1)ISSRISSR反应体系
1.To optimize ISSR amplification system on Zantedeschia,the effect of four factors, Mg~(2+),Taq DNA Polymerase,primer and DNA,were studied.通过不同浓度Mg~(2+)与TaqDNA聚合酶;模板DNA与引物的组合试验,确定马蹄莲属植物最佳ISSR反应体系为:每20μl中含1×PCR buffer、2。
英文短句/例句
1.Optimization of ISSR reaction system and its preliminary application in wolfberry(Lycium barbarum L.)枸杞ISSR反应体系的优化与应用
2.Establishment and Optimization of ISSR Reaction System for Apis cerana东方蜜蜂ISSR反应体系的建立及优化
3.Establishment and Analysis of ISSR Reaction System for Sisal剑麻ISSR反应体系的建立与分析
4.Establishment and optimization for ISSR-PCR reaction system in poinsettia一品红ISSR反应体系的建立与优化
5.Establishment and Optimization of ISSR Reaction System in Cauliflower花椰菜最适ISSR反应体系的建立及优化
6.Study on Optimization for ISSR Reaction System of Asparagus cochinchinensis(Lour.) Merr.with Orthogonal Design正交优化设计天冬ISSR反应体系的研究
7.Establishment of ISSR-PCR Reaction System for Swida wilsoniana光皮树无性系ISSR-PCR反应体系的建立
8.Optimization for ISSR Reaction System of jujube tree by Orthogonal Design正交设计优化枣树ISSR-PCR反应体系
9.Optimization of ISSR-PCR Reaction System in Wampee黄皮ISSR-PCR反应体系的优化
10.Optimization for ISSR-PCR Reaction Conditions of Ornamental Peach观赏桃ISSR-PCR反应体系的优化
11.Establishment of ISSR-PCR Reaction System in the Ottelia acuminata(Gagnep.) Dand海菜花ISSR-PCR反应体系的优化
12.Establishment and optimization of ISSR-PCR reaction system of Nelumbo nucifera Gaertn荷花ISSR-PCR反应体系的建立及优化
13.Optimization of ISSR-PCR System of Sweet Potato甘薯ISSR-PCR反应体系的优化
14.Optimization of ISSR-PCR Reaction System in Yane geese雁鹅ISSR-PCR反应体系的优化
15.Optimization of ISSR-PCR Reaction System for Medicinal Plant Gentiana Crassicaulis Duthie Ex Burk粗茎秦艽ISSR-PCR反应体系的优化
16.Optimization of ISSR-PCR Reaction System for Miscanthus floridulus五节芒ISSR-PCR反应体系的优化
17.Optimization for ISSR Reaction System of Monopterus albus with Orthogonal Design黄鳝ISSR-PCR反应体系的建立及条件优化
18.Establishment and Optimization of ISSR-PCR Reaction System for Picea crassifolia kom.青海云杉ISSR-PCR反应体系的建立与优化
相关短句/例句
ISSR reaction systemISSR反应体系
1.The results showed that optimum ISSR reaction system of calla lily was every 20 μL reaction system containing 1×PCR Buffer, 1.选取白花马蹄莲和10个颜色有代表性的彩色马蹄莲栽培品种为材料,采用改进的SDS法提取叶片基因组DNA,通过正交设计研究Mg2+、Taq DNA聚合酶和模板DNA、引物的影响,确定彩色马蹄莲最佳ISSR反应体系为:每20μL中含1×PCR Buffer、1。
2.Results A suitable ISSR reaction system was constructed with the 20 μL reaction system containing 1.所建立的药用菊花ISSR反应体系具有标记位点清晰、反应系统稳定、检测多态性能力较强、重复性好等特点,可用于药用菊花的遗传多样性研究。
3)ISSR-PCR reaction systemISSR-PCR反应体系
1.In order to establish a stable ISSR-PCR reaction system of Scrophularian ningpoensis Hemsl,some compatible PCR systems were screened by orthogonal design on 5 factors(dNTPs,Taq DNA polymerase,MgCl2,primer and DNA template) and 5 levels,meanwhile the factors and reaction procedure(annealing temperature,elongate time and cyclic numbers) were optimized by single factor gradient.为了建立稳定的玄参ISSR-PCR反应体系,本试验采用5因素(dNTPs、Taq DNA聚合酶、MgCl2、引物及模板)5水平正交设计筛选PCR体系,并利用单因子梯度进一步优化反应体系各因素浓度和扩增程序(退火温度、延伸时间和循环次数)。
2.[Objective] The study aimed to establish the effective and stable ISSR-PCR reaction system for Manglietia grandis Hu et Cheng.[目的]建立高效稳定的大果木莲ISSR-PCR反应体系。
4)ISSR-PCR reactionISSR-PCR反应
1.The most suitable ISSR-PCR reaction system and amplified procedure for C.以改良的CTAB法提取的寒兰(Cymbidium kanran Makino)基因组DNA为模板,通过单因子试验建立最适的寒兰的ISSR-PCR反应体系。
5)ISSR-PCR systemISSR-PCR体系
1.This paper analyzed the influence of the 6 factors on the reaction results,the factors included:the concentration of Mg2+,dNTP and BSA in ISSR-PCR system,as well as the dosage of the template DNA,primer and Taq DNA polymerase.分析了ISSR-PCR体系中Mg2+、dNTP和BSA浓度,以及模板DNA、引物和TaqDNA聚合酶用量等6个因素对反应结果的影响,建立了适合于台湾水青冈ISSR-PCR扩增的反应体系:10μL PCR反应液中,含10 ng DNA,1。
6)ISSR Rreaction System OptimizationISSR体系优化
延伸阅读
反应素性变态反应反应素性变态反应 见"I型变态反应"。
