原生质体分离,protoplast isolation
1)protoplast isolation原生质体分离
1.The leaves of Cattleya hybrida were used as experiment materials and the influences of pretreatment time,enzyme combinations,digestion time and mannitol concentrations in the mixed enzyme solution on protoplast isolation from them were studied.试验以卡特兰叶片组织为材料,研究了预处理时间、酶液组合、酶解时间、酶液中甘露醇含量等不同因素对其原生质体分离的影响。
2.The results showed that the time of the protoplast isolation was prolonged for 2 hours when TSFEC had been cultured in media with Hygromycin,but the protoplast output and livability were little affected.以荔枝优良品种下番枝转gus和hpt基因悬浮细胞再生的松散性胚性愈伤组织(TSFEC)为试材,进行原生质体分离研究及初步培养。
3.incana seedlings,osmotic stabilizer,different combinations of enzyme mixture and digesting time,on protoplast isolation from the hypocotyls of M.以紫罗兰Matthiola incana无菌苗下胚轴为材料,研究了光照条件、无菌苗日龄、酶液渗透压、酶液组合及酶解时间对其原生质体分离效果。
英文短句/例句

1.Studies on Callus Induction and Protoplast Isolation on Ziziphus Jujuba Mill;枣愈伤组织诱导和原生质体分离研究
2.Advances on Isolation and Fusion of Plant Subprotoplasts植物亚原生质体分离及融合研究进展
3.Conditions Optimization of Arabidopsis Mesophyll Protoplast Isolation拟南芥叶肉原生质体分离条件的优化
4.Establishment of in Vitro Regeneration System and Protoplast Isolation of Orange-Heading Chinese Cabbage;橙色大白菜离体再生体系的建立及原生质体分离
5.Study on Regeneration System from Leaf in Vitro and Protoplast Isolation and Culture of Black Chokeberry;黑果腺肋花楸离体叶片再生体系建立与原生质体分离培养研究
6.Study on Protoplast Isolation and Electrofusion of Poplar;杨树原生质体分离及电融合技术体系的研究
7.The Skill of Hazel Mushroom and Pine Mushroom Protoplasm s Separation and Regeneration;蜜环菌和松蕈原生质体分离与再生的技术
8.Protoplast isolation and plant regeneration from leaves of Rhodiola sachalinensis高山红景天叶肉原生质体分离培养与植株再生
9.Protoplast Isolation of Callus in Echinacea augustifolia紫锥菊愈伤组织原生质体分离方法初探
10.Studies on the Protoplast Separation, Purification and Culture of Gladiolus;唐菖蒲原生质体分离、纯化及培养的研究
11.Establishment of Synchronous Suspension Culture and Isolation of Microprotoplasts in Citrus;柑橘同步化悬浮系建立及微原生质体分离
12.Studies on the Technique of the Isolation, Purification and Culture of Protoplast in Apple;苹果原生质体分离、纯化及培养技术研究
13.The Study on Primary Factors Influencing the Protoplast Culture and Isolation in Sugarcane;影响甘蔗原生质体分离、培养因素的研究
14.Study on Protoplast Isolation of Embryogenic Cell Suspensions of Musa acuminata cv.Mas(AA)贡蕉胚性悬浮细胞原生质体分离的研究
15.Studies on Callus Induction and Protoplast Isolation on Ziziphus jujuba Mill.cv.Dongzao冬枣花药愈伤组织的诱导及原生质体分离
16.1.Isolation and Culture of Protoplasts of Physcomitrium Sphaericum 2.Studies on RNAi System in Tobacco;1.立碗藓原生质体分离及培养的研究 2.烟草中RNAi体系的研究
17.Isolation of Mesophyll Protoplast from Arabidopsis Thaliana and the Setup of Transient Expression Assay System拟南芥叶肉原生质体分离及瞬时表达体系的建立
18.Influences of Seedling Age and Red Light on Protoplast Isolation and Culture of Sunflower苗龄与红光对向日葵原生质体分离和培养的影响
相关短句/例句

Isolation andregeneration of protoplast原生质体分离和再生
3)plasmolysis[英][pl?z'm?lisis][美][pl?z'mɑl?s?s]原生质分离
4)isolation and purification of protoplast原生质体分离和纯化
5)Dissociation of protoplasts原生质体游离
6)isolation of protoplast原生质休分离
延伸阅读

原生质体融合分子式:CAS号:性质:又称原生质体融合(protoplast fusion).使两个遗传性状不同的细胞发生融合,并进而获得杂种细胞的一项生物技术。是一种获得杂交细胞的很好方法。细胞融合不但可在不同种间进行,还可在亲缘关系较远的细胞中实现。所建立的杂交细胞可能含有来自双亲的细胞核和细胞质,亦可能丢失一方亲本的染色体而发生分离。杂交细胞的基因表达具有多样性。融合可以是自发的,但大多数可以诱导发生,某些病毒(如仙台病毒)、聚乙二醇、电场诱导等都可促进细胞融合。例如,淋巴细胞和骨髓瘤细胞融合形成的杂交瘤细胞,既具有淋巴细胞产生抗体的能力,又具有骨髓瘤细胞在体外可长期培养和无限增殖的特征,建立的杂交瘤细胞可永久提供稳定的多克隆或单克隆抗体。原生质体融合也是细胞融合的一种方法。