电穿孔转染,electroporation
1)electroporation电穿孔转染
1.Objective To optimize the electroporation parameters in mouse primary lymphocyte cultured in suspension with adenosine deaminase acting on RNA 1(ADAR1) as a reporter gene.目的以RNA依赖性腺嘌呤脱氨酶(ADAR1)为靶基因优化悬浮培养的小鼠原代淋巴细胞的电穿孔转染条件。
2.It turns out that 250mg/L carrier DNAincreases the expression level by 3 fold,andlinearized plasmid DNA is more suitable thansupercoiled DNA to obtain stable transfectionby electroporation.以外源红细胞生成素cDNA的表达产物为指标,研究了运载DNA和重组表达质粒的构象对电穿孔转染CHO细胞的效率的影响。
英文短句/例句

1.The Isolating Culture of Neural Stem Cells from Rat's Fetus Brain and Transfection by Electroporation大鼠胎脑神经干细胞的分离培养及电穿孔转染
2.Study on factors of the transfection efficiency and the optimum conditions for the sperm-mediated gene transfer by electroporation in goats;山羊精子电穿孔转染外源DNA影响因素及最适条件的研究
3.Transfection of mature dentritic cells with whole RNA from colon adenocarcinoma cells by electroporation in vitro结肠癌细胞总RNA电穿孔法体外转染成熟树突状细胞
4.Macromolecule Transport Through Epidermis by Electroporation;大分子经皮转运的电穿孔技术的研究
5.Electroporation Enhanced Intramuscular Transferring of a Doxycycline-Regulated Human Proinsulin Expression Plasmid and Its Hypoglycemic Effect on STZ-induced Diabetic Mice;电穿孔介导的可调控人胰岛素基因肌肉转染对糖尿病小鼠的降糖作用
6.Rescue of Photoreceptors by BDNF Gene Transfer Using in Vivo Electroporation in the RCS Rat of Retinitis Pigmentosa在体电穿孔辅助BDNF基因眼内双腔转染延缓RCS大鼠视网膜色素变性的实验研究
7.Basic fibroblast growth factor gene transferred into retinal ganglion cells by in vivo electroporation prevents photoreceptor degeneration in RCS rats电穿孔辅助bFGF基因转染视网膜神经节细胞层延缓RCS大鼠视网膜色素变性
8.An input unit that reads or senses the holes in a punched card, transforming the data from the hole patterns to electric signals.一种输入装置,它读取或检测穿孔卡上的孔,把以孔的模式表示的数据转换成电信号。
9.Influence of resistance of TiO_2 mesoporous films on photoelectric properties of dye-sensitized solar cellsTiO_2介孔薄膜电阻对染料敏化太阳能电池光电转换性能的影响
10.reeves electronic analog computer穿孔电子模拟计算机
11.hole-conductor current access device穿孔导体电流存取器件
12.brush compare chock电刷(穿孔)比较检查
13.Research on Electroporation Transfection Chlamydia Trachomatis to Build Vector of Chlamydia;电穿孔转化沙眼衣原体建立衣原体载体实验研究
14.Experimental Studies on Generation of Transgenic Mice by Intratesticular Injection and in Vivo Electroporation;睾丸内注射和体内电穿孔法建立转基因小鼠的实验研究
15.Preliminary Studies on Foreign DNA Delivery to Penaeus chinensis with Gene Gun and Electroporator;利用基因枪和电穿孔仪将外源DNA转入中国对虾的初步研究
16.The establishment of genetic transformation system in lateral cortex of mouse embryo via in utero electroporation鼠胚大脑皮层子宫内电穿孔法基因转移体系的建立
17.A severe infection may cause the appendix to rupture.严重的感染可能导致阑尾穿孔。
18.punched tape to magnetic tape converter穿孔纸带到磁带的转换器
相关短句/例句

Electroporation电穿孔转化
1.After electroporation of the plasmid into Lactobacillus casei CECT5276, PCR using the genomic DNA of the recombinant lactobacillus as template was performed to confirm whether t.方法 :用 PCR从短小乳酸杆菌染色体中扩增得到 S-层蛋白的信号肽序列 ,将其与 h CGβ基因连接后克隆到乳酸杆菌整合型表达载体 p Ilac的 lac启动子下游 ,得到质粒 p Ilac h CGβ,电穿孔转化干酪氏乳酸杆菌 Lb。
2.Conjugation and electroporation were used to genetically improve these strains.利用接合转移和电穿孔转化等方法对这些菌株进行了遗传改良研究。
3)electroporational gene transfer电穿孔基因转移
4)electroporation电穿孔
1.Characteristic analysis of electroporation apparatus for transdermal drug delivery;经皮给药电穿孔仪的性能分析
2.Progress of electroporation in improving the efficacy of DNA vaccine;电穿孔增强核酸疫苗免疫反应的研究进展
3.Effect of Electric Field Direction on the Percutaneous Penetration of Caffeine Mediated by Skin Electroporation and Iontophoresis;电场方向对咖啡因电穿孔与离子导入经皮渗透的影响比较
5)electroporation电穿孔法
1.Experimental research on transformation of plasmid DNA into Escherichia coli XL1-Blue MRF′ strain mediated by electroporation.;电穿孔法介导质粒DNA转化大肠杆菌XL1-Blue MRF′菌株的实验研究
2.The transfection efficiency of human telomerase reverse transcriptase(hTERT) in primary culture of neonatal rat cardiomyocytes is improved by electroporation;电穿孔法提高hTERT转染原代培养乳鼠心肌细胞效率的研究
3.Optimization of parameters of transfection of primary cultured keloids fibroblasts in vitro by electroporation;电穿孔法转染原代培养的瘢痕疙瘩成纤维细胞的条件优化
6)piercing by electric arc电弧穿孔
延伸阅读

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