核供体细胞,nuclear donor cell
1)nuclear donor cell核供体细胞
英文短句/例句

1.The Selection of Nuclear Donor Cell in Therapeutic Cloning;治疗性克隆核供体细胞选择的实验研究
2.Development of Embryos Reconstructed with Induced Enucleation Oocytes and Mouse Fetal Fibroblast Cells;小鼠化学去核卵母细胞支持供体细胞核体外发育的研究
3.Preparation of Donor Nuclei by Immunosurgery and Application in Bovine Somatic Cell Nuclear Transfer免疫外科法制备牛供体细胞核胞体及其核移植研究
4.Donor cells play a very important role in nuclear transplantation of Somatic Cell.供体细胞在体细胞核移植中发挥着非常重要的作用。
5.The cell body of a neuron, containing the nucleus and organelles.核周体神经元细胞体,包括细胞核和细胞器官
6.The colorless, liquid component of the cell nucleus.细胞核液细胞核中无色的液体成分
7.Note the presence of megakaryocytes, erythroid islands, and granulocytic precursors.可见巨核细胞,红细胞岛和粒细胞前体细胞。
8.Preparations of Donor and Recipient and Optimization of Fusion and Activation Parameters in Goat Nuclear Transfer;山羊核移植中供体和受体细胞的准备及融合激活参数的研究
9.drum stick nuclear appendage of leucocytes白细胞核棘突鼓锤小体
10.The Influence of Donor Cell Treatments on the Development of Nuclear Transfer Derived Early Embryos of Goat;供体细胞处理方式对山羊核移植胚胎早期发育的影响
11.Regulation of DNA Methylation and Imprinting Gene Expression in Early Pre-Implantation Cloned Mice;小鼠卵细胞质对供体核DNA甲基化和“印记”基因表达的调控
12.Study on the Relationship between Donor Cell Chromatin Histone Acetylation Level and Reconstructed Buffalo Embryos Developmental Potential;水牛供体细胞组蛋白乙酰化与核移植胚胎发育能力相关性的研究
13.Studies on Biological Characteristics and Establishment of Donor Cells Line on Nuclear Transfer in Holstein Cows and Romilly·Hills;荷斯坦奶牛、罗米莉羊供核体细胞系的建立及生物学特性研究
14.Study of hTERT Transfected BFF for SCNT Donor转hTERT基因的牛胎儿成纤维细胞作为SCNT核供体的研究
15.Effect of Trichostatin A on Reprogramming of Bovine Fetal Fibroblast CellsTSA处理供体细胞对组蛋白乙酰化和核重编程效果的影响
16.The Effect of Trichostatin A upon Histone H3 Acetylation in Nuclear Transplantation Donor Cells曲古抑菌素A对核移植供体细胞组蛋白H3乙酰化水平的影响
17.Effect of Different Treatments with Bovine Cumulus Cells as Donor Cells on Cloning Embryos不同处理牛卵丘/颗粒细胞作为核供体对克隆胚发育的影响
18.Production of cloned goats by transfer of nuclei from transfected caprine mammary gland epithelial cells转染乳腺上皮细胞为供核细胞制备克隆羊
相关短句/例句

nuclear donor cells供核细胞
1.Time- and concentration-dependent effect of 5-aza-2 -deoxycytidine and trichostain A on H19 gene expression in nuclear donor cells;5-氮脱氧胞苷及曲古抑菌素A影响供核细胞H19基因表达的时间依赖性及浓度依赖性
3)Nuclear transfer and donor cells核移植与供体细胞
4)Donor cell供体细胞
1.Feasibility of donor cells marked by green fluorescent protein;绿色荧光蛋白作为供体细胞标记物的可行性
2.Effects of derivation and pretreatment of donor cells on the efficiency of nuclear transfer in cattle;供体细胞来源及预处理对牛体细胞核移植效率的影响
3.Dissociation and culture research of porcine donor cell and cell subculture in somatic cell nuclear transfer;猪核移植供体细胞的分离培养及传代的研究
5)donor cells供体细胞
1.To establish human lactoferrin cDNA transgenic donor cell lines and prepare competent donor cells for the production of transgenic animals by somatic cell nuclear transfer (SCNT) and detect the tissue-specific expression of human lactoferrin cDNA, Human lactoferrin cDNA was obtained by RT-PCR while 6.脂质体介导法转染山羊胎儿成纤维细胞,G418抗性筛选3-4周后,经PCR扩增和报告基因EGFP表达检测,得到稳定整合外源基因的转基因供体细胞系;同时在稳定转染p6。
2.To establish humanβ-defensin-3 gene transgenic cell lines as competent donor cells for the production of transgenic animals using somatic cell nuclear transfer (SCNT).最终将构建成功的表达载体通过脂质体法转染奶牛胎儿成纤维细胞和乳腺上皮细胞,转染24-48 h后,在倒置显微镜下观察转染效率;用G418进行抗性细胞克隆的药物筛选,2周后,提取G418抗性细胞克隆的基因组DNA和细胞总RNA,用PCR和RT-PCR方法检测出阳性转基因细胞克隆,鉴定转基因细胞是否整合到细胞的基因组中,以便最终为通过乳腺生物反应器生产药用蛋白的转基因动物提供核移植供体细胞,然后扩大培养,冻存阳性细胞,为下一步进行核移植提供供体细胞;同时,将构建的表达载体转染奶牛乳腺上皮细胞,筛选阳性克隆进行扩大培养,加入胰岛素,氢化可的松和催乳素进行诱导表达。
6)Somatic cell nuclear体细胞核
延伸阅读

核小体分子式:CAS号:性质:是真核生物染色质的基本结构单位。由组蛋白H2A、H2B、H3、H4各两个分子形成扁平圆柱状八聚体,染色质DNA以左手螺旋围绕其上1.75圈约146bp构成。直径11nm,高5.5nm。借组蛋白分子中碱性氨基酸的正电荷和DNA双螺旋中磷酸基负电荷以静电引力维持结构稳定。组蛋白HI在核小体一侧,结合在DNA出入核小体的位点两翼,各覆盖10bp,其功能与染色质进一步集缩有关。有人将包括H1在内的核色质DNA结合长度为166bp的核小体称为染色质体(chromatosome),不包括HI者称核小体的核心颗粒。各染色质体之间为裸露的DNA连接区,其长度因种属而异,一般32~34bp。这种由染色质DNA细丝串联形成的核小体链就是染色体的一级结构,它使染色质DNA在长度上压缩了7~10倍,尚需经多级螺旋化和盘绕折叠,压缩近万倍后才能形成染色体。