激光共聚焦显微术,Confocal laser scanning microscopy
Confocal microscopy激光共聚焦显微术
3)laser confocal microscopy激光共聚焦显微术
1.Methods We observed the expression of PKCα in the preimplantation mouse embryo from 2-cell stage to blastula stage by using modified immunofluorescent staining and laser confocal microscopy.方法用优化的免疫荧光化学法结合激光共聚焦显微术,观察小鼠早期胚胎2-细胞期到囊胚期PKCα的表达情况。
4)laser scanning confocal microscopy激光扫描共聚焦显微术
1.By using the laser scanning confocal microscopy and plastic (Leica 7022 histeresin embedding kit) semi thin sectioning technique, comparative studies on the fusion of polar nuclei and the formation of the wall of endosperm cells in autotetraploid and diploid rice were carried out.利用激光扫描共聚焦显微术和塑料包埋半薄切片技术观察同源四倍体水稻极核融合及游离核进一步发育的过程 ,发现同源四倍体水稻约有 1 /3子房极核融合过程和游离核进一步发育与其二倍体原种的基本一致 ;2 /3子房出现各种异常现象 ,如极核未受精、受精异常和胚乳游离核发育滞后等。
2.Using of the laser scanning confocal microscopy technique and combining the H2O2 fluorescence probe H2DCFDA to exam-ine the production H2O2 in guard cell by exogenous ABA, it showed that the fluorescence intensity in the mutant seedlings was lower than that in wild-type ones.采用激光扫描共聚焦显微术(LSCM)并结合H2O2荧光探针H2DCFDA检测外源ABA诱导保卫细胞的结果显示,突变体内荧光强度比野生型拟南芥低,暗示此种突变体消除H2O2的能力可能有提高,从而可增强植株抗氧化胁迫的能力。
5)confocal microscopy激光共聚焦显微技术
6)Confocal laser scanning microscopy共聚焦激光扫描显微术
1.With both two photon laser scanning microscopy and confocal laser scanning microscopy in combination with the fluorescent probe Hoechst 33342, 2′, 7′ dichlorofluorescin diacetate (DCFH DA), Indo 1 and Fluo 3 AM, TCS induced changes in nuclear morphology, reactive oxygen species.应用双光子及共聚焦激光扫描显微术结合特异性荧光探针Hoechst 3334 2、 2′,7′ 二氯荧光黄双乙酸酯(DCFH DA)、Indo 1和Fluo 3 AM ,首次同时观察了TCS诱导人绒癌细胞 (JAR细胞 )凋亡过程中活性氧自由基 (ROS)和细胞内钙离子浓度 ([Ca2 + ]i)的变化 ,实验结果表明TCS引起的 [Ca2 + ]i 升高和ROS形成参与了TCS诱导的JAR细胞凋亡 ,并且ROS形成和 [Ca2 + ]i 升高有关 。
延伸阅读

高分辨电子显微镜(见高分辨电子显微术)高分辨电子显微镜(见高分辨电子显微术)high resolution electron microscope 高分辨电子显微镜high resolution eleetronmieroseope见高分辫电子显微术。