1)Two-hybrid双杂交
1.New Type of Two-hybrid Systems in Protein-protein Interaction Studies;用于蛋白质间相互作用研究的新型双杂交系统
2.In this review, an improved two-hybrid system with technology of reporter protein fragment complementation and functional reconstitution is described.报告蛋白片段互补及功能重建技术是对传统的酵母双杂交技术的改进。
3.The concept, principles, advantages, disadvantages and applications of the current common drug screening technologies such as the two-hybrid technology, gene engineering technology, high-throughput screening, bioinformatics and so on were simply described in this paper.药物筛选的发展和新技术的出现是紧密相关的,文章对当前药物筛选中较常用的双杂交技术、基因工程技术、高通量筛选和生物信息学从概念、原理、优点、缺点和应用领域等方面作了简单的阐述,以期为相关的人员提供参考。
英文短句/例句
1.Construction of Bacterial Two-Hybrid System Library from the Young Panicle of Honglian Hybrid Rice红莲型杂交水稻细菌双杂交系统文库的构建
2.A cross in which each parent is the product of a single cross.It can be represented as AB?D, where A, B, C, and D are inbred lines.双杂交上代双方都是单杂交产物的杂交，可用AB?D表示，其中A、B、C和D是近交品系
3.A cross in which each parent is the product of a single cross. It can be represented as AB CD, where A, B, C, and D are inbred lines.双杂交上代双方都是单杂交产物的杂交，可用AB證D表示，其中A、B、C和D是近交品系
4.Potential Interacting Proteins for AMP-activated Protein Kinase α2 Revealed by Bacterial Two-Hybrid Screening;大肠杆菌双杂交筛选AMPKα2相互作用蛋白
5.To Fish the Human UROC-28 Binding Protein by the Yeast Two Hybrid System;酵母双杂交技术钓取人UROC28结合蛋白
6.Screening for Proteins Interacting with FOXP3 Gene by Yeast Two Hybrid System;酵母双杂交系统筛选FOXP3相互作用蛋白
7.Screening for Proteins Interacting with DND1 by Yeast Two-Hybrid System;酵母双杂交系统筛选DND1相互作用蛋白
8.Screening New Proteins Interacting with BACE1 by Yeast-two Hybrid;酵母双杂交筛选BACE1相互作用蛋白
9.Screening for Proteins Interacting with AtBAG5 by Yeast Two-hybrid System;酵母双杂交技术筛选AtBAG5的下游蛋白
10.Screening Interaction Protein of CTCF by Yeast Two Hybrid System;酵母双杂交系统筛选CTCF相互作用蛋白
11.Construction of Yeast Two-hybrid System of the bHLH-PAS Domain of hPER1;hPER1 bHLH-PAS结构域酵母双杂交系统的构建
12.Yeast Two-hybrid Analysis on Cryptochromes of Soybean;大豆隐花色素基因的酵母双杂交分析
13.Progress in the study of HIV-1 based on two-hybridization techniques双杂交技术在HIV-1研究中的应用进展
14.Screening New Binding-partners of Nogo-66 by Yeast Two-hybrid System;酵母双杂交筛选Nogo-66的结合分子及功能研究
15.Screening of β-lactamase Inhibitors by Yeast Two-hybrid System;利用酵母双杂交系统筛选β-内酰胺酶抑制剂
16.Screening Proteins to Interact with M1 by Yeast Two-hybrid System;酵母双杂交系统筛选与M1相互作用的蛋白质
17.Study on the Breeding of New Rapeseed Hybrid Zayou No.1 with Canola Quality;双低杂交油菜新品种“杂优1号”选育研究
18.mate a hybrid of the first generation with one of its parents.使第一代杂种与其双亲之一交配。
相关短句/例句
Diallel analysis双列杂交
1.Diallel analysis has been broadly used as an important tool of genetic analysis in classical quantitative genetics, which advanced the development of genetics studies and breeding practices.双列杂交分析是经典数量遗传学中应用最为广泛的一种重要的遗传分析方法,推动了遗传研究与育种实践的发展。
3)di-mon mating双单杂交
1.A wild strain and four types of sporaulated monokaryons of a cultivated strain Suxiang in Lentinula edodes were selected for the full compatiable di-mon mating, with one hybrid was obtained from each mating combination.选用香菇的野生株（Ｑ）与栽培株苏香的４种孢子单核体进行完全亲和双单杂交。
4)two-point-Crossover双点杂交
5)diallel cross双列杂交
1.The study on diallel cross of different shell color strains of Manila clam Ruditapes Philippinarum;不同壳色菲律宾蛤仔品系间的双列杂交
2.The diallel cross of Manila clam Ruditapes philippinarum of two shell shapes of Dalian population大连群体两种壳型菲律宾蛤仔的双列杂交
3.Six burley tobacco varieties and 15 combinations crossed by varieties using the 2nd method of Griffing s diallel cross were used to analyze combining ability and heritability of nicotine content,total nitrogen content,and nitrogen/nicotine ratio.利用6个白肋烟品种以及采用Griffing双列杂交第2种方法组配的15个杂交组合对构成白肋烟品质的烟碱、总氮含量及氮碱比3个数量性状的配合力和遗传力进行了分析,结果表明:对于这3个品质性状的一般配合力(GCA),6个白肋烟亲本间均存在极显著差异,组合间除上部叶氮碱比的特殊配合力(SCA)差异不显著外,其余性状的SCA均存在极显著差异,所有性状的GCA方差均大于SCA方差;KY8959和B67在品质性状上GCA效应表现较优,TN86和B37分别在降低中部叶烟碱和提高总氮含量上具有较好的GCA效应。
6)Diallel crossing双列杂交
1.The combining ability and population genetic parameters of ten main features belonging to eight maize inbred lines were studied by the design of complete diallel crossing method.采用完全双列杂交试验设计,对8个玉米自交系的10个主要性状的配合力和群体遗传参数进行分析。
延伸阅读
反向酵母双杂交系统确定了蛋白之间的相互作用后， 更重要的工作是研究其功能、结构及其作用的条件调节。鉴定在相互作用的一对蛋白中的任一蛋白突变对其相互作用的抑制作用， 不仅有助于探索相互作用的结构单位， 而且可作为研究体内功能的遗传学方法。这对于多个作用分子的蛋白就更为重要。 1996年vidalix x等建立了反向酵母双杂交系统(reverse two-hybrid system)， 提供了简便的鉴定阻断蛋白间相互作用的方法。反向酵母双杂交系统的关键是掺入一种表达产物对细胞生长有毒的报导基因， 用于监测蛋白间的相互作用。例如酵母ura3基因表达产物是尿嘧啶合成所必需的， 同时它又可催化5-氟乳清酸(5-foa)转化为有毒物质。vidal等构建了一酵母细胞株， 其ura3的表达由含gal4结合位点的启动子严密控制。此细胞株在缺乏尿嘧啶的培养基中培育需要gal4激活结构域(gad)和gal4 dna结合结构域(gbd)的融合蛋白的相互作用的表达。而在含5-foa的完全培养基中则受gad和gbd融合蛋白相互作用的抑制。因丝赏ü秆?-foa抗性克隆从随机突变库中鉴定阻断蛋白相互作用的突变体。反向双杂交系统可更有效地蛋白间作用的关键位点或起决定作用的个别氨基酸， 进而分析蛋白结构和功能的关系。此外此系统还可筛选能阻止某些蛋白间相互作用的肽或小分子物质用作临床治疗制剂。
