1)mismatch PCR错配PCR
1.To further validate this SNP(single-nucleotide polymorphism) locus,the technique of mismatch PCR was established and amplified to examine the samples of embryo,paternal and maternal genomic DNA.利用DNA测序技术,在荷斯坦奶牛胎儿中找到X染色体基因GLRA2 3’端的杂合位点(C/T),设计错配引物在该位点对胎儿及其父本、母本基因组进行PCR扩增,建立起GLRA2基因的错配PCR技术,该技术可以快速在群体基因组中检测这一杂合位点。
英文短句/例句
1.Detection of X-linked Heterozygous Gene in Cow with Mismatch PCR错配PCR对牛X染色体相关基因杂合子的检测
2.The Reserch of Improving Activity of Phytase by Error-prone PCR易错PCR突变提高植酸酶活力的研究
3.maturity mismatching of assets and liabilities资产与负债期限错配
4.Study on Polymerase Chain Reaction Assay for Detection of Enterobacter Sakazakii in Powder Infant Formula Milk;PCR技术检测婴儿配方奶粉中坂崎肠杆菌的研究
5.The Application of Mating Test and Degenerate PCR in Genetic Analysis of Auricularia Auricula;交配反应与简并PCR在黑木耳遗传分析中的应用
6.Application of PCR Technique in Study on the Mating Type Factor of Auricularia Auricula;PCR技术在黑木耳交配型因子研究中的应用
7.Aptamer 6-10 of DNA polymerase can increase the sensitivity of quantitative PCRDNA聚合酶适配子6-10提高定量PCR的特异性
8.One was pure edge misfit dislocations with line directions of 〈100〉;失配位错的类型分为刃型失配位错,位错线沿〈100〉方向;
9.Such an accident would yield false results, especially if PCR amplification is used.这样的偶然事件会产生错误的结果,尤其是当使用PCR扩大程序时。
10.Study on the Circumstances of Mutation of Hyperthermophilic α-Amylase from Deep-sea Archaeon through Error-prone PCR深海古菌超嗜热α-淀粉酶易错PCR致突变的条件研究
11.Evolution of interphase misfit dislocation networks in Ni-based single-crystal superalloy镍基单晶高温合金相界面错配位错网络的演化
12.Study on Real-time PCR for the Detection of Enterobacter Sakazakii in Infant Formula;荧光定量PCR检测婴儿配方奶粉中阪崎肠杆菌研究
13.Error disabling the %2 service for hardware profile %3. Configuration Manager error %4 occurred: %1错误禁用 %2 服务给硬件配置文件 %3。配置管理器错误 %4 产生: %1
14."Error enabling the %2 device for hardware profile %3. Configuration Manager error %4 occurred: %1"错误启用 %2 设备硬件配置文件 %3。配置管理器错误 %4 产生: %1
15."Error disabling the %2 device for hardware profile %3. Configuration Manager error %4 occurred: %1"错误禁用 %2 设备硬件配置文件 %3。配置管理器错误 %4 产生: %1
16."Error enabling the %2 service for hardware profile %3. Configuration Manager error %4 occurred: %1"错误启动 %2 服务给设备硬件配置文件 %3。配置管理器错误 %4 产生: %1
17.It is often desirable to apply pitch mismatch or rotational mismatch to increase the fringe density.经常希望采用间距错配或转动错配以增加条纹密度。
18.The complexity of this configuration also make mis-configuration a possibility.这种配置的复杂性也有可能产生配置错误。
相关短句/例句
mispairing PCR-RFLP错配PCR-RFLP
1.A mispairing PCR-RFLP technique was applied in this study to determine the Insulin-like Growth Factor 2(IGF2) gene intron3 G3072A mutation in an outbred Landrace and Large White, and the gelded boars from Landrace× Large White cross.采用错配PCR-RFLP技术鉴别了长白猪和大白猪纯种以及长白×大白猪杂交后代去势公猪胰岛素样生长因子2(IGF2)基因内含子3的3072位点变异,分析了遗传来自父本不同等位基因个体间的相关性状差异及不同等位基因的遗传效应。
3)mismatch-PCR错配PCR方法
1.The results showed that the mismatch-PCR method developed for gyrA and parC mutations may be a simple,specific and rapid method for detection of this important fluoroquinolone resistance mutation and provide a useful tool for clinical diagnosis or epidemiological studies.根据耐药沙门氏菌基因突变位点碱基变化情况,设计合适的引物进行错配PCR,鉴别突变位点,使其结果与测序结果相吻合,以建立错配PCR方法快速检测耐氟喹诺酮类药物沙门氏菌基因突变,判断菌株是否耐药。
4)MAMA-PCR错配扩增突变分析PCR法
5)error-prone PCR易错PCR
1.Directed evolution by error-prone PCR of Armillariella tabescens MAN47 β-mannanase gene toward enhanced thermal resistance基于易错PCR的假密环菌Armillariella tabescens MAN47 β-甘露聚糖酶耐高温定向进化
2.The aac(3)-I was reconstructed in vitro by error-prone PCR.然后在体外对aac(3)-I基因进行易错PCR改造,筛选到一株阳性克隆测序后发现7处碱基变异,其中C272T、T373C引发了氨基酸的变异,分别对应:Ser91Phe、Tyr125His。
3.Random mutagenesis on Bacillus pumilus lipase YZ02 gene was conducted by using error-prone PCR strategy.利用易错PCR技术对短小芽胞杆菌(Bacillus pumilus)YZ02脂肪酶基因BpL进行两轮定向进化研究,分别获得最佳突变株BpL1-7和BpL2-1369,其脂肪酶活力比出发酶分别提高了2倍和6倍。
6)random mutagenesis PCR致错PCR
1.Methods: AOX1 promoter mutants were constructed by random mutagenesis PCR.方法:通过致错PCR构建醇氧化酶1启动子突变体,经酶切连接到改造过的质粒HSA-pPIC9上,转化毕赤酵母GS115感受态细胞,摇瓶培养表达筛选人血清白蛋白(HSA)高表达突变体菌株。
延伸阅读
密码错配分子式:CAS号:性质: 在DNA的全长上存在的非互补的碱基,而本来应该是碱基对。
