K562/Dox细胞,K562/Dox cell
1)K562/Dox cellK562/Dox细胞
1.OBJECTIVE To develop a method using HPLC with fluorescence detector for determining the Doxorubicin concentrations in K562/DOX cell nuclei.方法分离K562/DOX细胞核,多柔比星浓度测定采用Diamond C18色谱柱,甲醇-水-醋酸(50∶50∶0。
2.Methods To detect the survival rate of K562/DOX cell,which was dealt with ADM alone or with both ADM and Chinese Magnolivine Fruit Extraction.方法MTT法检测单独应用阿霉素或阿霉素与五味子提取物联合作用的K562/DOX细胞的生存率;RT-PCR检测MDR1、MRP1 mRNA表达;Westernblotting检测MDR1蛋白的表达。
2)K562/DOX cell lineK562/DOX细胞株
3)MCF-7/DOX cellMCF-7/DOX细胞
1.RT-PCR assay and immunity histochemistry assay were used to determine the expression level of mdr1 gene and P-gp in MCF-7/DOX cells to elucidate resistant character of MCF-7/DOX cells.首先以RT-PCR和免疫组化方法分别从RNA和蛋白水平检测MCF-7/DOX细胞P-gp表达情况,以明确MCF-7/DOX细胞的耐药特征;然后采用MTT法检测异汉防己碱的内在细胞毒性及其对阿霉素(DOX)的增敏作用,并以RF(reversal fold)值评价其逆转效果;同时应用流式细胞仪(FCM)对细胞内DOX的蓄积量进行了分析;再以免疫组化方法检测异汉防己碱对MCF-7/DOX细胞P-gp表达水平的影响;最后采用罗丹明蓄积和外排试验检测了异汉防己碱对P-gp功能的影响。
4)K562 cellK562细胞
1.An experimental study on reconstruction of retrovirus vector in human leukemia K562 cell xenograft tumor of nude Mice;重组逆转录病毒RV-40AS对K562细胞裸鼠移植瘤的实验研究
2.Effect of total saponins of panax ginseng on the expression of erythropoietin receptor in K562 cells;人参总皂苷对K562细胞红细胞生成素受体表达的影响
3.Study on apoptosis of K562 cells staining with Annexin V/PI induced by high-voltage pulse electric field;Annexin V/PI双标记检测高电压脉冲电场诱导K562细胞凋亡的研究
英文短句/例句

1.Study of Function of OAZ Gene during Differentiation of K562 Cells;OAZ基因在K562细胞分化中的功能研究
2.Mechanism of Simvastatin-Induced Apoptosis of K562 Cells;辛伐他汀诱导K562细胞凋亡机制的研究
3.Study on the Resistant Mechanisms of K562 Cells Induced by STI571;STI571诱导K562细胞耐药机制的研究
4.Study of the RAS Gene Function in the Apoptosis of K562 Cell Line;RAS基因在K562细胞凋亡中的作用研究
5.The Study about the Function of Cyclin E in K562 Cells with Methods of RNAi and DNA Microarray;Cyclin E基因在K562细胞周期调控中的作用
6.Expression of OD Protein and Effects of OD on the K562 Cell;OD蛋白的表达及对K562细胞的作用
7.The Effects of STAT5 Decoy ODNS ON K562;STAT5诱骗核苷酸对白血病K562细胞的影响
8.Differentiation Phenotypes of K562 Cells Induced by Exogenous Wnt5a;外源性Wnt5a诱导K562细胞定向分化的研究
9.Influence of daunorubicin on apoptosis factor of K562 cells柔红霉素对K562细胞凋亡的影响
10.Low power microwave irradiation inhibits proliferation of K562 cells低功率微波对K562细胞增殖的影响
11.Enhancement effects of hypoxia on invasion and metastasis of K562 cells低氧对K562细胞侵袭转移的增强作用
12.Effect of total saponins of panax ginseng on expression of STAT5 in K562 cells人参总皂苷对K562细胞STAT5表达的影响
13.Effect of Silencing Bmi-1 by RNA Interference on Function of K562 Cell LineBmi-1沉默对K562细胞功能的影响
14.Effect of Berberine on the Differentiation and Apoptosis of K562 Cell Line小檗碱对K562细胞分化及凋亡的影响
15.Effects of static magnetic fields of different intensities on K562 leukemia cells不同强度的静磁场对K562细胞的作用
16.Regulatory effects of PTEN gene on mTOR signaling pathway in K562 cell linePTEN对K562细胞mTOR调控的研究
17.Preventing contamination in laboratory culture of k562 cellK562细胞实验室培养污染的防控
18.The Effect and Molecular Mechanism for Artesunate on the CD34~+ Cell Separated from K562 Cell Line;青蒿琥酯对K562细胞系CD34~+细胞的作用及分子机制
相关短句/例句

K562/DOX cell lineK562/DOX细胞株
3)MCF-7/DOX cellMCF-7/DOX细胞
1.RT-PCR assay and immunity histochemistry assay were used to determine the expression level of mdr1 gene and P-gp in MCF-7/DOX cells to elucidate resistant character of MCF-7/DOX cells.首先以RT-PCR和免疫组化方法分别从RNA和蛋白水平检测MCF-7/DOX细胞P-gp表达情况,以明确MCF-7/DOX细胞的耐药特征;然后采用MTT法检测异汉防己碱的内在细胞毒性及其对阿霉素(DOX)的增敏作用,并以RF(reversal fold)值评价其逆转效果;同时应用流式细胞仪(FCM)对细胞内DOX的蓄积量进行了分析;再以免疫组化方法检测异汉防己碱对MCF-7/DOX细胞P-gp表达水平的影响;最后采用罗丹明蓄积和外排试验检测了异汉防己碱对P-gp功能的影响。
4)K562 cellK562细胞
1.An experimental study on reconstruction of retrovirus vector in human leukemia K562 cell xenograft tumor of nude Mice;重组逆转录病毒RV-40AS对K562细胞裸鼠移植瘤的实验研究
2.Effect of total saponins of panax ginseng on the expression of erythropoietin receptor in K562 cells;人参总皂苷对K562细胞红细胞生成素受体表达的影响
3.Study on apoptosis of K562 cells staining with Annexin V/PI induced by high-voltage pulse electric field;Annexin V/PI双标记检测高电压脉冲电场诱导K562细胞凋亡的研究
5)K562 cell lineK562细胞株
1.Cytotoxicity of cord blood monocyte-macrophage activated by cytokines against K562 cell line;细胞因子激活的脐血单核细胞对K562细胞株杀伤作用的观察
2.Aim To explore the effect of 4-amino-2-trifluoromethyl-phenyl retinate(ATPR)on proliferation,differentiation activity in K562 cell line,and to research the mechanisms.目的本研究探讨新型维甲酸衍生物4-氨基-2-三氟甲基苯基维甲酸酯(4-amino-2-trifluoromethyl-phenyl retinate,ATPR)对K562细胞株的抑制增殖和诱导分化活性并对其机制进行研究。
6)K562 cellsk562细胞
1.Differentiation of K562 cells into DC-like cells induced by the combination of calcium ionorphore with rhGM-CSF and rhIL-4;钙离子载体促进K562细胞诱导分化成DC样细胞的研究
2.Anti-ABL tyrosine kinase intrabody inhibits growth of K562 cells in vitro;抗ABL酪氨酸激酶区胞内抗体抑制K562细胞体外生长
3.Effects of the main extracts of Astragalus membranaceus on inducing the erythroid differentiation of K562 cells;黄芪不同提取物对K562细胞向红系分化的诱导作用研究
延伸阅读

Czapek-Dox medium分子式:CAS号:性质:又称查氏培养基。实验室常用的一种培养各种腐生真菌和土壤细菌等的合成培养基。主要成分为蔗糖、硝酸钠、磷酸氢二钾、硫酸镁、氯化钾和硫酸亚铁等,若加入凝固剂琼脂,就可以配制成固体培养基。