反转录PCR,RT-PCR英语短句,例句大全

反转录PCR,RT-PCR
1)RT-PCR反转录PCR
1.Methods: The expression of 5-HT receptor subtype mRNAs was detected in the lumbar dorsal root ganglion (DRG) by reverse transcription-polymerase chain reaction(RT-PCR) following unilateral injection of formalin into the plantar surface of rat hind paw.方法:用反转录PCR技术观察大鼠单侧足底皮下注射福尔马林致痛后背根节内5-HT1~7受体亚型mRNAs的表达变化。
2.?Methods Using the total RNA extracted from fresh rat hippocampus as template,the cDNA of PDZ1 was amplified by RT-PCR;the cDNA obtained was subsequently cloned into the shuttle vector pAdTrack-CMV(CLONTECH),and then,the sequence of the product was determined.方法以异硫酸氢胍-酚-氯仿一步法提取的总RNA为模板,采用反转录PCR(RT-PCR)法获得PDZ1的cDNA;与腺病毒穿梭载体用T4DNA连接酶连接;连接产物转化大肠杆菌JM109进行筛选、序列测定。
3.Methods:BMP7 gene was cloned from the HEK293 cells with RT-PCR and nested-PCR skills,and after being repaired,the whole cDNA of BMP7 was obtained.方法 :通过反转录PCR技术和巢式PCR技术从人类胚胎肾 2 93细胞中克隆BMP7基因 ,应用互补拼接的方法对其进行修复获得全长cDNA ,将其与T载体进行连接得到BMP7克隆质粒。

英文短句/例句

1.The identifition of SRC-1 gene by differential display RT-PCR related to intramuscular fat deposition in Sutai pigs苏太猪肌内脂肪沉积相关基因SRC-1的差异显示反转录PCR鉴定
2.Study on PCR Reaction System of Inter-retrotransposon Amplified Polymorphism(IRAP) in Lentinula edodes香菇反转录转座子间扩增多态性(IRAP)PCR反应体系的研究
3.Detection of potato black ringspot virus by semi-nested RT-realtime PCR.半巢式反转录实时荧光PCR检测马铃薯黑环斑病毒
4.1.2.3 RT-PCR reaction and agarose gel electrophoresis: take forementioned reversion record reaction product to 25 l reactant intermediate to proceed PCR reaction.2.3 RI’- PCR反应和琼脂糖凝胶电泳:取上述逆转录反应产物于25川反应体
5.reverse transcriptase【生化】逆转录酶, 反转录酶
6.Development of an Antigen Capture Reverse Transcriptase/Polymerase Chain Reaction for Detection Foot-and-Mouth Disease Virus;抗原捕获反转录聚合酶链式反应（AC-RT/PCR）检测口蹄疫病毒方法的建立
7.Detection of rubella virus gene sequences by RT-nested PCR用逆转录套式PCR检测风疹病毒RNA
8.cyclical transcription and reverse transcription循环（式）转录（和）逆转录（反应）
9.Detection of SARS-CoV from feces samples with RT-PCR逆转录套式PCR检测粪便样本中的SARS冠状病毒
10.Detecting Highly Pathogenic Avian Influenza Virus H5N1 Subtype for Human Being by RT-Multiplex PCR逆转录多重PCR检测人感染高致病禽流感H5N1亚型
11.Cloning of Reverse Transcriptase of Retrotransposons and Stress-Tolerant Related Gene in Wheat;小麦反转录转座子反转录酶和抗逆相关基因的克隆
12.Cloning, Sequencing and Detecting by PCR-SSCP of TTF-1 in Sheep;绵羊甲状腺转录因子-1（TTF-1）基因克隆、序列分析及PCR-SSCP检测
13.Research on Radiation Carcinogenesis Related Genes with Single Cell RT-PCR and Suppressive Subtractive Hybridization;应用单细胞逆转录PCR和抑制差减杂交技术研究辐射致癌相关基因
14.Detection of PML/RARα Fusion Gene Transcripts in Patients with APL Using Real-time Quantitative PCR实时定量PCR检测急性早幼粒细胞白血病患者PML/RARα融合基因转录本
15.Quantification of PRAME transcripts in patients with acute myeloid leukemia using real time PCR with EvaGreen dyeEvaGreen染料法实时定量PCR检测急性髓系白血病PRAME基因转录本
16.Measurement of the Forkhead Box P3 Gene Expression Levels in Esophageal Squamous Cell Carcinoma by Real-time Quantitative Reverse Transcription-polymerase Chain Reaction定量RT-PCR测定食管鳞癌组织中人叉头样转录因子3基因的表达
17.Research on Exogenous Gene mRNA Levels in Zymomonas mobilis by Semi-quantitative RT-PCR半定量RT-PCR检测运动发酵单胞菌中外源基因转录水平的研究
18.Detection of BAALC expression in bone marrow of acute myeloid leukemia patients by real-time quantitative reverse transcriptase polymerase chain reaction实时定量逆转录PCR检测急性髓系白血病患者骨髓细胞BAALC基因表达

相关短句/例句

RT PCR反转录PCR
1.cDNA fragment encoding human P34H was amplified by RT PCR using specific primers, and then was cloned into pGEM T vector.　方法 :提取人附睾体部总RNA ,并以此为模板 ,进行反转录PCR获得编码P34H蛋白的基因片段。
2.Methods:Tumor tissues and adjacent normal tissues in 41 cases of esophageal carcinoma were studied by using the reverse transcriptase polymerase chain reaction(RT PCR).方法 :用反转录PCR(RT PCR)法检测 41例食管癌患者的癌组织、癌旁组织MMP 2mRNA的表达。
3)Reverse transcription PCR反转录PCR
4)reverse-transcription PCR反转录PCR
5)RT-PCR反转录-PCR
1.0kb DNA fragment was obtained by RT-PCR fr.本研究以黑曲霉M-1为出发菌株，设计特异的引物，采用反转录-PCR技术直接从黑曲霉总RNA中反转录并扩增出约3。
6)in situ RT-PCR原位反转录PCR
1.The principle, key factors and steps of in situ RT-PCR including tissues or cells fixation, primer and probe selections, protease or DNase digestions, false positive and false negative results were summarized to emphasize the application of in situ RT-PCR to the actual detections.针对已有资料中对于以DNA为靶基因序列的原位 PCR介绍较多,而对于原位反转录PCR的介绍很少,但在实际研究工作中有很多检测的靶基因是 RNA(病毒的或基因组的 RNA)的实际情况,就原位反转录PCR技术的基本原理、影响试验结果的关键因素和步骤,如组织细胞的固定、引物和探针的选择、蛋白酶及DNA酶消化、假阳性及假阴性的产生等进行了概述,旨在通过探讨上述问题,对该技术的实际应用有所指导。