定点饱和突变,Site-saturation mutagenesis
1)Site-saturation mutagenesis定点饱和突变
2)saturation site-directed mutagenesis饱和定点突变
1.In order to further improve its catalytic capability,two mutant libraries,created by saturation site-directed mutagenesis at the amino acid positions 168 and 435,were screened by using the spectroscopic assay based on indigo at 670 nm.利用饱和定点突变技术对细胞色素P450 BM-3(A74G/F87V/L188Q)(PT)的168和435氨基酸位点分别进行随机突变,根据其羟基化吲哚生成的靛蓝在670nm处具有特殊吸收峰进行高通量筛选,获得了三个高于亲本酶(PT)的突变酶D168H、D168L和E435T。
3)saturation mutagenesis饱和突变
1.Directed evolution of cytochrome P450 BM-3 by saturation mutagenesis;饱和突变定向进化细胞色素P450 BM-3
英文短句/例句

1.Saturation mutagenesis of three amino acid positions consisting of the active site of an endoglucanase from termite Coptotermes formosanus台湾家白蚁内切葡聚糖酶活性中心氨基酸的饱和突变
2.Cloning of the Glyphosate N-acetyltransferase Gene、Characterization of Enzyme Activity and Analysis of the Saturate Mutagenesis at AlbD Enzyme S40 Site;草甘膦N-乙酰转移酶基因的克隆、酶学性质研究及AlbD酶S40位点饱和突变分析
3.The Saturation Identification of Current Transformer Based on the Sudden Changes of Current Waveforms基于波形突变的电流互感器饱和辨识
4.Breeding of Highly Productive PUFAs Mutant Strain of Mortierella Isabellina by Co~(60) Mutating Agent Treated;Co~(60)辐射诱变深黄被孢霉高产多不饱和和脂肪酸突变株的选育
5.Study on the Stability of Slope in Unsaturated Soil Affected by Rainfall Infiltration Based on Catastrophe Theory;考虑降雨入渗影响的非饱和土边坡突变失稳的研究
6.exhaustive mutagenesis彻底诱变,全面诱变,饱和诱变
7.Study on Strength and Deformation of Unsaturated Expansive Soils;非饱和膨胀土强度和变形特性的研究
8.EXPERIMENTAL STUDY ON CHARACTERISTICS OF DEFORMATIONAND STRENGTH OF UNSATURATED CLAY非饱和黏土变形和强度特性试验研究
9.Research on Transformer Differential Protection with New Anti-CT Saturation Principle;新型抗TA饱和变压器差动保护研究
10.Research on the Universality of Unsaturated Soil Strength and Deformation Characteristics非饱和土普适的强度与变形理论研究
11.Robust Control of Markovian Jump Systems with Input SaturationMarkov跳变饱和系统的鲁棒控制
12.The Study on the Metric Suction of Unsaturated Clayey Soils非饱和粘性土基质吸力变化规律研究
13.Research on stress-strain relation of unsaturated cohesive soil非饱和黏性土的应力-应变关系研究
14.The design of testing device magnetorheological fluid saturation intensity磁流变液磁饱和强度测定装置的设计
15.Analytical theory for one-dimensional thermal consolidation of saturated soil under time-dependent loading变荷载下饱和土一维热固结解析理论
16.Test study of creep of the unsaturated Nanning expansive soil非饱和南宁膨胀土蠕变特性试验研究
17.Law of Consolidation Creep Strain and Stress Relaxation in Saturated Soft Clay饱和软粘土固结、蠕变变形和应力松弛规律
18.Spontaneous and induced mutation occur with animals viruses.动物病毒的突变有自发突变和诱发突变。
相关短句/例句

saturation site-directed mutagenesis饱和定点突变
1.In order to further improve its catalytic capability,two mutant libraries,created by saturation site-directed mutagenesis at the amino acid positions 168 and 435,were screened by using the spectroscopic assay based on indigo at 670 nm.利用饱和定点突变技术对细胞色素P450 BM-3(A74G/F87V/L188Q)(PT)的168和435氨基酸位点分别进行随机突变,根据其羟基化吲哚生成的靛蓝在670nm处具有特殊吸收峰进行高通量筛选,获得了三个高于亲本酶(PT)的突变酶D168H、D168L和E435T。
3)saturation mutagenesis饱和突变
1.Directed evolution of cytochrome P450 BM-3 by saturation mutagenesis;饱和突变定向进化细胞色素P450 BM-3
4)site-directed mutagenesis定点突变
1.Site-directed mutagenesis of functional gene fragment producing CTX-M-14-type extended-spectrum β-lactamase;CTX-M-14型超广谱β-内酰胺酶基因的定点突变研究
2.Study on site-directed mutagenesis and kinetics of trehalose synthase from Thermobifida fusca;Thermobifida fusca海藻糖合成酶的定点突变及其动力学性质研究
3.The Expression of hBmp-4 in the Pichia Pastoris and Its Site-Directed Mutagenesis;hBmp-4在甲醇酵母中的表达及其定点突变
5)Site directed mutagenesis定点突变
1.Using recombinant glucagon expression system,the 21st aspartic acid was changed to be alanine by means of site directed mutagenesis.在获得重组胰高血糖素基因工程菌基础上 ,利用定点突变技术改造其第 2 1位氨基酸天冬氨酸为丙氨酸 ,并经DNA测序证明胰高血糖素基因发生了点突变 。
2.Two p 16 mutants, P48L and D74N, were obtained by site directed mutagenesis using two step PCR method.应用PCR体外定点突变技术,构建了p16-P48L和p16-D74N突变体。
3.Human brain derived neurotrophic factor gene was used as template for oligonucleotide based site directed mutagenesis and PCR.应用寡核苷酸诱导的定点突变和PCR技术对人脑源性神经营养因子基因进行突变,并完成了测序鉴定。
6)site-directed mutation定点突变
1.A site-directed mutation at 2753 bp of the ret cDNA induced by PCR;PCR介导的ret基因体外定点突变
2.Four conservative tryptophan sites were selected to actualize site-directed mutation.选取了四个色氨酸保守性位点,分别进行定点突变,构建了CpeS的四个色氨酸突变体,分别为CpeS(W14I)、CpeS(W69M)、CpeS(W75S)和CpeS(G119C,W140V)。
3.OBJECTIVE: To obtain sequence of α—glucosidase, SWISS-MODEL Server predicted that enzyme structure, amino acid property and characteristic, to design molecular of alpha-glucosidase,make two site-directed mutation by TaKaRa MutanBEST Kit,express the mutant gene in E.目的:使用PCR技术获得α—葡萄糖苷酶基因序列,通过SWISS-MODEL服务器对α—葡萄糖苷酶结构进行预测,结合氨基酸的性质特点,对酶蛋白进行分子设计,采用PCR突变试剂盒对其进行定点突变,并在大肠杆菌中表达,测定了重组菌、突变菌的酶活力,为进一步研究α—葡萄糖苷酶结构与功能的关系奠定了基础。
延伸阅读

定点突变法分子式:CAS号:性质:又称定点诱变或定位诱变。采用基因合成技术或取相应的寡核苷酸,用酶法(或化学方法)改变基因中某一点上密码子,再把含有该点突变的基因插入到质粒上,形成重组质粒,经转化把重组质粒引入到酵母菌、大肠杆菌等新宿主细胞中进行表达,其表达的产物是一个突变蛋白或突变酶。采用定点突变的方法,可灵活而巧妙地勾画甚至确定某特定氨基酸残基在蛋白质和酶分子中的功能和作用。也是创建工程化蛋白或酶的工具方法之一。