1)RNA-dependent RNA polymeraseRNA依赖的RNA聚合酶
1.Yeast expression plasmid containing hepatitis C virus nonstructural gene 5b from infection clone was constructed and transformed into Pichia pastori,constitutive expression of soluble HCV RNA-dependent RNA polymerase(RdRp)was obtained and indicated to be special 64.构建含有感染性克隆HCV非结构基因 5b区序列的酵母表达质粒 ,转化毕加酵母 ,获得持续、可溶性HCVRNA依赖的RNA聚合酶 (RdRp)的表达 ,纯化蛋白在SDS -PAGE及Westernblot中显示出特异性的 6 4 2kDHCVRdRp蛋白带 ,同聚引物 /模板测定显示RdRp的活性极低 ,延长反应时间 ,RNA聚合活性仅轻度升高。
2.The 3D gene encoding RNA-dependent RNA polymerase(RdRp) of foot-and-mouth disease virus was amplified by RT-PCR using pfu ultra~(TM) High-Fidelity DNA polymerase,and the amplicon was cloned into the eukaryotic expression vector pcDNA3.利用RT-PCR技术扩增口蹄疫病毒(FMDV)编码RNA依赖的RNA聚合酶的3D基因,经测序确认后将其克隆到真核表达质粒载体pcDNA3。
3.The full-length NS5 (NS5F) and the NS5 fragment encoding the RdRp, RNA-dependent RNA polymerase, domain (NS5pol) were PCR amplified from a full-length WNV cDNA clone and cloned into the prokaryotic expression vectors pET-28a, respectively.以病毒全长cDNA克隆为模板,PCR扩增获得NS5的RNA依赖的RNA聚合酶(RdRp)活性区(NS5pol)及该蛋白完整的编码序列(NS5F),分别克隆于原核表达载体pET-28a并转化至大肠杆菌E。
英文短句/例句
1.Activity Assay and Inhibitor Screening of HCV RDRP;HCV RNA依赖的RNA聚合酶（RDRP）活性测定及抑制剂筛选
2.Initiation of Genomic RNA Replication by a Recombinant Classical Swine Fever Virus RNA-dependent RNA Polymerase;猪瘟病毒RNA依赖的RNA聚合酶起始基因组RNA复制的分子机制研究
3.Isolation and Function Identification of a Novel RNA-dependent RNA Polymerase Gene, GhRdRP, in Cotton (Gossypium Hirsutum L.);棉花RNA依赖的RNA聚合酶（GhRdRP）基因的分离及功能分析
4.Cloning and Expression Analysis of an RNA-dependent RNA Polymerase Gene, NgRDR1, from Nicotiana Glutinosa心叶烟RNA依赖的RNA聚合酶基因（NgRDR1）的克隆及其表达特性研究
5.The Expression and Purification of SARS-CoV RNA Dependent RNA PolymeraseSARS冠状病毒RNA依赖的RNA聚合酶原核和真核表达及纯化
6.Studies of the Distribution of Avian Influenza Virus RNA-dependent RNA Polymerase PA、PB1 and PB2 Subunit in Cells Cytoplasm and Preparation of Monoclonal Antibody流感病毒RNA依赖的RNA聚合酶亚单位的细胞定位研究及单克隆抗体制备
7.dna dependent rna polymerase依赖dna的rna聚合酶
8.Expression of Double-stranded RNA-dependent Protein Kinase in Cervical Lesion Tissues宫颈病变组织中双链RNA依赖的蛋白激酶表达
9.Advances in the Study of the σ~S (RpoS) Subunit of RNA PolymeraseRNA聚合酶σ~S亚基的研究进展
10.RNA-dependent DNA polymerase依赖于ＲＮＡ的ＤＮＡ聚合酶
11.deoxyribonucleic acid-dependent DNA依赖于ＤＮＡ的ＤＮＡ聚合酶
12.Relationship Between the Expression of PKR and High Risk HPV in Human Cervical Cancer and Pre-malignant Lesion宫颈病变中双链RNA依赖的蛋白激酶与HPV感染的相关性
13.Eukaryotic Expression,Purification and Identification of Double-stranded RNA-Dependent Protein Kinase(PKR)双链RNA依赖的蛋白激酶(PKR)的真核表达、纯化及鉴定
14.Establishment of a ST Cell Line Stably Expressing T7 RNA Polymerase;稳定表达T7 RNA聚合酶的ST细胞系的建立
15.Detection of HCV RNA using Tth DNA polymeraseTthDNA聚合酶在丙型肝炎病毒RNA检测中的应用
16.Studies on the Nuclear Actin in Association with RNA Polymerase Ⅱ;细胞核肌动蛋白与RNA聚合酶Ⅱ关系的研究
17.Functinal Characterization of the Omega Subunit of the RNA Polymerase of Xanthomonas Campestris Pv. Campestris;十字花科黑腐病菌RNA聚合酶ω亚基的研究
18.Studies on the Localization of RNA Polymerase Ⅲ Transcription in HeLa Cell Nuclei;RNA聚合酶Ⅲ在HeLa细胞核中转录位点的研究
相关短句/例句
RNA-dependent RNA polymerase依赖于RNA的RNA聚合酶
1.In the structural proteins, the P1 was the putative RNA-dependent RNA polymerase, which might play an important role in the synthesis of RGDV genomic dsRNAs;The P5 bound with GTP and UTP was a NTP binding protein with RNA guanylyltransferase activity that synthesized a cap structure (mGpppAmpGp) for.采用α 32 P标记的三磷酸核苷酸与病毒粒子共浴的方法及Northwestern分子杂交方法 ,研究证明了RGDV结构蛋白中 ,P1蛋白可能是依赖于RNA的RNA聚合酶 ,P5蛋白可能具有RNA鸟苷酰转移酶功能 ,P6蛋白可能是核酸结合蛋白 ,是RGDV复制包装中的关键蛋白。
3)RNA-dependent RNA polymerase依赖RNA的RNA聚合酶
1.The RNA-dependent RNA polymerase(RdRp) is the viral replicase.正链RNA病毒的基因组为正链RNA,其复制酶是依赖RNA的RNA聚合酶,非编码区是病毒基因组复制的主要调控位点,3’非编码区是复制酶的第一结合位点,正链RNA病毒基因组大多可能按copy-back模型进行复制。
4)RNA-dependent RNA polymerase(RdRp)依赖于RNA的RNA聚合酶
1.Cloning of RNA-dependent RNA polymerase(RdRp) gene of HCV and its expression in E.coli system;丙型肝炎病毒依赖于RNA的RNA聚合酶基因克隆与原核表达
5)RNA dependent RNA polymerase(RdRp)依赖RNA的RNA聚合酶(RdRp)
6)NS5B RNA-dependent RNA polymeraseNS5B RNA依赖的RNA聚合酶
1.Hepatitis C virus (HCV) NS3/4A serine protease and NS5B RNA-dependent RNA polymerase are vital for the processing and maturation of viral protein precursors and RNA replication.丙型肝炎病毒(hepatitis C virus,HCV)NS3/4A丝氨酸蛋白酶和NS5B RNA依赖的RNA聚合酶是病毒蛋白前体加工成熟和复制过程中十分重要的两个酶,是抗HCV治疗的理想靶点。
延伸阅读
RNA 见核糖核酸。
