RNA干涉,RNA interference
1)RNA interferenceRNA干涉
1.Inhibitory effect on human leukemia K562 cells by RNA interference of stathmin gene;stathmin基因的RNA干涉抑制人白血病K562细胞体外增殖的作用
2.Study of apoptosis of human cervical carcinoma HeLa cellsby RNA interference targeting at VEGF_(165);VEGF_(165) RNA干涉诱导人宫颈癌HeLa细胞凋亡的研究
3.Construction of RNA interference expression plasmid of human neuropathy target esterase by cloning reverse repeats into plasmid DNA;反向重复序列法构建神经病靶标酯酶RNA干涉表达质粒
英文短句/例句

1.Construction of a Series of RNA Interference Vectors and Their Application in the Study of Genes Involved in the Invasion and Metastasis of Hepatocellular Carcinoma;RNA干涉技术平台的建立及肝癌侵袭转移相关基因RNA干涉研究
2.Inhibition of HPV16 E6 Oncogene in Cervical Cancer by RNA Interference;RNA干涉抑制子宫颈癌HPV16 E6基因的研究
3.Construction of Double-stranded RNA Interference Vector Against Potato Leafroll Virus;马铃薯卷叶病毒双链RNA干涉载体构建
4.Establishment and Use of GATEWAY RNA Interference System in Rice;水稻GATEWAY RNA干涉技术的建立及应用研究
5.Study on the RNA Interference of Sry in Mouse Embryos;小鼠胚胎Sry基因的RNA干涉研究
6.Study on Molecular Features of Small Interference RNA;RNA干涉效应分子siRNA分子特性的研究
7.Study of Human Era Function with RNA Interference;用RNA干涉研究人era基因的功能
8.RNAi-mediated Multi-virus Resistance in PotatoRNA干涉介导的马铃薯抗病毒研究
9.RNA Interfrence of SUMO1 Mediated by Dual Promoter Vector基于双启动子载体的SUMO1 RNA干涉实验
10.The Application of RNA Interference in Cotton ResearchRNA干涉及其在棉花研究中的应用
11.Inhibited Proliferation of Human Breast Carcinoma Cells by RNA Interference-based Knockdown of erbB2/HER2;针对HER2的RNA干涉对人乳腺癌细胞生长的影响
12.The Application of RNA Interferential Technology in Tobacco Breeding of Resisting to TMV;RNA干涉技术在烟草抗TMV病毒育种中的应用
13.Agrobacterium Tumefaciens-mediated Transformation by Using BADH2-derived RNAi Construct in Rice (Oryza Sativa);水稻BADH2基因RNA干涉表达载体的构建与遗传转化
14.Construction and Utilization of RNAi Vectors for the Functional Candidate Genes in Rice;水稻功能候选基因RNA干涉载体的构建及其应用
15.Functional Identification of SDKB Gene in Rice Through RNA Interference;应用RNA干涉技术对水稻SDKB基因的功能鉴定
16.Transform and Expression of Exogenous RNA Interferential Gene in Tobacoo外源RNA干涉基因在烟草中的转化及表达
17.Application of RNA interfere technology in research of diabetic nephropathiesRNA干涉技术在糖尿病肾病研究中的应用进展
18.A Preliminary Study on Utilizing RNA Interference to Confer Enhanced Resistance to BmNPV in Transgenic Silkworm利用转基因RNA干涉提高家蚕对BmNPV的抗性初探
相关短句/例句

RNAiRNA干涉
1.Silencing of HER2 Receptor and Growth Inhibition of SKBR3 Breast Cancer Cells by Lentiviral-mediated RNAi;慢病毒介导的RNA干涉对乳腺癌SKBR3细胞HER2受体的下调及生长抑制
2.Construction of RNAi Vectors and their Use in Myrosinase Gene Downreg-ulation in Arabidopsis;植物RNA干涉载体的构建并用于拟南芥芥子酶基因的表达
3.Construction of RNAi expression vector targeting ACO gene in Hosta plantaginea;玉簪ACC氧化酶基因RNA干涉表达载体的构建
3)RNA interference (RNAi)RNA干涉
1.Method According to MSP58 cDNA coding sequence, the specific RNA interference (RNAi) fragments targeting MSP58 gene were designed and synthesized, which were cloned into pSilencer3.目的探讨经载体介导的RNA干涉法建立抑制癌基因微小球蛋白(58-kDa microspherulep rotein,MSP58)表达的稳转细胞系的可行性。
2.Methods According to survivin cDNA coding sequence, the specific RNA interference (RNAi) fragments targeting survivin gene were designed and synthesized, which were cloned into pWH1 plasmid vector, and the shRNA eukaryotic expression vector pWH1-SR targeting survivin gene was const.方法根据SurvivincDNA编码序列,设计并合成针对Survivin基因的特异性RNA干涉片断,将其克隆入pWH1质粒载体,构建Survivin基因shRNA真核表达载体pWH1SR。
3.RNA interference (RNAi), which could silence specific gene expression post-transcriptionally, has become a powerful tool for identifying gene function in eukaryotic cells.在真核细胞基因功能研究中,RNA干涉(RNAi) 已成为一种强有力的选择性沉默基因表达的实验工具。
4)RNA interference(RNAi)RNA干涉
1.RNA interference(RNAi) is first discovered in C.RNA干涉 (RNAinterference ,RNAi)是 1998年首次在秀丽线虫中发现并证明属于转录后水平的基因沉默 (PT GS)机制。
2.An RNA interference(RNAi) pBIN vector of the LEAFY gene was constructed by Gateway technology,a.基于LEAFY基因在植物生长发育中的特性,利用PCR和Gateway技术构建了含有LEAFY基因保守序列的RNA干涉植物表达载体LFY-RI,利用根瘤农杆菌Agrobacterium tumefaciems介导法获得转基因植株,利用RNA干涉技术抑制转基因植株中LEAFY基因的表达,从而获得枫香Liquidambar formosana不育转基因植株。
3.RNA interference(RNAi) is a process in cells to degrade specific homologous mRNA by forming duplex RNA and has been developed into a powerful tool to study gene functions.RNA干涉是指通过形成局部双链RNA进而特异性地降解细胞内同源基因mRNA的机制,目前已成为基因功能研究的有力工具。
5)siRNA小干涉RNA
1.Objective To explore the relationship between specific silencing effect of siRNA that targets latent membrane protein 1(LMP1) coding gene on the proliferation and apoptosis of EBV positive gastric epithelium cells.目的探讨化学合成小干涉RNA(siRNA)对Epstein-Barr病毒(EBV)潜伏膜蛋白1(LMP1)阳性胃上皮(GT38)细胞LMP1编码基因表达的抑制作用,以及对细胞增殖和凋亡的影响。
2.On the basis of previous study,in which two target sequences with favorable RNAi effect on HER2 were identified, a series of dual promoter siRNA-expressing vectors c.在以往鉴定的对HER2有良好RNAi效应的靶序列的基础上,构建了一系列U6和H1双启动子小干涉RNA(siRNA)表达载体,并转染HER2高表达乳腺癌SKBR3细胞定量测定了其HER2下调效应。
3.Different siRNA(small interference RNA),as trigger of RNAi, can lead to different level of RNA interference.RNAi)可以抑制诸多真核基因的表达,小干涉RNA(smallintmferenceRNA,siRNA)是RNA干涉的引发物,不同的siRNA可以引导不同水平的RNA干涉,不同种细胞中siRNA的寡核苷酸链的性质也有很大的不同。
6)small interfering RNA小干涉RNA
1.RNAi pathway can be divided into the following steps: assembly of small interfering RNA (siRNA) with the RNA-induced silencing complex (RISC), activition of the RISC, target recognition and cleavage.通过双链小干涉RNA(siRNA)与一系列蛋白质结合形成siRNA诱导的沉默复合体(RISC)并活化,然后,RISC对靶基因进行识别、降解。
2.Thirdly,the resulting insert was ligated into pAVU6+27,a small interfering RNA(siRNA) expression vector.方法 人工合成小鼠肝脏特异的微RNA(miR 1 2 2 )前体基因序列 ,并添加酶切位点及 polyT转录终止序列 ,经退火处理后形成双链结构 ,插入小干涉RNA(smallin terferingRNA ,siRNA)表达载体 pAVU6 +2 7中 ,经酶切鉴定和测序证实后 ,构建成为miR 1 2 2真核表达载体 (pAVU6 +2 7 mir1 2 2 )。
延伸阅读

感染性RNA病原RNA分子式:CAS号:性质:又称感染性RNA病原RNA;壳病毒,是一种和病毒(virus)相似的感染性颗粒。为无蛋白外壳的单链RNA,分子量1.1×105~1.3×105。它是比已知病毒都小的能在宿主细胞内自主复制的病原体之一。已知的近20种类病毒中,大部分已测得了一级结构,都是无蛋白外壳的共价闭合的单链环状RNA分子。在天然状态下类病毒RNA以高度碱基配对的棒状结构形式存在。最先是由T. O. Diener等人(1969)在马铃薯纤块茎病(potato spindle tuber disease)的病株上首先发现的,在电镜下可见到这RNA分子呈50nm长的杆状分子,共有359个碱基对,并证实是游离的RNA,为此正式命名为类病毒。它通常在宿主细胞核内,借助汁液传染,分子量75000~130000,比最小病毒还小80倍。后又相继在菊花矮缩病(chrysanthemum stunt)、菊花绿斑病(chrysanthenum chlorotic mottle)、柑橘剥皮病(citrus excortis)等患病植株中分离到低分子量的病原RNA。推测它也可能存在于其他植物、动物甚至人体内。绝大部分类病毒均具有共同的结构特征:(1)位于棒状结构中心有一个高度保守的序列;(2)靠近这一保守中心区的左侧有一个多聚嘌呤区;(3)棒状结构左侧序列保守性强,右侧变异性大。它可能是通过核苷酸序列或结构改变直接与寄主细胞相互作用、干扰细胞的代谢而致病。对类病毒的研究可能为揭示生命起源和进化、生命过程的实现等生命科学的重大理论问题作出贡献。