1)Nucleic acid amplification techniques核酸扩增技术
英文短句/例句
1.The Evaluation of Virus Inactivation Effect by Using the Technique of Long-fragment DNA Amplification;长链核酸扩增技术评价病毒灭活效果的研究
2.Research advances in nucleic acid amplification technology to detect Gonococcus核酸扩增技术在淋球菌检测中的研究进展
3.Nucleic Acid Sequence-Based Amplification and Its Applications in Viral DiagnosisNASBA(依赖核酸序列的扩增)技术及其在病毒检测中的应用
4.Effect of HPV Multiplex PCR Fluorescence Test on Cervical Cancer Screening多重核酸扩增荧光检测技术在宫颈癌筛查中的效果评价
5.Magnetism Bead-oligonucleotide Microarrays-based Multiplex Amplifiable Probe Hybridization Technology Establishment and Application in DMD Detection;磁珠—寡核苷酸微阵列多重可扩增探针杂交技术的建立及在杜氏肌营养不良检测中的应用
6.Study on a Loop Mediated Isothermal Amplification (LAMP) for the Detection of Enterobacter Sakazakii in Infant Formula环介导等温核酸扩增（LAMP）技术检测婴儿配方奶粉中阪崎肠杆菌的研究
7.Optimal DNA amplification of lab-on-a-chip for loop-mediated isothermal amplification环介导等温扩增芯片核酸最佳扩增条件研究
8.Nucleic acid amplification test of blood donations in blood bank核酸扩增检测在血液筛查的初步应用
9.Study on PCR Amplification of Nucleic Acid of SARS-COV Structural ProteinSARS-COV结构蛋白N的核酸PCR扩增
10.The Use of the Phage Amplifted Biologically Assay in the Study on Drug Susceptibility Testing of Mycobacterium tuberculosis噬菌体生物扩增技术在结核分枝杆菌药敏检测中的应用
11.A convenient and fast method to amplify DNA fragments from filamentous fungi丝状真菌脱氧核糖核酸片段快速扩增法
12.Comparison of three nucleic acid amplification assays for clinical Japanese encephalitis specimens三种核酸扩增方法检测临床乙脑标本的比较
13.Restraint Mechanism of Technology Diffusion under the Visual Angle of Growth of Economy;经济增长视角下技术扩散的约束机制
14.International Technology Diffusion and Economic Growth:A Growth Rate Convergence Model;国际技术扩散与经济增长:收敛模型
15.APPLICATION OF STR MULTIPLEX AMPLIFYING SYSTEM WITH 12 STR LOCI IN PATERNITY TESTING;STR复合扩增技术在亲子鉴定中的应用
16.Loop mediated isothermal amplification(LAMP) method for detection of Vibrio cholerae霍乱弧菌环介导等温扩增LAMP技术检测
17.Bibliometric Analysis of Loop-mediated Isothermal Amplification环媒恒温基因扩增技术文献计量分析
18.Technology Diffusion and Regional Economic Growth in Shandong--An Empirical Analysis Based on Model of Technology Diffusion技术扩散与山东区域经济增长——基于技术扩散模型的一个实证分析
相关短句/例句
isothermal DNA amplification体外核酸扩增技术
3)polymerase chain reaction核酸体外扩增技术
4)nucleic acid sequence-based amplification基于核酸序列的扩增技术
1.The study was purposed to investigate diagnostic value of late-mRNA detection by nucleic acid sequence-based amplification ( NASBA) technique for human cytomegalovirus (HCMV) infection of the recipients after allo-geneic peripheral blood stem cell transplantation (allo-PBSCT) and to evaluate the clinical significance for guiding antiviral therapy.本研究探讨基于核酸序列的扩增技术(nucleicacidsequencebasedamplification,NASBA)检测mRNA对异基因外周血干细胞移植受者术后人类巨细胞病毒(HCMV)感染的诊断价值,并评价该技术对抗病毒治疗的指导意义。
5)Reverse Transcription Loop-mediated Isothermal Amplification逆转录环介导等温核酸扩增技术
1.Evaluation of Reverse Transcription Loop-mediated Isothermal Amplification for Detection of Avian Influenza A H5N1 Virus;逆转录环介导等温核酸扩增技术(RT-LAMP)在H5N1禽流感病毒基因检测中的应用
6)NASBA依赖核酸序列的扩增技术
延伸阅读
核酸体外扩增技术分子式：CAS号：性质：又称基因体外扩增技术或核酸体外扩增技术。利用两种与相反链杂交并利用附着于目标DNA两端的寡核苷酸引物在高温(95℃)使含目标DNA片段的DNA双链变性，分解为单链。在较低温度(37～63℃)与引物退火：然后变温到72℃左右，在耐高温DNA聚合物酶作用下引物被沿样板DNA单链延伸合成互补链，然后又变性→退火→延伸反复进行。引物大大过量，dNTP过量，酶在高温中是较稳定的，这样经过几十个循环，目标DNA片段就按2n数递增。用此法可从mRNA中，cDNA库中扩增出目标基因。过去一般合成500～1000bp较好，现已发展出大片段(75kb)的PCR，且差错甚少。PCR技术的发展还可用于定点突变，质粒重组及FLP等方面。这一技术的作用范围日益扩大，已成为分子生物学工作者基本技术之一。这项专利技术1985年由美国塞特斯(Cetus)公司开发。是20世纪80年代分子生物学领域中的一项革命性突破，已在分子生物学、医学、法学等领域发挥了极大的作用。
