蛋白质免疫印迹,Western blot
1)Western blot蛋白质免疫印迹
1.METHODS: The amelogenin mRNA expression patterns were determined by the reverse transcription polymerase chain reaction (RT-PCR),and the protein expression was studied with Western blotting.方法:采用反转录多聚酶链式反应(RT-PCR)技术,检测培养细胞中釉原蛋白mRNA的表达,采用蛋白质免疫印迹技术检测培养细胞中釉原蛋白的表达。
英文短句/例句

1.Detection of E-cadherin expressions in lung cancer tissues by Western blot analysis蛋白质免疫印迹技术检测肺癌组织上皮钙粘蛋白的表达
2.Immunocytochemistry and western blotting technology were used to identify the cell phenotype.用蛋白质印迹免疫检测技术和免疫细胞化学技术鉴定细胞表型。
3.immunoreactive protein免疫反应性蛋白,免疫活性蛋白(质)
4.Northwestern blottingNorthwestern印迹(法),RNA-蛋白质印迹(法)
5.southwestern blottingsouthwestern印迹法,DNA-蛋白质印迹法
6.Estimation of postmortem interval by determination of troponin I using Western blot technique in human pectoralis major;免疫印迹检测人胸大肌肌钙蛋白I推断较晚期死后经过时间
7.Comparison of Physical Methods of Protein Extraction in Rabbit Bone Tissue for Western Blot兔骨组织总蛋白的提取和在免疫印迹法中的应用
8.③The changes of BMP2 and osteopontin were detected by immunohistochemistry and Western blotting.③骨形态发生蛋白2与骨桥蛋白检测:采用免疫组织化学染色与蛋白印迹方法。
9.Expression of the genes encoding protein antigens of Rickettesia rickettesii and the analysis of the recombinant protein antigens by immunoblotting立氏立克次体蛋白抗原基因的表达和重组蛋白抗原的免疫印迹分析
10.protoBlot system[商]蛋白印迹系统[Promega公司生产的蛋白质印迹成套试剂]
11.The Study on Tagging Certain Proteins from PC3M Binded to the Specific Peptide Concerning the Metastases of Human Prostatic Cancer by Western Blotting;利用免疫印迹法钓取PC3M细胞中与人前列腺癌高转移相关特异性短肽结合蛋白的研究
12.Preliminary Study on Immuoproteomics of Outer Membrane Proteins of Brucella;布鲁氏菌外膜蛋白免疫蛋白质组学的初步研究
13.Proteomic Analysis to Augmented Immune Responses in Mouse by Prime-and-Boost Immunization Strategy with HBsAg DNA and Protein Vaccine;乙肝表面抗原DNA疫苗初免—蛋白疫苗加强免疫策略增强免疫反应的蛋白质组学分析
14.Protection of Mice with DNA Vaccines Encoding Influenza Virus Matrix Protein流感病毒基质蛋白DNA疫苗的免疫保护作用研究
15.Study on Protein-Imprinted Soft-wet Gel Polymeric Beads;蛋白质印迹软湿凝胶聚合物微球的研究
16.Bio-imprinting of Protein/DNA Macromolecules and Electro-driven Catalysis of Enzyme;蛋白质/核酸的生物印迹及酶的电驱动催化研究
17.Preparation and Selective Permeation of Protein Molecularly Imprinted Membranes蛋白质分子印迹膜的制备和渗透性研究
18.The immunoproteomics identification of proteins with immunogenicity activity in T cell vaccineT细胞疫苗免疫原性蛋白的免疫蛋白组学鉴定
相关短句/例句

Western blotting蛋白质免疫印迹法
3)Western blot蛋白质免疫印迹(Western blot)
4)Western blot蛋白免疫印迹
1.Western blot was used to detect the alterations of Kir6 proteins in different time courses (0, 1, 3 and 5 days after 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) injections) at different structures of brain ( the striatum and substantia nigra) in parkinsonian mice.本研究采用蛋白免疫印迹分析(Western blot)对1-甲基-4-苯基-1,2,3,6-四氢吡啶(MPTP)诱导的PD小鼠模型黑质、纹状体Kir6。
2.Methods The expression of MGMT was tested by immunocytochemistry and Western blot.方法利用免疫细胞化学和蛋白免疫印迹(Western blot)方法,检测X射线照射后CNE细胞株MGMT表达情况。
3.Methods The DNA and specific antibody of human bocavirus from pharyngeal extracts, nasopharyngeal swab and serum were detected using real time PCR and Western blot.方法采用荧光定量PCR和蛋白免疫印迹技术检测患者鼻咽部抽取物、咽拭子和血清标本中的人博卡病毒DNA及特异性抗体,并全面分析感染患儿的各种临床症状、体征及常规实验室检测,同时做远期随访。
5)Western Blot免疫蛋白印迹法
1.Methods Western blot and RT-PCR were employed to investigate the expression of hPR isoforms in ectopic lesion from adenomyosis and normal endometrium from controls.方法运用免疫蛋白印迹法(Western blot)和RT-PCR方法测定子宫腺肌病患者(24例)病灶及其在位内膜及对照组(19例)子宫内膜标本中孕激素受体亚型在蛋白和mRNA水平的表达,计算机图像分析、半定量研究孕激素受体亚型的表达。
6)Western blotting蛋白免疫印迹
1.Objective: To determine the optimum experiment condition of detecting P—glycoprotein with Western blotting.目的:确定蛋白免疫印迹检测大鼠脑组织中P-糖蛋白的最佳实验条件。
延伸阅读

蛋白质印迹法分子式:CAS号:性质:一种免疫测定微量蛋白质的技术。样品经SDS-聚丙烯酰胺凝胶电泳后,将分辨的蛋白质转移到硝酸纤维膜上,加入放射标记的抗体一起保温,进行抗原抗体结合反应。然后冲洗去掉未结合的抗体,将薄膜贴在X线底片上,通过放射自显影进行定位和检测。