单细胞PCR,Single cell PCR
1)Single cell PCR单细胞PCR
1.The single cell PCR method, which has been developed recently, has many advantages in HAB studies, especially for those species which are difficult to culture in the laboratory.其中,单细胞PCR方法是对难以室内培养的有害赤潮藻种进行遗传特征研究的一项重要技术。
英文短句/例句

1.Comparison of Different Lysis Methods in the Single Cell Analysis of the Efficiency and Allele Dropout Rate of Polymerase Chain Reaction for Preimplantation Genetic Diagnosis;不同的DNA制备方法对单细胞PCR扩增率和ADO率影响的比较研究
2.Diagnosing X-linked Ichthyosis by Monoplast Single-round Duplex PCR单细胞单轮二重PCR诊断X-连锁鱼鳞病
3.Genotyping analysis of multiple loci in single cell by primer extension preamplification and degenerate oligonucleotide primed-PCR;PEP、DOP-PCR单细胞多基因位点检测研究
4.Development of PCR and PCR-ELISA Methods for Listeria Monocytogenes Detection;单核细胞增多性李斯特菌PCR和PCR-ELISA检测方法的建立
5.The Study on the Detection of Listeria Monocytogenes by LAMP and PCR;LAMP和PCR检测单核细胞增生性李斯特氏菌的研究
6.Clinical Application of FQ-PCR in Detecting of HCV RNA in Serum and Peripheral Blood Monocular Cells;应用实时荧光定量PCR检测外周血清及单核细胞中HCVRNA含量
7.Triplex PCR Identification and Molecular Typing of Listeria Monocytogenes;单核细胞增多性李斯特菌的三重PCR鉴定与分子分型技术研究
8.Research on Radiation Carcinogenesis Related Genes with Single Cell RT-PCR and Suppressive Subtractive Hybridization;应用单细胞逆转录PCR和抑制差减杂交技术研究辐射致癌相关基因
9.Development of a Rapid Polymerase Chain Reaction Method for Detection of Listeria Monocytogenes in Food;食品中单核细胞增生性李斯特氏菌PCR快速检测方法的建立
10.Comparsion of Nested Polymerase Chain Reaction and Primer Extension Preamplification for Single-cell Sexing;两种PCR方法对单细胞进行SRY基因诊断的对比研究
11.Utilizing Duplex Nested Polymerase Chain Reaction Assay and Multiple Displacement Amplification for Sex-linked Hereditary Desease on Single Cell;运用单细胞双重巢式PCR和MDA技术方法行性连锁遗传病诊断
12.Detection of Listeria Monocytogenes in Food by Fluorescence PCR;应用荧光PCR方法检测食品中的单核细胞增生李斯特氏菌
13.Study on Real-time PCR for the Detection of Listeria Monocytogenes in Meat荧光定量PCR检测猪肉中单核细胞增生性李斯特氏菌的研究
14.Detection of FOXP3 mRNA expression in human peripheral blood mononuclear cells by real-time fluorescent quantitative RT-PCR实时荧光定量RT-PCR检测人外周血单个核细胞中FOXP3 mRNA的表达
15.Application of Polymerase Chain Reaction on Rapid Detection of Listeria monocytogenes in FoodPCR在快速检测食品单核细胞增多性李斯特菌中的应用
16.Expression of siglec-1 mRNA in monocytes from patients with primary biliary cirrhosis assayed by FQ-PCR实时定量PCR检测原发性胆汁性肝硬化单核细胞中Siglec-1 mRNA的表达
17.Studies on specific detection of Listeria monocytogenes in foods by duplex PCR食品中单核细胞增生李斯特菌特异性二重PCR检测方法的研究
18.Improved Flinders Technology Associates (FTA) Filter Paper-based DNA Extraction with Polymerase Chain Reaction (PCR) for Rapid Detection of Listeria monocytogenes改良FTA-PCR快速检测单核细胞增生李斯特氏菌研究
相关短句/例句

single-cell RT-PCR单细胞RT-PCR
1.Methods: Human neutrophils were isolated and purified from volunteers,total RNA was extracted and a regular RT-PCR aiming at IL-8Rβ mRNA was performed to ascertain its expression profile in human neutrophils and optimize the reaction conditions for the following single-cell RT-PCR procedures.方法:采用倍比稀释相对定量单细胞RT-PCR方法分离纯化人外周血中性粒细胞并提取总RNA,针对IL-8RβmRNA设计引物,进行RT-PCR确认IL-8Rβ在中性粒细胞中的基因表达并优化反应条件。
3)single cell fluorescent polymerase chain reaction单细胞荧光PCR
1.Objective To explore the feasibility of using single cell fluorescent polymerase chain reaction(PCR)in the preimplantation genetic diagnosis(PGD).结论利用单细胞荧光PCR技术进行着床前遗传学诊断是可行的。
4)Single cell nested RT-PCR单细胞巢式RT-PCR
5)preimplantation genetic diagnosis (PGD)单细胞巢式PCR
6)monoblast single-round duplex PCR单细胞单轮二重PCR
1.To some extent,the protocol of monoblast single-round duplex PCR for diagnosing XLI is accurate,specific and reliable.结果表明单细胞单轮二重PCR诊断XLI具有较高的准确性和特异性,有助于我们进一步开展XLI的PGD。
延伸阅读

单细胞克隆技术分子式:CAS号:性质:平板培养是为了分离和获得单细胞无性系并对不同的无性系进行研究,揭示它们在生理、生化和遗传上的差异而设计的一种单细胞培养技术,也称单细胞克隆技术。由于它具有筛选效率高、筛选量大、操作简单便等优点,因而被广泛地用于遗传变异、细胞分裂分化、细胞次生代谢物合成等项研究。这项技术是1960年Bergmann首创的,操作时将单细胞悬浮液与30~35℃呈融化状态的琼脂均匀混合,然后注入培养皿中成一薄层,琼脂冷却固化后,均匀分布的细胞就在其中成长,由于薄薄的一层固化琼脂呈平板状,故称它为平板培养。