半胱天冬蛋白酶-3,caspase-3
Caspase-3半胱天冬氨酸蛋白酶-3
1.Objective: Investigating the expression of Caspase-3 mRNA by reverse transcription polymerasechain reaction(RT-PCR) in the normal and preeclampsia placentas in order to evaluate the role of Caspase-3 in preeclampsia.目的检测半胱天冬氨酸蛋白酶-3(Caspase-3)mRNA在子痫前期患者胎盘中的表达,以探求Caspase-3在子痫前期发病机制中的意义。
2.Eventually resistant tumor cells manifest as inhibition of the activation of Caspase-3,and reduction of apoptosis.耐药肿瘤细胞半胱天冬氨酸蛋白酶-3(Caspase-3)信号传导通路发生缺陷,使Caspase-3活化受抑,最终导致凋亡减少。
3.To investigate the effect of hydrogen peroxide(H2O2)on the expression of caspase-3 and the changes of ultra-structures in protoscolex cells of Echinococcus granulosus in vitro,the protoscolex cells were cultured in vitro with special RMPI-1640 medium in which glutamine was added.用原位末端脱氧核糖核苷酸转移酶标记技术(TUNEL法)检测原头节细胞凋亡情况,用过氧化物酶标记链霉卵白素(SP)染色半胱天冬氨酸蛋白酶-3(caspase-3)。
3)Caspase-3半胱天冬酶-3蛋白
1.Caspase-3 Expression in T-24 Bladder Carcinoma Cell after Irradiation by Holmium Laser;钬激光照射后人T-24膀胱癌细胞株中半胱天冬酶-3蛋白的表达及其意义
4)Caspase-3半胱天冬氨酸蛋白酶3
5)caspase3半胱天冬蛋白酶3
1.Objective:To observe the changes in caspase3 expression and apoptosis after liver ischemia/reperfusion(I/R),and to clarify whether propofol has a liver protection effect and the possible mechanism.目的:观察丙泊酚对大鼠肝脏缺血再灌注后细胞凋亡及半胱天冬蛋白酶3(caspase3)的表达的影响,探讨丙泊酚的肝脏保护作用及其可能机制。
6)caspase半胱天冬蛋白酶
1.Objective To study whether apoptin gene induces apoptosis in human melanoma cells A 375 via the activation caspase-8 and caspase-3.方法用含有apoptin基因的真核表达载体瞬间转染体外培养的人黑色素瘤细胞A 375;采用RT-PCR、DNA凝胶电泳、流式细胞仪分析检测A 375的细胞的凋亡;以比色法检测半胱天冬蛋白酶8(caspase-8)和caspase-3的相对活性。
2.Methods Cell viability was assessed by MTT assay;cell cycle distribution,apoptosis and mitochondrial membrane potential were measured by flow cytometry;the protein expressions of P-gp,caspase-3,caspase-8,caspase-9,p53,Bcl-xL and cytochrome c in the K562/DOX cells were evaluated by Western blot.方法以MTT法检测细胞活力,流式细胞仪检测细胞周期变化、凋亡率、线粒体膜电位的变化,Western blot检测P-gp及凋亡相关蛋白如半胱天冬蛋白酶-3、-8、-9、p53、Bcl-xL、cytochrome C的表达。
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天冬酸蛋白酶CAS: 78169-47-8 中文名称: 天冬酸蛋白酶 英文名称: Proteinase, aspartic;Proteinase,aspartic