1)Homologous recombination同源重组
1.Construction of cDNA library from mouse oocyte for yeast-2-hybrid system by homologous recombination;用同源重组法构建小鼠卵母细胞cDNA酵母双杂交文库的探索
2.Construction of CHO high expression cell line by means of homologous recombination;利用同源重组建立CHO高表达细胞株
3.Construction of recombinant adeno-viral plasmid bearing hSDF-1α cDNA by homologous recombination in bac-teria and preparation of recombinant adenovirus expressing hSDF-1α;细菌内同源重组快速构建和制备表达hSDF-1α的重组腺病毒
英文短句/例句
1.High efficient generation of recombinant adenovirus by bacterial intermolecular homologous recombination细菌内同源重组法快速制备重组腺病毒
2.The Construction of the Human NGF β Recombinant Adenovirus Vector;细菌内同源重组构建Ad-hNGFβ腺病毒质粒
3.Construction of Recombinant Adenovirus Containing Fusion Suicide Gene CDglyTK by a Simple Method of Homologous Recombination in Bacteria;细菌内同源重组法构建含融合自杀基因CDglyTK的重组腺病毒
4.Construction of the Homologous Recombination Plasmid of sigB in Staphylococcus Aureus;金黄色葡萄球菌sigB同源重组质粒的构建
5.Study of the Homologous Recombination Methods and Inducible Expression System in Z. Mobilis;Z. mobilis中同源重组方法及诱导表达体系的研究
6.Integration and Expression of Δ5 Des Gene in Synechocystis sp.PCC 6803 by Homologous Recombination用同源重组法将Δ5 Des整合在集胞藻6803中的表达
7.( Homologous recombination would presumably be preferred in the case of more slowly-renewing tissues, whose stem cells should not need reseeding.（更新较缓慢的组织中，似应首选同源重组，因其干细胞不需要种植。
8.Cloning of Genomic Islands in Klebsiella Pneumoniae by Using Yeast-Based Homologous Recombination System利用酵母同源重组系统克隆肺炎克雷伯菌基因组岛
9.The Synergistic Activity and Homologous Recombination between CrylAa and CrylC from Bacillus Thuringiensis;苏云金芽孢杆菌CrylAa、CrylC的协同作用和同源重组的研究
10.Application of Homologous Recombination Techniques on the Construction of Expression Vectors in Saccharomyces Cerevisiae;同源重组技术在酿酒酵母表达载体构建中的应用
11.Research on Construction of BAC Library of Guanzhong Milk Goat and Homologous Recombination with Red System;关中奶山羊BAC文库构建及其利用Red系统同源重组的研究
12.Secrete-Expression of Antimicrobial Peptide Bactenecin7 in Lactococcus Lactis and the Construction of Its Homologous Recombination Vector抗菌肽Bactenecin7在乳酸乳球菌中的分泌表达及其同源重组载体的构建
13.Construction of Bacillus subtilis CAS15 dhbC-and dhbC+ Mutants by Using the Homologous Recombination Method同源重组法构建枯草芽孢杆菌dhbC基因缺失突变株和回复株
14.Construction of a Pseudomonas Aeruginosa Strain Bearing a Novel mucA Mutant through Homologous Recombination同源重组构建铜绿假单胞菌新的mucA基因突变菌株
15.Construction of MSTN Knock-out Porcine Fetal Fibroblast同源重组敲除MSTN基因的猪胎儿成纤维细胞的构建
16.Knockout of the Crystalline Inclusion Protein Gene cipA and cipB in Photorhabdus luminescens by RED Homologous RecombinationRED同源重组法敲除发光光状杆菌晶体蛋白基因cipA和cipB
17.Construction of Bacillus subtilis Riboflavin Operon Mutant by Homologous Recombination同源重组法构建枯草芽孢杆菌核黄素操纵子突变株
18.V(D)J recombination and DNA non-homologous end joiningV(D)J重组与DNA非同源末端连接
相关短句/例句
homogenous recombination同源重组
1.Then pDC316-BMP-7 and the framework plasmid pBHGlox_E1,3Cre were transfected into HEK293 cells for homogenous recombination in cells with Lipofectamine 2000.方法用基因工程技术将人BMP-7基因cDNA亚克隆至穿梭质粒pDC316上,利用脂质体介导的方法将AdMax腺病毒包装系统的骨架质粒pBHGlox_E1,3Cre和穿梭质粒pDC316-BMP-7转染入HEK293细胞,进行同源重组,得到腺病毒重组质粒Ad5-BMP-7,并在其中包装扩增病毒。
2.Methods The human HIF-1α gene fragment was cloned into the shuttle plasmid pAdTrack-CMV to form the transfer vector by the method of homogenous recombination in bacteria, and then was transfected into HEK293 cells with Lipofectamine TM 2000.方法采用基因工程技术,经过亚克隆将人HIF-1α基因片段克隆至穿梭质粒pAdTrack-CMV上,利用pAdEasy系统进行细菌内同源重组后,经脂质体转染HEK293细胞,进行重组腺病毒的包装、扩增。
3.Methods The human p16 gene fragment was cloned into the shuttle plasmid pAdTrack-CMV to form the transfer vector by the method of homogenous recombination in bacteria.方法用基因工程技术将人p16基因cDNA亚克隆至穿梭质粒pAdTrack-CMV上,利用PAdEasy系统进行细菌内同源重组,然后通过脂质体将正确重组体包裹并转染293T细胞,并在其中包装扩增病毒。
3)homologous recombinant同源重组
4)DNA homologous recombinationDNA同源重组
5)Red recombinationRed同源重组
1.The Escherichia coli strain SQ88 was selected as the target strain,to which five of rRNA operons(rrnA,rrnD,rrnE,rrnG and rrnH)were sequentially inactivated using λ Red recombination system.以1株染色体上的5个rRNA操纵元(rrnA、rrnD、rrnE、rrnG和rrnH)被敲除的大肠杆菌(Escherichia coli)菌株SQ88为出发菌,运用λRed同源重组系统和卡那霉素抗性基因筛选重组子,并利用抗性基因两端的FRT位点通过位点专一性重组将抗性基因去除,最终成功构建了1株仅具有单拷贝rRNA操纵元(rrnB)的E。
6)λ Red recombination systemλRed同源重组
延伸阅读
同源重组技术分子式：CAS号：性质：见基因打靶
