PCR,Polymerase chain reaction
1)Polymerase chain reactionPCR
1.Construction of the standards for detecting human hepatocyte growth factor mRNA with real-time fluorescence quantitative polymerase chain reaction;人肝细胞生长因子mRNA实时荧光PCR定量标准的构建
2.Detection of Mycoplasma gallisepticum and M. synoviae by polymerase chain reaction;PCR检测鸡毒霉形体和鸡滑液囊霉形体的研究
英文短句/例句

1.Development of a multiplex PCR/RT-PCR assay for detection of porcine reproductive and respiratory syndrome virus,porcine parvovirus,pseudorabies virus and porcine circovirus type 2多重PCR/RT-PCR技术检测PRRSV、PPV、PRV和PCV-2
2.Rapid detection of canine parvovirus by Real-time PCR and PCR methodsReal-time PCRPCR方法快速检测犬细小病毒
3.Diagnosis Establishment Formulation of RT-PCR、Fluorescence Quantitative PCR and Mutiplex RT-PCR Detection for Japanese Encephalitis Virus;猪乙脑病毒RT-PCR、荧光定量PCR及多重RT-PCR诊断方法建立
4.Advance in PCR-SSCP and Its ApplicationPCR-SSCP技术应用研究进展
5.Rapid Detection of Avian Influenza Virus by Diplex RT-PCR and Real-Time RT-PCR;禽流感病毒双重RT-PCR及荧光RT-PCR快速检测
6.Detection of Five Citrus Diseases by RT-PCR and Multiplex RT-PCR;五种柑橘病害的RT-PCR及多重RT-PCR检测研究
7.A study on Genetic Polymorphism of GPT loci by FLDAS-PCR and PCR-RFLP;用FLDAS-PCRPCR-RFLP检测汉族人群GPT的多态性
8.A QUICK AND MORE SENSITIVE METHOD TO IDENTIFY PORK IN PROCESSED AND UNPROCESSED FOOD BY PCR AND REALTIME-PCR METHOD高效快速检测食物中猪成分的PCR和RT-PCR方法
9.Detection of Impatiens necrotic spot tospovirus(INSV) by RT-PCR and Nested PCR凤仙花坏死斑病毒的RT-PCR和巢式PCR检测
10.PCR Detection and in Situ PCR Location of Specific DNA from Sugarcane Parental Species;甘蔗亲本种特异DNA序列的PCR检测及原位PCR定位的研究
11.Establishment of Nested RT-PCR and Real-Time Fluorescence RT-PCR Methods to Detect West Nile Virus;西尼罗病毒套式RT-PCR和实时荧光RT-PCR检测方法的建立
12.Comparative Analysis of Fluoresenes Quantitative-PCR and Reverse Transcription-PCR in Detecting HCV-RNA;荧光定量PCRPCR酶免检测丙型肝炎病毒核酸的比较分析
13.Research of RT-PCR and Multiplex RT-PCR for Detection of H5N1 Subtype of Avian Influenza Virus;H5N1亚型禽流感病毒RT-PCR及多重RT-PCR检测技术的研究
14.Detection of Tilletia Controversa Kühn by PCR and Real Time Quantitative PCR;小麦矮腥黑穗菌常规PCR与实时荧光定量PCR检验检疫技术研究
15.Development of Nested-PCR and Real-time PCR Method for Diagnosing Anaplasmosis;牛无浆体病套式PCR及实时荧光PCR检测方法的建立
16.A PCR and Real-time Fluorogenic Quantitative-PCR for Rapid Identification of Bee Chalkbrood Disease;蜜蜂白垩病PCR及荧光实时定量PCR诊断方法的建立
17.Establishment of Nested PCR and Real-Time PCR Methods to Detect Infectious Bovine Rhinotracheitis Virus;牛传染性鼻气管炎病毒套式PCR和荧光PCR检测方法的建立
18.Establishment of Rapid Diagnostic Methods for Detection of West Nile Virus by Nest RT-PCR and TaqMan Real-time RT-PCR;西尼罗病毒巢式RT-PCR和荧光定量RT-PCR两种检测方法的建立
相关短句/例句

polymerase chain reaction(PCR)PCR
1.Using the primers,7 LABs were identified by polymerase chain reaction(PCR) amplification of the 16S rDNA.并用该引物对从啤酒厂分离到的7种乳酸菌进行了检测,PCR结果表明该引物能够准确检测到啤酒中常见的乳酸菌。
2.Cerebrospinal fluid specimens were boiled or extracted DNA by the extraction kit and crgA gene was detected by Polymerase Chain Reaction(PCR).[方法]以煮沸法裂解细菌获取PCR反应模板,或以基因组DNA纯化试剂盒提取DNA后,用PCR法扩增脑膜炎奈瑟菌crgA基因;对PCR产物进行DNA测序。
3.A 16S rRNA-based polymerase chain reaction(PCR) method was used in th.方法:分别提取26例根管预备后约诊期间炎症急性发作患牙根管样本和23例慢性根尖周炎患牙根管样本,提取样本细菌DNA,利用细菌16S rRNA引物通过PCR扩增方法鉴定细菌。
3)Polymerase Chain Reaction (PCR)PCR
1.Sex determination of bovine embryo using consecutive and multiplex polymerase chain reaction (PCR);连续多重PCR牛胚胎性别鉴定
2.Some of these techniques, such as Fluorescent in situ Hybridization (FISH), Polymerase Chain Reaction (PCR), DNA cloning and DNA sequencing, were reviewed.综述了荧光原位杂交(FISH)、多聚酶链式反应(PCR)、DNA克隆及DNA测序等分子生物学技术,并将这些分子生物学技术应用到淡水水体底泥厌氧氨氧化菌(anammox菌)和好氧氨氧化菌的原位检测中,从底泥样品中鉴别出这两种细菌,其中好氧氨氧化菌属于亚硝酸单胞菌属,厌氧氨氧化菌属于anammox菌的Brocadia分支,为进一步研究淡水环境中氮的微生物循环过程提供了一定的依据。
3.Objective To develop a polymerase chain reaction (PCR) for specifically detecting all serogroups of leptospira interrogans epidemic in China.目的 建立一种能特异检测我国致病性钩端螺旋体所有血清型的PCR方法。
4)PCR-RFLP and PCRPCR-RFLP及PCR
5)polymerase chain reaction(PCR) apparatusPCR机
6)Realtime RT-PCRRealtimeRT-PCR
延伸阅读

PCR分子式:CAS号:性质:又称基因体外扩增技术或核酸体外扩增技术。利用两种与相反链杂交并利用附着于目标DNA两端的寡核苷酸引物在高温(95℃)使含目标DNA片段的DNA双链变性,分解为单链。在较低温度(37~63℃)与引物退火:然后变温到72℃左右,在耐高温DNA聚合物酶作用下引物被沿样板DNA单链延伸合成互补链,然后又变性→退火→延伸反复进行。引物大大过量,dNTP过量,酶在高温中是较稳定的,这样经过几十个循环,目标DNA片段就按2n数递增。用此法可从mRNA中,cDNA库中扩增出目标基因。过去一般合成500~1000bp较好,现已发展出大片段(75kb)的PCR,且差错甚少。PCR技术的发展还可用于定点突变,质粒重组及FLP等方面。这一技术的作用范围日益扩大,已成为分子生物学工作者基本技术之一。这项专利技术1985年由美国塞特斯(Cetus)公司开发。是20世纪80年代分子生物学领域中的一项革命性突破,已在分子生物学、医学、法学等领域发挥了极大的作用。