1)CHO-dhfr-cellCHO-dhfr-细胞
1.After tranfection with CHO-dhfr- cell line, fibrinolysis activity was detected in the cultivate supernatant.利用携带有二氢叶酸还原酶(dhfr)基因的pCI载体,实现tPA突变体(FrGGI)在CHO-dhfr-细胞中的高效表达,获得高表达细胞株。
2.AIM: To obtain high level expression of recombinant human truncated osteoprotegerin(TOPG) with higher bioactivity in CHO-DHFR-cells.1/DHFR-hTOPG,实现其在CHO-DHFR-细胞中的高效表达,获取生物活性较高的重组蛋白。
2)CHO/dhfr~-cellCHO/dhfr-细胞
1.AIM: To obtain CHO/dhfr~- cells line with integrated FRT sequence in the chromosome transcription active site and to express human-mouse chimeric antibody directed against Chikungunya Virus by using the cell line.以此载体转染CHO/dhfr-细胞,用1g/L的G418筛选抗性克隆。
英文短句/例句
1.Stable Expression of Bovine Prion Protein in CHO/dhfr-cells牛朊蛋白在CHO/dhfr-细胞中的稳定表达
2.The Cloning of HN Gene of Newcastle Disease Virus and Expression in CHO/dhfr-cells新城疫病毒HN基因的克隆及其在CHO/dhfr-细胞中的表达
3.Expression of Genetically Engineered Anti-Keratin Full-Length Human IgG in CHO(dhfr～-) Cells;基因工程抗角蛋白抗体全长人IgG在CHO（dhfr～-）细胞的表达
4.Expression of truncated fragment of human OPG in CHO-DHFR~- cells and its bioactivity characterization截短型人骨保护素在CHO-DHFR~-细胞中的表达及活性测定
5.Improvement of Anti-HBsAg Antibody Production in CHO Cell;抗HBsAg抗体在CHO细胞中表达量提高研究
6.The Chinese Hamster Ovary Cells Apoptosis Induced by X-ray Irradiation;电离辐射致中国仓鼠卵巢（CHO）细胞凋亡
7.Construction of CHO Cells Stably Expressing CCR5;CCR5稳定表达的CHO细胞的构建
8.Construction of CHO Cell Line Stably Expressing Mouse Lamada-Interferon稳定表达鼠IFN-λ的CHO细胞系建立
9.Optimization of Manufacturing Process of Recombinant CHO Cell Hepatitis B Vaccine重组CHO细胞乙肝疫苗生产工艺的优化
10.Expression of Full-length MBL Gene of Han Chinese in CHO Cells汉族人MBL全长基因在CHO细胞中的表达
11.Primarily Exploring on Serum-low Culture of Engineering Cells of CHOCHO工程细胞株低血清培养的初探
12.Optimization of Gene Transient Transfection in CHO-K1 CellCHO-K1细胞中基因瞬时转染的条件优化
13.Purification and Activity of Recombinant Human IL-12(CHO Cells)重组人白细胞介素-12(CHO细胞)的纯化及其活性
14.Stable Expression of the Recombinant Human Membrane Form Klotho Protein in CHO Cells;重组人膜型Klotho蛋白在CHO细胞中的稳定表达
15.Study on High-level Expression of Recombinant Hepatitis B Surface Antigen in CHO Cell;重组CHO细胞高效表达乙肝表面抗原的研究
16.The Expression of CTLA-4/IgG4 Fusion Protein at High Level in CHO Cells;CTLA-4/IgG4融合蛋白在CHO/DG44细胞中的高效表达
17.Stable Expression of the Human Secreted Form Klotho Protein in CHO Cells;人分泌型KL蛋白在CHO细胞中的稳定表达
18.Manufacture of Collagen Carriers and Its Application in CHO Cell Culture;胶原蛋白载体制备及在CHO细胞培养中的应用
相关短句/例句
CHO/dhfr~-cellCHO/dhfr-细胞
1.AIM: To obtain CHO/dhfr~- cells line with integrated FRT sequence in the chromosome transcription active site and to express human-mouse chimeric antibody directed against Chikungunya Virus by using the cell line.以此载体转染CHO/dhfr-细胞,用1g/L的G418筛选抗性克隆。
3)CHO-dhfr-CHO-dhfr细胞
4)CHO dhfr cellCHO-dhfr~-细胞
5)CHO dhfr-cellsCHO dhfr~-细胞
6)CHO/dhfrCHO/dhfr-
1.Results: The bioinformatic analysis indicated BQ135232 possessed the possibility to be an immunogen, subsequently we constructed the expressing vector pcDNA3-BQ-ORF-GPI, transfected CHO/dhfr-successfully, and verified by RT-PCR.方法:应用生物信息学方法分析BQ135232 mRNA基因序列,初步了解其基本性状,构建表达载体,转染真核细胞(CHO／dhfr-)。
延伸阅读
CHO细胞分子式：CAS号：性质：一个转化细胞系，1957年从中国地鼠卵巢细胞得到。广泛采用的K1亚克隆株需要脯氨酸，因为遗传学上有缺陷，不能将谷氨酸转变为谷氨酸-γ-半醛。该细胞是上皮样细胞，通常贴壁生长，也可悬浮生长。CHO细胞被广泛地用来表达重组DNA的蛋白。
