反转录-聚合酶链式反应,RT-PCR
1)RT-PCR反转录-聚合酶链式反应
1.Based on the two conserved sequence of the nucleotide sequence of PLRV,a pair of primers were designed and synthesized for each conserved sequence,About 240 bp,400 bp specific PCR fragments were obtained by reverse transcription and polymerase chain reaction(RT-PCR) amplification from extracted RNA,which had the expected length,at the same time.从感染马铃薯卷叶病毒的植株中提取马铃薯卷叶病毒总RNA,针对马铃薯卷叶病毒基因组,其中的2个保守序列分别设计并合成了1对寡聚核苷酸引物,用反转录-聚合酶链式反应方法从提取的病毒RNA材料中扩增出符合设计大小的240 bp、400 bp的特异性产物,对照的健康植株中未扩增出相应产物。
2.Four methods including the virus isolation and identification,fluorescent antibody,reverse transcriptase polymerase chain reaction(RT-PCR) and Sandwich ELISA were used to detect classical swine fever(CSFV) in 23 samples and were compared thereafter.应用病毒分离鉴定、荧光抗体法、反转录-聚合酶链式反应(RT-PCR)和夹心ELISA 4种方法,分别对23份猪瘟病料组织中的猪瘟病毒(CSFV)进行了检测,并比较4种检测方法的优缺点。
3.Examinations of CTV distribution in the hosts different tissues using DTBIA and RT-PCR showed that from the highest frequency of positivity to the lowest were young stem,young leaf,old stem and old leaf.应用直接组织点免疫(DTBIA)和反转录-聚合酶链式反应(RT-PCR),对接种了柑桔衰退病毒(CTV)的3组共45株锦橙、凤凰柚和木并柑进行了检测,结果表明,CTV在嫩枝中的检出率最高,其次依次是嫩叶、老枝和老叶。
2)reverse-transcription polymerase chain reaction反转录-聚合酶链式反应
3)reverse transcription polymerase chain reaction反转录聚合酶链式反应
英文短句/例句

1.Rapid Detection of Tobacco Mosaic Virus from Crude Samples on Oscillatory-flow Reverse Transcription Polymerase Chain Reaction Microfluidics微流控振荡流反转录聚合酶链式反应快速检测烟草花叶病毒
2.Study on Multiplex Reverse Transcription Polymerase Chain Reaction for Detection of H5,H7 and H9 Subtypes Avian Influenza Virus多重反转录聚合酶链式反应检测H5、H7和H9亚型禽流感病毒的研究
3.Development of an Antigen Capture Reverse Transcriptase/Polymerase Chain Reaction for Detection Foot-and-Mouth Disease Virus;抗原捕获反转录聚合酶链式反应(AC-RT/PCR)检测口蹄疫病毒方法的建立
4.Studies on the Detection of Bovine Viral Diarrhea-mucosal Disease Virus Using Reverse Transcription-nested Polymerase Chain Reaction;反转录—套式聚合酶链式反应检测牛病毒性腹泻/粘膜病病毒的研究
5.Studies on the Detection and Differentiation of Virulent and Avirulent Strains of Newcastle Disease Virus in Chickens Using Reverse Transcription-Nested Polymerase Chain Reaction;反转录—套式聚合酶链反应检测、鉴别鸡新城疫病毒强、弱毒株的研究
6.Dection of enterovirus infection by reverse transcription and polymerase chain reaction应用逆转录聚合酶链反应检测肠道病毒感染
7.Detection of Rice Stripe Virus by Reverse Transcription and Polymerase Chain Reaction逆转录聚合酶链式反应快速检测水稻条叶枯病毒
8.DETECTION OF ENTEROVIRUS RNA IN HUMAN BLOOD SPECIMENS BY REVERSETRANSCRIPION POLYMERASE CHAIN REACTION逆转录聚合酶链式反应检测人血标本中肠道病毒核糖核酸
9.Detection of mRNA in kidney of dead rat using fluorescence semi-quantitative RT-PCR;荧光半定量逆转录-聚合酶链式反应检测大鼠死后肾mRNA
10.Establishment of reverse transcription semi-nested polymerase chain reaction to the pathogenic diagnosis of JE patients逆转录-聚合酶链反应在乙型脑炎诊断中的建立
11.PCR Assay for Diagnosis of Streptococcus Suis Serotype 2聚合酶链式反应诊断2型猪链球菌
12.Multiplex Reverse Transcription-nPCR: Application in the Study of Causative Organisms of Viral Encephalitis in Children;多重逆转录套式聚合酶链式反应在儿童病毒性脑炎病原学研究中的应用
13.Rapid identification of subtypes of respiratory syncytial virus by real-time reverse transcription PCR实时逆转录聚合酶链反应快速鉴别呼吸道合胞病毒亚型
14.Research of Real-time Quantitative Reverse-transcriptase Polymerase Chain Reaction (RQ-PCR) to Detect bcr/abl Fusion Gene in Chronic Myeloid Leukemia;实时定量逆转录聚合酶链反应检测慢粒bcr/abl融合基因的研究
15.Expression of the multidrug resistance in human bladder tumors by reverse transcription polymerase chain reaction应用逆转录聚合酶链反应技术检测膀胱癌多项耐药基因的表达
16.The detection of circulating hepatocellular carcinoma cells in peripheral venous blood by reverse transcription polymerase chain reaction and its clinical significance巢式逆转录多聚酶链反应检测循环性肝癌细胞及其临床意义
17.Reverse transcription multiplex-nPCR:application in the study on causative organisms of viral encephalitis in children逆转录多重套式聚合酶链反应在儿童病毒性脑炎病原学研究中的应用
18.DETECTION OF FISH VIRAL HEMORRHAGIC SEPTICEMIA VIRUS (VHSV) BY NESTED REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION (RT-PCR)应用巢式逆转录聚合酶链反应检测鱼类病毒性出血性败血症病毒(VHSV)
相关短句/例句

reverse-transcription polymerase chain reaction反转录-聚合酶链式反应
3)reverse transcription polymerase chain reaction反转录聚合酶链式反应
4)RT-PCR反转录聚合酶链式反应
1.Method The CK20 mRNA was detected by RT-PCR in 80 patients with colorectal cancer and 20 normal controls.方法应用反转录聚合酶链式反应(reverse transcriptase polymerase chain reaction,RT-PCR)检测80例结直肠癌患者和20例健康对照者外周血和淋巴结中CK20 mRNA。
2.In the present study, reverse transcription-polymerase chain reaction (RT-PCR) technique was used to detect the changes in the expression of NMDA receptor subunit mRNAs in the hippocampus of neonatal rats after ketamine treatment.本研究利用反转录聚合酶链式反应(RT-PCR)观察了将氯胺酮给予生后早期大鼠后,海马组织中NMDA受体亚型mR-NA的表达变化。
3.A reverse transcription polymerase chain reaction(RT-PCR) technique was used to detect the presence of FMDV in animal products.根据已发表的口蹄疫病毒(FMDV)基因序列设计了一对引物,扩增FMDV 3D后1/3区段,应用反转录聚合酶链式反应(RT-PCR)技术,从组织培养液中扩增出大小为489 bp的基因片段,建立了FMDV RT-PCR检测方法。
5)reverse transcription-polymerase chain reaction反转录聚合酶链式反应
1.An oscillatory-flow reverse transcription-polymerase chain reaction(RT-PCR) microfluidics based on a capillary was developed for the detection of tobacco mosaic virus(TMV).建立了一种基于毛细管的振荡流反转录聚合酶链式反应(RT-PCR)的微流控装置检测烟草花叶病毒(TMV)的新方法。
6)reverse transcriptase polymerase chain reaction (RT_PCR)反转录_聚合酶链式反应
延伸阅读

聚合酶链式反应分子式:CAS号:性质:又称基因体外扩增技术或核酸体外扩增技术。利用两种与相反链杂交并利用附着于目标DNA两端的寡核苷酸引物在高温(95℃)使含目标DNA片段的DNA双链变性,分解为单链。在较低温度(37~63℃)与引物退火:然后变温到72℃左右,在耐高温DNA聚合物酶作用下引物被沿样板DNA单链延伸合成互补链,然后又变性→退火→延伸反复进行。引物大大过量,dNTP过量,酶在高温中是较稳定的,这样经过几十个循环,目标DNA片段就按2n数递增。用此法可从mRNA中,cDNA库中扩增出目标基因。过去一般合成500~1000bp较好,现已发展出大片段(75kb)的PCR,且差错甚少。PCR技术的发展还可用于定点突变,质粒重组及FLP等方面。这一技术的作用范围日益扩大,已成为分子生物学工作者基本技术之一。这项专利技术1985年由美国塞特斯(Cetus)公司开发。是20世纪80年代分子生物学领域中的一项革命性突破,已在分子生物学、医学、法学等领域发挥了极大的作用。