宏基因组文库构建,construction of metagenome
1)construction of metagenome宏基因组文库构建
英文短句/例句

1.Microbial Diversity Analysis and Metagenomic Library Construction of Red Soil in Jiangxi Provience;江西典型红壤区土壤微生物多样性分析与宏基因组文库构建
2.Investigation of Microbial Functional Ecology and Construction of Metagenomic Library for a Soil Sample from Native Forest森林红壤微生物的功能生态学分析及宏基因组文库构建
3.Construction of Metagenomic Library and Establishment of a Molecular Method for Characterization of Soil Microbial Community;土壤宏基因组文库构建与土壤微生物群落结构指示方法的初步建立
4.Construction of a Metagenomic Library of Uncultured Microorganisms from Compost and Cloning, Identification and Expression of Novel Xylanase Genes;堆肥未培养微生物的宏基因组文库构建及新的木聚糖酶基因的克隆、鉴定和表达
5.Bioactivity Evaluation of Culture Dependent Microorganisms & Construction and Screening of Soil Metagenome Library in Mangrove Environment;红树林可培养微生物活性评价和土壤宏基因组文库构建及生物活性筛选
6.The Construction of Metagenomic Library from the Alkalescence Environmental Samples and the Clone of Alkaline Protease Gene AP01;碱性土壤宏基因组文库的构建以及碱性蛋白酶AP01基因的克隆
7.Construction and Analysis of Rumen Bacterial Artificial Chromosome Library from a Holstein Dairy Cow Rumen Microflora;荷斯坦奶牛瘤胃微生物宏基因组BAC文库的构建与分析
8.DNA Extraction and Construction of a Metagenomic Fosmid Library of Alpine Meadow Soil from the Mila Mountains in Tibet,China西藏米拉山高寒草甸土壤微生物DNA提取及宏基因组Fosmid文库构建
9.Metagenomic fosmid library construction of Meloidogyne incognita associated bacteria and features analysis南方根结线虫伴生细菌宏基因组fosmid文库构建及其特征分析
10.Construction of Cosmid Library of Metagenome Derived from Compost Sample and the Improvement of DNA Isolation Techniques堆肥宏基因组柯斯质粒文库的构建和DNA提取技术的改进
11.Construction of Metagenomic Library from Soil and Screening of Clones with Antagonism to the Rice Blast Fungus(Magnaporthe grisea)土壤宏基因组文库的构建及拮抗稻瘟菌克隆子的筛选
12.The Construction of Metagenomic DNA Library from the Hypersaline Environmental Samples and the Clone of a New Amylase Gene;高盐极端环境土壤宏基因组文库的构建及一个新的淀粉酶基因的克隆
13.THE CONSTRUCTION OF GENOMIC LIBRARY FROM HUMAN GASTRIC CARCINOMA CELL LINE MGC80-3人胃癌MGC80—3细胞系基因组文库的构建
14.Baiji's Genomic DNA Library Construction and Application and the Investigation on Evolutionary Correlation of Mammalian C-value Variation;白鱀豚基因组DNA文库构建及应用
15.Staged Construction of Osteosacoma Genomic DNA Library;骨肉瘤基因组DNA文库的阶段性构建
16.The Construction and Preliminary Characterization of Rat Genomic Library;大鼠基因组文库的构建及其初步鉴定
17.Construction of Antarctic Notothenioid Fishes Dissostichus Mawsoni BAC Library;南极鱼Dissostichus mawsoni基因组BAC文库的构建
18.Construction of Genome BAC Library of Pseudomonas Pseudoalkaligenus YS1;假单胞菌YS1基因组BAC文库的构建
相关短句/例句

genome libraries construction基因组文库构建
3)metagenome library宏基因组文库
1.The large fragment metagenome library derived from natural mangrove soil over four seasons was successfully constructed by the optimized DNA extraction and electro elution purification method.采用改良及优化后的原位裂解法提取红树林土壤环境DNA,通过脉冲场电泳图谱显示所得DNA片段主要集中于23~50kbp范围,表明大片段DNA在总DNA中的比率明显提高;对改良后的原位裂解提取法与不同DNA纯化方法的组合进行比较分析,确定电洗脱为最佳纯化方法;运用改良后的原位裂解提取法与电洗脱纯化法联用实验方案,构建了4个季节天然红树林土壤Cosmid宏基因组文库。
2.This review discusses discovery of new polyketides via metagenome library technique.由于运用传统方法从自然界中直接筛选新型天然聚酮化合物的重现率很高,近年来出现了很多开发新型聚酮化合物的新方法,文章主要介绍通过环境宏基因组文库获得新聚酮类化合物的方法。
4)Metagenomic library宏基因组文库
1.Construction and diversity analysis of metagenomic library from antagonistic Magnaporthe grisea soil;稻瘟病抗性鉴定田土壤宏基因组文库构建及分析
2.A metagenomic library containing about 50,000 clones was constructed.从自制堆肥样品中提取未培养细菌的总DNA,用柯斯质粒pW EB∷TNC为载体构建宏基因组文库,对文库进行筛选获得表达木聚糖酶活性的克隆,再进行亚克隆、测序分析以及B lastx搜索G enB ank分析木聚糖酶基因。
3.Cells of uncultured microorganisms from self-made compost were isolated by differential centrifugation, metagenomic DNA of which was extracted, end-blunted and ligated with cosmid vector of pWEB::TNC to construct a metagenomic library containing about 50, 000 clones.通过差速离心从自制堆肥样品中分离未培养微生物细胞,提取其总DNA(metagenomic DNA),末端平头化后,用柯斯质粒pWEB::TNC为载体构建成了包含约5万个克隆的未培养微生物的宏基因组文库(metagenomic library),随机挑取其中的14个克隆提取质粒用BamHI酶切分析,结果显示所有的质粒都含有插入片段,最大为45kb,最小为25kb,平均大小为35kb,文库外源DNA总容量是1。
5)Fosmid metagenome libraryFosmid宏基因组文库
6)construction of genomic DNA library基因组DNA文库的构建
延伸阅读

基因组文库分子式:CAS号:性质:用限制性内切酶切割整个基因组DNA,可以得到大量基因组DNA片段,将这些片段与载体连接、转化细菌、形成克隆,这样一个克隆内的每个细胞的载体上都含有特定的基因组片段。这样的一套克隆就叫做基因组克隆,其中克隆的一套基因组片段就叫做基因组文库。