggg360gatcaggggt tacctggact acgtgggaaa gttggtgcac caggggttgg agtaattgga420gcaccaggag aacctggtga tagcgggcac ataggcctac ccggagaagc aggagaacct480ggtgatcaag gtcccgaagg tccatctggt ttggctgctg ttaccggcga acaggggcca540aaaggggaaa gagggatacc aggcgatgtc ggagatccag gtgtagatgg tgttcagggt600cctcctggtt acgacggaga aaaaggcaca cagggaccac agggtacaaa agggacagac660ggagttgatg gcacacctgg acccaaaggg ttttctggag atcgaggtcc atccggtaat720cgtggacaaa tcggtgacca gggagaaact ggcgtggctg gtattcaggg tgttaaaggg780gttcaaggaa ttgcaatagc aggtaaactt ggtgaaacag gtgaccccgg cgaaaatggg840gttacagggg agcaagggga tccaggtcct gatggtgagt taggagaaga cggagagatg900
ggcgaaaagg gagaaaaagg acaacctgga ctattggaag gggagagagg tccagttggt960gaccaaggag aacgcggaga tattggagaa gtaggaccat ctggtccagt gggtgatatt 1020ggccctaaag gtagagacgg tgaaaagggg caaccaggtc agacgaatta ctcctccata 1080ctgtttgctc gtcattatca gacacctttt gttgagaacc tcacttgtcc aggtggaaca 1140aacaaactgt tcactggtta cagttacgtt atgggaggtg gtgtagatga tttagtcagt 1200atggatttag gtacaccaag ttcgtgttta tcaaagttca gttcattacc tatgactcaa 1260tgtgaaagag atacaacttg tcaatcaagt atgcgacatg aacgatctta ctggttggct 1320acattagtac cacgttctga acaaccaatt ccagtgaatc aaacagctga tcaaattgct 1380agatgtgtag tttgtgaagc accatcacat gtatttgctt tccacagtca aggtgaaaca 1440ttacaaccat gtccaagtac ttggactgaa ttatggactg gtgttagttt aattcttcat 1500acttccggtg cacatggtgg aggtcaacag ttatcttcac ctggaagttg tatggaacat 1560tttcgttact caccagttat tgaatgcaat aataatgtag gcatgtgtca ttactggtca 1620gatgcaaaag tctattattt aagagcactc aatccaaaca ttacacaatt tgaaaaacca 1680gtaggctttg ttatgaaagc agctgaagga ccagtactga ataatgtcag taaatgcaga 1740gtttgcatga aaatccctgc ttaaatcaat aagaatttac ccacagatat aatacaaaca 1800aacatatata atcagatatt ttcattgttt taaacgatga acaatcgaaa gtaggagaaa 1860tgaaagtgta tcatcgtctt gaatgaatta ccatcttcaa cattaataag catatatata 1920tatatatata tatatatata tatatatata tatgcatccc tatcctctca atgttttttg 1980tctctctctt ctaaaagttt cttttcacaa acatctattt attcattata atctggttat 2040ttttaaagtt acctttactt catttcatat ctttttgttt aaataggcca ttcaatattc 2100aattcgttta acacttttgt gcattttgat tttcaaaaaa caatacgctt ttttttaaaa 2160aaaaatatgt ttacttattt gtccttgttt aaagtttatt tgttttaatt tttttcttga 2220aaatttaaag cgccatattc agttttagtc ataataagca tgatttatga attctataag 2280agttatctaa aatcttatta tcttttaacg tatatataca tacttacata cagattcttt 2340atgtaatgaa ttatattgat aaattgaaat tgttatacaa atcatgttcc aataattata 2400gtagtattaa taataaaccc tcgtgccg 2428<210>10<211>2097<212>DNA<213>日本血吸虫(Schistosoma japonicum)<400>10gctgataagc gcgatcaact tggggtagat tcccccatgt atcactagaa ttgcaacctc 60aaggcaatca tctattgatc gcatacgccg gaggctgtgt ggcggtagct gttccccaga120ctgttccatc aggaagtgta acgcagccag ccgtttctgt ggatggtgtc actgctccca180cagctgaagg ggaagttgtt actccgagtg ttgatgaatt ggctccaact attactcagg240agacccaggg cgaagctgta ccaagtgata aaggtgaaac tgctggcgaa acgacccaat300caatcttaaa tgacagtgct gtggagtcaa ctcctagcac cccagccaag ggacattctg360ataagaggtc gactttaaag ttcaaggcgt taacgcgtag tttgagacct gatacaccta420aactagagga gcaagtagaa ccgtcattac ccgtacctcc agctcctcgc atcagtcatt480tacttcttcg cgatcaacca gttgagtggg cctcatggcg tgtttcaacc atagattctt540tgtctactga agttgtcgtt gcttatggag atggtgcatt tcaggtttgg cctattgttg600cagctgttag tgatcaacta tttgaaccaa tcattgtatc aatgatcgat ccaccaaaca660ctccatatgg tcctttacct tgtggtgcaa taaacaaaat tttagtcaat ccatctgcaa720
atggtggttt gttaacagtg ttttgtggag gcttaccacg tccccaattt gaaaataggt780atgctgtatc tgtactacag gatcatgaac atcatgtttg ttatcagttt ggatcgaagg840ttattgattt tgtgctcatt ccaagtgaag gaaatatgtc aaatgaaacg gttcccggta900attttatgac aaaaccttct gcacctagtc attctgctac attactagta cttactgaac960gtgagcttgt tgctgttgat ttaacccaac cagattggcc tgcctacgat tcaccttatt 1020tgaattgcat ggatttctct ccggttacag caataactca tattgggcag gttccatcag 1080ctctaataca acgattgcat caggctgctc acacaactac agacgatatt actaattgtt 1140cgtggccaat atggggggga tctcaagctg atggtaacaa aaagaattta gcacaaaatg 1200ctggtaacga tatagtcgct ctaggccatt caaatggatg ggttacttta tgggcaattg 1260gtagaggaga tactacaatt catttaggaa cattaccaac atcatcacta tttaactcag 1320ctgatttaca aaatgaccag aagtgcggca gttcagcatt aggagaagaa acttggccac 1380cttttcgtcc agtcggttat tgttctcgag cacaacgtca tactctcgaa ggttgtgatc 1440cacgcctggc tatttcacag ttacttatat tactcggaag tccaattcga tcaggtgtta 1500gcgattcagt tgcaccagat acactgactc tcgttgttgg tggcgctagt ggtcaagtga 1560gcttatgggt tgctggttca gaaggctttt tgcatactcc agaactcact tcttttgagc 1620ccgacgtgtc ccgtgtttat attaacctca ttgaccaatc tgaagaggat aagtatatat 1680ggaaggatgc tccatgtctt cgacctactg aaggtgtgcc tcaatattgc acaccaactg 1740gattaacatt ccatccttcc catcttgtcc agttaaatcc tcccagtcag atcacatctc 1800ttgcactaga attatcttgg aatctgttag ctgttggctc ttcacatggc tttgctttgc 1860tggacttgtt taatcgttct attattcata cccatttcac ctacgattca tctggtcctg 1920caaagatctt taataccgtg tcaactgtgc aaaatgtaat aatggctcgg gggaaacaaa 1980ttaaatcaaa tataagacat tcatttagac gtttaaagaa ccttcggacc tcaacaacca 2040ccaccgaaag aagtttaact gaacctgcac cagttgatga acaatatacg ccgaatt 2097<210>11<211>1926<212>DNA<213>日本血吸虫(Schistosoma japonicum)<400>11tttagagaat ttactttcag atcattgtga tttcattgtt ctaaatggtt gaccactcgg 60agaaatacta ctagttcgta tggtctttta cagttgtgag actttacttc gatctatagc120gttgtctgtg ctatgtaagc taacatgata tagaggcttt ttcccgtcga tgttactaca180gtgcggttgc tttacttttg ttcaataaat gtgaagaaat atataccagt tttcgatagc240ttaccaccac tgtacaagta tgactatatt gcgaaatatt tttattaata cgtgatcgtc300atgcctaaga taggactggt gactaccatg tttgattata aataacatat gattatgcag360cccaccaggt tttatcattt gctatgatat cacataaaat tttaatagcc agcactgttt420aacctttggg ctacgctaaa gtatagtgtt gattaaaata gatatattgc aatccgaaaa480acaactatct gccgactgtt ttgttaaata aaaacgactt gttctgttta cctatgtcca540atctatgtgt agaacttgat aatagacatt gtaattataa aatattacct tgtttatata600gctaccaggg ggtaaaacta aagtaatttt acgcgctggg actcagaaac catgtgagca660cgaccgagtc attgctgatg tatctcttgt cgttgatgct aaaataattc ttcagaatca720gaaagtcact tttgatgttg gtcatgcaga ggattttggc gtcatgaaag ttgttgatga780tattgtacaa gacatgggtt gtggcgaaca gtgcgaagtg actcttactt ctgattatga840attcacagat tcggagaggt catttttaaa aatcagtgat gagtgtttcc ataagatttt900
gtttaacata tatcttgagg atattcaaaa gcttgcagaa ttttggcaaa tgtctgagag960cgagaagttt attatagctg atgtgatgaa atgtcgtgga aataagcttc taaaagctgg 1020attgctgatt cgcgcatttc attgctataa aaaagccatt agaatcctag aagggagcat 1080cagtgtaact aatcatgact gtactaactt ggcgattccg gattctgaat catcgaaacc 1140tacaccacgt caattactag ctatttgttt atcgaatgca gcccactgcc tgtttcaatc 1200tggcaccaca tgcgatacca atctaaaaaa atcccaattt gactatcttt ctgcatgtat 1260caaatgttgt aaacgagcag tcagtcttga accaaattat atgaaggcgt ggtttcgtat 1320ggccaaaact catgctttat tgggaaacta tgaagaagct attacagctg gagagaaatg 1380tctttctcgt tctaccaatg aaaacatctc tgagtctaag tggattaatg ttgatgaacg 1440cacaaaaacc tatattacca acaaaataaa gacattgctt tcagaatggc gtgtatcact 1500aacaaacgaa gaagaaactg aaaggtcaat aatccgaaaa cgaacaatta agaaatactc 1560atcttttgga gattggtgct gtgatgaatt ggacgaaatt gagaagttgc ctatttctat 1620atggtcgaat gatctagcag agaatatgat gtcacttcat gaagagcttg aagcatttgg 1680ggaaaaaatg ccagaaatca aagcttgtag aacaaagagt aaaggtcgac taaaaacaat 1740tcaagatgaa gacactgatg aagagagctt tgaacaaaat atcgagtaat cacttaaaca 1800tacacggctt attgaaaaat aagtccgaaa aattcacaaa atctcataat atgtgctcac 1860gagatgtctg tttataaaaa tataattact aactatgtac agaggtaaat aaataagtgc 1920atcaat 1926<210>12<211>1949<212>DNA<213>日本血吸虫(Schistosoma japonicum)<400>12cttgagtcac tgtacttgtt taggcgataa ttcttatctg atcaagaaca acttagccta 60tcgacgtgta accttgtaca aaaagtgagt tttaaacata cttggttaag ttttccatct120gatttagttt caggcatcat acatttgcta agatcagaat tttattacaa ttaatcaatg180ataaacgaat atatatctct atagatagtc tgcaaaacgt tcattaaata gtggtccatt240tgagtattta aaattgcttt ttagactgtt tcttatattt tatttcggaa gcgtgcgtaa300aattcaaggc tcagttagat ggaatacttt aaccgaacac ctatcaaaaa cgatattttg360agcattattg gacatctcag ttcgaggaag aaaaacgtcc gtactccatt caacagaaac420catttagcca aaagttcctg tgaaatgcac cctgtttcat tacagtatga cgaacctatt480catgagaaat cacgtagttc taattttgag gaagaaaaat ctagcataaa aaataacgtc540ccgctttgtc cagaaaatat cagcaaaccc tttggtgaaa agtcgccagt gaacgttgtg600atagaatccg attatggttc tgattcatgt atcttagcac ggtctaacag taagagaaat660gacgattatg acaatggact tttaaacttt ctcgaaatta agacaattcc atcaacggct720aaatgtaacc ctgatgtcaa aactaatgct agtttcattc atgacagagt ctttgaggag780ttgctagaat cctccagagt taacataaat atgttagcct ctaatactga cctgggtatt840aaatcgcaca ctcaaacatc ttcaacacct ggtcgtaatt ttatcgttaa tatcaagcca900catagtagtg atgatgatga tgaggccttt gaaaactttc taaaatttca aacagccaaa960ataaataaac ctacaagtaa tcgggatagt gattggttgt cttgggattc cactgacgat 1020gatgagtcaa gttatgattc tccataccaa gctttttact acagagtcaa caataaattg 1080cagttacagt ccgatatcac aagactacct ctaaaattat cagattcaga attatcttat 1140tcagataaag aaaatcttgt tccaaccgag catagtccat catgtactgt tcaagtttcg 1200
acgtatctaa atgcttcgag ctgtcagaaa agtttatgta aaacagggaa acgcaaatca 1260ctatgtgtat ctacggagga attcgtttat tccctttatc cggttagtaa tagtgataac 1320agtaaatatg catcacataa gaaacttcgt catcctgatg cattaaaatt tgttcaaaag 1380tttaaatcaa atcgatctga actagctgac aaattatacc gcatttataa tgagaaaata 1440ttttctaacc aactaccatc aaaattagag attatatgga gtcgtcggct gttgaaaact 1500gctggtcttt gcaagtatat gacaagtaaa accaccctct cagatggaac atctaatcag 1560gttcgtattg cacagatttt gctatcagaa aaggtttgca ctacagctga gcgtgttcgt 1620gatactttac tgcatgaaat ttgtcatgtt gctgtctggc ttattgatgg tttaaacgat 1680ggtcacgggc gtcactggaa atcttgggct aatcgcgctc atcgaatttg gcctaaacta 1740cctttaatat ctgtttgtca tgcttatact attgatactc gattcacata tcgatgtaca 1800ggttgtggtg tatgcatcaa taggcacagt aaatctttgg atattgcaaa aaagtggtgg 1860gaaagaaatt atttcgcata aggagcaatc gaatcacata tttttttact ttccaactaa 1920atcgatatcc aaatcaataa aatattttt 1949<210>13<211>1500<212>DNA<213>日本血吸虫(Schistosoma japonicum)<400>13tgtgtgtttt gggatcacag ttgaacgcgc ttcaatatca acattctgga caaagccgaa 60aatacttctt gcttcgtgac agctggtcgg aataacaaat cttcagatag ttaccttatc120tctaatatat cagacaattt acgtaagcct cacacattca gatggacagc cggcgttacg180ctggcttcga aaccaagcca cacagattcc attcgtaagg aggtcttatt cattcttaat240aagcttactg ctgagaattt cgaacaacaa ggtgaaaaac ttcttagttt agatatttct300tcaacttctc ttcatgtcat tgctcaatta atttatcaaa aagccatcaa tgaacccaag360tttacttcct tgtacgccca attgtgtcta aaattatgca atagcgtccc taattttgag420gtagagggtt ctcaatcgac ttttcagatt ttcttaatca agtgttgcga agatgagttc480aaggctagag acgttgcttg tgatgatcaa gtctcacgtc agaagtgttt ggggaatcta540cgtttcatcg ctgagcttgg gatatgtggt gttatgtccg aaagggtttt gcacaattgt600gttcaagaac ttctcacaag gaaaggtcaa cattatccaa tcaatgattc ggaggttctc660atgcatgcgc aggaaaaagt taaaacggcc gattcattta aagagcgctc tttatctaat720gagttggaat gtctctgtca gttcctgact tctgttggga agcacatgga cacaccaaaa780gcaaagaact taatggacca atattttcaa cgattgcgtc gtatactgtc acgcgcttac840gaatcgaatg gatcgtcatc catcaagcat gaaaatgtat ctataaaagg gaaagttcca900ccaaagacaa taaaatcgaa tgataactgc actttatcgt ctagagtgcg atttatgatt960gaagatttga tagacctaag agaaaataat tggatcccgc gtcgtgctgg tcaacgtacg 1020gaaataaaca aaccacgttt ccttcgagat attcgcttgg agatattaaa ggaatctgga 1080atcctcgttg caccatctcc aagtgaacgc tctgttacac agccagatgt ggctggtagc 1140gctttcatgg ctcattcctc agcctcaagc aactcttcag ttaatggtga atttcctttt 1200gtcagtggtt tgtgttcagg ttcagtgatg aataatgtgg atgaaacaaa aagttggatg 1260gagttggcgc gaatgggtga agaactttgt cgtcaaagcc cccgtgattt cagtttgtta 1320gataacagaa atcgtgttgc agaaagcccc actagtcaat ttaatggccg ttcacagtct 1380aatttagcta cgtctgatcg gcgctttttg tctagcaaaa gcggtttggc ttattcaaac 1440aatattcttc gtcaaaactc aaaatcaaat aattctgata gctggggtca aaaatcagaa 1500
<210>14<211>1059<212>DNA<213>日本血吸虫(Schistosoma japonicum)<400>14taatgcataa cttacacgac tcactcgaac gccaatttgg attttacaag tgaatgatcc 60taaatgattt tactgggctt ttgatgatag ttgatttgtt cactttccta ccttgtagac120ttcacctctg gtcccaagat cacttcttca gttactgacg gaatacatac gaataacacc180aattcagcct agcttacagt aatgcagaag gcactttcgg atattccgac ttggacttcc240atcgctaaag gaacaagcaa tttggcgtcg gatttgggat ctcgtatcag cttgtggcgt300ggtgatataa cacatctccg aattgatgct attgcgaatg ctgcaaatcg tcagctgagg360ggtggtggag gagtagatgg tgcgatacat agagcagctg gtccagaatt acttgtagcc420tgtcagaagc ttggtggctg tccaaccggt gatgcgaaac ttactccagg tttcaatttg480ccttccaaat atgtgattca ttgtgtcgga ccaattggac agaatgatgc agctttggga540tccacatacc aaaaagcatt ggaattatgc tccgaacaca atattcagtc cattgcattt600ccttgtatat ctaccggtgt ttatggtttt cctaatgaag ctgctgccaa ggttgcaatc660catacagtgc tctcctatat gaagtctcat cctgagattc aacgtgtcat cttttgtata720ttcatggaca ttgactacaa gatttacgag aaactaatcc cggaaatgct gagtttatac780acatgatttt atctgtttac cctttctaaa ggcattcgtt tttcgaccac tacatgttta840gtcagttttt gtttactttc catttcttcg atcatgtact gaagcaaata aagtattttt900acatctcata ttatttccag ttatttgtta cctactgttg attactatat aatcagtagt960gtaatttaca ttatctttgc ttcctttctt tgtataacgg tgttcgaatg ttttgtgttt 1020tcaattttca ttgatacagt tatataggat cctgtttcg 1059<210>15<211>2036<212>DNA<213>日本血吸虫(Schistosoma japonicum)<400>15cgcaatccaa aggactgaac atgaaggccc atgtcctacc tgaatttggt agtctttctc 60ttaacgaaaa ccctgctgga tggggcccaa cagacatacc aatggagttc aaaaacatgc120ccttccaacc attttcgaag gatactcgtc taggacgggt tgcagattgg tcgggaaata180tatttcagga tatgaaaact aaaggtcgct atatttctcc gtttgtcggt tcacagtacg240cctactacca tgacgaagat gacaacaatt ttcagctggt ttcatcagtt cgtgagacga300aaactcctgc tattcgtcaa agatataggt tccctatgcg cggtcgtcgt ggtgtgggtg360gtccacttac aggatactca tgggttggag gccggtcagg atatcaaatg atgggaaacc420agggtggcat aataaaccgt aagctacgga atgcctctga tagggaacga atgatgctac480aaaatagacg atggcaacac tggggacctg gtggcctcgg tcagcgacgt ggtggagccc540aaggttggag atctaatact ggtgcttggg gttctaacag tgatcgtaga caggctcaca600gggcagtgcg tgatgcatct attcaaatta aagataattg gacaatgttg gaagagttgg660atttcactag gctttcaaag ttagctctac ctaacgtgaa ggaacctgat aacaaggtcg720attgtggtga aatggagtac tatgataaat catatgatcg cgttagcacg agaaacgaac780gtcctcttgt gagagtaaat cgagttctcc acacagttac aacctccaga gacccggtta840ttcaccggtt agctagtgac tctgttggta aagtatattg tacagacaca attgctgcca900
tgataatgtg ttgcactcgt tcggtgtatc catgggacct aattgtacga cgtatacaag960accgtttgtt ctttgataag cgggaagatg ctgattctga ctttgtcagt gtatgtgaaa 1020ctgcagcaga accacctaat gaagaacctg gtcatattaa ttcaccacaa cgtttagcac 1080tagaagctac ttttataaat acgaatttat cccaacaaat gttattaatg ggcggtaaga 1140catattcttt ccctgaagaa aatcctttca aagatgatga agatgaagat tctgataatc 1200caaatccatc tcataataca aaccgcgaag gtggtaaaga agagttgggt tccgtagggt 1260atcgatatcg agtatttgat ttaggaaatg atattcaaat ggttattcgt tgtgaagtga 1320atggtgtgct tcaaccgaca ggtgatgacg cctctagcag tccacaattt gtttgtatac 1380gcgcgttaaa tgaatttgat tcccgttatt gcggcggtgt cgattggcgt actaaacttg 1440atactcaacg tggagctgtt ttggctgctg agataaaaaa taatgctttt aaactagctg 1500gatggactgt atgtagtata ctatctggtg ctgatcaact gaaattagga tttgtttctc 1560gtgtcaatcc tcgtgattcg tcgcgtcatg tcatattggg aacacaacag ttcaagccaa 1620ccgagtttgc taatcagtta aatttaaata tggataatgc ttggggtatt ctacgatgtg 1680tcgttgattt cttcatgaaa atgcccgaag gaaagtactt aatgctaaaa gatcctaata 1740agcctgtact tcgtatctat tctttaccac aagatgcttc tgattcagat gaagatgaag 1800aaaatataag tgaggaggag gtagctgttg ttgaacaaca aaatgctgaa ccgagtaaga 1860cggaagtgaa taatgtgctg gagcacggtc aagataattc aaagattcag tctcggaaag 1920ttgaagctat ttatcagtcg aaaacaatcg agaataataa accataactt tcattttatc 1980ccaggaaaac ccttcgtata tttactgata cttaattaca caaagtcctt attcgg 2036<210>16<211>870<212>DNA<213>日本血吸虫(Schistosoma japonicum)<400>16gcatgggagg aatatgatat cgctatcaaa aaaggtggct atagttacag gagcaagttc 60aggaattgga agggcaactg ctatcttgtt tgcaaaacta ggagcatgtg ttgctttggt120tgctcgagat aaatcgcgat tgcaagaaac tcgacaagca tgtattcaag tatctcaccc180cgatgtttac gaaaagcata aggagccatt tttgtgcatt gaagctgatc taggtgattc240acatgaagtt gaaaaggctt atcgactggc tttaaatcac ttccatcaac ttgatatatt300ggtaaataat gcaggattta tgatacaaga cactgtagag acctttaata tagatgaata360tgaacgcttg atgaaattga acctaactgc agcaattact ttaacaaatc ttgctgttcc420tgatcttacc actaccaaag gttctatcgt aaatgtgtcc agtgtttgtg gcgaacgttc480attcccggga gtaatgtctt actgtattag caaagcagca ttggatcaat tcactaagtg540cactgcttta gatttagctt cgaaaggaat ccgagtaaac agtgtcaacc cagcagttat600tgttactgaa ctgcataagc gagctggaat gtcagaagac gattataaaa agtttctttt660aaaatctaaa gaatcacatg cactcggtcg aactggaact gtggaagaag tggctcaagc720aattgctttt ctttcaagtt ctgcatcttc cttcactacc ggtaatttat taatggttga780tggtggtcga tctatcatgt gcccacggta gagttttttt ttctacatat tataagttat840ttcttaaata aagtggatca ctggaaatgt 870<210>17<211>2156<212>DNA
<213>日本血吸虫(Schistosoma japonicum)<400>17cataaatttg cgttataatt taagcggtcg actacctgat ttttatctat tggtatacat 60acacagtggc gtcatgatat cgtgtttact gtttctgttt tatgttttct ctttggtgtc120aagtgaagat aagaaagttg attacggaac ggttataggc attgatctag gcacaacata180ttcctgcgtt ggtgtatttc agggaggtcg cgttgaaatt attgcaaatg accagggtaa240cagaattacc ccttcatatg ttgcattcac tacggaaggt gaacggctga ttggagatgc300tgccaaaaac cagctaacat caaaccctga aaatacagtt tttgatgtca aaagacttat360cggaaggacg tttgacgaat cgactgttca ggaggatata aagcactttc cattttctgt420cgttaacaaa aagaacaaac catatgttga agttcatgtt ggatcagaag ttaaatcgtt480tgcaccagag gaaatctctg caatggtttt gggaaaaatg aagagcagtg ctgaagcata540tcttggtaag aaggtaacgc atgcagtagt cactgtccca gcttatttca acgatgcgca600gcgtcaggca acaaaagatg ctgggacaat agctggttta gatgtacttc gaatcattaa660cgaaccaacc gctgctgcta ttgcttatgg cctagataaa aaggatggtg agaagaatat720tctggttttt gatcttggtg gtggtacttt cgacgtttcg ttactcacta ttgagaatgg780agtatttgag gtagtcgcta caaatggtga cacacatctt ggaggtgaag atttcgacca840gcgtgtcatt gattatttcg cgaagcaaat tctcactaag aaaggtaaag atatcaggag900tgataaacgt gcagtacaga aattaagaag agaagttgag aaagcaaaga gaatgttaag960taccactcaa acggctagag tagagattga atcgctcatt gatggtgagg acttttccga 1020aacacttaca cgggcactat ttgagaagct gaacatggat ctgtttttaa aaactctgga 1080gccagttaag aaagtgcttg aggatgcaaa catgaaaaaa gaagacattg atgaaattat 1140cttggttggt gggtccactc gcatccccaa ggttcagcaa ctcctacaaa gtttctttga 1200aaaggaacca aacagaggcg ttaatccaga tgaagccgtt gcttatggtg cggcagtcca 1260gggtggcatt atcggtggtg tggaaaatac tggtggtgtc gtccttttgg atgtatgccc 1320gctaactttg ggtattgaaa cagttggtgg cgtcatgacc aaacttatcc caagaaatac 1380agttatccca acaaagaagt cacagatatt ttctacagcc gctgacaacc aaccgactgt 1440caccatacag gttttcgagg gtgagcgggc catgacgaaa tttaatcatt ttcttggcaa 1500gtttgatctc acaggtattc ccccagcatt gcgtggcgtc ccacaaattg aagtgacatt 1560cgaaatcgat gtaaatggta ttctacgagt atcagcggag gataaaggaa cgggtagtaa 1620aaacaaaata gaaatcaata aggatcaaca tcgtttagac gcagaggaaa tcgagaagat 1680gatcaacgat gctgagcagt ttgcagattc tgataagaaa ctaaaggaac gtgttaatgc 1740cagaaacgag ttcgaaagtg ctgcttacac cttgaaagcc caaatcgcag acaaagctca 1800gcttgcagaa aaacttaact ccgctgacaa aaagaagatc gagaaggcta ttgaggaagc 1860tatttcatac ttagacagca atcctcaagc tgaagtcgaa gaattaaaac agaagaagaa 1920ggaattcgac gaagttgtcc aaccgattat ttcatcatta taccagcaat caggagcacc 1980gcctccaaca caacctgaac aggaaagaga tgaactgtag aggtttgact ttgtcgtttc 2040ccgtgtatat ttccctgata ttgttttcat tcccattttg tttgataaga tatatagtat 2100gtgcttgttt tttaactgtg acagttgata tcaatacaat actggatcct gttcat 2156<210>18<211>1851<212>DNA<213>日本血吸虫(Schistosoma japonicum)<400>18
acattcgtaa tcgagattcc tatgacgttt ttattcttca tgccttttcg aacaacacgt 60ctcgcatcat tttattcgca ggtacttctt caacttgcga taattttgac cggaaactac120aatttcttca atttgttaac tatcgcttta tgttactctc tactgaagga tgatgatttt180aattctcggc gcagacgaaa atggacggta tctggatcat tgtctttcgt aatttcatgg240gttcttatca tttcaatatt tgtcatcagt gcttatctgt tcgaattttc catttcgaat300aactccatta ctagttctgt aggtttcacc aagaaggagt tcacttggtt cctaaacaca360tctgttaaat acagtattta ttttggagta gcattcttct ttgtagaagt tctacacagc420atagttgttg cactgtatgc acgtcgattt tggcgtaagc tctatgaatt agtgggtgtt480attgtcatca gctttattgg tttagctgta ctgatgagca gtttggtacc atttgcatca540ctgaatccaa ctatacaatt accgtctcaa aatcttcagg tttataaaca tttgcagcct600tattatatca caaatagtta tggtctattc cgccggatga ctggagttgg tggtcgaccg660gaacttatct tggaagctgc atcaaatctg aatggtccgt ggtctgagta cgccttcaac720ttcaaacctg gtcgtgtaga tgggagacct ccggttgtca tcccacatca gcctcgttta780gattggcaaa tgtggtttgc tgcgctcagt aactatcgta atcatccttg gtttatgaat840ttaatttatc gattactgaa ccaacaacct gaagttttac aactgctcga tccatcgggt900ctgccgaata atccgaagta tattcgagct catctgtata cataccattt caccgattct960gctaacagca aggattggtg gaaacgtaca ttcaaatctg aatacctgcc accagtctca 1020ttgtcttctg aaatattgcg tagctctatt caagaaaatg ggttggtagg aaaacgtcat 1080cctcgtccac atgatccaac aattttgtca ctatcgttat ctcgtctcag aagttttgtt 1140ggacaaccaa aagatttatc tcctttggtt atggtgtgct tagtgttagc aataactaag 1200tttgcagtta atcaagctga ccaatcgact agaacaactt accatcacaa tagagcttga 1260tgtgtttttc atagttcaaa ttattcaagg aataattcat cgtgtgttga ttatgatgaa 1320gtatttgttt gtatttatgt attatttctt tgaatgcgta tagacgttca tattcttccc 1380attccaatgt ttgttctact ctcctatatc catttagttt tcatattatg tatactcctg 1440acaaaattca ataattcaac cggtcgtatt tgaaataaac tataaacttt tcattcatct 1500attcttaaat tcaataaaag cgtaatctgt ataattaaat tattccttat accgtgtggt 1560atttgaatct ttttgttaac tctctgttca tatatttatg tgtgtgttga attttcactg 1620tgacataaat gtattcttcg gtcatcattt aattcatagt tctggtcatc tgtttcaacc 1680tctttttttt tgtttggtcg taatacatct gaatagttat tttcactgtt catcttgtga 1740ctctgagtta ttacctaata attgtttcat cgtaaataga cacaaaatta attactgtta 1800ctcggattta cttagtatca tgattataaa tatctataga tttattcttt c1851<210>19<211>1187<212>DNA<213>日本血吸虫(Schistosoma japonicum)<400>19tgtcgataaa gcggtcgtta tgtcaggtac acatcaaaac catgcagtta gtattcctgt 60gaaccgtgaa caatgttcat ttccgaggca tagacacgaa ctgctacaga atgtggggaa120agccagtaaa agcggtcagt tgggttcagt agtgccatac acggaagact ggcccagttc180agtgagtaac tggatagagt cttcgttgaa aagctgggac aaagaaatgg aacgtttaag240gcgaggaatg tttgctctgt tgccaatgga tcgcttcatg gtcggaatcc cacatgatcc300gttgggaatg atgcatgaaa tggatcgtca cattgaggaa cttcaaaatg ggatgggttt360aagtgctgtg gcgccgcttg gatcaacgag tgactacttg aaggatgcct acgaagtcgg420
cgatgatggc aaggtgcatt tcaaggtgag atttgacgca cagggttttg ctccagaaga480catcaatgtg acgtcgagtg acaaccgtgt gacagtgcac gcgaagaagg agacgacaac540aggtggtaag aagtgtagtc atgagttctg tcgtatgatc cagttgccga agagtattga600gaataaccag ttgaaatgcc gtctgactga tgatggtgtt ttgatgttgg aggctccagt660gaaagttggt gagaataagt cgctgacaat gaacgagtct ggtcaggtgg gtattcaacc720gaaatcagcg agtcagatac aagcagttcc tgcatcgcaa gctctcactg tgaaaggttg780tcaaggtctg acggtgttgg atgatggtgc aggtggcaag aaactgcacg ttgaggttca840attggaccca gtgtaccgac cagaagattt gtgtgtgaat attgattcga atcgtgttgt900ggttagcgga cgtcattaca agcaaaagac tgatgcaaga agaaagtcga gttcattcgc960agaattcagt cagtcgtatt cgattcctga gactgtggat ccgttaactg tgtctgctca 1020ggtagttgac aatatgttgg ttgtagaggc accgatgatg aagcaacacg aaatcgctca 1080ttagacgaaa atatgattga ctgaaatcac aataattatg gtaatgatga tattcacttg 1140tgttatttaa caaataaata catttgctta ctcgctattt gcttttc 1187<210>20<211>822<212>DNA<213>日本血吸虫(Schistosoma japonicum)<400>20tttgtccgac aacaccaagg cttccagggt tagaaggaga aagggggaga gatacaaacg 60ggcaacgtaa tgacagaagt atgacgtcaa gttcaggagg ttccattaaa gacaaaagaa120gtgatcattc atcaacaaaa gaaaagaatg atattagcac agaaagcatg tttggatcat180ccacatctag atatcgatac aggaaaacaa aagattttaa agccaaaggc aaactccgat240cacgcggtat tggaaaatat ggtaaaatgt catctgcatc gacagatttt gtagttgaag300ggaagatcgg taaatatggt cataggaaac ctcttaaatc gaaattcaaa actcgtggga360aggagaagaa atattcaaga aagattagtg acaatcggtt cgatattaaa ggtgatttgt420atgaacgacg tcaacatgag ggtattggtg attatcaagc aaacggtagt tatattaatg480taaaactaaa aactcatcaa caaaatggta gtaaaaataa aacgcgccaa aattctggat540caaaaagcca tacgaattca tctactcaac gtgaaaacag ccgttgggat gaacaacgaa600gaaatacatt caattaacat gaatataata ttttacaaca tggttaacat taatttaatt660tctccagaat ttttttaatt ctaatcatcc cactaatatc caccactgaa acatattatt720tatcgattta ttttcatctt catacacgct gttaattaat gataaattca actacttggt780gtgagttatt tttgttaaat ctaaatcaag tttaataatc ta 822<210>21<211>2048<212>DNA<213>日本血吸虫(Schistosoma japonicum)<400>21gcgaatcgtt ggggtcgtaa tgggtcagat ttatggagct caaatattat cgtatgcctg 60acaccaaagc cccagctttt acagaggctg aagaagaaag ggacataaca tccgaacgcc120ctgaggttgt ggttttcttt ataccaaatg cagcccattt aattccctct gaagaacagt180gggcccggac taaagaatac tataactcca ttttacagaa gcagctgact acagtgaaag240tagaagagga tggggtgact gagcagcaag acggtgagat gagtgttgca gatgttagtg300
ttgatgaacc agaggaatca gtcgcacgct ctgacattga accgactcac tattccaagc360tcgatgtaaa tactttaaag gttaatgact tgcgcaatga gttggctgcc agaaagttag420acacaaaggg tctcaaagtt aatttagtgg cccgtttaca aaacgccttg gatgaagaga480aaaaggcgga tgctccagaa gacattaagg atgatgagga gtccaaaggt aagcagtgat540atatcttgtg cctcgctttt tagtaattta gagttactag gaccacaaga acggctgtgg600atgtttttat tgtgataatt aaccaaacaa atggttatga aactcatatc gccttctttc660tgcttgtatt cagccgaaca gactccgaat aagatcactt cgccgtctgc gacgcagtca720aaggatgatt caaaggacct atccgaaaaa gatcgccggc gtctggaacg cctctaccgc780cttggtgata aaccagccat cgttatccat ccaaacaagt cagctaaagg gggtcgtttc840gactgtcacc gtgtttcttt gttctctctc ctcgactatc gtactgaaga ccaaaaagag900cacaattttg aagtgtccct gttcgctgaa cagtttcatg aaatgctcca gcgagatgca960gccttcacaa tattcaaggc tattcacgat gcacctgaga aagaaattaa acctgaacaa 1020gatggaaaac tcgagaacgg tttcgattcc gatgagcctg agtctaagcg acgcagaaac 1080gatagtcaaa acggttataa tgatgagcag gacgacgtga agatccgtcg cctcagacgt 1140actgtgaacc cagccttgtt atttgcgttt acttacttcg acataagtcg ggctggatac 1200attcgggaac atgatttgtg tgaaatactt catcttctcg gacttcggta ttctcgtacc 1260caaatgcgta aacttgtagc cagagtagct actcgtcgtg atcatgtgtc ctaccacaat 1320ttgacagaca gcgacattgt tgaaggtgat gataaagatg tcgtatccat gaacgtggct 1380gtggaagagg atgttgaagt gacaagaatg cttgcacttg gcaaccgagc cctgttcgat 1440atgacagttc cgcaaactcc agcgaggatt gttattggtg gagggaatac ggaacagatg 1500ttaaagcgtg tggagttagc tgaggcagtc aaacataaga tggaatttca gtttttccag 1560ataaaggatg agctagaaaa aacacgagtc aaagtcaact cttttaaaga tactgaggat 1620gaactaagag cagagaatcg ggatttagcg tcacgactaa aatccgaaca aaagcaattg 1680cacgagtaca aaagtcaggc aaatacatac aaccacttgt tgcgcttagc cagagatcag 1740atggataaag ttcttttcga catcaataag cagtttgaca agggaaaagg caaagcgcga 1800agccgaaaaa gctgccgccg atgcggagaa agccaaggct gaggaggagg ccaataaact 1860taaggaagaa aaggacgaca aggtcgagga tagtgaaaag tctgaggaaa aggaatggga 1920agttgttgac gctgtctaat ccgagacatt gcatttactg tggagcgttc agtttctcaa 1980ctcatttgta cgtcatttta actaaactca acttttccat acacaaataa aaaaaaaaaa 2040aaaaaaa 2047<210>22<211>831<212>DNA<213>日本血吸虫(Schistosoma japonicum)<400>22gttccgtcaa aaacgaaaat ggcgaatcgt tggggtcgta atgcacctta tggtggtgga 60gcaggcggtg gcggtagtgg tggttacatg ggttctgggg ttgttggtgt aggtttttac120atgggtggac ctacgcctaa taaccgcaga ccgtacaacg ctgccccggt tgctaaaccc180ttttacggtg gatatcgaga agataattac aacaggagat cagatcgacg agatgatttt240cgtggtggta aacgcggcca gccgaaagga cgaagcccag agaaacggta tcatgatgca300ccatctagtc agcgaaaaga tcacagacca tttggaaagc gacagtcatc gttttcacgc360tatgaggtga agatccccaa ggggctattc aatattactt catcatcttt acctgacctg420cgacatcgat accctcgcct caacatatcg ggagacttct atgagtgtac atcatcatgg480
gtagaatcat ttcccatgca tgaaccgttt gaactaggaa gtggcactga ttaccacgtt540ttccaccggg acgttgatcc acctgtacct ccagactccg ctctcctgaa cccacctgat600gcagattact cctattcagc cagagttatg ctaatggctt gcccgcaact cggtgagtta660tacaaaaata cttgtcgtat ggcggacgat gccagcggag ctgataaagt tctgcctgat720aagagtattc actttctggt tggtggtcgt gcaaagagtg agactatggc tcttggtgga780ccttggtcac caagtcttga cggacctgat ccacagggag acccaagaac t 831<210>23<211>2497<212>DNA<213>日本血吸虫(Schistosoma japonicum)<400>23ccacatgata cgtcctttga gttcaagtga atacactcaa atggctggca gagcaggacg 60tcgaggttta gatgctaaag gttatgtcat tattttagtt agtagcattg gtaaatcact120taaatcaagc gtgactggtc ttccgacaga aggtgtgttg agaagtgtca tacttggaac180gcaaactcgt ttagtttcac aatttaaagt cacttattcg atgattttaa atttacatcg240atcttcctcc ttaactcttc gggatgtcat gaagcgtagt tttatggaag cagcaagcca300tagatgggag actgaacaac gacaacacct atcatttttg aataagaaat tagatgaaac360tactacactt atttcatcga atgattccaa tcaaacatct gttaattcag tttcagcatt420cactgagaat aatgatgatt tatctgaatc attgtttgtt tatcttgatg tacgtgttaa480atgtccatac aatggaattg aatgctgtga ttcaataagt gcgtactacc agctatgtta540tagatatcgt caattaaccc aatcacttat tcgtacactt agtacaaaat atgaaagata600tttacgaaac atattttgtc ctggtcgtgt tatattactt catgtaacta taaaacaatc660tgtctggtta gttcctggtg ttatagtaaa ttattactgg gagacgaaaa aaaatggtaa720agatcaatta ctgtttcgtg ccattctatg gaagctgcct actgactcgc ctacattttt780caccaaaaag caagaagggg aatcagaaac ttcagcagta gtaggtagta ataataaaat840atcaacggcg ccggtgggtg aagataatca ttcttggtac aactgcgaat atgatgaaat900tgaagagaaa attctacgca atatcaaagg tagttgtaat acacagtggg aagaaacacc960agtaccacct catttggttc cagtctttat gccatcttca ttagaagatg gttatgcacg 1020tgtgacactt caatcgattc taccagagga ttcacttttt agaatttgtg atcgaattgt 1080tacatttgaa aataaatccc taaaattgaa tatgaatatt tcagattgtt tattgcatag 1140tattaagcaa catcagaata ctgtcaaccg tagggaaacc agtgtcaatc tacctgatga 1200taataatgaa cactacttcc ctattttaaa tgatgttaat ttggcgcttt ttaattctgt 1260gaattcacca caacataact cgtcattttg tgttatttac acgacattaa gtgataatgt 1320accaaagcaa gtagatatta cgaaggaact aaaggctgat aaaattgatg aagaggatgt 1380tcgcttattt gaagaatatt ctacgattaa tgatcagtta acttatcctc ttagtataag 1440cgacacaaca actttaaatt cgaatctgct taaatgcccg aatttattac aacatttaaa 1500tcgtgttcat caaacaatac gtcgtaaatt aactatcaag aaaatagaaa ctaatctagc 1560tgattatcaa ttacaacttt ccaaagaata tacaggacgt ttaaacgttt tgaaagaact 1620tggattcatc gattccgcta cacaaagtta ttgtcttagt tctaaaggtt tctttgcatg 1680tgaattaata aaaaaggaag ttgtattgac tcagctttta cttgatggtt ttattgataa 1740tctattggcg cctgatattg cagctgttct ctcagttttt gccaatgaac tacgaccata 1800tgatttttcg ttggaaaaaa aatcaagctc ttatcttaaa gaattagtta attcaataga 1860ccgtatcaat tccaatacaa attatgtatc gaataatgac ttagatttaa ttcctcgaca 1920
tttacttcca gtattcaaaa gaatattaaa ctgtgcttat gaactggaaa cactacagag 1980atttcacaat ttgattgatc catatttgga aagtcgtttt gatctacgtt tagttccttt 2040agtttataaa tgggctaaag gatactcatt ctctgcaact atagcaaaat gtgatctacc 2100tgaaggttta ctgattaaaa gtctattaca acttgatgaa ttgattcgac atattgtcgg 2160tgcatgtcgt cagtttggca agcatatatt aagcttaaag atgtgtgaag ctagaaatct 2220aatatatcgt gatattgttt cttcaccaag tttatatatt ttaaaaggta taaaatcagt 2280tacagatgat tgaaaaagag attgcatcat aattttttta acactcaatt aagtatcatt 2340aattttttaa ggatattttg tgtaatatca tgttgaaatc tcatataacg ctttctagtt 2400catcagatta cttactttaa gtcatctttt tatactttta aaacctacaa ttattttatt 2460aaaatacatt tgattattaa aaaaaaaaaa aaaaaaa2497<210>24<211>1841<212>DNA<213>日本血吸虫(Schistosoma japonicum)<400>24ttcgtaatca gcaatgttag cataacaatg aagttaccaa cgtttaatat atttctgctc 60tcagtcacac tcgtttatgc aaagttttgg cccgcggtat acagtaattg cgttacgaag120gcctagtgcc tttggtgcat ggaaagactt gattctctac tttgtcgatt agaaaaagca180acgattatcc ttgaagcagc tgctgttaac agtatgtatc gaacatcacc aatcaagacg240tcctcagaaa gtaggaatcc tttatcagtc aaagaatttc atgatattat tactggtcct300ttgtcggagt atgtattata tagctccaag attggagatg ttgttaaaac tcatgcttct360gtggttgaaa ggtgtttttt aatggaagaa gaaatactca aattagcctc tgaatgctcc420aaaccttccg actctgaact tacacttatt atgacactac tgggaaagga aatagaagaa480gttgttaaac ttaaggataa caatcgaagc aatagattct ttaaccatct ttctgctgta540gctgagtccg tagcagcttt gggctggtta agtgtttcac ctgctccagc accatacatc600aaaactatga aggaagctgc tgaattcttc acaaatcgtg ttattaaaga atacgaagaa660aaagacacag tccatcttgc ttggaaaaaa gctttaatgt cagttttgac tgcattagag720ctgtatgtta agaaacatca tactactggg ttggtttgga atgctcgtgg caaacctgca780acggcatcca aactgtctaa gtcctcacct actcgtgaag ttaattccaa ctgttctgta840gctctaccac caccacctgc acttacacct gcccacttag ctgcaatatc attgcaagga900aaaaatcaat caccacaatc tgctctattt tctgatatta atcgtggaga agctgtgaca960tctaatctac gcaaagtgac tgatgatatg aagacacata aaaatcctaa acttcgtgaa 1020ggacctatta tgcatacatc acgacatcaa gtgaattcat cactgaataa cacaattatc 1080cgatcgccag ctgagaaaaa gggatgcttg gaactaaagg gtaataaatg ggttgttgaa 1140tattttaatt ctgtggaaaa tttagaggtt attataacag aacctaaaca gacagttttc 1200atttataaat gtaatgaatg cactattcgg attaaaggga aagctaattc aatcatgttg 1260gataattgta agaaaactgg tgtggttttc gattatttga tctcatcgat tgatgtagtg 1320aattgtcaaa gtattcaaat tcagtgtctt ggaaagctct caaccgtgaa tattgataaa 1380acagatgggt gtcaagtatt tctaaatgaa gactcaaaat atgctgatgt gatcacagcg 1440aaatcttcgg aagtcaattt acttgtaccg aatgcgactg gtcatatcga ggagtttgct 1500gtcccagaac aatttaaaac aaactttact ggaaacggat tgaaaactgt atgcactgac 1560tccagataaa tgtcatttga ttttttttaa gttgcataac atctaaatgt ctcaataatt 1620ttggaattaa tttttaaatg gctgctttat catgctttat cctataacac tagtacagaa 1680
ttttcgttta atcttaagaa aacactactt ttatcaatga ctgagtctga ttatgtcgct 1740ttttttaact tatgatatta agcacttatt ctatatctgc tctctactgc ttgttcgaaa 1800atatacagct aacggttaaa aaaaaaaaaa aaaaaaaaaa a 1841<210>25<211>224<212>PRT<213>日本血吸虫(Schistosoma japonicum)<400>25MSSLEASQILASALKDVDEILKVSNCESNDICNAYDNPNNQYTLPEKSVQNVQRQSHSIN60VDNSTTCDETGHYRTEKEFFTLMNRLHKCIEKLHLSPDITPLHTPEYYPSNFRVTENGHF120NLVEPTITQPTDCNLINNKQLQLQQQQPQHIVLSNDYTNLNDKLLHQRIIRLQAQIQAQA180NRIAEMKMDKTPSSHLVNRTNRTDQNDTMNQFTIDMDNRQLLNE224<210>26<211>298<212>PRT<213>日本血吸虫(Schistosoma japonicum)<400>26MDAHCQRVLCLDKTAVRCFLIVSACRKEINLCNSIKLDSELSRFFTSLYDVDENAVNSGI60DYRLNLQGKLTRAGDIVDLASKPMFEYVNEDIFKKRPTFTKFISLLDNYNPKVGVTEIVT120QQQQNEENEFINELLKTSIMKMTHTFLVEKQKLSGDINDFGKYLKELWFRRYQRRSPGDS180SAFEHVFVGEHKGLEVLGLHNWIQFYLKEKNNQLNFFGWTKRLCNDQLITVSYVNENKYK240KPMGSFFVGSSPEFDIAVYTVAFALHQKSAFEVNIAGCKVRITCHTLSSSEMGTCYMG 298<210>27<211>243<212>PRT<213>日本血吸虫(Schistosoma japonicum)<400>27MSEMGSGYGSPQIQRRYLIPLAEQFVRISIDSSSVDEFVEYIIQNKSTSCSRSNIKLWRK60DACKALRLANTRTIVAPVPKDSPVTVDGCESSKVDADRRFPRKRSKLSLEPPAEPISPRT120ASCTLSNIRCTVPMDLQKQIKGFLTCHLLSDLGLPVWPTLLVNGPPFCGKTTLIEAACGT180LGLKIITVSVGTVTSSMRSWMDIFNQAKVSSPCILHLDDIESMEKHSSPVGAFRLTCLLK240SHL 243<210>28<211>343<212>PRT<213>日本血吸虫(Schistosoma japonicum)<400>28GIPGTGKTASVQAVLSTMHKLVADSCLESQLPVFQTIYVNGMRVSDPKQIYIQIYEQLTG60LIATTKSACDLLEKEFCSSTNKKLNHREVSEKPVILVIDELDLLCTRRQDILYSLFDGPT120RHNNRRVLIVLAIANTMDLPERLLHPRVASRLGLTRLTFAPYSHEQLSQIVRHRLSSLSN180
ILQPKALELAARKVAAVSGDVRRALDICKRAAEIVSSSEKTNKEIDISHINAALKEMFVT240PKSDAICACSLYEKLFLRAVIAEFQARSTEEARLDRCIRQMSALCRLEGVPCPTTSEVFA300ICASLGAHKLLLTERSRYDIAMLVRLNCTKSDILYCLNSQSNG 343<210>29<211>119<212>PRT<213>日本血吸虫(Schistosoma japonicum)<400>29MFLSVFLICNILLCTTSTFKLVNERSTNEEDHSDSQALKLSKLQSEDYAPRYLVPDIEEF60NISSPFINEQLRNQMDILKQELEEIAKKLNHVPNSCNSNLYNGLLVSICIGLFSIITLV 119<210>30<211>416<212>PRT<213>日本血吸虫(Schistosoma japonicum)<400>30HEWKGAWCDGAPQWREISQKEKENIHLNFTEDGEFWMSFEDFVTCFSRVEVCHLGLESLE60FNQDFHGKRRLEEAIFSGQWQRNVNAGGCINNRTTYWTNPQFLITVEDPDPDDNDNKCSI120LVALMQKETRKKVGADFQPIGFMVYAVPDDQTTLMSRAQLLTKTPIAKSQFINTREVVAQ180FRVPPGRYVIIPSTFDPHIEANFILRVISQIPITEQELDEDNTNRGLPDDIIESLKLEDT240LLDEDKEIEMRFMALRDPKTLAIDATKMGELLNNSTLQDMPSFKGFNKELCRSMVASVDN300NLTGLVELDEFMDLWIQAKGWKHIFLKHDIDQSGYFDAYELREALNDAGFRVSNLLFNAI360AHRYTDPGTDKISFEDFMLCMVRLKTAFETIEAHPKNLEGTSLFMKEDYLRFTVSI416<210>31<211>238<212>PRT<213>日本血吸虫(Schistosoma japonicum)<400>31MAGSVSRGIFVVLEGADRVGKTTQASLLAKALSEITQKKTLSVKFPNRDTPLGHQLDAYL60GGNGDINSHALHLLFTANRWEKQSEIRNAISNGISVVADRYIYSGIAYTAAKLPPTPNWE120WCCEMEKGLLEADLVICLTPENLDELNSRNGYGNERYEKDDFQKRVLENYVRLSKEVELD180NSEWQNNDETGDSVGLWHFIQATNKTVEEVHKCIMVLVKSKLESISGPEIHDCTKKKD 238<210>32<211>117<212>PRT<213>日本血吸虫(Schistosoma japonicum)<400>32MKLSELKRELGQCLDEKQEIATERDIYRNKCDRLNNELNYILSGDGRKIQDIDTLMAESR60MLKDQIELLQMEKSLALNTVTKYKKLLDRKLSKHSSSVSSESCITPAISPKHQEKTI 117<210>33
<211>587<212>PRT<213>日本血吸虫(Schistosoma japonicum)<400>33LEGLRGAEGEVGEAGEQGETGLPGEEGDQGEEGPIGEIGEIGETGTVGDIGIEGNEGPLG60DQGSEGDIGMRGEYGPTGEKGEVGLRGDPGKAATDCPIRPKGEQGRLGPPGLPGPQGPPG120DQGLPGLRGKVGAPGVGVIGAPGEPGDSGHIGLPGEAGEPGDQGPEGPSGLAAVTGEQGP180KGERGIPGDVGDPGVDGVQGPPGYDGEKGTQGPQGTKGTDGVDGTPGPKGFSGDRGPSGN240RGQIGDQGETGVAGIQGVKGVQGIAIAGKLGETGDPGENGVTGEQGDPGPDGELGEDGEM300GEKGEKGQPGLLEGERGPVGDQGERGDIGEVGPSGPVGDIGPKGRDGEKGQPGQTNYSSI360LFARHYQTPFVENLTCPGGTNKLFTGYSYVMGGGVDDLVSMDLGTPSSCLSKFSSLPMTQ420CERDTTCQSSMRHERSYWLATLVPRSEQPIPVNQTADQIARCVVCEAPSHVFAFHSQGET480LQPCPSTWTELWTGVSLILHTSGAHGGGQQLSSPGSCMEHFRYSPVIECNNNVGMCHYWS540DAKVYYLRALNPNITQFEKPVGFVMKAAEGPVLNNVSKCRVCMKIPA 587<210>34<211>485<212>PRT<213>日本血吸虫(Schistosoma japonicum)<400>34MIDPPNTPYGPLPCGAINKILVNPSANGGLLTVFCGGLPRPQFENRYAVSVLQDHEHHVC60YQFGSKVIDFVLIPSEGNMSNETVPGNFMTKPSAPSHSATLLVLTERELVAVDLTQPDWP120AYDSPYLNCMDFSPVTAITHIGQVPSALIQRLHQAAHTTTDDITNCSWPIWGGSQADGNK180KNLAQNAGNDIVALGHSNGWVTLWAIGRGDTTIHLGTLPTSSLFNSADLQNDQKCGSSAL240GEETWPPFRPVGYCSRAQRHTLEGCDPRLAISQLLILLGSPIRSGVSDSVAPDTLTLVVG300GASGQVSLWVAGSEGFLHTPELTSFEPDVSRVYINLIDQSEEDKYIWKDAPCLRPTEGVP360QYCTPTGLTFHPSHLVQLNPPSQITSLALELSWNLLAVGSSHGFALLDLFNRSIIHTHFT420YDSSGPAKIFNTVSTVQNVIMARGKQIKSNIRHSFRRLKNLRTSTTTTERSLTEPAPVDE480QYTPN 485<210>35<211>341<212>PRT<213>日本血吸虫(Schistosoma japonicum)<400>35MKVVDDIVQDMGCGEQCEVTLTSDYEFTDSERSFLKISDECFHKILFNIYLEDIQKLAEF60WQMSESEKFIIADVMKCRGNKLLKAGLLIRAFHCYKKAIRILEGSISVTNHDCTNLAIPD120SESSKPTPRQLLAICLSNAAHCLFQSGTTCDTNLKKSQFDYLSACIKCCKRAVSLEPNYM180KAWFRMAKTHALLGNYEEAITAGEKCLSRSTNENISESKWINVDERTKTYITNKIKTLLS240EWRVSLTNEEETERSIIRKRTIKKYSSFGDWCCDELDEIEKLPISIWSNDLAENMMSLHE300ELEAFGEKMPEIKACRTKSKGRLKTIQDEDTDEESFEQNIE 341<210>36<211>520
<212>PRT<213>日本血吸虫(Schistosoma japonicum)<400>36MEYFNRTPIKNDILSIIGHLSSRKKNVRTPFNRNHLAKSSCEMHPVSLQYDEPIHEKSRS60SNFEEEKSSIKNNVPLCPENISKPFGEKSPVNVVIESDYGSDSCILARSNSKRNDDYDNG120LLNFLEIKTIPSTAKCNPDVKTNASFIHDRVFEELLESSRVNINMLASNTDLGIKSHTQT180SSTPGRNFIVNIKPHSSDDDDEAFENFLKFQTAKINKPTSNRDSDWLSWDSTDDDESSYD240SPYQAFYYRVNNKLQLQSDITRLPLKLSDSELSYSDKENLVPTEHSPSCTVQVSTYLNAS300SCQKSLCKTGKRKSLCVSTEEFVYSLYPVSNSDNSKYASHKKLRHPDALKFVQKFKSNRS360ELADKLYRIYNEKIFSNQLPSKLEIIWSRRLLKTAGLCKYMTSKTTLSDGTSNQVRIAQI420LLSEKVCTTAERVRDTLLHEICHVAVWLIDGLNDGHGRHWKSWANRAHRIWPKLPLISVC480HAYTIDTRFTYRCTGCGVCINRHSKSLDIAKKWWERNYFA520<210>37<211>309<212>PRT<213>日本血吸虫(Schistosoma japonicum)<400>37MSERVLHNCVQELLTRKGQHYPINDSEVLMHAQEKVKTADSFKERSLSNELECLCQFLTS60VGKHMDTPKAKNLMDQYFQRLRRILSRAYESNGSSSIKHENVSIKGKVPPKTIKSNDNCT120LSSRVRFMIEDLIDLRENNWIPRRAGQRTEINKPRFLRDIRLEILKESGILVAPSPSERS180VTQPDVAGSAFMAHSSASSNSSVNGEFPFVSGLCSGSVMNNVDETKSWMELARMGEELCR240QSPRDFSLLDNRNRVAESPTSQFNGRSQSNLATSDRRFLSSKSGLAYSNNILRQNSKSNN300SDSWGQKSE 309<210>38<211>194<212>PRT<213>日本血吸虫(Schistosoma japonicum)<400>38MQKALSDIPTWTSIAKGTSNLASDLGSRISLWRGDITHLRIDAIANAANRQLRGGGGVDG60AIHRAAGPELLVACQKLGGCPTGDAKLTPGFNLPSKYVIHCVGPIGQNDAALGSTYQKAL120ELCSEHNIQSIAFPCISTGVYGFPNEAAAKVAIHTVLSYMKSHPEIQRVIFCIFMDIDYK180IYEKLIPEMLSLYT 194<210>39<211>648<212>PRT<213>日本血吸虫(Schistosoma japonicum)<400>39MKAHVLPEFGSLSLNENPAGWGPTDIPMEFKNMPFQPFSKDTRLGRVADWSGNIFQDMKT60KGRYISPFVGSQYAYYHDEDDNNFQLVSSVRETKTPAIRQRYRFPMRGRRGVGGPLTGYS120WVGGRSGYQMMGNQGGIINRKLRNASDRERMMLQNRRWQHWGPGGLGQRRGGAQGWRSNT180GAWGSNSDRRQAHRAVRDASIQIKDNWTMLEELDFTRLSKLALPNVKEPDNKVDCGEMEY240
YDKSYDRVSTRNERPLVRVNRVLHTVTTSRDPVIHRLASDSVGKVYCTDTIAAMIMCCTR300SVYPWDLIVRRIQDRLFFDKREDADSDFVSVCETAAEPPNEEPGHINSPQRLALEATFIN360TNLSQQMLLMGGKTYSFPEENPFKDDEDEDSDNPNPSHNTNREGGKEELGSVGYRYRVFD420LGNDIQMVIRCEVNGVLQPTGDDASSSPQFVCIRALNEFDSRYCGGVDWRTKLDTQRGAV480LAAEIKNNAFKLAGWTVCSILSGADQLKLGFVSRVNPRDSSRHVILGTQQFKPTEFANQL540NLNMDNAWGILRCVVDFFMKMPEGKYLMLKDPNKPVLRIYSLPQDASDSDEDEENISEEE600VAVVEQQNAEPSKTEVNNVLEHGQDNSKIQSRKVEAIYQSKTIENNKP648<210>40<211>265<212>PRT<213>日本血吸虫(Schistosoma japonicum)<400>40MISLSKKVAIVTGASSGIGRATAILFAKLGACVALVARDKSRLQETRQACIQVSHPDVYE60KHKEPFLCIEADLGDSHEVEKAYRLALNHFHQLDILVNNAGFMIQDTVETFNIDEYERLM120KLNLTAAITLTNLAVPDLTTTKGSIVNVSSVCGERSFPGVMSYCISKAALDQFTKCTALD180LASKGIRVNSVNPAVIVTELHKRAGMSEDDYKKFLLKSKESHALGRTGTVEEVAQAIAFL240SSSASSFTTGNLLMVDGGRSIMCPR 265<210>41<211>648<212>PRT<213>日本血吸虫(Schistosoma japonicum)<400>41MISCLLFLFYVFSLVSSEDKKVDYGTVIGIDLGTTYSCVGVFQGGRVEIIANDQGNRITP60SYVAFTTEGERLIGDAAKNQLTSNPENTVFDVKRLIGRTFDESTVQEDIKHFPFSVVNKK120NKPYVEVHVGSEVKSFAPEEISAMVLGKMKSSAEAYLGKKVTHAVVTVPAYFNDAQRQAT180KDAGTIAGLDVLRIINEPTAAAIAYGLDKKDGEKNILVFDLGGGTFDVSLLTIENGVFEV240VATNGDTHLGGEDFDQRVIDYFAKQILTKKGKDIRSDKRAVQKLRREVEKAKRMLSTTQT300ARVEIESLIDGEDFSETLTRALFEKLNMDLFLKTLEPVKKVLEDANMKKEDIDEIILVGG360STRIPKVQQLLQSFFEKEPNRGVNPDEAVAYGAAVQGGIIGGVENTGGVVLLDVCPLTLG420IETVGGVMTKLIPRNTVIPTKKSQIFSTAADNQPTVTIQVFEGERAMTKFNHFLGKFDLT480GIPPALRGVPQIEVTFEIDVNGILRVSAEDKGTGSKNKIEINKDQHRLDAEEIEKMINDA540EQFADSDKKLKERVNARNEFESAAYTLKAQIADKAQLAEKLNSADKKKIEKAIEEAISYL600DSNPQAEVEELKQKKKEFDEVVQPIISSLYQQSGAPPPTQPEQERDEL648<210>42<211>419<212>PRT<213>日本血吸虫(Schistosoma japonicum)<400>42TFVIEIPMTFLFFMPFRTTRLASFYSQVLLQLAIILTGNYNFFNLLTIALCYSLLKDDDF60NSRRRRKWTVSGSLSFVISWVLIISIFVISAYLFEFSISNNSITSSVGFTKKEFTWFLNT120SVKYSIYFGVAFFFVEVLHSIVVALYARRFWRKLYELVGVIVISFIGLAVLMSSLVPFAS180
LNPTIQLPSQNLQVYKHLQPYYITNSYGLFRRMTGVGGRPELILEAASNLNGPWSEYAFN240FKPGRVDGRPPVVIPHQPRLDWQMWFAALSNYRNHPWFMNLIYRLLNQQPEVLQLLDPSG300LPNNPKYIRAHLYTYHFTDSANSKDWWKRTFKSEYLPPVSLSSEILRSSIQENGLVGKRH360PRPHDPTILSLSLSRLRSFVGQPKDLSPLVMVCLVLAITKFAVNQADQSTRTTYHHNRA 419<210>43<211>354<212>PRT<213>日本血吸虫(Schistosoma japonicum)<400>43MSGTHQNHAVSIPVNREQCSFPRHRHELLQNVGKASKSGQLGSVVPYTEDWPSSVSNWIE60SSLKSWDKEMERLRRGMFALLPMDRFMVGIPHDPLGMMHEMDRHIEELQNGMGLSAVAPL120GSTSDYLKDAYEVGDDGKVHFKVRFDAQGFAPEDINVTSSDNRVTVHAKKETTTGGKKCS180HEFCRMIQLPKSIENNQLKCRLTDDGVLMLEAPVKVGENKSLTMNESGQVGIQPKSASQI240QAVPASQALTVKGCQGLTVLDDGAGGKKLHVEVQLDPVYRPEDLCVNIDSNRVVVSGRHY300KQKTDARRKSSSFAEFSQSYSIPETVDPLTVSAQVVDNMLVVEAPMMKQHEIAH 354<210>44<211>204<212>PRT<213>日本血吸虫(Schistosoma japonicum)<400>44CPTTPRLPGLEGERGRDTNGQRNDRSMTSSSGGSIKDKRSDHSSTKEKNDISTESMFGSS60TSRYRYRKTKDFKAKGKLRSRGIGKYGKMSSASTDFVVEGKIGKYGHRKPLKSKFKTRGK120EKKYSRKISDNRFDIKGDLYERRQHEGIGDYQANGSYINVKLKTHQQNGSKNKTRQNSGS180KSHTNSSTQRENSRWDEQRRNTFN204<210>45<211>403<212>PRT<213>日本血吸虫(Schistosoma japonicum)<400>45MVMKLISPSFCLYSAEQTPNKITSPSATQSKDDSKDLSEKDRRRLERLYRLGDKPAIVIH60PNKSAKGGRFDCHRVSLFSLLDYRTEDQKEHNFEVSLFAEQFHEMLQRDAAFTIFKAIHD120APEKEIKPEQDGKLENGFDSDEPESKRRRNDSQNGYNDEQDDVKIRRLRRTVNPALLFAF180TYFDISRAGYIREHDLCEILHLLGLRYSRTQMRKLVARVATRRDHVSYHNLTDSDIVEGD240DKDVVSMNVAVEEDVEVTRMLALGNRALFDMTVPQTPARIVIGGGNTEQMLKRVELAEAV300KHKMEFQFFQIKDELEKTRVKVNSFKDTEDELRAENRDLASRLKSEQKQLHEYKSQANTY360NHLLRLARDQMDKVLFDINKQFDKGKGKARSRKSCRRCGESQG 403<210>46<211>271
<212>PRT<213>日本血吸虫(Schistosoma japonicum)<400>46MANRWGRNAPYGGGAGGGGSGGYMGSGVVGVGFYMGGPTPNNRRPYNAAPVAKPFYGGYR60EDNYNRRSDRRDDFRGGKRGQPKGRSPEKRYHDAPSSQRKDHRPFGKRQSSFSRYEVKIP120KGLFNITSSSLPDLRHRYPRLNISGDFYECTSSWVESFPMHEPFELGSGTDYHVFHRDVD180PPVPPDSALLNPPDADYSYSARVMLMACPQLGELYKNTCRMADDASGADKVLPDKSIHFL240VGGRAKSETMALGGPWSPSLDGPDPQGDPRT 271<210>47<211>762<212>PRT<213>日本血吸虫(Schistosoma japonicum)<400>47MIRPLSSSEYTQMAGRAGRRGLDAKGYVIILVSSIGKSLKSSYTGLPTEGVLRSVILGTQ60TRLVSQFKVTYSMILNLHRSSSLTLRDVMKRSFMEAASHRWETEQRQHLSFLNKKLDETT120TLISSNDSNQTSVNSVSAFTENNDDLSESLFVYLDVRVKCPYNGIECCDSISAYYQLCYR180YRQLTQSLIRTLSTKYERYLRNIFCPGRVILLHVTIKQSVWLVPGVIVNYYWETKKNGKD240QLLFRAILWKLPTDSPTFFTKKQEGESETSAVVGSNNKISTAPVGEDNHSWYNCEYDEIE300EKILRNIKGSCNTQWEETPVPPHLVPVFMPSSLEDGYARVTLQSILPEDSLFRICDRIVT360FENKSLKLNMNISDCLLHSIKQHQNTVNRRETSVNLPDDNNEHYFPILNDVNLALFNSVN420SPQHNSSFCVIYTTLSDNVPKQVDITKELKADKIDEEDVRLFEEYSTINDQLTYPLSISD480TTTLNSNLLKCPNLLQHLNRVHQTIRRKLTIKKIETNLADYQLQLSKEYTGRLNVLKELG540FIDSATQSYCLSSKGFFACELIKKEVVLTQLLLDGFIDNLLAPDIAAVLSVFANELRPYD600FSLEKKSSSYLKELVNSIDRINSNTNYVSNNDLDLIPRHLLPVFKRILNCAYELETLQRF660HNLIDPYLESRFDLRLVPLVYKWAKGYSFSATIAKCDLPEGLLIKSLLQLDELIRHIVGA720CRQFGKHILSLKMCEARNLIYRDIVSSPSLYILKGIKSVTDD 762<210>48<211>476<212>PRT<213>日本血吸虫(Schistosoma japonicum)<400>48MERLDSLLCRLEKATIILEAAAVNSMYRTSPIKTSSESRNPLSVKEFHDIITGPLSEYVL60YSSKIGDVVKTHASVVERCFLMEEEILKLASECSKPSDSELTLIMTLLGKEIEEVVKLKD120NNRSNRFFNHLSAVAESVAALGWLSVSPAPAPYIKTMKEAAEFFTNRVIKEYEEKDTVHL180AWKKALMSVLTALELYVKKHHTTGLVWNARGKPATASKLSKSSPTREVNSNCSVALPPPP240ALTPAHLAAISLQGKNQSPQSALFSDINRGEAVTSNLRKVTDDMKTHKNPKLREGPIMHT300SRHQVNSSLNNTIIRSPAEKKGCLELKGNKWVVEYFNSVENLEVIITEPKQTVFIYKCNE360CTIRIKGKANSIMLDNCKKTGVVFDYLISSIDVVNCQSIQIQCLGKLSTVNIDKTDGCQV420FLNEDSKYADVITAKSSEVNLLVPNATGHIEEFAVPEQFKTNFTGNGLKTVCTDSR47权利要求
1.一类日本血吸虫表皮抗原基因，其编码具有日本血吸虫表皮抗原蛋白质活性的核苷酸序列，所述的核苷酸序列包括(a)SEQ ID No.1～SEQ ID No.24所示的序列；(b)SEQ ID No.1～SEQ ID No.24所示的序列中每条序列的互补序列；(c)与SEQ ID No.1～SEQ ID No.24所示的序列中每条序列有至少70％同源性的序列，及(d)上述(a)～(c)中一条或数条的组合。
2.如权利要求1所述的一类日本血吸虫表皮抗原基因的核苷酸序列，其特征在于所述的核苷酸序列包括具有SEQ ID No.1～SEQ ID No.24所示的序列。
3.一类日本血吸虫表皮抗原蛋白，包括(a)具有SEQ ID No.25～SEQ ID No.48所示氨基酸序列的蛋白；(b)由(a)的蛋白发生一个或几个氨基酸置换、缺失或插入而形成的具有(a)蛋白相同活性的蛋白。
4.如权利要求3所述的日本血吸虫表皮抗原蛋白，其特征在于，其具有SEQ IDNo.25～SEQ ID No.48所示的氨基酸序列。
5.一类抗体，其特征在于，所述抗体能与权利要求3所述的蛋白特异性结合。
6.权利要求3所述的日本血吸虫表皮抗原蛋白在制备血吸虫疫苗中的应用。
7.权利要求3所述的日本血吸虫表皮抗原蛋白在制备防治血吸虫病的药物中的应用。
全文摘要
本发明公开了一类日本血吸虫表皮抗原基因及其编码蛋白，还公开了与该日本血吸虫表皮抗原蛋白特异结合的抗体。此外，本发明还公开了此类日本血吸虫表皮抗原蛋白在制备血吸虫疫苗中的应用，以及在制备防治血吸虫病的药物中的应用。运用本发明的日本血吸虫表皮抗原蛋白，可研究血吸虫治疗药物的作用靶点，从而在开发研制预防或治疗血吸虫病的药物过程中发挥重要作用。
文档编号C07K14/44GK1986797SQ20051011171
公开日2007年6月27日 申请日期2005年12月20日 优先权日2005年12月20日
发明者刘锋, 韩泽广, 王志勤, 胡薇, 冯正 申请人:上海人类基因组研究中心, 中国疾病预防控制中心寄生虫病预防控制所