荧光报告基因,Fluorescence reporter gene英语短句,例句大全

荧光报告基因,Fluorescence reporter gene
1)Fluorescence reporter gene荧光报告基因
2)luciferase reporter gene荧光素酶报告基因
1.These DNA fragments were inserted into the pGL3-Basic which was the vector of luciferase reporter gene.方法提取人内皮细胞基因组DNA,设计引物克隆EOLA1基因上游不同长度的基因片段,插入到含荧光素酶报告基因的载体pGL3-Basic中,经限制性内切酶酶切鉴定、测序。
2.[Method] Clonging the promoters of CYP3A4 and CYP2B6 which contained the elements that hPXR, a kind of nuclear receptor, can recognize and bind, and inserting the trans-element to the upstream of firefly luciferase reporter gene of the pGL4.方法利用双荧光素酶报告基因系统,将包含hPXR蛋白识别和结合调控元件的CYP3A4和2B6启动子序列插入报告基因上游,将表达载体和报告载体共转染HepG2细胞,用含有利福平或DMSO培养基培养48h后裂解进行双荧光素酶活性检测。
3.Amplified apolipoprotein A-I(apoA-I) promoter gene(376 bp) containing essential transcription regulatory elements from human genomic DNA was fused upstream to the luciferase reporter gene and neomycin resistance gene in the constructed pGL3B-neo vector.PCR扩增含有apoA-I基因的转录调控序列的启动子片断(376 bp),克隆入含有荧光素酶报告基因和新霉素抗性基因的pGL3B-neo载体中,构成受apoA-I启动子调控的重组报告基因质粒pGL3B-neo::apo,稳定转染人肝癌细胞株HepG2。

英文短句/例句

1.Construction of Dual-Luciferase reporter plasmid assay system for human NOX1 geneNOX1基因荧光素酶报告基因系统的建立
2.Construction and identification of luciferase reporter gene vector containing HMGB1 promoterHMGB1启动子荧光素酶报告基因的构建及鉴定
3.Creation and Identification of Luciferase Reporter Gene Containing Human Bax Promotor人Bax启动子荧光素酶报告基因的构建和鉴定
4.Construction and identification of human Puma promotor luciferase report gene vector人Puma启动子荧光素酶报告基因的构建和鉴定
5.Construction of luciferase reporter gene plasmid containing hBD-2 and mutant correlated with Crohn's diseaseβ-防御素-2荧光素酶报告基因载体及克罗恩突变体的构建
6.Construction of Recombinant Retroviral Vector Containing Firefly Luciferase Reporter Gene萤火虫荧光素酶报告基因重组逆转录病毒载体的构建及鉴定
7.Construction and analysis of the luciferase reporter vectors of human SMYD3 gene promoter人SMYD3基因启动子荧光素酶报告载体的构建与分析
8.The Construction of Luciferase Reporter Gene Vectors Containing Different Haplotypes DNA of Human PLUNC Gene Promoter RegionPLUNC基因启动子区荧光素酶报告载体的构建与鉴定
9.Establishment of a cellular assay based on reporter gene for identification of strogenic compounds建立基于荧光素报告基因系统检测雌激素样物质方法的研究
10.An efficient method for screening effective siRNAs using dual-luciferase reporter assay system利用双萤光素酶报告基因系统筛选有效的siRNA
11.Construction and expression of red fluorescent protein reporter gene vector for human SOD1人铜锌超氧化物歧化酶基因启动子驱动的红色荧光蛋白报告基因载体的构建及表达
12.Molecular cloning and constructing luciferase report gene vector for the gene promoter of CCDC72,a novel modulator for hair follicule developement调控毛囊生长CCDC72基因启动子克隆及萤光素酶报告基因载体的构建
13.Construction and expression of red fluorescent protein reporter gene vector containing human eNOS promoter人内皮细胞一氧化氮合酶红色荧光蛋白报告基因载体的构建与表达
14.Construction and identification of a luciferase reporter vector containing human CXCR4 promoter人CXCR4基因启动子萤光素酶报告载体的构建及鉴定
15.Optimization of the expression of luc gene in E. coli荧光素酶基因luc在大肠杆菌中表达条件优化
16.Viviperception of Transgenic Human Embryonic Stem Cells Labeled by Luciferase荧光素酶标记转基因人胚胎干细胞的活体观察
17.Gene Imaging by Luciferase in Vitro Detecing Therapeutic Effect of hTRAIL for Lung Cancer A549 Cells;荧光素酶体外监测hTRAIL基因治疗肺癌A549细胞的基因成像研究
18.Construction and functional identification of red fluorescent protein reporter plasmid for human catalase gene人CAT启动子红色荧光蛋白报告基因载体的构建及功能鉴定

相关短句/例句

luciferase reporter gene荧光素酶报告基因
1.These DNA fragments were inserted into the pGL3-Basic which was the vector of luciferase reporter gene.方法提取人内皮细胞基因组DNA,设计引物克隆EOLA1基因上游不同长度的基因片段,插入到含荧光素酶报告基因的载体pGL3-Basic中,经限制性内切酶酶切鉴定、测序。
2.[Method] Clonging the promoters of CYP3A4 and CYP2B6 which contained the elements that hPXR, a kind of nuclear receptor, can recognize and bind, and inserting the trans-element to the upstream of firefly luciferase reporter gene of the pGL4.方法利用双荧光素酶报告基因系统,将包含hPXR蛋白识别和结合调控元件的CYP3A4和2B6启动子序列插入报告基因上游,将表达载体和报告载体共转染HepG2细胞,用含有利福平或DMSO培养基培养48h后裂解进行双荧光素酶活性检测。
3.Amplified apolipoprotein A-I(apoA-I) promoter gene(376 bp) containing essential transcription regulatory elements from human genomic DNA was fused upstream to the luciferase reporter gene and neomycin resistance gene in the constructed pGL3B-neo vector.PCR扩增含有apoA-I基因的转录调控序列的启动子片断(376 bp),克隆入含有荧光素酶报告基因和新霉素抗性基因的pGL3B-neo载体中,构成受apoA-I启动子调控的重组报告基因质粒pGL3B-neo::apo,稳定转染人肝癌细胞株HepG2。
3)luciferase report gene荧光素酶报告基因
1.Methods The +495bp～+584bp of SCN5A promoter was amplified by polymerase chain reaction(PCR)and recombinated into pGL3-promoter luciferase report gene vector and transient transfected HEK293 cell and H9C2 cell.方法应用聚合酶链反应扩增大鼠心肌SCN5A基因启动区+495bp~+584bp片段,与荧光素酶报告基因载体pGL3-promoter重组,以pGL3-promoter空载体为对照,瞬时转染人胚肾母细胞(HEK293)和小鼠胚胎心肌细胞(H9C2),检测荧光素酶活性。
2.coli chromosome,the luciferase report gene was knocked in lacZ site of chromosome lac operon by using Red recombination system and selection-counterselection kan/sacB technology.为了应用Red重组工程技术实现外源基因在大肠杆菌染色体上的表述,寻找染色体上外源蛋白的稳定高效表达位点,使用Red重组工程系统和kan/sacB无痕迹修饰技术,将易于定量分析的荧光素酶报告基因替换DY330染色体lac操纵子中的lacZ基因。
4)dual-luciferase report gene双荧光素酶报告基因
5)Jun-2 luciferase report geneJun-2荧光素酶报告基因
6)Green fluorescent protein reporter gene绿色荧光蛋白报告基因