1)PCR sequencingPCR测序
1.The DNA electrophoretic bands of PCR products were recovered and purified , then sequenced by PCR sequencing method with one of the two primers.用特异性引物对肌球蛋白轻链 2启动子 (myosinlightchain 2 ,MLC2 ) 糜酶融合基因的转基因新生鼠鼠尾DNA进行PCR筛选 ,低熔点琼脂糖凝胶电泳回收阳性样品PCR所扩出的DNA条带 ,纯化后用同一对引物中的一个进行单引物PCR测序 ,与所转外源基因序列比较 ,进一步确定整合有外源基因的阳性鼠 。
2.The polymorphisms of POU1F1 gene were analyzed by PCR sequencing technique in two goat populations(Xiao xiang yang,Guizhou Black goat) and the effects of genotypes of POU1F1 gene loci on slaghter traits(naked body weight,dressing percentage,rib eye area,waist muscle thickness, net meat weight,net meat percentage,bone weight,flesh and blood rat) were evaluated.本研究采用PCR测序技术分析了POU1F1基因内含子4、外显子6在小香羊和贵州黑山羊2个山羊群体中的多态性及其与屠宰性状的相关性,为中国山羊产肉性能的高效选育和山羊品种分子标记数据库的建立提供分子遗传学依据。
3.Based on the study of physiological-biochemical characters as well as hydrophobic capability and degradation capability,the sequence of 16SrDNA and nutrient balance of hydrophobic hydrocarbon degrading bacteria named HDB-1were analyzed,experimental results showed that PCR sequencing was consistent with cell classification,and hydrophobicities of HDB-1 belong to Pseudomonas.实验表明,PCR测序与细胞分类法得到的结果一致,菌株HDB-1属于假单胞菌属细菌(Pseudomonas),氮、磷则是影响HDB-1菌降解石油的限制因素。
英文短句/例句
1.PCR sequence analysis of the 3 UTR of the SON gene of human and mammalian;人和动物SON基因3’非编码区的PCR测序分析
2.PCR Sequencing and Nutrient Balance of Hydrophobic Hydrocarbon Degrading Bacteria疏水性石油烃降解菌的PCR测序及营养平衡研究
3.An Optimized Protocol for Sequencing EST-PCR Products in Eucalyptus桉树EST-PCR产物测序方案的优化
4.PCR Detection and in Situ PCR Location of Specific DNA from Sugarcane Parental Species;甘蔗亲本种特异DNA序列的PCR检测及原位PCR定位的研究
5.Detection of the genomic sequences of two alphaviruses by one-step RT-PCR两种甲病毒基因组序列的一步RT-PCR检测
6.Application of PCR-pyrosequencing technology for food poisoning of SalmonellaPCR-焦磷酸测序技术在沙门菌食物中毒中的应用
7.Detection of the Bean common mosaic virus with RT-PCR and analysis of the sequences of 3′-terminal region菜豆普通花叶病毒RT-PCR检测及3′末端序列分析
8.Optimization of Pre-enrichment Protocol of PCR-based Method for Detection of Salmonella in Foods应用PCR检测食品中沙门氏菌前增菌程序的优化
9.Direct sequencing of PCR amplification products of Hepatitis B virus complete genome乙型肝炎病毒全基因组PCR扩增产物的直接测序
10.Detection and Sequence Analysis on PCR of Arnebia euehroma Jonst Indueed by Agrobacterium rhizogenes发根农杆菌转化新疆紫草的PCR检测及序列分析
11.Cloning and sequence analysis of partial c-ski gene of Wistar rat,and development of SYBR Green-based real-time fluorescence quantitative PCRWistar大鼠c-ski基因部分序列的克隆、测序及实时荧光定量PCR的建立
12.Detect Parkin Gene Dosage Mutation by Real Time Quantitive PCR and DNA Direct Sequencing;应用实时荧光定量PCR技术结合DNA直接测序检测parkin基因突变
13.Detection of the Mutations of PINK1 Gene Using Real-time PCR and Direct Sequencing;应用实时荧光定量PCR技术结合DNA直接测序技术检测PINK1基因突变
14.Screening for DJ-1 Gene Mutation by Real-time PCR and Direct Sequencing;应用实时荧光定量PCR技术结合DNA直接测序技术检测DJ-1基因突变
15.Development of a Fluorogenic Quantitative PCR Technique for Quantification of HCLV and Genomic Sequence Determination and Analysis of NDV HB92 Strain;建立HCLV荧光定量PCR检测技术和NDV HB92株基因组序列测定及分析
16.Detection of Dengue Virus Using RT-PCR with Tagged Primers in Combination with Sanger Sequencing两种标签引物RT-PCR结合Sanger测序检测登革病毒的研究
17.EGFR Mutations Detection in Non-small Cell Lung Cancer Tissues by Real-time PCR and DNA Sequencing实时荧光定量PCR和测序法检测非小细胞肺癌EGFR基因突变的比较
18.Rapid discrimination of three species of marine Vibrios by using linear-after-the-exponential PCR coupled with pyrosequencing利用线性指数PCR-焦磷酸测序技术快速检测3种海洋弧菌
相关短句/例句
PCR-sequencingPCR-测序
3)PCR-DNA direct sequencingPCR-DNA 测序
4)PCR direct sequencing(PCR-DS)PCR直接测序
5)cloning sequence analysisRTPCR序列测定
6)Sequence analysis with clone of PCR productsPCR产物克隆测序
延伸阅读
DNA测序分子式：CAS号：性质：确定一段DNA中碱基排列的次序。一般用双脱氧测序法，另外还有一种马克西姆-吉尔伯特法，是依据四个碱基对不同试剂，如哌啶及肼的不同的反应能力实现的。可利用克隆载体上接头的两侧引入已知序列(例如Sp6，T7，T3的启动子序列)，当插入目的片段后，即可利用此已知序列的寡核苷酸作引物。常用于未知序列DNA片段的测序。
