1)electroporation电转染
1.Employing electro-competent cells prepared from early stationary phase mycelia cultured in MYM, a specially modified YEME medium and optimized electroporation conditions, a transformation efficiency of 5x105 pfu/(g phage DNA was obtained.利用一株地中海拟无枝菌酸菌专一噬菌体φMMR1的DNA电转染U-32导入过程作为模型，通过对影响噬菌体DNA电转染U32效率的因素特别是菌体的生长条件、电击条件的优化，确定了一系列导致高频电转染的参数。
英文短句/例句
1.Parameter Optimization in the Electroporation for Introduction of Human Ribosomal Targeting Vector into Hepatocyte;核糖体打靶载体电转染肝细胞条件的优化
2.Study of Anti-tumor by Dendrtic Cells Electransfected Total RNA of Human Hepatic Cancer Cell;人肝癌细胞总RNA电转染树突状细胞的抗肿瘤研究
3.Experimental Studies on Establishing Mouse Embryonic Stem Cell Lines and Electroporating K14-EGFP Gene to the Mouse Embryonic Stem Cells in Vitro;小鼠胚胎干细胞体外建系及电转染K14-EGFP基因的实验研究
4.The Investigation of hGM-CSF Gene Transfer by in Vivo Electroporation to Remedy the Radiation Injury of Mice;hGM-CSF基因活体电转染救治小鼠放射损伤的实验研究
5.Nave T Cell Transfected with Recombinant Human Vector Eukaryotic pIRES-EGFP-BCL 11B Studied by Atomic Force Microscope重组质粒pIRES-EGFP-BCL 11B电转染幼稚T细胞的可视化研究
6.Analyze the rate of lymphocyte apoptosis in mice suffering from radiation damage of transferred GM-CSF gene with electroporation and those of without transferred GM-CSF gene by flow cytometryhGM-CSF基因活体电转染小鼠中子辐射损伤后细胞凋亡流式分析
7.Nucleofector~(TM) electrotransfection promotes senescence of human embryo lung fibroblasts电转染技术促进人胚肺二倍体成纤维细胞的衰老进程
8.The Kinetic Study of the Ultrafast Electron Transfer from Sensitized Dyes to Silver Halide Microcrystals;卤化银染料增感体系超快电子转移动力学研究
9.Study on Conversion Oflighttoelectricity of Nanocrystalline TiO_2 Films Doped Moified by Dyestuff;染料敏化纳米晶TiO_2薄膜的光电转换性能的研究
10.Study on Monitoring Electrophile Chemical Pollutant Based on Transgenic Cell;构建转基因细胞检测亲电性化学污染物的研究
11.The Isolating Culture of Neural Stem Cells from Rat's Fetus Brain and Transfection by Electroporation大鼠胎脑神经干细胞的分离培养及电穿孔转染
12.Applications of Up-Conversion Luminescence in Dye Sensitized Solar Cell上转换发光在染料敏化太阳能电池中的应用
13.Influence of resistance of TiO_2 mesoporous films on photoelectric properties of dye-sensitized solar cellsTiO_2介孔薄膜电阻对染料敏化太阳能电池光电转换性能的影响
14.Research on Electrokinetic Remediation of Aniline-Polluted Soil Influenced by Adding Solutions and Rotational Electrodes添加剂及旋转电极对电动修复苯胺污染土壤影响的研究
15.Eenhanced photoelctric conversion efficiency in dye-sensitized solar cells by back reflection用背反射提高染料敏化太阳能电池光电转换效率的研究
16."After such a combination treatment, her electrolyte imbalance improved, But her lung infection and acid-base imbalance were still not completely corrected.""经过综合治疗.电解质率乱好转,但肺部感染用酸碱失衡未能纠正"
17.Acridine Dyes Sensitized Electron Transfer Photoisomerization of Norbornadiene Derivatives降冰片二烯衍生物的染料敏化电子转移光异构化反应的研究
18.The Research on the Law of Tide Induced Dispersion of the Liquid Radioactive Wastes of Daya Bay Nuclear Power Station and on the Law of Radionuclide Transfer in the Marine Ecosystems;大亚湾核电液态排放的潮流污染扩散及海洋生态转移研究
相关短句/例句
electrotransfection电转染
1.Objective: To explore the effect of influencing factors on electrotransfection efficiency.目的:探讨影响电转染效率的因素。
2.The changes of cell s permeability, electrotransfection, electrofusion and the change of cell shape were studied.本实验采用超宽频带横电磁传输室(BTEM Cell)在低强度瞬态电磁场(Low-intensity Transient Electromagnetic Pulses, LTEMP)作用下，分别从细胞膜通透性的变化，电转染，电融合以及细胞膜的表面形态变化等几个方面研究了LTEMP导致细胞膜穿孔的生物学非热效应。
3)Electransfection efficiency电转染率
4)DNA ElectrotransferDNA电转染
5)Electroporation In Vivo体内电转染
1.Electroporation In Vivo Enhances the Humoral Immune Re-sponse against HBV-preS2/S following Gene Immunization;体内电转染增强基因免疫诱导的HBV特异性体液免疫应答
6)electroporation电穿孔转染
1.Objective To optimize the electroporation parameters in mouse primary lymphocyte cultured in suspension with adenosine deaminase acting on RNA 1(ADAR1) as a reporter gene.目的以RNA依赖性腺嘌呤脱氨酶(ADAR1)为靶基因优化悬浮培养的小鼠原代淋巴细胞的电穿孔转染条件。
2.It turns out that 250mg/L carrier DNAincreases the expression level by 3 fold,andlinearized plasmid DNA is more suitable thansupercoiled DNA to obtain stable transfectionby electroporation.以外源红细胞生成素ｃＤＮＡ的表达产物为指标，研究了运载ＤＮＡ和重组表达质粒的构象对电穿孔转染ＣＨＯ细胞的效率的影响。
延伸阅读
电转儿〈方〉电唱机。
