1)ELISA[英][i'laiz?][美][?'la?z?]酶联法
1.Methods The Toxoplasma gondii IgG and IgM antibody (PTA) of serum samples were assayed by ELISA method.方法采用酶联法(ELISA)检测标本血清中弓形虫的IgG、IgM,初筛检测阳性,复查仍为阳性,判断为阳性。
英文短句/例句
1.A Double-antibody Sandwich Method of Dot-ELISA for the Detection of Rotavirus双抗体夹心斑点酶联法检测轮状病毒的研究
2.Determination of Saliva Lysozyme with Enzyme-linked Rocket Immunoelectrophoresis酶联免疫火箭电泳法测定唾液溶菌酶
3.Experimental study on coupled method for assay of erythrocyte GSH-px酶偶联法测定红细胞谷胱甘肽过氧化物酶
4.Objective:In order to build the method to apply Torpedo Acetylcholinesterase(AChE) labelled second antibody in ELBA.目的:以电鳐乙酰胆碱酯酶标记二抗,建立该酶的酶联免疫吸附方法。
5.Influence of icterus on hexokinase(HK) method for determining urea nitrogen.黄疸对酶偶联法测定尿素氮的影响
6.Determination of Sudan Ⅰ by Indirect Competitive Enzyme Linked Immunosorbent Assay;苏丹红Ⅰ号酶联免疫分析方法的建立
7.Development of Enzyme-Linked Immunosorbent Assay for Ractopamine;莱克多巴胺酶联免疫吸附法（ELISA）的建立
8.Development of Enzyme-linked Immunosorbent Assay for Chlorpromazine氯丙嗪酶联免疫检测方法（ELISA）的建立
9.Enzyme-Linked Immunoassay for the Determination of Glycoprotein from the Edible's Nest燕窝糖蛋白酶联免疫检测方法的研究
10.Analysis of False Positive by Euzymelinked Immunosorbent Assay to Detect HBsAg酶联免疫法检测HBsAg假阳性原因分析
11.Enzyme-linked Immunosorbent Assay for Deltamethrin溴氰菊酯酶联免疫吸附分析方法研究
12.Quality Evaluation of HbsAg ELISA Diagnostical ReagentHBsAg酶联免疫法诊断试剂的质量评价
13.HbsAg DETECTION WITH ELISA AND RESOLVING FIA酶联免疫法与时间分辨荧光免疫法检测HBsAg
14.Determination CEA by Combination of ICA and the ELISA化学发光法与酶联免疫吸附分析法联合测定癌胚抗原的应用
15.Studies on the method of controlling enzymatic reaction time of acetylene reduction in determining associative nitrogenase activity of associative system联合固氮酶活性测定中酶促反应时间的控制方法研究
16.Study on Diagnosis of Neonatal Chlamydia Trachomatis Pneumonia by Ligase Chain Reaction Enzyme Linked Immunoadsorbent Assay小婴儿沙眼衣原体肺炎的连接酶链反应—酶联免疫吸附法诊断研究
17.Study on the Detection of PCR-Amplified DNA of Legionellae by Enzyme-Linked Immunoassay;聚合酶链反应—酶联免疫吸附法检测军团菌的实验研究
18.Establishment and Application of ELISA Method for Detecting of Human Serum Heparanase酶联免疫吸附试验定量检测血清肝素酶方法的建立及应用
相关短句/例句
TRAP ELISATRAP酶联法
3)Enzyme-coupled assay酶偶联法
4)Enzymes combined method酶耦联法
5)ELISA[英][i'laiz?][美][?'la?z?]酶联免疫法
1.Application in Food Clenbuterol Poisoning of ELISA Screening and Confirmation by GC-MS;克仑特罗食物中毒的酶联免疫法筛选与气-质联机确证研究
2.Enzyme Linked Immunosorbent Assay(ELISA) and LC-MS/MS for Determination of Furazolidone Residues in Aquatic Products;酶联免疫法与LC-MS/MS法测定水产品中呋喃唑酮代谢物
3.Methodology Study of Indirect Competitive ELISA for Detection of Vomitoxin;酶联免疫法检测呕吐毒素的方法研究
6)Enzyme-linked immunosorbent assay酶联免疫法
延伸阅读
酶联免疫吸附测定法酶联免疫吸附测定法 诊法。免疫标记技术之 一。用于测定抗精子抗体。原理为将欲测血清与相应的固相抗原形成免疫复合物，由标记上 酶(过氧化氢酶、碱性磷酸酶、尿素酶等)的二抗检测。利用这结合的酶使基质(邻苯二胺、 对硝基苯酚、尿素等)转化为色素原，出现的颜色反应以目测或分光光度计测出。方法：用 PBS洗涤精子，将其密度调至25×106/50μl。取50μl加到微量滴定盘的每一凹孔中， 以025%戍二醛固定。用含005%Tween20(表面活性剂)的PBS(PBS-Tween)洗3次，加入50 μl稀释的血清或精浆(1∶4稀释)，在37℃下孵育60分钟后，用PBS-Tween液将凹孔洗3次， 加入50μl，1∶100稀释的联结有磷性磷酸酶的羊抗人免疫球蛋白，经37℃60分钟孵育，再 用PBS-Tween液洗3次，加入250μl含1mg/ml磷酸对硝基苯酚的005M碳酸钠缓冲液，3 7℃60分钟孵育后，每孔加入50μl3NNaOH以中止反应。用分光光度计在400nm测定吸收光 谱。含磷酸对硝基苯酚和3NNaOH作为空白。本法优点在于不需新鲜精子，适宜大量标本测定 ，但与无关抗原的非特异性反应是主要问题。
