1)CXCL10-loop3-EGF fusion proteinCXCL10-loop3-EGF融合蛋白
1.AIM:To evaluate the implication of CXCL10-loop3-EGF fusion protein for the activities of targeting tumor and anti-angiopoiesis.方法:运用RT-PCR从经IFN-γ刺激活化的PBMC中扩增人CXCL10基因,运用重叠延伸PCR法扩增CXCL10-loop3-EGF融合基因,并将其与载体pTG19-T连接、转化大肠杆菌DH5α、筛选阳性克隆,CXCL10-loop3-EGF融合基因与载体pET32a(+)连接、转化大肠杆菌Origami B(DE3),诱导可溶性表达重组his-CXCL10-loop3-EGF融合蛋白,并经镍柱亲和层析、酶切、超滤及透析等方法获得纯化的重组CXCL10-loop3-EGF融合蛋白。
2)EGF-IL-18 fusion proteinEGF-IL-18融合蛋白
3)fusion protein融合蛋白
1.Molecular design and characterization of recombinant fusion protein of human IFNα_(2a)-THYα_1;重组人干扰素α_(2a)和胸腺肽α_1融合蛋白的分子设计及其基因表达产物的鉴定
2.Expression and purification of glycerol dehydratase β-subunit in E.coli as fusion protein;甘油脱水酶β亚基融合蛋白在大肠杆菌中的表达和纯化
3.Pro-apoptotic effect of recombinant anti-HER2 fusion protein ScFv/tBid gene on osteosarcoma E10 cells;重组抗HER2融合蛋白基因ScFv/tBid对骨肉瘤E10细胞的促凋亡作用
英文短句/例句
1.antibody fusion protein抗体融合蛋白(质)
2.Study of Recombinant Human Serum Albumin-Interferon-α 2b Fusion Protein;重组人血清白蛋白—干扰素α 2b融合蛋白的研究
3.Expression and Identification of the Endostatin-HSA Fusion Protein内皮抑素—人血清白蛋白融合蛋白的表达及鉴定
4.Expression and purification of fusion protein containing the segment of glycoprotein G of RSV and the serum albumin-binding region of streptococcal protein G(BB)SPG血清白蛋白结合区和RSV的G蛋白片段融合蛋白的构建和表达
5.Immunogenicity of ETEC K99 Protein and Fusion Protein K88ac-STII;重组ETEC K99蛋白和K88ac-STII融合蛋白的免疫原性研究
6.Cloning and Expression of the Genes Encoding Salmine in Escherichia coli鲑精蛋白基因克隆与融合蛋白表达系统的构建
7.Construction of a recombinant plasmid expressing HBV core antigen and A3C fusion proteinHBV核心蛋白与A3C融合蛋白HBV载体质粒的构建
8.Expression and Fermentation of the Fusion Protein of Human Serum Album/Interleukin-2;人血清白蛋白/人白介素-2融合蛋白的表达与发酵
9.Fermentation and Purification of the Fusion Protein of Interleukin-2/Human Serum Album人白介素-2/人血清白蛋白融合蛋白的发酵与纯化研究
10.Functional Study of HSP65-PSA Multiple-Epitopes Fusion Protein Vaccine;HSP65-PSA多表位融合蛋白疫苗的功能研究
11.Determination of Expression and Function on m_4 AChR-Gilα Fusion Protein;m_4AchR-Gilα融合蛋白的表达及功能的研究
12.Prokaryotic Expression and Purification of Fusion Protein of PTD-SH2;PTD-SH2融合蛋白的原核表达和纯化
13.The Purification of sflk1-IFN-γ Fusion Protein;sflk1-IFN-γ融合蛋白的分离与纯化
14.Expression and Analysis of Biological Activity of PTD-hBDNF;PTD-hBDNF融合蛋白的表达与生物活性测定
15.Construction and Expression of HPV16 CTL Multi-Epitope Fusion Protein;HPV16型CTL多表位融合蛋白的构建及表达
16.The Construction, Expression and Purification of Recombinant IL-4 Fusion Protein;重组IL-4融合蛋白的构建、表达及纯化
17.Expression and Secretion of the Recombinant UKAV and Its Biological Function;UKAV融合蛋白的表达及其生物活性研究
18.Expression of TrxA-EstB1 Fusion Protein and Its Purification, Characterization of Enzyme Activity;TrxA-EstB1融合蛋白的表达、纯化与活性分析
相关短句/例句
EGF-IL-18 fusion proteinEGF-IL-18融合蛋白
3)fusion protein融合蛋白
1.Molecular design and characterization of recombinant fusion protein of human IFNα_(2a)-THYα_1;重组人干扰素α_(2a)和胸腺肽α_1融合蛋白的分子设计及其基因表达产物的鉴定
2.Expression and purification of glycerol dehydratase β-subunit in E.coli as fusion protein;甘油脱水酶β亚基融合蛋白在大肠杆菌中的表达和纯化
3.Pro-apoptotic effect of recombinant anti-HER2 fusion protein ScFv/tBid gene on osteosarcoma E10 cells;重组抗HER2融合蛋白基因ScFv/tBid对骨肉瘤E10细胞的促凋亡作用
4)Fusion proteins融合蛋白
1.Separation and purification of recombinant fusion proteins of IFNα_(2b)-THYα_1;重组人干扰素α_(2b)和胸腺肽α_1融合蛋白的分离纯化
2.After induction by isopropyl β-D-1-thiogalactopyranoside(IPTG),the observed yield of crude fusion proteins was up to 260 mg/L.将构建的含有胸腺肽α1融合蛋白的编码基因插入表达质粒pET28a(+)中,并在大肠杆菌BL21中进行表达,该菌经IPTG诱导融合蛋白高表达后,离心收集菌体,超声波破碎,包含体裂解,目标蛋白质的体外变性复性后,经DEAE-Sepharose Fast Flow阴离子交换层析纯化,每升菌液中可获得260 mg融合蛋白。
3.Objective: To express the fusion proteins containing VP3 and Tat-PTD (protein transduction domain) proteins, and then purify and dialysis on this fusion proteins.目的:原核表达VP3与Tat-PTD(protein transduction domain)的融合蛋白,并纯化此融合蛋白,观察Tat-PTD介导VP3进入胃癌细胞SGC7901的透膜效应及VP3在肿瘤细胞中的定位,检测融合蛋白诱导细胞凋亡情况。
5)recombinant protein融合蛋白
1.Objective To explore the reasons for the low intracellular transduction efficiency of a previously constructed His-TAT-Flag recombinant protein and establish a more efficient transduction system.目的探索本室构建的pET14b-His-TAT-Flag重组载体表达的融合蛋白在细胞中转导效率低的原因,建立高效的细胞内转导系统。
2.The purified recombinant protein was analyzed by SDS-PAGE and Western blotting,the results showed that the CP fragment was highly expressed.重组蛋白经过Ni+-NTA纯化后,SDS-PAGE和Western blotting分析结果表明,TRSV-CP基因在大肠杆菌中获得了高效表达,产生的融合蛋白分子质量约为34 ku,并具有免疫学活性。
3.A recombinant protein with about 29kD was expressed stably in the form of insoluble inclusion bodies after IPTG induction.PCR扩增拟南芥MnSODcDNA的保守区段,构建pET-SOD重组质粒,转化大肠杆菌JM109穴DE3雪,IPTG诱导融合蛋白高效表达;经检测表达产物占菌体总蛋白的69%,且以不溶的包涵体形式存在;表达产物变性后经Ni-NTASuperflow亲和柱分离纯化,得到相对分子质量约为29000的PAGE纯蛋白。
6)fused protein融合蛋白
1.Expression of fused protein A-green fluorescent protein (PA-GFP);蛋白A-绿色荧光蛋白融合蛋白的构建与表达
2.AIM: To construct the prokaryotic recombinant plasmid to express HBV truncated C gene with preS1 gene and to acquire and purify the fused protein for antigenic analysis.目的:构建乙型肝炎病毒(HBV)截短C基因和前S1基因重组原核表达质粒,获得融合蛋白的表达并进行抗原性分析。
3.A method for the purification of expressed maltose binding protein fused protein by using potato starch instead of crosslinked amylose have been developed.用马铃薯淀粉柱可以直接分离麦芽糖结合蛋白乳酸脱氢酶辅酶结合结构域融合蛋白,并得到满意的结果。
延伸阅读
[3-(aminosulfonyl)-4-chloro-N-(2.3-dihydro-2-methyl-1H-indol-1-yl)benzamide]分子式:C16H16ClN3O3S分子量:365.5CAS号:26807-65-8性质:暂无制备方法:暂无用途:用于轻、中度原发性高血压。
