定点突变体,Mutants英语短句,例句大全

定点突变体,Mutants
1)Mutants定点突变体
1.Thermodynamic Stability of Hen Egg White Lysozyme and Its Mutants by Site directed Mutagenesis;卵清溶菌酶及其定点突变体热力学稳定性研究

英文短句/例句

1.Study on Activity of Mutant Staphylococcal Enterotoxin B with Histindine Substitution;金黄色葡萄球菌肠毒素B定点突变体活性的研究
2.Construction and Function of Site-directed Mutant of Adinbitor, A Disintegrin from Gloydius blomhoffi brevicaudus白眉蝮蛇去整合素Adinbitor定点突变体的构建及其功能检测
3.Using SSR Marker to Map the Mutant Sites of the Wheat Salt-Tolerant Mutant and the Study on the Difference of Its mtDNA;小麦耐盐突变体突变位点的SSR标记定位及其线粒体DNA差异的研究
4.Molecule-Directed Evolution and Site-Directed Mutagenesis of D-Hydantoinase;D-海因酶的体外分子定向进化和定点突变
5.The Expression Vector Construction and Site-directed Mutation in Vitro of RET Oncogene;RET基因真核表达载体的构建及其体外定点突变
6.Site-direct Mutation and Eukaryotic Expression of the Human Growth Hormone Receptor;人生长激素受体的定点突变及其真核表达
7.Improve the Enantioselectivity of Lipase A of Bacillus Subtilis by Site-directed Mutagenesis;定点突变提高枯草杆菌脂肪酶A的对映体选择性
8.Construction and Identification of Recombinant hING4 Recombinant Adeno virus Mediat ed Gene Transfer Syst em by Site-Directed Mutagenesis定点突变重组ING4腺病毒基因转染系统的载体构建及鉴定
9.Identification of Key Amino Acid Residues in Human CYP2A6 and CYP2A13 for Coumarin 7-Hydrocylation Metabolism by Site-Directed Mutagenesis定点突变法确定人体CYP2A6和CYP2A13香豆素代谢的关键氨基酸
10.Site-directed Mutagenesis and Random Mutagenesis of Penicillium Expansum Lipase;扩展青霉脂肪酶的定点突变及随机突变
11.Spotting the Positions on the Genome for 4 Pathogenicity-related Mutagenic Sites of Magnaporthe Grisea and Analyzing on a Peg Deficiency Mutant;稻瘟菌四个致病相关突变位点的定位及侵染钉缺陷突变体的初步研究
12.Construction of Phage-displayed Site-directed Random Mutation Combinatorial Library of Immunoglobulin-binding Molecules;免疫球蛋白结合分子定点随机突变组合噬菌体展示文库的构建
13.The New Type of Recombinant Pisum Sativum Prophobilinogen Deaminase and Site-directed Mutagenesis;豌豆胆色素原脱氨酶新型表达载体构建与定点突变
14.Site-directed Mutagenesis of Sumf 1 Gene of Mouse Based on Overlap Extension PCR and Construction of Eukaryotic Expression Vector重叠延伸PCR法构建小鼠Sumf1基因的定点突变真核表达载体
15.Construction of Soybean Mutant Pools and Characterization of Different Mutant-Types;大豆突变体库的初步构建及突变类型的鉴定
16.Overexpression and Site-directed Mutagenesis of cry1Aa Gene from Bacillus Thuringiensis苏云金杆菌cry1Aa基因的表达和定点突变
17.Site-Directed Mutagenesis Analyses, Codon Optimization Study of Harpin_x Protein;Harpin_x蛋白定点突变和密码子优化研究
18.AN IMPROVEMENT ON SITE-DIRECTED MUTAGENESIS USING PCR利用PCR进行基因定点突变方法的改进

相关短句/例句

site-directed mutagenesis定点突变
1.Site-directed mutagenesis of functional gene fragment producing CTX-M-14-type extended-spectrum β-lactamase;CTX-M-14型超广谱β-内酰胺酶基因的定点突变研究
2.Study on site-directed mutagenesis and kinetics of trehalose synthase from Thermobifida fusca;Thermobifida fusca海藻糖合成酶的定点突变及其动力学性质研究
3.The Expression of hBmp-4 in the Pichia Pastoris and Its Site-Directed Mutagenesis;hBmp-4在甲醇酵母中的表达及其定点突变
3)Site directed mutagenesis定点突变
1.Using recombinant glucagon expression system,the 21st aspartic acid was changed to be alanine by means of site directed mutagenesis.在获得重组胰高血糖素基因工程菌基础上 ,利用定点突变技术改造其第 2 1位氨基酸天冬氨酸为丙氨酸 ,并经DNA测序证明胰高血糖素基因发生了点突变。
2.Two p 16 mutants, P48L and D74N, were obtained by site directed mutagenesis using two step PCR method.应用ＰＣＲ体外定点突变技术，构建了ｐ１６－Ｐ４８Ｌ和ｐ１６－Ｄ７４Ｎ突变体。
3.Human brain derived neurotrophic factor gene was used as template for oligonucleotide based site directed mutagenesis and PCR.应用寡核苷酸诱导的定点突变和ＰＣＲ技术对人脑源性神经营养因子基因进行突变，并完成了测序鉴定。
4)site-directed mutation定点突变
1.A site-directed mutation at 2753 bp of the ret cDNA induced by PCR;PCR介导的ret基因体外定点突变
2.Four conservative tryptophan sites were selected to actualize site-directed mutation.选取了四个色氨酸保守性位点,分别进行定点突变,构建了CpeS的四个色氨酸突变体,分别为CpeS(W14I)、CpeS(W69M)、CpeS(W75S)和CpeS(G119C,W140V)。
3.OBJECTIVE: To obtain sequence of α—glucosidase, SWISS-MODEL Server predicted that enzyme structure, amino acid property and characteristic, to design molecular of alpha-glucosidase,make two site-directed mutation by TaKaRa MutanBEST Kit,express the mutant gene in E.目的:使用PCR技术获得α—葡萄糖苷酶基因序列,通过SWISS-MODEL服务器对α—葡萄糖苷酶结构进行预测,结合氨基酸的性质特点,对酶蛋白进行分子设计,采用PCR突变试剂盒对其进行定点突变,并在大肠杆菌中表达,测定了重组菌、突变菌的酶活力,为进一步研究α—葡萄糖苷酶结构与功能的关系奠定了基础。
5)site-specific mutagenesis定点突变
1.Therefore, the method of site-specific mutagenesis was used to turn conservative asparagines into hydrophobic .基于此,采用定点突变技术对该基序中保守的天冬酰胺进行了一系列的疏水性缬氨酸突变和亲水性的谷氨酰胺突变,并将这一系列突变体克隆至原核表达载体pET-11a中,在大肠杆菌BL21(DE3)中进行了高效表达与初步纯化,为后续测定其热滞活性的变化和验证天冬酰胺的重要性打下了基础。
6)point mutation定点突变
1.Methods PCR point mutation was induced by mutating primer and gene vector of PDIP1,the product of PCR and vector pGEX-4T-2 were digested by EcoRⅠand SalⅠ,the pGEX-4T-2-PDIP1 was ligased by the objective segments and them.方法利用定点突变PCR对PDIP1编码序列进行定点突变,然后将PCR定点突变产物和pGEX-4T-2载体经EcoRⅠ与SalⅠ酶切,再利用连接酶将它们定向连接起来形成pGEX-4T-2-PDIP1重组质粒,转化E。