塞内卡病毒A免疫原性组合物及其方法与流程
序列表
本申请含有根据37c.f.r.1.821–1.825的序列表。本申请所附的序列表通过引用整体并入本文。
发明背景
a.技术领域
本发明涉及塞内卡谷病毒a或塞内卡病毒a(sva)，及其作为免疫原性组合物或疫苗以治疗受塞内卡病毒a感染的动物的用途。
b.相关技术描述
sva是微小核糖核酸病毒科(picornaviridae)内的无包膜、单链、正义rna病毒。口蹄疫病毒(fmdv)和猪水泡病病毒(svda)也是微小核糖核酸病毒科的成员。
该病毒最初作为来自细胞培养基的污染物被发现(参见hales，l.m.等人，completegenomesequenceanalysisofsenecavalleyvirus-001，anoveloncolyticpicornavirus.jgenvirol，2008.89(pt5)：第1265-75页，通过引用整体并入本文)。然而，已在猪、牛、鼠和人中检测到针对该病毒的中和抗体(参见knowles，n.j.等人，epidemiologyofsenecavalleyvirus:identificationandcharacterizationofisolatesfrompigsintheunitedstates，inthenorthernlightseuropic2006-14thmeetingoftheeuropeanstudygrouponthemolecularbiologyofpicornaviruses.2006:saairselka，inari，芬兰，通过引用整体并入本文)。感染后报告的临床体征包括口鼻部和冠状带上的水泡病变，急性跛行，冠状带溃疡和蹄脱落(singh，k.等人，senecavalleyvirusandvesicularlesionsinapigwithidiopathicvesiculardisease.jvetscitechnol，2012.3(6)，以及pasma，t.，s.davidson和s.l.shaw，idiopathicvesiculardiseaseinswineinmanitoba.cvj，2008.49:第84-85页，两者均通过引用整体并入本文)。在2016年，当细胞培养繁殖的sva分离株用于接种常规动物，并且在接种后四天观察到水泡病变时，koch的假设得以实现(montiel，n.等人，vesiculardiseasein9-week-oldpigsexperimentallyinfectedwithsenecavirusa.emerginfectdis，2016.22(7)：第1246-8页，通过引用整体并入本文)。
us8,039,606描述了塞内卡谷病毒治疗肿瘤的用途。然而，这种塞尼卡谷病毒不同于本发明的sva。
在美国、加拿大、澳大利亚、意大利、新西兰和巴西，已存在无法解释的sva病例增加。进一步地，由于临床体征与fmdv的相似性，这种病毒引起了养猪业的兴趣。兽医生物制品中心(centerforveterinarybiologics)(cvb)通知16-03确认，cvb对许可用于sva病毒的生物制品和/或预防剂感兴趣。
技术实现要素：
本发明提供了克服本领域缺陷的免疫原性组合物、疫苗和相关方法。所述组合物和方法提供了免疫原性组合物，其包括sva的无包膜(+)单链rna病毒的灭活/杀死的和/或重组形式。特别地，本申请提供了用于在猪中生成免疫应答的疫苗，用于保护免受与塞内卡病毒a相关的疾病。本文的塞内卡病毒a分离株nac#20150909(seqidno：1、seqidno：2和/或seqidno：3)从由5月龄的猪收集的水泡液中分离，所述猪显示出在口鼻部和冠状带上的水泡病变。
本发明的免疫原性组合物和疫苗包含seqidno：1、seqidno：2和/或seqidno：3。
本发明的示例性组合物包含seqidno：3的多肽序列或其对sva具有免疫反应性的片段。
本发明的免疫原性组合物和疫苗包含sva抗原，在一个非限制性实例中，所述sva抗原经由重组杆状病毒在昆虫细胞中表达，所述重组杆状病毒表达经修饰的svap1、2a，部分2b和3b、以及3c蛋白酶，例如编码氨基酸序列(seqidno：19)的经修饰的sva核酸序列(seqidno：18)，并且通常还包括佐剂。疫苗还可以包括其它组分，例如防腐剂、稳定剂和针对其它猪病原体的抗原。
适用于本发明的优选的p1-2a-p3核酸序列是编码p1-2a-p3多肽的多核苷酸，所述多核苷酸与seqidno：18、20、22、24、32和33具有至少80％、81％、82％、83％、84％、85％、86％、87％、88％或89％，更优选至少90％、91％、92％、93％或94％，且最优选至少95％、96％、96.1％、96.2％、96.3％、96.4％、96.5％、96.6％、96.7％、96.8％、96.9％、97％、97.1％、97.2％、97.3％、97.4％、97.5％、97.6％、97.7％、97.8％、97.9％、98％、98.1％、98.2％、98.3％、98.4％、98.5％、98.6％、98.7％、98.8％、98.9％、99％、99.1％、99.2％、99.3％、99.4％、99.5％、99.6％、99.7％、99.8％、99.9％的序列相同性。“如本文使用的，应特别理解术语“与seqidno：x的序列相同性”或“相同seqidno：x”分别等价于术语“在seqidno：x的长度上与seqidno：x的序列相同性”或“在seqidno：x的长度上与seqidno：x的序列相同”，分别地，其中在此上下文中，“x”是选自18、20、22、24、32和33”的任何整数。
适用于本发明的优选p1-2a-p3多肽是具有seqidno:19、21、23、25、27和29中所示的序列的多肽，与seqidno:19、21、23、25、27和29具有至少80％的同源性，例如与seqidno:19、21、23、25、27和29具有至少85％的同源性，例如与seqidno:19、21、23、25、27和29具有至少85％的同源性，例如与seqidno:19、21、23、25、27和29具有至少90％的同源性，例如与seqidno:19、21、23、25、27和29具有至少95％、至少98％或至少99％的同源性。
在另一个方面，本发明提供了编码一种或多种多肽、抗体构建体或抗体缀合物的核酸序列。编码多肽的基因序列包含与seqidno：1或seqidno：2的序列至少95％、90％、85％或甚至80％同源和/或相同的核酸序列，或其编码对sva具有免疫反应性的多肽的片段。本发明的示例性核酸序列包括seqidno：1、seqidno：2及其编码对sva具有免疫反应性的多肽的片段的任何一种序列。
此外，如本文使用的，本发明的多肽包括但不限于包含以下的多肽：
i)包含seqidno：3的氨基酸序列的多肽；
ii)与i)的多肽至少80％同源和/或相同的多肽；
iii)i)和/或ii)的多肽的片段；
iv)i)或ii)的多肽；
v)iii)或iv)的片段，其包含seqidno：3的序列中包括的至少5个，优选8个，更优选10个，甚至更优选15个邻接氨基酸；
vi)由包含seqidno：1或2的序列的多核苷酸编码的多肽；
vii)由与vi)的多核苷酸至少80％同源或相同的多核苷酸编码的多肽；
viii)由多核苷酸编码的蛋白质片段，所述多核苷酸包含seqidno：1或seqidno：2的序列中包括的至少15个，优选24个，更优选30个，甚至更优选45个邻接核苷酸。
包含如本文定义的至少一种或多种sva多肽的本发明的免疫原性组合物可以进一步包含生理学可接受的媒介物，例如药学或兽医学可接受的载体、佐剂或其组合。
本文提供的任何sva多肽、或者包含本文提供的这些sva多肽中的一种或多种的任何免疫原性组合物可以用作药物，优选用作疫苗或免疫原性组合物，最优选用于对象针对sva感染的预防或治疗。
本领域技术人员将理解，本文使用的组合物可以掺入已知的可注射的、生理学可接受的无菌溶液。为了制备用于肠胃外注射或输注的即用溶液，等渗水溶液，例如盐水或血浆蛋白溶液是可容易获得的。另外，本发明的免疫原性和疫苗组合物可以包括兽医学可接受的载体、稀释剂、等渗剂、稳定剂或佐剂。
本发明的方法包括但不限于在对象中引发针对sva感染的免疫应答的方法，其包括向对象施用包含如本文定义的一种或多种sva多肽的免疫原性组合物的步骤。本发明的组合物可以用于治疗或可替代地用于预防sva感染。优选地，这种免疫应答减少与sva血清型感染相关或由其引起的一种或多种临床症状的发生率或严重性。
在本文中，合适的对象和需要对其施用本发明的组合物的对象包括需要预防或治疗病毒相关感染、疾病或状况的动物。其中通过使用本发明的组合物或方法刺激免疫应答的动物包括家畜，例如猪、牛、山羊和绵羊。优选动物包括猪科动物、鼠科动物、马科动物、兔形目动物和牛科动物。最优选地，在猪中刺激免疫应答。
本发明还提供了减少与sva感染相关或由其引起的一种或多种临床体征的发生率或严重性的方法，其包括施用本发明免疫原性组合物的步骤，所述免疫原性组合物包含如本文提供的一种或多种sva肽以及优选包含载体分子，使得相对于仍未接受如随同提供的免疫原性组合物的对象，sva感染的临床体征的发生率或严重性减少至少10％，优选至少20％，甚至更优选至少30％，甚至更优选至少50％，甚至更优选至少70％，最优选至少100％。
此类临床体征包括水疱病，例如位于口鼻部、口腔粘膜和/或其中皮肤和蹄壁相交处(冠状带)的开放或闭合性水泡或病变，甲床出血，伴随在冠状带处或周围的发红和肿胀的突然跛行；以及突然停止进食，嗜睡，厌食和/或发烧高达华氏105°(摄氏～40.6°)的繁殖雌性。
看起来存在新生仔猪(小于7天)的死亡率中的短期(4-10天)增加，所述新生仔猪可能具有或没有与之相关的腹泻。关于短时间段的发病率和死亡率估计为30-70％。在调查新生儿死亡率的增加时通常注意到育龄动物中的水泡病变。在猪中导致口鼻部和冠状带水泡的这类感染也已被称为猪中的特发性水泡病。
根据一个进一步方面，本发明还涉及用于预防sva感染的方法，其中所述sva感染可以由塞内卡谷病毒a引起，所述方法包括施用本发明的免疫原性组合物的步骤，所述免疫原性组合物包含如本文提供的一种或多种sva肽。
本发明还提供了制备本文提供的任何免疫原性组合物的方法，该方法包括将如本文提供的一种或多种sva肽与载体分子混合，优选地使得一种或多种sva肽和载体分子是彼此共价偶联或缀合的。此类缀合物可以是多价或单价的。多价组合物或疫苗包括多重sva肽与载体分子的免疫缀合。在一个进一步方面，本发明提供了产生一种或多种sva肽的方法，该方法包括用核酸分子转化宿主细胞，优选原核细胞，例如大肠杆菌(e.coli)，所述核酸分子编码如本文提供的任何sva肽。可替代地，宿主细胞可以是真核细胞，例如动物细胞、原生生物细胞、植物细胞或真菌细胞。优选地，真核细胞是哺乳动物细胞，例如cho、bhk或cos，或真菌细胞，例如酿酒酵母(saccharomycescerevisiae)，或昆虫细胞，例如sf9。
本发明的另一个方面提供了产生一种或多种sva肽的方法，所述一种或多种sva肽诱导针对sva的免疫应答。这包括培养经转化的表达载体，其编码并表达本文公开的一种或多种sva肽。表达的蛋白质或被表达生物保留或被分泌到培养基内。在足以产生能够诱导针对sva的免疫应答的sva肽的条件下进行表达。
还提供了经由表达经修饰的svap1-2a-p3蛋白的重组杆状病毒，产生在昆虫细胞中生成的重组表达的p1-2a-p3抗原疫苗的方法。在一个示例性实施方案中，该方法包括将svap1-2a-p3序列克隆到载体pvl1393(bdbiosciences)内，并且共转染sf9昆虫细胞。对于灭活的重组sva材料，使用5mmbei将sva杆状病毒收获物灭活24小时，澄清且进行0.45μm过滤。通常，灭活的病毒例如通过浓缩以及与其它组分共混进行进一步加工，以产生商业制剂。例如，可以将含有灭活病毒的流体浓缩，并且与佐剂和/或针对一种或多种其它猪病原体的抗原共混。
制备本发明的组合物的方法可以进一步包括将一种或多种sva肽或灭活的全病毒制剂和载体分子的缀合物与生理学可接受的媒介物，例如药学或兽医学可接受的载体、佐剂或其组合混合。本领域技术人员将认识到，媒介物、佐剂或组合的选择尤其由递送途径、个人偏好和动物物种决定。
在另一个方面，本发明提供了诊断对象中的sva感染的方法。该方法包括提供一种或多种sva肽；使一种或多种sva肽与得自对象的样品接触；并且如果在样品中检测到能够结合一种或多种sva肽的抗体，则将对象鉴定为具有sva感染。
在另一个方面，本发明提供了确定对象先前已暴露于sva感染并且能够表达针对sva的免疫应答的方法。该方法包括提供一种或多种sva肽；使一种或多种sva肽与得自对象的样品接触；并且如果在样品中检测到能够结合一种或多种sva肽的抗体，则将对象鉴定为具有sva感染。
本发明还提供了包含以下的试剂盒：免疫原性组合物，所述免疫原性组合物包含一种或多种sva肽，优选地连同载体分子一起；用于包装免疫原性组合物的容器；一组印刷说明书；以及能够将免疫原性组合物施用于动物的分配器。任选地，一种或多种sva肽和载体分子可以包装为缀合物或分开的化合物。当分开供应时，还供应了使一种或多种sva肽和载体分子缀合的工具，以及适当的印刷说明书。
本发明还提供了用于给动物接种疫苗的试剂盒，其包括一组印刷说明书；能够向动物施用包含一种或多种sva肽的本文提供的免疫原性组合物的分配器；并且其中至少一种sva肽针对与sva感染相关的至少一种疾病有效地免疫动物。优选地，一种或多种sva肽选自本文提供的那些。本发明的试剂盒可以进一步包含兽医学可接受的载体、佐剂或其组合。
本发明的试剂盒中的分配器能够将其内容物作为液滴分配；并且当鼻内、经口、皮内或肌内施用于动物时，试剂盒中包括的如本文提供的包含sva肽的免疫原性组合物，能够减少sva感染的至少一种临床体征的严重性。优选地，与未治疗的受感染动物相比，临床体征的严重性减少至少10％，优选至少20％，甚至更优选至少30％，甚至更优选至少50％，甚至更优选至少70％，最优选至少100％。
还公开了用于治疗或预防由sva引起的感染的方法。该方法包括向对象施用有效量的本发明的免疫原性组合物，其中所述治疗或预防选自减少sva感染的体征、减少sva感染的临床体征的严重性或发生率、减少对象因sva感染的死亡率及其组合。
本发明的组合物进一步包含兽医学可接受的载体、佐剂或其组合。此类组合物可以用作疫苗，并且包含灭活疫苗。此类疫苗引起针对与sva相关的至少一种疾病的保护性免疫应答。
本领域技术人员将理解，本文使用的组合物可以掺入已知的可注射的、生理学可接受的无菌溶液。为了制备用于肠胃外注射或输注的即用溶液，等渗水溶液，例如盐水或血浆蛋白溶液是可容易获得的。另外，本发明的免疫原性和疫苗组合物可以包括药学或兽医学可接受的载体、稀释剂、等渗剂、稳定剂或佐剂。
本发明的方法还可以包括将本发明的组合物与兽医学可接受的载体、佐剂或其组合混合。本领域技术人员将认识到，载体、佐剂或组合的选择尤其由递送途径、个人偏好和动物物种决定。
还公开了用于治疗或预防由sva引起的感染的方法。该方法包括向动物施用有效量的本发明的免疫原性组合物，其中所述治疗或预防选自减少sva感染的体征、减少sva感染的临床体征的严重性或发生率、减少动物因sva感染的死亡率及其组合。
优选的施用途径包括鼻内、经口、皮内和肌内。经由肌内途径的施用是优选的，最优选在单一剂量中。本领域技术人员将认识到，本发明的组合物也可以在两个或更多个剂量中，以及通过其它施用途径来施用。例如，此类其它途径包括皮下、皮内、静脉内、血管内、动脉内、腹膜内、鞘内、气管内、皮内、心内、叶内、髓内、肺内或阴道内。取决于治疗的所需持续时间和有效性，本发明的组合物可以一次或几次，以及间歇地施用，例如在每天一次的基础上共几天、几周或几个月，并且以不同的剂量。
本发明还提供了用于给动物接种疫苗的试剂盒，其包括一组印刷说明书；能够向动物施用疫苗的分配器；以及来自sva培养物的至少一种分离株。本发明的试剂盒可以进一步包含兽医学可接受的载体、佐剂或其组合。
本发明的试剂盒中的分配器能够将其内容物作为液滴分配；并且当鼻内、经口、皮内或肌内施用于动物时，试剂盒中包括的分离株能够减少sva感染的至少一种临床体征的严重性。在一些试剂盒中，分离株还能够减少sva感染的至少一种临床体征的严重性。优选地，与未治疗的受感染动物相比，临床体征的严重性减少至少10％。
本发明的其它目的、特征和优点根据下述详细描述将变得显而易见。然而，应该理解，详细描述和具体实例虽然指示了本发明的优选实施方案，但仅通过举例说明的方式给出，因为在本发明的精神和范围内的各种改变和修改根据该详细描述对于本领域技术人员将变得显而易见。
附图说明
图1显示了用于杆状病毒表达系统的svvp13c构建体设计(a)(seqidno：18)，以及svvp13c-co(密码子优化的)构建体设计(b)(seqidno：20)。
图2显示了用于杆状病毒表达系统的svvp1-his-sires-svv3c构建体设计(a)(seqidno：32)，以及svvp1co-his-sires-svv3c(密码子优化的)构建体设计(b)(seqidno：33)。
图3显示了用于杆状病毒表达系统的突变vp3/vp1切割位点的svvp13cvp3/vp1构建体设计(a)(seqidno：22)，以及svvp13cd构建体设计(b)(seqidno：24)。
图4显示了与用抗α-svvvp1、抗α-svvvp2和抗α-svvvp3交叉吸收的兔多克隆抗体检测的天然svv抗原相比，baculofbu/svvp13c(a)(seqidno：18)和baculofbu/svvp13c-co(b)(seqidno：20)上清液样品的蛋白质印迹。泳道a是蛋白质标准，泳道1是svvbaculo收获物上清液，泳道2是svv抗原(seqidno：3)，且泳道3是baculo收获物上清液阴性对照。预期的条带大小：全长svvp1＝约95kda(seqidno：19，氨基酸1-859)，vp1＝约29kda(seqidno：19，氨基酸596-859)，vp2-约32kda(seqidno：19，氨基酸73-356)，vp3＝约26kda(seqidno：19，氨基酸357-595)。
图5显示了用抗α-svvvp1、α-svvvp2和α-svvvp3兔多克隆抗体的天然svv病毒蔗糖级分(a)和baulofbu/svvp13c蔗糖级分(b)的蛋白质印迹。泳道a是蛋白质标准，泳道1-10是蔗糖级分1-10，n-baculofbu/无插入片段阴性对照，p是阳性对照天然灭活的svv，且s是关于蔗糖梯度的起始样品。预期的条带大小：全长svvp1＝约95kda(seqidno：19，氨基酸1-859)，vp1＝约29kda(seqidno：19，氨基酸596-859)，vp2-约32kda(seqidno：19，氨基酸73-356)，vp3＝约26kda(seqidno：19，氨基酸357-595)。
图6显示了用α-svvvp1(顶部行)和α-svvvp3(底部行)兔多克隆抗体的baculofbu/svvp13c(seqidno：18)和baculofbu/vvp13cvp3/vp1(seqidno：22)上清液收获物的蛋白质印迹。泳道a-蛋白质标准，泳道1-svvbaculo收获物上清液，泳道2-svv抗原和泳道3-baculo收获物上清液阴性对照。
图7显示了用抗svvvp1、抗svvvp2和抗svvvp3兔多克隆抗体的baculofbu/vvp13cvp3/vp1(seqidno：22)蔗糖级分的蛋白质印迹。泳道a是蛋白质标准，n是baculofbu/无插入片段天然对照，p是阳性对照天然灭活的svv(seqidno：3)，s是重悬浮于tbs中的svvp13cvp3/vp1(seqidno：22)团块，且泳道1-9是蔗糖级分1-9。全长svvp1＝约95kda(seqidno：19，氨基酸1-859)，vp1＝约29kda(seqidno：19，氨基酸596-859)，vp2-约32kda(seqidno：19，氨基酸73-356)，vp3＝约26kda(seqidno：19，氨基酸357-595)。
图8显示了用抗svvvp1、抗svvvp2和抗svvvp3兔多克隆抗体检测的baculofbu/vvp13cd(seqidno：24)上清液收获物的蛋白质印迹。泳道a是蛋白质标准，泳道1是svvbaculo收获物上清液，泳道2是svv抗原(seqidno：3)，且泳道3是baculo收获物上清液阴性对照。预期的条带大小：全长svvp1＝约95kda(seqidno：19，氨基酸1-859)，vp1＝约29kda(seqidno：19，氨基酸596-859)，vp2-约32kda(seqidno：19，氨基酸73-356)，vp3＝约26kda(seqidno：19，氨基酸357-595)。
图9显示了用抗svvvp1、抗svvvp2和抗svvvp3兔多克隆抗体的balulofbu/svvp13cd(seqidno：24)蔗糖级分的蛋白质印迹。泳道a是蛋白质标准，泳道1是svvbaculo收获物上清液，泳道n是baculofbu/无插入片段阴性对照，p是阳性对照天然灭活的svv(seqidno.3)，s是重悬浮于tba中的svvp13cd(seqidno.25)团块，且泳道1-10是蔗糖级分1-10。预期的条带大小：全长svvp1＝约95kda(seqidno：19，氨基酸1-859)，vp1＝约29kda(seqidno：19，氨基酸596-859)，vp2-约32kda(seqidno：19，氨基酸73-356)，vp3＝约26kda(seqidno：19，氨基酸357-595)。
图10显示了baculofbu/svvp13c(a)(seqidno:18)、baculofbu/svvp13cd(a)(seqidno:24)和baculofbu/svvp13cvp3/vp1(b)(seqidno:22)的蛋白质印迹。第3天可溶性级分具有α-svvvp1、α-svvvp2和α-svvvp3兔多克隆抗体。用于baculofbu/svvp13c和baculofbu/svvp13cd(c)的sds-page凝胶。泳道a-蛋白质标准，泳道p-阳性对照天然svv抗原，泳道1-第3天可溶性级分svvp13c，泳道2-第3天可溶性级分svvp13cd，泳道3-第3天可溶性级分svvp13cvp3/vp1和泳道n-阴性对照baculofbu/无插入片段可溶性级分。预期的条带大小：全长svvp1＝约95kda(seqidno：19，氨基酸1-859)，vp1＝约29kda(seqidno：19，氨基酸596-859)，vp2-约32kda(seqidno：19，氨基酸73-356)，vp3＝约26kda(seqidno：19，氨基酸357-595)。
图11显示了用抗α-svvvp1、抗α-svvvp2和抗α-svvvp3兔多克隆抗体的第3天可溶性baculofbu/svvp13c(seqidno:18)、baculofbu/svvp13cd(seqidno:24)和baculofbu/svvp13cvp3/vp1(seqidno:22)的蔗糖级分的蛋白质印迹。泳道a是蛋白质标准，p是阳性对照天然灭活的svv，泳道1-11是蔗糖级分1-11，且n是阴性对照baculofbu/无插入片段可溶性级分。预期的条带大小：全长svvp1＝约95kda(seqidno：19，氨基酸1-859)，vp1＝约29kda(seqidno：19，氨基酸596-859)，vp2-约32kda(seqidno：19，氨基酸73-356)，vp3＝约26kda(seqidno：19，氨基酸357-595)。
图12显示了按研究天数和组的最小均方直肠温度(℃)。
图13显示了按组和天数，在血清中的组中值log10基因组拷贝/mlsvarna。
发明详述
除非另有说明，否则本发明的实践采用分子生物学、微生物学、重组dna技术、蛋白质化学和免疫学的常规技术，其在本领域的技术内。此类技术在文献中得到充分解释。参见例如，sambrook，fritsch&maniatis，molecularcloning:alaboratorymanual，第i、ii和iii卷，第二版(1989)；dnacloning，第i和ii卷(d.n.glover编辑1985)；oligonucleotidesynthesis(m.j.gait编辑1984)；nucleicacidhybridization(b.d.hames&s.j.higgins编辑1984)；animalcellculture(r.k.freshney编辑1986)；immobilizedcellsandenzymes(irlpress，1986)；perbal，b.，apracticalguidetomolecularcloning(1984)；theseries，methodsinenzymology(s.colowick和n.kaplan编辑，academicpress，inc.)；proteinpurificationmethods–apracticalapproach(e.l.v.harris和s.angal，编辑，irlpressatoxforduniversitypress)；以及handbookofexperimentalimmunology，第i-iv卷(d.m.weir和c.c.blackwell编辑，1986，blackwellscientificpublications)。
在详细描述本发明之前，应理解，本发明并不限于特定的dna、多肽序列或过程参数，因为这些当然可以变化。还应理解，本文使用的术语仅用于描述本发明的特定实施方案的目的，并且不预期是限制性的。必须注意的是，如在本说明书和所附权利要求中使用的，单数形式“一个”、“一种”和“该/所述”包括复数指示物，除非内容另有明确规定。因此，例如，提及“抗原”包括两种或更多种抗原的混合物；提及“赋形剂”包括两种或更多种赋形剂的混合物，等等。
a.定义
除非另有定义，否则本文使用的所有技术和科学术语都具有与本发明在提交时所属领域的技术人员通常理解相同的含义。术语的含义和范围应该是明确的；然而，在任何潜在的歧义的情况下，本文提供的定义优先于任何字典或外部定义。进一步地，除非上下文另有要求，否则单数术语应该包括复数，并且复数术语应该包括单数。在本文中，除非另外说明，否则“或”的使用意指“和/或”。此外，术语“包括(including)”以及其它形式如“包括(includes)”和“包括(included)”的使用不是限制性的。本文提及的所有专利和出版物都引入本文作为参考。
“针对疾病的保护”、“保护性免疫”、“功能性免疫”和类似短语，意指通过施用本发明的一种或多种治疗组合物或其组合，而生成的针对疾病或状况的应答，其导致在已暴露于疾病或感染的非免疫对象中预期的更少的有害效应。即，感染的有害效应的严重性在接种疫苗的对象中减弱。感染在接种疫苗的对象中可以减少，减慢或可能完全预防。在本文中，当意指感染的完全预防时，它被明确陈述。如果未陈述完全预防，则该术语包括部分预防。
在本文中，“临床体征的发生率和/或严重性的减少”或“临床症状的减少”意指，但不限于与野生型感染相比，减少组中的受感染对象数目、减少或消除显示出感染的临床体征的对象数目、或者减少一个或多个对象中存在的任何临床体征的严重性。例如，它应该指病原体载量的任何减少、病原体脱落、病原体传播中的减少、或疟疾的任何临床体征症状的减少。优选地，与未接受该组合物且变得被感染的对象相比，这些临床体征在接受本发明的治疗组合物的一个或多个对象中减少至少10％。更优选地，临床体征在接受本发明的组合物的对象中减少至少20％，优选至少30％，更优选至少40％，且甚至更优选至少50％。
术语“增加的保护”在本文中意指但不限于，相对于未接种疫苗的对象的对照组，在接种疫苗的对象组中，与被传染原优选sva感染相关的一种或多种临床症状的统计学显著减少。术语“临床症状的统计学显著减少”意指但不限于，在传染原攻击后，与未接种疫苗的对照组相比，在接种疫苗的对象组中至少一种临床症状的发生率频率低至少10％，优选20％，更优选30％，甚至更优选50％，且甚至更优选70％。
“持久保护”应该指持续至少3周，但更优选至少3个月，还更优选至少6个月的“改善功效”。在家畜的情况下，最优选持久保护应该持续直到动物在其下因肉而出售的平均年龄。
“免疫原性或免疫学组合物”指包含至少一种sva免疫原性组合物或其免疫原性部分的物质组合物，其在宿主中引发针对该组合物的细胞或抗体介导的免疫应答的免疫学应答。在本发明的一个优选实施方案中，免疫原性组合物诱导免疫应答，并且更优选地，赋予针对sva感染的一种或多种临床体征的保护性免疫。
如本文使用的，“免疫原性”或“抗原”指引发如本文所述的免疫应答的多肽或蛋白质。“免疫原性”sva蛋白或多肽包括本文鉴定的任何sva或其类似物或免疫原性片段的全长序列。术语“免疫原性片段”或“免疫原性部分”指sva的片段或者截短的和/或取代的形式，其包括一个或多个表位并且因此引发本文所述的免疫应答。一般而言，此类截短的和/或取代的形式或片段包含来自全长sva蛋白的至少六个邻接氨基酸。更优选地，截短的或取代的形式或片段具有来自全长sva蛋白的至少10个，更优选至少15个，且还更优选至少19个邻接氨基酸。可以使用本领域众所周知的许多表位作图技术来鉴定此类片段。参见例如，epitopemappingprotocolsinmethodsinmolecularbiology，第66卷(glenne.morris编辑，1996)，humanapress，totowa，newjersey。例如，线性表位可以通过以下进行测定：同时合成在固体支持物上的大量肽，所述肽对应于蛋白质分子的一部分，并且在肽仍附着至载体的同时，使肽与抗体反应。此类技术是本领域已知且描述的，参见例如，美国专利号4,708,871；geysen等人(1984)proc.natl.acad.sci.usa81:3998-4002；以及geysen等人(1986)molec.immunol.23:709-715。类似地，例如通过x射线晶体学和二维核磁共振，通过测定氨基酸的空间构象，容易地鉴定构象表位。参见epitopemappingprotocols，同上。合成抗原也包括在定义内，例如多表位、侧翼表位和其它重组或合成衍生的抗原。参见例如，bergmann等人(1993)eur.j.immunol.23:2777-2781；bergmann等人(1996)，j.immunol.157:3242-3249；suhrbier，a.(1997)，immunol.andcellbiol.75:402-408；以及gardner等人，(1998)12thworldaidsconference，geneva，瑞士，1998年6月28日-7月3日。(其教导和内容全都通过引用引入本文)。
“免疫应答”或“免疫学应答”意指但不限于针对目的组合物或疫苗的细胞和/或抗体介导的免疫应答的发展。通常，免疫应答或免疫学应答包括但不限于下述效应中的一种或多种：特异性针对目的组合物或疫苗中包括的一种或多种抗原的抗体、b细胞、辅助性t细胞、抑制性t细胞和/或细胞毒性t细胞的产生或激活。优选地，宿主展示治疗性或保护性免疫学(记忆)应答，使得对新感染的抗性得以增强和/或疾病的临床严重性得以减少。通过症状数目、症状严重性中的减少、或与病原体感染相关的一种或多种症状的缺乏、病毒血症发作中的延迟、减少的病毒持续性、总体病毒载量中的减少和/或病毒排出的减少来证实此类保护。
在本文中，“特异性免疫反应性”指识别sva感染特有的抗原，但不与严格攻击对照特有的抗原反应的免疫反应性蛋白质或多肽。
如本文使用的，“药学或兽医学可接受的载体”包括任何和所有溶剂、分散介质、包衣、佐剂、稳定试剂、稀释剂、防腐剂、抗菌剂和抗真菌剂、等渗剂、吸附延迟剂等等。在一些优选实施方案中，且尤其是包括冻干的免疫原性组合物的那些实施方案，用于在本发明中使用的稳定试剂包括用于冻干或冷冻干燥的稳定剂。
在一些实施方案中，本发明的免疫原性组合物含有佐剂。如本文使用的，“佐剂”可以包括氢氧化铝和磷酸铝、皂苷例如quila、qs-21(cambridgebiotechinc.，cambridgema)、gpi-0100(galenicapharmaceuticals，inc.，birmingham，al)、油包水型乳液、水包油型乳液、水包油包水型乳液。该乳液可以特别地基于轻质液体石蜡油(欧洲药典型)；类异戊二烯油，例如角鲨烷或角鲨烯；起因于烯烃，特别是异丁烯或癸烯的寡聚化的油；含有线性烷基基团的酸或醇的酯，更特别是植物油、油酸乙酯、丙二醇二(辛酸酯/癸酸酯)、甘油三(辛酸酯/癸酸酯)或丙二醇二油酸酯；支链脂肪酸或醇的酯，特别是异硬脂酸酯。该油与乳化剂组合使用，以形成乳液。乳化剂优选为非离子型表面活性剂，特别是脱水山梨糖醇、二缩甘露醇(例如脱水甘露醇油酸酯)、二醇、聚甘油、丙二醇以及油酸、异硬脂酸、蓖麻油酸或羟基硬脂酸的酯，其任选地被乙氧基化，以及聚氧丙烯-聚氧乙烯共聚物嵌段，特别是pluronic产品，尤其是l121。参见hunter等人，thetheoryandpracticalapplicationofadjuvants(ed.stewart-tull，d.e.s.)，johnwileyandsons，ny，第51-94页(1995)，以及todd等人，vaccine15:564-570(1997)。示例性的佐剂是由m.powell和m.newman，plenumpress，1995编辑的“vaccinedesign，thesubunitandadjuvantapproach”的第147页上描述的spt乳液，以及在这同一本书的第183页上描述的乳液mf59。
佐剂的进一步实例是选自丙烯酸或甲基丙烯酸的聚合物，以及马来酸酐和烯基衍生物的共聚物的化合物。有利的佐剂化合物是丙烯酸或甲基丙烯酸的聚合物，其尤其与糖或多元醇的聚烯基醚交联。这些化合物通过术语卡波姆已知(phameuropa，第8卷，第2期，1996年6月)。本领域技术人员还可以参考美国专利号2,909,462，其描述了与具有至少3个羟基基团，优选不超过8个羟基基团的多羟基化化合物交联的此类丙烯酸聚合物，至少三个羟基的氢原子被替换为具有至少2个碳原子的不饱和脂族原子团。优选的原子团是含有2至4个碳原子的那些原子团，例如乙烯基、烯丙基和其它烯键式不饱和基团。不饱和原子团本身可以含有其它取代基，例如甲基。以名称
进一步的合适佐剂包括但不限于尤其是ribi佐剂系统(ribiinc.)，嵌段共聚物(cytrx，atlantaga)，saf-m(chiron，emeryvilleca)，单磷酰脂质a，阿夫立定脂质-胺佐剂，来自大肠杆菌的热不稳定性肠毒素(重组或其它形式)，霍乱毒素，ims1314或胞壁酰二肽，或者天然存在的或重组的细胞因子或其类似物或内源性细胞因子释放的刺激物。
预期可以以约100μg至约10mg/剂的量，优选以约100μg至约10mg/剂的量，更优选以约500μg至约5mg/剂，甚至更优选以约750μg至约2.5mg/剂的量，且最优选以约1mg/剂的量添加佐剂。可替代地，佐剂可以为按最终产物的体积计约0.01至50％的浓度，优选为约2％至30％的浓度，更优选为约5％至25％的浓度，还更优选为约7％至22％的浓度，且最优选为10％至20％的浓度。
“稀释剂”可以包括水、盐水、右旋糖、乙醇、甘油等等。等渗剂可以尤其包括氯化钠、右旋糖、甘露醇、山梨糖醇和乳糖。稳定剂尤其包括白蛋白和乙二胺四乙酸的碱金属盐。
“分离的”意指“通过人为”从其天然状态改变，即，如果它在自然界中出现，则它已被改变或从其原始环境中取出、或两者。例如，天然存在于活生物中的多核苷酸或多肽不是“分离的”，但是与其天然状态的共存材料分开的相同的多核苷酸或多肽是“分离的”，如该术语在本文中采用的。
“安全性”指在疫苗接种后在接种疫苗的动物中不存在不良后果，包括但不限于：基于细菌的疫苗潜在逆转为毒力，临床上显著的副作用，例如持续的全身性疾病或在疫苗施用的部位处无法接受的炎症。
如本文使用的，术语“疫苗接种(vaccination)”或“疫苗接种(vaccinating)”或其变体意指但不限于，包括施用本发明的免疫原性组合物的过程，当施用于动物时，所述免疫原性组合物在动物中引发或能够引发(直接或间接地)针对sva的免疫应答。
在本发明的上下文中，“死亡率”指由sva感染引起的死亡，并且包括其中感染如此严重使得对动物实施安乐死，以防止痛苦且对其生命提供人道终结的情况。
在本文中，“有效剂量”意指但不限于引发或能够引发免疫应答的抗原量，所述免疫应答在抗原施用于其的动物中产生临床体症状的减少。
如本文使用的，术语“有效量”在组合物的上下文中意指能够诱导免疫应答的免疫原性组合物的量，所述免疫应答在动物中减少感染的发生率或减轻感染的严重性或疾病的发生率。特别地，有效量指集落形成单位(cfu)/剂量。可替代地，在治疗的上下文中，术语“有效量”指这样的疗法量，其足以减少或改善疾病或病症、或者其一种或多种症状的严重性或持续时间，预防疾病或病症的前进，引起疾病或病症的消退，预防与疾病或病症相关的一种或多种症状的复发、发展、发作或进展，或者增强或改善另一种疗法或治疗剂的预防或治疗。
术语“片段”指sva肽或编码此类sva肽的基因的片段或者截短的和/或取代的形式，其包括一个或多个表位并且因此引发针对sva的免疫应答。优选地，此类片段是本文提供的任何sva肽或任何sva基因的片段或者截短的和/或取代的形式。一般而言，此类截短的和/或取代的形式或片段包含来自全长sva序列的至少六个邻接氨基酸。更优选地，截短的或取代的形式或片段具有来自全长sva序列的至少10个，更优选地至少15个，且还更优选地至少19个邻接氨基酸。可以使用本领域众所周知的许多表位作图技术来鉴定此类片段。参见例如，epitopemappingprotocolsinmethodsinmolecularbiology，第66卷(glenne.morris编辑，1996)，humanapress，totowa，newjersey。例如，线性表位可以通过以下进行测定：同时合成在固体支持物上的大量肽，所述肽对应于蛋白质分子的一部分，并且在肽仍附着至载体的同时，使肽与抗体反应。此类技术是本领域已知且描述的，参见例如，美国专利号4,708,871；geysen等人(1984)proc.natl.acad.sci.usa81:3998-4002；以及geysen等人(1986)molec.immunol.23:709-715。类似地，例如通过x射线晶体学和二维核磁共振，通过测定氨基酸的空间构象，容易地鉴定构象表位。参见epitopemappingprotocols，同上。合成抗原也包括在定义内，例如多表位、侧翼表位和其它重组或合成衍生的抗原。参见例如，bergmann等人(1993)eur.j.immunol.23:2777-2781；bergmann等人(1996)，j.immunol.157:3242-3249；suhrbier，a.(1997)，immunol.andcellbiol.75:402-408；以及gardner等人，(1998)12thworldaidsconference，geneva，瑞士，1998年6月28日-7月3日。(其教导和内容全都通过引用引入本文)。
关于序列(例如，多肽或核酸序列)的术语“变体”预期意指基本上相似的序列。对于包含开放读码框的核苷酸序列，变体包括由于遗传密码的简并性，编码天然蛋白质的相同氨基酸序列的那些序列。变体核苷酸序列还包括合成衍生的核苷酸序列，例如通过使用定点诱变生成的那些，并且对于开放读码框，编码天然蛋白质，以及编码相对于天然蛋白质具有氨基酸取代用于密码子优化的目的的多肽的那些。一般地，使用由标准参数描述的比对程序之一，与参考序列相比，本发明的核苷酸序列变体具有至少80％、81％、82％、83％、84％、85％、86％、87％、88％或89％，更优选至少90％、91％、92％、93％或94％，且最优选至少95％、96％、96.1％、96.2％、96.3％、96.4％、96.5％、96.6％、96.7％、96.8％、96.9％、97％、97.1％、97.2％、97.3％、97.4％、97.5％、97.6％、97.7％、97.8％、97.9％、98％、98.1％、98.2％、98.3％、98.4％、98.5％、98.6％、98.7％、98.8％、98.9％、99％、99.1％、99.2％、99.3％、99.4％、99.5％、99.6％、99.7％、99.8％、99.9％的序列相同性。
如本文使用的，术语“对sva具有免疫反应性”意指该肽或片段引发针对sva的免疫学应答。
如本文使用的，“序列同源性”指测定两个序列的相关性的方法。为了测定序列同源性，将两个或更多个序列最佳比对，并且需要时引入缺口。然而，与“序列相同性”形成对比，当测定序列同源性时，保守氨基酸取代被视为匹配。换言之，为了获得与参考序列具有95％序列同源性的多肽或多核苷酸，参考序列中85％，优选90％，甚至更优选95％的氨基酸残基或核苷酸必须匹配或包含由另一种氨基酸或核苷酸的保守取代，或者参考序列中高达15％，优选高达10％，甚至更优选高达5％的总氨基酸残基或核苷酸的多个氨基酸或核苷酸(不包括保守取代)可以插入参考序列中。优选地，同源序列包含至少50个，甚至更优选100个，甚至更优选250个，甚至更优选地500个核苷酸的段。
“保守取代”指氨基酸残基或核苷酸由具有相似特征或性质(包括大小、疏水性等)的另一个氨基酸残基或核苷酸的取代，使得总体功能没有明显改变。
如本领域已知的，“序列相同性”指两个或更多个多肽序列、或者两个或更多个多核苷酸序列之间的关系，即参考序列以及待与参考序列进行比较的给定序列。如通过此类序列的串之间的匹配测定的，在序列已进行最佳比对以产生最高程度的序列相似性后，通过将给定序列与参考序列进行比较来测定序列相同性。在此类比对后，在逐个位置的基础上确定序列相同性，例如，如果在特定位置处核苷酸或氨基酸残基是相同的，则序列在该位置处是“相同的”。然后将此类位置相同性的总数目除以参考序列中核苷酸或残基的总数目，以给出％序列相同性。序列相同性可以通过已知方法容易地计算，所述方法包括但不限于在以下中描述的那些：computationalmolecularbiology，lesk，a.n.，编辑，oxforduniversitypress，newyork(1988)，biocomputing:informaticsandgenomeprojects，smith，d.w.，编辑，academicpress，newyork(1993)；computeranalysisofsequencedata，parti，griffin，a.m.和griffin，h.g.，编辑，humanapress，newjersey(1994)；sequenceanalysisinmolecularbiology，vonheinge，g.，academicpress(1987)；sequenceanalysisprimer，gribskov，m.和devereux，j.，编辑，m.stocktonpress，newyork(1991)；以及carillo，h.和lipman，d.，siamj.appliedmath.，48:1073(1988)，其教导通过引用引入本文。测定序列相同性的优选方法被设计为给出在测试的序列之间的最大匹配。测定序列相同性的方法被编码在测定给定序列之间的序列相同性的可公开获得的计算机程序中。此类程序的实例包括但不限于gcg程序包(devereux，j.等人，nucleicacidsresearch，12(1):387(1984))，blastp、blastn和fasta(altschul，s.f.等人，j.molec.biol.，215:403-410(1990)。blastx程序可从ncbi和其它来源公开获得(blastmanual，altschul，s.等人，ncvinlmnihbethesda，md20894，altschul，s.f.等人，j.molec.biol.，215:403-410(1990)，其教导引入本文作为参考)。这些程序使用缺省的缺口权重来最佳比对序列，以便产生在给定序列和参考序列之间最高水平的序列相同性。作为说明，通过具有与参考核苷酸序列具有至少例如85％，优选90％，甚至更优选95％的“序列相同性”的核苷酸序列的多核苷酸，意指给定多核苷酸的核苷酸序列与参考序列相同，除了给定的多核苷酸序列可以包括高达15个，优选高达10个，甚至更优选高达5个点突变/参考核苷酸序列的每100个核苷酸之外。换言之，在具有相对于参考核苷酸序列具有至少85％，优选90％，甚至更优选95％相同性的核苷酸序列的多核苷酸中，参考序列中高达15％，优选10％，甚至更优选5％的核苷酸可以被缺失或被另一种核苷酸取代，或者参考序列中高达15％，优选10％，甚至更优选5％的总核苷酸的多个核苷酸可以被插入参考序列内。参考序列的这些突变可以在参考核苷酸序列的5'或3'末端位置处、或者在那些末端位置之间的任何位置处出现，或个别地散布在参考序列中的核苷酸中、或散布在参考序列内的一个或多个邻接基团中。类似地，通过具有与参考氨基酸序列具有至少例如85％，优选90％，甚至更优选95％的序列相同性的给定氨基酸序列的多肽，意指多肽的给定氨基酸序列与参考序列相同，除了给定的多肽序列可以包括高达15个，优选高达10个，甚至更优选高达5个氨基酸改变/参考氨基酸序列的每100个氨基酸之外。换言之，为了获得与参考氨基酸序列具有至少85％，优选90％，甚至更优选95％的序列相同性的给定多肽序列，参考序列中高达15％，优选高达10％，甚至更优选高达5％的氨基酸残基可以被缺失或被另一种氨基酸取代，或者参考序列中高达15％，优选高达10％，甚至更优选高达5％的氨基酸残基总数目的多个氨基酸可以被插入参考序列内。参考序列的这些改变可以在参考氨基酸序列的氨基或羧基末端位置处、或者在那些末端位置之间的任何位置处出现，或个别地散布在参考序列中的残基中、或散布在参考序列内的一个或多个邻接基团中。优选地，并不相同的残基位置通过保守氨基酸取代而不同。然而，当测定序列相同性时，保守取代不包括作为匹配。
术语“序列相同性”或“百分比相同性”在本文可互换使用。为了本发明的目的，在本文中定义，为了测定两个氨基酸序列或两个核酸序列的相同性百分比，序列为了最佳比较的目的进行比对(例如，可以在第一氨基酸或核酸序列中引入缺口，用于与第二氨基酸或核酸序列的最佳比对)。然后比较在相应的氨基酸或核苷酸位置处的氨基酸或核苷酸残基。当第一序列中的位置由与第二序列中的相应位置相同的氨基酸或核苷酸残基占据时，则分子在该位置处是相同的。两个序列之间的相同性百分比是由序列共享的相同位置数目的函数(即，％相同性＝相同位置数目/位置(即重叠位置)总数目x100)。优选地，两个序列是相同长度的。
可以在待比较的两个序列的整个长度上或在两个序列的片段上进行序列比较。且在本发明的范围内通常且优选的，比较将在待比较的两个序列的全长上进行。然而，序列相同性可以在例如二十、五十、一百个或更多个邻接基酸残基的区域上进行。
如本文使用的，特别应理解，术语“与根据seqidno：y的核酸/氨基酸序列具有至少x％的序列相同性”(或可替代地，术语“与seqidno：y的/中所示的核酸/氨基酸序列具有至少x％的序列相同性”)分别等价于术语“在seqidno：y的长度上，与根据seqidno：y的核酸/氨基酸序列具有至少x％的序列相同性”、或术语“在seqidno：y的整个长度上，与根据seqidno：y的核酸/氨基酸序列具有至少x％的序列相同性”。
用于表达的载体以及用于制备和/或使用载体(或重组体)的方法可以通过以下中公开的方法或类似于以下中公开的方法：关于dna病毒载体，尤其是美国专利号4,603,112、4,769,330、5,174,993、5,505,941、5,338,683、5,494,807、4,722,848、5,942,235、5,364,773、5,762,938、5,770,212、5,942,235、382,425，pct公开wo94/16716、wo96/39491、wo95/30018；paoletti，"applicationsofpoxvirusvectorstovaccination:anupdate，"pnasusa93:11349-11353，1996年10月；moss，"geneticallyengineeredpoxvirusesforrecombinantgeneexpression，vaccination，andsafety,"pnasusa93:11341-11348，1996年10月；smith等人，美国专利号4,745,051(重组杆状病毒)；richardson，c.d.(编辑)，methodsinmolecularbiology39，"baculovirusexpressionprotocols"(1995humanapressinc.)；smith等人，"productionofhumanbetainterferonininsectcellsinfectedwithabaculovirusexpressionvector"，molecularandcellularbiology，1983年12月，第3卷，第12期，第2156-2165页；pennock等人，"strongandregulatedexpressionofescherichiacolib-galactosidaseininfectcellswithabaculovirusvector，"molecularandcellularbiology，1984年3月，第4卷，第3期，第406页；epa0370573；于1986年10月16日提交的美国申请号920,197；ep专利公开号265785；美国专利号4,769,331(重组疱疹病毒)；roizman，"thefunctionofherpessimplexvirusgenes:aprimerforgeneticengineeringofnovelvectors,"pnasusa93:11307-11312，1996年10月；andreansky等人，"theapplicationofgeneticallyengineeredherpessimplexvirusestothetreatmentofexperimentalbraintumors,"pnasusa93:11313-11318，1996年10月；robertson等人，"epstein-barrvirusvectorsforgenedeliverytoblymphocytes"，pnasusa93:11334-11340，1996年10月；frolov等人，"alphavirus-basedexpressionvectors:strategiesandapplications,"pnasusa93:11371-11377，1996年10月；kitson等人，j.virol.65，3068-3075，1991；美国专利号5,591,439、5,552,143；wo98/00166；两者均于1996年7月3日提交的批准的美国申请序列号08/675,556和08/675,566(重组腺病毒)；grunhaus等人，1992，"adenovirusascloningvectors,"seminarsinvirology(第3卷)第237-52页，1993；ballay等人embojournal，第4卷，第3861-65页，graham，tibtech8，85-87，1990年4月；prevec等人，j.genvirol.70，42434；pctwo91/11525；felgner等人(1994)，j.biol.chem.269，2550-2561，science，259:1745-49，1993；以及mcclements等人，"immunizationwithdnavaccinesencodingglycoproteindorglycoproteinb，aloneorincombination，inducesprotectiveimmunityinanimalmodelsofherpessimplexvirus-2disease"，pnasusa93:11414-11420，1996年10月；以及美国专利号5,591,639、5,589,466和5,580,859，以及wo90/11092、wo93/19183、wo94/21797、wo95/11307、wo95/20660；tang等人，nature，以及furth等人，analyticalbiochemistry。还参见wo98/33510；ju等人，diabetologia，41:736-739，1998(慢病毒表达系统)；sanford等人，美国专利号4,945,050；fischbach等人(intracel)；wo90/01543；robinson等人，seminarsinimmunology第9卷，第271-283页(1997),(dna载体系统)；szoka等人，美国专利号4,394,448(将dna插入活细胞内的方法)；mccormick等人，美国专利号5,677,178(致细胞病变病毒的使用)；以及美国专利号5,928,913(用于基因递送的载体)；以及本文引用的其它文件。
优选的病毒载体包括杆状病毒，例如baculogold(bdbiosciencespharmingen，sandiego，calif.)，特别条件是生产细胞是昆虫细胞。尽管杆状病毒表达系统是优选的，但本领域技术人员应理解，其它表达系统也可用于本发明的目的。
b.载体分子
本发明的sva肽可以与之缀合或共价连接的载体分子优选是上文描述的那些。用于动物使用的优选载体是牛血清白蛋白和钥孔血蓝蛋白。适合于人使用的蛋白质载体包括破伤风类毒素、白喉类毒素、无细胞百日咳疫苗(lpf类毒素)、交叉反应材料(crm)，其在抗原上与细菌毒素相似，但借助于突变而是无毒的。例如，可以使用根据pappenheimer等人，immunochemistry，9，891-906(1972)获得的crm197，以及其它细菌蛋白载体，例如脑膜炎球菌外膜蛋白。优选地，载体蛋白本身是免疫原。
本发明的sva肽可以通过本领域已知的任何方便的方法共价偶联至载体。虽然如通过schneerson等人，j.experimentalmedicine，152，361-376(1980)描述的对称接头例如己二酸二酰肼的使用，或如通过fattom等人，infectionandimmunity，56，2292-2298(1988)描述的异双功能接头例如n-琥珀酰亚胺基3-(2-吡啶基二硫基)丙酸酯的使用在本发明的范围内，优选避免使用任何接头，而是将本发明的sva肽直接偶联至载体分子。如通过landi等人j.immunology，127，1011-1019(1981)所述，可以借助于还原胺化来实现此类偶联。
如通过平均分子量定义的，免疫原性组合物的大小是可变的，并且取决于选择的sva肽和sva肽与载体的偶联方法。因此，它可以小至1,000道尔顿(103)或大于106道尔顿。用还原胺化偶联方法，sva肽的分子量通常在5,000至500,000，例如300,000至500,000，或例如5,000至50,000道尔顿的范围内。
可以通过本领域已知的各种方法来产生特异性结合本发明的sva肽的载体分子，即肽、其衍生物和类似物以及肽模拟物，所述方法包括但不限于固相合成或通过溶液(nakanishi等人，1993，gene137:51-56；merrifield，1963，j.am.chem.soc.15:2149-2154；neurath，h.等人，编辑，theproteins，第ii卷，第3版，第105-237页，academicpress，newyork，n.y.(1976)，其整体通过引用引入本文)。
本发明的sva肽或者本发明的抗体或其结合部分可以通过在具有药学或兽医学载体的稀释剂中的溶液或悬浮液，以可注射剂量施用。
此类分子的毒性和治疗功效可以通过细胞培养或实验动物中，例如用于测定ld50(对50％的群体致死的剂量)的标准药学程序来测定。
本发明的疫苗可以是多价的或单价的。多价疫苗由多重sva肽与载体分子的免疫缀合制成。
在一个方面，sva肽组合物包含有效免疫量的免疫原性缀合物，优选与免疫刺激剂组合；以及生理学可接受的载体。如本上下文中使用的，“免疫刺激剂”预期涵盖具有增强免疫系统活性的能力的任何化合物或组合物，无论它是与特定抗原组合的特异性增强效应，还是简单地对免疫应答的一种或多种元件的活性的独立效应。免疫刺激剂化合物包括但不限于矿物质凝胶，例如氢氧化铝；表面活性物质，如溶血卵磷脂，
需要时，组合物可以存在于包装或分配器装置中，其可以包含含有活性成分的一种或多种单位剂型。包装可以例如包含金属或塑料箔，如泡罩包装。包装或分配器装置可以伴随有施用说明书，优选用于施用于哺乳动物，尤其是猪。与此类容器相关的可以是以由管理药物或生物制品制造、使用或销售的政府机构规定形式的通知，所述通知反映了由制造、使用或销售机构批准用于人施用。
c.佐剂
为了进一步增加本文提供的并且含有一种或多种sva肽的免疫原性组合物的免疫原性，还可以包含一种或多种佐剂。
佐剂可以通过先前描述的或本领域已知的任何技术来纯化。优选的纯化技术是硅胶色谱，特别是“闪速”(快速)色谱技术，如通过w.clarkstill等人，j.organicchemistry，43，2923-2925(1978)所述。然而，其它色谱方法，包括hplc，也可以用于佐剂的纯化。结晶也可以用于纯化佐剂。在一些情况下，不需要纯化，因为直接从合成获得分析纯度的产物。
通过根据已知技术在适当的无菌条件下，将佐剂与sva肽物理混合以产生佐剂化组合物，来制备本发明的疫苗组合物。sva肽与佐剂的络合通过在缀合物上净负电荷的存在得到促进，所述净负电荷被静电吸引至长链烷基化合物佐剂上存在的正电荷。
预期可以以约100μg至约10mg/剂的量，优选以约100μg至约10mg/剂的量，更优选以约500μg至约5mg/剂，甚至更优选以约750μg至约2.5mg/剂的量，且最优选以约1mg/剂的量添加佐剂。可替代地，佐剂可以为按最终产物的体积计约0.01％至75％的浓度，优选为约2％至30％的浓度，更优选为约5％至25％的浓度，还更优选为约7％至22％的浓度，且最优选为10％至20％的浓度。
d.生理学可接受的媒介物
本发明的疫苗组合物可以使用类似于用于其它药物多肽组合物的技术进行配制。因此，佐剂以及优选与载体分子缀合和/或与佐剂混合的sva肽可以以冻干形式贮存，并且在施用前在生理学可接受的媒介物中重构以形成悬浮液。可替代地，佐剂和缀合物可以贮存于媒介物中。优选的媒介物是无菌溶液，特别是无菌缓冲溶液，例如磷酸盐缓冲盐水。将佐剂和缀合物在媒介物中组合使得改善免疫原性组合物的免疫学有效性的任何方法都是适当的。
本发明的疫苗的单一剂量的体积可以变化，但一般在常规疫苗中通常采用的范围内。在上述缀合物和佐剂的浓度下，单一剂量的体积优选在约0.1ml至约3ml之间，优选在约0.2ml至约1.5ml之间，更优选在约0.2ml至约0.5ml之间。
本发明的疫苗组合物可以通过任何方便的手段施用。
e.制剂
包含与载体分子偶联的sva肽的免疫原性缀合物可以用作用于针对sva免疫的疫苗。包含在生理学可接受的媒介物中的免疫原性缀合物的疫苗可用于免疫动物，优选猪的方法中，用于治疗或预防通过sva的感染。
通过用免疫原性缀合物免疫而生成的针对本发明的免疫原性缀合物的抗体可以用于被动免疫疗法和生成用于治疗或预防sva感染的抗独特型抗体。
组合物施用于其的对象优选是动物，包括但不限于牛、马、绵羊、猪、家禽(例如鸡)、山羊、猫、犬、仓鼠、小鼠和大鼠，最优选地哺乳动物是猪。
本发明的制剂包含有效免疫量的一种或多种免疫原性组合物或针对其的抗体和生理学可接受的媒介物。疫苗包含有效免疫量的一种或多种免疫原性组合物和生理学可接受的媒介物。该制剂应该适合施用模式。
需要时，免疫原性组合物还可以含有少量的润湿剂或乳化剂或ph缓冲剂。免疫原性组合物可以是液体溶液、悬浮液、乳剂、片剂、丸剂、胶囊、缓释制剂或粉末。经口制剂可以包括标准载体，例如药物级别的甘露醇、乳糖、淀粉、硬脂酸镁、糖精钠、纤维素、碳酸镁等。
f.有效剂量
本文所述的化合物可以以治疗有效剂量施用于对象，以治疗sva相关的疾病。剂量将取决于接受疫苗的宿主，以及诸如宿主的大小、重量和年龄等因素。
待在制剂中采用的本发明的免疫原性缀合物或抗体的精确量取决于施用途径和对象的性质(例如，物种、年龄、大小、疾病的阶段/水平)，并且应该根据从业者的判断和每个对象的情况根据标准临床技术来决定。有效免疫量是足以治疗或预防对象中的sva传染病的量。适当剂量的实例是约6至7logtcid50/ml。可替代地，有效剂量也可以从衍生自动物模型测试系统的剂量应答曲线外推，并且可以从0.001mg/kg到100mg/kg不等。
化合物的毒性和治疗功效可以通过细胞培养或实验动物中，例如用于测定ld50(对50％的群体致死的剂量)和ed50(在50％的群体中治疗有效的剂量)的标准药学程序来测定。毒性和疗效之间的剂量比是治疗指数，并且它可以表示为比率ld50/ed50。显示出大治疗指数的化合物是优选的。虽然可以使用显示出毒性副作用的化合物，但应该小心设计将此类化合物靶向受影响组织的部位的递送系统，以便最小化对未感染细胞的潜在损害，且从而减少副作用。
从细胞培养测定和动物研究中获得的数据可以用于制定用于在动物，尤其是猪中使用的剂量范围。此类化合物的剂量优选位于循环浓度的范围内，所述循环浓度包括具有很少毒性或没有毒性的ed50。剂量可以在这个范围内变化，取决于所采用的剂型和所利用的施用途径。对于本发明的方法中使用的任何化合物，治疗有效剂量可以最初由细胞培养测定估计。剂量可以在动物模型中制定，以达到包括如在细胞培养中测定的ic50(即，达到症状的半最大抑制的测试化合物浓度)的循环血浆浓度范围。此类信息可以用于更准确地测定在对象中的有用剂量。血浆中的水平可以例如通过高效液相色谱进行测量。
组合物的免疫原性可以通过使用本领域已知的任何免疫测定，监测在用组合物免疫后的测试对象的免疫应答来测定。体液(抗体)应答和/或细胞介导的免疫的生成可以视为免疫应答的指示。测试对象可以包括动物，例如猪、小鼠、仓鼠、犬、猫、兔、牛、马、羊、家禽(例如鸡、鸭、鹅和火鸡)。
可以通过各种方法来分析测试对象的免疫应答，所述方法例如：如通过已知技术例如酶联免疫吸附测定(elisa)、免疫印迹、免疫沉淀等测定的，所得的免疫血清对免疫原性缀合物的反应性；或者通过保护免疫宿主免于通过病原体的感染和/或减弱免疫宿主中由于通过病原体感染的症状，如通过本领域已知用于测定传染病原的水平，例如细菌水平(例如，通过培养来自对象的样品)的任何方法，或本领域已知的其它技术测定的。传染病原的水平也可以通过测量免疫球蛋白针对其的抗原的水平来测定。传染病原的水平中的降低或传染病症状的改善指示该组合物是有效的。
在体内用于动物或人之前，本发明的治疗剂可以在体外测试所需治疗或预防活性。例如，可以用于测定是否指示特定治疗剂的施用的体外测定包括体外细胞培养测定，其中来自细胞系的适当细胞或者从患有特定疾病或病症的对象培养的细胞暴露于或以其它方式施用治疗剂，并且观察到治疗剂对细胞的作用。
可替代地，治疗剂可以通过以下进行测定：使治疗剂与易受传染病原感染但未被传染病原感染的细胞(从对象培养或培养的细胞系培养)接触，使细胞暴露于传染病原，然后测定与治疗剂接触的细胞的感染率是否低于未与治疗剂接触的细胞的感染率。可以通过本领域已知的任何方法来测定细胞被传染病原的感染。
另外，可以通过在治疗之前、过程中或之后的合适时间间隔，测量动物模型或人对象中抗体针对其的分子水平来评价治疗剂。可以鉴定分子的量中的任何变化或变化的不存在，并且与治疗对对象的作用相关联。分子的水平可以通过本领域已知的任何方法测定。
使用本发明的方法和组合物使动物对sva感染疫苗接种后，本领域已知的任何结合测定可以用于评价所得到的抗体与特定分子之间的结合。也可以进行这些测定，以选择对于特定抗原显示出更高亲和力或特异性的抗体。
g.检测和诊断方法
起因于本发明的sva肽的使用的抗体或其结合部分可用于检测样品中sva病毒的存在。这种检测方法包括以下步骤：提供针对本发明的sva肽的分离的抗体或其结合部分，向分离的抗体或其结合部分中加入怀疑含有一定数量的sva的样品，并且检测包含与sva结合的分离的抗体或其结合部分的复合物的存在。
本发明的抗体或其结合部分也可用于检测样品中sva肽的存在。这种检测方法包括以下步骤：提供针对sva肽的分离的抗体或其结合部分，向分离的抗体或其结合部分中加入怀疑含有一定数量的sva肽的样品，并且检测包含与sva肽结合的分离的抗体或其结合部分的复合物的存在。
如本文所述，结合其为结合对成员的特定分子的免疫球蛋白，特别是抗体(及其功能活性片段)可以用作诊断剂和预后剂。在各种实施方案中，本发明提供了结合对成员的测量，以及此类测量在临床应用中的用途。本发明中的免疫球蛋白可以用于例如检测生物样品中的抗原，由此可以测试对象的免疫球蛋白与之结合的分子的异常水平、和/或此类分子的异常形式的存在。“异常水平”意指相对于来自身体的一部分或没有疾病的对象的类似样品中存在的水平、或者代表其的标准水平的增加或降低。本发明的抗体也可以作为试剂包括在用于诊断或预后技术的试剂盒中。
在一个方面，与sva肽免疫特异性结合的本发明的抗体可以用于诊断、预后或筛查sva感染。
在另一个方面，本发明提供了诊断或筛选sva感染或针对其的免疫的存在的方法，其包括在其中所述抗体免疫特异性结合sva肽的对象中，测量抗体与衍生自对象的样品的免疫特异性结合的水平，其中相对于来自没有传染病原的对象的类似样品中的所述免疫特异性结合的水平，所述免疫特异性结合的水平中的增加指示sva的存在。
检测sva肽或其拮抗剂的存在的合适测定的实例包括但不限于elisa、放射免疫测定、凝胶扩散沉淀反应测定、免疫扩散测定、凝集测定、荧光免疫测定、蛋白a免疫测定或免疫电泳测定。
用于特定分子的免疫测定通常包括在可检测地标记的抗体的存在下，温育样品，例如生物流体、组织提取物、新鲜收获的细胞或培养的细胞的裂解产物，并且通过本领域众所周知的许多技术中的任一种检测结合的抗体。
可以根据众所周知的方法测定给定抗体的结合活性。本领域技术人员能够通过采用常规实验来测定用于每次测定的操作和最佳测定条件。
本发明的另一个方面涉及用于检测或测量sva的诊断试剂盒。提供了用于诊断用途的试剂盒，其包含在一个或多个容器中的抗sva肽抗体，以及任选地与该抗体的标记的结合配偶体。可替代地，抗sva肽抗体可以是被标记的(用可检测的标记物，例如化学发光、酶促、荧光或放射性部分)。相应地，本发明提供了诊断试剂盒，其包含抗sva肽抗体和对照免疫球蛋白。在一个具体实施方案中，容器的前述化合物之一可以进行可检测地标记。试剂盒可以任选地进一步包含在容器中的预定量的由试剂盒的抗体识别的sva肽，用于用作标准或对照。
优选的施用途径包括但不限于鼻内、经口、皮内和肌内。经由肌内途径，最优选在单一剂量中的施用是期望的。本领域技术人员将认识到，本发明的组合物也可以在两个或更多个剂量中，以及通过其它施用途径来施用。例如，此类其它途径包括皮下、皮内、静脉内、血管内、动脉内、腹膜内、鞘内、气管内、皮内、心内、叶内、髓内、肺内和阴道内。取决于治疗的所需持续时间和有效性，本发明的组合物可以一次或几次，以及间歇地施用，例如在每天一次的基础上共几天、几周或几个月，并且以不同的剂量。
包括下述实施例以证实本发明的优选实施方案。本领域技术人员应该了解，下述实施例中公开的技术代表由发明人发现的在本发明的实践中发挥良好作用的技术，且因此可以视为构成用于其实践的优选模式。然而，根据本公开内容，本领域技术人员应了解，可以在所公开的特定实施例中进行许多改变，并且仍获得同样或相似的结果，而不脱离本发明的精神和范围。
序列表
本申请含有序列表。序列表包括下述序列：
seqidno：1表示本发明的sva毒株的接近完整的基因组的rna序列。
seqidno：2表示编码病毒多蛋白(svv抗原；seqidno：3)的基因组rna序列的子集。
seqidno：3表示由seqidno：2编码的病毒多蛋白(svv抗原)的氨基酸序列。
seqidno：4表示genbank_kx377924。
seqidno：5表示genbank_kt321458。
seqidno：6表示genbank_kr063107。
seqidno：7表示genbank_kr063108。
seqidno：8表示genbank_kr063109。
seqidno：9表示genbank_hj999048。
seqidno：10表示genbank_kc667560。
seqidno：11表示genbank_gy488390。
seqidno：12表示genbank_gv614995。
seqidno：13表示genbank_dm060849。
seqidno：14表示genbank_nc_011349。
seqidno：15表示genbank_gy488390_cds。
seqidno：16表示genbank_gv614995_cds。
seqidno：17表示genbank_dm060849_cds。
seqidno：18表示构建体baculofbu/svvp13c(a)中的svvp13c插入的核苷酸序列。
seqidno：19表示从baculofbu/svvp13c表达的svvp13c多蛋白的氨基酸序列。
seqidno：20表示构建体baculofbu/svvp13c-co中的svvp13c插入的核苷酸序列，其中svvp1区进行密码子优化用于在昆虫细胞中表达(b)。
seqidno：21表示从baculofbu/svvp13c-co表达的svvp13c多蛋白的氨基酸序列。
seqidno：22表示baculofbu/vvp13cvp3/vp1。
seqidno：23表示构建体baculofbu/svvp13cvp3/vp1的svvp13cvp3/vp1插入的核酸序列。
seqidno：24表示构建体baculofbu/svvp13cd的svvp13cd插入的核酸序列。
seqidno：25表示从baculofbu/svvp13cd表达的svvp13cd多蛋白的氨基酸序列。
seqidno：26表示含有关于svvp1-his的编码序列的pcr产物的核酸序列。
seqidno：27表示从构建体baculog/svvp1-his-sires-svv3c表达的svvp1-his的氨基酸序列。
seqidno：28表示含有关于svvp1-his的编码序列的pcr产物的核酸序列，所述编码序列已进行密码子优化用于在昆虫细胞中表达(svvp1co-his)。
seqidno：29表示从构建体baculog/svvp1co-his-sires-svv3c表达的svvp1-his的氨基酸序列。
seqidno：30表示含有关于svv3c的编码序列的pcr产物的核酸序列。
seqidno：31表示从构建体baculog/svvp1-his-sires-svv3c和baculog/svvp1co-his-sires-svv3c表达的svv3c的氨基酸序列。
seqidno：32表示porb-svvp1-his-sires-svv3c中的svvp1-his-sires-svv3c表达盒的核酸序列。
seqidno：33表示porb-svvp1co-his-sires-svv3c中的svvp1co-his-sires-svv3c表达盒的核酸序列。
seqidno：34表示pcr引物p3219012a(svvp1正向)。
seqidno：35表示pcr引物p3219039a(svvp1his反向)。
seqidno：36表示pcr引物p3219012c(svvp1-co正向)。
seqidno：37表示pcr引物p3219039b(svvp1-cohis反向)。
seqidno：38表示pcr引物p3219012e(svv3c正向)。
seqidno：39表示pcr引物p3219039c(svv3c反向)。
seqidno：40表示pcr引物p3219165a(vp3/vp1正向)。
seqidno：41表示pcr引物p3219165b(vp3/vp1反向)。
seqidno：42表示pcr引物p3219166a(svv3d正向)。
seqidno：43表示pcr引物p3219166b(svv3c反向)。
seqidno：44表示pcr引物p3219166c(svv3d反向)。
seqidno：45表示svv3d编码序列。
本发明进一步包括下述条款：
1.一种在哺乳动物中生成免疫应答的方法，其包括施用免疫学有效量的杀死的塞内卡谷病毒a(sva)，其包含：
(a)seqidno：1或seqidno：2的核酸序列，或
(b)与seqidno：197％相同的核酸序列，其编码具有seqidno：3的多肽的免疫学有效活性的多肽。
2.一种在哺乳动物中生成免疫应答的方法，其包括施用免疫学有效量的杀死的sva，其包含：
(a)具有seqidno：3的氨基酸序列；
(b)具有与seqidno：380％相同的氨基酸序列，并且具有由seqidno：3编码的多肽的生物学或免疫学有效活性；或
(c)其为seqidno：3的氨基酸序列的片段，其包含seqidno：3的至少15个邻接氨基酸并且具有免疫学有效活性。
3.一种在哺乳动物中生成免疫应答的方法，其包括施用免疫学有效量的杀死的sva，其包含：
(a)seqidno：1或seqidno：2的核酸序列，或
(b)与seqidno：1或seqidno：297％相同的核酸序列，其编码具有seqidno：3的多肽的免疫学有效活性的多肽。
4.一种在哺乳动物中生成免疫应答的方法，其包括施用免疫有效量的根据条款1的免疫原性组合物。
5.一种在哺乳动物中生成免疫应答的方法，其包括施用免疫有效量的根据条款2的免疫原性组合物。
6.一种在哺乳动物中生成免疫应答的方法，其包括施用免疫有效量的根据条款3的免疫原性组合物。
7.根据条款1的方法，其中所述哺乳动物是猪，并且所述免疫应答提供对由sva感染引起的疾病的保护性免疫。
8.根据条款2的方法，其中所述哺乳动物是猪，并且所述免疫应答提供对由sva感染引起的疾病的保护性免疫。
9.根据条款3的方法，其中所述哺乳动物是猪，并且所述免疫应答提供对由sva感染引起的疾病的保护性免疫。
10.一种疫苗，其包含塞内卡病毒a(sva)的一种或多种抗原，其中所述sva是包含以下的任何sva：
(a)由seqidno：1编码，和/或包含seqidno：1的序列和/或包含seqidno：1的rna等价物的核酸；
(b)其序列与seqidno：1至少99％相同和/或与seqidno：1的rna等价物至少99％相同的核酸；
(c)由包含seqidno：1或2的序列的多核苷酸编码的多肽；
(d)由与a)的多核苷酸至少80％同源或相同的多核苷酸编码的多肽；
(e)由多核苷酸编码的蛋白质片段，所述多核苷酸包含seqidno：1或seqidno：2的序列中包括的至少15个，优选24个，更优选30个，甚至更优选45个邻接核苷酸；
(f)包含seqidno：3的氨基酸序列的多肽；
(g)与f)的多肽至少80％同源和/或相同的多肽；
(h)f)和/或g)的多肽的片段；
(i)f)或g)的多肽；或
(j)h)或i)的片段，其包含seqidno：3的序列中包括的至少5个，优选8个，更优选10个，甚至更优选15个邻接氨基酸。
11.条款10的疫苗，其中所述疫苗是重组疫苗或杀死的疫苗。
12.条款10的疫苗，其中所述疫苗是杀死的疫苗。
13.条款10的疫苗，其中所述塞内卡病毒a(sva)是化学灭活的。
14.条款13的疫苗，其中所述塞内卡病毒a(sva)通过用化学灭活剂处理进行化学灭活，所述化学灭活剂包括选自乙烯亚胺、二元乙烯亚胺、乙酰乙烯亚胺及其混合物的化合物。
15.条款14的疫苗，其中所述塞内卡病毒a(sva)通过用二元乙烯亚胺处理进行化学灭活。
16.条款10的疫苗，其中所述疫苗进一步包含佐剂。
17.条款16的疫苗，其中所述佐剂是
18.条款6的疫苗，其中所述塞内卡病毒a(sva)包含seqidno：1，和/或包含seqidno：1的rna等价物。
19.条款11的疫苗，其中所述疫苗是重组疫苗。
20.条款11的疫苗，其中此类重组疫苗包含选自以下的一种或多种免疫原性组分：
(a)编码塞内卡病毒a(sva)抗原的分离的核酸，其中所述重组多肽与seqidno：3具有至少90％的同源性，
(b)包含a)的分离的核酸的载体，
(c)由a)的核酸编码的重组蛋白，和
(d)其任何组合。
21.条款20的疫苗，其中此类疫苗包含药学可接受的载体和/或赋形剂。
22.条款21的疫苗，其中所述赋形剂是一种或多种佐剂。
23.条款22的疫苗，其中所述佐剂是
24.条款20的疫苗，其中所述疫苗进一步包含一种或多种另外的抗原。
25.条款20的疫苗，其中免疫原性组分是分离的核酸。
26.条款20的疫苗，其中免疫原性组分是载体。
27.条款11的疫苗，其中免疫原性组分是重组塞内卡病毒a(sva)p1蛋白。
28.条款20的疫苗，其中免疫原性组分是组合。
实施例
实施例1
这项研究利用常规动物来测定疫苗施用后的血清学应答诱导的初步可行性。这项研究的主要目的是评估使用塞内卡病毒a(sva)(seqidno：1或seqidno：2，和/或与seqidno：197％相同的核酸序列，其编码具有seqidno：3的多肽的免疫学有效活性的多肽)的灭活的全病毒制剂，在常规猪中是否血清转化为sva疫苗。
对于病毒分离，通过0.2/0.8μm注射器式滤器(pallacrodisc目录4658)过滤0.5ml水泡液，并且将滤液用于接种到猪睾丸细胞(st细胞)上。st细胞在6孔板中生长至80-100％融合。抽吸培养基，并且将0.25ml滤液接种到细胞上。在37℃下的一小时吸附后，将简单的无血清培养基加入细胞中。使板在37℃、5％co2大气下温育，并且每天检查致细胞病变效应(cpe)。cpe通常在24-28小时内完成。收获的病毒传代几次且用于生成系列。
通过用5ml病毒原液和160ml培养基(最低基础培养基+2.5％hepes)接种用ai-st细胞种植的烧瓶来制备病毒原液。使烧瓶温育大约48小时。将烧瓶冷冻，然后在室温下解冻。材料进行0.2μm过滤。用10mmbei和0.2％甲醛的组合来灭活病毒，伴随在37℃下72小时的恒定搅动。用硫代硫酸钠(添加了17％的bei体积)和亚硫酸氢钠中和灭活。灭活通过材料在ai-st细胞上的两次传代加以确认。对于最初的传代，将10ml灭活的材料接种到ai-st细胞的t75烧瓶上。使烧瓶在37℃+5％co2下温育7天，并且定期评估致细胞病变效应的存在。对于第二次传代，将烧瓶冷冻，然后解冻。将材料离心并且将10ml上清液接种到ai-st细胞的t75烧瓶上。使烧瓶在37℃+5％co2下温育7天，并且定期评估致细胞病变效应的存在。测定中包括阳性和阴性对照样品。结果确认这两个批次中的生长缺乏。使用10k，650cm2超滤中空纤维药液筒，将灭活的病毒收获物浓缩至12.4x。将浓缩的系列与
如表1中所示，将二十头猪随机分成两组。关于组的描述和住房结构，参见下表1。在d0时，向猪施用2ml肌内剂量的sva疫苗或安慰剂。在第21时，动物接受sva疫苗或安慰剂的加强施用。在每次疫苗接种(d0和d21)和d35时的治疗施用之前，从所有猪中收集血液。就血清转化为sva的证据测定血清样品的子集。在研究自始至终记录一般健康观察。在疫苗施用后观察注射部位的反应最少三天。在试验结束时对动物实施人道安乐死。
表1：研究设计
这项研究证实，在施用两个剂量的用12.5％
表2.关键事件和样品收集的时间表
*注意观察持续直到反应消退
为了避免偏差，通过不涉及动物临床监测的人员在d0和d21时施用治疗。在d0时，使用适当大小的无菌针头和注射器，将2ml剂量的疫苗施用于健康猪的右颈部的肌肉组织内。在d21时，该过程相同，除了注射在颈部的左侧给予之外。在疫苗剂量确认记录(vaccinedoseconfirmationrecord)上，记录批号、剂量的量、动物标识号和疫苗材料的施用时机。
在疫苗接种期过程中，每天使用一般健康观察表对动物进行评估。具体地，如果所有动物都是正常的，则对于状态输入n。如果发现异常猪，则对于状态输入a，并且列出具体动物标识号和异常。每次疫苗接种后，监测注射部位区域的发红、肿胀、发热和疼痛的存在(或存在或不存在)和大小(cm)至少三天。如果病变显而易见，则监测它们直至消退。
在血液收集日，使用适当大小的
将血清样品保持在2-8℃下直至测试。加工在收到后48小时内完成。血液管在4℃下以1960xg离心10分钟。血清通过离心与凝块分开，并且倾析到两个螺旋盖低温小瓶内，所述小瓶标有至少研究编号、研究日和动物id。等分试样贮存于-70℃±10℃下。在这项研究完成后，样品贮存最少六个月。
使用sva分离株nac#20150909，通过病毒中和测定来测试选择的血清样品。
在测试结束时，将动物深度麻醉，并且从每只动物中收集1x250ml离心瓶的血液。对动物实施安乐死并且触诊注射部位。所有动物都按照aup和设施标准操作程序进行饲养。
猪被视为实验单位。母猪编号9、116、787和895(n＝30)出生的以及来自母猪编号141和145(n＝6)的可用猪列表用于随机化。具体地，对猪分配随机编号(使用random.org)。猪按母猪分类，然后为随机编号。围栏分配被分配随机编号(使用random.org)。然后将围栏分配按随机编号分类，并且与动物列表组合。
统计分析和数据概括由研究监测仪进行。所有数据都输入
内部开发的病毒中和测定用于测量疫苗接种后动物中的血清转化。在疫苗接种之前，没有动物具有可检测的血清学应答。到d35时，所有接种疫苗的动物(组1)都具有可检测的应答。该测定以1:80的稀释度运行，并且将动物视为阳性或阴性。关于svavn结果的概括，参见下表3。
表3：按组和研究日的svavn结果
这项研究的主要目的是评估在用sva疫苗的疫苗接种后，常规猪是否血清转化为sva。
关于sva疫苗，用灭活的sva原型疫苗接种疫苗的100％猪能够生成中和抗体应答。总之，这项研究能够证实原型灭活sva疫苗的功效的合理预期。
实施例2
在pucidt-amp载体(integrateddnatechnologies)中排序了两个基因序列。svvp13c基因(seqidno：18)是全长p1多蛋白的天然序列，具有连接p1多蛋白和3c自切割蛋白酶的2a以及部分2b和3b序列。svvp13c-co基因(seqidno：20)是svvp13c的昆虫密码子优化版本，其中p1、2a和2b序列具有使用idt密码子优化工具(idtcodonoptimizationtool)进行密码子优化，而3b和3c保留为天然序列。这两个基因均具有在起始密码子之前的kozak序列，以及分别在5'和3'末端处的bamhi和noti限制性位点。svvp13c(seqidno：18)和svvp13c-co(seqidno：20)插入片段分别通过bamhi和noti消化从pucidt-amp-svvp13c和pucidt-amp-svvp13c-co质粒中切下，并且连接到pvl1393载体(bdbiosciences)内。关于天然和密码子优化的pvl1393构建体的构建体设计，参见图1。
使用在每个基因的3'末端上添加关于6xhis标签的编码序列的引物(seqidno：34、35、36和37)进行pcr扩增，以分别从pidt-amp-svvp13c和pidt-amp-svvp13c-co扩增svvp1-his(seqidno：26)和svvp1co-his(seqidno：27)序列。使用引物从pidt-amp-svvp13c扩增svv3c序列(seqidno：30)，以添加5’spei位点和3’saci位点(seqid：38和39)。(关于引物见表4)。
表4.关于svvp1-his-svv3c和svvp1co-his-svv3c的引物序列
表5.关于svvp13cvp3/vp1和svvp13cd的引物序列
使用spei和saci限制性位点，将svv3c插入片段(seqidno：30)连接到porb-mcs1-sires-mcs2载体(allelebiotechnology)的第二个多克隆位点(mcs2)内。随后，利用bamhi和noti限制性位点，将svvp1-his或svvp1co-his连接到porb-mcs1-sires-svv3c的mcs1内，以分别生成porb-svvp1-his-sires-svv3c(seqidno：32)和porb-svvp1co-his-sires-svv3c(seqidno：33)。
引物设计为使pucidt-amp-svvp13c中的svvp13c序列(seqidno：18)的vp3/vp1切割位点(核苷酸1786-1797)从fh/st突变为pq/gv(关于引物参见上表5，seqidno：40和41)，使用lightningquiksitedirectedmutagenesis试剂盒(stratagene)。突变的序列，svvp13cvp3/vp1(seqidno：22)从pucidt-amp-svvp13cvp3/vp1中切下，并且连接到pvl1393载体内，以产生pvl1393-svvp13cvp3/vp1。使用重叠引物组(seqidno：34、42、43和44)，通过重叠延伸pcr(oe-pcr)，另外的构建体将svv3d编码序列(seqidno:45)与svvp13c序列(seqidno：18)的3'末端融合。使用bamhi和noti限制性位点，将所得到的pcr产物svvp13cd(seqidno：24)克隆到pvl1393内。(关于svvp13cvp3/vp1(seqidno：22)和svvp13cd构建体(seqidno：24)的图解，参见图3)。
根据制造商的说明书，使用escort转染试剂(sigmaaldrich)，将基于pvl1393的质粒中的重组svv衣壳构建体与flashbacultra(fbu)杆状病毒dna(oxfordexpressiontechnologies)一起共转染到sf9细胞内，而将基于porb的质粒与baculogold杆状病毒dna(bdbiosciences)一起共转染到sf9昆虫细胞内。收获来自转染的sf9细胞的细胞培养上清液，并且以1,000xg离心5分钟进行澄清，以使细胞碎片形成团块。收集澄清的上清液，进行0.2μm过滤，并且作为p1转染收获物贮存。sf9昆虫细胞用于生成p2原液，然后p2原液用于生成svv构建体的p3和p4扩增，用于sf+昆虫细胞中的蛋白质表达评估。收获杆状病毒感染的sf+细胞，并且在4℃下以10,000xg的速率澄清10分钟。每天取样杆状病毒感染的sf+细胞的培养物，以通过vi-cell分析监测总细胞/ml、活细胞/ml、活力百分比和细胞直径。当活力≤30％活力或活细胞≤1x106个细胞/ml时，收获扩增。每天收集另外1ml样品，以评估随着感染进展的蛋白质表达，并且如上所述进行加工。将收集的上清液和细胞团块样品贮存在-70℃下直至评估。
裂解昆虫细胞团块以分开可溶性和不溶性级分
将sf+昆虫细胞培养物样品离心，以使细胞形成团块，这之后去除培养基，并且将细胞团块冷冻直至裂解。将团块重悬浮于含有下述的裂解缓冲液中：在去离子水中的20mmtris、1％tritonx-100、用于加上his标签的蛋白质的蛋白酶抑制剂混合物(10μl/ml)和benzonase(250单位/ml)，其中ph为7.4。将重悬浮的昆虫细胞裂解产物涡旋10秒，在室温下温育5分钟，再次涡旋10秒，然后在4℃下以19,090xg离心10分钟，以使不溶性材料形成团块。将可溶性裂解产物吸取离开不溶性级分，并且在-70℃下贮存于管中。
svv重组衣壳蛋白的纯化
将含有表达的重组svv衣壳蛋白的上清液收获物进行0.2μm过滤，分配到超速离心管内，并且在4℃下以100,000xg离心2小时，以使蛋白质和可能的vlp形成团块。小心倾析澄清的上清液，然后将形成团块的材料重悬浮于tbs中，并且贮存于4℃下。不连续的10％-60％蔗糖梯度用于进一步纯化关于分别构建体的重组svv衣壳蛋白。将分别重悬浮的材料加入梯度的顶部，并且在4℃下以100,000xg离心2小时。将来自蔗糖梯度的级分平均收集到管内(其中级分1从梯度表面的顶部开始)，并且贮存于4℃。
sds-page和蛋白质
使用nupage电泳系统和4-12％bis-trismes微型凝胶进行sds-page。在还原条件下，使用0.05mdtt，在175v下适当的时间来分开样品。凝胶使用estain2.0proteinstainingdevice就总蛋白质进行染色，或使用iblot系统转移到硝酸纤维素膜用于蛋白质印迹。，通过snapidproteindetectionsystem(emdmillipore)，利用在抗体稀释物中的阴性对照杆状病毒抗原，用抗svv肽兔多克隆抗体(抗vp1-2、抗vp2-2和抗vp3-1，不同稀释度)和山羊抗兔过氧化物酶标记的二抗(1：500)进行蛋白质印迹，并且使用tmb膜过氧化物酶底物显色。
纯化的svv重组衣壳蛋白的透析和浓缩
将如通过蛋白质印迹测定的含有重组蛋白质的蔗糖梯度级分合并在一起，并且分配到10,000mwco或50,000mwco纤维素膜透析盒内。将透析盒置于具有磁力搅拌棒的3.5ltbs中，盖上盖子，并且在搅拌板上在4℃下放置最少6小时。然后将透析盒置于3.5ltbs的新鲜烧杯内，并且伴随搅拌进一步透析过夜或更长时间。从透析盒中取出样品，并且根据需要进行浓缩，取决于透析样品的体积。使用尺寸排阻过滤器单元进行浓缩，并且根据制造商的指导离心，直到达到所需的样品体积。
电子显微镜(em)成像
通过在usdanadcfacility处的透射电子显微镜检查(em)，评估蔗糖梯度纯化和透析的重组svv衣壳蛋白样品，用于vlp的可视化。关于天然svv的二十面体病毒衣壳的预期大小为直径大约27nm。
结果与讨论
天然svvp1多蛋白(seqidno：19，氨基酸1-859)由自切割蛋白酶加工，以形成个别的病毒衣壳亚基蛋白vp1-vp4。以类似于用于fmdv杆状病毒构建体的方式设计本发明中描述的svv杆状病毒构建体，利用表达的多蛋白自切割成个别的蛋白质亚基，用于形成空衣壳或vlp。用天然编码序列或对于昆虫细胞密码子优化的p1、2a和2b序列(seqidno：20)，来制备编码全长svvp1多蛋白、2a、部分2b和3b以及3c(seqidno：18)的构建体。用类似的基于pvl1393的fmdv杆状病毒构建体的先前研究揭示了归于3c表达的sf+细胞毒性。通过还生成基于porb的杆状病毒构建体用于评估，解决了关于潜在的svv3c毒性的关注。基于porb的构建体利用内部核糖体进入位点(wssvsires)(seqidno：32，核酸2647-2826)，允许起始帽不依赖性mrna翻译，其不同于与多角体蛋白启动子的3'-末端最邻近的序列的5'帽依赖性翻译。几项研究已显示，sires(例如来自wssv的那种)的使用已经常导致放置在sires之后的翻译序列的蛋白质表达降低。在所述svv杆状病毒构建体(seqidno：32和33)的背景下，sires的利用可以减少svv3c自切割蛋白酶的表达，其依次又可以缓解3c毒性问题，而不减少svvp1的表达。基于porb和基于pvl1393的构建体是相似的，因为它们均利用相同的svvp1序列，并且包括c末端6x-his标签。然而，基于porb或pvl1393的构建体之间的主要差异在于，基于porb的构建体将svv3c蛋白酶序列置于sires位点后面。在构建体的这些初始集合的评估后，又创建了修饰原始svvdna序列的两种构建体。一种构建体修饰vp3/vp1切割位点序列(seqidno：22)，而另一种构建体在svv3c序列(seqidno：24)的末端处进一步掺入了svv3ddna序列。评估所有构建体的衣壳亚基的表达以及vlp的产生。
svv衣壳蛋白在杆状病毒感染的昆虫细胞中的表达
baculofbu/svvp13c(seqidno：18)和baculofbu/svvp13c-co(seqidno：20)构建体用于感染sf+细胞，其中收集样品以评估svv衣壳蛋白的表达。在来自杆状病毒感染的昆虫细胞上清液中，通过用抗svvp1亚基特异性抗体的蛋白质印迹，检测关于vp1(seqidno：19，氨基酸596-859)、vp2(seqidno：19，氨基酸73-356)和vp3(seqidno：19，氨基酸357-595)衣壳亚基的预期大小的蛋白质条带，其大小与天然svv衣壳蛋白相似(图4)。有趣的是，在α-svvvp1和α-svvvp3蛋白质印迹中检测到～55kda的另外蛋白质条带。在天然svv抗原样品或阴性对照中未检测到～55kda条带(图4)。大小～55kda的另外蛋白质的存在提示它可能包含未切割的vp3-vp1蛋白质产物。
图4还提供了杆状病毒感染的sf+细胞中的天然(a)(seqidno：18)和密码子优化的(b)(seqidno：20)svvdna序列之间的svv衣壳蛋白表达水平的比较。基于这些蛋白质印迹，在密码子优化的baculofbu/svvp13c-co(seqidno：20)与原始的天然baculofbu/svvp13c(seqidno：18)之间，不存在svv衣壳蛋白表达水平中的明显差异，因此，仅进一步评估了baculofbu/svvp13c(seqidno：18)构建体。具有置于sires后面的3c蛋白酶的第二个构建体集合在sf+昆虫细胞的感染期间未产生可检测的svv衣壳亚基蛋白。sires构建体不仅没有产生可检测水平的svv衣壳亚单位蛋白，而且未观察到sf+细胞毒性问题。因而，没有进一步评估sires构建体。基于这些总体观察，关于baculofbu/svvp13c感染的sf+昆虫细胞的上清液收获物是最有希望的构建体，以前进至蔗糖梯度纯化和vlp存在的评估。
为了测定baculofbu/svvp13c衍生的svv衣壳蛋白在蔗糖梯度中的预期迁移率，将形成团块的天然svv病毒在蔗糖梯度上分开，并且通过蛋白质印迹进行分析(图5a)。在关于天然svv的蔗糖梯度纯化后，在级分5和6中检测到大多数天然svv亚基蛋白。预计如果vlp在杆状病毒感染的sf+细胞中形成，则它们将存在于相似或略微更高的收集梯度级分范围内。
来自baculofbu/svvp13c构建体的收获物上清液也进行加工用于蔗糖梯度纯化。蔗糖梯度级分的蛋白质印迹仅在蔗糖级分一中的一小部分重组svvvp2衣壳蛋白中检测到(图5b)。在蔗糖级分中未检测到vp1和vp3，尽管通过α-svvvp1蛋白质印迹，在蔗糖级分各处检测到在～55kda处的微弱蛋白质条带，被认为是未切割的vp3-vp1。与从天然svv中检测到的预期蛋白质形成对比，在级分5和6中未检测到重组svv衣壳蛋白。这些结果提示，在baculofbu/svvp13c感染的昆虫细胞中表达的衣壳亚基蛋白不形成vlp。
即使baculofbu/svvp13c(seqidno：18)产生用α-svv抗体作为亚基蛋白检测到的重组svv衣壳蛋白，但未观察到vlp。当与天然svv病毒蛋白相比较时，衣壳亚基蛋白具有相似的大小，但在杆状病毒感染的sf+细胞中也检测到几个其它蛋白质条带，包括在α-svvvp1和α-svvvp3蛋白质印迹中的～55kda处的蛋白质条带，提示天然svv病毒中不存在未切割的vp3-vp1蛋白产物。这可能指示关于vp3-vp1亚基的有效切割和分开的问题，其依次又可能阻碍杆状病毒感染的昆虫细胞中的vlp形成。
修饰的baculofbu/svvp13c构建体的评估
为了调查svv衣壳亚基蛋白vp1和vp3不能完全分开的可能性，基于先前的出版物设计了两种新的构建体。通过hales等人的2008年出版物陈述了svvp1vp3/vp1切割位点fh/st是非典型的微小核糖核酸病毒，包括与svv最密切相关的属，心病毒属。相比之下，pq/gv的典型切割位点在许多已知的微小核糖核酸病毒中是保守的。因此，vp3/vp1切割位点突变为典型的微小核糖核酸病毒切割序列可以增强vp3和vp1衣壳亚基的切割。使baculofbu/svvp13c(seqidno：18)构建体突变，以在vp3/vp1界面处含有pq/gv序列，导致指定为baculofbu/svvp13cvp3/vp1(seqidno：22)的构建体。这种新的构建体用于感染sf+细胞，并且以与baculofbu/svvp13c构建体(seqidno：18)相似的方式在蛋白质表达评价中进行评估。使用α-svvvp1或α-svvvp3抗体的baculofbu/svvp13cvp3/vp1衍生的上清液样品的蛋白质印迹分析，检测到推定的未切割的vp3-vp1蛋白产物、以及以与从baculofbu/svvp13c(seqidno：18)观察到的相同比例的vp3或vp1条带的个别亚基(图6)。在baculofbu/svvp13cvp3/vp1收获物上清液中也检测到在svvp1多蛋白的预期全长处的蛋白质条带，～95kda。
另外，收获物上清液的蔗糖梯度级分的蛋白质印迹评估(图7)与baculofbu/svvp13c(seqidno：18)的结果可比较。α-svvvp2蛋白质印迹检测到在蔗糖级分各处的重组svvvp2衣壳蛋白，其中大部分在级分一和九中。在蔗糖级分中未检测到亚基vp1和vp3蛋白，尽管在α-vp1蛋白的蔗糖级分各处、以及在α-vp3蛋白质的蔗糖级分一和九中检测到在～55kda处的蛋白质条带，其可能是vp3-vp1未切割的蛋白质。所有三个蛋白质都具有在最后一个蔗糖级分中检测到的几个蛋白质条带，其看起来与蔗糖梯度的起始样品相似。这提示svv衣壳蛋白可能聚集并沉淀到梯度的底部。衣壳蛋白在图5b中的baculofbu/svvp13c(seqidno：18)蔗糖梯度蛋白质的最后一个级分中未检测到，但在先前的评估中观察到(nb3219-123)。根据这些蛋白质评估看起来，切割序列从fh/st到pq/gv的突变对被视为未切割的vp3-vp1的～55kda条带的存在没有作用。与原始构建体相比，未检测到亚基vp3和vp1蛋白的量中的增加，并且也未观察到vlp形成。
当天然病毒中具有3cd蛋白酶时，在微小核糖核酸病毒内的其它属包括肠道病毒和口疮病毒已显示更有效的vp3-vp1切割；因此，设计了第二种塞内卡病毒构建体baculofbu/svvp13cd(seqidno：24)。baculofbu/svvp13cd(seqidno：24)在sf+细胞中表达，并且通过蛋白质印迹评估上清液收获物(图8)。观察到与先前的vlp评价可比较的结果，其中在α-svvvp1和α-svvvp3蛋白质印迹中检测到vp1、vp2和vp3亚基，以及～55kda的可疑的未切割的vp3-vp1蛋白。
与用其它svv杆状病毒构建体的svv蛋白质表达评价形成对比，关于baculofbu/svvp13cd(seqidno：24)的蔗糖级分的蛋白质印迹评估显示出存在于蔗糖级分1和2中的单体svvvp1、vp2和vp3衣壳亚基蛋白，其中在蔗糖级分各处检测到vp1和vp2。～55kda蛋白质条带存在于蔗糖级分1和2中的α-svvvp1和α-svvvp3蛋白质印迹中，但不存在于阴性对照样品或α-svvvp2蛋白质印迹中(图9)。类似于baculofbu/svvp13cvp3/vp1(seqidno：22)蔗糖级分评估，大部分svv衣壳蛋白聚集并沉淀到关于baculofbu/svvp13cd(seqidno：24)的蔗糖梯度底部。然而，在衍生自baculofbu/svvp13cd感染的sf+细胞构建体的级分各处检测到svvvp1和vp2亚基蛋白提示了vlp形成的可能性。
尽管来自每种构建体的蔗糖梯度评估的不明确结果，仍通过在usdanadc处的电子显微镜检查(em)评估预期含有vlp的级分。来自上清液收获物baculofbu/svvp13c(seqidno:18)、baculofbu/svvp13cvp3/vp1(seqidno:22)和baculofbu/svvp13cd(seqidno:24)的合并的蔗糖级分在tbs中进行透析，并且在制剂中进行浓缩用于em成像。样品具有较高的背景，使得难以通过em阴性染色明确地描绘vlp。在baculofbu/svvp13cvp3/vp1(seqidno:22)和baculofbu/svvp13cd(seqidno:24)合并的蔗糖级分中稀疏地可见一些球形，其大小与预期的svvvlp相似，但在样品各处不存在足够的球形以确认vlp的存在。
重组svv蛋白在sf+昆虫细胞内部表达
关于为何在上清液中检测到个别重组svv病毒衣壳蛋白，但看起来没有形成vlp的一种可能性是，由于昆虫细胞培养基的低ph，vlp在释放到上清液内之后不久可能解离。为了测试这一假设，来自baculofbu/svvp13c(seqidno:18)、baculofbu/svvp13cvp3/vp1(seqidno:22)和baculofbu/svvp13cd(seqidno:24)感染的第3天(当细胞的活力仍相对较高时)的细胞团块样品，在生理ph缓冲液中裂解，以获得可溶性蛋白质级分，用于通过蛋白质印迹评估重组svv亚基蛋白(图10)。如用杆状svv构建体的上清液收获物样品可见的，在第3天的可溶性级分中存在可检测水平的亚基衣壳蛋白vp1、vp2和vp3。如先前观察到的，在杆状svv样品泳道中的α-svvvp1和α-svvvp3蛋白质中，也检测到疑为未切割的vp3-vp1蛋白的～55kda条带。第3天可溶性级分样品进行蔗糖梯度纯化，并且通过蛋白质印迹进行评估，以观察vlp是否存在于裂解前的细胞中(图11)。
如分别用来自由baculofbu/svvp13c(seqidno:18)、baculofbu/svvp13cvp3/vp1(seqidno:22)和baculofbu/svvp13cd(seqidno:24)感染的昆虫细胞的上清液收获物蔗糖级分可见的，来自第3天可溶性级分的蔗糖级分具有可比较的结果。预期为svv衣壳蛋白的蛋白条带大部分在级分一和二中检测到和/或沉淀到底部，提示昆虫细胞内部的svv亚基蛋白在细胞裂解前不形成vlp。对baculofbu/svvp13cvp3/vp1(seqidno：22)第3天可溶性样品的合并的蔗糖级分进行em，其中对于这种样品未观察到vlp。baculofbu/svvp13c(seqidno：18)和baculofbu/svvp13cd(seqidno：24)第3天可溶性蔗糖级分未通过em进行评估。
结论
尽管测定重组svv病毒衣壳蛋白vp1、vp2和vp3在某种程度上被表达为完全切割的蛋白质，但基于蔗糖梯度纯化和em成像，它们并未导致vlp形成。在蔗糖梯度级分的蛋白质印迹评估中，在梯度的前几个级分和/或最后一个级分中检测到重组svv衣壳蛋白。这些结果提示，蛋白质并不组装成vlp，而是作为非结合单体保留或形成大聚集体，指示错误折叠或错误组装的蛋白质。电子显微镜检查支持这些结果，因为在含有svv衣壳亚基蛋白的合并的蔗糖级分中未检测到vlp。
实施例3
这项研究利用常规动物来测定疫苗施用后的血清学应答诱导的初步可行性。这项研究的主要目的是评估原型疫苗baculofbu/svvp13c(seqidno:18)、baculofbu/svvp13cvp3/vp1(seqidno:22)和baculofbu/svvp13cd(seqidno:24)的施用是否导致在常规猪中的血清转化。
如表1中所示，将四十头猪随机分成四组。关于组的描述和住房结构，参见下表6。在d0时，向猪施用2ml肌内剂量的原型疫苗或安慰剂。在第21时，动物接受原型疫苗或安慰剂的加强施用。在每次疫苗接种(d0和d21)和d35时的治疗施用之前，从所有猪中收集血液。就血清转化为sva的证据测定血清样品的子集。在研究自始至终记录一般健康观察。在疫苗施用后观察注射部位的反应最少三天。在试验结束时对动物实施人道安乐死。关于关键事件和样品收集的时间表，参见表7。
表6：研究设计
表7.关键事件和样品收集的时间表
*注意观察持续直到反应消退
为了避免偏差，通过不涉及动物临床监测的人员在d0和d21时施用治疗。在d0时，使用适当大小的无菌针头和注射器，将2ml剂量的疫苗施用于健康猪的右颈部的肌肉组织内。在d21时，该过程相同，除了注射在颈部的左侧给予之外。在疫苗剂量确认记录(vaccinedoseconfirmationrecord)上，记录批号、剂量的量、动物标识号和疫苗材料的施用时机。
在疫苗接种期过程中，每天使用一般健康观察表对动物进行评估。具体地，如果所有动物都是正常的，则对于状态输入n。如果发现异常猪，则对于状态输入a，并且列出具体动物标识号和异常。每次疫苗接种后，监测注射部位区域的发红、肿胀、发热和疼痛的存在(或存在或不存在)和大小(cm)至少三天。如果病变显而易见，则监测它们直至消退。
在血液收集日，使用适当大小的
将血清样品保持在2-8℃下直至测试。加工在收到后48小时内完成。血液管在4℃下以1960xg离心10分钟。血清通过离心与凝块分开，并且倾析到两个螺旋盖低温小瓶内，所述小瓶标有至少研究编号、研究日和动物id。等分试样贮存于-70℃±10℃下。在这项研究完成后，样品贮存最少六个月。
实施例4
这项研究利用常规动物来评估两剂量的塞内卡病毒a疫苗、灭活的全病毒针对异源塞内卡病毒a野外分离株的异源攻击的功效。总共25头猪用于该研究。将动物随机分成两个治疗组。在第d0天时，sva-vx组中的十三头猪用塞内卡病毒a疫苗(灭活的全病毒)进行肌内(im)接种，而安慰剂组中的剩余十二头猪接受对照产品。在第14天时，使用适当的材料，对所有猪肌内施用加强疫苗。在d35时，所有猪都用总体积为5ml(经口2ml和鼻内3ml)的8.36logtcid50/剂量的异源塞内卡病毒a野外分离株(病毒收获物)进行攻击。所有的猪都混合饲养在一个房间里。从d0到d33每天监测猪的一般健康。从d34到d49，每天监测猪的与sva感染相关的临床体征。在研究自始至终定期获取血液和直肠温度。在d49(攻击后14天)时，对所有动物进行尸检。关于实验设计概括，参见表8。
表8.研究设计
关于疫苗和对照产品制剂的概括，参见下表9。对疫苗材料进行常规培养和支原体属物种(mycoplasmasp.)pcr；未检测到生长(血琼脂上的厌氧或好氧)或支原体dna污染。使用适当大小的无菌针和注射器，在d0时，将疫苗肌内施用到颈部的右侧内，在耳根与肩点之间的中间位置。在第14天时，疫苗在与先前所述相同的位置中施用，但在颈部的左侧上。所有组都接受2ml剂量。
表9.疫苗和对照
基于来自野外病例和先前出版物的报道，监测动物的跛行、蹄病变和水泡的存在。如果动物在研究自始至终具有任何临床异常，则它被视为受影响的。表10按组报告了受影响动物的频率。预防分数估计值0.322(0.004，0.539；95％ci)指示疫苗接种减少受影响动物的数目。还对动物表展示异常临床体征的天数完成缓解分数分析。缓解分数估计值0.710(0.333，0.935；95％ci)指示疫苗接种减少动物视为受影响的天数。
表10.在攻击期过程中按组的临床体征存在/不存在的频率分布
*受影响＝临床体征在研究期间观察到至少一次
在攻击期过程中收集直肠温度；图12中呈现了按研究日和组的最小均方直肠温度。疫苗接种导致在d40和42时显著降低的温度。
通过病毒中和测定评估来自d0、14、35和49的血清样品。表11中呈现了按组和研究日的几何平均滴度。在两个剂量的疫苗后，13/13(100％)个动物具有大于400的中和滴度。
表11.关于按研究日和组的病毒中和滴度的概括统计。<40的值报告为20；>2560的值设为2560。
通过qrt-pcr测定检测血清中病毒rna的存在。图13展示了血清中svarna的组中值数量(log10基因组拷贝/ml)。在研究期间的任何时间，在接种疫苗的动物中未检测到svarna。相比之下，从d36直到d42，在安慰剂组中检测到病毒血症。
总之，用两个剂量的塞内卡病毒a(灭活的全病毒)的疫苗接种导致病毒血症的完全减少、临床体征中的统计学显著减少(pf＝0.322；95％ci＝0.004，0.539)、以及在13/13个疫苗接种的动物中攻击前4倍的病毒中和滴度。
根据本公开内容，无需过度实验即可制备且实施本文公开且请求保护的所有组合物和方法。尽管本发明的组合物和方法已根据优选实施方案进行描述，但对于本领域技术人员而言显而易见的是，可以对组合物和方法以及在本文中描述的方法的步骤或步骤顺序应用变化，而不背离本发明的概念、精神和范围。更具体地，显而易见的是，化学和生理学相关的某些试剂可以取代本文所述的试剂，同时达到相同或相似的结果。对于本领域技术人员显而易见的所有此类相似的取代和修改都视为在如由下述权利要求限定的本发明的精神，范围和概念内。
序列表
<110>勃林格殷格翰动物保健美国有限公司
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gguguguugugugccuacguugaagacccgaccaaaucugacccuccguccagcagcaca600
gaucaacccaccaccacuuuuacugccaucgacaggugguacacuggacgccucaauucu660
uggacaaaagcuguaaaaaccuucucuuuucaggccguuccgcucccuggagccuuccug720
ucuagacagggaggccucaacggaggggccuucacggccacccuacauagacauuucuua780
augaagugcggguggcaggugcagguucaaugcaauuugacgcaauuccaccaaggugcu840
cuucuuguugccaugguccccgaaaccacccuugaugucaaaccugacggcaaggcaaag900
agcuuacaggagcugaaugaagagcaguggguggaaaugucugacgacuaccggaccggg960
aaaaacaugccuuuucagucucuuggcacauacuaucggcccccuaacuggacuuggggc1020
cccaauuuuauuaaccccuaucaaguaacagucuucccacaccaaauucugaacgcgaga1080
accucuaccucgguagacauaagugucccguacaucggggagacuccuacacaauccuca1140
gagacacagaacuccuggacccuccuuguuauggugcuugucccccuggacuacaaggag1200
ggagccacaacugacccagaaauuacauuuucuguaaggccuacaagucccuacuucaau1260
gggcuucguaaccguuucaagaccgggacggacgaggaacaggggcccauucccacagca1320
cccagagaaaauucgcuuauguuucucucaaccaucccugacgacacugucccugcuuac1380
gggaaugugcguaccccucccgucaauuaccuccccggugaaauaaccgaccucuuacaa1440
cuggcccguauacccacucucauggcguuugggcgggcgucugaacccgagccugccuca1500
gacgcauaugugcccuacguugccguuccugcccaguucgacgacaagccucucaucucc1560
uucccgaucacccuuucagauccugucuaccagaacacccugguaggcgccaucaguucg1620
aacuucgccaacuaccggggguguauccaaaucacucugacauuuuguggacccaugaug1680
gcaagagggaaauuccugcucucguauucucccccaaauggagcacaaccacagacccuu1740
ucugaagcuaugcagugcacauacucuauuugggauauaggcuugaacucuaguuggacc1800
uuugucauccccuacaucucgcccagugauuaccgugaaacucgggcuauuaccaacuca1860
guuuauucugcugaugguugguuuagcuugcacaagcugaccaaaauuacucuaccaccu1920
gacugcccacagagucccuguauucucuuuuucgccucugcuggugaggauuacacccuc1980
cgucucccuguugauuguaauccuuccuacguguuccacuccaccgacaacgccgagacu2040
gggguuauugaggcagguaacacugacaccgauuucucuggugaacuggcggcuccuggc2100
ucuaaccauacuaaugucaaauuccuguuugaccgaucucgacuacugaauguaauuaag2160
guacuggagaaggacgccgucuucccccguccuuuccccacagcaacaggugcacagcag2220
gacgaugguuacuuuugucuucuaacaccccgcccaacagucgcuucccgacccgccacu2280
cguuucggccuguacgucaacccgucugacaguggcguucucgcuaacacuucacuggau2340
uucaauuuuuacaguuuggccuguuucacuuacuuuagaucagaccuugaagucacggug2400
gucucacuggagccagauuuggaguucgccguggggugguuccccucuggcagugaguac2460
caggcuucuagcuuugucuacgaccaacugcauguacccuaccacuuuacugggcgcacu2520
ccccgcgcuuucaccagcaaggguggaaagguaucuuucgugcucccuuggaacucuguc2580
ucuuccgugcuucccgugcgcugggggggcgccuccaagcuuucuucugccacgcggggu2640
cugccggcucaugcugacugggggaccauuuacgccuuuaucccccgucccaacgagaag2700
aaaagcaccgcuguaaagcacguggcgguguacguucgguacaagaacgcgcgugccugg2760
ugccccagcaugcuucccuuucgcagcuacaagcagaagaugcugaugcaaucaggcgac2820
gucgagaccaacccuggcccugcuucugacaacccgaucuuggaguuucuugaagcggaa2880
aacgaucuagucacucuggccucucucuggaagaugguacacucuguucaacagaccugg2940
agaaaguaugugaagaacgacaauuuuuggcccaacuugcucagugagcuagugggggaa3000
ggcuccaucgccuuggccgccacgcuaucuaaccaagcuucagugaaagcucucuugggc3060
cugcauuuucucucucgagggcucaauuacacagauuuuuacucuuuacugauagagaaa3120
ugcucuaguuucuuuacuguagaaccgccuccuccaccagcugaaaaucugaugaccaag3180
cccuccgugaagucgaaauuccgaaagcuguuuaagaugcaaggacccauggacacaguc3240
aaagacuggaaccaaauagccgccggcuugaagaauuuccaauuuguucgugaccuaguc3300
aaagaggugguugacuggcuccaggccuggaucaauaaagagaaagccagcccuguccuc3360
caguaccagcuggagaugaagaagcucgggcccguggcuuuggcucaugaugccuucaug3420
gccgguuccgggcccccucuuggugacgaccagauugaauaccuccagaaccucaaaucu3480
cuugcccuaacacugggaaagacuaauuuggcccaaagucucaccacuaugaucaacgcc3540
aagcagagcuccgcccaacgagucgaacccguuguggugguccucagaggcaagccggga3600
ugcggcaaaagcuuggccuccacguugauugcccaggcugugucuaagcgucucuacggc3660
ucacagaguguguauucucuuccuccggacccagacuucuucgacggauauaaaggacag3720
uuuguaaccuugauggacgaucugggacaaaacccggaugggcaagauuucuccaccuuu3780
ugucagauggugucgaccgcccaauuucuucccaacauggcggaccuugcagagaagggg3840
cgucccuucaccuccaaucuuaucauugcaacuacaaaccucccucacuuuagcccuguc3900
accauugcugacccuucugcagucucucggcguaucaacuacgaccugacucuagaagua3960
ucugaggccuacaagaagcacacgcggcugaauuucgaccuggcuuucagacgcacugac4020
gccccccccauuuauccuuuugcugcccaugugcccuucguggacguggcugugcgcuuc4080
aaaaauggucaucaaagcuucaaucuccuggaguuggucgacucuauuugugcagacauu4140
cgggccaagcaacaaggugcccgaaauaugcagacucugguucuacagagcccuaacgag4200
aacgacgacacccccgucgacgaggcguuggguagaguucucacccccgcugcggucgac4260
gaggcgcuugucgaccucgcuccagaugccgacccgguuggccgcuuagcuauucucgcc4320
aagcuaggucuugcccuagcugcggucacuccugguuugauaaucuuggcagugggacuc4380
uacaaguacuucucuggcucugauacagaccaagaagaaacagaaagugaggagccugcu4440
aaagugccuaggagcgagaaugcuuaugacggcccaaagaaaaacuccaagcccccugga4500
gcgcucucucuuauggaaaugcaacagcccaacguggacaugggcuuugaggcugcaguu4560
gcuaagaaaguggucguccccauuaccuucaugguucccaacagaccuuccggacuuaca4620
caguccgcucuucuuguggccggccggaccuuccuaaucaaugagcauacaugguccaac4680
cccuccuggaccagcuucacaauccguggugaggugcacacucgugaugagcccuuccaa4740
acgguucauuuuacucaccauggucuucccacagaucugaugaugguacgucucggaccg4800
ggcaacucuuucccuaacaaucuagauaaguuuggacuugaccagaugccggcacguaac4860
ucccgugugguuggcguuucggcuaguuacgguaacuucuucuucucugggaacuuccuc4920
ggguuuguugacuccaucaccucugaccaaggaaccuaugcgagacuuuucagguacagg4980
gugacgacuuacaagggauggugcgguucggcccuggucugugaggccggugguguccga5040
cgcaucauuggcaugcauucugcuggugccgcugguaucggcgccgggacuuacaucuca5100
aaauuaggacugaucaaagcccuuaaacaccucggugagccucuggcuacaaugcaagga5160
cugaugacugagcuagagccuggagucaccguacauguaccccgaaaaucuaaauugaga5220
aagacgaccgcacacgcgguguacaaaccggaguuugaaccugcuguguugucaaaauuu5280
gaucccagacugaacaaggauguugaccuagaugagguaauuuggucuaaacacaccgcc5340
aacgucccuuaucaaccuccuuuguucuacacauacaugucagaguacgcucaucggguu5400
uucuccuuuuugggaaaagacaaugacauucugaccgucaaagaagcaauccugggcauc5460
ccuggacuagacccuauggauccccacacagcuccggguuugcccuacgccauuagcggc5520
cuucgacguacugaucucgucgauuuugcgaacggcacgguagacccggcacuggccaug5580
cagauccagaaauucuuagacggugacuacucugaucaugucuuccaaacuuuucugaaa5640
gaugaaaucagacccucagagaagguccgggcgggaaaaacccgcauugucgaugugccc5700
ucccuggcgcacugcauugugggcagaaugcugcuugggcgcuuugccgccaaguuucaa5760
ucccauccuggcuuucuccuuggcuccgcuaucgggucugacccugaugucuucuggacc5820
gucauaggggcucagcucgagggaagaaagaacacguaugacguggacuacagugccuuu5880
gacucuucacacggcacuggcuccuucgaggcucucaucucucacuuuuucaccguggac5940
aaugguuucagcccugcgcugggaccguaucucagaucccuggcugucucggugcacgcu6000
uacggcgagcgucgcaucaagauuaccggaggccuccccucugguugugccgcgaccagc6060
cugcugaacacagugcucaacaaugugaucaucaggacugcucuggcauugaccuacaag6120
gaauuugaauaugacaugguugauaucaucgccuacggugacgaccuucugguugguacg6180
gauuaugaucuggacuucaaugagguggcgcggcgcgcugccaaacugggguauaagaug6240
acuccugccaacaaggguucugucuucccuccgacuuccucucucuccgaugcuguuuuu6300
cuaaaacgcaaauucguccaaaacaaugacggcuuauauaaaccaguuauggauuuaaag6360
aauuuggaagccaugcucuccuacuucaaaccaggaacacuacucgagaagcugcaaucu6420
guuucuauguuggcucaacauucuggaaaagaagaauacgauagauugaugcaccccuuc6480
gcugacuauggugccguaccgagucacgaguaccugcaggcaagauggagggccuuguuc6540
gacuga6546
<210>3
<211>2181
<212>prt
<213>塞内卡病毒
<400>3
metglnasnserhispheserpheaspthralaserglythrpheglu
151015
aspvalthrglythrlysvallysilevalgluhisproargserval
202530
asnasnglyvaltyraspserserthrhisleugluileleuasnleu
354045
glnglygluilegluileleulysserpheasnglutyrglnilearg
505560
alaalalysglnglnleuglyleuaspilevaltyrgluleuglngly
65707580
asnvalglnthrthrserlysasnasppheaspserargglyasnasn
859095
glyasnmetthrpheasntyrtyralaasnthrtyrglnasnserval
100105110
asppheserthrserserseralaserglyalaglyproglyasnser
115120125
argglyglyleualaglyleuleuthrasnpheserglyileleuasn
130135140
proleuglytyrleulysasphisasnthrgluglumetgluasnser
145150155160
alaaspargvalilethrglnthralaglyasnthralaileasnthr
165170175
glnserserleuglyvalleucysalatyrvalgluaspprothrlys
180185190
seraspproproserserserthraspglnprothrthrthrphethr
195200205
alaileaspargtrptyrthrglyargleuasnsertrpthrlysala
210215220
vallysthrpheserpheglnalavalproleuproglyalapheleu
225230235240
serargglnglyglyleuasnglyglyalaphethralathrleuhis
245250255
arghispheleumetlyscysglytrpglnvalglnvalglncysasn
260265270
leuthrglnphehisglnglyalaleuleuvalalametvalproglu
275280285
thrthrleuaspvallysproaspglylysalalysserleuglnglu
290295300
leuasnglugluglntrpvalglumetseraspasptyrargthrgly
305310315320
lysasnmetpropheglnserleuglythrtyrtyrargproproasn
325330335
trpthrtrpglyproasnpheileasnprotyrglnvalthrvalphe
340345350
prohisglnileleuasnalaargthrserthrservalaspileser
355360365
valprotyrileglygluthrprothrglnsersergluthrglnasn
370375380
sertrpthrleuleuvalmetvalleuvalproleuasptyrlysglu
385390395400
glyalathrthraspprogluilethrpheservalargprothrser
405410415
protyrpheasnglyleuargasnargphelysthrglythraspglu
420425430
gluglnglyproileprothralaproarggluasnserleumetphe
435440445
leuserthrileproaspaspthrvalproalatyrglyasnvalarg
450455460
thrproprovalasntyrleuproglygluilethraspleuleugln
465470475480
leualaargileprothrleumetalapheglyargalaserglupro
485490495
gluproalaseraspalatyrvalprotyrvalalavalproalagln
500505510
pheaspasplysproleuileserpheproilethrleuserasppro
515520525
valtyrglnasnthrleuvalglyalaileserserasnphealaasn
530535540
tyrargglycysileglnilethrleuthrphecysglyprometmet
545550555560
alaargglylyspheleuleusertyrserproproasnglyalagln
565570575
proglnthrleuserglualametglncysthrtyrseriletrpasp
580585590
ileglyleuasnsersertrpthrphevalileprotyrileserpro
595600605
serasptyrarggluthrargalailethrasnservaltyrserala
610615620
aspglytrppheserleuhislysleuthrlysilethrleupropro
625630635640
aspcysproglnserprocysileleuphephealaseralaglyglu
645650655
asptyrthrleuargleuprovalaspcysasnprosertyrvalphe
660665670
hisserthraspasnalagluthrglyvalileglualaglyasnthr
675680685
aspthrasppheserglygluleualaalaproglyserasnhisthr
690695700
asnvallyspheleupheaspargserargleuleuasnvalilelys
705710715720
valleuglulysaspalavalpheproargpropheprothralathr
725730735
glyalaglnglnaspaspglytyrphecysleuleuthrproargpro
740745750
thrvalalaserargproalathrargpheglyleutyrvalasnpro
755760765
seraspserglyvalleualaasnthrserleuasppheasnphetyr
770775780
serleualacysphethrtyrpheargseraspleugluvalthrval
785790795800
valserleugluproaspleugluphealavalglytrppheproser
805810815
glyserglutyrglnalaserserphevaltyraspglnleuhisval
820825830
protyrhisphethrglyargthrproargalaphethrserlysgly
835840845
glylysvalserphevalleuprotrpasnservalserservalleu
850855860
provalargtrpglyglyalaserlysleuserseralathrarggly
865870875880
leuproalahisalaasptrpglythriletyralapheileproarg
885890895
proasnglulyslysserthralavallyshisvalalavaltyrval
900905910
argtyrlysasnalaargalatrpcysprosermetleuprophearg
915920925
sertyrlysglnlysmetleumetglnserglyaspvalgluthrasn
930935940
proglyproalaseraspasnproileleuglupheleuglualaglu
945950955960
asnaspleuvalthrleualaserleutrplysmetvalhisserval
965970975
glnglnthrtrparglystyrvallysasnaspasnphetrpproasn
980985990
leuleusergluleuvalglygluglyserilealaleualaalathr
99510001005
leuserasnglnalaservallysalaleuleuglyleuhisphe
101010151020
leuserargglyleuasntyrthraspphetyrserleuleuile
102510301035
glulyscysserserphephethrvalglupropropropropro
104010451050
alagluasnleumetthrlysproservallysserlysphearg
105510601065
lysleuphelysmetglnglyprometaspthrvallysasptrp
107010751080
asnglnilealaalaglyleulysasnpheglnphevalargasp
108510901095
leuvallysgluvalvalasptrpleuglnalatrpileasnlys
110011051110
glulysalaserprovalleuglntyrglnleuglumetlyslys
111511201125
leuglyprovalalaleualahisaspalaphemetalaglyser
113011351140
glyproproleuglyaspaspglnileglutyrleuglnasnleu
114511501155
lysserleualaleuthrleuglylysthrasnleualaglnser
116011651170
leuthrthrmetileasnalalysglnserseralaglnargval
117511801185
gluprovalvalvalvalleuargglylysproglycysglylys
119011951200
serleualaserthrleuilealaglnalavalserlysargleu
120512101215
tyrglyserglnservaltyrserleuproproaspproaspphe
122012251230
pheaspglytyrlysglyglnphevalthrleumetaspaspleu
123512401245
glyglnasnproaspglyglnasppheserthrphecysglnmet
125012551260
valserthralaglnpheleuproasnmetalaaspleualaglu
126512701275
lysglyargprophethrserasnleuileilealathrthrasn
128012851290
leuprohispheserprovalthrilealaaspproseralaval
129513001305
serargargileasntyraspleuthrleugluvalsergluala
131013151320
tyrlyslyshisthrargleuasnpheaspleualapheargarg
132513301335
thraspalaproproiletyrprophealaalahisvalprophe
134013451350
valaspvalalavalargphelysasnglyhisglnserpheasn
135513601365
leuleugluleuvalaspserilecysalaaspileargalalys
137013751380
glnglnglyalaargasnmetglnthrleuvalleuglnserpro
138513901395
asngluasnaspaspthrprovalaspglualaleuglyargval
140014051410
leuthrproalaalavalaspglualaleuvalaspleualapro
141514201425
aspalaaspprovalglyargleualaileleualalysleugly
143014351440
leualaleualaalavalthrproglyleuileileleualaval
144514501455
glyleutyrlystyrpheserglyseraspthraspglngluglu
146014651470
thrgluserglugluproalalysvalproargsergluasnala
147514801485
tyraspglyprolyslysasnserlysproproglyalaleuser
149014951500
leumetglumetglnglnproasnvalaspmetglyphegluala
150515101515
alavalalalyslysvalvalvalproilethrphemetvalpro
152015251530
asnargproserglyleuthrglnseralaleuleuvalalagly
153515401545
argthrpheleuileasngluhisthrtrpserasnprosertrp
155015551560
thrserphethrileargglygluvalhisthrargaspglupro
156515701575
pheglnthrvalhisphethrhishisglyleuprothraspleu
158015851590
metmetvalargleuglyproglyasnserpheproasnasnleu
159516001605
asplyspheglyleuaspglnmetproalaargasnserargval
161016151620
valglyvalseralasertyrglyasnphephepheserglyasn
162516301635
pheleuglyphevalaspserilethrseraspglnglythrtyr
164016451650
alaargleupheargtyrargvalthrthrtyrlysglytrpcys
165516601665
glyseralaleuvalcysglualaglyglyvalargargileile
167016751680
glymethisseralaglyalaalaglyileglyalaglythrtyr
168516901695
ileserlysleuglyleuilelysalaleulyshisleuglyglu
170017051710
proleualathrmetglnglyleumetthrgluleugluprogly
171517201725
valthrvalhisvalproarglysserlysleuarglysthrthr
173017351740
alahisalavaltyrlysprogluphegluproalavalleuser
174517501755
lyspheaspproargleuasnlysaspvalaspleuaspgluval
176017651770
iletrpserlyshisthralaasnvalprotyrglnproproleu
177517801785
phetyrthrtyrmetserglutyralahisargvalpheserphe
179017951800
leuglylysaspasnaspileleuthrvallysglualaileleu
180518101815
glyileproglyleuaspprometaspprohisthralaprogly
182018251830
leuprotyralaileserglyleuargargthraspleuvalasp
183518401845
phealaasnglythrvalaspproalaleualametglnilegln
185018551860
lyspheleuaspglyasptyrserasphisvalpheglnthrphe
186518701875
leulysaspgluileargproserglulysvalargalaglylys
188018851890
thrargilevalaspvalproserleualahiscysilevalgly
189519001905
argmetleuleuglyargphealaalalyspheglnserhispro
191019151920
glypheleuleuglyseralaileglyseraspproaspvalphe
192519301935
trpthrvalileglyalaglnleugluglyarglysasnthrtyr
194019451950
aspvalasptyrseralapheaspserserhisglythrglyser
195519601965
pheglualaleuileserhisphephethrvalaspasnglyphe
197019751980
serproalaleuglyprotyrleuargserleualavalserval
198519901995
hisalatyrglygluargargilelysilethrglyglyleupro
200020052010
serglycysalaalathrserleuleuasnthrvalleuasnasn
201520202025
valileileargthralaleualaleuthrtyrlysglupheglu
203020352040
tyraspmetvalaspileilealatyrglyaspaspleuleuval
204520502055
glythrasptyraspleuasppheasngluvalalaargargala
206020652070
alalysleuglytyrlysmetthrproalaasnlysglyserval
207520802085
pheproprothrserserleuseraspalavalpheleulysarg
209020952100
lysphevalglnasnasnaspglyleutyrlysprovalmetasp
210521102115
leulysasnleuglualametleusertyrphelysproglythr
212021252130
leuleuglulysleuglnservalsermetleualaglnhisser
213521402145
glylysgluglutyraspargleumethisprophealaasptyr
215021552160
glyalavalproserhisglutyrleuglnalaargtrpargala
216521702175
leupheasp
2180
<210>4
<211>7300
<212>rna
<213>塞内卡病毒
<400>4
uuugaaauggggggcugggcccucaugcccaguccuuccuuuccccuuccgggggguaaa60
ccggcuguguuugcuagaggcacagaggagcaacauccaaccugcucuuguggggaacgg120
ugcggcuccaauuccugcgucgccaaagguguuagcgcacccaaacggcgcaucuaccaa180
ugcuauugguguggucugcgaguucuagccuacucguuucuccccuauccacucacucac240
gcacaaaaaguguguuguaacuacaagacuuagcccucgcacgagaugugcgauaaccgc300
aagauugacucaagcgcggaaagcgcuguaaccacaugcuguuagucccuuuauggcugc360
gagauggcuauccaccucggaucacugaacuggagcucgacccuccuuaguaagggaacc420
gagaggccuucuugcaacaagcuccgacacagaguccacgugauugcuaccaccaugagu480
acaugguucuccccucucgacccaggacuucuuuuugaauauccacggcucgauccagag540
gguggggcaugaucccccuagcauagcgagcuacagcgggaacuguagcuaggccuuagc600
gugcuuuggauacugccugauagggcgacggccuagucgugucgguucuauagguagcac660
auacaaauaugcagaacucucauuuuucuuucgauacagccucuggcaccuuugaagacg720
uaaccggaacaaaagucaagaucguugaauacccuagaucggugaacaaugguguuuacg780
auucguccacucauuuagagauacugaaccuacagggugaaauugaaauuuuaaggucuu840
ucaaugaauaccaaauucgcgccgccaaacaacaacuuggacuggacaucguauaugaac900
uacaggguaauguucagacaaccucaaagaaugauuuugauucccgcggcaauaauggua960
acaugaccuucaauuacuacgcaaacacuuaucagaauucaguagacuucucgaccuccu1020
cgucggcgucaggcgccggacccgggaacucccggggcggauuagcgggucuccucacaa1080
auuucaguggaaucuugaacccucuuggcuaccucaaagaccacaauaccgaagaaaugg1140
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cuaacgagaaugaugacacccccgucgacgaggcgcuggguagaguucucacucccgcug4920
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ccccuggagcgcucucucuuauggaaaugcaacagcccaacguggacaugggcuuugagg5220
cugcggucgcuaagaaaguggucguccccauuacauucaugguucccaacagaccuucug5280
gacuuacacaguccgcccuucuuguggccggccggaccuuccuaauuaaugagcauacau5340
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gccccuucgcugacuacggugccguaccgagucacgaguaccugcaggcaagauggaggg7200
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acucagucggaacagaaaagggaaaaaaaaaaaaaaaaaa7300
<210>5
<211>7286
<212>rna
<213>塞内卡病毒
<400>5
uuugaaauggggggcugggcccucaugcccaguccuuccuuuccccuuccgggggguaaa60
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ugcuauugguguggucugcgaguucuagccuacucguuucuccccuauccacucacucac240
gcacaaaaaguguguuguaacuacaagacuuagcccucgcacgagaugugcgauaaccgc300
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gagauggcuauccaccucggaucacugaacuggagcucgacccuccuuaguaagggaacc420
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acaugguucuccccucucgacccaggacuucuuuuugaauauccacggcucgauccagag540
gguggggcaugaucccccuagcauagcgagcuacagcgggaacuguagcuaggccuuagc600
gugcuuuggauacugccugauagggcgacggccuagucgugucgguucuauagguagcac660
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uaaccggaacaaaagucaagaucguugaauacccuagaucggugaacaaugguguuuacg780
auucguccacucauuuagagauacugaaccuacagggugaaauugaaauuuuaaggucuu840
ucaaugaauaccaaauucgcgccgccaaacaacaacuuggacuggacaucguauaugaac900
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acaugaccuucaauuacuacgcaaacacuuaucagaauucaguagacuucucgaccuccu1020
cgucggcgucaggcgccggacccgggaacucccggggcggauuagcgggucuccucacaa1080
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aaaacucugcugaucgagucauaacacaaacggcgggcaacacugccauaaacacgcaau1200
caucacuggguguguugugugccuacguugaagacccgaccaaaucugacccuccgucca1260
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acgcgagaaccucuaccucgguagacauaagugucccauacaucggggagacuccuacac1800
aauccucagagacacagaacuccuggacccuccucguuauggugcuugucccccuggacu1860
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ccacagcacccagagaaaauucgcuuauguuucucucgaccaucccugacgacacugucc2040
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cugccucagacgcuuaugugcccuacguugccguucccacccaguucgaugacaagccuc2220
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cugcggucgcuaagaaaguggucguccccauuacauucaugguucccaacagaccuucug5280
gacuuacacaguccgcccuucuuguggccggccggaccuuccuaauuaaugagcauacau5340
gguccaaccccuccuggaccaguuucacaauccguggugaggugcacacucgugaugagc5400
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caaaauuugaucccagacugaacaaggauguugaccuagaugagguaauuuggucuaaac6000
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aucggguuuucuccuuuuugggaaaagacaaugacauucugaccguuaaagaagcaaucc6120
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uuagcggccuucgacguacugaucucgucgauuuugugaacgguacgguagacgcagcac6240
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gugccuuugacucuucacacggcacuggcuccuucgaggcucucaucucucacuuuuuca6600
ccguugacaaugguuuuagcccugcgcugggaccguaucucagaucccuggcugucucgg6660
ugcacgcuuacggcgagcgucgcaucaagauuaccggaggccuucccucugguugugccg6720
cgaccagccuucugaacacagugcucaacaaugugaucaucaggacugcucuggcauuga6780
cuuacaaggaauuugaguaugacaugguugauaucaucgccuacggugacgaccuuuugg6840
uugguacggauuaugaucuggacuucaaugaaguggcgcggcgcgcugccaaacuggggu6900
auaagaugacuccugccaacaaggguuccgucuucccuccgacuuccucucucuccgaug6960
cuguuuuucuaaaacgcaaauucguccaaaacaaugacggcuuauauaaaccaguuaugg7020
auuuaaagaauuuggaagccaugcucuccuacuucaaaccaggaacacuacucgagaagc7080
ugcaaucuguuucuauguuggcucaacauucuggaaaagaagaauaugauagauugaugc7140
accccuucgcugacuacggugccguaccgagucacgaguaccugcaggcaagauggaggg7200
ccuuguucgauugaccuagauagcccaacgcgcuucggugccgccggcgauucugggaga7260
acucagucggaacagaaaagggaaaa7286
<210>6
<211>7270
<212>rna
<213>塞内卡病毒
<400>6
cugggcccucaugcccaguccuuccuuuccccuuccgggggguaaaccggcuguguuugc60
uagaggcacagaggagcaacauccaaccugcuuuuguggggaacggugcggcuccaauuc120
cugcgucgccaaagguguuagcgcacccaaacggcgcaucuaccaaugcuauuggugugg180
ucugcgaguucuagccuacucguuucuccccuauccacucacucacgcacaaaaagugug240
cuguaauuacaagauuuagcccucgcacgagaugugcgauaaccgcaagauugacucaag300
cgcggaaagcgcuguaaccacaugcuguuagucccuucauggcugcgagauggcuaucca360
ccucggaucacugaacuggagcucgacccuccuuaguaagggaaccgagaggccuucuug420
caacaagcuccgacacagaguccacgugauugcuaccaccaugaguacaugguucucccc480
ucucgacccaggacuucuuuuugaauauccacggcucgauccagaggguggggcaugauc540
ccccuagcauagcgagcuacagcgggaacuguagcuaggccuuagcgugccuuggauacu600
gccugauagggcgacggccuagucgugucgguucuauagguagcacauacaaauaugcag660
aacucucacuuuucuuucgauacagccucuggcaccuuugaagacguaaccggaacaaaa720
gucaagaucguugaauacccuagaucggugaacaaugguguuuacgauucguccacucau780
uuagagauacugaaccuacagggugaaauugaaauuuuaaagucuuucaaugaauaccaa840
auucgcgccgccaaacaacaacuuggacuggacaucguauacgaacuacaggguaauguu900
cagacaaccucaaagaaugauuuugauucccgcgguaauaaugguaacaugaccuucaau960
uacuacgcaaacacuuaccagaauucaguagacuucucgaccuccucgucggcgucaggc1020
gccggacccgggaacucccggggcggauuagcgggucuccucacaaauuucaguggaauc1080
uugaacccucuuggcuaccucaaagaucacaauaccgaagaaauggaaaacucugcugau1140
cgagucauaacgcaaacggcgggcaacacugccauaaacacgcaaucaucacugggugug1200
uugugugccuacguugaagacccgaccaaaucugacccuccguccagcagcacagaucaa1260
cccaccaccacuuuuacugccaucgacaggugguacacuggacgccucaauucuuggaca1320
aaagcuguaaaaaccuucucuuuucaggccgucccgcucccuggagccuuccugucuaga1380
cagggaggccucaacggaggggccuucacggcuacccuacauagacauuucuuaaugaag1440
ugcggguggcaggugcagguccaaugcaauuugacgcaauuccaccaaggugcucuucuu1500
guugccaugguccccgaaaccacccuugaugucaaaccugacggcaaggcaaagagcuua1560
caggagcugaaugaagaacaguggguggaaaugucugacgauuaccggaccgggaaaaac1620
augccuuuccagucucuuggcacauacuaucggcccccuaacuggacuuggggccccaau1680
uucaucaaccccuaucaaguaacaguuuucccacaccaaauucugaacgcgagaaccucu1740
accucgguagacauaagugucccauacaucggggagacuccuacacaauccucagagaca1800
cagaacuccuggacccuccucguuauggugcuugucccccuggacuacaaggagggagcc1860
acaacugacccagaaauuacauuuucuguaaggccuacaaguccuuacuucaaugggcua1920
cguaaccguuucacgaccgggacggacgaggaacaggggcccauucccacagcacccaga1980
gaaaauucgcuuauguuucucucaaccaucccugacgacacugucccugcuuacgggaau2040
gugcguaccccucccgucaauuaccucccuggugaaauaaccgaccucuuacaacuggcc2100
cguauacccacucucauggcguucgggcgggugucugaacccgagccugccucagacaca2160
uaugugcccuacguugccguuccugcccaguucgacgacaagccucucaucuccuucccg2220
aucacccuuucagauccugucuaccagaacacccugguaggcgccaucaguucgaauuuc2280
gccaacuaccggggguguauccaaaucacucugacauuuuguggacccaugauggcaaga2340
gggaaauuccuacucucguauucuccuccaaauggagcacaaccacagacccuuucugaa2400
gcuaugcagugcacguacucuauuugggauauaggcuugaacucuaguuggaccuuuguc2460
auccccuacaucucgcccagugauuaccgugaaacucgggcuauuaccaacucaguuuau2520
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ccacagagucccuguauucucuuuuucgccucugcuggugaggauuacacccuccgucuc2640
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cacacuaaugucaaguuccuguuugaucgaucucgauuacugaauguaauuaagguacug2820
gagaaggacgccgucuucccccguccuuuccccacagcaacaggugcacagcaggacgau2880
gguuacuuuugccuucuaacaccccgcccaacagucgcuucccgacccgccacucguuuc2940
ggccuguacgucaauccgucugacaguggcguucucgcuaacacuucacuggauuucaau3000
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cuggagccagaucuggaauucgcuguagggugguuccccucuggcagugaguaccaggcu3120
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gugcuucccgugcgcugggggggcgcuuccaagcuuucuuccgccacgcggggucuaccg3300
gcucaugcugacugggggaccauuuacgccuucaucccccguccuaaugagaagaaaagc3360
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agcaugcuccccuuccgcagcuacaagcagaagaugcugaugcaaucaggcgacgucgag3480
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cuagucacucuggccucucucuggaagaugguacacucuguucaacagaccuggagaaag3600
uaugugaagaaugacaauuuuuggcccaauuugcucagugagcuagugggggaaggcucc3660
aucgccuuggccgccacgcuaucuaaccaagcuucaguaaaagcucucuuaggccugcau3720
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aguuucuuuacuguagaaccgccuccuccaccagcugaaaaucugaugaccaagcccucc3840
gugaggucgaaauuccgaaagcuguuuaagaugcaaggacccauggacacagucaaagac3900
uggaaccaaauagccgccggccugaagaauuuccaauuuguucgugaccuggucaaagag3960
guggucgauuggcuccaggccuggauuaauaaagagaaagccagcccuguccuccaguac4020
cagcuggagaugaagaaacucggacccguggcuuuggcucaugaugccuucauggccggu4080
uccgggcccccucuuagugacgaccagauugaauaucuccagaaccucaaaucucuugcc4140
cuaacacuggggaagacuaauuuggcccaaagucucaccacuaugaucaaugccaagcaa4200
agcuccgcucaacgagucgaacccguuguggugguccuuagaggcaagccgggauguggc4260
aagagcuuggccuccacguugauugcccaggcuguguccaagcgucucuacggcucacaa4320
aguguguauucucuuccuccggauccagacuucuucgacggauacaaaggacaguuugua4380
accuugauggacgaucugggacaaaacccggaugggcaagauuucuccaccuuuugucag4440
auggugucgaccgcccaauuucuucccaacauggcggaccuuacagagaaggggcguccc4500
uucaccuccaaucuuaucauugcaacuacaaaccucccucacuucagcccugucaccauu4560
gcugauccuucugcagucucucgucguaucaacuacgaccugacucuagaaguaucugag4620
gccuauaagaagcacacacggcugaauuuugaccuggcuuucagacgcacagacgccccc4680
cccauuuauccuuuugcugcccaugugcccuucguggacguggcugugcgcuucaaaaau4740
ggucaucagagcuucaaucuccuagaguuggucgacucuauuugugcagacauucgagcc4800
aagcaacaaggugcccgaaauaugcagacucugguucuacagagccccaacgagaacgac4860
gacacccccgucgacgaggcguuggguagaguucucacccccgcugcggucgacgaggcg4920
cuugucgaccucgcuccagaugccgacccgguuggccguuuggcuauucucgccaagcua4980
ggucuugcccuagcugcggucaccccugguuugauaaucuuggcagugggacucuacagg5040
uacuucucuggcucugauacagaccaagaagaaacagaaggugaggagccugcuaaagcg5100
ccuaggagcgagaaugcuuaugacggcccgaagaaaaacucuaagcccccuggagcgcuc5160
ucccuuauggaaaugcaacagcccaacguggacaugggcuuugaggcugcggucgcuaag5220
aaaguggucguccccauuaccuucaugguucccaacagaccuucuggacuuacacagucc5280
gcucuucuuguggccggccggaccuuccuaauuaaugagcauacaugguccaaccccucc5340
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cauuuuacucaccaugguguucccacagaucuggugaugguacgucucggaccgggcaac5460
uccuucccuaacaaucuagacaaguuuggacuugaucagaugccggcacguaacucccgu5520
gugguuggcguuucggcuaguuacggcaacuucuucuucucugggaacuuccucggguuu5580
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accuacaagggguggugcgguucggcccugguuugugaggccggugguguccggcgcauc5700
auuggccugcauucugcuggugccgcugguaucggcgccgggacuuacaucucaaaauua5760
ggacugaucaaagcccucaaacaccucggugaaccucuggcuacaaugcaaggacugaug5820
acugagcuagagccuggagucaccgugcauguaccccggaaaucuaaauugagaaagacg5880
accgcacacgcgguguacaaaccggaguuugaaccugcuguguugucaaaauuugauccc5940
agacugaacaaggauguugaccuagaugagguaauuuggucuaaacacacugccaacguc6000
ccuuaucaaccuccuuuguucuacacauacaugucagaguacgcucaucggguuuucucc6060
uuuuugggaaaagacaaugacauucugaccgucaaagaagcaaucuugggaaucccugga6120
cuagacccuauggauccccacacagcuccgggucugcccuacgccauuagcggccuucga6180
cguacugaucucgucgauuuugcgaacggcacgguggacccgacacuggccguacaaauc6240
caaaaauucuuagacggugacuacucugaucaugucuuccaaacuuuucugaaagaugaa6300
aucagacccucagagaagguccgagcgggaaaaacccgcauugucgaugugcccucccug6360
gcgcacugcauugugggcagaauguugcuugggcgcuuugccgccaaguuucaaucccau6420
ccuggcuuucuccuuggcucugcuaucgggucugacccugaugucuucuggaccgucaua6480
ggggcucagcucgagggaagaaagaacacguaugacguggacuacagugccuuugacucu6540
ucacacggcacuggcuccuucgaggcucucaucucucacuuuuucaccguggacaauggu6600
uuuagcccugcgcugggaucguaucucagaucccuggcugucucggugcacgcuuacggc6660
gagcgucgcaucaagauuaccggaggccuccccucugguugugccgcgaccagccugcug6720
aacacagugcucaacaaugugaucaucaggacugcucuggcauugaccuacaaggaauuu6780
gaauaugacaugguugauaucaucgccuacggugacgaccuucugguugguacggauuac6840
gaucuggacuucaaugagguggcgcggcgcgcugccaaacugggguauaagaugacuccu6900
gccaacaaagguucugucuucccuccgacuuccucucucuccgacgcuguuuuucuaaaa6960
cgcaaauucguccaaaacaaugacggcuuauacaaaccaguuauggauuuaaagaauuug7020
gaagccaugcucuccuacuucaaaccaggaacacuacucgagaagcugcaaucuguuucu7080
auguuggcucaacauucuggaaaagaagaauaugauagauugaugcaccccuuugcugac7140
uacggugccguaccgagucacgaguaccugcaggcaagauggagggccuuguucgacuga7200
ccuagauagcccaacgcgcuccggugcugccggcgauucugggagaacucagucggaaca7260
gaaaagggaa7270
<210>7
<211>7270
<212>rna
<213>塞内卡病毒
<400>7
cugggcccucaugcccaguccuuccuuuccccuuccgggggguaaaccggcuguguuugc60
uagaggcacagaggagcaacauccaaccugcuuuuguggggaacggugcggcuccaauuc120
cugcgucgccaaagguguuagcgcacccaaacggcgcaucuaccaaugcuauuggugugg180
ucugcgaguucuagccuacucguuucuccccuauccacucacucacgcacaaaaagugug240
cuguaauuacaagauuuagcccucgcacgagaugugcgauaaccgcaagauugacucaag300
cgcggaaagcgcuguaaccacaugcuguuagucccuucauggcugcgagauggcuaucca360
ccucggaucacugaacuggagcucgacccuccuuaguaagggaaccgagaggccuucuug420
caacaagcuccgacacagaguccacgugauugcuaccaccaugaguacaugguucucccc480
ucucgacccaggacuucuuuuugaauauccacggcucgauccagaggguggggcaugauc540
ccccuagcauagcgagcuacagcgggaacuguagcuaggccuuagcgugccuuggauacu600
gccugauagggcgacggccuagucgugucgguucuauagguagcacauacaaauaugcag660
aacucucacuuuucuuucgauacagccucuggcaccuuugaagacguaaccggaacaaaa720
gucaagaucguugaauacccuagaucggugaacaaugguguuuacgauucguccacucau780
uuagagauacugaaccuacagggugaaauugaaauuuuaaagucuuucaaugaauaccaa840
auucgcgccgccaaacaacaacuuggacuggacaucguauacgaacuacaggguaauguu900
cagacaaccucaaagaaugauuuugauucccgcgguaauaaugguaacaugaccuucaau960
uacuacgcaaacacuuaccagaauucaguagacuucucgaccuccucgucggcgucaggc1020
gccggacccgggaacucccggggcggauuagcgggucuccucacaaauuucaguggaauc1080
uugaacccucuuggcuaccucaaagaucacaauaccgaagaaauggaaaacucugcugau1140
cgagucauaacgcaaacggcgggcaacacugccauaaacacgcaaucaucacugggugug1200
uugugugccuacguugaagacccgaccaaaucugacccuccguccagcagcacagaucaa1260
cccaccaccacuuuuacugccaucgacaggugguacacuggacgccucaauucuuggaca1320
aaagcuguaaaaaccuucucuuuucaggccgucccgcucccuggagccuuccugucuaga1380
cagggaggccucaacggaggggccuucacggcuacccuacauagacauuucuuaaugaag1440
ugcggguggcaggugcagguccaaugcaauuugacgcaauuccaccaaggugcucuucuu1500
guugccaugguccccgaaaccacccuugaugucaaaccugacggcaaggcaaagagcuua1560
caggagcugaaugaagaacaguggguggaaaugucugacgauuaccggaccgggaaaaac1620
augccuuuccagucucuuggcacauacuaucggcccccuaacuggacuuggggccccaau1680
uucaucaaccccuaucaaguaacaguuuucccacaccaaauucugaacgcgagaaccucu1740
accucgguagacauaagugucccauacaucggggagacuccuacacaauccucagagaca1800
cagaacuccuggacccuccucguuauggugcuugucccccuggacuacaaggagggagcc1860
acaacugacccagaaauuacauuuucuguaaggccuacaaguccuuacuucaaugggcua1920
cguaaccguuucacgaccgggacggacgaggaacaggggcccauucccacagcacccaga1980
gaaaauucgcuuauguuucucucaaccaucccugacgacacugucccugcuuacgggaau2040
gugcguaccccucccgucaauuaccucccuggugaaauaaccgaccucuuacaacuggcc2100
cguauacccacucucauggcguucgggcgggugucugaacccgagccugccucagacaca2160
uaugugcccuacguugccguuccugcccaguucgacgacaagccucucaucuccuucccg2220
aucacccuuucagauccugucuaccagaacacccugguaggcgccaucaguucgaauuuc2280
gccaacuaccggggguguauccaaaucacucugacauuuuguggacccaugauggcaaga2340
gggaaauuccuacucucguauucuccuccaaauggagcacaaccacagacccuuucugaa2400
gcuaugcagugcacguacucuauuugggauauaggcuugaacucuaguuggaccuuuguc2460
auccccuacaucucgcccagugauuaccgugaaacucgggcuauuaccaacucaguuuau2520
ucugcugaugguugguuuagccugcacaagcugacuaaaauuacucuaccaccugacugc2580
ccacagagucccuguauucucuuuuucgccucugcuggugaggauuacacccuccgucuc2640
ccuguugauuguaauccuuccuacguguuccacuccaccgacaacgccgagacugggguu2700
auugaggcggguaacacugacaccgacuucucuggugagcuggcggcuccuggcucuaac2760
cacacuaaugucaaguuccuguuugaucgaucucgauuacugaauguaauuaagguacug2820
gagaaggacgccgucuucccccguccuuuccccacagcaacaggugcacagcaggacgau2880
gguuacuuuugccuucuaacaccccgcccaacagucgcuucccgacccgccacucguuuc2940
ggccuguacgucaauccgucugacaguggcguucucgcuaacacuucacuggauuucaau3000
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cuggagccagaucuggaauucgcuguagggugguuccccucuggcagugaguaccaggcu3120
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gcuuucaccagcaaggguggaaagguaucuuucgugcuuccuuggaacucugucucaucc3240
gugcuucccgugcgcugggggggcgcuuccaagcuuucuuccgccacgcggggucuaccg3300
gcucaugcugacugggggaccauuuacgccuucaucccccguccuaaugagaagaaaagc3360
accgcugcaaagcacguggcgguguacguucgguacaagaacgcgcgugccuggugcccc3420
agcaugcuccccuuccgcagcuacaagcagaagaugcugaugcaaucaggcgacgucgag3480
accaacccuggcccugcuucugacaacccaaucuuggaguuucuugaagcugaaaacgau3540
cuagucacucuggccucucucuggaagaugguacacucuguucaacagaccuggagaaag3600
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aucgccuuggccgccacgcuaucuaaccaagcuucaguaaaagcucucuuaggccugcau3720
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aguuucuuuacuguagaaccgccuccuccaccagcugaaaaucugaugaccaagcccucc3840
gugaggucgaaauuccgaaagcuguuuaagaugcaaggacccauggacacagucaaagac3900
uggaaccaaauagccgccggccugaagaauuuccaauuuguucgugaccuggucaaagag3960
guggucgauuggcuccaggccuggauuaauaaagagaaagccagcccuguccuccaguac4020
cagcuggagaugaagaaacucggacccguggcuuuggcucaugaugccuucauggccggu4080
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cuaacacuggggaagacuaauuuggcccaaagucucaccacuaugaucaaugccaagcaa4200
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aagagcuuggccuccacguugauugcccaggcuguguccaagcgucucuacggcucacaa4320
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auggugucgaccgcccaauuucuucccaacauggcggaccuuacagagaaggggcguccc4500
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gcugauccuucugcagucucucgucguaucaacuacgaccugacucuagaaguaucugag4620
gccuauaagaagcacacacggcugaauuuugaccuggcuuucagacgcacagacgccccc4680
cccauuuauccuuuugcugcccaugugcccuucguggacguggcugugcgcuucaaaaau4740
ggucaucagagcuucaaucuccuagaguuggucgacucuauuugugcagacauucgagcc4800
aagcaacaaggugcccgaaauaugcagacucugguucuacagagccccaacgagaacgac4860
gacacccccgucgacgaggcguuggguagaguucucacccccgcugcggucgacgaggcg4920
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ggucuugcccuagcugcggucaccccugguuugauaaucuuggcagugggacucuacagg5040
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ccuaggagcgagaaugcuuaugacggcccgaagaaaaacucuaagcccccuggagcgcuc5160
ucccuuauggaaaugcaacagcccaacguggacaugggcuuugaggcugcggucgcuaag5220
aaaguggucguccccauuaccuucaugguucccaacagaccuucuggacuuacacagucc5280
gcucuucuuguggccggccggaccuuccuaauuaaugagcauacaugguccaaccccucc5340
uggaccagcuucacaauccguggugaggugcacacccgugaugagccuuuccaaacgguu5400
cauuuuacucaccaugguguucccacagaucuggugaugguacgucucggaccgggcaac5460
uccuucccuaacaaucuagacaaguuuggacuugaucagaugccggcacguaacucccgu5520
gugguuggcguuucggcuaguuacggcaacuucuucuucucugggaacuuccucggguuu5580
guugacuccaucaccucugaccaaggaacuuaugcgagacuuuuuagguacagggugacg5640
accuacaagggguggugcgguucggcccugguuugugaggccggugguguccggcgcauc5700
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ggacugaucaaagcccucaaacaccucggugaaccucuggcuacaaugcaaggacugaug5820
acugagcuagagccuggagucaccgugcauguaccccggaaaucuaaauugagaaagacg5880
accgcacacgcgguguacaaaccggaguuugaaccugcuguguugucaaaauuugauccc5940
agacugaacaaggauguugaccuagaugagguaauuuggucuaaacacacugccaacguc6000
ccuuaucaaccuccuuuguucuacacauacaugucagaguacgcucaucggguuuucucc6060
uuuuugggaaaagacaaugacauucugaccgucaaagaagcaaucuugggaaucccugga6120
cuagacccuauggauccccacacagcuccgggucugcccuacgccauuagcggccuucga6180
cguacugaucucgucgauuuugcgaacggcacgguggacccgacacuggccguacaaauc6240
caaaaauucuuagacggugacuacucugaucaugucuuccaaacuuuucugaaagaugaa6300
aucagacccucagagaagguccgagcgggaaaaacccgcauugucgaugugcccucccug6360
gcgcacugcauugugggcagaauguugcuugggcgcuuugccgccaaguuucaaucccau6420
ccuggcuuucuccuuggcucugcuaucgggucugacccugaugucuucuggaccgucaua6480
ggggcucagcucgagggaagaaagaacacguaugacguggacuacagugccuuugacucu6540
ucacacggcacuggcuccuucgaggcucucaucucucacuuuuucaccguggacaauggu6600
uuuagcccugcgcugggaucguaucucagaucccuggcugucucggugcacgcuuacggc6660
gagcgucgcaucaagauuaccggaggccuccccucugguugugccgcgaccagccugcug6720
aacacagugcucaacaaugugaucaucaggacugcucuggcauugaccuacaaggaauuu6780
gaauaugacaugguugauaucaucgccuacggugacgaccuucugguugguacggauuac6840
gaucuggacuucaaugagguggcgcggcgcgcugccaaacugggguauaagaugacuccu6900
gccaacaaagguucugucuucccuccgacuuccucucucuccgacgcuguuuuucuaaaa6960
cgcaaauucguccaaaacaaugacggcuuauacaaaccaguuauggauuuaaagaauuug7020
gaagccaugcucuccuacuucaaaccaggaacacuacucgagaagcugcaaucuguuucu7080
auguuggcucaacauucuggaaaagaagaauaugauagauugaugcaccccuuugcugac7140
uacggugccguaccgagucacgaguaccugcaggcaagauggagggccuuguucgacuga7200
ccuagauagcccaacgcgcuccggugcugccggcgauucugggagaacucagucggaaca7260
gaaaagggaa7270
<210>8
<211>7270
<212>rna
<213>塞内卡病毒
<400>8
cugggcccucaugcccaguccuuccuuuccccuuccgggggguaaaccggcuguguuugc60
uagaggcacagaggagcaacauccaaccugcuuuuguggggaacggugcggcuccaauuc120
cugcgucgccaaagguguuagcgcacccaaacggcgcaucuaccaaugcuauuggugugg180
ucugcgaguucuagccuacucguuucucccuuauccacucacucacgcacaaaaagugug240
cuguaacuacaagauuuagcccucgcacgagaugugcgauaaccgcaagauugacucaag300
cgcggaaagcgcuguaaccacaugcuguuagucccuucauggcugcgagauggcuaucca360
ccucggaucacugaacuggagcucgacccuccuuaguaagggaaccgagaggccuucuug420
caacaagcuccgacacagaguccacgugauugcuaccaccaugaguacaugguucucccc480
ucucgacccaggacuucuuuuugaauauccacggcucgauccagaggguggggcaugauc540
ccccuagcauagcgagcuacagcgggaacuguagcuaggccuuagcgugccuuggauacu600
gccugauagggcgacggccuagucgugucgguucuauagguagcacauacaaauaugcag660
aacucucauuuuucuuucgauacagccucuggcaccuuugaagacguaaccggaacaaaa720
gucaagaucguugaacacccuagaucggugaacaaugguguuuacgauucguccacucau780
uuagagauacugaaccuacagggugaaauugaaauuuuaaagucuuucaaugaauaccaa840
auucgcgccgccaaacaacaacuuggacuggacaucguauacgaacuacaggguaauguu900
cagacaaccucaaagaaugauuuugauucccgcgguaauaaugguaacaugaccuucaau960
uacuacgcaaacacuuaccagaauucaguagacuucucgaccuccucgucggcgucaggc1020
gccggacccgggaacucccggggcggauuagcgggucuccucacaaauuucaguggaauc1080
uugaacccucuuggcuaccucaaagaucacaauaccgaagaaauggaaaacucugcugau1140
cgagucauaacgcaaacggcgggcaacacugccauaaacacgcaaucaucacugggugug1200
uugugugccuacguugaagacccgaccaaaucugacccuccguccagcagcacagaucaa1260
cccaccaccacuuuuacugccaucgacaggugguacacuggacgccucaauucuuggaca1320
aaagcuguaaaaaccuucucuuuucaggccgucccgcucccuggagccuuccugucuaga1380
cagggaggccucaacggaggggccuucacggcuacccuacauagacauuucuuaaugaag1440
ugcggguggcaggugcagguccaaugcaauuugacgcaauuccaccaaggugcucuucuu1500
guugccaugguccccgaaaccacccuugaugucaaaccugacggcaaggcaaagagcuua1560
caggagcugaaugaagaacaguggguggaaaugucugacgauuaccggaccgggaaaaac1620
augccuuuccagucucuuggcacauacuaucggcccccuaacuggacuuggggccccaau1680
uucaucaaccccuaucaaguaacaguuuucccacaccaaauucugaacgcgagaaccucu1740
accucgguagacauaagugucccauacaucggggagacuccuacacaauccucagagaca1800
cagaacuccuggacccuccucguuauggugcuugucccccuggacuacaaggagggagcc1860
acaacugacccagaaauuacauuuucuguaaggccuacaaguccuuauuucaaugggcua1920
cguaaccguuucacgaccgggacggacgaggaacaggggcccauucccacagcacccaga1980
gaaaauucgcuuauguuucucucaaccaucccugacgacacugucccugcuuacgggaau2040
gugcguaccccucccgucaauuaccucccuggugaaauaaccgaccucuuacaacuggcc2100
cguauacccacucucauggcguuugggcgggugucugaacccgagccugccucagacaca2160
uaugugcccuacguugccguuccugcccaguucgacgacaagccucucaucuccuucccg2220
aucacccuuucagauccugucuaccagaacacccugguaggcgccaucaguucgaauuuc2280
gccaacuaccggggguguauccaaaucacucugacauuuuguggacccaugauggcaaga2340
gggaaauuccuacucucguauucuccaccaaauggagcacaaccacagacccuuucugaa2400
gcuaugcagugcacguacucuauuugggauauaggcuugaacucuaguuggaccuuuguc2460
auccccuacaucucgcccagugauuaccgugaaacucgggcuauuaccaacucaguuuau2520
ucugcugaugguugguuuagccugcacaagcuaacuaaaauuacucuaccaccugacugc2580
ccacagagucccuguauucucuuuuucgccucugcuggugaggauuacacccuccgucuc2640
ccuguugauuguaauccuuccuacguguuccacuccaccgacaacgccgagacugggguu2700
auugaggcggguaacacugacaccgauuucucuggugagcuggcggcuccuggcucuaac2760
cacacuaaugucaaguuccuguuugaucgaucucgauuacugaauguaauuaagguacug2820
gagaaggacgccgucuucccccguccuuuccccacagcaacaggugcacagcaggacgau2880
gguuacuuuugccuucuaacaccccgcccaacagucgcuucccgacccgccacccguuuc2940
ggccuguacgucaauccgucugacaguggcguucucgcuaacacuucacuggauuucaau3000
uuuuacagcuuggccuguuucacuuacuuuagaucagaccuugaagucacgguggucuca3060
cuggagccagaucuggaauucgcuguagggugguuccccucuggcagugaguaccaggcu3120
uccagcuuugucuacgaccaacugcauguacccuaccacuuuacugggcgcacuccccgc3180
gcuuucaccagcaaggguggaaagguaucuuucgugcuuccuuggaacucugucucaucc3240
gugcuucccgugcgcugggggggcgcuuccaagcuuucuuccgccacgcggggucuaccg3300
gcucaugcugacugggggaccauuuacgccuucaucccccguccuaacgagaagaaaagc3360
accgcuguaaagcacguggcgguguacguucgguacaagaacgcgcgugccuggugcccc3420
agcaugcuccccuuccgcagcuacaagcagaagaugcugaugcaaucaggcgacgucgag3480
accaacccuggcccugcuucugacaacccaaucuuggaguuucuugaagcugaaaacgau3540
cuagucacucuggccucucucuggaagaugguacacucuguucaacagaccuggagaaag3600
uaugugaaaaacgacaauuuuuggcccaauuugcucagugagcuagugggggaaggcucc3660
aucgccuuggccgccacgcuaucuaaccaagcuucaguaaaagcucucuuaggccugcau3720
uuucucucccgagggcucaauuauacagauuuuuacucuuuacugauagagaaaugcucu3780
aguuucuuuacuguagaaccgccuccuccaccagcugaaaaucugaugaccaagcccucc3840
gugaggucgaaauuccgaaagcuguuuaagaugcaaggacccauggacacagucaaagac3900
uggaaccaaauagccgccggccugaagaauuuccaauuuguucgugaccuggucaaagag3960
guggucgauuggcuccaggccuggauuaauaaagagaaagccagcccuguccuccaguac4020
cagcuggagaugaagaaacucggacccguggcuuuggcucaugaugccuucauggccggu4080
uccgggcccccucuuagugacgaccagauugaauaucuccagaaccucaaaucucuugcc4140
cuaacacuggggaagacuaauuuggcccaaagucucaccacuaugaucaaugccaagcaa4200
agcuccgcucaacgagucgaacccguuguggugguccuuagaggcaagccgggauguggc4260
aagagcuuggccuccacguugauugcccaggccguguccaagcgucucuacggcucacaa4320
aguguguauucucuuccuccggauccagacuucuucgacggauacaaaggacaguuugua4380
accuugauggacgaucugggacaaaacccggaugggcaagauuucuccaccuuuugucag4440
auggugucgaccgcccaauuucuucccaacauggcggaccuugcagaaaaggggcguccc4500
uucaccuccaaucuuaucauugcaacuacaaaccucccucacuucagcccugucaccauu4560
gcugauccuuccgcagucucucgucguaucaacuacgaccugacucuagaaguaucugag4620
gccuauaagaagcacacacggcugaauuuugaccuggcuuucagacgcacagacgccccc4680
cccauuuauccuuuugcugcccaugugcccuucguggacguggcugugcgcuucaaaaau4740
ggucaucagagcuucaaucuccuagaguuggucgacucuauuugugcagacauucgagcc4800
aagcaacaaggugcucgaaauaugcagacucugguucuacagagccccaacgagaacgac4860
gacacccccgucgacgaggcguuggguagaguucucacccccgcugcggucgacgaggcg4920
cuugucgaccucgcuccagaugccgacccgguuggccguuuggcuauucucgccaagcua4980
ggucuugcccuagcugcggucaccccugguuugauaaucuuggcagugggacucuacagg5040
uacuucucuggcucugauacagaccaagaagaaacagaaagugaggagccugcuaaagcg5100
ccuaggagcgagaaugcuuaugacggcccgaagaaaaacuccaagcccccuggagcgcuc5160
ucccuuauggaaaugcaacagcccaacguggacaugggcuuugaggcugcggucgcuaag5220
aaaguggucguccccauuaccuucaugguucccaacagaccuucuggacuuacacagucc5280
gcucuucuuguggccggccggaccuuccuaauuaaugagcauacaugguccaaccccucc5340
uggaccagcuucacaauccguggugaggugcacgcucgugaugagccuuuccaaacgguu5400
cauuuuacucaccaugguguucccacagaucuagugaugguacgucucggaccgggcaac5460
ucuuucccuaacaaucuagacaaguuuggacuugaucagaugccggcacguaacucccgu5520
gugguuggcguuucggcuaguuacggcaacuucuucuucucugggaacuuccucggguuu5580
guugacuccaucaccucugaccaaggaacuuaugcgagacuuuuuagguacagggugacg5640
acuuacaagggauggugcgguucggcccugguuugugaggccggugguguccggcgcauc5700
auuggccugcauucugcuggugccgcugguaucggcgccgggacuuacaucucaaaauua5760
ggacugaucaaagcccucaaacaccucggugaaccucuggcuacaaugcaagggcugaug5820
acugagcuagagccuggagucaccgugcauguaccccggaaaucuaaauugagaaagacg5880
accgcacacgcgguguacaaaccggaguuugaaccugcuguguugucaaaauuugauccc5940
agacugaacaaggauguugaccuagaugaggcaauuuggucuaaacacacugccaacguc6000
ccuuaucaaccuccuuuguucuacacauacaugucagaguacgcucaucggguuuucucc6060
uuuuugggaaaagacaaugacauucugaccgucaaagaagcaauuuugggaaucccugga6120
cuagacccuauggauccccacacagcuccgggucugcccuacgccauuagcggccuucga6180
cguacugaucucgucgauuuugcgaacggcacgguagacccgauacuggccaugcaaauc6240
caaaaauucuuagacggugacuacucugaucaugucuuccaaacuuuucugaaagaugaa6300
aucagacccucagagaagguccgagcgggaaaaacccgcauugucgaugugcccucccug6360
gcgcacugcauugugggcagaauguugcuugggcgcuuugccgccaaguuucaaucccau6420
ccuggcuuucuccuuggcucugcuauugggucugacccugaugucuucuggaccgucaua6480
ggggcucagcucgagggaagaaagaacacguaugacguggacuacagugccuuugacucu6540
ucacacggcacuggcuccuucgaggcucucaucucucacuuuuucaccguggacaauggu6600
uuuagcccugcgcugggaccguaucucagaucccuggcugucucggugcacgcuuacggc6660
gagcgucgcaucaagauuaccggaggccuccccucugguugugccgcgaccagccugcug6720
aacacagugcucaacaaugugaucaucaggacugcucuggcauugaccuacaaggaauuu6780
gaauaugacaugguugauaucaucgccuacggugacgaccuucugguugguacggauuac6840
gaucuggacuucaaugagguggcgcggcgcgcugccaaacugggguauaagaugacuccu6900
gccaacaaagguucugucuucccuccgacuuccucucucuccgacgcuguuuuucuaaaa6960
cgcaaauucguccaaaacaaugacggcuuauacaaaccaguuauggauuuaaagaauuug7020
gaagccaugcucuccuacuucaaaccaggaacacuacucgagaagcugcaaucuguuucu7080
auguuggcucaacauucuggaaaagaagaauaugauagauugaugcaccccuuugcugac7140
uacggugccguaccgagucacgaguaccugcaggcaagauggagggccuuguucgacuga7200
ccuagauagcccaacgcgcuccggugcugccggcgauucugggagaacucagucggaaca7260
gaaaagggaa7270
<210>9
<211>7310
<212>rna
<213>塞内卡病毒
<400>9
uuugaaauggggggcugggcccugaugcccaguccuuccuuuccccuuccgggggguuaa60
ccggcuguguuugcuagaggcacagaggggcaacauccaaccugcuuuugcggggaacgg120
ugcggcuccgauuccugcgucgccaaagguguuagcgcacccaaacggcgcaccuaccaa180
uguuauugguguggucugcgaguucuagccuacucguuucucccccgaccauucacucac240
ccacgaaaaguguguuguaaccauaagauuuaacccccgcacgggaugugcgauaaccgu300
aagacuggcucaagcgcggaaagcgcuguaaccacaugcuguuagucccuuuauggcugc360
aagauggcuacccaccucggaucacugaacuggagcucgacccuccuuaguaagggaacc420
gagaggccuucgugcaacaagcuccgacacagaguccacgugacugcuaccaccaugagu480
acaugguucuccccucucgacccaggacuucuuuuugaauauccacggcucgauccagag540
gguggggcaugaccccuagcauagcgagcuacagcgggaacuguagcuaggccuuagcgu600
gccuuggauacugccugauagggcgacggccuagucgugucgguucuauagguagcacau660
acaaauaugcagaacucucauuuuucuuucgauacagccucuggcaccuuugaagaugua720
accggaacaaaagucaagaucguugaauaccccagaucggugaacaaugguguuuacgau780
ucgucuacucauuuggagauacugaaccuacagggugaaauugaaauuuuaaggucuuuc840
aaugaauaccaaauucgcgccgccaaacaacaacucggacuggacaucguguacgaacua900
caggguaauguucagacaacgucaaagaaugauuuugauucccguggcaauaaugguaac960
augaccuucaauuacuacgcaaacacuuaucagaauucaguagacuucucgaccuccucg1020
ucggcgucaggcgccggacccgggaacucccggggcggauuagcgggucuccucacaaau1080
uucaguggaaucuugaacccucuuggcuaccucaaagaucacaacaccgaagaaauggaa1140
aacucugcugaucgagucacaacgcaaacggcgggcaacacugccauaaacacgcaauca1200
ucauuggguguguugugugccuacguugaagacccgaccaaaucugauccuccguccagc1260
agcacagaucaacccaccaccacuuucacugccaucgacaggugguacacuggacgucuc1320
aauucuuggacaaaagcuguaaaaaccuucucuuuucaggccgucccgcuucccggggcc1380
uuucugucuaggcagggaggccucaacggaggggccuucacagcuacccuacauagacac1440
uuuuugaugaagugcggguggcaggugcagguccaauguaauuugacacaauuccaccaa1500
ggcgcucuucuuguugccaugguuccugaaaccacccuugaugucaagcccgacgguaag1560
gcaaagagcuuacaggagcugaaugaagaacaguggguggaaaugucugacgauuaccgg1620
accgggaaaaacaugccuuuucagucucuuggcacauacuaucggcccccuaacuggacu1680
uggggucccaauuucaucaaccccuaucaaguaacgguuuucccacaccaaauucugaac1740
gcgagaaccucuaccucgguagacauaaacgucccauacaucggggagacccccacgcaa1800
uccucagagacacagaacuccuggacccuccucguuauggugcucguuccccuagacuau1860
aaggaaggagccacaacugacccagaaauuacauuuucuguaaggccuacaagucccuac1920
uucaaugggcuucgcaaccgcuacacggccgggacggacgaagaacaggggcccauuccu1980
acggcacccagagaaaauucgcuuauguuucucucaacccucccugacgacacugucccu2040
gcuuacgggaaugugcguaccccuccugucaauuaccucccuggugaaauaaccgaccuu2100
uugcaacuggcccgcauacccacucucauggcauuugagcgggugccugaacccgugccu2160
gccucagacacauaugugcccuacguugccguucccacccaguucgaugacaggccucuc2220
aucuccuucccgaucacccuuucagaucccgucuaucagaacacccugguuggcgccauc2280
aguucaaauuucgccaauuaccguggguguauccaaaucacucugacauuuuguggaccc2340
augauggcgagagggaaauuccugcucucguauucucccccaaauggaacgcaaccacag2400
acucuuuccgaagcuaugcagugcacauacucuauuugggacauaggcuugaacucuagu2460
uggaccuucgucguccccuacaucucgcccagugacuaccgugaaacucgagccauuacc2520
aacucgguuuacuccgcugaugguugguuuagccugcacaaguugaccaaaauuacucua2580
ccaccugacuguccgcaaagucccugcauucucuuuuucgcuucugcuggugaggauuac2640
acucuccgucuccccguugauuguaauccuuccuauguguuccacuccaccgacaacgcc2700
gagaccgggguuauugaggcggguaacacugacaccgauuucucuggugaacuggcggcu2760
ccuggcucuaaccacacuaaugucaaguuccuguuugaucgaucucgauuauugaaugua2820
aucaagguacuggagaaggacgccguuuucccccgcccuuucccuacacaagaaggugcg2880
cagcaggaugaugguuacuuuugucuucugaccccccgcccaacagucgcuucccgaccc2940
gccacucguuucggccuguacgccaauccguccggcagugguguucuugcuaacacuuca3000
cuggacuucaauuuuuauagcuuggccuguuucacuuacuuuagaucggaccuugagguu3060
acgguggucucacuagagccggaucuggaauuugcuguagggugguuuccuucuggcagu3120
gaauaccaggcuuccagcuuugucuacgaccagcugcaugugcccuuccacuuuacuggg3180
cgcacuccccgcgcuuucgcuagcaagggugggaagguaucuuucgugcucccuuggaac3240
ucugucucgucugugcuccccgugcgcugggggggggcuuccaagcucucuucugcuacg3300
cggggucuaccggcgcaugcugauugggggacuauuuacgccuuugucccccguccuaau3360
gagaagaaaagcaccgcuguaaaacacguggccguguacauucgguacaagaacgcacgu3420
gccuggugccccagcaugcuucccuuucgcagcuacaagcagaagaugcugaugcaaucu3480
ggcgauaucgagaccaauccugguccugcuucugacaacccaauuuuggaguuucuugaa3540
gcagaaaaugaucuagucacucuggccucucucuggaagauggugcacucuguucaacag3600
accuggagaaaguaugugaagaacgaugauuuuuggcccaauuuacucagcgagcuagug3660
ggggaaggcucugucgccuuggccgccacgcuauccaaccaagcuucaguaaaggcucuu3720
uugggccugcacuuucucucucgggggcucaauuacacugacuuuuacucuuuacugaua3780
gagaaaugcucuaguuucuuuaccguagaaccaccuccuccaccagcugaaaaccugaug3840
accaagcccucagugaagucgaaauuccgaaaacuguuuaagaugcaaggacccauggac3900
aaagucaaagacuggaaccaaauagcugccggcuugaagaauuuucaauuuguucgugac3960
cuagucaaagagguggucgauuggcugcaggccuggaucaacaaagagaaagccagcccu4020
guccuccaguaccaguuggagaugaagaagcucgggccuguggccuuggcucaugacgcu4080
uucauggcugguuccgggcccccucuuagcgacgaccagauugaauaccuccagaaccuc4140
aaaucucuugcccuaacacuggggaagacuaauuuggcccaaagucucaccacuaugauc4200
aaugccaaacaaaguucagcccaacgaguugaacccguuguggugguccuuagaggcaag4260
ccgggaugcggcaagagcuuggccucuacguugauugcccaggcuguguccaagcgccuc4320
uauggcucccaaaguguauauucucuucccccagauccagauuucuucgauggauacaaa4380
ggacaguucgugaccuugauggaugauuugggacaaaacccggauggacaagauuucucc4440
accuuuugucagauggugucgaccgcccaauuucuccccaacauggcggaccuugcagag4500
aaagggcgucccuuuaccuccaaucucaucauugcaacuacaaaucucccccacuucagu4560
ccugucaccauugcugauccuucugcagucucucgccguaucaacuacgaucugacucua4620
gaaguaucugaggccuacaagaaacacacacggcugaauuuugacuuggcuuucaggcgc4680
acagacgccccccccauuuauccuuuugcugcccaugugcccuuuguggacguagcugug4740
cgcuucaaaaauggucaccagaauuuuaaucuccuagaguuggucgauuccauuuguaca4800
gacauucgagccaagcaacaaggugcccgaaacaugcagacucugguucuacagagcccc4860
aacgagaaugaugacacccccgucgacgaggcguuggguagaguucucucccccgcugcg4920
gucgaugaggcgcuugucgaccucacuccagaggccgacccgguuggccguuuggcuauu4980
cuugccaagcuaggucuugcccuagcugcggucaccccuggucugauaaucuuggcagug5040
ggacucuacagguacuucucuggcucugaugcagaccaagaagaaacagaaagugaggga5100
ucugucaaggcacccaggagcgaaaaugcuuaugacggcccgaagaaaaacucuaagccc5160
ccuggagcacucucucucauggaaaugcaacagcccaacguggacaugggcuuugaggcu5220
gcggucgcuaagaaaguggucguccccauuaccuucaugguucccaacagaccuucuggg5280
cuuacacaguccgcucuucuggugaccggccggaccuuccuaaucaaugaacauacaugg5340
uccaaucccuccuggaccagcuucacaauccgcggugagguacacacucgugaugagccc5400
uuccaaacgguucauuucacucaccacgguauucccacagaucugaugaugguacgucuc5460
ggaccgggcaauucuuucccuaacaaucuagacaaguuuggacuugaccagaugccggca5520
cgcaacucccgugugguuggcguuucguccaguuacggaaacuucuucuucucuggaaau5580
uuccucggauuuguugauuccaucaccucugaacaaggaacuuacgcaagacucuuuagg5640
uacagggugacgaccuacaaaggauggugcggcucggcccuggucugugaggccgguggc5700
guccgacgcaucauuggccugcauucugcuggcgccgccgguaucggcgccgggaccuau5760
aucucaaaauuaggacuaaucaaagcccugaaacaccucggugaaccuuuggccacaaug5820
caaggacugaugacugaauuagagccuggaaucaccguacauguaccccggaaauccaaa5880
uugagaaagacgaccgcacacgcgguguacaaaccggaguuugagccugcuguguuguca5940
aaauuugaucccagacugaacaaggauguugacuuggaugaaguaauuuggucuaaacac6000
acugccaaugucccuuaccaaccuccuuuguucuacacauacaugucagaguacgcucau6060
cgagucuucuccuucuuggggaaagacaaugacauucugaccgucaaagaagcaauucug6120
ggcauccccggacuagaccccauggauccccacacagcuccgggucugccuuacgccauc6180
aacggccuucgacguacugaucucgucgauuuugugaacgguacaguagaugcggcgcug6240
gcuguacaaauccagaaauucuuagacggugacuacucugaccaugucuuccaaacuuuu6300
cugaaagaugagaucagacccucagagaaaguccgagcgggaaaaacccgcauuguugau6360
gugcccucccuggcgcauugcauugugggcagaauguugcuugggcgcuuugcugccaag6420
uuucaaucccauccuggcuuucuccucggcucugcuaucgggucugacccugauguuuuc6480
uggaccgucauaggggcucaacucgaggggagaaagaacacguaugacguggacuacagu6540
gccuuugacucuucacacggcacuggcuccuucgaggcucucaucucucacuuuuucacc6600
guggacaaugguuuuagcccugcgcugggaccguaucucagaucccuggcugucucggug6660
cacgcuuacggcgagcgucgcaucaagauuaccgguggccuccccuccgguugugccgcg6720
accagccugcugaacacagugcucaacaaugugaucaucaggacugcucuggcauugacu6780
uacaaggaauuugaauaugacaugguugauaucaucgccuacggugacgaccuucugguu6840
ggcacggauuacgaucuggacuucaaugagguggcacgacgcgcugccaaguugggguau6900
aagaugacuccugccaacaaggguucugucuucccuccgacuuccucucuuuccgaugcu6960
guuuuucuaaagcgcaaauucguccaaaacaacgacggcuuauacaaaccaguuauggau7020
uuaaagaauuuggaagccaugcucuccuacuucaaaccaggaacacuacucgagaagcug7080
caaucuguuucuauguuggcucaacauucuggaaaagaagaauaugauagauugaugcac7140
cccuucgcugacuacggugccguaccgagucacgaguaccugcaggcaagauggagggcc7200
uuguucgacugacccagauagcccaaggcgcuucggugcugccggcgauucugggagaac7260
ucagucggaacagaaaagggaaaaaaaaaaaaaaaaaaaaaaaaaaaaaa7310
<210>10
<211>7356
<212>rna
<213>塞内卡病毒
<400>10
aggggggggcugggcccucaugcccaguccuuccuuuccccuuccgggggguaaaccggc60
uguguuugcuagaggcacagaggagcaacacccaaccugcuuuuguggggaacggugcgg120
cuccgauuccugcgucgccaaagguguuagcgcacccaaacggcgcaucuaccaaugcua180
uugguguggucugcgaguucuagccuacucguuucuccccuauccauucacucacgcaca240
aaaaguguguuguaacuacaagauuuagcccucgcacgagaugugcgauaaccgcaagac300
ugacucaagcgcggaaagcgcuguaaccacaugcuguuagucccuuuauggcugcgagau360
ggcuauccaccucggaucacugaacuggagcucgacccuccuuaguaagggaaccgagag420
gccuucuugcaacaagcuccgacacagaguccacgugauugcuaccaccaugaguacaug480
guucuccccucucgacccaggacuucuuuuugaauauccacggcucgauccagagggugg540
ggcaugaucccccuagcauagcgagcuacagcgggaacuguagcuaggccuuagcgugcc600
uuggauacugccugauagggcgacggccuagucgugucgguucuauagguagcacauaua660
aauaugcagaacucucauuuuucuuucgauacagccucuggcaccuuugaagacguaacc720
ggaacaaaagucaagaucguugaauaccccagaucggugaacaaugguguuuacgauucg780
uccacucauuuagagauacugaaccuacagggugaaauugaaauuuuaaggucuuucaau840
gaauaccaaauucgcgccgccaaacaacaacuuggacuggacauuguauacgaacuacag900
gguaauguucagacaaccucaaagaaugauuuugauucccgcggcaauaacgguaacaug960
accuucaauuacuacgcaaacacuuaucagaauucaguagacuucucgaccuccucgucg1020
gcgucaggcgccgggcccgggaacucccggggcggauuagcgggucuccucacaaauuuc1080
aguggaaucuugaacccucuuggcuaccucaaagaucacaauaccgaagaaauggaaaac1140
ucugcugaucgagucauaacgcaaacggcgggcaacacugccauaaacacgcaaucauca1200
cuggguguguugugugccuauguugaagacccgaccaaaucugacccuccguccagcagc1260
acagaucaacccaccaccacuuuuacugccaucgacaggugguacacuggacgccucaau1320
ucuuggacaaaagcuguaaaaaccuucucuuuucaggccgucccgcucccuggagccuuc1380
cugucuagacagggaggccucaacggaggggccuucacggcuacccuacacagacauuuc1440
uuaaugaagugcggguggcaggugcagguccaaugcaauuugacacaauuccaccaaggu1500
gcucuucuuguugccaugguccccgaaaccacccuygaugucaagcccgacggcaaggca1560
aagagccuacaggagcugaaugaagagcaguggguagaaaugucugacgauuaccggacc1620
gggaaaaacaugccuuuucagucucuuggcacauacuaucggcccccuaacuggacuugg1680
ggcccuaauuucaucaaccccuaucaaguaacaguuuucccacaccaaauucugaacgcg1740
agaaccucuaccucgguagacauaagugucccauacaucggggagacuccuacacaaucc1800
ucagagacacagaacuccuggacccuccucguuauggugcuugucccccuggacuacaag1860
gagggagccacaacugacccagaaauuacauuuucuguaaggccuacaaguccuuacuuc1920
aaugggcuucguaaccguuacacgaccgggacggacgaggaacaggggcccauucccaca1980
gcacccagagaaaauucgcuuauguuucucucaaccaucccugacgacacugucccugcu2040
uacgggaaugugcguaccccucccgucaauuaccucccuggugaaauaaccgaccucuua2100
caacuggcccguauacccacucucauggcguuugggcgggugucugaacccgagccugcc2160
ucagacgcauaugugcccuacguugccguucccacccaguucgaugacaagccucucauc2220
uccuucccgaucacccuuucagauccugucuaccagaacacccugguaggcgccaucagu2280
ucgaauuucgccaacuaccggggguguauccaaaucacucugacauuuuguggacccaug2340
auggcaagagggaaauuccuacucucguauucucccccaaauggaacgcaaccacagacu2400
cuuucugaagccaugcagugcacauacuccauuugggauauaggcuugaacucuaguugg2460
accuuugucauccccuacaucucgcccagugacuaccgugaaacucgggccaucaccaac2520
ucgguuuacucugccgaugguugguuuagccugcacaagcugaccaaaaucacucuaccg2580
ccugacugcccacagaaacccuguauucucuuuuucgccucugcuggugaggauuacacc2640
cuccgucuccccguugauuguaauccuuccuacguguuccacuccaccgacaacgccgag2700
acugggguuauugaggcggguaacacugacaccgauuucucuggugaacuggcggcuccu2760
ggcucuaaccacacuaaugucaaguuccuguuugaucgaucucgauuacugaauguaauu2820
aagguacuggagaaggacgccgucuucccccguccuuuccccacagaaacaggugcgcag2880
caggacgaugguuacuuuugucuucuaacaccccgcccaacagucgccucccgacccgcc2940
acucguuucggccuguacgucaauccgucugacaguggcguucucgccaacacuucacug3000
gauuucaauuuuuacagcuuggccuguuucacuuacuuuagaucagaccuugaagucacg3060
guggucucacuggagccagaucuggaauuugcuguagggugguuccccucuggcagugag3120
uaccaggcuuccagcuuugucuacgaccaacugcauguacccuaccacuuuaccgggcgc3180
acuccccgcgcuuucaccagcaagggugggaagguaucuuucgugcucccuuggaacucu3240
gucucauccgugcuucccgugcgcuggggggcggcuuccaagcucucuucugccacgcgg3300
gguuugccggcgcaugcugacugggggacuauuuaugcuuucgucccccguccuaaugag3360
aagaaaagcaccgcuguaaagcacguggccguguacguucgguacaagaacgcacgugcc3420
uggugcccuagcaugcuucccuuucgcagcuacaagcagaagaugcugaugcaaucuggc3480
gauaucgagaccaauccuggcccugcuucugacaacccaaucuuggaguuucuugaggca3540
gaaaacgaucuagucacucuggccucucucuggaagaugguacacucuguucagcagacc3600
uggagaaaguacgugaagaacgacaacuuuuggcccaauuuacucagugagcuggugggg3660
gaaggcuccaucgccuuggccgccacacuauccaaccaagcuucaguaaaggcucucuug3720
ggccugcauuuucucucccgggggcucaauuacacagacuuuuacuccuuacugauagag3780
aaaugcucuaguuucuucacuguagaaccaccuccuccaccagccgagaaccugaugacc3840
aagcccuccgugaagucgaaauuccgaaagcuguuuaagaugcaagggcccauggacaaa3900
gucaaagacuggaaccaaauagccgccgguuugaagaauuuccaauuuguccgugaccua3960
gucaaagagguggucgacuggcugcaggccuggaucaacaaggagaaagccagcccuguc4020
cuccaguaucaguuagagaugaagaagcucgggccuguggcucuggcucaugaugcuuuc4080
auggcugguucugggcccccucuuagugacgaucagauugaauaucuccagaaccuuaaa4140
ucucuugcccuaacacuggggaagacuaauuuggcccaaagucucaccacuaugaucaau4200
gccaaacaaaguuccgcccaacgagucgaacccguuguggugguccuuagaggcaagccg4260
ggaugcggcaagagcuuggccuccacgcugauugcccaggcuguguccaagcgucucuac4320
ggcucccaaaguguguauucucuucccccggauccagauuucuucgacggauacaaagga4380
caguuuguaaccuugauggaugaucugggacaaaacccggauggacaagauuucuccacc4440
uuuugucagauggugucgaccgcccaauuucuccccaacauggcggaccuugcagagaag4500
gggcgucccuucaccuccaaucuuaucauugcaaccacaaaccucccccacuucagcccu4560
gucaccauugcugauccuuccgcagucucucgucguaucaacuacgaccugacucuagaa4620
guaucugaggccuacaagaagcauacacggcugaauuuugaccuggcuuucaggcgcaca4680
gacgccccccccauuuauccuuuugcugcccaugugcccuucguggacguagcugugcgc4740
uucaaaaauggucaccagaacuuuaaucuccuagaguuggucgacuccauuugugcagac4800
auucgagccaagcaacaaggugcccggaacaugcagacuuuggucuuacagagcccuaau4860
gagaaugaugacacccccgucgacgaggcguuggguagaguucucacccccgcugcgguc4920
gacgaggcgcuugucgaccucgcuccagaggccgauccgguuggccgcuuggcuauucuu4980
gccaagcuaggucuugcccuagcugcggucaccccugguuugauaaucuuggcaguggga5040
cuuuacagguacuucucuggcucugauacagaccaagaugaaacagaaugugaggaaucu5100
accaaagcaccuaggagcgaaaaugcuuaugacggcccgaagaaaaacucuaagcccccu5160
ggagcacucucucuuauggaaaugcaacagcccaacguggacaugggcuuugaggcugcg5220
gucgcuaagaaaguggucguccccauuaccuucaugguucccaacagacccucuggacuu5280
acacaguccgcucuucuuguggccggccggaccuuccuaaucaaugagcacacaugguca5340
aaucccuccuggaccagcuucaccauccguggugaggugcauacucgugaugagccuuuu5400
caaacgguucauuuuacccaccaugguguucccacagaucugaugaugguacgucucgga5460
ccgggcaacucuuucccuaacaaucuagacaaguuuggacuugaccagaugccggcacgc5520
aacucccgugugguuggcguuucgucuaguuacggcaauuucuucuucucugggaauuuc5580
cucggguucguugacuccaucaccucugaacaaggaacuuaugcaagacuuuuuagguac5640
agggugacgacuuacaagggcuggugcggcucggcccuggucugugaggccggugguguc5700
cggcgcaucauuggccugcauucugcuggugccgcugguauuggugccgggaccuacauc5760
ucaaaauuaggacugaucaaagcucugaaacaccucggugaaccucuggcuacaaugcaa5820
ggacugaugacugaguuagagccuggaaucaccguacauguaccccggaaaucuaaauug5880
agaaagacgaccgcacacgcaguguacaaaccggaguuugaaccugcuguguugucaaaa5940
uuugaucccagacugaacaaggauguugacuuagaugagguaauuuggucuaaacacacu6000
gccaacguccccuaucaaccuccuuuguucuacacauacaugucagaguacgcucaucga6060
guuuucuccuucuugggaaaagacaaugacauucugaccgucaaagaagcaauccugggc6120
aucccuggacuagaccccauggauccccacacagcuccgggucugcccuaugccauuaac6180
ggccuucgacguacugaucucgucgacuuugugaacggcacgguagacgcagcgcuggcc6240
augcaaauccagaaauucuuagacggugacuacucugaucaugucuuccaaacuuuucug6300
aaagaugaaaucagacccucagagaagguccgagcgggaaaaacccgcauugucgaugug6360
cccucccuggcgcauugcauugugggcagaaugcugcuugggcgcuuugccgccaaguuu6420
caaucccauccuggcuuucuccuuggcucugcuaucggcucugacccugauguuuucugg6480
accgucauaggggcucagcucgagggaagaaagaacacguaugacguggauuacagugcc6540
uuugacucuucacacggcacuggcuccuucgaggcucucaucucucacuuuuucaccgug6600
gacaaugguuuuagcccugcgcugggaccguaucucagaucccuggcugucucggugcac6660
gcuuacggcgagcgucgcaucaagauuaccgguggccuccccuccgguugugccgcgacc6720
agcuugcugaacacagugcucaacaaugugaucaucaggacugcucuggcauugacuuac6780
aaggaauuugaguaugacaugguugauaucaucgccuacggugacgaccuucugguuggu6840
acggauuacgaucuggacuucaaugagguggcacgacgcgcugccaaacugggguauaag6900
augacucccgccaacaaagguuccgucuucccuccgacuuccucucucuccgaugcuguu6960
uuucuaaaacgcaaauucguccaaaacaaugauggcuuauacaaaccaguuauggauuua7020
aagaauuuggaagccaugcucuccuacuucaaaccaggaacacuacucgagaagcugcaa7080
ucuguuucuauguuggcucaacauucuggaaaagaagaauaugauagauugaugcacccc7140
uucgcugacuacggugccguaccgagucacgaguaccugcaggcaagauggagggccuug7200
uucgauugacccagauagcccaaggcgcuucggugcugccggcgauucugggagaacuca7260
gucggaacagaaaagggaaaaaaaaaawaaaaaaaaraaaaaaacaaaaaauaaaaacaa7320
aaauaaacacgaccucacuacgagagcugagacugc7356
<210>11
<211>7310
<212>rna
<213>塞内卡病毒
<400>11
uuugaaauggggggcugggcccugaugcccaguccuuccuuuccccuuccgggggguuaa60
ccggcuguguuugcuagaggcacagaggggcaacauccaaccugcuuuugcggggaacgg120
ugcggcuccgauuccugcgucgccaaagguguuagcgcacccaaacggcgcaccuaccaa180
uguuauugguguggucugcgaguucuagccuacucguuucucccccgaccauucacucac240
ccacgaaaaguguguuguaaccauaagauuuaacccccgcacgggaugugcgauaaccgu300
aagacuggcucaagcgcggaaagcgcuguaaccacaugcuguuagucccuuuauggcugc360
aagauggcuacccaccucggaucacugaacuggagcucgacccuccuuaguaagggaacc420
gagaggccuucgugcaacaagcuccgacacagaguccacgugacugcuaccaccaugagu480
acaugguucuccccucucgacccaggacuucuuuuugaauauccacggcucgauccagag540
gguggggcaugaccccuagcauagcgagcuacagcgggaacuguagcuaggccuuagcgu600
gccuuggauacugccugauagggcgacggccuagucgugucgguucuauagguagcacau660
acaaauaugcagaacucucauuuuucuuucgauacagccucuggcaccuuugaagaugua720
accggaacaaaagucaagaucguugaauaccccagaucggugaacaaugguguuuacgau780
ucgucuacucauuuggagauacugaaccuacagggugaaauugaaauuuuaaggucuuuc840
aaugaauaccaaauucgcgccgccaaacaacaacucggacuggacaucguguacgaacua900
caggguaauguucagacaacgucaaagaaugauuuugauucccguggcaauaaugguaac960
augaccuucaauuacuacgcaaacacuuaucagaauucaguagacuucucgaccuccucg1020
ucggcgucaggcgccggacccgggaacucccggggcggauuagcgggucuccucacaaau1080
uucaguggaaucuugaacccucuuggcuaccucaaagaucacaacaccgaagaaauggaa1140
aacucugcugaucgagucacaacgcaaacggcgggcaacacugccauaaacacgcaauca1200
ucauuggguguguugugugccuacguugaagacccgaccaaaucugauccuccguccagc1260
agcacagaucaacccaccaccacuuucacugccaucgacaggugguacacuggacgucuc1320
aauucuuggacaaaagcuguaaaaaccuucucuuuucaggccgucccgcuucccggggcc1380
uuucugucuaggcagggaggccucaacggaggggccuucacagcuacccuacauagacac1440
uuuuugaugaagugcggguggcaggugcagguccaauguaauuugacacaauuccaccaa1500
ggcgcucuucuuguugccaugguuccugaaaccacccuugaugucaagcccgacgguaag1560
gcaaagagcuuacaggagcugaaugaagaacaguggguggaaaugucugacgauuaccgg1620
accgggaaaaacaugccuuuucagucucuuggcacauacuaucggcccccuaacuggacu1680
uggggucccaauuucaucaaccccuaucaaguaacgguuuucccacaccaaauucugaac1740
gcgagaaccucuaccucgguagacauaaacgucccauacaucggggagacccccacgcaa1800
uccucagagacacagaacuccuggacccuccucguuauggugcucguuccccuagacuau1860
aaggaaggagccacaacugacccagaaauuacauuuucuguaaggccuacaagucccuac1920
uucaaugggcuucgcaaccgcuacacggccgggacggacgaagaacaggggcccauuccu1980
acggcacccagagaaaauucgcuuauguuucucucaacccucccugacgacacugucccu2040
gcuuacgggaaugugcguaccccuccugucaauuaccucccuggugaaauaaccgaccuu2100
uugcaacuggcccgcauacccacucucauggcauuugagcgggugccugaacccgugccu2160
gccucagacacauaugugcccuacguugccguucccacccaguucgaugacaggccucuc2220
aucuccuucccgaucacccuuucagaucccgucuaucagaacacccugguuggcgccauc2280
aguucaaauuucgccaauuaccguggguguauccaaaucacucugacauuuuguggaccc2340
augauggcgagagggaaauuccugcucucguauucucccccaaauggaacgcaaccacag2400
acucuuuccgaagcuaugcagugcacauacucuauuugggacauaggcuugaacucuagu2460
uggaccuucgucguccccuacaucucgcccagugacuaccgugaaacucgagccauuacc2520
aacucgguuuacuccgcugaugguugguuuagccugcacaaguugaccaaaauuacucua2580
ccaccugacuguccgcaaagucccugcauucucuuuuucgcuucugcuggugaggauuac2640
acucuccgucuccccguugauuguaauccuuccuauguguuccacuccaccgacaacgcc2700
gagaccgggguuauugaggcggguaacacugacaccgauuucucuggugaacuggcggcu2760
ccuggcucuaaccacacuaaugucaaguuccuguuugaucgaucucgauuauugaaugua2820
aucaagguacuggagaaggacgccguuuucccccgcccuuucccuacacaagaaggugcg2880
cagcaggaugaugguuacuuuugucuucugaccccccgcccaacagucgcuucccgaccc2940
gccacucguuucggccuguacgccaauccguccggcagugguguucuugcuaacacuuca3000
cuggacuucaauuuuuauagcuuggccuguuucacuuacuuuagaucggaccuugagguu3060
acgguggucucacuagagccggaucuggaauuugcuguagggugguuuccuucuggcagu3120
gaauaccaggcuuccagcuuugucuacgaccagcugcaugugcccuuccacuuuacuggg3180
cgcacuccccgcgcuuucgcuagcaagggugggaagguaucuuucgugcucccuuggaac3240
ucugucucgucugugcuccccgugcgcugggggggggcuuccaagcucucuucugcuacg3300
cggggucuaccggcgcaugcugauugggggacuauuuacgccuuugucccccguccuaau3360
gagaagaaaagcaccgcuguaaaacacguggccguguacauucgguacaagaacgcacgu3420
gccuggugccccagcaugcuucccuuucgcagcuacaagcagaagaugcugaugcaaucu3480
ggcgauaucgagaccaauccugguccugcuucugacaacccaauuuuggaguuucuugaa3540
gcagaaaaugaucuagucacucuggccucucucuggaagauggugcacucuguucaacag3600
accuggagaaaguaugugaagaacgaugauuuuuggcccaauuuacucagcgagcuagug3660
ggggaaggcucugucgccuuggccgccacgcuauccaaccaagcuucaguaaaggcucuu3720
uugggccugcacuuucucucucgggggcucaauuacacugacuuuuacucuuuacugaua3780
gagaaaugcucuaguuucuuuaccguagaaccaccuccuccaccagcugaaaaccugaug3840
accaagcccucagugaagucgaaauuccgaaaacuguuuaagaugcaaggacccauggac3900
aaagucaaagacuggaaccaaauagcugccggcuugaagaauuuucaauuuguucgugac3960
cuagucaaagagguggucgauuggcugcaggccuggaucaacaaagagaaagccagcccu4020
guccuccaguaccaguuggagaugaagaagcucgggccuguggccuuggcucaugacgcu4080
uucauggcugguuccgggcccccucuuagcgacgaccagauugaauaccuccagaaccuc4140
aaaucucuugcccuaacacuggggaagacuaauuuggcccaaagucucaccacuaugauc4200
aaugccaaacaaaguucagcccaacgaguugaacccguuguggugguccuuagaggcaag4260
ccgggaugcggcaagagcuuggccucuacguugauugcccaggcuguguccaagcgccuc4320
uauggcucccaaaguguauauucucuucccccagauccagauuucuucgauggauacaaa4380
ggacaguucgugaccuugauggaugauuugggacaaaacccggauggacaagauuucucc4440
accuuuugucagauggugucgaccgcccaauuucuccccaacauggcggaccuugcagag4500
aaagggcgucccuuuaccuccaaucucaucauugcaacuacaaaucucccccacuucagu4560
ccugucaccauugcugauccuucugcagucucucgccguaucaacuacgaucugacucua4620
gaaguaucugaggccuacaagaaacacacacggcugaauuuugacuuggcuuucaggcgc4680
acagacgccccccccauuuauccuuuugcugcccaugugcccuuuguggacguagcugug4740
cgcuucaaaaauggucaccagaauuuuaaucuccuagaguuggucgauuccauuuguaca4800
gacauucgagccaagcaacaaggugcccgaaacaugcagacucugguucuacagagcccc4860
aacgagaaugaugacacccccgucgacgaggcguuggguagaguucucucccccgcugcg4920
gucgaugaggcgcuugucgaccucacuccagaggccgacccgguuggccguuuggcuauu4980
cuugccaagcuaggucuugcccuagcugcggucaccccuggucugauaaucuuggcagug5040
ggacucuacagguacuucucuggcucugaugcagaccaagaagaaacagaaagugaggga5100
ucugucaaggcacccaggagcgaaaaugcuuaugacggcccgaagaaaaacucuaagccc5160
ccuggagcacucucucucauggaaaugcaacagcccaacguggacaugggcuuugaggcu5220
gcggucgcuaagaaaguggucguccccauuaccuucaugguucccaacagaccuucuggg5280
cuuacacaguccgcucuucuggugaccggccggaccuuccuaaucaaugaacauacaugg5340
uccaaucccuccuggaccagcuucacaauccgcggugagguacacacucgugaugagccc5400
uuccaaacgguucauuucacucaccacgguauucccacagaucugaugaugguacgucuc5460
ggaccgggcaauucuuucccuaacaaucuagacaaguuuggacuugaccagaugccggca5520
cgcaacucccgugugguuggcguuucguccaguuacggaaacuucuucuucucuggaaau5580
uuccucggauuuguugauuccaucaccucugaacaaggaacuuacgcaagacucuuuagg5640
uacagggugacgaccuacaaaggauggugcggcucggcccuggucugugaggccgguggc5700
guccgacgcaucauuggccugcauucugcuggcgccgccgguaucggcgccgggaccuau5760
aucucaaaauuaggacuaaucaaagcccugaaacaccucggugaaccuuuggccacaaug5820
caaggacugaugacugaauuagagccuggaaucaccguacauguaccccggaaauccaaa5880
uugagaaagacgaccgcacacgcgguguacaaaccggaguuugagccugcuguguuguca5940
aaauuugaucccagacugaacaaggauguugacuuggaugaaguaauuuggucuaaacac6000
acugccaaugucccuuaccaaccuccuuuguucuacacauacaugucagaguacgcucau6060
cgagucuucuccuucuuggggaaagacaaugacauucugaccgucaaagaagcaauucug6120
ggcauccccggacuagaccccauggauccccacacagcuccgggucugccuuacgccauc6180
aacggccuucgacguacugaucucgucgauuuugugaacgguacaguagaugcggcgcug6240
gcuguacaaauccagaaauucuuagacggugacuacucugaccaugucuuccaaacuuuu6300
cugaaagaugagaucagacccucagagaaaguccgagcgggaaaaacccgcauuguugau6360
gugcccucccuggcgcauugcauugugggcagaauguugcuugggcgcuuugcugccaag6420
uuucaaucccauccuggcuuucuccucggcucugcuaucgggucugacccugauguuuuc6480
uggaccgucauaggggcucaacucgaggggagaaagaacacguaugacguggacuacagu6540
gccuuugacucuucacacggcacuggcuccuucgaggcucucaucucucacuuuuucacc6600
guggacaaugguuuuagcccugcgcugggaccguaucucagaucccuggcugucucggug6660
cacgcuuacggcgagcgucgcaucaagauuaccgguggccuccccuccgguugugccgcg6720
accagccugcugaacacagugcucaacaaugugaucaucaggacugcucuggcauugacu6780
uacaaggaauuugaauaugacaugguugauaucaucgccuacggugacgaccuucugguu6840
ggcacggauuacgaucuggacuucaaugagguggcacgacgcgcugccaaguugggguau6900
aagaugacuccugccaacaaggguucugucuucccuccgacuuccucucuuuccgaugcu6960
guuuuucuaaagcgcaaauucguccaaaacaacgacggcuuauacaaaccaguuauggau7020
uuaaagaauuuggaagccaugcucuccuacuucaaaccaggaacacuacucgagaagcug7080
caaucuguuucuauguuggcucaacauucuggaaaagaagaauaugauagauugaugcac7140
cccuucgcugacuacggugccguaccgagucacgaguaccugcaggcaagauggagggcc7200
uuguucgacugacccagauagcccaaggcgcuucggugcugccggcgauucugggagaac7260
ucagucggaacagaaaagggaaaaaaaaaaaaaaaaaaaaaaaaaaaaaa7310
<210>12
<211>7310
<212>rna
<213>塞内卡病毒
<400>12
uuugaaauggggggcugggcccugaugcccaguccuuccuuuccccuuccgggggguuaa60
ccggcuguguuugcuagaggcacagaggggcaacauccaaccugcuuuugcggggaacgg120
ugcggcuccgauuccugcgucgccaaagguguuagcgcacccaaacggcgcaccuaccaa180
uguuauugguguggucugcgaguucuagccuacucguuucucccccgaccauucacucac240
ccacgaaaaguguguuguaaccauaagauuuaacccccgcacgggaugugcgauaaccgu300
aagacuggcucaagcgcggaaagcgcuguaaccacaugcuguuagucccuuuauggcugc360
aagauggcuacccaccucggaucacugaacuggagcucgacccuccuuaguaagggaacc420
gagaggccuucgugcaacaagcuccgacacagaguccacgugacugcuaccaccaugagu480
acaugguucuccccucucgacccaggacuucuuuuugaauauccacggcucgauccagag540
gguggggcaugaccccuagcauagcgagcuacagcgggaacuguagcuaggccuuagcgu600
gccuuggauacugccugauagggcgacggccuagucgugucgguucuauagguagcacau660
acaaauaugcagaacucucauuuuucuuucgauacagccucuggcaccuuugaagaugua720
accggaacaaaagucaagaucguugaauaccccagaucggugaacaaugguguuuacgau780
ucgucuacucauuuggagauacugaaccuacagggugaaauugaaauuuuaaggucuuuc840
aaugaauaccaaauucgcgccgccaaacaacaacucggacuggacaucguguacgaacua900
caggguaauguucagacaacgucaaagaaugauuuugauucccguggcaauaaugguaac960
augaccuucaauuacuacgcaaacacuuaucagaauucaguagacuucucgaccuccucg1020
ucggcgucaggcgccggacccgggaacucccggggcggauuagcgggucuccucacaaau1080
uucaguggaaucuugaacccucuuggcuaccucaaagaucacaacaccgaagaaauggaa1140
aacucugcugaucgagucacaacgcaaacggcgggcaacacugccauaaacacgcaauca1200
ucauuggguguguugugugccuacguugaagacccgaccaaaucugauccuccguccagc1260
agcacagaucaacccaccaccacuuucacugccaucgacaggugguacacuggacgucuc1320
aauucuuggacaaaagcuguaaaaaccuucucuuuucaggccgucccgcuucccggggcc1380
uuucugucuaggcagggaggccucaacggaggggccuucacagcuacccuacauagacac1440
uuuuugaugaagugcggguggcaggugcagguccaauguaauuugacacaauuccaccaa1500
ggcgcucuucuuguugccaugguuccugaaaccacccuugaugucaagcccgacgguaag1560
gcaaagagcuuacaggagcugaaugaagaacaguggguggaaaugucugacgauuaccgg1620
accgggaaaaacaugccuuuucagucucuuggcacauacuaucggcccccuaacuggacu1680
uggggucccaauuucaucaaccccuaucaaguaacgguuuucccacaccaaauucugaac1740
gcgagaaccucuaccucgguagacauaaacgucccauacaucggggagacccccacgcaa1800
uccucagagacacagaacuccuggacccuccucguuauggugcucguuccccuagacuau1860
aaggaaggagccacaacugacccagaaauuacauuuucuguaaggccuacaagucccuac1920
uucaaugggcuucgcaaccgcuacacggccgggacggacgaagaacaggggcccauuccu1980
acggcacccagagaaaauucgcuuauguuucucucaacccucccugacgacacugucccu2040
gcuuacgggaaugugcguaccccuccugucaauuaccucccuggugaaauaaccgaccuu2100
uugcaacuggcccgcauacccacucucauggcauuugagcgggugccugaacccgugccu2160
gccucagacacauaugugcccuacguugccguucccacccaguucgaugacaggccucuc2220
aucuccuucccgaucacccuuucagaucccgucuaucagaacacccugguuggcgccauc2280
aguucaaauuucgccaauuaccguggguguauccaaaucacucugacauuuuguggaccc2340
augauggcgagagggaaauuccugcucucguauucucccccaaauggaacgcaaccacag2400
acucuuuccgaagcuaugcagugcacauacucuauuugggacauaggcuugaacucuagu2460
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aacucgguuuacuccgcugaugguugguuuagccugcacaaguugaccaaaauuacucua2580
ccaccugacuguccgcaaagucccugcauucucuuuuucgcuucugcuggugaggauuac2640
acucuccgucuccccguugauuguaauccuuccuauguguuccacuccaccgacaacgcc2700
gagaccgggguuauugaggcggguaacacugacaccgauuucucuggugaacuggcggcu2760
ccuggcucuaaccacacuaaugucaaguuccuguuugaucgaucucgauuauugaaugua2820
aucaagguacuggagaaggacgccguuuucccccgcccuuucccuacacaagaaggugcg2880
cagcaggaugaugguuacuuuugucuucugaccccccgcccaacagucgcuucccgaccc2940
gccacucguuucggccuguacgccaauccguccggcagugguguucuugcuaacacuuca3000
cuggacuucaauuuuuauagcuuggccuguuucacuuacuuuagaucggaccuugagguu3060
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gaauaccaggcuuccagcuuugucuacgaccagcugcaugugcccuuccacuuuacuggg3180
cgcacuccccgcgcuuucgcuagcaagggugggaagguaucuuucgugcucccuuggaac3240
ucugucucgucugugcuccccgugcgcugggggggggcuuccaagcucucuucugcuacg3300
cggggucuaccggcgcaugcugauugggggacuauuuacgccuuugucccccguccuaau3360
gagaagaaaagcaccgcuguaaaacacguggccguguacauucgguacaagaacgcacgu3420
gccuggugccccagcaugcuucccuuucgcagcuacaagcagaagaugcugaugcaaucu3480
ggcgauaucgagaccaauccugguccugcuucugacaacccaauuuuggaguuucuugaa3540
gcagaaaaugaucuagucacucuggccucucucuggaagauggugcacucuguucaacag3600
accuggagaaaguaugugaagaacgaugauuuuuggcccaauuuacucagcgagcuagug3660
ggggaaggcucugucgccuuggccgccacgcuauccaaccaagcuucaguaaaggcucuu3720
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gagaaaugcucuaguuucuuuaccguagaaccaccuccuccaccagcugaaaaccugaug3840
accaagcccucagugaagucgaaauuccgaaaacuguuuaagaugcaaggacccauggac3900
aaagucaaagacuggaaccaaauagcugccggcuugaagaauuuucaauuuguucgugac3960
cuagucaaagagguggucgauuggcugcaggccuggaucaacaaagagaaagccagcccu4020
guccuccaguaccaguuggagaugaagaagcucgggccuguggccuuggcucaugacgcu4080
uucauggcugguuccgggcccccucuuagcgacgaccagauugaauaccuccagaaccuc4140
aaaucucuugcccuaacacuggggaagacuaauuuggcccaaagucucaccacuaugauc4200
aaugccaaacaaaguucagcccaacgaguugaacccguuguggugguccuuagaggcaag4260
ccgggaugcggcaagagcuuggccucuacguugauugcccaggcuguguccaagcgccuc4320
uauggcucccaaaguguauauucucuucccccagauccagauuucuucgauggauacaaa4380
ggacaguucgugaccuugauggaugauuugggacaaaacccggauggacaagauuucucc4440
accuuuugucagauggugucgaccgcccaauuucuccccaacauggcggaccuugcagag4500
aaagggcgucccuuuaccuccaaucucaucauugcaacuacaaaucucccccacuucagu4560
ccugucaccauugcugauccuucugcagucucucgccguaucaacuacgaucugacucua4620
gaaguaucugaggccuacaagaaacacacacggcugaauuuugacuuggcuuucaggcgc4680
acagacgccccccccauuuauccuuuugcugcccaugugcccuuuguggacguagcugug4740
cgcuucaaaaauggucaccagaauuuuaaucuccuagaguuggucgauuccauuuguaca4800
gacauucgagccaagcaacaaggugcccgaaacaugcagacucugguucuacagagcccc4860
aacgagaaugaugacacccccgucgacgaggcguuggguagaguucucucccccgcugcg4920
gucgaugaggcgcuugucgaccucacuccagaggccgacccgguuggccguuuggcuauu4980
cuugccaagcuaggucuugcccuagcugcggucaccccuggucugauaaucuuggcagug5040
ggacucuacagguacuucucuggcucugaugcagaccaagaagaaacagaaagugaggga5100
ucugucaaggcacccaggagcgaaaaugcuuaugacggcccgaagaaaaacucuaagccc5160
ccuggagcacucucucucauggaaaugcaacagcccaacguggacaugggcuuugaggcu5220
gcggucgcuaagaaaguggucguccccauuaccuucaugguucccaacagaccuucuggg5280
cuuacacaguccgcucuucuggugaccggccggaccuuccuaaucaaugaacauacaugg5340
uccaaucccuccuggaccagcuucacaauccgcggugagguacacacucgugaugagccc5400
uuccaaacgguucauuucacucaccacgguauucccacagaucugaugaugguacgucuc5460
ggaccgggcaauucuuucccuaacaaucuagacaaguuuggacuugaccagaugccggca5520
cgcaacucccgugugguuggcguuucguccaguuacggaaacuucuucuucucuggaaau5580
uuccucggauuuguugauuccaucaccucugaacaaggaacuuacgcaagacucuuuagg5640
uacagggugacgaccuacaaaggauggugcggcucggcccuggucugugaggccgguggc5700
guccgacgcaucauuggccugcauucugcuggcgccgccgguaucggcgccgggaccuau5760
aucucaaaauuaggacuaaucaaagcccugaaacaccucggugaaccuuuggccacaaug5820
caaggacugaugacugaauuagagccuggaaucaccguacauguaccccggaaauccaaa5880
uugagaaagacgaccgcacacgcgguguacaaaccggaguuugagccugcuguguuguca5940
aaauuugaucccagacugaacaaggauguugacuuggaugaaguaauuuggucuaaacac6000
acugccaaugucccuuaccaaccuccuuuguucuacacauacaugucagaguacgcucau6060
cgagucuucuccuucuuggggaaagacaaugacauucugaccgucaaagaagcaauucug6120
ggcauccccggacuagaccccauggauccccacacagcuccgggucugccuuacgccauc6180
aacggccuucgacguacugaucucgucgauuuugugaacgguacaguagaugcggcgcug6240
gcuguacaaauccagaaauucuuagacggugacuacucugaccaugucuuccaaacuuuu6300
cugaaagaugagaucagacccucagagaaaguccgagcgggaaaaacccgcauuguugau6360
gugcccucccuggcgcauugcauugugggcagaauguugcuugggcgcuuugcugccaag6420
uuucaaucccauccuggcuuucuccucggcucugcuaucgggucugacccugauguuuuc6480
uggaccgucauaggggcucaacucgaggggagaaagaacacguaugacguggacuacagu6540
gccuuugacucuucacacggcacuggcuccuucgaggcucucaucucucacuuuuucacc6600
guggacaaugguuuuagcccugcgcugggaccguaucucagaucccuggcugucucggug6660
cacgcuuacggcgagcgucgcaucaagauuaccgguggccuccccuccgguugugccgcg6720
accagccugcugaacacagugcucaacaaugugaucaucaggacugcucuggcauugacu6780
uacaaggaauuugaauaugacaugguugauaucaucgccuacggugacgaccuucugguu6840
ggcacggauuacgaucuggacuucaaugagguggcacgacgcgcugccaaguugggguau6900
aagaugacuccugccaacaaggguucugucuucccuccgacuuccucucuuuccgaugcu6960
guuuuucuaaagcgcaaauucguccaaaacaacgacggcuuauacaaaccaguuauggau7020
uuaaagaauuuggaagccaugcucuccuacuucaaaccaggaacacuacucgagaagcug7080
caaucuguuucuauguuggcucaacauucuggaaaagaagaauaugauagauugaugcac7140
cccuucgcugacuacggugccguaccgagucacgaguaccugcaggcaagauggagggcc7200
uuguucgacugacccagauagcccaaggcgcuucggugcugccggcgauucugggagaac7260
ucagucggaacagaaaagggaaaaaaaaaaaaaaaaaaaaaaaaaaaaaa7310
<210>13
<211>7310
<212>rna
<213>塞内卡病毒
<400>13
uuugaaauggggggcugggcccugaugcccaguccuuccuuuccccuuccgggggguuaa60
ccggcuguguuugcuagaggcacagaggggcaacauccaaccugcuuuugcggggaacgg120
ugcggcuccgauuccugcgucgccaaagguguuagcgcacccaaacggcgcaccuaccaa180
uguuauugguguggucugcgaguucuagccuacucguuucucccccgaccauucacucac240
ccacgaaaaguguguuguaaccauaagauuuaacccccgcacgggaugugcgauaaccgu300
aagacuggcucaagcgcggaaagcgcuguaaccacaugcuguuagucccuuuauggcugc360
aagauggcuacccaccucggaucacugaacuggagcucgacccuccuuaguaagggaacc420
gagaggccuucgugcaacaagcuccgacacagaguccacgugacugcuaccaccaugagu480
acaugguucuccccucucgacccaggacuucuuuuugaauauccacggcucgauccagag540
gguggggcaugaccccuagcauagcgagcuacagcgggaacuguagcuaggccuuagcgu600
gccuuggauacugccugauagggcgacggccuagucgugucgguucuauagguagcacau660
acaaauaugcagaacucucauuuuucuuucgauacagccucuggcaccuuugaagaugua720
accggaacaaaagucaagaucguugaauaccccagaucggugaacaaugguguuuacgau780
ucgucuacucauuuggagauacugaaccuacagggugaaauugaaauuuuaaggucuuuc840
aaugaauaccaaauucgcgccgccaaacaacaacucggacuggacaucguguacgaacua900
caggguaauguucagacaacgucaaagaaugauuuugauucccguggcaauaaugguaac960
augaccuucaauuacuacgcaaacacuuaucagaauucaguagacuucucgaccuccucg1020
ucggcgucaggcgccggacccgggaacucccggggcggauuagcgggucuccucacaaau1080
uucaguggaaucuugaacccucuuggcuaccucaaagaucacaacaccgaagaaauggaa1140
aacucugcugaucgagucacaacgcaaacggcgggcaacacugccauaaacacgcaauca1200
ucauuggguguguugugugccuacguugaagacccgaccaaaucugauccuccguccagc1260
agcacagaucaacccaccaccacuuucacugccaucgacaggugguacacuggacgucuc1320
aauucuuggacaaaagcuguaaaaaccuucucuuuucaggccgucccgcuucccggggcc1380
uuucugucuaggcagggaggccucaacggaggggccuucacagcuacccuacauagacac1440
uuuuugaugaagugcggguggcaggugcagguccaauguaauuugacacaauuccaccaa1500
ggcgcucuucuuguugccaugguuccugaaaccacccuugaugucaagcccgacgguaag1560
gcaaagagcuuacaggagcugaaugaagaacaguggguggaaaugucugacgauuaccgg1620
accgggaaaaacaugccuuuucagucucuuggcacauacuaucggcccccuaacuggacu1680
uggggucccaauuucaucaaccccuaucaaguaacgguuuucccacaccaaauucugaac1740
gcgagaaccucuaccucgguagacauaaacgucccauacaucggggagacccccacgcaa1800
uccucagagacacagaacuccuggacccuccucguuauggugcucguuccccuagacuau1860
aaggaaggagccacaacugacccagaaauuacauuuucuguaaggccuacaagucccuac1920
uucaaugggcuucgcaaccgcuacacggccgggacggacgaagaacaggggcccauuccu1980
acggcacccagagaaaauucgcuuauguuucucucaacccucccugacgacacugucccu2040
gcuuacgggaaugugcguaccccuccugucaauuaccucccuggugaaauaaccgaccuu2100
uugcaacuggcccgcauacccacucucauggcauuugagcgggugccugaacccgugccu2160
gccucagacacauaugugcccuacguugccguucccacccaguucgaugacaggccucuc2220
aucuccuucccgaucacccuuucagaucccgucuaucagaacacccugguuggcgccauc2280
aguucaaauuucgccaauuaccguggguguauccaaaucacucugacauuuuguggaccc2340
augauggcgagagggaaauuccugcucucguauucucccccaaauggaacgcaaccacag2400
acucuuuccgaagcuaugcagugcacauacucuauuugggacauaggcuugaacucuagu2460
uggaccuucgucguccccuacaucucgcccagugacuaccgugaaacucgagccauuacc2520
aacucgguuuacuccgcugaugguugguuuagccugcacaaguugaccaaaauuacucua2580
ccaccugacuguccgcaaagucccugcauucucuuuuucgcuucugcuggugaggauuac2640
acucuccgucuccccguugauuguaauccuuccuauguguuccacuccaccgacaacgcc2700
gagaccgggguuauugaggcggguaacacugacaccgauuucucuggugaacuggcggcu2760
ccuggcucuaaccacacuaaugucaaguuccuguuugaucgaucucgauuauugaaugua2820
aucaagguacuggagaaggacgccguuuucccccgcccuuucccuacacaagaaggugcg2880
cagcaggaugaugguuacuuuugucuucugaccccccgcccaacagucgcuucccgaccc2940
gccacucguuucggccuguacgccaauccguccggcagugguguucuugcuaacacuuca3000
cuggacuucaauuuuuauagcuuggccuguuucacuuacuuuagaucggaccuugagguu3060
acgguggucucacuagagccggaucuggaauuugcuguagggugguuuccuucuggcagu3120
gaauaccaggcuuccagcuuugucuacgaccagcugcaugugcccuuccacuuuacuggg3180
cgcacuccccgcgcuuucgcuagcaagggugggaagguaucuuucgugcucccuuggaac3240
ucugucucgucugugcuccccgugcgcugggggggggcuuccaagcucucuucugcuacg3300
cggggucuaccggcgcaugcugauugggggacuauuuacgccuuugucccccguccuaau3360
gagaagaaaagcaccgcuguaaaacacguggccguguacauucgguacaagaacgcacgu3420
gccuggugccccagcaugcuucccuuucgcagcuacaagcagaagaugcugaugcaaucu3480
ggcgauaucgagaccaauccugguccugcuucugacaacccaauuuuggaguuucuugaa3540
gcagaaaaugaucuagucacucuggccucucucuggaagauggugcacucuguucaacag3600
accuggagaaaguaugugaagaacgaugauuuuuggcccaauuuacucagcgagcuagug3660
ggggaaggcucugucgccuuggccgccacgcuauccaaccaagcuucaguaaaggcucuu3720
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gagaaaugcucuaguuucuuuaccguagaaccaccuccuccaccagcugaaaaccugaug3840
accaagcccucagugaagucgaaauuccgaaaacuguuuaagaugcaaggacccauggac3900
aaagucaaagacuggaaccaaauagcugccggcuugaagaauuuucaauuuguucgugac3960
cuagucaaagagguggucgauuggcugcaggccuggaucaacaaagagaaagccagcccu4020
guccuccaguaccaguuggagaugaagaagcucgggccuguggccuuggcucaugacgcu4080
uucauggcugguuccgggcccccucuuagcgacgaccagauugaauaccuccagaaccuc4140
aaaucucuugcccuaacacuggggaagacuaauuuggcccaaagucucaccacuaugauc4200
aaugccaaacaaaguucagcccaacgaguugaacccguuguggugguccuuagaggcaag4260
ccgggaugcggcaagagcuuggccucuacguugauugcccaggcuguguccaagcgccuc4320
uauggcucccaaaguguauauucucuucccccagauccagauuucuucgauggauacaaa4380
ggacaguucgugaccuugauggaugauuugggacaaaacccggauggacaagauuucucc4440
accuuuugucagauggugucgaccgcccaauuucuccccaacauggcggaccuugcagag4500
aaagggcgucccuuuaccuccaaucucaucauugcaacuacaaaucucccccacuucagu4560
ccugucaccauugcugauccuucugcagucucucgccguaucaacuacgaucugacucua4620
gaaguaucugaggccuacaagaaacacacacggcugaauuuugacuuggcuuucaggcgc4680
acagacgccccccccauuuauccuuuugcugcccaugugcccuuuguggacguagcugug4740
cgcuucaaaaauggucaccagaauuuuaaucuccuagaguuggucgauuccauuuguaca4800
gacauucgagccaagcaacaaggugcccgaaacaugcagacucugguucuacagagcccc4860
aacgagaaugaugacacccccgucgacgaggcguuggguagaguucucucccccgcugcg4920
gucgaugaggcgcuugucgaccucacuccagaggccgacccgguuggccguuuggcuauu4980
cuugccaagcuaggucuugcccuagcugcggucaccccuggucugauaaucuuggcagug5040
ggacucuacagguacuucucuggcucugaugcagaccaagaagaaacagaaagugaggga5100
ucugucaaggcacccaggagcgaaaaugcuuaugacggcccgaagaaaaacucuaagccc5160
ccuggagcacucucucucauggaaaugcaacagcccaacguggacaugggcuuugaggcu5220
gcggucgcuaagaaaguggucguccccauuaccuucaugguucccaacagaccuucuggg5280
cuuacacaguccgcucuucuggugaccggccggaccuuccuaaucaaugaacauacaugg5340
uccaaucccuccuggaccagcuucacaauccgcggugagguacacacucgugaugagccc5400
uuccaaacgguucauuucacucaccacgguauucccacagaucugaugaugguacgucuc5460
ggaccgggcaauucuuucccuaacaaucuagacaaguuuggacuugaccagaugccggca5520
cgcaacucccgugugguuggcguuucguccaguuacggaaacuucuucuucucuggaaau5580
uuccucggauuuguugauuccaucaccucugaacaaggaacuuacgcaagacucuuuagg5640
uacagggugacgaccuacaaaggauggugcggcucggcccuggucugugaggccgguggc5700
guccgacgcaucauuggccugcauucugcuggcgccgccgguaucggcgccgggaccuau5760
aucucaaaauuaggacuaaucaaagcccugaaacaccucggugaaccuuuggccacaaug5820
caaggacugaugacugaauuagagccuggaaucaccguacauguaccccggaaauccaaa5880
uugagaaagacgaccgcacacgcgguguacaaaccggaguuugagccugcuguguuguca5940
aaauuugaucccagacugaacaaggauguugacuuggaugaaguaauuuggucuaaacac6000
acugccaaugucccuuaccaaccuccuuuguucuacacauacaugucagaguacgcucau6060
cgagucuucuccuucuuggggaaagacaaugacauucugaccgucaaagaagcaauucug6120
ggcauccccggacuagaccccauggauccccacacagcuccgggucugccuuacgccauc6180
aacggccuucgacguacugaucucgucgauuuugugaacgguacaguagaugcggcgcug6240
gcuguacaaauccagaaauucuuagacggugacuacucugaccaugucuuccaaacuuuu6300
cugaaagaugagaucagacccucagagaaaguccgagcgggaaaaacccgcauuguugau6360
gugcccucccuggcgcauugcauugugggcagaauguugcuugggcgcuuugcugccaag6420
uuucaaucccauccuggcuuucuccucggcucugcuaucgggucugacccugauguuuuc6480
uggaccgucauaggggcucaacucgaggggagaaagaacacguaugacguggacuacagu6540
gccuuugacucuucacacggcacuggcuccuucgaggcucucaucucucacuuuuucacc6600
guggacaaugguuuuagcccugcgcugggaccguaucucagaucccuggcugucucggug6660
cacgcuuacggcgagcgucgcaucaagauuaccgguggccuccccuccgguugugccgcg6720
accagccugcugaacacagugcucaacaaugugaucaucaggacugcucuggcauugacu6780
uacaaggaauuugaauaugacaugguugauaucaucgccuacggugacgaccuucugguu6840
ggcacggauuacgaucuggacuucaaugagguggcacgacgcgcugccaaguugggguau6900
aagaugacuccugccaacaaggguucugucuucccuccgacuuccucucuuuccgaugcu6960
guuuuucuaaagcgcaaauucguccaaaacaacgacggcuuauacaaaccaguuauggau7020
uuaaagaauuuggaagccaugcucuccuacuucaaaccaggaacacuacucgagaagcug7080
caaucuguuucuauguuggcucaacauucuggaaaagaagaauaugauagauugaugcac7140
cccuucgcugacuacggugccguaccgagucacgaguaccugcaggcaagauggagggcc7200
uuguucgacugacccagauagcccaaggcgcuucggugcugccggcgauucugggagaac7260
ucagucggaacagaaaagggaaaaaaaaaaaaaaaaaaaaaaaaaaaaaa7310
<210>14
<211>7310
<212>rna
<213>塞内卡病毒
<400>14
uuugaaauggggggcugggcccugaugcccaguccuuccuuuccccuuccgggggguuaa60
ccggcuguguuugcuagaggcacagaggggcaacauccaaccugcuuuugcggggaacgg120
ugcggcuccgauuccugcgucgccaaagguguuagcgcacccaaacggcgcaccuaccaa180
uguuauugguguggucugcgaguucuagccuacucguuucucccccgaccauucacucac240
ccacgaaaaguguguuguaaccauaagauuuaacccccgcacgggaugugcgauaaccgu300
aagacuggcucaagcgcggaaagcgcuguaaccacaugcuguuagucccuuuauggcugc360
aagauggcuacccaccucggaucacugaacuggagcucgacccuccuuaguaagggaacc420
gagaggccuucgugcaacaagcuccgacacagaguccacgugacugcuaccaccaugagu480
acaugguucuccccucucgacccaggacuucuuuuugaauauccacggcucgauccagag540
gguggggcaugaccccuagcauagcgagcuacagcgggaacuguagcuaggccuuagcgu600
gccuuggauacugccugauagggcgacggccuagucgugucgguucuauagguagcacau660
acaaauaugcagaacucucauuuuucuuucgauacagccucuggcaccuuugaagaugua720
accggaacaaaagucaagaucguugaauaccccagaucggugaacaaugguguuuacgau780
ucgucuacucauuuggagauacugaaccuacagggugaaauugaaauuuuaaggucuuuc840
aaugaauaccaaauucgcgccgccaaacaacaacucggacuggacaucguguacgaacua900
caggguaauguucagacaacgucaaagaaugauuuugauucccguggcaauaaugguaac960
augaccuucaauuacuacgcaaacacuuaucagaauucaguagacuucucgaccuccucg1020
ucggcgucaggcgccggacccgggaacucccggggcggauuagcgggucuccucacaaau1080
uucaguggaaucuugaacccucuuggcuaccucaaagaucacaacaccgaagaaauggaa1140
aacucugcugaucgagucacaacgcaaacggcgggcaacacugccauaaacacgcaauca1200
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agcacagaucaacccaccaccacuuucacugccaucgacaggugguacacuggacgucuc1320
aauucuuggacaaaagcuguaaaaaccuucucuuuucaggccgucccgcuucccggggcc1380
uuucugucuaggcagggaggccucaacggaggggccuucacagcuacccuacauagacac1440
uuuuugaugaagugcggguggcaggugcagguccaauguaauuugacacaauuccaccaa1500
ggcgcucuucuuguugccaugguuccugaaaccacccuugaugucaagcccgacgguaag1560
gcaaagagcuuacaggagcugaaugaagaacaguggguggaaaugucugacgauuaccgg1620
accgggaaaaacaugccuuuucagucucuuggcacauacuaucggcccccuaacuggacu1680
uggggucccaauuucaucaaccccuaucaaguaacgguuuucccacaccaaauucugaac1740
gcgagaaccucuaccucgguagacauaaacgucccauacaucggggagacccccacgcaa1800
uccucagagacacagaacuccuggacccuccucguuauggugcucguuccccuagacuau1860
aaggaaggagccacaacugacccagaaauuacauuuucuguaaggccuacaagucccuac1920
uucaaugggcuucgcaaccgcuacacggccgggacggacgaagaacaggggcccauuccu1980
acggcacccagagaaaauucgcuuauguuucucucaacccucccugacgacacugucccu2040
gcuuacgggaaugugcguaccccuccugucaauuaccucccuggugaaauaaccgaccuu2100
uugcaacuggcccgcauacccacucucauggcauuugagcgggugccugaacccgugccu2160
gccucagacacauaugugcccuacguugccguucccacccaguucgaugacaggccucuc2220
aucuccuucccgaucacccuuucagaucccgucuaucagaacacccugguuggcgccauc2280
aguucaaauuucgccaauuaccguggguguauccaaaucacucugacauuuuguggaccc2340
augauggcgagagggaaauuccugcucucguauucucccccaaauggaacgcaaccacag2400
acucuuuccgaagcuaugcagugcacauacucuauuugggacauaggcuugaacucuagu2460
uggaccuucgucguccccuacaucucgcccagugacuaccgugaaacucgagccauuacc2520
aacucgguuuacuccgcugaugguugguuuagccugcacaaguugaccaaaauuacucua2580
ccaccugacuguccgcaaagucccugcauucucuuuuucgcuucugcuggugaggauuac2640
acucuccgucuccccguugauuguaauccuuccuauguguuccacuccaccgacaacgcc2700
gagaccgggguuauugaggcggguaacacugacaccgauuucucuggugaacuggcggcu2760
ccuggcucuaaccacacuaaugucaaguuccuguuugaucgaucucgauuauugaaugua2820
aucaagguacuggagaaggacgccguuuucccccgcccuuucccuacacaagaaggugcg2880
cagcaggaugaugguuacuuuugucuucugaccccccgcccaacagucgcuucccgaccc2940
gccacucguuucggccuguacgccaauccguccggcagugguguucuugcuaacacuuca3000
cuggacuucaauuuuuauagcuuggccuguuucacuuacuuuagaucggaccuugagguu3060
acgguggucucacuagagccggaucuggaauuugcuguagggugguuuccuucuggcagu3120
gaauaccaggcuuccagcuuugucuacgaccagcugcaugugcccuuccacuuuacuggg3180
cgcacuccccgcgcuuucgcuagcaagggugggaagguaucuuucgugcucccuuggaac3240
ucugucucgucugugcuccccgugcgcugggggggggcuuccaagcucucuucugcuacg3300
cggggucuaccggcgcaugcugauugggggacuauuuacgccuuugucccccguccuaau3360
gagaagaaaagcaccgcuguaaaacacguggccguguacauucgguacaagaacgcacgu3420
gccuggugccccagcaugcuucccuuucgcagcuacaagcagaagaugcugaugcaaucu3480
ggcgauaucgagaccaauccugguccugcuucugacaacccaauuuuggaguuucuugaa3540
gcagaaaaugaucuagucacucuggccucucucuggaagauggugcacucuguucaacag3600
accuggagaaaguaugugaagaacgaugauuuuuggcccaauuuacucagcgagcuagug3660
ggggaaggcucugucgccuuggccgccacgcuauccaaccaagcuucaguaaaggcucuu3720
uugggccugcacuuucucucucgggggcucaauuacacugacuuuuacucuuuacugaua3780
gagaaaugcucuaguuucuuuaccguagaaccaccuccuccaccagcugaaaaccugaug3840
accaagcccucagugaagucgaaauuccgaaaacuguuuaagaugcaaggacccauggac3900
aaagucaaagacuggaaccaaauagcugccggcuugaagaauuuucaauuuguucgugac3960
cuagucaaagagguggucgauuggcugcaggccuggaucaacaaagagaaagccagcccu4020
guccuccaguaccaguuggagaugaagaagcucgggccuguggccuuggcucaugacgcu4080
uucauggcugguuccgggcccccucuuagcgacgaccagauugaauaccuccagaaccuc4140
aaaucucuugcccuaacacuggggaagacuaauuuggcccaaagucucaccacuaugauc4200
aaugccaaacaaaguucagcccaacgaguugaacccguuguggugguccuuagaggcaag4260
ccgggaugcggcaagagcuuggccucuacguugauugcccaggcuguguccaagcgccuc4320
uauggcucccaaaguguauauucucuucccccagauccagauuucuucgauggauacaaa4380
ggacaguucgugaccuugauggaugauuugggacaaaacccggauggacaagauuucucc4440
accuuuugucagauggugucgaccgcccaauuucuccccaacauggcggaccuugcagag4500
aaagggcgucccuuuaccuccaaucucaucauugcaacuacaaaucucccccacuucagu4560
ccugucaccauugcugauccuucugcagucucucgccguaucaacuacgaucugacucua4620
gaaguaucugaggccuacaagaaacacacacggcugaauuuugacuuggcuuucaggcgc4680
acagacgccccccccauuuauccuuuugcugcccaugugcccuuuguggacguagcugug4740
cgcuucaaaaauggucaccagaauuuuaaucuccuagaguuggucgauuccauuuguaca4800
gacauucgagccaagcaacaaggugcccgaaacaugcagacucugguucuacagagcccc4860
aacgagaaugaugacacccccgucgacgaggcguuggguagaguucucucccccgcugcg4920
gucgaugaggcgcuugucgaccucacuccagaggccgacccgguuggccguuuggcuauu4980
cuugccaagcuaggucuugcccuagcugcggucaccccuggucugauaaucuuggcagug5040
ggacucuacagguacuucucuggcucugaugcagaccaagaagaaacagaaagugaggga5100
ucugucaaggcacccaggagcgaaaaugcuuaugacggcccgaagaaaaacucuaagccc5160
ccuggagcacucucucucauggaaaugcaacagcccaacguggacaugggcuuugaggcu5220
gcggucgcuaagaaaguggucguccccauuaccuucaugguucccaacagaccuucuggg5280
cuuacacaguccgcucuucuggugaccggccggaccuuccuaaucaaugaacauacaugg5340
uccaaucccuccuggaccagcuucacaauccgcggugagguacacacucgugaugagccc5400
uuccaaacgguucauuucacucaccacgguauucccacagaucugaugaugguacgucuc5460
ggaccgggcaauucuuucccuaacaaucuagacaaguuuggacuugaccagaugccggca5520
cgcaacucccgugugguuggcguuucguccaguuacggaaacuucuucuucucuggaaau5580
uuccucggauuuguugauuccaucaccucugaacaaggaacuuacgcaagacucuuuagg5640
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guccgacgcaucauuggccugcauucugcuggcgccgccgguaucggcgccgggaccuau5760
aucucaaaauuaggacuaaucaaagcccugaaacaccucggugaaccuuuggccacaaug5820
caaggacugaugacugaauuagagccuggaaucaccguacauguaccccggaaauccaaa5880
uugagaaagacgaccgcacacgcgguguacaaaccggaguuugagccugcuguguuguca5940
aaauuugaucccagacugaacaaggauguugacuuggaugaaguaauuuggucuaaacac6000
acugccaaugucccuuaccaaccuccuuuguucuacacauacaugucagaguacgcucau6060
cgagucuucuccuucuuggggaaagacaaugacauucugaccgucaaagaagcaauucug6120
ggcauccccggacuagaccccauggauccccacacagcuccgggucugccuuacgccauc6180
aacggccuucgacguacugaucucgucgauuuugugaacgguacaguagaugcggcgcug6240
gcuguacaaauccagaaauucuuagacggugacuacucugaccaugucuuccaaacuuuu6300
cugaaagaugagaucagacccucagagaaaguccgagcgggaaaaacccgcauuguugau6360
gugcccucccuggcgcauugcauugugggcagaauguugcuugggcgcuuugcugccaag6420
uuucaaucccauccuggcuuucuccucggcucugcuaucgggucugacccugauguuuuc6480
uggaccgucauaggggcucaacucgaggggagaaagaacacguaugacguggacuacagu6540
gccuuugacucuucacacggcacuggcuccuucgaggcucucaucucucacuuuuucacc6600
guggacaaugguuuuagcccugcgcugggaccguaucucagaucccuggcugucucggug6660
cacgcuuacggcgagcgucgcaucaagauuaccgguggccuccccuccgguugugccgcg6720
accagccugcugaacacagugcucaacaaugugaucaucaggacugcucuggcauugacu6780
uacaaggaauuugaauaugacaugguugauaucaucgccuacggugacgaccuucugguu6840
ggcacggauuacgaucuggacuucaaugagguggcacgacgcgcugccaaguugggguau6900
aagaugacuccugccaacaaggguucugucuucccuccgacuuccucucuuuccgaugcu6960
guuuuucuaaagcgcaaauucguccaaaacaacgacggcuuauacaaaccaguuauggau7020
uuaaagaauuuggaagccaugcucuccuacuucaaaccaggaacacuacucgagaagcug7080
caaucuguuucuauguuggcucaacauucuggaaaagaagaauaugauagauugaugcac7140
cccuucgcugacuacggugccguaccgagucacgaguaccugcaggcaagauggagggcc7200
uuguucgacugacccagauagcccaaggcgcuucggugcugccggcgauucugggagaac7260
ucagucggaacagaaaagggaaaaaaaaaaaaaaaaaaaaaaaaaaaaaa7310
<210>15
<211>2181
<212>prt
<213>塞内卡病毒
<400>15
metglnasnserhispheserpheaspthralaserglythrpheglu
151015
aspvalthrglythrlysvallysilevalglutyrproargserval
202530
asnasnglyvaltyraspserserthrhisleugluileleuasnleu
354045
glnglygluilegluileleuargserpheasnglutyrglnilearg
505560
alaalalysglnglnleuglyleuaspilevaltyrgluleuglngly
65707580
asnvalglnthrthrserlysasnasppheaspserargglyasnasn
859095
glyasnmetthrpheasntyrtyralaasnthrtyrglnasnserval
100105110
asppheserthrserserseralaserglyalaglyproglyasnser
115120125
argglyglyleualaglyleuleuthrasnpheserglyileleuasn
130135140
proleuglytyrleulysasphisasnthrgluglumetgluasnser
145150155160
alaaspargvalthrthrglnthralaglyasnthralaileasnthr
165170175
glnserserleuglyvalleucysalatyrvalgluaspprothrlys
180185190
seraspproproserserserthraspglnprothrthrthrphethr
195200205
alaileaspargtrptyrthrglyargleuasnsertrpthrlysala
210215220
vallysthrpheserpheglnalavalproleuproglyalapheleu
225230235240
serargglnglyglyleuasnglyglyalaphethralathrleuhis
245250255
arghispheleumetlyscysglytrpglnvalglnvalglncysasn
260265270
leuthrglnphehisglnglyalaleuleuvalalametvalproglu
275280285
thrthrleuaspvallysproaspglylysalalysserleuglnglu
290295300
leuasnglugluglntrpvalglumetseraspasptyrargthrgly
305310315320
lysasnmetpropheglnserleuglythrtyrtyrargproproasn
325330335
trpthrtrpglyproasnpheileasnprotyrglnvalthrvalphe
340345350
prohisglnileleuasnalaargthrserthrservalaspileasn
355360365
valprotyrileglygluthrprothrglnsersergluthrglnasn
370375380
sertrpthrleuleuvalmetvalleuvalproleuasptyrlysglu
385390395400
glyalathrthraspprogluilethrpheservalargprothrser
405410415
protyrpheasnglyleuargasnargtyrthralaglythraspglu
420425430
gluglnglyproileprothralaproarggluasnserleumetphe
435440445
leuserthrleuproaspaspthrvalproalatyrglyasnvalarg
450455460
thrproprovalasntyrleuproglygluilethraspleuleugln
465470475480
leualaargileprothrleumetalaphegluargvalproglupro
485490495
valproalaseraspthrtyrvalprotyrvalalavalprothrgln
500505510
pheaspaspargproleuileserpheproilethrleuserasppro
515520525
valtyrglnasnthrleuvalglyalaileserserasnphealaasn
530535540
tyrargglycysileglnilethrleuthrphecysglyprometmet
545550555560
alaargglylyspheleuleusertyrserproproasnglythrgln
565570575
proglnthrleuserglualametglncysthrtyrseriletrpasp
580585590
ileglyleuasnsersertrpthrphevalvalprotyrileserpro
595600605
serasptyrarggluthrargalailethrasnservaltyrserala
610615620
aspglytrppheserleuhislysleuthrlysilethrleupropro
625630635640
aspcysproglnserprocysileleuphephealaseralaglyglu
645650655
asptyrthrleuargleuprovalaspcysasnprosertyrvalphe
660665670
hisserthraspasnalagluthrglyvalileglualaglyasnthr
675680685
aspthrasppheserglygluleualaalaproglyserasnhisthr
690695700
asnvallyspheleupheaspargserargleuleuasnvalilelys
705710715720
valleuglulysaspalavalpheproargpropheprothrglnglu
725730735
glyalaglnglnaspaspglytyrphecysleuleuthrproargpro
740745750
thrvalalaserargproalathrargpheglyleutyralaasnpro
755760765
serglyserglyvalleualaasnthrserleuasppheasnphetyr
770775780
serleualacysphethrtyrpheargseraspleugluvalthrval
785790795800
valserleugluproaspleugluphealavalglytrppheproser
805810815
glyserglutyrglnalaserserphevaltyraspglnleuhisval
820825830
prophehisphethrglyargthrproargalaphealaserlysgly
835840845
glylysvalserphevalleuprotrpasnservalserservalleu
850855860
provalargtrpglyglyalaserlysleuserseralathrarggly
865870875880
leuproalahisalaasptrpglythriletyralaphevalproarg
885890895
proasnglulyslysserthralavallyshisvalalavaltyrile
900905910
argtyrlysasnalaargalatrpcysprosermetleuprophearg
915920925
sertyrlysglnlysmetleumetglnserglyaspilegluthrasn
930935940
proglyproalaseraspasnproileleuglupheleuglualaglu
945950955960
asnaspleuvalthrleualaserleutrplysmetvalhisserval
965970975
glnglnthrtrparglystyrvallysasnaspaspphetrpproasn
980985990
leuleusergluleuvalglygluglyservalalaleualaalathr
99510001005
leuserasnglnalaservallysalaleuleuglyleuhisphe
101010151020
leuserargglyleuasntyrthraspphetyrserleuleuile
102510301035
glulyscysserserphephethrvalglupropropropropro
104010451050
alagluasnleumetthrlysproservallysserlysphearg
105510601065
lysleuphelysmetglnglyprometasplysvallysasptrp
107010751080
asnglnilealaalaglyleulysasnpheglnphevalargasp
108510901095
leuvallysgluvalvalasptrpleuglnalatrpileasnlys
110011051110
glulysalaserprovalleuglntyrglnleuglumetlyslys
111511201125
leuglyprovalalaleualahisaspalaphemetalaglyser
113011351140
glyproproleuseraspaspglnileglutyrleuglnasnleu
114511501155
lysserleualaleuthrleuglylysthrasnleualaglnser
116011651170
leuthrthrmetileasnalalysglnserseralaglnargval
117511801185
gluprovalvalvalvalleuargglylysproglycysglylys
119011951200
serleualaserthrleuilealaglnalavalserlysargleu
120512101215
tyrglyserglnservaltyrserleuproproaspproaspphe
122012251230
pheaspglytyrlysglyglnphevalthrleumetaspaspleu
123512401245
glyglnasnproaspglyglnasppheserthrphecysglnmet
125012551260
valserthralaglnpheleuproasnmetalaaspleualaglu
126512701275
lysglyargprophethrserasnleuileilealathrthrasn
128012851290
leuprohispheserprovalthrilealaaspproseralaval
129513001305
serargargileasntyraspleuthrleugluvalsergluala
131013151320
tyrlyslyshisthrargleuasnpheaspleualapheargarg
132513301335
thraspalaproproiletyrprophealaalahisvalprophe
134013451350
valaspvalalavalargphelysasnglyhisglnasnpheasn
135513601365
leuleugluleuvalaspserilecysthraspileargalalys
137013751380
glnglnglyalaargasnmetglnthrleuvalleuglnserpro
138513901395
asngluasnaspaspthrprovalaspglualaleuglyargval
140014051410
leuserproalaalavalaspglualaleuvalaspleuthrpro
141514201425
glualaaspprovalglyargleualaileleualalysleugly
143014351440
leualaleualaalavalthrproglyleuileileleualaval
144514501455
glyleutyrargtyrpheserglyseraspalaaspglngluglu
146014651470
thrglusergluglyservallysalaproargsergluasnala
147514801485
tyraspglyprolyslysasnserlysproproglyalaleuser
149014951500
leumetglumetglnglnproasnvalaspmetglyphegluala
150515101515
alavalalalyslysvalvalvalproilethrphemetvalpro
152015251530
asnargproserglyleuthrglnseralaleuleuvalthrgly
153515401545
argthrpheleuileasngluhisthrtrpserasnprosertrp
155015551560
thrserphethrileargglygluvalhisthrargaspglupro
156515701575
pheglnthrvalhisphethrhishisglyileprothraspleu
158015851590
metmetvalargleuglyproglyasnserpheproasnasnleu
159516001605
asplyspheglyleuaspglnmetproalaargasnserargval
161016151620
valglyvalsersersertyrglyasnphephepheserglyasn
162516301635
pheleuglyphevalaspserilethrsergluglnglythrtyr
164016451650
alaargleupheargtyrargvalthrthrtyrlysglytrpcys
165516601665
glyseralaleuvalcysglualaglyglyvalargargileile
167016751680
glyleuhisseralaglyalaalaglyileglyalaglythrtyr
168516901695
ileserlysleuglyleuilelysalaleulyshisleuglyglu
170017051710
proleualathrmetglnglyleumetthrgluleugluprogly
171517201725
ilethrvalhisvalproarglysserlysleuarglysthrthr
173017351740
alahisalavaltyrlysprogluphegluproalavalleuser
174517501755
lyspheaspproargleuasnlysaspvalaspleuaspgluval
176017651770
iletrpserlyshisthralaasnvalprotyrglnproproleu
177517801785
phetyrthrtyrmetserglutyralahisargvalpheserphe
179017951800
leuglylysaspasnaspileleuthrvallysglualaileleu
180518101815
glyileproglyleuaspprometaspprohisthralaprogly
182018251830
leuprotyralaileasnglyleuargargthraspleuvalasp
183518401845
phevalasnglythrvalaspalaalaleualavalglnilegln
185018551860
lyspheleuaspglyasptyrserasphisvalpheglnthrphe
186518701875
leulysaspgluileargproserglulysvalargalaglylys
188018851890
thrargilevalaspvalproserleualahiscysilevalgly
189519001905
argmetleuleuglyargphealaalalyspheglnserhispro
191019151920
glypheleuleuglyseralaileglyseraspproaspvalphe
192519301935
trpthrvalileglyalaglnleugluglyarglysasnthrtyr
194019451950
aspvalasptyrseralapheaspserserhisglythrglyser
195519601965
pheglualaleuileserhisphephethrvalaspasnglyphe
197019751980
serproalaleuglyprotyrleuargserleualavalserval
198519901995
hisalatyrglygluargargilelysilethrglyglyleupro
200020052010
serglycysalaalathrserleuleuasnthrvalleuasnasn
201520202025
valileileargthralaleualaleuthrtyrlysglupheglu
203020352040
tyraspmetvalaspileilealatyrglyaspaspleuleuval
204520502055
glythrasptyraspleuasppheasngluvalalaargargala
206020652070
alalysleuglytyrlysmetthrproalaasnlysglyserval
207520802085
pheproprothrserserleuseraspalavalpheleulysarg
209020952100
lysphevalglnasnasnaspglyleutyrlysprovalmetasp
210521102115
leulysasnleuglualametleusertyrphelysproglythr
212021252130
leuleuglulysleuglnservalsermetleualaglnhisser
213521402145
glylysgluglutyraspargleumethisprophealaasptyr
215021552160
glyalavalproserhisglutyrleuglnalaargtrpargala
216521702175
leupheasp
2180
<210>16
<211>2181
<212>prt
<213>塞内卡病毒
<400>16
metglnasnserhispheserpheaspthralaserglythrpheglu
151015
aspvalthrglythrlysvallysilevalglutyrproargserval
202530
asnasnglyvaltyraspserserthrhisleugluileleuasnleu
354045
glnglygluilegluileleuargserpheasnglutyrglnilearg
505560
alaalalysglnglnleuglyleuaspilevaltyrgluleuglngly
65707580
asnvalglnthrthrserlysasnasppheaspserargglyasnasn
859095
glyasnmetthrpheasntyrtyralaasnthrtyrglnasnserval
100105110
asppheserthrserserseralaserglyalaglyproglyasnser
115120125
argglyglyleualaglyleuleuthrasnpheserglyileleuasn
130135140
proleuglytyrleulysasphisasnthrgluglumetgluasnser
145150155160
alaaspargvalthrthrglnthralaglyasnthralaileasnthr
165170175
glnserserleuglyvalleucysalatyrvalgluaspprothrlys
180185190
seraspproproserserserthraspglnprothrthrthrphethr
195200205
alaileaspargtrptyrthrglyargleuasnsertrpthrlysala
210215220
vallysthrpheserpheglnalavalproleuproglyalapheleu
225230235240
serargglnglyglyleuasnglyglyalaphethralathrleuhis
245250255
arghispheleumetlyscysglytrpglnvalglnvalglncysasn
260265270
leuthrglnphehisglnglyalaleuleuvalalametvalproglu
275280285
thrthrleuaspvallysproaspglylysalalysserleuglnglu
290295300
leuasnglugluglntrpvalglumetseraspasptyrargthrgly
305310315320
lysasnmetpropheglnserleuglythrtyrtyrargproproasn
325330335
trpthrtrpglyproasnpheileasnprotyrglnvalthrvalphe
340345350
prohisglnileleuasnalaargthrserthrservalaspileasn
355360365
valprotyrileglygluthrprothrglnsersergluthrglnasn
370375380
sertrpthrleuleuvalmetvalleuvalproleuasptyrlysglu
385390395400
glyalathrthraspprogluilethrpheservalargprothrser
405410415
protyrpheasnglyleuargasnargtyrthralaglythraspglu
420425430
gluglnglyproileprothralaproarggluasnserleumetphe
435440445
leuserthrleuproaspaspthrvalproalatyrglyasnvalarg
450455460
thrproprovalasntyrleuproglygluilethraspleuleugln
465470475480
leualaargileprothrleumetalaphegluargvalproglupro
485490495
valproalaseraspthrtyrvalprotyrvalalavalprothrgln
500505510
pheaspaspargproleuileserpheproilethrleuserasppro
515520525
valtyrglnasnthrleuvalglyalaileserserasnphealaasn
530535540
tyrargglycysileglnilethrleuthrphecysglyprometmet
545550555560
alaargglylyspheleuleusertyrserproproasnglythrgln
565570575
proglnthrleuserglualametglncysthrtyrseriletrpasp
580585590
ileglyleuasnsersertrpthrphevalvalprotyrileserpro
595600605
serasptyrarggluthrargalailethrasnservaltyrserala
610615620
aspglytrppheserleuhislysleuthrlysilethrleupropro
625630635640
aspcysproglnserprocysileleuphephealaseralaglyglu
645650655
asptyrthrleuargleuprovalaspcysasnprosertyrvalphe
660665670
hisserthraspasnalagluthrglyvalileglualaglyasnthr
675680685
aspthrasppheserglygluleualaalaproglyserasnhisthr
690695700
asnvallyspheleupheaspargserargleuleuasnvalilelys
705710715720
valleuglulysaspalavalpheproargpropheprothrglnglu
725730735
glyalaglnglnaspaspglytyrphecysleuleuthrproargpro
740745750
thrvalalaserargproalathrargpheglyleutyralaasnpro
755760765
serglyserglyvalleualaasnthrserleuasppheasnphetyr
770775780
serleualacysphethrtyrpheargseraspleugluvalthrval
785790795800
valserleugluproaspleugluphealavalglytrppheproser
805810815
glyserglutyrglnalaserserphevaltyraspglnleuhisval
820825830
prophehisphethrglyargthrproargalaphealaserlysgly
835840845
glylysvalserphevalleuprotrpasnservalserservalleu
850855860
provalargtrpglyglyalaserlysleuserseralathrarggly
865870875880
leuproalahisalaasptrpglythriletyralaphevalproarg
885890895
proasnglulyslysserthralavallyshisvalalavaltyrile
900905910
argtyrlysasnalaargalatrpcysprosermetleuprophearg
915920925
sertyrlysglnlysmetleumetglnserglyaspilegluthrasn
930935940
proglyproalaseraspasnproileleuglupheleuglualaglu
945950955960
asnaspleuvalthrleualaserleutrplysmetvalhisserval
965970975
glnglnthrtrparglystyrvallysasnaspaspphetrpproasn
980985990
leuleusergluleuvalglygluglyservalalaleualaalathr
99510001005
leuserasnglnalaservallysalaleuleuglyleuhisphe
101010151020
leuserargglyleuasntyrthraspphetyrserleuleuile
102510301035
glulyscysserserphephethrvalglupropropropropro
104010451050
alagluasnleumetthrlysproservallysserlysphearg
105510601065
lysleuphelysmetglnglyprometasplysvallysasptrp
107010751080
asnglnilealaalaglyleulysasnpheglnphevalargasp
108510901095
leuvallysgluvalvalasptrpleuglnalatrpileasnlys
110011051110
glulysalaserprovalleuglntyrglnleuglumetlyslys
111511201125
leuglyprovalalaleualahisaspalaphemetalaglyser
113011351140
glyproproleuseraspaspglnileglutyrleuglnasnleu
114511501155
lysserleualaleuthrleuglylysthrasnleualaglnser
116011651170
leuthrthrmetileasnalalysglnserseralaglnargval
117511801185
gluprovalvalvalvalleuargglylysproglycysglylys
119011951200
serleualaserthrleuilealaglnalavalserlysargleu
120512101215
tyrglyserglnservaltyrserleuproproaspproaspphe
122012251230
pheaspglytyrlysglyglnphevalthrleumetaspaspleu
123512401245
glyglnasnproaspglyglnasppheserthrphecysglnmet
125012551260
valserthralaglnpheleuproasnmetalaaspleualaglu
126512701275
lysglyargprophethrserasnleuileilealathrthrasn
128012851290
leuprohispheserprovalthrilealaaspproseralaval
129513001305
serargargileasntyraspleuthrleugluvalsergluala
131013151320
tyrlyslyshisthrargleuasnpheaspleualapheargarg
132513301335
thraspalaproproiletyrprophealaalahisvalprophe
134013451350
valaspvalalavalargphelysasnglyhisglnasnpheasn
135513601365
leuleugluleuvalaspserilecysthraspileargalalys
137013751380
glnglnglyalaargasnmetglnthrleuvalleuglnserpro
138513901395
asngluasnaspaspthrprovalaspglualaleuglyargval
140014051410
leuserproalaalavalaspglualaleuvalaspleuthrpro
141514201425
glualaaspprovalglyargleualaileleualalysleugly
143014351440
leualaleualaalavalthrproglyleuileileleualaval
144514501455
glyleutyrargtyrpheserglyseraspalaaspglngluglu
146014651470
thrglusergluglyservallysalaproargsergluasnala
147514801485
tyraspglyprolyslysasnserlysproproglyalaleuser
149014951500
leumetglumetglnglnproasnvalaspmetglyphegluala
150515101515
alavalalalyslysvalvalvalproilethrphemetvalpro
152015251530
asnargproserglyleuthrglnseralaleuleuvalthrgly
153515401545
argthrpheleuileasngluhisthrtrpserasnprosertrp
155015551560
thrserphethrileargglygluvalhisthrargaspglupro
156515701575
pheglnthrvalhisphethrhishisglyileprothraspleu
158015851590
metmetvalargleuglyproglyasnserpheproasnasnleu
159516001605
asplyspheglyleuaspglnmetproalaargasnserargval
161016151620
valglyvalsersersertyrglyasnphephepheserglyasn
162516301635
pheleuglyphevalaspserilethrsergluglnglythrtyr
164016451650
alaargleupheargtyrargvalthrthrtyrlysglytrpcys
165516601665
glyseralaleuvalcysglualaglyglyvalargargileile
167016751680
glyleuhisseralaglyalaalaglyileglyalaglythrtyr
168516901695
ileserlysleuglyleuilelysalaleulyshisleuglyglu
170017051710
proleualathrmetglnglyleumetthrgluleugluprogly
171517201725
ilethrvalhisvalproarglysserlysleuarglysthrthr
173017351740
alahisalavaltyrlysprogluphegluproalavalleuser
174517501755
lyspheaspproargleuasnlysaspvalaspleuaspgluval
176017651770
iletrpserlyshisthralaasnvalprotyrglnproproleu
177517801785
phetyrthrtyrmetserglutyralahisargvalpheserphe
179017951800
leuglylysaspasnaspileleuthrvallysglualaileleu
180518101815
glyileproglyleuaspprometaspprohisthralaprogly
182018251830
leuprotyralaileasnglyleuargargthraspleuvalasp
183518401845
phevalasnglythrvalaspalaalaleualavalglnilegln
185018551860
lyspheleuaspglyasptyrserasphisvalpheglnthrphe
186518701875
leulysaspgluileargproserglulysvalargalaglylys
188018851890
thrargilevalaspvalproserleualahiscysilevalgly
189519001905
argmetleuleuglyargphealaalalyspheglnserhispro
191019151920
glypheleuleuglyseralaileglyseraspproaspvalphe
192519301935
trpthrvalileglyalaglnleugluglyarglysasnthrtyr
194019451950
aspvalasptyrseralapheaspserserhisglythrglyser
195519601965
pheglualaleuileserhisphephethrvalaspasnglyphe
197019751980
serproalaleuglyprotyrleuargserleualavalserval
198519901995
hisalatyrglygluargargilelysilethrglyglyleupro
200020052010
serglycysalaalathrserleuleuasnthrvalleuasnasn
201520202025
valileileargthralaleualaleuthrtyrlysglupheglu
203020352040
tyraspmetvalaspileilealatyrglyaspaspleuleuval
204520502055
glythrasptyraspleuasppheasngluvalalaargargala
206020652070
alalysleuglytyrlysmetthrproalaasnlysglyserval
207520802085
pheproprothrserserleuseraspalavalpheleulysarg
209020952100
lysphevalglnasnasnaspglyleutyrlysprovalmetasp
210521102115
leulysasnleuglualametleusertyrphelysproglythr
212021252130
leuleuglulysleuglnservalsermetleualaglnhisser
213521402145
glylysgluglutyraspargleumethisprophealaasptyr
215021552160
glyalavalproserhisglutyrleuglnalaargtrpargala
216521702175
leupheasp
2180
<210>17
<211>2181
<212>prt
<213>塞内卡病毒
<400>17
metglnasnserhispheserpheaspthralaserglythrpheglu
151015
aspvalthrglythrlysvallysilevalglutyrproargserval
202530
asnasnglyvaltyraspserserthrhisleugluileleuasnleu
354045
glnglygluilegluileleuargserpheasnglutyrglnilearg
505560
alaalalysglnglnleuglyleuaspilevaltyrgluleuglngly
65707580
asnvalglnthrthrserlysasnasppheaspserargglyasnasn
859095
glyasnmetthrpheasntyrtyralaasnthrtyrglnasnserval
100105110
asppheserthrserserseralaserglyalaglyproglyasnser
115120125
argglyglyleualaglyleuleuthrasnpheserglyileleuasn
130135140
proleuglytyrleulysasphisasnthrgluglumetgluasnser
145150155160
alaaspargvalthrthrglnthralaglyasnthralaileasnthr
165170175
glnserserleuglyvalleucysalatyrvalgluaspprothrlys
180185190
seraspproproserserserthraspglnprothrthrthrphethr
195200205
alaileaspargtrptyrthrglyargleuasnsertrpthrlysala
210215220
vallysthrpheserpheglnalavalproleuproglyalapheleu
225230235240
serargglnglyglyleuasnglyglyalaphethralathrleuhis
245250255
arghispheleumetlyscysglytrpglnvalglnvalglncysasn
260265270
leuthrglnphehisglnglyalaleuleuvalalametvalproglu
275280285
thrthrleuaspvallysproaspglylysalalysserleuglnglu
290295300
leuasnglugluglntrpvalglumetseraspasptyrargthrgly
305310315320
lysasnmetpropheglnserleuglythrtyrtyrargproproasn
325330335
trpthrtrpglyproasnpheileasnprotyrglnvalthrvalphe
340345350
prohisglnileleuasnalaargthrserthrservalaspileasn
355360365
valprotyrileglygluthrprothrglnsersergluthrglnasn
370375380
sertrpthrleuleuvalmetvalleuvalproleuasptyrlysglu
385390395400
glyalathrthraspprogluilethrpheservalargprothrser
405410415
protyrpheasnglyleuargasnargtyrthralaglythraspglu
420425430
gluglnglyproileprothralaproarggluasnserleumetphe
435440445
leuserthrleuproaspaspthrvalproalatyrglyasnvalarg
450455460
thrproprovalasntyrleuproglygluilethraspleuleugln
465470475480
leualaargileprothrleumetalaphegluargvalproglupro
485490495
valproalaseraspthrtyrvalprotyrvalalavalprothrgln
500505510
pheaspaspargproleuileserpheproilethrleuserasppro
515520525
valtyrglnasnthrleuvalglyalaileserserasnphealaasn
530535540
tyrargglycysileglnilethrleuthrphecysglyprometmet
545550555560
alaargglylyspheleuleusertyrserproproasnglythrgln
565570575
proglnthrleuserglualametglncysthrtyrseriletrpasp
580585590
ileglyleuasnsersertrpthrphevalvalprotyrileserpro
595600605
serasptyrarggluthrargalailethrasnservaltyrserala
610615620
aspglytrppheserleuhislysleuthrlysilethrleupropro
625630635640
aspcysproglnserprocysileleuphephealaseralaglyglu
645650655
asptyrthrleuargleuprovalaspcysasnprosertyrvalphe
660665670
hisserthraspasnalagluthrglyvalileglualaglyasnthr
675680685
aspthrasppheserglygluleualaalaproglyserasnhisthr
690695700
asnvallyspheleupheaspargserargleuleuasnvalilelys
705710715720
valleuglulysaspalavalpheproargpropheprothrglnglu
725730735
glyalaglnglnaspaspglytyrphecysleuleuthrproargpro
740745750
thrvalalaserargproalathrargpheglyleutyralaasnpro
755760765
serglyserglyvalleualaasnthrserleuasppheasnphetyr
770775780
serleualacysphethrtyrpheargseraspleugluvalthrval
785790795800
valserleugluproaspleugluphealavalglytrppheproser
805810815
glyserglutyrglnalaserserphevaltyraspglnleuhisval
820825830
prophehisphethrglyargthrproargalaphealaserlysgly
835840845
glylysvalserphevalleuprotrpasnservalserservalleu
850855860
provalargtrpglyglyalaserlysleuserseralathrarggly
865870875880
leuproalahisalaasptrpglythriletyralaphevalproarg
885890895
proasnglulyslysserthralavallyshisvalalavaltyrile
900905910
argtyrlysasnalaargalatrpcysprosermetleuprophearg
915920925
sertyrlysglnlysmetleumetglnserglyaspilegluthrasn
930935940
proglyproalaseraspasnproileleuglupheleuglualaglu
945950955960
asnaspleuvalthrleualaserleutrplysmetvalhisserval
965970975
glnglnthrtrparglystyrvallysasnaspaspphetrpproasn
980985990
leuleusergluleuvalglygluglyservalalaleualaalathr
99510001005
leuserasnglnalaservallysalaleuleuglyleuhisphe
101010151020
leuserargglyleuasntyrthraspphetyrserleuleuile
102510301035
glulyscysserserphephethrvalglupropropropropro
104010451050
alagluasnleumetthrlysproservallysserlysphearg
105510601065
lysleuphelysmetglnglyprometasplysvallysasptrp
107010751080
asnglnilealaalaglyleulysasnpheglnphevalargasp
108510901095
leuvallysgluvalvalasptrpleuglnalatrpileasnlys
110011051110
glulysalaserprovalleuglntyrglnleuglumetlyslys
111511201125
leuglyprovalalaleualahisaspalaphemetalaglyser
113011351140
glyproproleuseraspaspglnileglutyrleuglnasnleu
114511501155
lysserleualaleuthrleuglylysthrasnleualaglnser
116011651170
leuthrthrmetileasnalalysglnserseralaglnargval
117511801185
gluprovalvalvalvalleuargglylysproglycysglylys
119011951200
serleualaserthrleuilealaglnalavalserlysargleu
120512101215
tyrglyserglnservaltyrserleuproproaspproaspphe
122012251230
pheaspglytyrlysglyglnphevalthrleumetaspaspleu
123512401245
glyglnasnproaspglyglnasppheserthrphecysglnmet
125012551260
valserthralaglnpheleuproasnmetalaaspleualaglu
126512701275
lysglyargprophethrserasnleuileilealathrthrasn
128012851290
leuprohispheserprovalthrilealaaspproseralaval
129513001305
serargargileasntyraspleuthrleugluvalsergluala
131013151320
tyrlyslyshisthrargleuasnpheaspleualapheargarg
132513301335
thraspalaproproiletyrprophealaalahisvalprophe
134013451350
valaspvalalavalargphelysasnglyhisglnasnpheasn
135513601365
leuleugluleuvalaspserilecysthraspileargalalys
137013751380
glnglnglyalaargasnmetglnthrleuvalleuglnserpro
138513901395
asngluasnaspaspthrprovalaspglualaleuglyargval
140014051410
leuserproalaalavalaspglualaleuvalaspleuthrpro
141514201425
glualaaspprovalglyargleualaileleualalysleugly
143014351440
leualaleualaalavalthrproglyleuileileleualaval
144514501455
glyleutyrargtyrpheserglyseraspalaaspglngluglu
146014651470
thrglusergluglyservallysalaproargsergluasnala
147514801485
tyraspglyprolyslysasnserlysproproglyalaleuser
149014951500
leumetglumetglnglnproasnvalaspmetglyphegluala
150515101515
alavalalalyslysvalvalvalproilethrphemetvalpro
152015251530
asnargproserglyleuthrglnseralaleuleuvalthrgly
153515401545
argthrpheleuileasngluhisthrtrpserasnprosertrp
155015551560
thrserphethrileargglygluvalhisthrargaspglupro
156515701575
pheglnthrvalhisphethrhishisglyileprothraspleu
158015851590
metmetvalargleuglyproglyasnserpheproasnasnleu
159516001605
asplyspheglyleuaspglnmetproalaargasnserargval
161016151620
valglyvalsersersertyrglyasnphephepheserglyasn
162516301635
pheleuglyphevalaspserilethrsergluglnglythrtyr
164016451650
alaargleupheargtyrargvalthrthrtyrlysglytrpcys
165516601665
glyseralaleuvalcysglualaglyglyvalargargileile
167016751680
glyleuhisseralaglyalaalaglyileglyalaglythrtyr
168516901695
ileserlysleuglyleuilelysalaleulyshisleuglyglu
170017051710
proleualathrmetglnglyleumetthrgluleugluprogly
171517201725
ilethrvalhisvalproarglysserlysleuarglysthrthr
173017351740
alahisalavaltyrlysprogluphegluproalavalleuser
174517501755
lyspheaspproargleuasnlysaspvalaspleuaspgluval
176017651770
iletrpserlyshisthralaasnvalprotyrglnproproleu
177517801785
phetyrthrtyrmetserglutyralahisargvalpheserphe
179017951800
leuglylysaspasnaspileleuthrvallysglualaileleu
180518101815
glyileproglyleuaspprometaspprohisthralaprogly
182018251830
leuprotyralaileasnglyleuargargthraspleuvalasp
183518401845
phevalasnglythrvalaspalaalaleualavalglnilegln
185018551860
lyspheleuaspglyasptyrserasphisvalpheglnthrphe
186518701875
leulysaspgluileargproserglulysvalargalaglylys
188018851890
thrargilevalaspvalproserleualahiscysilevalgly
189519001905
argmetleuleuglyargphealaalalyspheglnserhispro
191019151920
glypheleuleuglyseralaileglyseraspproaspvalphe
192519301935
trpthrvalileglyalaglnleugluglyarglysasnthrtyr
194019451950
aspvalasptyrseralapheaspserserhisglythrglyser
195519601965
pheglualaleuileserhisphephethrvalaspasnglyphe
197019751980
serproalaleuglyprotyrleuargserleualavalserval
198519901995
hisalatyrglygluargargilelysilethrglyglyleupro
200020052010
serglycysalaalathrserleuleuasnthrvalleuasnasn
201520202025
valileileargthralaleualaleuthrtyrlysglupheglu
203020352040
tyraspmetvalaspileilealatyrglyaspaspleuleuval
204520502055
glythrasptyraspleuasppheasngluvalalaargargala
206020652070
alalysleuglytyrlysmetthrproalaasnlysglyserval
207520802085
pheproprothrserserleuseraspalavalpheleulysarg
209020952100
lysphevalglnasnasnaspglyleutyrlysprovalmetasp
210521102115
leulysasnleuglualametleusertyrphelysproglythr
212021252130
leuleuglulysleuglnservalsermetleualaglnhisser
213521402145
glylysgluglutyraspargleumethisprophealaasptyr
215021552160
glyalavalproserhisglutyrleuglnalaargtrpargala
216521702175
leupheasp
2180
<210>18
<211>3308
<212>dna
<213>塞内卡病毒
<400>18
ggatccgccaccatgggtaatgttcagacaacctcaaagaatgattttgattcccgcggc60
aataatggtaacatgaccttcaattactacgcaaacacttaccagaattcagtagacttc120
tcgacctcctcgtcggcgtcaggcgccggacccgggaactcccggggcggactagcgggt180
ctcctcacaaatttcagtggaatcttgaaccctcttggctacctcaaagatcacaatacc240
gaagaaatggaaaactctgctgatcgagtcataacgcaaacggcgggcaacactgccata300
aacacgcaatcatcactgggtgtgttgtgtgcctacgttgaagacccgaccaaatctgac360
cctccgtccagcagcacagatcaacccaccaccacttttactgccatcgacaggtggtac420
actggacgcctcaattcttggacaaaagctgtaaaaaccttctcttttcaggccgtcccg480
ctccctggagccttcctgtctaaacagggaggcctcaacggaggggccttcacggctacc540
ctacatagacatttcttaatgaagtgcgggtggcaggtgcaggtccaatgcaatttgacg600
caattccaccaaggtgctcttcttgttgccatggtccccgaaaccacccttgatgtcaaa660
cctgacggcaaggcaaagagcttacaggagctgaatgaagagcagtgggtggaaatgtct720
gacgattaccggaccgggaaaaacatgccttttcagtctcttggcacatactatcggccc780
cctaactggacttggggccccaatttcatcaacccctatcaagtaacagttttcccacac840
caaattctgaacgcgagaacctctacctcggtagacataagtgtcccatacatcggggag900
actcctacacaatcctcagagacacagaactcctggaccctccttgttatggtgcttgtc960
cccctggactacaaggagggagccacaactgacccagaaattacattttctgtaaggcct1020
acaagtccctacttcaatgggcttcgtaaccgtttcacgaccgggacggacgaggaacag1080
gggcccattcccacagcacccagagaaaattcgcttatgtttctctcaaccatccctgac1140
gacactgtccctgcttacgggaatgtgcgtacccctcccgtcaattacctccccggtgaa1200
ataaccgacctcttacaactggcccgtatacccactctcatggcgtttgggcgggtgtct1260
gaacccgagcctgcctcagacgcatatgtgccctacgttgccgttcctgcccagttcgac1320
gacaagcctctcatctccttcccgatcaccctttcagatcctgtctaccagaacaccctg1380
gtaggcgccatcagttcgaacttcgccaactaccgggggtgtatccaaatcactctgaca1440
ttttgtggacccatgatggcaagagggaaattcctgctctcgtattctcccccaaatgga1500
gcacaaccacagaccctttctgaagctatgcagtgcacatactctatttgggatataggc1560
ttgaactctagttggacctttgtcatcccctacatctcgcccagtgattaccgtgaaact1620
cgggctattaccaactcagtttattctgctgatggttggtttagcttgcacaagctgacc1680
aaaattactctaccacctgactgcccacagagtccctgtattctctttttcgcctctgct1740
ggtgaggattacaccctccgtctccctgttgattgtaatccttcctacgtgttccactcc1800
accgacaacgccgagactggggttattgaggcaggtaacactgacaccgatttctctggt1860
gaactggcggctcctggctctaaccatactaatgtcaaattcctgtttgaccgatctcga1920
ctactgaatgtaattaaggtactggagaaggacgccgtcttcccccgtcctttccccaca1980
gcaacaggtgcacagcaggacgatggttacttttgtcttctaacaccccgcccaacagtc2040
gcttcccgacccgccactcgtttcggcctgtacgtcaacccgtctgacagtggcgttctc2100
gctaacacttcactggatttcaatttttacagtttggcctgtttcacttactttagatca2160
gaccttgaagtcacggtggtctcactggagccagatttggaattcgccgtggggtggttc2220
ccctctggcagtgagtaccaggcttctagctttgtctacgaccaactgcatgtaccctac2280
cactttactgggcgcactccccgcgctttcaccagcaagggtggaaaggtatctttcgtg2340
ctcccttggaactctgtctcttccgtgcttcccgtgcgctgggggggcgcttccaagctt2400
tcttctgccacgcggggtctgccggctcatgctgactgggggaccatttacgcctttatc2460
ccccgtcctaacgagaagaaaagcaccgctgtaaagcacgtggcggtgtacgttcggtac2520
aagaacgcgcgtgcttggtgccccagcatgcttccctttcgcagctacaagcaaaagatg2580
ctgatgcaatcaggcgacatcgagaccaaccctggccctggttcaggtaagccccctgga2640
gcgctctctcttatggaaatgcaacagcccaacgtggacatgggctttgaggctgcggtc2700
gctaagaaagtggtcgtccccattaccttcatggttcccaacagaccttctggacttaca2760
cagtccgctcttcttgtggccggccggaccttcctaatcaatgagcatacatggtccaac2820
ccctcctggaccagcttcacaatccgtggtgaggtgcacactcgtgatgagcctttccaa2880
acggttcattttactcaccatggtcttcccacagatctgatgatggtacgtctcggaccg2940
ggcaactctttccctaacaatctagacaagtttggacttgaccagatgccggcacgtaac3000
tcccgtgtggttggcgtttcggctagttacggtaacttcttcttctctgggaacttcctc3060
gggtttgttgactccatcacctctgaccaaggaacctatgcgagacttttcaggtacagg3120
gtgacgacttacaagggatggtgcggttcggccctggtctgtgaggccggtggtgtccga3180
cgcatcattggcatgcattctgctggtgccgctggtatcggcgccgggacttacatctca3240
aaattaggactgatcaaagcccttaaacacctcggtgagcctctggctacaatgcaatga3300
gcggccgc3308
<210>19
<211>1095
<212>prt
<213>塞内卡病毒
<400>19
metglyasnvalglnthrthrserlysasnasppheaspserarggly
151015
asnasnglyasnmetthrpheasntyrtyralaasnthrtyrglnasn
202530
servalasppheserthrserserseralaserglyalaglyprogly
354045
asnserargglyglyleualaglyleuleuthrasnpheserglyile
505560
leuasnproleuglytyrleulysasphisasnthrgluglumetglu
65707580
asnseralaaspargvalilethrglnthralaglyasnthralaile
859095
asnthrglnserserleuglyvalleucysalatyrvalgluasppro
100105110
thrlysseraspproproserserserthraspglnprothrthrthr
115120125
phethralaileaspargtrptyrthrglyargleuasnsertrpthr
130135140
lysalavallysthrpheserpheglnalavalproleuproglyala
145150155160
pheleuserlysglnglyglyleuasnglyglyalaphethralathr
165170175
leuhisarghispheleumetlyscysglytrpglnvalglnvalgln
180185190
cysasnleuthrglnphehisglnglyalaleuleuvalalametval
195200205
progluthrthrleuaspvallysproaspglylysalalysserleu
210215220
glngluleuasnglugluglntrpvalglumetseraspasptyrarg
225230235240
thrglylysasnmetpropheglnserleuglythrtyrtyrargpro
245250255
proasntrpthrtrpglyproasnpheileasnprotyrglnvalthr
260265270
valpheprohisglnileleuasnalaargthrserthrservalasp
275280285
ileservalprotyrileglygluthrprothrglnsersergluthr
290295300
glnasnsertrpthrleuleuvalmetvalleuvalproleuasptyr
305310315320
lysgluglyalathrthraspprogluilethrpheservalargpro
325330335
thrserprotyrpheasnglyleuargasnargphethrthrglythr
340345350
aspglugluglnglyproileprothralaproarggluasnserleu
355360365
metpheleuserthrileproaspaspthrvalproalatyrglyasn
370375380
valargthrproprovalasntyrleuproglygluilethraspleu
385390395400
leuglnleualaargileprothrleumetalapheglyargvalser
405410415
gluprogluproalaseraspalatyrvalprotyrvalalavalpro
420425430
alaglnpheaspasplysproleuileserpheproilethrleuser
435440445
aspprovaltyrglnasnthrleuvalglyalaileserserasnphe
450455460
alaasntyrargglycysileglnilethrleuthrphecysglypro
465470475480
metmetalaargglylyspheleuleusertyrserproproasngly
485490495
alaglnproglnthrleuserglualametglncysthrtyrserile
500505510
trpaspileglyleuasnsersertrpthrphevalileprotyrile
515520525
serproserasptyrarggluthrargalailethrasnservaltyr
530535540
seralaaspglytrppheserleuhislysleuthrlysilethrleu
545550555560
proproaspcysproglnserprocysileleuphephealaserala
565570575
glygluasptyrthrleuargleuprovalaspcysasnprosertyr
580585590
valphehisserthraspasnalagluthrglyvalileglualagly
595600605
asnthraspthrasppheserglygluleualaalaproglyserasn
610615620
histhrasnvallyspheleupheaspargserargleuleuasnval
625630635640
ilelysvalleuglulysaspalavalpheproargpropheprothr
645650655
alathrglyalaglnglnaspaspglytyrphecysleuleuthrpro
660665670
argprothrvalalaserargproalathrargpheglyleutyrval
675680685
asnproseraspserglyvalleualaasnthrserleuasppheasn
690695700
phetyrserleualacysphethrtyrpheargseraspleugluval
705710715720
thrvalvalserleugluproaspleugluphealavalglytrpphe
725730735
proserglyserglutyrglnalaserserphevaltyraspglnleu
740745750
hisvalprotyrhisphethrglyargthrproargalaphethrser
755760765
lysglyglylysvalserphevalleuprotrpasnservalserser
770775780
valleuprovalargtrpglyglyalaserlysleuserseralathr
785790795800
argglyleuproalahisalaasptrpglythriletyralapheile
805810815
proargproasnglulyslysserthralavallyshisvalalaval
820825830
tyrvalargtyrlysasnalaargalatrpcysprosermetleupro
835840845
pheargsertyrlysglnlysmetleumetglnserglyaspileglu
850855860
thrasnproglyproglyserglylysproproglyalaleuserleu
865870875880
metglumetglnglnproasnvalaspmetglypheglualaalaval
885890895
alalyslysvalvalvalproilethrphemetvalproasnargpro
900905910
serglyleuthrglnseralaleuleuvalalaglyargthrpheleu
915920925
ileasngluhisthrtrpserasnprosertrpthrserphethrile
930935940
argglygluvalhisthrargaspglupropheglnthrvalhisphe
945950955960
thrhishisglyleuprothraspleumetmetvalargleuglypro
965970975
glyasnserpheproasnasnleuasplyspheglyleuaspglnmet
980985990
proalaargasnserargvalvalglyvalseralasertyrglyasn
99510001005
phephepheserglyasnpheleuglyphevalaspserilethr
101010151020
seraspglnglythrtyralaargleupheargtyrargvalthr
102510301035
thrtyrlysglytrpcysglyseralaleuvalcysglualagly
104010451050
glyvalargargileileglymethisseralaglyalaalagly
105510601065
ileglyalaglythrtyrileserlysleuglyleuilelysala
107010751080
leulyshisleuglygluproleualathrmetgln
108510901095
<210>20
<211>3308
<212>dna
<213>塞内卡病毒
<400>20
ggatccgccaccatgggcaacgttcaaactacctccaagaatgattttgactcccgcggc60
aacaacggcaacatgacttttaactactacgccaatacgtatcaaaactctgttgatttc120
tcgacatcgagttccgcttccggcgcgggacctggaaattcccgcggtggactggccggt180
ctgctcacaaacttttccggtatcctgaaccccctcggttatctgaaagatcacaatacg240
gaagagatggagaactctgccgatcgtgtgattacgcagacagctggtaacacggctatc300
aacactcaatcgtctctgggagtactctgtgcttacgtcgaggacccaacaaaatccgac360
cctccatcttcctcgaccgatcaacctacgactaccttcacggccatagacaggtggtat420
acaggtcgcctgaattcgtggacaaaggctgttaaaacctttagcttccaggctgtgcca480
ttgcccggtgctttcctttcccgccagggcggcctcaacggtggagcttttactgctaca540
ctgcacagacactttctgatgaaatgcggttggcaggtgcaggtacagtgcaatttgact600
caattccaccaaggtgcacttctcgttgctatggttcccgagactacgcttgacgttaag660
cctgacggcaaggccaagtctctccaagagctgaatgaggaacagtgggttgagatgagc720
gatgactatagaaccggtaaaaacatgccattccagtccctgggcacatactacagaccc780
ccaaattggacttggggcccgaactttatcaatccgtatcaggtcaccgtgtttccacac840
caaatcctgaatgccaggactagtaccagcgtcgatattagcgtgccctatatcggcgaa900
acgccaacccaatcctccgaaactcagaacagctggacgcttctggtaatggtgttggta960
cccttggattataaggagggagccactaccgaccctgaaatcactttttcggtaaggccc1020
actagcccttacttcaacggactgcgcaaccgtttcaccaccggcactgatgaggagcaa1080
ggccctatccctactgcccccagagaaaatagcctgatgtttctgagcacaattcctgac1140
gacaccgtacctgcctatggtaacgttcgtacgcctcccgtaaactacctgcctggtgaa1200
attacggacctcttgcagctggcccgcatccctacactgatggccttcggtcgtgtcagt1260
gagcccgaaccagccagcgacgcttacgttccatatgttgcggtacctgctcagttcgac1320
gacaagccgttgataagtttcccaatcactttgagcgaccctgtataccagaacactttg1380
gttggagctatctcctcgaactttgcgaactaccgtggttgtattcaaatcacccttaca1440
ttctgcggcccgatgatggctcgtggcaagttcctgttgagctactccccccccaacgga1500
gcacaaccacagacactgtccgaggccatgcagtgcacatactccatctgggatatcggt1560
ctcaacagctcatggacctttgtaataccatacatctcgccatccgattatagagagacc1620
cgcgccatcaccaacagtgtgtactcagccgatggatggttcagtctccataagcttaca1680
aagatcactctgccccccgactgcccacaatcaccctgcatattgttctttgcctcagct1740
ggtgaagattacacgcttcgtttgcccgtcgactgtaacccgtcttacgtgttccactcc1800
acggacaatgctgagaccggtgtaattgaggctggtaacaccgacacggatttctctggt1860
gagctggcggctcccggttctaaccataccaacgtgaagttccttttcgaccgttcacgt1920
cttttgaacgtaatcaaggtcttggaaaaggacgctgtgttccctcgcccattccccaca1980
gcaacgggagcacaacaagacgacggctatttctgcctgctgacaccacgtcctactgtg2040
gctagccgtccggccacacgctttggtctctacgttaaccctagcgactctggtgtactg2100
gctaacacctccctggacttcaatttctactctctggcctgcttcacgtacttcaggagc2160
gacctggaggtcactgtggtgtctctggaaccagatctggaatttgcggtaggatggttc2220
ccttcaggaagcgagtaccaggcatcgtcctttgtgtacgaccaacttcatgttccctac2280
catttcaccggtagaaccccacgtgccttcaccagtaagggaggcaaagtttcttttgta2340
ctgccctggaattctgtcagttccgttctcccggtccgctggggcggagcgtcaaagctc2400
tcatccgcgacccgcggattgcccgcacacgcggattggggaactatctacgctttcatt2460
ccacgccctaatgaaaagaagtctacagcagtgaagcacgtggccgtctatgtgcgttac2520
aagaatgcaagggcgtggtgtccgtctatgttgccattccgtagctataaacagaagatg2580
cttatgcaaagcggtgatattgagacgaatcctggacctggttcaggtaagccccctgga2640
gcgctctctcttatggaaatgcaacagcccaacgtggacatgggctttgaggctgcggtc2700
gctaagaaagtggtcgtccccattaccttcatggttcccaacagaccttctggacttaca2760
cagtccgctcttcttgtggccggccggaccttcctaatcaatgagcatacatggtccaac2820
ccctcctggaccagcttcacaatccgtggtgaggtgcacactcgtgatgagcctttccaa2880
acggttcattttactcaccatggtcttcccacagatctgatgatggtacgtctcggaccg2940
ggcaactctttccctaacaatctagacaagtttggacttgaccagatgccggcacgtaac3000
tcccgtgtggttggcgtttcggctagttacggtaacttcttcttctctgggaacttcctc3060
gggtttgttgactccatcacctctgaccaaggaacctatgcgagacttttcaggtacagg3120
gtgacgacttacaagggatggtgcggttcggccctggtctgtgaggccggtggtgtccga3180
cgcatcattggcatgcattctgctggtgccgctggtatcggcgccgggacttacatctca3240
aaattaggactgatcaaagcccttaaacacctcggtgagcctctggctacaatgcaatga3300
gcggccgc3308
<210>21
<211>1095
<212>prt
<213>塞内卡病毒
<400>21
metglyasnvalglnthrthrserlysasnasppheaspserarggly
151015
asnasnglyasnmetthrpheasntyrtyralaasnthrtyrglnasn
202530
servalasppheserthrserserseralaserglyalaglyprogly
354045
asnserargglyglyleualaglyleuleuthrasnpheserglyile
505560
leuasnproleuglytyrleulysasphisasnthrgluglumetglu
65707580
asnseralaaspargvalilethrglnthralaglyasnthralaile
859095
asnthrglnserserleuglyvalleucysalatyrvalgluasppro
100105110
thrlysseraspproproserserserthraspglnprothrthrthr
115120125
phethralaileaspargtrptyrthrglyargleuasnsertrpthr
130135140
lysalavallysthrpheserpheglnalavalproleuproglyala
145150155160
pheleuserargglnglyglyleuasnglyglyalaphethralathr
165170175
leuhisarghispheleumetlyscysglytrpglnvalglnvalgln
180185190
cysasnleuthrglnphehisglnglyalaleuleuvalalametval
195200205
progluthrthrleuaspvallysproaspglylysalalysserleu
210215220
glngluleuasnglugluglntrpvalglumetseraspasptyrarg
225230235240
thrglylysasnmetpropheglnserleuglythrtyrtyrargpro
245250255
proasntrpthrtrpglyproasnpheileasnprotyrglnvalthr
260265270
valpheprohisglnileleuasnalaargthrserthrservalasp
275280285
ileservalprotyrileglygluthrprothrglnsersergluthr
290295300
glnasnsertrpthrleuleuvalmetvalleuvalproleuasptyr
305310315320
lysgluglyalathrthraspprogluilethrpheservalargpro
325330335
thrserprotyrpheasnglyleuargasnargphethrthrglythr
340345350
aspglugluglnglyproileprothralaproarggluasnserleu
355360365
metpheleuserthrileproaspaspthrvalproalatyrglyasn
370375380
valargthrproprovalasntyrleuproglygluilethraspleu
385390395400
leuglnleualaargileprothrleumetalapheglyargvalser
405410415
gluprogluproalaseraspalatyrvalprotyrvalalavalpro
420425430
alaglnpheaspasplysproleuileserpheproilethrleuser
435440445
aspprovaltyrglnasnthrleuvalglyalaileserserasnphe
450455460
alaasntyrargglycysileglnilethrleuthrphecysglypro
465470475480
metmetalaargglylyspheleuleusertyrserproproasngly
485490495
alaglnproglnthrleuserglualametglncysthrtyrserile
500505510
trpaspileglyleuasnsersertrpthrphevalileprotyrile
515520525
serproserasptyrarggluthrargalailethrasnservaltyr
530535540
seralaaspglytrppheserleuhislysleuthrlysilethrleu
545550555560
proproaspcysproglnserprocysileleuphephealaserala
565570575
glygluasptyrthrleuargleuprovalaspcysasnprosertyr
580585590
valphehisserthraspasnalagluthrglyvalileglualagly
595600605
asnthraspthrasppheserglygluleualaalaproglyserasn
610615620
histhrasnvallyspheleupheaspargserargleuleuasnval
625630635640
ilelysvalleuglulysaspalavalpheproargpropheprothr
645650655
alathrglyalaglnglnaspaspglytyrphecysleuleuthrpro
660665670
argprothrvalalaserargproalathrargpheglyleutyrval
675680685
asnproseraspserglyvalleualaasnthrserleuasppheasn
690695700
phetyrserleualacysphethrtyrpheargseraspleugluval
705710715720
thrvalvalserleugluproaspleugluphealavalglytrpphe
725730735
proserglyserglutyrglnalaserserphevaltyraspglnleu
740745750
hisvalprotyrhisphethrglyargthrproargalaphethrser
755760765
lysglyglylysvalserphevalleuprotrpasnservalserser
770775780
valleuprovalargtrpglyglyalaserlysleuserseralathr
785790795800
argglyleuproalahisalaasptrpglythriletyralapheile
805810815
proargproasnglulyslysserthralavallyshisvalalaval
820825830
tyrvalargtyrlysasnalaargalatrpcysprosermetleupro
835840845
pheargsertyrlysglnlysmetleumetglnserglyaspileglu
850855860
thrasnproglyproglyserglylysproproglyalaleuserleu
865870875880
metglumetglnglnproasnvalaspmetglypheglualaalaval
885890895
alalyslysvalvalvalproilethrphemetvalproasnargpro
900905910
serglyleuthrglnseralaleuleuvalalaglyargthrpheleu
915920925
ileasngluhisthrtrpserasnprosertrpthrserphethrile
930935940
argglygluvalhisthrargaspglupropheglnthrvalhisphe
945950955960
thrhishisglyleuprothraspleumetmetvalargleuglypro
965970975
glyasnserpheproasnasnleuasplyspheglyleuaspglnmet
980985990
proalaargasnserargvalvalglyvalseralasertyrglyasn
99510001005
phephepheserglyasnpheleuglyphevalaspserilethr
101010151020
seraspglnglythrtyralaargleupheargtyrargvalthr
102510301035
thrtyrlysglytrpcysglyseralaleuvalcysglualagly
104010451050
glyvalargargileileglymethisseralaglyalaalagly
105510601065
ileglyalaglythrtyrileserlysleuglyleuilelysala
107010751080
leulyshisleuglygluproleualathrmetgln
108510901095
<210>22
<211>3308
<212>dna
<213>塞内卡病毒
<400>22
ggatccgccaccatgggtaatgttcagacaacctcaaagaatgattttgattcccgcggc60
aataatggtaacatgaccttcaattactacgcaaacacttaccagaattcagtagacttc120
tcgacctcctcgtcggcgtcaggcgccggacccgggaactcccggggcggactagcgggt180
ctcctcacaaatttcagtggaatcttgaaccctcttggctacctcaaagatcacaatacc240
gaagaaatggaaaactctgctgatcgagtcataacgcaaacggcgggcaacactgccata300
aacacgcaatcatcactgggtgtgttgtgtgcctacgttgaagacccgaccaaatctgac360
cctccgtccagcagcacagatcaacccaccaccacttttactgccatcgacaggtggtac420
actggacgcctcaattcttggacaaaagctgtaaaaaccttctcttttcaggccgtcccg480
ctccctggagccttcctgtctaaacagggaggcctcaacggaggggccttcacggctacc540
ctacatagacatttcttaatgaagtgcgggtggcaggtgcaggtccaatgcaatttgacg600
caattccaccaaggtgctcttcttgttgccatggtccccgaaaccacccttgatgtcaaa660
cctgacggcaaggcaaagagcttacaggagctgaatgaagagcagtgggtggaaatgtct720
gacgattaccggaccgggaaaaacatgccttttcagtctcttggcacatactatcggccc780
cctaactggacttggggccccaatttcatcaacccctatcaagtaacagttttcccacac840
caaattctgaacgcgagaacctctacctcggtagacataagtgtcccatacatcggggag900
actcctacacaatcctcagagacacagaactcctggaccctccttgttatggtgcttgtc960
cccctggactacaaggagggagccacaactgacccagaaattacattttctgtaaggcct1020
acaagtccctacttcaatgggcttcgtaaccgtttcacgaccgggacggacgaggaacag1080
gggcccattcccacagcacccagagaaaattcgcttatgtttctctcaaccatccctgac1140
gacactgtccctgcttacgggaatgtgcgtacccctcccgtcaattacctccccggtgaa1200
ataaccgacctcttacaactggcccgtatacccactctcatggcgtttgggcgggtgtct1260
gaacccgagcctgcctcagacgcatatgtgccctacgttgccgttcctgcccagttcgac1320
gacaagcctctcatctccttcccgatcaccctttcagatcctgtctaccagaacaccctg1380
gtaggcgccatcagttcgaacttcgccaactaccgggggtgtatccaaatcactctgaca1440
ttttgtggacccatgatggcaagagggaaattcctgctctcgtattctcccccaaatgga1500
gcacaaccacagaccctttctgaagctatgcagtgcacatactctatttgggatataggc1560
ttgaactctagttggacctttgtcatcccctacatctcgcccagtgattaccgtgaaact1620
cgggctattaccaactcagtttattctgctgatggttggtttagcttgcacaagctgacc1680
aaaattactctaccacctgactgcccacagagtccctgtattctctttttcgcctctgct1740
ggtgaggattacaccctccgtctccctgttgattgtaatccttcctacgtgcctcagggg1800
gttgacaacgccgagactggggttattgaggcaggtaacactgacaccgatttctctggt1860
gaactggcggctcctggctctaaccatactaatgtcaaattcctgtttgaccgatctcga1920
ctactgaatgtaattaaggtactggagaaggacgccgtcttcccccgtcctttccccaca1980
gcaacaggtgcacagcaggacgatggttacttttgtcttctaacaccccgcccaacagtc2040
gcttcccgacccgccactcgtttcggcctgtacgtcaacccgtctgacagtggcgttctc2100
gctaacacttcactggatttcaatttttacagtttggcctgtttcacttactttagatca2160
gaccttgaagtcacggtggtctcactggagccagatttggaattcgccgtggggtggttc2220
ccctctggcagtgagtaccaggcttctagctttgtctacgaccaactgcatgtaccctac2280
cactttactgggcgcactccccgcgctttcaccagcaagggtggaaaggtatctttcgtg2340
ctcccttggaactctgtctcttccgtgcttcccgtgcgctgggggggcgcttccaagctt2400
tcttctgccacgcggggtctgccggctcatgctgactgggggaccatttacgcctttatc2460
ccccgtcctaacgagaagaaaagcaccgctgtaaagcacgtggcggtgtacgttcggtac2520
aagaacgcgcgtgcttggtgccccagcatgcttccctttcgcagctacaagcaaaagatg2580
ctgatgcaatcaggcgacatcgagaccaaccctggccctggttcaggtaagccccctgga2640
gcgctctctcttatggaaatgcaacagcccaacgtggacatgggctttgaggctgcggtc2700
gctaagaaagtggtcgtccccattaccttcatggttcccaacagaccttctggacttaca2760
cagtccgctcttcttgtggccggccggaccttcctaatcaatgagcatacatggtccaac2820
ccctcctggaccagcttcacaatccgtggtgaggtgcacactcgtgatgagcctttccaa2880
acggttcattttactcaccatggtcttcccacagatctgatgatggtacgtctcggaccg2940
ggcaactctttccctaacaatctagacaagtttggacttgaccagatgccggcacgtaac3000
tcccgtgtggttggcgtttcggctagttacggtaacttcttcttctctgggaacttcctc3060
gggtttgttgactccatcacctctgaccaaggaacctatgcgagacttttcaggtacagg3120
gtgacgacttacaagggatggtgcggttcggccctggtctgtgaggccggtggtgtccga3180
cgcatcattggcatgcattctgctggtgccgctggtatcggcgccgggacttacatctca3240
aaattaggactgatcaaagcccttaaacacctcggtgagcctctggctacaatgcaatga3300
gcggccgc3308
<210>23
<211>1095
<212>prt
<213>塞内卡病毒
<400>23
metglyasnvalglnthrthrserlysasnasppheaspserarggly
151015
asnasnglyasnmetthrpheasntyrtyralaasnthrtyrglnasn
202530
servalasppheserthrserserseralaserglyalaglyprogly
354045
asnserargglyglyleualaglyleuleuthrasnpheserglyile
505560
leuasnproleuglytyrleulysasphisasnthrgluglumetglu
65707580
asnseralaaspargvalilethrglnthralaglyasnthralaile
859095
asnthrglnserserleuglyvalleucysalatyrvalgluasppro
100105110
thrlysseraspproproserserserthraspglnprothrthrthr
115120125
phethralaileaspargtrptyrthrglyargleuasnsertrpthr
130135140
lysalavallysthrpheserpheglnalavalproleuproglyala
145150155160
pheleuserlysglnglyglyleuasnglyglyalaphethralathr
165170175
leuhisarghispheleumetlyscysglytrpglnvalglnvalgln
180185190
cysasnleuthrglnphehisglnglyalaleuleuvalalametval
195200205
progluthrthrleuaspvallysproaspglylysalalysserleu
210215220
glngluleuasnglugluglntrpvalglumetseraspasptyrarg
225230235240
thrglylysasnmetpropheglnserleuglythrtyrtyrargpro
245250255
proasntrpthrtrpglyproasnpheileasnprotyrglnvalthr
260265270
valpheprohisglnileleuasnalaargthrserthrservalasp
275280285
ileservalprotyrileglygluthrprothrglnsersergluthr
290295300
glnasnsertrpthrleuleuvalmetvalleuvalproleuasptyr
305310315320
lysgluglyalathrthraspprogluilethrpheservalargpro
325330335
thrserprotyrpheasnglyleuargasnargphethrthrglythr
340345350
aspglugluglnglyproileprothralaproarggluasnserleu
355360365
metpheleuserthrileproaspaspthrvalproalatyrglyasn
370375380
valargthrproprovalasntyrleuproglygluilethraspleu
385390395400
leuglnleualaargileprothrleumetalapheglyargvalser
405410415
gluprogluproalaseraspalatyrvalprotyrvalalavalpro
420425430
alaglnpheaspasplysproleuileserpheproilethrleuser
435440445
aspprovaltyrglnasnthrleuvalglyalaileserserasnphe
450455460
alaasntyrargglycysileglnilethrleuthrphecysglypro
465470475480
metmetalaargglylyspheleuleusertyrserproproasngly
485490495
alaglnproglnthrleuserglualametglncysthrtyrserile
500505510
trpaspileglyleuasnsersertrpthrphevalileprotyrile
515520525
serproserasptyrarggluthrargalailethrasnservaltyr
530535540
seralaaspglytrppheserleuhislysleuthrlysilethrleu
545550555560
proproaspcysproglnserprocysileleuphephealaserala
565570575
glygluasptyrthrleuargleuprovalaspcysasnprosertyr
580585590
valproglnglyvalaspasnalagluthrglyvalileglualagly
595600605
asnthraspthrasppheserglygluleualaalaproglyserasn
610615620
histhrasnvallyspheleupheaspargserargleuleuasnval
625630635640
ilelysvalleuglulysaspalavalpheproargpropheprothr
645650655
alathrglyalaglnglnaspaspglytyrphecysleuleuthrpro
660665670
argprothrvalalaserargproalathrargpheglyleutyrval
675680685
asnproseraspserglyvalleualaasnthrserleuasppheasn
690695700
phetyrserleualacysphethrtyrpheargseraspleugluval
705710715720
thrvalvalserleugluproaspleugluphealavalglytrpphe
725730735
proserglyserglutyrglnalaserserphevaltyraspglnleu
740745750
hisvalprotyrhisphethrglyargthrproargalaphethrser
755760765
lysglyglylysvalserphevalleuprotrpasnservalserser
770775780
valleuprovalargtrpglyglyalaserlysleuserseralathr
785790795800
argglyleuproalahisalaasptrpglythriletyralapheile
805810815
proargproasnglulyslysserthralavallyshisvalalaval
820825830
tyrvalargtyrlysasnalaargalatrpcysprosermetleupro
835840845
pheargsertyrlysglnlysmetleumetglnserglyaspileglu
850855860
thrasnproglyproglyserglylysproproglyalaleuserleu
865870875880
metglumetglnglnproasnvalaspmetglypheglualaalaval
885890895
alalyslysvalvalvalproilethrphemetvalproasnargpro
900905910
serglyleuthrglnseralaleuleuvalalaglyargthrpheleu
915920925
ileasngluhisthrtrpserasnprosertrpthrserphethrile
930935940
argglygluvalhisthrargaspglupropheglnthrvalhisphe
945950955960
thrhishisglyleuprothraspleumetmetvalargleuglypro
965970975
glyasnserpheproasnasnleuasplyspheglyleuaspglnmet
980985990
proalaargasnserargvalvalglyvalseralasertyrglyasn
99510001005
phephepheserglyasnpheleuglyphevalaspserilethr
101010151020
seraspglnglythrtyralaargleupheargtyrargvalthr
102510301035
thrtyrlysglytrpcysglyseralaleuvalcysglualagly
104010451050
glyvalargargileileglymethisseralaglyalaalagly
105510601065
ileglyalaglythrtyrileserlysleuglyleuilelysala
107010751080
leulyshisleuglygluproleualathrmetgln
108510901095
<210>24
<211>4694
<212>dna
<213>塞内卡病毒
<400>24
ggatccgccaccatgggtaatgttcagacaacctcaaagaatgattttgattcccgcggc60
aataatggtaacatgaccttcaattactacgcaaacacttaccagaattcagtagacttc120
tcgacctcctcgtcggcgtcaggcgccggacccgggaactcccggggcggactagcgggt180
ctcctcacaaatttcagtggaatcttgaaccctcttggctacctcaaagatcacaatacc240
gaagaaatggaaaactctgctgatcgagtcataacgcaaacggcgggcaacactgccata300
aacacgcaatcatcactgggtgtgttgtgtgcctacgttgaagacccgaccaaatctgac360
cctccgtccagcagcacagatcaacccaccaccacttttactgccatcgacaggtggtac420
actggacgcctcaattcttggacaaaagctgtaaaaaccttctcttttcaggccgtcccg480
ctccctggagccttcctgtctaaacagggaggcctcaacggaggggccttcacggctacc540
ctacatagacatttcttaatgaagtgcgggtggcaggtgcaggtccaatgcaatttgacg600
caattccaccaaggtgctcttcttgttgccatggtccccgaaaccacccttgatgtcaaa660
cctgacggcaaggcaaagagcttacaggagctgaatgaagagcagtgggtggaaatgtct720
gacgattaccggaccgggaaaaacatgccttttcagtctcttggcacatactatcggccc780
cctaactggacttggggccccaatttcatcaacccctatcaagtaacagttttcccacac840
caaattctgaacgcgagaacctctacctcggtagacataagtgtcccatacatcggggag900
actcctacacaatcctcagagacacagaactcctggaccctccttgttatggtgcttgtc960
cccctggactacaaggagggagccacaactgacccagaaattacattttctgtaaggcct1020
acaagtccctacttcaatgggcttcgtaaccgtttcacgaccgggacggacgaggaacag1080
gggcccattcccacagcacccagagaaaattcgcttatgtttctctcaaccatccctgac1140
gacactgtccctgcttacgggaatgtgcgtacccctcccgtcaattacctccccggtgaa1200
ataaccgacctcttacaactggcccgtatacccactctcatggcgtttgggcgggtgtct1260
gaacccgagcctgcctcagacgcatatgtgccctacgttgccgttcctgcccagttcgac1320
gacaagcctctcatctccttcccgatcaccctttcagatcctgtctaccagaacaccctg1380
gtaggcgccatcagttcgaacttcgccaactaccgggggtgtatccaaatcactctgaca1440
ttttgtggacccatgatggcaagagggaaattcctgctctcgtattctcccccaaatgga1500
gcacaaccacagaccctttctgaagctatgcagtgcacatactctatttgggatataggc1560
ttgaactctagttggacctttgtcatcccctacatctcgcccagtgattaccgtgaaact1620
cgggctattaccaactcagtttattctgctgatggttggtttagcttgcacaagctgacc1680
aaaattactctaccacctgactgcccacagagtccctgtattctctttttcgcctctgct1740
ggtgaggattacaccctccgtctccctgttgattgtaatccttcctacgtgttccactcc1800
accgacaacgccgagactggggttattgaggcaggtaacactgacaccgatttctctggt1860
gaactggcggctcctggctctaaccatactaatgtcaaattcctgtttgaccgatctcga1920
ctactgaatgtaattaaggtactggagaaggacgccgtcttcccccgtcctttccccaca1980
gcaacaggtgcacagcaggacgatggttacttttgtcttctaacaccccgcccaacagtc2040
gcttcccgacccgccactcgtttcggcctgtacgtcaacccgtctgacagtggcgttctc2100
gctaacacttcactggatttcaatttttacagtttggcctgtttcacttactttagatca2160
gaccttgaagtcacggtggtctcactggagccagatttggaattcgccgtggggtggttc2220
ccctctggcagtgagtaccaggcttctagctttgtctacgaccaactgcatgtaccctac2280
cactttactgggcgcactccccgcgctttcaccagcaagggtggaaaggtatctttcgtg2340
ctcccttggaactctgtctcttccgtgcttcccgtgcgctgggggggcgcttccaagctt2400
tcttctgccacgcggggtctgccggctcatgctgactgggggaccatttacgcctttatc2460
ccccgtcctaacgagaagaaaagcaccgctgtaaagcacgtggcggtgtacgttcggtac2520
aagaacgcgcgtgcttggtgccccagcatgcttccctttcgcagctacaagcaaaagatg2580
ctgatgcaatcaggcgacatcgagaccaaccctggccctggttcaggtaagccccctgga2640
gcgctctctcttatggaaatgcaacagcccaacgtggacatgggctttgaggctgcggtc2700
gctaagaaagtggtcgtccccattaccttcatggttcccaacagaccttctggacttaca2760
cagtccgctcttcttgtggccggccggaccttcctaatcaatgagcatacatggtccaac2820
ccctcctggaccagcttcacaatccgtggtgaggtgcacactcgtgatgagcctttccaa2880
acggttcattttactcaccatggtcttcccacagatctgatgatggtacgtctcggaccg2940
ggcaactctttccctaacaatctagacaagtttggacttgaccagatgccggcacgtaac3000
tcccgtgtggttggcgtttcggctagttacggtaacttcttcttctctgggaacttcctc3060
gggtttgttgactccatcacctctgaccaaggaacctatgcgagacttttcaggtacagg3120
gtgacgacttacaagggatggtgcggttcggccctggtctgtgaggccggtggtgtccga3180
cgcatcattggcatgcattctgctggtgccgctggtatcggcgccgggacttacatctca3240
aaattaggactgatcaaagcccttaaacacctcggtgagcctctggctacaatgcaagga3300
ctgatgactgagctagagcctggagtcaccgtacatgtaccccgaaaatctaaattgaga3360
aagacgaccgcacacgcggtgtacaaaccggagtttgaacctgctgtgttgtcaaaattt3420
gatcccagactgaacaaggatgttgacctagatgaggtaatttggtctaaacacaccgcc3480
aacgtcccttatcaacctcctttgttctacacatacatgtcagagtacgctcatcgggtt3540
ttctcctttttgggaaaagacaatgacattctgaccgtcaaagaagcaatcctgggcatc3600
cctggactagaccctatggatccccacacagctccgggtttgccctacgccattagcggc3660
cttcgacgtactgatctcgtcgattttgcgaacggcacggtagacccggcactggccatg3720
cagatccagaaattcttagacggtgactactctgatcatgtcttccaaacttttctgaaa3780
gatgaaatcagaccctcagagaaggtccgggcgggaaaaacccgcattgtcgatgtgccc3840
tccctggcgcactgcattgtgggcagaatgctgcttgggcgctttgccgccaagtttcaa3900
tcccatcctggctttctccttggctccgctatcgggtctgaccctgatgtcttctggacc3960
gtcataggggctcagctcgagggaagaaagaacacgtatgacgtggactacagtgccttt4020
gactcttcacacggcactggctccttcgaggctctcatctctcactttttcaccgtggac4080
aatggtttcagccctgcgctgggaccgtatctcagatccctggctgtctcggtgcacgct4140
tacggcgagcgtcgcatcaagattaccggaggcctcccctctggttgtgccgcgaccagc4200
ctgctgaacacagtgctcaacaatgtgatcatcaggactgctctggcattgacctacaag4260
gaatttgaatatgacatggttgatatcatcgcctacggtgacgaccttctggttggtacg4320
gattatgatctggacttcaatgaggtggcgcggcgcgctgccaaactggggtataagatg4380
actcctgccaacaagggttctgtcttccctccgacttcctctctctccgatgctgttttt4440
ctaaaacgcaaattcgtccaaaacaatgacggcttatataaaccagttatggatttaaag4500
aatttggaagccatgctctcctacttcaaaccaggaacactactcgagaagctgcaatct4560
gtttctatgttggctcaacattctggaaaagaagaatacgatagattgatgcaccccttc4620
gctgactatggtgccgtaccgagtcacgagtacctgcaggcaagatggagggccttgttc4680
gactgagcggccgc4694
<210>25
<211>1557
<212>prt
<213>塞内卡病毒
<400>25
metglyasnvalglnthrthrserlysasnasppheaspserarggly
151015
asnasnglyasnmetthrpheasntyrtyralaasnthrtyrglnasn
202530
servalasppheserthrserserseralaserglyalaglyprogly
354045
asnserargglyglyleualaglyleuleuthrasnpheserglyile
505560
leuasnproleuglytyrleulysasphisasnthrgluglumetglu
65707580
asnseralaaspargvalilethrglnthralaglyasnthralaile
859095
asnthrglnserserleuglyvalleucysalatyrvalgluasppro
100105110
thrlysseraspproproserserserthraspglnprothrthrthr
115120125
phethralaileaspargtrptyrthrglyargleuasnsertrpthr
130135140
lysalavallysthrpheserpheglnalavalproleuproglyala
145150155160
pheleuserlysglnglyglyleuasnglyglyalaphethralathr
165170175
leuhisarghispheleumetlyscysglytrpglnvalglnvalgln
180185190
cysasnleuthrglnphehisglnglyalaleuleuvalalametval
195200205
progluthrthrleuaspvallysproaspglylysalalysserleu
210215220
glngluleuasnglugluglntrpvalglumetseraspasptyrarg
225230235240
thrglylysasnmetpropheglnserleuglythrtyrtyrargpro
245250255
proasntrpthrtrpglyproasnpheileasnprotyrglnvalthr
260265270
valpheprohisglnileleuasnalaargthrserthrservalasp
275280285
ileservalprotyrileglygluthrprothrglnsersergluthr
290295300
glnasnsertrpthrleuleuvalmetvalleuvalproleuasptyr
305310315320
lysgluglyalathrthraspprogluilethrpheservalargpro
325330335
thrserprotyrpheasnglyleuargasnargphethrthrglythr
340345350
aspglugluglnglyproileprothralaproarggluasnserleu
355360365
metpheleuserthrileproaspaspthrvalproalatyrglyasn
370375380
valargthrproprovalasntyrleuproglygluilethraspleu
385390395400
leuglnleualaargileprothrleumetalapheglyargvalser
405410415
gluprogluproalaseraspalatyrvalprotyrvalalavalpro
420425430
alaglnpheaspasplysproleuileserpheproilethrleuser
435440445
aspprovaltyrglnasnthrleuvalglyalaileserserasnphe
450455460
alaasntyrargglycysileglnilethrleuthrphecysglypro
465470475480
metmetalaargglylyspheleuleusertyrserproproasngly
485490495
alaglnproglnthrleuserglualametglncysthrtyrserile
500505510
trpaspileglyleuasnsersertrpthrphevalileprotyrile
515520525
serproserasptyrarggluthrargalailethrasnservaltyr
530535540
seralaaspglytrppheserleuhislysleuthrlysilethrleu
545550555560
proproaspcysproglnserprocysileleuphephealaserala
565570575
glygluasptyrthrleuargleuprovalaspcysasnprosertyr
580585590
valphehisserthraspasnalagluthrglyvalileglualagly
595600605
asnthraspthrasppheserglygluleualaalaproglyserasn
610615620
histhrasnvallyspheleupheaspargserargleuleuasnval
625630635640
ilelysvalleuglulysaspalavalpheproargpropheprothr
645650655
alathrglyalaglnglnaspaspglytyrphecysleuleuthrpro
660665670
argprothrvalalaserargproalathrargpheglyleutyrval
675680685
asnproseraspserglyvalleualaasnthrserleuasppheasn
690695700
phetyrserleualacysphethrtyrpheargseraspleugluval
705710715720
thrvalvalserleugluproaspleugluphealavalglytrpphe
725730735
proserglyserglutyrglnalaserserphevaltyraspglnleu
740745750
hisvalprotyrhisphethrglyargthrproargalaphethrser
755760765
lysglyglylysvalserphevalleuprotrpasnservalserser
770775780
valleuprovalargtrpglyglyalaserlysleuserseralathr
785790795800
argglyleuproalahisalaasptrpglythriletyralapheile
805810815
proargproasnglulyslysserthralavallyshisvalalaval
820825830
tyrvalargtyrlysasnalaargalatrpcysprosermetleupro
835840845
pheargsertyrlysglnlysmetleumetglnserglyaspileglu
850855860
thrasnproglyproglyserglylysproproglyalaleuserleu
865870875880
metglumetglnglnproasnvalaspmetglypheglualaalaval
885890895
alalyslysvalvalvalproilethrphemetvalproasnargpro
900905910
serglyleuthrglnseralaleuleuvalalaglyargthrpheleu
915920925
ileasngluhisthrtrpserasnprosertrpthrserphethrile
930935940
argglygluvalhisthrargaspglupropheglnthrvalhisphe
945950955960
thrhishisglyleuprothraspleumetmetvalargleuglypro
965970975
glyasnserpheproasnasnleuasplyspheglyleuaspglnmet
980985990
proalaargasnserargvalvalglyvalseralasertyrglyasn
99510001005
phephepheserglyasnpheleuglyphevalaspserilethr
101010151020
seraspglnglythrtyralaargleupheargtyrargvalthr
102510301035
thrtyrlysglytrpcysglyseralaleuvalcysglualagly
104010451050
glyvalargargileileglymethisseralaglyalaalagly
105510601065
ileglyalaglythrtyrileserlysleuglyleuilelysala
107010751080
leulyshisleuglygluproleualathrmetglnglyleumet
108510901095
thrgluleugluproglyvalthrvalhisvalproarglysser
110011051110
lysleuarglysthrthralahisalavaltyrlysprogluphe
111511201125
gluproalavalleuserlyspheaspproargleuasnlysasp
113011351140
valaspleuaspgluvaliletrpserlyshisthralaasnval
114511501155
protyrglnproproleuphetyrthrtyrmetserglutyrala
116011651170
hisargvalpheserpheleuglylysaspasnaspileleuthr
117511801185
vallysglualaileleuglyileproglyleuaspprometasp
119011951200
prohisthralaproglyleuprotyralaileserglyleuarg
120512101215
argthraspleuvalaspphealaasnglythrvalaspproala
122012251230
leualametglnileglnlyspheleuaspglyasptyrserasp
123512401245
hisvalpheglnthrpheleulysaspgluileargproserglu
125012551260
lysvalargalaglylysthrargilevalaspvalproserleu
126512701275
alahiscysilevalglyargmetleuleuglyargphealaala
128012851290
lyspheglnserhisproglypheleuleuglyseralailegly
129513001305
seraspproaspvalphetrpthrvalileglyalaglnleuglu
131013151320
glyarglysasnthrtyraspvalasptyrseralapheaspser
132513301335
serhisglythrglyserpheglualaleuileserhisphephe
134013451350
thrvalaspasnglypheserproalaleuglyprotyrleuarg
135513601365
serleualavalservalhisalatyrglygluargargilelys
137013751380
ilethrglyglyleuproserglycysalaalathrserleuleu
138513901395
asnthrvalleuasnasnvalileileargthralaleualaleu
140014051410
thrtyrlysglupheglutyraspmetvalaspileilealatyr
141514201425
glyaspaspleuleuvalglythrasptyraspleuasppheasn
143014351440
gluvalalaargargalaalalysleuglytyrlysmetthrpro
144514501455
alaasnlysglyservalpheproprothrserserleuserasp
146014651470
alavalpheleulysarglysphevalglnasnasnaspglyleu
147514801485
tyrlysprovalmetaspleulysasnleuglualametleuser
149014951500
tyrphelysproglythrleuleuglulysleuglnservalser
150515101515
metleualaglnhisserglylysgluglutyraspargleumet
152015251530
hisprophealaasptyrglyalavalproserhisglutyrleu
153515401545
glnalaargtrpargalaleupheasp
15501555
<210>26
<211>2618
<212>dna
<213>塞内卡病毒
<400>26
ggatccgccaccatgggtaatgttcagacaacctcaaagaatgattttgattcccgcggc60
aataatggtaacatgaccttcaattactacgcaaacacttaccagaattcagtagacttc120
tcgacctcctcgtcggcgtcaggcgccggacccgggaactcccggggcggactagcgggt180
ctcctcacaaatttcagtggaatcttgaaccctcttggctacctcaaagatcacaatacc240
gaagaaatggaaaactctgctgatcgagtcataacgcaaacggcgggcaacactgccata300
aacacgcaatcatcactgggtgtgttgtgtgcctacgttgaagacccgaccaaatctgac360
cctccgtccagcagcacagatcaacccaccaccacttttactgccatcgacaggtggtac420
actggacgcctcaattcttggacaaaagctgtaaaaaccttctcttttcaggccgtcccg480
ctccctggagccttcctgtctaaacagggaggcctcaacggaggggccttcacggctacc540
ctacatagacatttcttaatgaagtgcgggtggcaggtgcaggtccaatgcaatttgacg600
caattccaccaaggtgctcttcttgttgccatggtccccgaaaccacccttgatgtcaaa660
cctgacggcaaggcaaagagcttacaggagctgaatgaagagcagtgggtggaaatgtct720
gacgattaccggaccgggaaaaacatgccttttcagtctcttggcacatactatcggccc780
cctaactggacttggggccccaatttcatcaacccctatcaagtaacagttttcccacac840
caaattctgaacgcgagaacctctacctcggtagacataagtgtcccatacatcggggag900
actcctacacaatcctcagagacacagaactcctggaccctccttgttatggtgcttgtc960
cccctggactacaaggagggagccacaactgacccagaaattacattttctgtaaggcct1020
acaagtccctacttcaatgggcttcgtaaccgtttcacgaccgggacggacgaggaacag1080
gggcccattcccacagcacccagagaaaattcgcttatgtttctctcaaccatccctgac1140
gacactgtccctgcttacgggaatgtgcgtacccctcccgtcaattacctccccggtgaa1200
ataaccgacctcttacaactggcccgtatacccactctcatggcgtttgggcgggtgtct1260
gaacccgagcctgcctcagacgcatatgtgccctacgttgccgttcctgcccagttcgac1320
gacaagcctctcatctccttcccgatcaccctttcagatcctgtctaccagaacaccctg1380
gtaggcgccatcagttcgaacttcgccaactaccgggggtgtatccaaatcactctgaca1440
ttttgtggacccatgatggcaagagggaaattcctgctctcgtattctcccccaaatgga1500
gcacaaccacagaccctttctgaagctatgcagtgcacatactctatttgggatataggc1560
ttgaactctagttggacctttgtcatcccctacatctcgcccagtgattaccgtgaaact1620
cgggctattaccaactcagtttattctgctgatggttggtttagcttgcacaagctgacc1680
aaaattactctaccacctgactgcccacagagtccctgtattctctttttcgcctctgct1740
ggtgaggattacaccctccgtctccctgttgattgtaatccttcctacgtgttccactcc1800
accgacaacgccgagactggggttattgaggcaggtaacactgacaccgatttctctggt1860
gaactggcggctcctggctctaaccatactaatgtcaaattcctgtttgaccgatctcga1920
ctactgaatgtaattaaggtactggagaaggacgccgtcttcccccgtcctttccccaca1980
gcaacaggtgcacagcaggacgatggttacttttgtcttctaacaccccgcccaacagtc2040
gcttcccgacccgccactcgtttcggcctgtacgtcaacccgtctgacagtggcgttctc2100
gctaacacttcactggatttcaatttttacagtttggcctgtttcacttactttagatca2160
gaccttgaagtcacggtggtctcactggagccagatttggaattcgccgtggggtggttc2220
ccctctggcagtgagtaccaggcttctagctttgtctacgaccaactgcatgtaccctac2280
cactttactgggcgcactccccgcgctttcaccagcaagggtggaaaggtatctttcgtg2340
ctcccttggaactctgtctcttccgtgcttcccgtgcgctgggggggcgcttccaagctt2400
tcttctgccacgcggtgtctgccggctcatgctgactgggggaccatttacgcctttatc2460
ccccgtcctaacgagaagaaaagcaccgctgtaaagcacgtggcggtgtacgttcggtac2520
aagaacgcgcgtgcttggtgccccagcatgcttccctttcgcagctacaagcaaaagatg2580
ctgatgcaacaccaccaccaccaccactgagcggccgc2618
<210>27
<211>855
<212>prt
<213>塞内卡病毒
<400>27
metglyasnvalglnthrthrserlysasnasppheaspserarggly
151015
asnasnglyasnmetthrpheasntyrtyralaasnthrtyrglnasn
202530
servalasppheserthrserserseralaserglyalaglyprogly
354045
asnserargglyglyleualaglyleuleuthrasnpheserglyile
505560
leuasnproleuglytyrleulysasphisasnthrgluglumetglu
65707580
asnseralaaspargvalilethrglnthralaglyasnthralaile
859095
asnthrglnserserleuglyvalleucysalatyrvalgluasppro
100105110
thrlysseraspproproserserserthraspglnprothrthrthr
115120125
phethralaileaspargtrptyrthrglyargleuasnsertrpthr
130135140
lysalavallysthrpheserpheglnalavalproleuproglyala
145150155160
pheleuserlysglnglyglyleuasnglyglyalaphethralathr
165170175
leuhisarghispheleumetlyscysglytrpglnvalglnvalgln
180185190
cysasnleuthrglnphehisglnglyalaleuleuvalalametval
195200205
progluthrthrleuaspvallysproaspglylysalalysserleu
210215220
glngluleuasnglugluglntrpvalglumetseraspasptyrarg
225230235240
thrglylysasnmetpropheglnserleuglythrtyrtyrargpro
245250255
proasntrpthrtrpglyproasnpheileasnprotyrglnvalthr
260265270
valpheprohisglnileleuasnalaargthrserthrservalasp
275280285
ileservalprotyrileglygluthrprothrglnsersergluthr
290295300
glnasnsertrpthrleuleuvalmetvalleuvalproleuasptyr
305310315320
lysgluglyalathrthraspprogluilethrpheservalargpro
325330335
thrserprotyrpheasnglyleuargasnargphethrthrglythr
340345350
aspglugluglnglyproileprothralaproarggluasnserleu
355360365
metpheleuserthrileproaspaspthrvalproalatyrglyasn
370375380
valargthrproprovalasntyrleuproglygluilethraspleu
385390395400
leuglnleualaargileprothrleumetalapheglyargvalser
405410415
gluprogluproalaseraspalatyrvalprotyrvalalavalpro
420425430
alaglnpheaspasplysproleuileserpheproilethrleuser
435440445
aspprovaltyrglnasnthrleuvalglyalaileserserasnphe
450455460
alaasntyrargglycysileglnilethrleuthrphecysglypro
465470475480
metmetalaargglylyspheleuleusertyrserproproasngly
485490495
alaglnproglnthrleuserglualametglncysthrtyrserile
500505510
trpaspileglyleuasnsersertrpthrphevalileprotyrile
515520525
serproserasptyrarggluthrargalailethrasnservaltyr
530535540
seralaaspglytrppheserleuhislysleuthrlysilethrleu
545550555560
proproaspcysproglnserprocysileleuphephealaserala
565570575
glygluasptyrthrleuargleuprovalaspcysasnprosertyr
580585590
valphehisserthraspasnalagluthrglyvalileglualagly
595600605
asnthraspthrasppheserglygluleualaalaproglyserasn
610615620
histhrasnvallyspheleupheaspargserargleuleuasnval
625630635640
ilelysvalleuglulysaspalavalpheproargpropheprothr
645650655
alathrglyalaglnglnaspaspglytyrphecysleuleuthrpro
660665670
argprothrvalalaserargproalathrargpheglyleutyrval
675680685
asnproseraspserglyvalleualaasnthrserleuasppheasn
690695700
phetyrserleualacysphethrtyrpheargseraspleugluval
705710715720
thrvalvalserleugluproaspleugluphealavalglytrpphe
725730735
proserglyserglutyrglnalaserserphevaltyraspglnleu
740745750
hisvalprotyrhisphethrglyargthrproargalaphethrser
755760765
lysglyglylysvalserphevalleuprotrpasnservalserser
770775780
valleuprovalargtrpglyglyalaserlysleuserseralathr
785790795800
argcysleuproalahisalaasptrpglythriletyralapheile
805810815
proargproasnglulyslysserthralavallyshisvalalaval
820825830
tyrvalargtyrlysasnalaargalatrpcysprosermetleupro
835840845
pheargsertyrlysglnlys
850855
<210>28
<211>2618
<212>dna
<213>塞内卡病毒
<400>28
ggatccgccaccatgggcaacgttcaaactacctccaagaatgattttgactcccgcggc60
aacaacggcaacatgacttttaactactacgccaatacgtatcaaaactctgttgatttc120
tcgacatcgagttccgcttccggcgcgggacctggaaattcccgcggtggactggccggt180
ctgctcacaaacttttccggtatcctgaaccccctcggttatctgaaagatcacaatacg240
gaagagatggagaactctgccgatcgtgtgattacgcagacagctggtaacacggctatc300
aacactcaatcgtctctgggagtactctgtgcttacgtcgaggacccaacaaaatccgac360
cctccatcttcctcgaccgatcaacctacgactaccttcacggccatagacaggtggtat420
acaggtcgcctgaattcgtggacaaaggctgttaaaacctttagcttccaggctgtgcca480
ttgcccggtgctttcctttcccgccagggcggcctcaacggtggagcttttactgctaca540
ctgcacagacactttctgatgaaatgcggttggcaggtgcaggtacagtgcaatttgact600
caattccaccaaggtgcacttctcgttgctatggttcccgagactacgcttgacgttaag660
cctgacggcaaggccaagtctctccaagagctgaatgaggaacagtgggttgagatgagc720
gatgactatagaaccggtaaaaacatgccattccagtccctgggcacatactacagaccc780
ccaaattggacttggggcccgaactttatcaatccgtatcaggtcaccgtgtttccacac840
caaatcctgaatgccaggactagtaccagcgtcgatattagcgtgccctatatcggcgaa900
acgccaacccaatcctccgaaactcagaacagctggacgcttctggtaatggtgttggta960
cccttggattataaggagggagccactaccgaccctgaaatcactttttcggtaaggccc1020
actagcccttacttcaacggactgcgcaaccgtttcaccaccggcactgatgaggagcaa1080
ggccctatccctactgcccccagagaaaatagcctgatgtttctgagcacaattcctgac1140
gacaccgtacctgcctatggtaacgttcgtacgcctcccgtaaactacctgcctggtgaa1200
attacggacctcttgcagctggcccgcatccctacactgatggccttcggtcgtgtcagt1260
gagcccgaaccagccagcgacgcttacgttccatatgttgcggtacctgctcagttcgac1320
gacaagccgttgataagtttcccaatcactttgagcgaccctgtataccagaacactttg1380
gttggagctatctcctcgaactttgcgaactaccgtggttgtattcaaatcacccttaca1440
ttctgcggcccgatgatggctcgtggcaagttcctgttgagctactccccccccaacgga1500
gcacaaccacagacactgtccgaggccatgcagtgcacatactccatctgggatatcggt1560
ctcaacagctcatggacctttgtaataccatacatctcgccatccgattatagagagacc1620
cgcgccatcaccaacagtgtgtactcagccgatggatggttcagtctccataagcttaca1680
aagatcactctgccccccgactgcccacaatcaccctgcatattgttctttgcctcagct1740
ggtgaagattacacgcttcgtttgcccgtcgactgtaacccgtcttacgtgttccactcc1800
acggacaatgctgagaccggtgtaattgaggctggtaacaccgacacggatttctctggt1860
gagctggcggctcccggttctaaccataccaacgtgaagttccttttcgaccgttcacgt1920
cttttgaacgtaatcaaggtcttggaaaaggacgctgtgttccctcgcccattccccaca1980
gcaacgggagcacaacaagacgacggctatttctgcctgctgacaccacgtcctactgtg2040
gctagccgtccggccacacgctttggtctctacgttaaccctagcgactctggtgtactg2100
gctaacacctccctggacttcaatttctactctctggcctgcttcacgtacttcaggagc2160
gacctggaggtcactgtggtgtctctggaaccagatctggaatttgcggtaggatggttc2220
ccttcaggaagcgagtaccaggcatcgtcctttgtgtacgaccaacttcatgttccctac2280
catttcaccggtagaaccccacgtgccttcaccagtaagggaggcaaagtttcttttgta2340
ctgccctggaattctgtcagttccgttctcccggtccgctggggcggagcgtcaaagctc2400
tcatccgcgacccgcggattgcccgcacacgcggattggggaactatctacgctttcatt2460
ccacgccctaatgaaaagaagtctacagcagtgaagcacgtggccgtctatgtgcgttac2520
aagaatgcaagggcgtggtgtccgtctatgttgccattccgtagctataaacagaagatg2580
cttatgcaacaccaccaccaccaccactgagcggccgc2618
<210>29
<211>865
<212>prt
<213>塞内卡病毒
<400>29
metglyasnvalglnthrthrserlysasnasppheaspserarggly
151015
asnasnglyasnmetthrpheasntyrtyralaasnthrtyrglnasn
202530
servalasppheserthrserserseralaserglyalaglyprogly
354045
asnserargglyglyleualaglyleuleuthrasnpheserglyile
505560
leuasnproleuglytyrleulysasphisasnthrgluglumetglu
65707580
asnseralaaspargvalilethrglnthralaglyasnthralaile
859095
asnthrglnserserleuglyvalleucysalatyrvalgluasppro
100105110
thrlysseraspproproserserserthraspglnprothrthrthr
115120125
phethralaileaspargtrptyrthrglyargleuasnsertrpthr
130135140
lysalavallysthrpheserpheglnalavalproleuproglyala
145150155160
pheleuserargglnglyglyleuasnglyglyalaphethralathr
165170175
leuhisarghispheleumetlyscysglytrpglnvalglnvalgln
180185190
cysasnleuthrglnphehisglnglyalaleuleuvalalametval
195200205
progluthrthrleuaspvallysproaspglylysalalysserleu
210215220
glngluleuasnglugluglntrpvalglumetseraspasptyrarg
225230235240
thrglylysasnmetpropheglnserleuglythrtyrtyrargpro
245250255
proasntrpthrtrpglyproasnpheileasnprotyrglnvalthr
260265270
valpheprohisglnileleuasnalaargthrserthrservalasp
275280285
ileservalprotyrileglygluthrprothrglnsersergluthr
290295300
glnasnsertrpthrleuleuvalmetvalleuvalproleuasptyr
305310315320
lysgluglyalathrthraspprogluilethrpheservalargpro
325330335
thrserprotyrpheasnglyleuargasnargphethrthrglythr
340345350
aspglugluglnglyproileprothralaproarggluasnserleu
355360365
metpheleuserthrileproaspaspthrvalproalatyrglyasn
370375380
valargthrproprovalasntyrleuproglygluilethraspleu
385390395400
leuglnleualaargileprothrleumetalapheglyargvalser
405410415
gluprogluproalaseraspalatyrvalprotyrvalalavalpro
420425430
alaglnpheaspasplysproleuileserpheproilethrleuser
435440445
aspprovaltyrglnasnthrleuvalglyalaileserserasnphe
450455460
alaasntyrargglycysileglnilethrleuthrphecysglypro
465470475480
metmetalaargglylyspheleuleusertyrserproproasngly
485490495
alaglnproglnthrleuserglualametglncysthrtyrserile
500505510
trpaspileglyleuasnsersertrpthrphevalileprotyrile
515520525
serproserasptyrarggluthrargalailethrasnservaltyr
530535540
seralaaspglytrppheserleuhislysleuthrlysilethrleu
545550555560
proproaspcysproglnserprocysileleuphephealaserala
565570575
glygluasptyrthrleuargleuprovalaspcysasnprosertyr
580585590
valphehisserthraspasnalagluthrglyvalileglualagly
595600605
asnthraspthrasppheserglygluleualaalaproglyserasn
610615620
histhrasnvallyspheleupheaspargserargleuleuasnval
625630635640
ilelysvalleuglulysaspalavalpheproargpropheprothr
645650655
alathrglyalaglnglnaspaspglytyrphecysleuleuthrpro
660665670
argprothrvalalaserargproalathrargpheglyleutyrval
675680685
asnproseraspserglyvalleualaasnthrserleuasppheasn
690695700
phetyrserleualacysphethrtyrpheargseraspleugluval
705710715720
thrvalvalserleugluproaspleugluphealavalglytrpphe
725730735
proserglyserglutyrglnalaserserphevaltyraspglnleu
740745750
hisvalprotyrhisphethrglyargthrproargalaphethrser
755760765
lysglyglylysvalserphevalleuprotrpasnservalserser
770775780
valleuprovalargtrpglyglyalaserlysleuserseralathr
785790795800
argglyleuproalahisalaasptrpglythriletyralapheile
805810815
proargproasnglulyslysserthralavallyshisvalalaval
820825830
tyrvalargtyrlysasnalaargalatrpcysprosermetleupro
835840845
pheargsertyrlysglnlysmetleumetglnhishishishishis
850855860
his
865
<210>30
<211>651
<212>dna
<213>塞内卡病毒
<400>30
actagtatgcagcccaacgtggacatgggctttgaggctgcggtcgctaagaaagtggtc60
gtccccattaccttcatggttcccaacagaccttctggacttacacagtccgctcttctt120
gtggccggccggaccttcataatcaatgagcatacatggtccaacccctcctggaccagc180
ttcacaatccgtggtgaggtgcacactcgtgatgagcctttccaaacggttcattttact240
caccatggtcttcccacagatctgatgatggtacgtctcggaccgggcaactctttccct300
aacaatctagacaagtttggacttgaccagatgccggcacgtaactcccgtgtggttggc360
gtttcggctagttacggtaacttcttcttctctgggaacttcctcgggtttgttgactcc420
atcacctctgaccaaggaacctatgcgagacttttcaggtacagggtgacgacttacaag480
ggatggtgcggttcggccctggtctgtgaggccggtggtgtccgacgcatcattggcatg540
cattctgctggtgccgctggtatcggcgccgggacttacatctcaaaattaggactgatc600
aaagcccttaaacacctcggtgagcctctggctacaatgcaatgagagctc651
<210>31
<211>212
<212>prt
<213>塞内卡病毒
<400>31
metglnproasnvalaspmetglypheglualaalavalalalyslys
151015
valvalvalproilethrphemetvalproasnargproserglyleu
202530
thrglnseralaleuleuvalalaglyargthrpheileileasnglu
354045
histhrtrpserasnprosertrpthrserphethrileargglyglu
505560
valhisthrargaspglupropheglnthrvalhisphethrhishis
65707580
glyleuprothraspleumetmetvalargleuglyproglyasnser
859095
pheproasnasnleuasplyspheglyleuaspglnmetproalaarg
100105110
asnserargvalvalglyvalseralasertyrglyasnphephephe
115120125
serglyasnpheleuglyphevalaspserilethrseraspglngly
130135140
thrtyralaargleupheargtyrargvalthrthrtyrlysglytrp
145150155160
cysglyseralaleuvalcysglualaglyglyvalargargileile
165170175
glymethisseralaglyalaalaglyileglyalaglythrtyrile
180185190
serlysleuglyleuilelysalaleulyshisleuglygluproleu
195200205
alathrmetgln
210
<210>32
<211>3489
<212>dna
<213>塞内卡病毒
<400>32
ggatccgccaccatgggtaatgttcagacaacctcaaagaatgattttgattcccgcggc60
aataatggtaacatgaccttcaattactacgcaaacacttaccagaattcagtagacttc120
tcgacctcctcgtcggcgtcaggcgccggacccgggaactcccggggcggactagcgggt180
ctcctcacaaatttcagtggaatcttgaaccctcttggctacctcaaagatcacaatacc240
gaagaaatggaaaactctgctgatcgagtcataacgcaaacggcgggcaacactgccata300
aacacgcaatcatcactgggtgtgttgtgtgcctacgttgaagacccgaccaaatctgac360
cctccgtccagcagcacagatcaacccaccaccacttttactgccatcgacaggtggtac420
actggacgcctcaattcttggacaaaagctgtaaaaaccttctcttttcaggccgtcccg480
ctccctggagccttcctgtctaaacagggaggcctcaacggaggggccttcacggctacc540
ctacatagacatttcttaatgaagtgcgggtggcaggtgcaggtccaatgcaatttgacg600
caattccaccaaggtgctcttcttgttgccatggtccccgaaaccacccttgatgtcaaa660
cctgacggcaaggcaaagagcttacaggagctgaatgaagagcagtgggtggaaatgtct720
gacgattaccggaccgggaaaaacatgccttttcagtctcttggcacatactatcggccc780
cctaactggacttggggccccaatttcatcaacccctatcaagtaacagttttcccacac840
caaattctgaacgcgagaacctctacctcggtagacataagtgtcccatacatcggggag900
actcctacacaatcctcagagacacagaactcctggaccctccttgttatggtgcttgtc960
cccctggactacaaggagggagccacaactgacccagaaattacattttctgtaaggcct1020
acaagtccctacttcaatgggcttcgtaaccgtttcacgaccgggacggacgaggaacag1080
gggcccattcccacagcacccagagaaaattcgcttatgtttctctcaaccatccctgac1140
gacactgtccctgcttacgggaatgtgcgtacccctcccgtcaattacctccccggtgaa1200
ataaccgacctcttacaactggcccgtatacccactctcatggcgtttgggcgggtgtct1260
gaacccgagcctgcctcagacgcatatgtgccctacgttgccgttcctgcccagttcgac1320
gacaagcctctcatctccttcccgatcaccctttcagatcctgtctaccagaacaccctg1380
gtaggcgccatcagttcgaacttcgccaactaccgggggtgtatccaaatcactctgaca1440
ttttgtggacccatgatggcaagagggaaattcctgctctcgtattctcccccaaatgga1500
gcacaaccacagaccctttctgaagctatgcagtgcacatactctatttgggatataggc1560
ttgaactctagttggacctttgtcatcccctacatctcgcccagtgattaccgtgaaact1620
cgggctattaccaactcagtttattctgctgatggttggtttagcttgcacaagctgacc1680
aaaattactctaccacctgactgcccacagagtccctgtattctctttttcgcctctgct1740
ggtgaggattacaccctccgtctccctgttgattgtaatccttcctacgtgttccactcc1800
accgacaacgccgagactggggttattgaggcaggtaacactgacaccgatttctctggt1860
gaactggcggctcctggctctaaccatactaatgtcaaattcctgtttgaccgatctcga1920
ctactgaatgtaattaaggtactggagaaggacgccgtcttcccccgtcctttccccaca1980
gcaacaggtgcacagcaggacgatggttacttttgtcttctaacaccccgcccaacagtc2040
gcttcccgacccgccactcgtttcggcctgtacgtcaacccgtctgacagtggcgttctc2100
gctaacacttcactggatttcaatttttacagtttggcctgtttcacttactttagatca2160
gaccttgaagtcacggtggtctcactggagccagatttggaattcgccgtggggtggttc2220
ccctctggcagtgagtaccaggcttctagctttgtctacgaccaactgcatgtaccctac2280
cactttactgggcgcactccccgcgctttcaccagcaagggtggaaaggtatctttcgtg2340
ctcccttggaactctgtctcttccgtgcttcccgtgcgctgggggggcgcttccaagctt2400
tcttctgccacgcggtgtctgccggctcatgctgactgggggaccatttacgcctttatc2460
ccccgtcctaacgagaagaaaagcaccgctgtaaagcacgtggcggtgtacgttcggtac2520
aagaacgcgcgtgcttggtgccccagcatgcttccctttcgcagctacaagcaaaagatg2580
ctgatgcaacaccaccaccaccaccactgagcggccgcgaactgtggagccagggcggcc2640
ttggccaccctggcttactgtaacagaaaaaagagtaaaaggcgacagctcgcttgccaa2700
ttgtcctgttacgtactctgtggtttcacgaggttgtcatcaccaaaggtaacctttttt2760
tttgtcctcgccgacaaaacgacatcttaataaccaagcaacgttcgataaagaaaaaaa2820
ctcgtccttaagtcgcgaactagtatgcagcccaacgtggacatgggctttgaggctgcg2880
gtcgctaagaaagtggtcgtccccattaccttcatggttcccaacagaccttctggactt2940
acacagtccgctcttcttgtggccggccggaccttcataatcaatgagcatacatggtcc3000
aacccctcctggaccagcttcacaatccgtggtgaggtgcacactcgtgatgagcctttc3060
caaacggttcattttactcaccatggtcttcccacagatctgatgatggtacgtctcgga3120
ccgggcaactctttccctaacaatctagacaagtttggacttgaccagatgccggcacgt3180
aactcccgtgtggttggcgtttcggctagttacggtaacttcttcttctctgggaacttc3240
ctcgggtttgttgactccatcacctctgaccaaggaacctatgcgagacttttcaggtac3300
agggtgacgacttacaagggatggtgcggttcggccctggtctgtgaggccggtggtgtc3360
cgacgcatcattggcatgcattctgctggtgccgctggtatcggcgccgggacttacatc3420
tcaaaattaggactgatcaaagcccttaaacacctcggtgagcctctggctacaatgcaa3480
tgagagctc3489
<210>33
<211>3489
<212>dna
<213>塞内卡病毒
<400>33
ggatccgccaccatgggcaacgttcaaactacctccaagaatgattttgactcccgcggc60
aacaacggcaacatgacttttaactactacgccaatacgtatcaaaactctgttgatttc120
tcgacatcgagttccgcttccggcgcgggacctggaaattcccgcggtggactggccggt180
ctgctcacaaacttttccggtatcctgaaccccctcggttatctgaaagatcacaatacg240
gaagagatggagaactctgccgatcgtgtgattacgcagacagctggtaacacggctatc300
aacactcaatcgtctctgggagtactctgtgcttacgtcgaggacccaacaaaatccgac360
cctccatcttcctcgaccgatcaacctacgactaccttcacggccatagacaggtggtat420
acaggtcgcctgaattcgtggacaaaggctgttaaaacctttagcttccaggctgtgcca480
ttgcccggtgctttcctttcccgccagggcggcctcaacggtggagcttttactgctaca540
ctgcacagacactttctgatgaaatgcggttggcaggtgcaggtacagtgcaatttgact600
caattccaccaaggtgcacttctcgttgctatggttcccgagactacgcttgacgttaag660
cctgacggcaaggccaagtctctccaagagctgaatgaggaacagtgggttgagatgagc720
gatgactatagaaccggtaaaaacatgccattccagtccctgggcacatactacagaccc780
ccaaattggacttggggcccgaactttatcaatccgtatcaggtcaccgtgtttccacac840
caaatcctgaatgccaggactagtaccagcgtcgatattagcgtgccctatatcggcgaa900
acgccaacccaatcctccgaaactcagaacagctggacgcttctggtaatggtgttggta960
cccttggattataaggagggagccactaccgaccctgaaatcactttttcggtaaggccc1020
actagcccttacttcaacggactgcgcaaccgtttcaccaccggcactgatgaggagcaa1080
ggccctatccctactgcccccagagaaaatagcctgatgtttctgagcacaattcctgac1140
gacaccgtacctgcctatggtaacgttcgtacgcctcccgtaaactacctgcctggtgaa1200
attacggacctcttgcagctggcccgcatccctacactgatggccttcggtcgtgtcagt1260
gagcccgaaccagccagcgacgcttacgttccatatgttgcggtacctgctcagttcgac1320
gacaagccgttgataagtttcccaatcactttgagcgaccctgtataccagaacactttg1380
gttggagctatctcctcgaactttgcgaactaccgtggttgtattcaaatcacccttaca1440
ttctgcggcccgatgatggctcgtggcaagttcctgttgagctactccccccccaacgga1500
gcacaaccacagacactgtccgaggccatgcagtgcacatactccatctgggatatcggt1560
ctcaacagctcatggacctttgtaataccatacatctcgccatccgattatagagagacc1620
cgcgccatcaccaacagtgtgtactcagccgatggatggttcagtctccataagcttaca1680
aagatcactctgccccccgactgcccacaatcaccctgcatattgttctttgcctcagct1740
ggtgaagattacacgcttcgtttgcccgtcgactgtaacccgtcttacgtgttccactcc1800
acggacaatgctgagaccggtgtaattgaggctggtaacaccgacacggatttctctggt1860
gagctggcggctcccggttctaaccataccaacgtgaagttccttttcgaccgttcacgt1920
cttttgaacgtaatcaaggtcttggaaaaggacgctgtgttccctcgcccattccccaca1980
gcaacgggagcacaacaagacgacggctatttctgcctgctgacaccacgtcctactgtg2040
gctagccgtccggccacacgctttggtctctacgttaaccctagcgactctggtgtactg2100
gctaacacctccctggacttcaatttctactctctggcctgcttcacgtacttcaggagc2160
gacctggaggtcactgtggtgtctctggaaccagatctggaatttgcggtaggatggttc2220
ccttcaggaagcgagtaccaggcatcgtcctttgtgtacgaccaacttcatgttccctac2280
catttcaccggtagaaccccacgtgccttcaccagtaagggaggcaaagtttcttttgta2340
ctgccctggaattctgtcagttccgttctcccggtccgctggggcggagcgtcaaagctc2400
tcatccgcgacccgcggattgcccgcacacgcggattggggaactatctacgctttcatt2460
ccacgccctaatgaaaagaagtctacagcagtgaagcacgtggccgtctatgtgcgttac2520
aagaatgcaagggcgtggtgtccgtctatgttgccattccgtagctataaacagaagatg2580
cttatgcaacaccaccaccaccaccactgagcggccgcgaactgtggagccagggcggcc2640
ttggccaccctggcttactgtaacagaaaaaagagtaaaaggcgacagctcgcttgccaa2700
ttgtcctgttacgtactctgtggtttcacgaggttgtcatcaccaaaggtaacctttttt2760
tttgtcctcgccgacaaaacgacatcttaataaccaagcaacgttcgataaagaaaaaaa2820
ctcgtccttaagtcgcgaactagtatgcagcccaacgtggacatgggctttgaggctgcg2880
gtcgctaagaaagtggtcgtccccattaccttcatggttcccaacagaccttctggactt2940
acacagtccgctcttcttgtggccggccggaccttcataatcaatgagcatacatggtcc3000
aacccctcctggaccagcttcacaatccgtggtgaggtgcacactcgtgatgagcctttc3060
caaacggttcattttactcaccatggtcttcccacagatctgatgatggtacgtctcgga3120
ccgggcaactctttccctaacaatctagacaagtttggacttgaccagatgccggcacgt3180
aactcccgtgtggttggcgtttcggctagttacggtaacttcttcttctctgggaacttc3240
ctcgggtttgttgactccatcacctctgaccaaggaacctatgcgagacttttcaggtac3300
agggtgacgacttacaagggatggtgcggttcggccctggtctgtgaggccggtggtgtc3360
cgacgcatcattggcatgcattctgctggtgccgctggtatcggcgccgggacttacatc3420
tcaaaattaggactgatcaaagcccttaaacacctcggtgagcctctggctacaatgcaa3480
tgagagctc3489
<210>34
<211>26
<212>dna
<213>人工序列
<220>
<223>引物
<400>34
ggatccgccaccatgggtaatgttca26
<210>35
<211>58
<212>dna
<213>人工序列
<220>
<223>引物
<400>35
gcggccgctcagtggtggtggtggtggtgttgcatcagcatcttttgcttgtagctgc58
<210>36
<211>22
<212>dna
<213>人工序列
<220>
<223>引物
<400>36
ggatccgccaccatgggcaacg22
<210>37
<211>60
<212>dna
<213>人工序列
<220>
<223>引物
<400>37
gcggccgctcagtggtggtggtggtggtgttgcataagcatcttctgtttatagctacgg60
<210>38
<211>33
<212>dna
<213>人工序列
<220>
<223>引物
<400>38
actagtatgcagcccaacgtggacatgggcttt33
<210>39
<211>35
<212>dna
<213>人工序列
<220>
<223>引物
<400>39
gagctctcattgcattgtagccagaggctcaccga35
<210>40
<211>41
<212>dna
<213>人工序列
<220>
<223>引物
<400>40
cttcctacgtgcctcagggggttgacaacgccgagactggg41
<210>41
<211>41
<212>dna
<213>人工序列
<220>
<223>引物
<400>41
cccagtctcggcgttgtcaaccccctgaggcacgtaggaag41
<210>42
<211>35
<212>dna
<213>人工序列
<220>
<223>引物
<400>42
tacaatgcaaggactgatgactgagctagagcctg35
<210>43
<211>31
<212>dna
<213>人工序列
<220>
<223>引物
<400>43
tcagtcatcagtccttgcattgtagccagag31
<210>44
<211>32
<212>dna
<213>人工序列
<220>
<223>引物
<400>44
gcggccgctcagtcgaacaaggccctccatct32
<210>45
<211>1389
<212>dna
<213>塞内卡病毒
<400>45
ggactgatgactgagctagagcctggagtcaccgtacatgtaccccgaaaatctaaattg60
agaaagacgaccgcacacgcggtgtacaaaccggagtttgaacctgctgtgttgtcaaaa120
tttgatcccagactgaacaaggatgttgacctagatgaggtaatttggtctaaacacacc180
gccaacgtcccttatcaacctcctttgttctacacatacatgtcagagtacgctcatcgg240
gttttctcctttttgggaaaagacaatgacattctgaccgtcaaagaagcaatcctgggc300
atccctggactagaccctatggatccccacacagctccgggtttgccctacgccattagc360
ggccttcgacgtactgatctcgtcgattttgcgaacggcacggtagacccggcactggcc420
atgcagatccagaaattcttagacggtgactactctgatcatgtcttccaaacttttctg480
aaagatgaaatcagaccctcagagaaggtccgggcgggaaaaacccgcattgtcgatgtg540
ccctccctggcgcactgcattgtgggcagaatgctgcttgggcgctttgccgccaagttt600
caatcccatcctggctttctccttggctccgctatcgggtctgaccctgatgtcttctgg660
accgtcataggggctcagctcgagggaagaaagaacacgtatgacgtggactacagtgcc720
tttgactcttcacacggcactggctccttcgaggctctcatctctcactttttcaccgtg780
gacaatggtttcagccctgcgctgggaccgtatctcagatccctggctgtctcggtgcac840
gcttacggcgagcgtcgcatcaagattaccggaggcctcccctctggttgtgccgcgacc900
agcctgctgaacacagtgctcaacaatgtgatcatcaggactgctctggcattgacctac960
aaggaatttgaatatgacatggttgatatcatcgcctacggtgacgaccttctggttggt1020
acggattatgatctggacttcaatgaggtggcgcggcgcgctgccaaactggggtataag1080
atgactcctgccaacaagggttctgtcttccctccgacttcctctctctccgatgctgtt1140
tttctaaaacgcaaattcgtccaaaacaatgacggcttatataaaccagttatggattta1200
aagaatttggaagccatgctctcctacttcaaaccaggaacactactcgagaagctgcaa1260
tctgtttctatgttggctcaacattctggaaaagaagaatacgatagattgatgcacccc1320
ttcgctgactatggtgccgtaccgagtcacgagtacctgcaggcaagatggagggccttg1380
ttcgactga1389
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