逆转录酶聚合酶链反应,Reverse transcription polymerase chain reaction英语短句,例句大全

逆转录酶聚合酶链反应,Reverse transcription polymerase chain reaction
1)Reverse transcription polymerase chain reaction逆转录酶聚合酶链反应
2)Reverse transcriptase polymerase chain reaction逆转录酶聚合酶链反应
3)reverse transcription polymerase chain reaction逆转录聚合酶链反应
1.[Methods] The level of human Fascin1 gene in endometrial carcinoma tissues(study group,n=30) and normal endometrial tissues(control group,n=10) was investigated by reverse transcription polymerase chain reaction.方法选取30例新鲜子宫内膜癌组织标本(实验组)及10例正常子宫内膜组织标本(对照组),应用逆转录聚合酶链反应(RT-PCR)研究人类Fascin1基因的表达情况,并分析其与组织学类型、临床分期、病理分级、雌孕激素受体状态的相关性。
2.After 1,4,7,10,14 days of exposure,the rats were killed,whole lung RNA were isolated and ATPase6,8 mRNA were detected by reverse transcription polymerase chain reaction(RT-PCR).二组分别于实验后1、4、7、10和14 d提取其肺组织RNA,采用半定量逆转录聚合酶链反应(RT-PCR)测定ATPase6、8 mRNA表达。
3.Methods COX-2 mRNA expression was determined by semi-quantitative reverse transcription polymerase chain reaction(RT-PCR),and cell proliferation analyzed by cell counting kit-8(CCK-8) assay after exposure to dicl.方法采用半定量逆转录聚合酶链反应(RT PCR)检测各种细胞系COX 2 mRNA的表达水平,采用cell counting kit 8(CCK 8)细胞计数法测定药物作用72 h后的细胞增殖活性,绘制生长曲线。

英文短句/例句

1.Dection of enterovirus infection by reverse transcription and polymerase chain reaction应用逆转录聚合酶链反应检测肠道病毒感染
2.Rapid identification of subtypes of respiratory syncytial virus by real-time reverse transcription PCR实时逆转录聚合酶链反应快速鉴别呼吸道合胞病毒亚型
3.Research of Real-time Quantitative Reverse-transcriptase Polymerase Chain Reaction (RQ-PCR) to Detect bcr/abl Fusion Gene in Chronic Myeloid Leukemia;实时定量逆转录聚合酶链反应检测慢粒bcr/abl融合基因的研究
4.Expression of the multidrug resistance in human bladder tumors by reverse transcription polymerase chain reaction应用逆转录聚合酶链反应技术检测膀胱癌多项耐药基因的表达
5.Analysis of reserved conditions of blood sample for detection of HCV RNA with RT-PCR逆转录聚合酶链反应检测HCV RNA血标本保存条件分析
6.Comparison Study of Conventional Method and Reverse Transcription Polymerase Chain Reaction on Detection of Flavivirus in Mosquitoes逆转录聚合酶链反应与常规法检测蚊体内黄病毒的比较研究
7.DETECTION OF FISH VIRAL HEMORRHAGIC SEPTICEMIA VIRUS (VHSV) BY NESTED REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION (RT-PCR)应用巢式逆转录聚合酶链反应检测鱼类病毒性出血性败血症病毒(VHSV)
8.Clinical value of antenatal diagnosis of intrauterine active human cytomegalovirus infection with reverse transcription polymerase chain reaction逆转录聚合酶链反应技术用于产前诊断人巨细胞病毒宫内活动性感染的临床价值
9.Establishment of reverse transcription semi-nested polymerase chain reaction to the pathogenic diagnosis of JE patients逆转录-聚合酶链反应在乙型脑炎诊断中的建立
10.Detection of Rice Stripe Virus by Reverse Transcription and Polymerase Chain Reaction逆转录聚合酶链式反应快速检测水稻条叶枯病毒
11.DETECTION OF ENTEROVIRUS RNA IN HUMAN BLOOD SPECIMENS BY REVERSETRANSCRIPION POLYMERASE CHAIN REACTION逆转录聚合酶链式反应检测人血标本中肠道病毒核糖核酸
12.Detection of mRNA in kidney of dead rat using fluorescence semi-quantitative RT-PCR;荧光半定量逆转录-聚合酶链式反应检测大鼠死后肾mRNA
13.Development of a SYBR Green Real-time RT-PCR for Detection of Mumps Virus GeneSYBR Green荧光逆转录-聚合酶链反应检测流行性腮腺炎病毒基因
14.Reverse transcription multiplex-nPCR:application in the study on causative organisms of viral encephalitis in children逆转录多重套式聚合酶链反应在儿童病毒性脑炎病原学研究中的应用
15.Multiplex Reverse Transcription-nPCR: Application in the Study of Causative Organisms of Viral Encephalitis in Children;多重逆转录套式聚合酶链式反应在儿童病毒性脑炎病原学研究中的应用
16.The detection of circulating hepatocellular carcinoma cells in peripheral venous blood by reverse transcription polymerase chain reaction and its clinical significance巢式逆转录多聚酶链反应检测循环性肝癌细胞及其临床意义
17.Studies on the Detection of Bovine Viral Diarrhea-mucosal Disease Virus Using Reverse Transcription-nested Polymerase Chain Reaction;反转录—套式聚合酶链式反应检测牛病毒性腹泻/粘膜病病毒的研究
18.Studies on the Detection and Differentiation of Virulent and Avirulent Strains of Newcastle Disease Virus in Chickens Using Reverse Transcription-Nested Polymerase Chain Reaction;反转录—套式聚合酶链反应检测、鉴别鸡新城疫病毒强、弱毒株的研究

相关短句/例句

Reverse transcriptase polymerase chain reaction逆转录酶聚合酶链反应
3)reverse transcription polymerase chain reaction逆转录聚合酶链反应
1.[Methods] The level of human Fascin1 gene in endometrial carcinoma tissues(study group,n=30) and normal endometrial tissues(control group,n=10) was investigated by reverse transcription polymerase chain reaction.方法选取30例新鲜子宫内膜癌组织标本(实验组)及10例正常子宫内膜组织标本(对照组),应用逆转录聚合酶链反应(RT-PCR)研究人类Fascin1基因的表达情况,并分析其与组织学类型、临床分期、病理分级、雌孕激素受体状态的相关性。
2.After 1,4,7,10,14 days of exposure,the rats were killed,whole lung RNA were isolated and ATPase6,8 mRNA were detected by reverse transcription polymerase chain reaction(RT-PCR).二组分别于实验后1、4、7、10和14 d提取其肺组织RNA,采用半定量逆转录聚合酶链反应(RT-PCR)测定ATPase6、8 mRNA表达。
3.Methods COX-2 mRNA expression was determined by semi-quantitative reverse transcription polymerase chain reaction(RT-PCR),and cell proliferation analyzed by cell counting kit-8(CCK-8) assay after exposure to dicl.方法采用半定量逆转录聚合酶链反应(RT PCR)检测各种细胞系COX 2 mRNA的表达水平,采用cell counting kit 8(CCK 8)细胞计数法测定药物作用72 h后的细胞增殖活性,绘制生长曲线。
4)Reverse transcription-polymerase chain reaction逆转录-聚合酶链反应
1.Expression of the TFPI-2 gene was determined by Reverse transcription-polymerase chain reaction(RT-PCR) and Western blotting.方法:将重组质粒pEGFP-C1-TFPI-2通过脂质体介导转染胰腺癌细胞系Panc-1细胞,G418筛选获得阳性细胞克隆后,用逆转录-聚合酶链反应(RT-PCR)和免疫印迹(Western blot)技术分别检测转染细胞中TFPI-2 mRNA及相应蛋白的表达,同时测定转染细胞的生长曲线和凋亡情况。
2.Methods:Reverse transcription-polymerase chain reaction(RT-PCR) was used to semiquantite the expressions of GRα and β mRNA in PBMC of 18 cases of PM/DM patients sensitive to GC,10 cases of PM/DM patients resistant to GC and 20 cases of health blood donors.方法采用逆转录-聚合酶链反应(RT-PCR)方法对18例GC治疗敏感、10例GC治疗抵抗的PM/DM患者PBMC中GRα、β mRNA的表达水平进行了检测,同时采用放射免疫分析法测定血清中的皮质醇含量,并与20例正常对照进行比较。
3.Methods Using reverse transcription-polymerase chain reaction(RT-PCR),the PIM3 mRNA expression in normal murine ocular tissues was defected.方法应用逆转录-聚合酶链反应及免疫组化检测PIM3在小鼠眼部组织中的表达。
5)Reverse transcriptase polymerase chain reaction逆转录聚合酶链反应
1.Methods The p24 gene fragment of BDV in PBMCs or CSFs in these patients infected in intracalvarium were examined by nested reverse transcriptase polymerase chain reaction(RT-PCR) and .方法用荧光定量巢式逆转录聚合酶链反应(FQ-nRT-PCR)方法检测不明原因颅内感染患者及对照者PBMCs或CSFMCs中BDV p24基因片段,同时用β-肌动蛋白(-βactin)作为内参照,并总结阳性患者的临床特征。
2.Methods: The p24 fragment of BDV in peripheral blood mononuclear cells(PBMC) in 52 patients with viral encephalitis and 30 healthy donors were examined by nested reverse transcriptase polymerase chain reaction(RT-PCR) and fluorescence quantitative(FQ) PCR.方法:用荧光定量巢式逆转录聚合酶链反应(FQ-n RT-PCR)方法检测病毒性脑炎患者及正常体检者PB-MCs中BDVp24基因片段,同时用β-肌动蛋白(β-actin)作为内参照,总结出临床特征。
3.The two consecutive fragments of human U5·116 ku were amplified by reverse transcriptase polymerase chain reaction (RT-PCR).方法:从HeLa细胞中提取总体RNA,一步法合成单链cDNA,利用逆转录聚合酶链反应(RT-PCR)法,扩增出人类U5·116 ku基因两段连续的序列,首先分别克隆至pTZ57R/T载体,两段序列连接成全长后再定向克隆至真核表达载体pcDNA3。
6)RT-PCR逆转录-聚合酶链反应
1.Methods:Reverse transcription polymerase chain reaction(RT-PCR) was used to detect the expression of RASSF1A mRNA in 29 cases of human transitional cell carcinoma of bladder and 5 cases of normal bladder tissues.方法:采用逆转录-聚合酶链反应(RT-PCR)检测29例膀胱移行细胞癌组织及5例正常膀胱组织中RASSF1A mRNA的表达水平。
2.Results:RT-PCR and the sequence analysis of its product suggested that these 58 strains of measles viruses should belong to the H1 genotype of the eighth genetic group of wild measles virus.结果:该58株病毒经逆转录-聚合酶链反应和其产物的基因序列分析显示,这58株病毒属于麻疹野病毒第8基因组H1基因型。
3.Methords: The expression of PCA-1 mRNA was detected by RT-PCR in the samples from 45 cases of PCa with various clinico-pathologic characteristics, 30 cases of high-grade prostatic intraepithelial neoplasia (HG-PIN), 43 cases of BPH and 39 cases of other carcinorma tissues.方法:采用逆转录-聚合酶链反应(RT-PCR)技术,检测45例PCa组织、30例前列腺高分级上皮样内瘤样病变组织(HG-PIN)、43例BPH组织和39例其他肿瘤组织标本中PCA-1 mRNA的表达。