1)EST libraryEST文库
1.In order to determine the molecular mechanism of forming high quality tea-oil,the EST library technology was used to find out the main expressed genes controlling the fatty acid biosynthesis in camellia oleifera seeds.采用生物信息学方法,从随机测序而构建的油茶优良无性系湘林1号种子EST文库中初步鉴定了与茶油脂肪酸形成相关的大部分关键酶基因,并对其中几个直接控制不饱和脂肪酸的形成与转化的基因进行了相应的序列分析。
2.\'duiniantong\',and picked out randomly 3 107 clones for sequencing on the 5\'-end to construct the EST library,then performed homology comparison with nucleic acid NR database.为了研究与桐油合成相关的基因表达和分离基因,我们以发育中的对年桐种子为材料构建了cDNA文库,并随机挑取3107个克隆进行5\'-端测序构建表达序列标签EST文库,并进一步应用生物信息学方法在NCBI数据库中与其他植物核酸非冗余数据进行同源性比对,结果表明有2205个cDNA与其他植物种的基因有高度同源性,有482种基因得到初步鉴定,另有342个克隆可能为目前功能尚未知的基因。
3.The principle of EST library and its application in genetic map,isolation and identification new gene were simply introduced.综述了藻类EST文库的研究现状,并对EST的应用前景进行了展望。
英文短句/例句
1.Analysis on Expressed Sequence Tags(EST) and Research Progress of Algae EST Library表达序列标签(EST)分析及藻类EST文库的研究进展
2.Construction of EST Library, and Functional Notation and Database Management System of Part of ESTs of Maize;玉米EST文库的构建、部分EST功能注释及其数据库的管理系统
3.Construction of EST library of Vernicia fordii seeds and analysis of main expressed genes油桐种子EST文库构建及主要表达基因的分析
4.Establishment of Ests Database from Camellia Oleifera A. Seeds and Identification of Key Genes of Oil Synthetase;油茶种子EST文库构建及油脂合成关键酶基因的分离鉴定
5.Construction of cDNA Library of Bark in Ramie(Boehmeria Nivea(L.)Gaud) and EST Analysis;苎麻茎皮cDNA文库的构建及EST序列分析
6.Construction of cDNA Library and Analysis of the Expressed Sequenced Tags (ESTs) Characteristics of Dormant Seeds of Davidia involucrata休眠期珙桐种子cDNA文库构建及EST分析
7.EST-SSR Prediction of Heat Induced cDNA Library of Pinus teada火炬松热胁迫cDNA文库的EST-SSR预测
8.Construction of cDNA library for tender roots of tea plant(Camellia sinensis(L.) O.Kuntze) and analysis of its expressed sequence tags茶苗嫩根cDNA文库的构建和EST分析
9.cDNA Library Construction and EST Analysis of TcLr19 Induced by Puccinia Triticina;小麦叶锈菌诱导TcLr19cDNA文库的构建与EST分析
10.Construction of cDNA Library and Analysis on Expressed Sequence Tags of Genus of Lycoris Plant;石蒜属植物cDNA文库构建与表达序列标签（EST）分析
11.Construction of cDNA Library of Pichia Stipitis and Its ESTs Sequencing;树干毕赤酵母cDNA文库构建及EST序列测定
12.Construction of cDNA Library and Analysis of Expressed Sequence Tag in Haploid 10 of Laminaria Japonica;海带“单倍体十号”cDNA文库构建及EST序列分析
13.Construction of Normalized cDNA Library and Analysis on Expressed Sequence Tags of the Flower Development of CCRI 36;中棉所36花发育均一化cDNA文库构建及EST分析
14.Construction of Normalized Full-length cDNA Library of Brown Planthopper Larvae and Analysis of EST褐飞虱若虫均一化全长cDNA文库的构建及EST分析
15.Construction of Tender Shoots cDNA Library of Camellia Sinensis Cv. Ziyang 1 and Analysis of Expressed Sequence Tags紫阳1号茶树新梢cDNA文库构建及EST序列分析
16.Construction of Grains Development cDNA Library of Tartary Buckwheat and Analysis on EST from Partial Clones苦荞籽粒发育期cDNA文库的构建及部分EST分析
17.Construction of Full-Length cDNA Library from Floral Organ and EST Analysis in Guzmania擎天凤梨花器官全长cDNA文库的构建及EST分析
18.Construction of Full-length cDNA Library from Sugarcane Leaves and the Corresponding EST Analysis甘蔗叶片全长cDNA文库构建及EST序列分析
相关短句/例句
Differential display EST library差异显示EST文库
3)EST databaseEST数据库
1.In silico cloning based on EST sequence is a new method of gene cloning,using Arabidopsis thaliana homogentisate phytyltransferase cDNA sequence as a query probe to blast Glycine max EST database,a soybean HPT gene cDNA of 1 777 bp was obtained(GeneBank Accession,AY956421).采用基于EST的电子克隆方法,以拟南芥(Arabidopsis thaliana)的尿黑酸叶绿基转移酶基因(Homogentisate phytyltransferase,HPT)cDNA序列为信息探针,对大豆的EST数据库进行同源检索筛选,获得了1 777 bp长大豆HPT基因的cDNA序列(GenBank登录号为:AY956421),经RT-PCR扩增、分子克隆和序列分析验证,表明与电子克隆序列一致;该基因具有完整的开放阅读框架(ORF,173～1 408 bp),推测编码411个氨基酸的蛋白。
2.A query probe from the cDNA sequence of Arabidopsis thaliana pyridoxine biosynthesis protein was designed to blast Glycine max EST database according to the relative conservation of homologous genes and a soybean PDX gene cDNA of 1 280 bp was obtained(GenBank Accession,DQ139265).根据物种间同源基因相对保守的特点,以拟南芥(Arabidopsis thaliana)吡哆醇生物合成蛋白cDNA序列为信息探针,对大豆(Glycine max)EST数据库进行同源搜索和序列拼接,获得了1 280 bp长的大豆吡哆醇生物合成蛋白的基因序列(GenBank登陆号为DQ139265)。
3.A novel honeybee adenine nucleotide translocator gene (Am ant) cDNA (GenBank Accession, AY332626) was cloned by blasting the honeybee EST database with the fruit fly homologous ant gene cDNA sequence information as a querying probe.以果蝇腺苷酸转移载体基因cDNA序列为信息探针,对蜜蜂EST数据库进行同源检索筛选,克隆了蜜蜂腺苷酸转移载体基因(Am ant)的cDNA序列(GenBank登记号为AY332626),该基因全长1 251bp。
4)EST sequence databaseEST序列库
5)Endoscopic sphincterotomyEST
1.Objective To compare the therapeutic efficacy of endoscopic sphincterotomy(EST)with that of routine treatment in dealing with actue bilogenic pancreatitis(ABP).目的比较早期EST治疗ABP与常规治疗结果的差异。
6)Expressed Sequence TagEST
1.Construction of Intestinal cDNA Library and Analysis of Some Expressed Sequence Tags Sequencing of Ctenopharyngodon Idellus;草鱼肠道组织cDNA文库构建及部分ESTs分析
2.EST-SSR marker is a new kind of SSR markers which is developed from the ESTs(expressed sequence tags).小麦EST-SSR s是从小麦ESTs序列中开发的一种新型SSR标记。
3.Seven thousand seven hundred and fourteen contigs were obtained by assembling 13 007 expressed sequence tags (ESTs) of Chinese cabbage (Brassica campestris L.ssppekinensis)13007个EST序列进行拼接共得到7714个片段重叠群(contig),其中10。
延伸阅读
2-[[(2,4-dimethyl-3-cyclohexen-1-yl)methylene]amino]-benzoic aci methyl estCAS：68845-02-3分子式：C17H21NO2中文名称：2-[[(2,4-二甲基-3-环己烯-1-基)亚甲基]氨基]苯甲酸甲酯英文名称：Benzoic acid, 2-[[(2,4-dimethyl-3-cyclo hexen-1-yl)methylene]amino]-, methyl ester 2-[[(2,4-dimethyl-3-cyclohexen-1-yl)methylene]amino]-benzoic aci methyl est
