1)TAT-EGFP穿膜肽-增强型绿色荧光蛋白
2)Enhanced green fluorescent protein(EGFP)增强型绿色荧光蛋白
1.Objective Making the fusion protein of IgG-binding peptide with enhanced green fluorescent protein(EGFP) and determining its bioactivity.目的构建、表达增强型绿色荧光蛋白(EGFP)与IgG抗体亲和肽融合蛋白,并对其生物学功能进行研究。
2.Plasmid DNA was the enhanced green fluorescent protein(EGFP)vector.以质粒DNA为增强型绿色荧光蛋白 (EGFP)的载体 ,对处于快速增殖期的 8株猪胚胎生殖 (EG)细胞 (4～ 9代 )进行转基因试验。
3.The coding sequence of enhanced green fluorescent protein(EGFP)was cloned into the promoterless vector pLAFR6,generated the reporter plasmid pLZY.将增强型绿色荧光蛋白基因编码序列克隆到不含启动子的载体pLAFR6上,构建报告质粒pLZY。
英文短句/例句
1.Effect of Dual Promoter on Expression of Enhancement Green Fluorescent Protein双启动子对增强型绿色荧光蛋白表达的影响
2.Expression of Enhanced Green Fluorescent Protein (eGFP) in Synechocystis sp. PCC6803增强型绿色荧光蛋白在集胞藻6803中的表达
3.Construction of the mutant of human amyloid precursor protein and enhanced fluorescence protein fusion gene突变型人APP基因与增强型绿色荧光蛋白基因融合载体的构建
4.Breeding and EGFP Labeling of High Efficient Anthracene-Degrading Bacteria;蒽高效降解菌的选育及其增强型绿色荧光蛋白标记
5.Construction and Expression of the Enhanced Green Fluorescent Protein Reporter Gene Eucaryotic Vcetor Carrying BMP2 Gene;含增强型绿色荧光蛋白的BMP2真核载体的构建和表达
6.Establishment of bladder cancer cell line expressing enhanced green fluorescent protein gene稳定高表达增强型绿色荧光蛋白基因膀胱癌细胞株的构建
7.In vitro Differentiation of Enhanced Green Fluorescence Protein Gene (EGFP) Transfected Porcine Amniotic Fluid-derived Stem Cells转增强型绿色荧光蛋白基因(EGFP)猪羊水干细胞的体外分化
8.Tracking Marrow Mesenchymal Stem Cells by Transfection with Enhanced Green Fluorescent Protein增强型绿色荧光蛋白-C_1转染示踪骨髓间充质干细胞
9.Construction and identification of a screening vector using enhanced green fluorescent protein(EGFP) as an indicator利用增强型绿色荧光蛋白基因作筛选标记克隆载体的构建及鉴定
10.Construction and Expression of Enhanced Green Fluorescent Protein and FXR1 Co-expression VectorFXR1基因与增强型绿色荧光蛋白融合载体的构建及表达
11.Study on liposome-mediated enhanced green fluorescent protein expression in murine Müller cells脂质体介导增强型绿色荧光蛋白基因在鼠视网膜Müller细胞中的表达
12.Construction, Expression of Fusion Protein A-enhanced Green Fluorescent Protein (PA-EGFP) and Its Application to Immunoassay;蛋白A-增强型绿色荧光蛋白融合基因的构建、表达及其在免疫测定中的应用研究
13.Migration and Differentiation of Mesenchymal Stem Cells Labeled with Enhanced Green Fluorescent Protein in the Parkinson's Disease Rat Brain增强型绿色荧光蛋白标记骨髓间充质干细胞移植对帕金森病大鼠模型的治疗作用
14.Experimental Study of Transfecting EGFP Gene into Osteoblasts Derived from Rabbit Marrow Stromal Cells;增强型绿色荧光蛋白基因转染兔骨髓基质细胞诱导的成骨样细胞的实验研究
15.Heterogenous Expression of Enhanced Green Flurescent Protein (EGFP) Gene in Schistosoma Japonicum and in Vitro Primary Cultured Cells of Schistosomula;增强型绿色荧光蛋白（EGFP）基因在日本血吸虫体内及原代培养细胞内的异源表达
16.Constrction of Shuttle Vector Plasmid Expressing EGFP of Pseudorabies Virus-Fa Strain;含增强型绿色荧光蛋白（EGFP）的伪狂犬病病毒Fa株转移载体质粒的构建
17.Transplantation of Mesenchymal Stem Cells Labeled with Enhanced Green Fluorescent Protein into the Parkinson's Disease Rat增强型绿色荧光蛋白标记骨髓间充质干细胞移植治疗帕金森病大鼠
18.Experimental Study on the Induced Differentiation of Bone Marrow Mesenchymal Stem Cells Labelled Through Transfection of Enhanced Fluorescence Protion into Cardiomyocyte-like Cell in Vitro增强型绿色荧光蛋白标记骨髓间充质干细胞体外诱导分化成心肌样细胞研究
相关短句/例句
Enhanced green fluorescent protein(EGFP)增强型绿色荧光蛋白
1.Objective Making the fusion protein of IgG-binding peptide with enhanced green fluorescent protein(EGFP) and determining its bioactivity.目的构建、表达增强型绿色荧光蛋白(EGFP)与IgG抗体亲和肽融合蛋白,并对其生物学功能进行研究。
2.Plasmid DNA was the enhanced green fluorescent protein(EGFP)vector.以质粒DNA为增强型绿色荧光蛋白 (EGFP)的载体 ,对处于快速增殖期的 8株猪胚胎生殖 (EG)细胞 (4～ 9代 )进行转基因试验。
3.The coding sequence of enhanced green fluorescent protein(EGFP)was cloned into the promoterless vector pLAFR6,generated the reporter plasmid pLZY.将增强型绿色荧光蛋白基因编码序列克隆到不含启动子的载体pLAFR6上,构建报告质粒pLZY。
3)Enhanced green fluorescent protein增强型绿色荧光蛋白
1.Construction of expression vectors of enhanced green fluorescent protein gene and human extracellular 1-3domain of vascular endothelial growth factor(VEGF) receptor KDR gene;增强型绿色荧光蛋白基因与人血管内皮生长因子受体KDR基因胞外1-3区域融合表达载体的构建
2.Cell-penetrating peptide PEP-1 mediated transmembrane delivery of enhanced green fluorescent protein in vivo of mouse;细胞穿透肽PEP-1介导增强型绿色荧光蛋白在小鼠体内跨膜转导
3.Cell-penetrating Peptide PEP-1-mediated Transduction of Enhanced Green Fluorescent Protein into Human Aortic Smooth Muscle Cells;PEP-1介导增强型绿色荧光蛋白转导入人主动脉平滑肌细胞
4)Enhanced green fluorescent protein (EGFP)增强型绿色荧光蛋白
1.Objective To construct a novel enhanced green fluorescent protein (EGFP) tagged insect-baculovirus transference system and prepare EGFP by Sf-9 cells.目的构建可表达增强型绿色荧光蛋白(EGFP)融合蛋白的昆虫杆状病毒转移载体,利用Sf-9细胞表达、制备EGFP。
2.Objective To construct a novel enhanced green fluorescent protein (EGFP) tagged Hela cell subline.目的构建增强型绿色荧光蛋白(EGFP)标记的Hela细胞系。
5)Enhanced green fluorescent protein C3增强型绿色荧光蛋白C3
6)enhanced green fluorescence protein增强型绿色荧光蛋白
1.AIM: To construct the fusion gene of Hsp65 of Mycobacterium tuberculosis H37Rv and enhanced green fluorescence protein (EGFP) and prepare dendritic cell (DC) vaccine.目的: 构建结核杆菌H37Rv株Hsp65与增强型绿色荧光蛋白 (EGFP)的融合基因pEGHsp65, 并以其转染小鼠的树突状细胞 (DC), 制备抗结核的DC疫苗。
2.4 kb fragment near the E4 region of QU virus genome was amplified by PCR to construct a plasmid pADGFP, in which ORF1, ORF8 and ORF9 was replaced with a system expressing enhanced green fluorescence protein.4kb片段,插入来自pEGFP-C1质粒的增强型绿色荧光蛋白(EGFP)基因表达盒片段,构建了含EGFP基因的重组质粒pADGFP。
3.In order to study the separation capacity of MCARM in the extraction of expressed proteins, we investigated the extraction properties of recombinant 6×His Enhanced green fluorescence protein (EGFP) by MCARM.基于此,本研究室前人开发出了新型的金属螯合亲和反胶团(MCARM),为了验证此反胶团对实际表达蛋白的萃取分离能力,本文利用MCARM对基因工程表达产物6×his标记的增强型绿色荧光蛋白(EGFP)的萃取特性进行了系统的分析。
延伸阅读
增强型与耗尽型金属-氧化物-半导体集成电路 耗尽型MOS晶体管用作负载管，增强型MOS晶体管用作驱动管组成反相器（图1），并以这种反相器作为基本单元而构成各种集成电路。这种集成电路简称E/D MOS。 特点 E/D MOS电路的速度快，电压摆幅大,集成密度高。MOS反相器的每级门延迟取决于负载电容的充电和放电速度。在负载电容一定的条件下，充电电流的大小是决定反相器延迟的关键因素。各种MOS反相器的负载特性见图2。在E/D MOS反相器中，作为负载的耗尽型管一般工作在共栅源（栅与源相连,其电压uGS＝0）状态。把耗尽型MOS晶体管的输出特性IDS～VDS曲线，沿纵轴翻转180o,取出其中uGS＝0的曲线,即可得到E/D MOS反相器的负载（图2）。E/D MOS反相器具有接近于理想恒流源的负载特性。与E/E MOS反相器(负载管和驱动管都用增强型MOS晶体管的)相比,同样尺寸的理想E/D MOS电路，可以获得更高的工作速度,其门延迟(tpd)可减少至十几分之一。由于耗尽型管存在衬偏调制效应,E/D MOS反相器的负载特性变差,tpd的实际改进只有1/5～1/8。此外，由于E/DMOS反相器输出电压uo没有阈电压损失，最高输出电压uo可达到电源电压UDD=5伏(图1)。因此，比饱和负载E/E MOS反相器的电压摆幅大。另一方面,由于E/D MOS反相器的负载特性较好，为了达到同样的门延迟,E/D MOS反相器的负载管可以选用较小的宽长比,从而占用较少的面积;为了得到相同的低电平,E/D MOS反相器的βR值也比E/E MOS反相器的βR值小些。与E/E MOS电路相比，E/D MOS电路的集成密度约可提高一倍。 结构与工艺 只有合理的版图设计和采用先进的工艺技术,才能真正实现E/D MOS电路的优点。图3是E/D MOS反相器的剖面示意图。E/DMOS电路的基本工艺与 NMOS电路类同（见N沟道金属-氧化物-半导体集成电路）。其中耗尽管的初始沟道，是通过砷或磷的离子注入而形成的。为了使负载管的栅与源短接，在生长多晶硅之前，需要进行一次"埋孔"光刻。先进的 E/D MOS的结构和工艺有以下特点。①准等平面：引用氮化硅层实现选择性氧化，降低了场氧化层的台阶；②N沟道器件:电子迁移率约为空穴迁移率的三倍,因而N沟道器件有利于提高导电因子；③硅栅自对准：用多晶硅作栅，可多一层布线。结合自对准，可使栅、源和栅、漏寄生电容大大减小。 采用准等平面、 N沟道硅栅自对准技术制作的 E/D MOS电路，已达到tpd≈4纳秒，功耗Pd≈1毫瓦,集成密度约为300门／毫米2。E/D MOS电路和CMOS电路是MOS大规模集成电路中比较好的电路形式。CMOS电路（见互补金属-氧化物-半导体集成电路）比E/D MOS电路的功耗约低两个数量级,而E/D MOS电路的集成密度却比CMOS电路约高一倍,其工艺也比CMOS电路简单。E/D MOS电路和CMOS电路技术相结合，是超大规模集成电路技术发展的主要方向。
