1)nucleic acid hybridization核酸杂交
1.In this review,Traditional methods using nucleic acid hybridization technique for genediagnoses are introduced briefly.简要阐述了核酸杂交分析的传统方法和原理，由此对近年来发展起来的ＤＮＡ压电生物传感器、光学生物传感器和光导纤维生物传感器的原理和应用研究作了详细介绍。
2.On the basis of host reactions, serological relationships, peptide mapping of the viral coat protein, dsRNA analysis, nucleic acid hybridization analysis, restriction enzyme analysis of RT PCR products and nucleotide sequence analysis, all strains or isolates of CMV fell into two subgroups.根据寄主反应、血清学关系、病毒外壳蛋白肽链图谱分析、ｄｓＲＮＡ分析、核酸杂交、ＲＴ－ＰＣＲ产物酶解分析及核酸序列分析等方法，可将现有ＣＭＶ株系或分离物区分成２个亚组，尤其是利用单克隆抗体、核酸杂交、ＰＣＲ及核酸序列分析，能准确地将不同亚组的分离物区分开。
3.The principles of DNA sensor based on the principle of nucleic acid hybridization are reviewed.文章从核酸杂交的原理出发介绍了DNA生物传感器的工作原理 ,举例说明了电化学、光学和声学等几种典型的DNA生物传感器 ,指出了其固有的优缺点 ,肯定了DNA传感器发展前
英文短句/例句
1.The Development of a Diagnostic Techenology for Group B Rotavirus by Nucleic Acid Hybridization and Its Preliminary Application;B组轮状病毒核酸杂交检测方法的建立及其初步应用
2.RT-PCR and Dot-Blot Hybridization Detection of Lily Symptomless Carlavirus and the CP Gene Expression in Escherichia Coli;百合无症病毒的RT-PCR和核酸杂交检测及其外壳蛋白在大肠杆菌中的表达
3.Study of Enzyme-amplified Lanthanide Luminescence Time-resolved Fluorescence Detection and Reagents for Nucleic Acid Hybridization Assays;核酸杂交的酶放大镧系元素发光时间分辨荧光分析系统及其试剂的研究
4.Detection of Nucleic Acid of Poultry Virus with Nucleic Acid Probe and Molecule Dot Blot Hybridization Technique;用核酸探针和分子斑点杂交技术检测家禽病毒的核酸
5.Kinetics Model of Nucleic Acid Hybridization Studied by QCM石英晶体微天平研究核酸探针的杂交反应模型
6.The Developing of Potato virus Y Nucleic Acid Spot Hybridization Detection kit;马铃薯Y病毒的核酸点杂交检测试剂盒的研制
7.Application of dual-color fluorescence hybridization chip technique for single nucleotide polymorphism detection in CYP2C19双色荧光杂交芯片技术检测CYP2C19单核苷酸多态性
8.Interspecific hybridization of nucleic acids is a method of this type currently being developed.核酸的种间杂交便是当前正在发展的这样一种方法。
9.Detection of TTV DNA in liver tissue by in situ hybridization应用原位杂交技术检测人肝组织中输血传播病毒核酸
10.Identification the Interacting Partner of Mycobacterium Tuberculosis Isocitrate Lyase by Yeast Two-hybrid;酵母双杂交筛选结核分枝杆菌异柠檬酸裂合酶的相互作用蛋白
11.Enhanced Discrimination of Single LNA Modified Oligonucleotide Probes against Non-Complementary Hybridization应用单个锁核酸修饰的探针提高对非互补杂交识别能力的研究
12.Construction of a New Oligonucleotide Probe Specific for Micromonospora and Optimization of the Hybridization Conditions小单孢菌属特异寡核苷酸探针的设计及杂交条件优化
13.Determination of HPV Genotypes with in Situ Hybridization Technic in Patients with Different Types of Cervical Squamous Epithelial Lesions原位核酸分子杂交技术检测宫颈鳞状上皮病变HPV感染
14.Optimization of hybridization condition of 70mer oligonucleotide probe microarrays for astrovirus detection星状病毒70mer探针寡核苷酸芯片制备及杂交条件的优化
15.Karyotype Analysis of Cymbidium hybridium×C. sinense, C. faberi and their Interspecific Hybrids杂交兰×蕙兰种间杂交后代的核型特征分析
16.Magnetism Bead-oligonucleotide Microarrays-based Multiplex Amplifiable Probe Hybridization Technology Establishment and Application in DMD Detection;磁珠—寡核苷酸微阵列多重可扩增探针杂交技术的建立及在杜氏肌营养不良检测中的应用
17.Selection of Chuan Hybrid Cotton No.19 with High Quality and Insect-resistance优质抗虫核不育杂交棉川杂棉19的选育
18.Fish Based Karyotype Analysis of 21 Gossypium Species棉属21个种基于原位杂交的核型分析
相关短句/例句
Hybridization[英][,haibridai'zei??n][美][,ha?br?d?'ze??n]核酸杂交
1.Objective To study genotypes of HCV RNA using microplate sandwich hybridization.目的 采用微板核酸杂交 ELISA技术对HCVRNA进行基因分型。
2.It is a method to design antisense nucleic acids based on hybridization in order to regulate gene expression artificially.反义技术是根据核酸杂交原理设计反义核酸来人为控制基因表达的方法,现已成为基因工程中的常用技术。
3)nucleotide hybridization核酸杂交
4)in situ hybridization核酸原位杂交
1.Methods One hundred and twenty-five cases of human gliomas were detected for PDGF-B,PDGFR-β,PTEN and p16 mRNA expression by in situ hybridization.方法 应用核酸原位杂交方法检测125例胶质瘤切片中PDGFK B、PDGFR- β、PTEN和p16mRNA表达情况。
2.The analysis through immuno- histochemistry method and in situ hybridization in paraffin block was to detect the expression of FAK, Ezrin, Paxillin, Integrinβ3, TrkC in experimental group and control group, and to stud石蜡封存组织块切片,应用免疫组织化学和核酸原位杂交技术,检测ERK、JNK、FAK、Integrinβ3、Paxillin、Ezrin、TrkC、ER、PR、CerbB-2在实验组、对照组的表达情况,并对实验组、对照组的临床病理因素进行研究。
5)Micro polymerase chain reaction and hybriclitation-ELISA微板核酸杂交-ELISA
6)DNA hybrid probe核酸杂交探针
延伸阅读
核酸（nucleicacid）核酸（nucleicacid）由单核苷酸聚合而成的活细胞中最关键的生物大分子，有DNA和RNA两大类，其组成戊糖不同，碱基则大同小异，DNA是遗传物质基础，RNA起遗传信息表达作用，它直接参与蛋白质的合成。一个核苷酸由三部分组成：碱基、核糖和磷酸。碱基与核糖由核苷键联结成核苷，磷酸与核苷以酯键联结成核苷酸，核苷酸之间由磷酸二酯键聚合成核酸长链。核苷酸参与组成某些辅酶和作为生物能载体的二磷酸核苷（ADP）和三磷酸腺苷（ATP）等，而环状核苷酸（如环腺苷酸cAMP和环-磷酸鸟苷cGMP）是代谢调节中的重要“第二信使”。DNA的二级结构是两条互补的、反向平行的核苷酸链互相盘绕而成的双螺旋。RNA基本上是单股分子，仅局部呈双螺旋结构，分子量比DNA小得多，存在不大的碱基配对区，有转移RNA（tRNA）、信使RNA（mRNA）和核蛋白体RNA（rRNA）。核酸是高分子量、高黏度、易沉降、等电点低的酸性物质，在波长260nm下呈最大紫外吸收。核酸常与蛋白质结合成核蛋白，比较重要的核组蛋白有染色质、核蛋白体和病毒等。
