Red重组,Homologous-recombination英语短句,例句大全

Red重组,Homologous-recombination
1)Homologous-recombinationRed重组
1.Homologous-recombination between linear DNA cassettes and.将其用PvuⅡ酶切 ,回收含ptsG1 cat sdh ptsG2 的目的片段 ,电转化至JM10 9 pKD4 6 ,在Red重组酶的作用下 ,外源DNA片段与染色体上对应区域发生同源重组 ,将基因ptsG敲除 ,替换为cat sdh基因 ,获得整合sdh基因的JM10 9s。

英文短句/例句

1.Construction of a dsRNA Prokaryotic Expression System Using Red Recombination for Resistance to Tobacco Mosaic Virus利用Red重组系统构建dsRNA原核表达体系抗烟草花叶病毒的研究
2.Research on Construction of BAC Library of Guanzhong Milk Goat and Homologous Recombination with Red System;关中奶山羊BAC文库构建及其利用Red系统同源重组的研究
3.Construction and Identification of 3-Amino-5-hydroxybenzoic Acid(AHBA) Biosynthesis Gene Cluster Targeting Vector in Streptomyces by Red/ET RecombinationRed/ET重组AHBA生物合成基因簇打靶载体的构建与鉴定
4.Knockout of the Crystalline Inclusion Protein Gene cipA and cipB in Photorhabdus luminescens by RED Homologous RecombinationRED同源重组法敲除发光光状杆菌晶体蛋白基因cipA和cipB
5.Comparison of two methods for constructing Salmonella enteritidis mutants:Red recombination system and suicide vector system基于Red同源重组和高效自杀性载体系统构建肠炎沙门氏菌突变株方法的比较
6.The Appreciation of the translation of A Red, Red Rose;A Red,Red Rose译文赏析
7.RED HAT LINUX INSTALLATION;Red Hat Linux的安装
8.Construction of Bacterial Artificial Chromosome Vectors Containing Human Tissue-type Plasminogen Activator Mutant Gene Specially Expressed in Mammary Gland with Red Recombination System;利用Red系统构建人组织型纤溶酶原激活剂突变体BAC乳腺特异表达载体
9.FERED:Fairness Enhancement REDFERED:公平性增强的RED算法
10.On Gadamer s "Horizon Fusion" -A Comparative Study on Two Chinese Versions of "A Red,Red Rose";从“A Red,Red Rose”的两种译本谈伽达默尔的“视界融合”
11.`red' is an attributive adjective in `a red apple'.‘red（红）’在‘a red apple（一个红苹果）’中是一个作定语的形容词。
12.'red' is an attributive adjective in 'a red apple'.'red（红）’在‘a red apple（一个红苹果）'中是一个作定语的形容词。
13.Notion of "Transformation" in poem translation --Commentary on WANG Zuo-liang s translation of A Red, Red Rose;诗歌翻译与“化境”论——兼评王佐良的A Red,Red Rose英诗汉译
14.Installing Windows2000 and Red Hat Linux9 in a Computer在一台计算机上安装Windows2000与Red Hat Linux9
15.You can't believe how much red tape there is in getting a visa.下面我们再来举一个有关red tape的例子。
16.Installing Oracle 9i on Red Hat Advanced ServerOracle 9i在Red Hat Advanced server下的安装
17.Key Technology about Creating and Running Apache on Red Hat LinuxApache在Red　Hat　Linux上建立与运行的关键技术
18.Congestion Avoidance Control and RED Algorithm Modification Research;网络拥塞控制及RED算法改进策略研究

相关短句/例句

Red recombination systemRed重组酶
3)Red/ET recombinationRed/ET重组
1.For study of the function of new gene-Resp18,we brought in a new DNA engineering platform-Red/ET recombination to construct Resp18 targeting vector.研究表明,Red/ET重组方法构建打靶载体具有很高的效率,可以获得较长的同源臂,并且不会引入突变,有助于获得更高的打靶效率。
2.A rapid and high efficient working system for gene-targeting vector construction was developed by using Red/ET recombination.通过合理应用Red/ET重组技术实现基因打靶载体的快速构建。
3.The results showed that Red/ET recombination has the advantage of high efficiency and time saving for construction of the targeting vector in studies of the gene function in streptomyces.研究显示了Red/ET重组工作效率高、操作简单、精确的优点,可大大缩短构建打靶载体的时间。
4)Red recombinant systemRed重组系统
1.The global regulatory gene csrA was disrupted and replaced by multigene cassette using Red recombinant system.利用Red重组系统 ,在破坏整体调控基因csrA时 ,替换多基因盒。
2.With the help of Red recombinant system, asd genes of DH5α and 2457T were in vivo replaced by chloramphenicol gene.方法 :本研究首先合成一对引物 (每一条的 5′端与asd基因同源 ,3′端与氯霉素抗性基因同源 ) ,并用来获得氯霉素抗性基因的PCR产物 ,然后电击转化至大肠杆菌DH5α和痢疾杆菌福氏 2a 2 4 5 7T中 ,在λRed重组系统的帮助下 ,通过氯霉素抗性基因两侧的asd基因序列在体内与asd基因发生同源重组 ,置换了DH5α和 2 4 5 7T基因组中的asd基因 ,最后又利用氯霉素抗性基因两端的FRT位点 ,通过FTP位点专一性重组将氯霉素抗性基因剔除。
5)Red recombinationRed同源重组
1.The Escherichia coli strain SQ88 was selected as the target strain,to which five of rRNA operons(rrnA,rrnD,rrnE,rrnG and rrnH)were sequentially inactivated using λ Red recombination system.以1株染色体上的5个rRNA操纵元(rrnA、rrnD、rrnE、rrnG和rrnH)被敲除的大肠杆菌(Escherichia coli)菌株SQ88为出发菌,运用λRed同源重组系统和卡那霉素抗性基因筛选重组子,并利用抗性基因两端的FRT位点通过位点专一性重组将抗性基因去除,最终成功构建了1株仅具有单拷贝rRNA操纵元(rrnB)的E。
6)red recombination systemRed重组系统
1.flexneri 2a 2457T,the yciD gene was deleted by using Red recombination system,and confirmed by PCR and SDS-PAGE.方法:根据弗氏2a志贺氏菌2457T株基因组全序列,采用Red重组系统对yciD基因进行缺失,并经PCR和SDS-PAGE证实;对野生株和突变株的生长状态及生化反应进行比较研究。
2.Recently a Red recombination system was developed.最近建立了Red重组系统,该系统由3个蛋白组成:α蛋白(即λ核酸外切酶),β蛋白,Gam蛋白。
3.Methods:Based on the whole genome sequence of O157∶H7 EDL933,the z3672 gene was knocked out using the Red recombination system mediated by pKOBEG.方法:根据已知的O157∶H7 EDL933基因组全序列,采用pKOBEG介导的Red重组系统,筛选卡那霉素抗性基因替换z3672基因的阳性克隆,然后FLP位点特异性重组去除两端带有FRT位点的卡那霉素抗性基因,最后通过PCR和测序手段证实。