1)fetal fibroblast胎儿成纤维细胞
1.The repetitive ITS(internal transcribed spacers) between rDNA genes were used as the target loci for gene targeting to multiple loci in cow fetal fibroblasts.利用奶牛rDNA基因间的ITS重复序列作为靶位点,对奶牛胎儿成纤维细胞进行多位点基因打靶,建立以重复序列为靶位点的多位点基因打靶技术,并为克隆定点转基因奶牛提供核供体。
2.To evaluate the feasibility of gene targeting by homologous recombination in goat fetal fibroblasts, a goat β- casein gene targeting vector pTarget1 was constructed.为评估在山羊胎儿成纤维细胞中通过同源重组进行基因打靶的可行性,构建打靶载体pTarget1,分别以山羊β 酪蛋白基因的5′、3′侧翼区为2条同源臂,中间插入正筛选基因(Neor),外侧插入负筛选基因(HSV tk)。
3.The results are as fallows:Experiment 1: Seven fetal fibroblast cell lines were set up from 7 fetuses of purebred England large white pigs at 40 d of gestation by using explant-seeding method.本研究利用体细胞核移植技术首次成功生产了转ω-3脂肪酸去饱和酶基因(sFat-1)克隆猪,结果如下:实验一:采用组织块培养法,建立了7个38日胎龄的纯种英系大白猪胎儿成纤维细胞系;细胞接触抑制2 d后,经流式细胞术检测发现, 80%以上的细胞处于G0/G1期。
英文短句/例句
1.Culture and sex identification of cattle fetal fibroblasts牛胎儿成纤维细胞的培养及性别鉴定
2.Studies on hALR Transfecting Sheep Embryonic Fibroblasts Cell Mediated by Liposome;脂质体介导hALR转染绵羊胎儿成纤维细胞的研究
3.Determination on Sex of Goat Fibroblast Cell with SRY Gene利用SRY基因鉴定山羊胎儿成纤维细胞的性别
4.Studies Related to Culture of Buffalo Fetal Fibroblasts in Vitro水牛胎儿成纤维细胞体外培养及相关问题的研究
5.Construction of Bovine Transgene t-PA Fetal Fibroblast Cell Lines and Its Nuclear Transfer Embryo;转t-PA基因牛胎儿成纤维细胞系的建立及转基因牛克隆胚的构建
6.Studies on the Factors Influencing Establishment of Cell Line Porcine Embryonic Fibroblasts and Cationic Liposome Mediated Transfection;猪胎儿成纤维细胞建系影响因素与脂质体介导转染法的研究
7.Preparation of Goat Fetal Fibroblast Cell Lines for Nuclear Transfer to Express rHSA in the Chimeric HLA-YAC;用于核移植表达rHSA嵌合HLA-YAC的山羊胎儿成纤维细胞株的制备
8.The Production of Gene-Targeting at GEF β-Casein Gene Locus with Human Lactoferrin Gene and Analysis of Gene Integrate Site;hLF基因打靶山羊胎儿成纤维细胞株的建立及其整合位点的分析
9.Preparation of a Transfected Goat Fetal Fibroblast Cell Stably Integrated Human Lactoferrin cDNA;稳定整合人乳铁蛋白cDNA山羊胎儿成纤维细胞的制备
10.Suppression of FGF5 Expression in Fetal Fibroblasts of Mice by RNA Interference;利用RNAi抑制小鼠胎儿成纤维细胞中FGF5基因的表达
11.The Analysis of Partial Biological Characteristics of Goat Fetal Fibroblast Transfected with hTERT Gene转hTERT基因山羊胎儿成纤维细胞部分生物学性状的分析
12.Study of hTERT Transfected BFF for SCNT Donor转hTERT基因的牛胎儿成纤维细胞作为SCNT核供体的研究
13.The Influence of Human Telomerase Reverse Transcriptase(hTERT) Gene for Goat Fetal Fibroblast人端粒酶逆转录酶(hTERT)基因对山羊胎儿成纤维细胞的影响
14.Construction of MSTN Knock-out Porcine Fetal Fibroblast同源重组敲除MSTN基因的猪胎儿成纤维细胞的构建
15.3. The cell cycle of BFF treated by different methods was examined.3．探讨不同的处理方法和处理时间对水牛胎儿成纤维细胞周期的影响。
16.Observation on Basic Fibroblast Growth Factor and Ultrastructure of Placental Tissue for Late-onset Fetal Growth Restriction晚发型胎儿生长受限胎盘组织碱性成纤维细胞生长因子表达及超微结构的观察
17.Development of mouse blastula into embryonic stem cells on the embryo fibroblast cell feeder layer小鼠囊胚在胚胎成纤维细胞饲养层上向胚胎干细胞的生长发育
18.Studies Related to Stem Cells Derived from Somatic Cells Nuclear Transfer Embryos and Adult Fibroblasts体细胞核移植胚胎和皮肤成纤维细胞来源干细胞的相关技术研究
相关短句/例句
Fetal fibroblasts胎儿成纤维细胞
1.Dissociation culture and subculturing of porcine cumulus cells and fetal fibroblasts;猪卵丘细胞和胎儿成纤维细胞的分离培养及传代
2.The development of nuclear transfer(NT)embryos derived from adult ear fibroblasts, fetal fibroblasts, fetal skin cells and ovary granulosa cells were compared.9%),均显著低于胎儿成纤维细胞(64。
3)embryonic fibroblasts胎儿成纤维细胞
1.Isolation and culturing in vitro of yak embryonic fibroblasts;牦牛胎儿成纤维细胞的分离培养
2.In our experiments, we studied how the factors affected the transfaction efficiency and what effects the liposome method took on sheep embryonic fibroblasts cells in order to increase transfaction efficiency and grand for sheep biology reactor.本文以脂质体介导法转染绵羊胎儿成纤维细胞(sFFCs),寻找影响脂质体介导转染sFFCs效率的因素,以期提高转染效率,为转基因绵羊生物反应器打下基础。
4)fetal fibroblast cell胎儿成纤维细胞
1.Porcine fetal fibroblast cells,ear fibroblast cells and granulosa cells were used as donor in pig cloning.以中国农业大学实验用小型猪香猪胎儿成纤维细胞、成年耳成纤维细胞和颗粒细胞3种细胞系为供体细胞进行核移植。
5)human neofetus dermal fibroblasts人胎儿皮肤成纤维细胞
1.Rat dermal fibrobalasts and human neofetus dermal fibroblasts were cultured in vitro to evaluate the biocompatibility of the chitosan films.为考察壳聚糖对皮肤成纤维细胞的相容性 ,采用流延法制备了纯壳聚糖膜和含甘油的壳聚糖膜 ,并在所制备的壳聚糖膜上进行了大鼠皮肤成纤维细胞和人胎儿皮肤成纤维细胞的体外培养 。
6)sheep fetal fibroblast cell绵羊胎儿成纤维细胞
延伸阅读
成纤维细胞干扰素分子式：CAS号：性质：一种抗病毒蛋白质，也称为成纤维细胞干扰素。分子量25 000~30 000。为糖蛋白，耐酸。在哺乳动物的结缔组织中是成纤维细胞的产物。从胎儿组织或婴儿色皮中分离得到。干扰素也能从培养细胞制取，培养时使细胞在合适的载体如微珠上形成单层，微珠有很高的表面积，可提高产率。抗病毒作用强。
