聚合酶链反应-序列特异引物,PCR-SSP
PCR-SSP聚合酶链反应-序列特异性引物
1.Methods A total of 192 RhD positive samples,and 166 RhD negative samples were tested by PCR-SSP for exons of RHC/E and D genes.方法用聚合酶链反应-序列特异性引物(PCR-SSP)扩增技术,扩增Rh血型C/E基因,D基因的外显子,检测192例RhD阳性个体,166例RhD阴性个体的血样品。
2.Methods:HLA-DRB alleles of 82 vitiligo patients and 80 psoriasis vulgaris patients were detected by polymerase chain reaction sequence-specific primer(PCR-SSP),under the comparison with 86 healthy persons.方法:采用聚合酶链反应-序列特异性引物(PCR-SSP)分型技术,对82例汉族白癜风患者和80例寻常型银屑病患者HLA-DRB1等位基因进行检测,与86名汉族健康人群进行对照。
3.Methods A total of 88 umbilical cord blood samples,including those randomly selected from Tianjin stem cell bank and already matched for transplantation,were performed polymerase chain reaction sequence specific primers(PCR-SSP) and sequencing based type (SBT) to check their type results and sequencing based type.方法采用聚合酶链反应-序列特异性引物扩增(PCR-SSP)方法、直接测序分型穴SBT雪方法,随机抽查天津市脐带血造血干细胞库脐带血及查询已选中做脐带血移植的样本共88例SSOP方法的结果进行HLA基因分型复核。
3)polymerase chain reaction-sequence specific primer聚合酶链反应-序列特异性引物
1.Methods HLA-DR15 was detected by the technique of polymerase chain reaction-sequence specific primers(PCR-SSP) and compared between peripheral blood of MDS group(n=59)and control group(n=104).方法采用聚合酶链反应-序列特异性引物(PCR-SSP)检测MDS患者(n=59)HLA-DR15基因的表达,并与健康对照组(n=104)进行比较。
2.Methods Polymerase chain reaction-sequence specific primer (PCR-SSP) was used to analyze the distribution of HLA-A alleles among 106 patients with systemic lupus erythematosus and 122 healthy persons.方法应用聚合酶链反应-序列特异性引物(PCR-SSP)的方法,对106例SLE患者和122例健康人进行HLA-A等位基因的检测。
4)Polymerase chain reaction-sequence specific primers聚合酶链反应/序列特异性引物
5)PCR-SSP聚合酶链反应序列特异引物
1.Methods HLA-DRB1 allele frequencies in 32 Chinese patients with ALL,33 Chinese patients with CML and 104 healthy Chinese individuals were examined with polymerase chain reaction-specific sequence primers(PCR-SSP)methods.方法用聚合酶链反应序列特异引物(PCRSSP)方法,对32例ALL、33例CML患者和104例健康志愿者的HLADRB1基因进行分型并分析HLADRB1基因的分布。
2.Methods Using high-resolution polymerase chain reaction amplification with sequence specific primers(PCR-SSP),we detected the distribution frequencies of HLA-Cw*0602 allele among 52 patients with PV and 60 healthy controls,and compared witi each other.方法采用聚合酶链反应序列特异引物(PCRSSP)法,检测52例寻常性银屑病患者及60名健康对照者的等位基因频率,并相互比较。
6)polymerase chain reaction-sequence specific primers聚合酶链反应-序列特异性引物
1.Methods The technique of polymerase chain reaction-sequence specific primers (PCR-SSP) was adopted in genotyping a sample of 8962 unrelated healthy individuals collected from a Chinese Han population in Liaoning area.方法用聚合酶链反应-序列特异性引物方法对辽宁8962名健康无关汉族人进行HLA-B等位基因分型,计算HLA-B等位基因频率并与不同人群HLA-B等位基因的多态性进行比较。
延伸阅读

聚合酶链反应聚合酶链反应polymerase chain reaction,PCR 是用特异性寡核苷酸引物在DNA聚合酶的作用下对靶DNA序列进行大量扩增的分子生物学方法。PCR技术中以在短时间内把极其微量的特定DNA(或RNA)片断大量增扩,达到用常规方法就可以检测的水平。临床用来确定一些疾病的病原体,因其假阳性率较高,故应谨慎使用。