在植物中产生二倍体配子的TDM基因中的显性突变的制作方法

本发明涉及：引起在植物中产生2n配子的TDM基因中的显性突变；包含所述突变的植物；以及它们在植物育种中的应用。本发明还涉及这样的植物，在该植物中，TDM基因中的显性突变与以下基因的失活组合：参与植物中减数分裂重组的基因，和参与减数分裂期间着丝粒的单极取向的基因。这些产生未减数分裂配子的植物也可用于植物育种。

背景技术：

减数分裂是有性繁殖的真核生物(诸如，大多数的开花植物)生命周期中的关键步骤。

在正常的减数分裂中，染色体首先复制，产生成对的姐妹染色单体。在该轮复制之后，进行两轮分裂，称为减数第一次分裂和减数第二次分裂。在减数第一次分裂期间，同源染色体重组并分离在两个细胞隔室(compartiment)中，这个两个细胞隔室各自都包含染色体的一个完整的单倍体内容物。在减数第二次分裂中，由减数第一次分裂产生的两组染色体进一步分裂，使姐妹染色单体分离开。因此，由该分裂产生的四个孢子是单倍体(n)，并且携带重组的遗传信息。

相比之下，在二倍体细胞的有丝分裂期间，染色体进行复制，姐妹染色单体分离产生子代细胞，该子代细胞是二倍体(2n)的并且在遗传学上与原始细胞相同。

已经示出，由减数分裂期间的异常现象得到的异常配子可用于几种感兴趣植物(包括作物)的遗传改良(供参考，参见例如，Ramanna&Jacobsen,Euphytica 2003,133,3-18)。具体地，2n配子和未减数分裂配子(apomeiotic gamete)可用于产生多倍体植物，或用于使不同倍性水平的植物，例如四倍体作物与其二倍体野生近缘种杂交，以便在植物育种方法中使用它们的遗传多样性。它们也可用于遗传作图的方法。未减数分裂配子对于产生无融合生殖植物(即能够从胚珠的母体组织形成种子的植物)也是感兴趣的，从而产生作为母本的遗传克隆的子代。

2n配子(也称为二倍体配子)是具有体细胞染色体数，而不是配子体染色体数的配子。引起2n配子形成的异常情况特别包括异常胞质分裂，第一次减数分裂或第二次减数分裂的遗漏(skip)，或纺锤体异形(供参考，参见V Veilleux,Plant Breeding Reviews,1985,3,252-288,or Bretagnolle&Thompson,New Phytologist,1995,129,1-22)。这些异常情况导致不同类别的未减数的配子。例如，第一次减数分裂的遗漏产生第一次分裂恢复(FDR)的配子，而第二次减数分裂的缺少产生第二次分裂恢复(SDR)的配子。已经报道了在各种植物物种中能够产生2n配子的许多突变体。然而，还未对这些植物中参与二倍体配子形成的突变进行表征。

未减数分裂配子是遗传学上与原始细胞相同的配子，保留亲本的所有遗传信息。未减数分裂配子的产生是无融合生殖的关键因素之一(Bicknell&Koltunow，Plant Cell，2004,16，S228-45)。虽然，超过400种植物是无融合生殖的，但这些植物中包括很少的作物物种。此外，通过杂交来引入这一性状的尝试已失败了(“The Flowering of Apomixis:From Mechanisms to Genetic Engineering”,2001；Editor:Savidan et al.；Publisher:CIMMYT,IRD,European Commission DG VI(FAIR),MEXICO,2001.Spillane et al.,Sexual Plant Reproduction,2001,14,179-187)。

迄今为止，已经证实了只有少数基因与2n或未减数分裂花粉的形成有关。

AtPS1(拟南芥平行纺锤体，Arabidopsis thaliana parallel spindles)的失活产生二倍雄孢子，从而产生具有重组遗传信息的可育的二倍花粉粒和在子代中产生自发三倍体植物(WO 2010/004431；d’Erfurth et al.,PLoS Genet.,2008,4,e1000274)。

TAM(缓慢异步的减数分裂，也称为CYCA1；2)或OSD1(第二次分裂的消失)的失活导致在减数第一次分裂之后过早地退出减数分裂，从而产生具有重组遗传信息的二倍体孢子和SDR配子(d’Erfuth et al.,PLoS Genet.,2010,6,e1000989；d’Erfuth et al.,PLoS Biol.,2009,7,e1000124；WO 2010/079432)。

SPO11-1编码植物中进行有效减数分裂重组所必需的蛋白，并且它的抑制消除重组和配对(Grelon et al.,Embo J.,2001,20,589-600)；而Rec8(At2g47980)编码着丝粒在减数分裂期间的单极取向所必需的蛋白(Chelysheva et al.,J.Cell.Sci.,2005,118,4621-32)，并且它的抑制改变染色单体的分离。Atspo11-1突变体经历不平衡的第一次分裂，随后进行第二次分裂，产生不平衡的孢子并且是不育的。Atspo11-1/Atrec8双突变体经历有丝分裂样分裂(而不经历正常的第一次减数分裂)，随后进行不平衡的第二次分裂，产生不平衡的孢子并且是不育的(Chelysheva et al.,J.Cell.Sci.,2005,118,4621-32)。

在osd1/spo11-1/rec8三重突变体中，spo11-1和rec8突变的存在导致有丝分裂样的第一次减数分裂，并且osd1突变的存在阻止发生第二次减数分裂。因此减数分裂完全替换成有丝分裂，且不会影响随后的有性生殖过程。因此，将osd1/spo11-1/rec8突变体命名为MiMe(有丝分裂而不是减数分裂)(d’Erfuth et al.,PLoS Biol.,2009,7,e1000124和WO 2010/079432)。由MiMe突变体获得的孢子和配子在遗传上与原始细胞相同。

迄今为止，能够产生2n配子或未减数分裂配子的植物的工程因此限于有限数目的基因。

因此，为了增加可以经修饰以产生高频的2n配子或未减数分裂配子的基因的数目，需要参与植物中的这些配子形成的其它基因。

TDM(三次分裂突变，THREE-DIVISION MUTANT)基因，也被命名为TDM1、MS5(蛋白雄性不育，PROTEIN MALE STERILE 5)或POLLENLESS 3编码属于植物中保守的小蛋白家族的蛋白。拟南芥(Arabidopsis thaliana)的TDM基因的序列能够在TAIR数据库中以登录号At4g20900来获得，或在GenBank数据库中以登录号NC_003075.7来获得。它编码434个氨基酸(aa)的蛋白，其序列在所附的序列表中表示为SEQ ID NO：1。

TDM基因被描述为在减数分裂结束时退出减数第二次分裂所需的基因。TDM突变导致多分体(polyad)的形成，以及由于在正常的减数第一次分裂和减数第二次分裂后进入异常的第三次减数分裂而引起的雄性不育。据表明，tdm突变体携带突变，该突变产生编码在其C末端缺少305或112个氨基酸的截短的TDM蛋白的基因(Bulankova et al.,The Plant Cell,2010,22,3791-3803；Cromer et al.,PLoS Genet.,2012,8,e10028652012；Glover et al.,The Plant Journal,1998,15,345-356；Ross et al.,Chrom.Res.,1997,5,551-559；WO/9730581)。

与此相比，如本文所示，本发明人已经确定了TDM基因中的显性突变，该显性突变使得在减数第二次分裂之前提前退出减数分裂，从而产生具有重组遗传信息的二倍体雄SDR配子和二倍体雌SDR配子以及二倍体孢子。此外，他们已经表明，在spo11-1 rec8双突变体的TDM基因中引入显性突变产生了MiMe突变体。

本发明人因此鉴定出了在植物中参与2n配子和未减数分裂配子形成的另一个基因。

与参与二倍体配子产生的其它突变相比(这些突变是隐性的，因此需要初生突变体(突变杂合)的额外自花授粉步骤来获得突变纯合的植物)，TDM中的显性的突变不需要这一步骤。携带TDM基因中的显性突变的初生突变体能够产生2n配子。

技术实现要素：

因此，本发明提供了一种用于获得产生第二次分裂恢复(SDR)2n配子的植物的方法，

其中，所述方法包括提供基因内包括显性突变的植物，所述基因在此指TDM基因，编码在此被指定为TDM蛋白的蛋白；

其中，当所述植物为芸苔属Brassica spp时，所述蛋白与SEQ ID NO:1的TDM蛋白的第1至286位氨基酸残基具有至少75％序列一致性；或者，当所述植物不同于芸苔属Brassica spp时，所述蛋白与所述残基具有至少30％序列一致性，并且所述蛋白的前60个氨基酸包括在此被指定为TPP/Q模体的模体X₁X₂X₃，其中，X₁为苏氨酸(T)，X₂为脯氨酸(P)，以及X₃为脯氨酸(P)或谷氨酰胺(Q)；以及

其中，所述显性突变使所述植物具有产生SDR 2n配子的能力。

在下面的描述中，使用标准的单字母氨基酸代码。

TDM基因和蛋白质序列能够在公共数据库中获得，诸如但不限于，Plaza数据库(http://bioinformatics.psb.ugent.be/plaza/)和phytozome网络门户http://www.phytozome.net/(phytosome v9.1)。

在使用具有默认参数的T-Coffee(v6.85)(http://toolkit.tuebingen.mpg.de/t_coffee)进行序列比对之后，对SEQ ID NO：1的第1至286位残基计算TDM蛋白的蛋白序列一致性；使用Bioedit 7.2.5由该比对获得百分比一致性(Hall,T.A.,1999.BioEdit:a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT.Nucl.Acids.Symp.Ser.41:95-98)。

每个物种具有一个或两个TDM基因，通常具有一个(图1和图2，以及表I)。取决于物种，TDM蛋白由约400个氨基酸至约850个氨基酸组成(图1)。根据图1中所示出各种被子植物物种的TDM蛋白的比对表明，TDM蛋白的前半部分是保守的。特别地，所有TDM蛋白的前60个氨基酸都包括保守的TPP/Q模体(图1)。

在使用具有默认参数的T-Coffee(v6.85)(http://toolkit.tuebingen.mpg.de/t_coffee)进行多重序列比对之后，计算各种被子物种的TDM蛋白与SEQ ID NO：1的TDM蛋白的第1至286位残基的百分比序列一致性。使用Bioedit 7.2.5由该比对获得一致性矩阵(Hall，TA，1999.BioEdit：a user-friendly biological sequence alignment editor and analysis program for Windows 95/98/NT.Nucl.Acids.Symp.Ser.41：95-98)。结果示于下表I中。

表I：TDM蛋白的序列一致性

*除了黄瓜Cucumus sativus、番茄Solanum lycopersicum、蓝花耧斗菜Aquilegia caerulea、菜豆Phaseolus vulgaris、柿树Prunus persica、雷蒙德氏棉Gossypium raimondii、大豆Glycine max和可可树Theobroma cacao来自于http://www.phytozome.net/(phytosome v9.1)之外，所有的序列都来自于Plaza数据库(http://bioinformatics.psb.ugent.be/plaza/)。

与SEQ ID NO：1的TDM蛋白的第1至286位氨基酸残基具有至少30％序列一致性的序列与SEQ ID NO：1的TDM蛋白的第1至286位氨基酸残基具有至少50％的序列相似性。因此，除了芸苔属Brassica spp.植物以外的其它植物的TDM蛋白可替代地被限定为与SEQ ID NO：1的TDM蛋白的第1至286位氨基酸残基具有至少50％的序列相似性，并且包括在该蛋白的前60个氨基酸中的TPP/Q模体。

根据本发明产生的SDR 2n配子可用于2n配子的所有常见应用中，例如用于生产多倍体植物，或允许不同倍性水平的植物之间进行杂交。它们也可用于遗传作图的方法，例如WO 2006/094774中所公开的“反向子代作图”的方法中。

根据用于获得产生第二次分裂恢复2n配子的植物的方法的优选实施方式，当所述植物不同于芸苔属Brassica spp时，所述蛋白与SEQ ID NO:1的TDM蛋白具有至少35％的序列一致性，并且逐渐优选地，具有至少40％、45％、50％、55％、60％、65％、70％、75％、80％、85％、90％、95％或98％的序列一致性；或者，当所述植物为芸苔属Brassica spp时，所述蛋白与SEQ ID NO:1的TDM蛋白具有至少80％的序列一致性，并且逐渐优选地，具有至少85％、90％、95％或98％的序列一致性。根据用于获得产生第二次分裂恢复2n配子的植物的方法的另一优选实施方式，所述TPP/Q模体位于所述蛋白的SEQ ID NO：1的第16～18位的区域中。

根据本发明，所述显性突变在TDM基因或TDM转基因的等位基因内，其中，所述TDM基因或转基因来自任何植物物种，例如表I中提及的那些物种。因此，能够产生SDR 2n配子的植物能够通过TDM基因的定向诱变或随机诱变来获得，或者通过遗传转化来获得。

根据本发明的用于获得产生第二次分裂恢复2n配子的植物的方法的优选实施方式，所述方法包括：

提供在TDM基因的等位基因内具有所述显性突变的植物，所述植物对于该突变是杂合的或纯合的。

TDM基因的诱变可以是定向的或随机的。在随机诱变(例如通过EMS诱变)之后，筛选期望基因内的突变体。用于高通量诱变和筛选的方法是本领域可知晓的。作为实例，可以提及TILLING(定向诱导基因组局部突变(Targeting Induced Local Lesions IN Genomes)，由McCallum et al.,Plant Physiology,2000,123,439-442所述)。利用标准技术进行定向诱变，该标准技术是本领域所已知的并且利用同源重组，优选与核酸酶例如TALEN或CRISPR组合。

根据本发明的用于获得产生第二次分裂恢复2n配子的植物的方法的另一优选实施方式，所述植物是转基因植物，并且所述方法包括：

a)用包含DNA构建体的载体转化至少一个植物细胞，所述DNA构建体包括具有所述显性突变的TDM基因；

b)培养所述转化的植物细胞，以使在其基因组中具有转基因的植物再生，该转基因包括所述DNA构建体。

DNA构建体包含TDM基因，该TDM基因可来自与其将引入的植物相同的物种，或来自不同的物种。

在TDM基因内的突变中，可以基于对于该突变是杂合的植物的表型性状，来确定导致产生SDR 2n配子的能力的那些突变：这些植物可以形成至少5％，优选至少10％，更优选至少20％，还更优选至少50％，并且高达100％的双孢体，作为减数分裂的产物。

可替代地，可通过它们恢复拟南芥(A.thaliana)spo11/rec8双突变体的可育力的能力，来确定TDM基因内，导致突变体或转基因植物具有产生SDR 2n配子的能力的显性突变，其中可育的spo11/rec8/tdm三重突变体对于TDM突变是杂合的，如本申请的实施例中所证实的，拟南芥(A.thaliana)spo11/rec8双突变体用于筛选显性突变，随后将该显性突变引入到感兴趣的植物中。

根据本发明的用于获得产生第二次分裂恢复2n配子的植物的方法的另一优选实施方式，所述显性突变包括保守TPP/Q模体的至少一个残基的突变或由保守TPP/Q模体的至少一个残基的突变组成。

上述突变可以是置换、插入或缺失，优选置换或缺失。

在更优选的实施方式中，所述显性突变包括T残基的突变和/或它相邻的P残基的突变，或由T残基的突变和/或它相邻的P残基的突变组成。TP是潜在的磷酸化位点。本申请的实施例证实了T16或P17残基的突变能够显著地提前退出减数分裂。不希望受理论的束缚，本发明人认为鉴于这些结果，TDM通过磷酸化来调节，以确保减数第一次分裂至减数第二次分裂的过渡。

因此，突变有利地是消除所述模体的T残基磷酸化的突变，即，破坏TP磷酸化位点的突变。

所述突变有利地是所述T残基和/或P残基被不同的残基置换，例如T被A置换，P被L置换。可替代地，所述突变是缺失T残基和P残基，并且最终缺失所述T残基和/或P残基两侧的额外残基，例如缺失1个至10个，优选1个至5个，甚至更优选1个或2个残基。

如上所限定，本发明的另一方面涉及包括TDM基因的DNA构建体，所述TDM基因具有使突变体/转基因植物具有产生SDR 2n配子的能力的显性突变。TDM基因可来自与其将引入的植物相同的物种，或来自不同的物种。DNA构建体包括可表达形式的TDM基因。优选地，TDM基因位于启动子的转录控制下，该启动子在植物细胞中有功能。启动子可以是TDM基因启动子，诸如所述TDM基因的内源启动子，或在植物中有功能的另一启动子。

在本领域中，具有大量供选择的适于在植物中表达异源基因的启动子。

它们可以例如获自植物、植物病毒或细菌(诸如农杆菌(Agrobacterium))。它们包括：组成型启动子，即在大多数组织和细胞中以及在大多数环境条件下具有活性的启动子；以及，组织特异性启动子或细胞特异性启动子，仅仅或主要在特定组织或特定细胞类型中具有活性；和，诱导型启动子，该诱导型启动子通过物理刺激或化学刺激，诸如由于线虫感染而产生的物理刺激或化学刺激而被激活。对启动子进行选择以便在性母细胞中具有功能。

常用于植物细胞中的组成型启动子的非限制性实例是花椰菜花叶病毒(CaMV)35S启动子、Nos启动子、1,5-二磷酸核酮糖羧化酶/加氧酶(rubisco)启动子、木薯叶脉花叶病毒(CsVMV)启动子。

可用于本发明的器官或组织特异性启动子特别包括能够赋予减数分裂相关表达的启动子，诸如DMC1启动子(Klimyuk&Jones，Plant J，1997,11,1-14)。

本发明的DNA构建体通常还包括转录终止子(例如，35S转录终止子、胭脂碱合酶(Nos)转录终止子或TDM基因终止子)。

本发明还包括重组载体，所述重组载体包含本发明的DNA构建体。通常，所述重组载体还包括一个或多个标记基因，允许对转化的宿主进行选择。

合适载体的选择，以及用于将DNA构建体插入到其中的方法是本领域普通技术人员所熟知的。载体的选择取决于预期的宿主以及所述宿主的预期的转化方法。在本领域中，对于许多植物物种，双子叶植物或单子叶植物，有多种用于植物细胞或植物的遗传转化方法。作为非限制性实例，可提及的是：病毒介导的转化，通过显微注射、电穿孔进行的转化，微粒介导的转化，农杆菌(Agrobacterium)介导的转化等。

本发明还提供了包括本发明的重组DNA构建体的宿主细胞。所述宿主细胞可以是：原核细胞，例如农杆菌(Agrobacterium)细胞；或者真核细胞，例如通过本发明的DNA构建体遗传转化的植物细胞。构建体可被瞬时表达；它也可被并入到稳定的染色体外复制子中，或整合到染色体中。

本发明人还发现，通过使TDM突变中的显性突变与两个基因的失活相组合，来得到产生未减数分裂配子的MiMe突变体，该两个基因中的一个基因对于减数分裂重组的起始是必需的并且选自spo11-1、SPO11-2、PRD1、PRD2(AT5G57880)、PRD3/PAIR1和DFO(AT1G07060)，另一个基因为REC8。

由MiMe突变体产生的未减数分裂配子可以与SDR 2n配子相同的方式，用于生产多倍体植物或用于不同倍性水平的植物的杂交。它们对于生产无融合生殖植物也是感兴趣的，即能够从胚珠的母体组织形成种子的植物，从而产生为母本的遗传克隆的子代。

因此，本发明的另一目的是用于获得产生未减数分裂配子的植物的方法，其中所述方法包括：

a)提供植物，所述植物包括如上定义的在TDM基因中的显性突变；

b)在所述植物中，抑制参与植物中起始减数分裂重组的第一蛋白，所述蛋白选自以下蛋白：

被指定为SPO11-1蛋白的蛋白，其中，所述蛋白与SPO11-1蛋白具有至少40％的序列一致性，并且逐渐优选地，具有至少45％、50％、55％、60％、65％、70％、75％、80％、85％、90％、95％或98％的序列一致性，或者所述蛋白与SPO11-1蛋白具有至少60％的序列相似性，并且逐渐优选地，具有至少65％、70％、75％、80％、85％、90％、95％或98％的序列相似性，所述SPO11-1蛋白在SwissProt数据库中的序列登录号为Q9M4A2，对应于所附序列表中的SEQ ID NO:29；

被指定为SPO11-2蛋白的蛋白，其中，所述蛋白与SPO11-2蛋白具有至少40％的序列一致性，并且逐渐优选地，具有至少45％、50％、55％、60％、65％、70％、75％、80％、85％、90％、95％或98％的序列一致性，或者所述蛋白与SPO11-2蛋白具有至少60％的序列相似性，并且逐渐优选地，具有至少65％、70％、75％、80％、85％、90％、95％或98％的序列相似性，所述SPO11-2蛋白在SwissProt数据库中的序列登录号为Q9M4A2，对应于所附序列表中的SEQ ID NO:30；

被指定为PRD1蛋白的蛋白，其中，所述蛋白与PRD1蛋白具有至少25％的序列一致性，并且逐渐优选地，具有至少30％、35％、40％、45％、50％、55％、60％、65％、70％、75％、80％、85％、90％、95％或98％的序列一致性，或者所述蛋白与PRD1蛋白具有至少35％的序列相似性，并且逐渐优选地，具有至少40％、45％、50％、55％、60％、65％、70％、75％、80％、85％、90％、95％或98％的序列相似性，所述PRD1蛋白在GenBank数据库中的序列登录号为ABQ12642，对应于所附序列表中的SEQ ID NO:31；

被指定为PRD2蛋白的蛋白，其中，所述蛋白与PRD2蛋白具有至少25％的序列一致性，并且逐渐优选地，具有至少30％、35％、40％、45％、50％、55％、60％、65％、70％、75％、80％、85％、90％、95％或98％的序列一致性，或者所述蛋白与PRD2蛋白具有至少35％的序列相似性，并且逐渐优选地，具有至少40％、45％、50％、55％、60％、65％、70％、75％、80％、85％、90％、95％或98％的序列相似性，所述PRD2蛋白在Plaza数据库中的序列登录号为AT5G57880，对应于所附序列表中的SEQ ID NO:32；

被指定为PAIR1蛋白的蛋白，其中，所述蛋白与PAIR1蛋白具有至少30％的序列一致性，并且逐渐优选地，具有至少35％、40％、45％、50％、55％、60％、65％、70％、75％、80％、85％、90％、95％或98％的序列一致性，或者所述蛋白与PAIR1蛋白具有至少40％的序列相似性，并且逐渐优选地，具有至少45％、50％、55％、60％、65％、70％、75％、80％、85％、90％、95％或98％的序列相似性，所述PAIR1蛋白在GenBank数据库中的序列登录号为NP_171675，对应于所附序列表中的SEQ ID NO:33；

被指定为DFO蛋白的蛋白，其中，所述蛋白与DFO蛋白具有至少30％的序列一致性，并且逐渐优选地，具有至少35％、40％、45％、50％、55％、60％、65％、70％、75％、80％、85％、90％、95％或98％的序列一致性，或者所述蛋白与DFO蛋白具有至少40％的序列相似性，并且逐渐优选地，具有至少45％、50％、55％、60％、65％、70％、75％、80％、85％、90％、95％或98％的序列相似性，所述DFO蛋白在Plaza数据库中的序列登录号为AT1G07060，对应于所附序列表中的SEQ ID NO:34；以及

c)在所述植物中，抑制被指定为REC8蛋白的第二蛋白，其中，所述蛋白与REC8蛋白具有至少40％的序列一致性，并且逐渐优选地，具有至少45％、50％、55％、60％、65％、70％、75％、80％、85％、90％、95％或98％的序列一致性，或者所述蛋白与REC8蛋白具有至少45％的序列相似性，并且逐渐优选地，具有至少50％、55％、60％、65％、70％、75％、80％、85％、90％、95％或98％的序列相似性，所述REC8蛋白在GenBank数据库中的序列登录号为NP_196168，对应于所附序列表中的SEQ ID NO:35。

使用在默认参数下的BLASTP程序，计算spo11-1、spo11-2、prd1、PRD2、DFO、PAIR1或REC8蛋白的在此所提供的蛋白序列一致性和相似性的值。使用评分矩阵BLOSUM62，进行相似性计算。

可通过消除、阻断或降低其功能，或者有利地通过阻止或下调相应基因的表达，来获得对上述SPO11-1、SPO11-2、PRD1、PRD2、PAIR1、DFO或Rec8蛋白的抑制。在申请WO2010/00431中详细公开了SPO11-1、SPO11-2、PRD1、PRD2、PAIR1、DFO或Rec8蛋白以及所述蛋白的抑制。

例如，所述蛋白的抑制可以通过诱变相应的基因或其启动子，并选择所述蛋白的活性部分或完全丧失的突变体来获得。所述抑制公开在WO 2010/00431的第5页的最后一段开始至第6页的第4段结束，以及第6页的最后一段，这些内容通过引用并入本文中。

可替代地，通过使相应的基因沉默来获得目标蛋白的抑制。这种抑制公开在WO2010/00431的第7页第4段开始至第9页的倒数第二段结束，以及第10页的前三段，这些内容通过引用并入本文中。

根据本发明的用于获得能够产生未减数分裂配子的植物的方法的优选实施方式，所述方法包括：

a)提供在TDM基因的等位基因内具有显性突变，从而具有产生SDR 2n配子的能力的植物，该植物对于该突变是杂合的；

b)提供在选自SPO11-1、SPO11-2、PRD1、PRD2、DFO或PAIR1基因的基因的等位基因内具有突变，从而抑制由所述等位基因编码的蛋白的植物，所述植物对于该突变是杂合的；

c)提供在REC8基因的等位基因内具有突变，从而抑制由所述等位基因编码的蛋白的植物，所述植物对于该突变是杂合的；

e)使步骤a)、步骤b)和步骤c)的植物杂交，以获得在TDM基因的等位基因内具有显性突变，在选自SPO11-1、SPO11-2、PRD1、PRD2、DFO或PAIR1基因的基因的等位基因内具有突变，以及在REC8基因的等位基因内具有突变的植物，所述植物对于每一突变都是杂合的；

f)使步骤e)的植物自花授粉，以获得对于TDM基因内的突变是纯合的，对于选自SPO11-1、SPO11-2、PRD1、PRD2、DFO或PAIR1基因的基因内的突变是纯合的，以及对于REC8基因内的突变的是纯合的植物。

根据本发明的用于获得能够产生未减数分裂配子的植物的方法的另一优选实施方式，所述植物是转基因植物，并且所述方法包括：

a)用含有本发明的包含如上定义的TDM基因中的显性突变的DNA构建体的载体，含有靶向选自SPO11-1、SPO11-2、PRD1、PRD2、DFO或PAIR1的基因的DNA构建体的载体，以及含有靶向REC8基因的DNA构建体的载体转化至少一个植物细胞；

b)培养所述转化的植物细胞，以使在它的基因组中具有含所述DNA构建体的转基因的植物再生。

在TDM基因中包括显性突变的DNA构建体的表达，为所述转基因植物提供了产生2n SDR配子的能力。包括TDM基因中的显性突变的DNA构建体基因，靶向选自SPO11-1、SPO11-2、PRD1、PRD2、DFO或PAIR1的基因的DNA构建体，以及靶向REC8基因的DNA构建体的共表达，使由这三种基因编码的蛋白下调，并且为所述转基因植物提供产生未减数分裂配子的能力。

本发明还涵盖能够通过本发明的方法获得的能够产生SDR 2n配子或未减数分裂配子的植物。

这特别包括如上定义的在TDM基因内包含显性突变的植物，以及进一步包括第一突变和第二突变的植物，所述第一突变在选自SPO11-1、SPO11-2、PRD1、PRD2、DFO或PAIR1基因的基因内，其中由所述基因编码的SPO11-1、SPO11-2、PRD1、PRD2、DFO或PAIR1蛋白因该突变而被抑制，所述第二突变在REC8基因内，其中REC8蛋白因该突变而被抑制。

这还包括由本发明的至少一种DNA构建体遗传转化的植物。优选地，所述植物是转基因植物，其中所述构建体被包含在整合到植物基因组中的转基因中，从而使它被传递给连续的植物后代中。

本发明还涵盖用于产生SDR 2n配子的方法，其中所述方法包括培养能够通过本发明的方法获得的植物并回收由所述植物产生的配子。优选地，所述配子包括至少10％、更优选至少20％，并且逐渐优选地，至少30％、40％、50％、60％、70％、80％或90％的可育2n配子。

本发明还还涵盖用于产生未减数分裂配子的方法，其中所述方法包括培养能够通过本发明的方法获得的植物并回收由所述植物产生的配子。优选地，所述配子包括至少10％、更优选至少20％，并且逐渐优选地，至少30％、40％、50％、60％、70％、80％或90％的可育未减数分裂配子。

本发明适用于农艺学上感兴趣的范围广泛的单子叶植物或双子叶植物。作为非限制性实例，可提及的是土豆、稻、小麦、玉米、番茄、黄瓜、苜蓿、甘蔗、甘薯、木薯、三叶草、大豆、射线草、香蕉、甜瓜、西瓜、棉，或观赏植物(诸如玫瑰、百合、郁金香和水仙)。

除非另有说明，否则本发明的实施将采用本领域技术范围内的常规技术。这样的技术在文献中有充分的解释。

附图说明

除了上述设置之外，本发明还包括其他设置，这些其他设置，将在下文中的描述中显现出来，并且参照所附附图，指本发明主题的示例性实施方式，在附图中：

图1示出了来自多种被子植物物种的TDM蛋白的比对。使用具有默认参数的T-Coffee(v6.85)对序列进行比对(http://toolkit.tuebingen.mpg.de/t_coffee)。序列比对用BioEdit编辑。仅示出了在TDM蛋白中保守的序列的前半部分。在比对的TDM蛋白中显示出80％以上一致性的残基用阴影表示。包括TPP/Q模体的保守区则加边框来表示。

图2示出了来自多种被子植物的TDM蛋白、来自十字花目的TDM_样1蛋白以及来自拟南芥(Arabidopsis thaliana)和二穗短柄草(Brachypodium distachyon)的TDM样蛋白的系统树。该分析在Phylogeny.fr平台上进行并包括以下步骤。使用以下成对比对方法，利用T-Coffee(v6.85)来进行序列比对：10次最佳局部比对(Lalign_pair)，精确的全局比对(slow_pair)。在比对之后，从比对中去除具有缺口的位置。利用在PhyML程序(v3.0aLRT)中实施的最大似然法来重建系统树。示出了所有物种中的TDM进化枝的蛋白(包括在十字花目中的TDM-样1)。仅示出了拟南芥(Arabidopsis thaliana)和二穗短柄草(Brachypodium distachyon)的更远的TDM旁系同源。

At：拟南芥Arabidopsis thaliana，A1：深山南芥Arabidopsis lyrata，Bra：芸苔Brassica rapa，Sly：番茄Solanum lycopersicum，St：马铃薯Solanum tuberosum，Csa：黄瓜Cucumis sativus，Eucgr：桉树Eucalyptus grandis，Cp：番木瓜Carica papaya，ME：木薯Manihot esculenta，TC：可可树Theobroma cacao，Goraii：雷蒙德氏棉Gossypium raimondii，FV：野草莓Fragaria vesca，Pp：柿树Prunus persica，LJ：莲藕Lotus japonicus，MT：蒺藜苜蓿Medicago truncatula，GM：大豆Glycine max，Pv：芸豆Phaseolus vulgaris，VV：菜豆Phaseolus vulgaris，Aq：蓝花耧斗菜Aquilegia caerula，OS：粳稻Oryza sativa japonica，OSINDICA：籼稻Oryza sativa indica，BD二穗短柄草Brachypodium distachyon，SB：高粱Sorghum bicolor，ZM：玉米Zea mays，Si：粟Setaria italica。

图3显示spo11-1 rec8(s)-40突变体产生二分体并且是四倍体的。(A～C)。用甲苯胺蓝染色的雄性减数分裂产物。(A)野生型产生孢子的四分体。(B)spo11-1 rec8产生孢子的不平衡的多分体。(C)spo11-1 rec8-40产生孢子的二分体。(D～F)有丝分裂的核型。(D)野生型是二倍体，具有排列在有丝分裂中期板上的10个染色体。(E)spo11-1 rec8是二倍体。(F)spo11-1 rec8(s)-40是四倍体，具有排列在在有丝分裂中期板上的20个染色体。比例尺＝10μM。

图4示出了TDM-P17L、TDM-T16A和TDM-Δ14-19的减数分裂产物。(A)用TDM-P17L转化的spo11-1 rec8突变体。由(B)TDM-P17L、(C)TDM-T16A或(D)TDM-Δ14-19转化的野生型植物。与野生型中的四分体(图3A)和spo11-1 rec8中的多分体(图3B)相比，观察到孢子的二分体。

图5示出了在野生型、spo11-1 rec8、TDM-P17和spo11-1 rec8 TDM-P17植物中的减数分裂的染色体分散。(A至D)野生型的减数分裂。(A)五个二价体在中期I时对齐，和(B)成对的同源染色在末期I时分布至两个核中。(C)五对姐妹染色单体在两个中期板上对齐。(D)在末期II，形成四个平衡的核。(E至H)spo11-1 rec8中的减数分裂。第一次分裂类似于有丝分裂，其中，(E)10对染色单体对齐在中期板上，并且(F)分离成两组10个染色单体。(G)在中期II，单个染色单体未能适当地对齐，导致(H)末期II时产生可变数目的不平衡的核。(I至J)用TDM-P17转化的野生型植物中的减数分裂。观察到单次减数第一次分裂样的分裂。(K至L)用TDM-P17转化的spo11-1 rec8植物中的减数分裂。观察到单次有丝分裂样的分裂。

具体实施方式

实施例

实验步骤

1.生长条件和基因分型

在21℃下，在16小时至18小时光周期和70％相对湿度下，如先前所述在温室中(Vignard et al.,PLoS Genet.,2007,3,1894-1906)或者如前所述离体在拟南芥Arabidopsis培养基上(Estelle and Somerville,Mol.Genet.,1987,206,200-206)上培养Arabidopsis拟南芥植物。

如先前所述(d’Erfurth et al.,PLoS Biol.,2009,7,e1000124)对spo11-1-3rec8-2植物进行基因分型。如Cromer et al.,PLoS Genet.,2012,8,e1002865所述对tdm-3植物进行基因分型。

2.EMS诱变和突变鉴定

如先前所述(Crismani et al.,Science,2012,336,1588-1590)进行EMS诱变。通过HigSeq^TM2000(Illumina)进行全基因组测序。与拟南芥Arabidopsis thaliana TAIR10(哥伦比亚栽培品种)的对照基因组相比，生成SNP列表。

3.细胞学和倍性分析

使用由d'Erfurth等人(PLoS Genet.,2008,4,e1000274)描述的技术，对雄性减数分裂产物进行观察、染色体分散和倍性测量。

4.定向诱变构建体和植物转化

使用TDM U(5'-GACATCGGCACTTGCTTAGAG-3'；SEQ ID NO：36)和TDM L(5'-GCGATATAGCTCCCACTGGTT-3'；SEQ ID NO：37)通过PCR扩增TDM基因组片段。扩增覆盖ATG前的986个核苷酸和终止密码子后的537个核苷酸。通过Gateway^TM技术(Invitrogen)，将PCR产物克隆到pDONR207^TM载体(Invitrogen)中，以产生pENTR-TDM；使用Stratagene QuickChange^TM定点诱变试剂盒，根据制造商的说明对pENTR-TDM进行定向诱变。用于产生TDM的突变形式的诱变引物为SEQ ID NO：38至SEQ ID NO：41：

-TDM-P17L:5′-GAGTTTACTATACTCTGCCGCCGGCGAGAAC-3′；

-TDM-T16A:5′-CTCCACCTGGAGTTTACTATGCCCCGCCGCCGGCGAGA-3′；

-TDM-Y14A:5’-CCACCTGGAGTTGCGTATACTCCGCCGCGGCG-3′；

-TDM-Δ14-19:5’-CCACCTGGAGTTGCGAGAACAAGTGATCATGTGGC-3’；

以及它们各自的反向互补引物。为了产生用于植物转化的双元载体，使用Gateway ^TM系统的二元载体pGWB1进行LR重组反应(Nakagawa et al.,Journal of Bioscience and Bioengineering.2007,104,34-41)。对野生型植物以及分离成spo11-1或rec8或tdm-3突变的植物群，使用农杆菌介导的花浸渍法(Clough,S.J.and Bent A.F.,Plant J.,1998,16,735-743)转化得到的二元载体pTDM，pTDM-P17L，pT16A和pTDM-Y14A。在含有20mg/L潮霉素的琼脂板上对转化的植物进行选择。

实施例1：TDM中的显性突变导致提早退出减数分裂

为了鉴定控制减数分裂进程的新基因，基于以下观点来设计遗传筛选：导致遗漏第二次减数分裂的突变，诸如osd1和cyca1；2/tam将恢复仅在第二次减数分裂中具有不平衡的染色体分离缺陷的突变体的可育力(d’Erfurth et al.,PLoS Biol.,2009,7,e1000124；d’Erfurth et al.,PLoS Genet.,2010,6,e1000989和WO 2010/079432)。这是spo11rec8双突变体的情况，其中第一次减数分裂类似于有丝分裂(姐妹染色单体平衡分离至相对极)，但是第二次分裂是不平衡的且产生非整倍体的配子，因此具有非常有限的可育力(图5)(Chelysheva et al.,J.Cell.Sci.,2005,118,4621-32)。在OSD1(d’Erfurth et al.,PLoS Biol.,2009,7,e1000124 2009)或CYCA1；2/TAM(d’Erfurth et al.,PLoS Genet.,2010,6,e1000989)中的导致在减数第二次分裂前退出减数分裂的突变，确实能够恢复spo11-1 rec8的可育力。因此，基于spo11-1 rec8的可育力的恢复来运行遗传筛选，旨在鉴定出赋予比osd1或tam还相似缺陷的突变体。尽管它们具有减数分裂分离的缺陷，spo11-1 rec8植物产生了经甲基磺酸乙酯(EMS)诱变的足够的剩余种子。假定对EMS突变是杂合的M1植物进行自花授粉，并且在5个批次中收获以产生M2家族。筛选了与spo11-1 rec8非诱变对照相比，可育力增加的约2000个M2家族(400个批次)。

三个批次分离具有增加可育力的植物。基因分型证实，它们是spo11-1 rec8突变体，这表明了其是真正的抑制因子。通过甲苯胺蓝染色对雄性减数分裂产物进行的分析示出，在所有三种情况下，可育植物产生孢子的二分体(如在osd1或cyca1；2/tam中所观察到的)，而不是四分体(如在野生型中所观察到的)，表明了在这些植物中并没有发生第二次减数分裂(图3)。候选基因(CYCA1；2/TAM和OSD1)的测序确定了CYCA1；2/TAM在三个家族中的两个中的隐性突变。所确定的突变是外显子7(TAIR10chr1：29082522C>T)中的剪接位点，和5'UTR区域中的引入上游框外起始密码子(TAIR10chr1：29084174G>A)的突变。互补试验表明它们是等位基因，证实了CYCA1；2中的突变引起二分体表型和可育力的恢复。第三个家族(spo11rec8(s)-40)在OSD1和CYCA1；2中没有突变，是本研究的重点。

出乎意料地，染色体分散表明四种植物是四倍体的(图3)。这表明因果突变(causal mutation)是显性的，并且导致在M1植物的雄性和雌性器官中均产生二倍体配子。与野生型相比，以～100X覆盖度对两种姐妹植物的大部分所进行全基因组测序显示出1144个SNP的存在。然而，仅15个SNP呈现为纯合子。这些少数纯合子SNP分散在基因组中，表明它们在诱变前就存在于spo11-1rec8株系中，而不是由于EMS诱导的突变所固定的。几乎所有检测到的突变都是杂合子这一事实进一步表明因果突变是显性的。该突变会在M1植物中显型表达，在与spo11-1 rec8突变组合下，导致二倍体克隆配子的产生(如在spo11-1rec8osd1三重突变体中观察到的)(MiMe,d’Erfurth et al.,PLoS Biol.,2009,7,e1000124和WO 2010/079432)，因此四倍体M2植物中维持了来自M1植物的EMS诱导的突变的杂合性。

然后，在杂合子SNP中寻找候选的因果突变。在这1129个突变中，预测341个突变将影响编码序列(无义、错义或剪接位点)。其中，TDM中导致氨基酸变化的突变(TDM-P17L)由于潜在的因果显性突变而成为良好候选者。之前已证实，TDM对于减数第二次分裂结束时退出减数分裂是必需的。即使在tdm敲除突变体中观察到的减数分裂缺陷(额外的一轮分裂)与(spo11rec8(s)-40)缺陷显著不同，TDM中的显性突变仍然是(spo11rec8(s)-40)中的因果突变的潜在候选者。

为了验证该假设，产生含有TDM基因(包括启动子和终止子)的基因组克隆，并使其突变以重新产生在筛选中所鉴定的突变(TDM-P17L)，其中TDM基因能够补充tdm-3突变体(n＝8个转化体，100％四分体)。当引入spo11-1rec8植物中时，TDM-P17L克隆恢复了初代转化体的可育力(n＝2/3，spo11-1 rec8：0.1个种子/果实(n＝197)，spo11-1 rec8 TDM-P17L#15：25个种子/果实(n＝15)，spo11-1 rec8 TDM-P17L#67：48个种子/果实(n＝10))，并产生二分体(图4，表II)。这表明TDM中的突变确实是spo11rec8(s)-40中的因果显性突变。对spo11-1rec8TDM-P17L转化体中减数分裂染色体分散的分析显示出，有丝分裂样的第一次分裂，其中10个单价体在中期I时对齐，并且姊妹染色单体在后期I分离，且不进行第二次分裂(图5)。接下来，研究了spo11-1rec8 TDM-P17L后代的倍性水平。在自交子代中，仅发现四倍体(4n)(表III)。当spo11-1rec8 TDM-P17L花粉用于使野生型植物受精时，所有得到的子代都是三倍体(表III)。当spo11-1 rec8 TDM-P17L胚珠经野生型花粉粒受精时，仅发现三倍体植物(表III)。这证实了被TDM-P17L转化的spo11-1 rec8突变体产生高水平的雄性和雌性(100％)有丝分裂样来源的孢子，其导致功能性二倍体配子。

当引入到野生型植物和tdm-3突变体中时，TDM-P17L基因组克隆通过产生二分体来修饰两种基因型的减数分裂表型(图4，表II)。

表II：初代转化体的减数分裂产物

产生二分体的TDM-P17L植物显示出野生型第一次分裂并且不发生减数第二次分裂(图5)，从而形成2n配子，以及来自osd1和cyca1；2/tam之一的表型重现(d’Erfurth et al.,PLoS Biol.,2009,7,e1000124和WO 2010/079432；d’Erfurth et al.,PLoS Genet.,2010,6,e1000989)。在TDM-P17L植物的后代中测量倍性水平(表III)。在自交子代中，发现了四倍体和三倍体。当TDM-P17L胚珠经野生型花粉粒受精时，分离出二倍体植物和三倍体植物(表III)。

表III：spo11-1 rec8 TDM-P17L和TDM-P17L后代的倍性

总之，tdm-p17L显性突变赋予了与隐性osd1或tam突变类似的减数分裂缺陷，使在第二次分裂之前提前退出减数分裂，从而产生二倍体的雄性和雌性配子。

TDM属于植物中保守的小家族蛋白。例如，拟南芥Arabidopsis基因组含有与TDM显示出显著序列相似性的5个其他基因(图2)。这些类TDM基因的功能是未知的。蛋白序列的分析表明，因果突变在仅TDM蛋白亚家族中保守的小结构域中，所述TDM蛋白亚家族通常每个植物物种含有一个或两个基因(图1)。Pro17氨基酸以及相邻的Thr16氨基酸是绝对保守的(图1)。这就限定了T16上的最小共有磷酸化位点。为了对此进行验证，通过用不可磷酸化的氨基酸(TDM-T16A)来置换可磷酸化的氨基酸，并通过删除整个保守结构域(TDM-Δ14_19)来建立基因组TDM基因的两个其它潜在的磷酸化丢失形式。当引入到野生型植物中时，TDM-T16A和TDM-Δ14_19都以显性方式产生了二分体表型，重现了TDM-P17L的作用(表II；图4)。此外，当引入到tdm-3突变体时，TDM-Δ14_19也显示二分体表型(表II)。然而，当引入到野生型时，TDM酪氨酸14(TDM-Y14A)(结构域的保守性稍低的氨基酸)的突变不能产生二分体表型，但能够补充tdm-3突变(表II)。总之，TDM-P17L、TDM-T16A和TDM-Δ14-19突变的表达同样能够显性地提早退出减数分裂。由于TP是潜在的磷酸化位点，这个结果表明TDM可能受到磷酸化的调节以确保减数第一次分裂至减数第二次分裂的过渡。

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Lys His Val Gln Leu Ile Asp Lys Asp Pro Ser Lys Ala Ile Ser Leu

65 70 75 80

Phe Trp Ala Ala Ile Asn Ala Gly Asp Arg Val Asp Ser Ala Leu Lys

85 90 95

Asp Met Ala Val Val Met Lys Gln Leu Asn Arg Ser Asp Glu Ala Ile

100 105 110

Glu Ala Ile Lys Ser Phe Arg His Leu Cys Pro His Asp Ser Gln Glu

115 120 125

Ser Leu Asp Asn Val Leu Val Glu Leu Tyr Lys Arg Ser Gly Arg Val

130 135 140

Glu Glu Glu Ile Glu Met Leu Gln Asn Lys Leu Arg Asn Ile Glu Gly

145 150 155 160

Gly Thr Val Phe Gly Gly Lys Arg Thr Lys Ile Ala Arg Ser Gln Gly

165 170 175

Lys Lys Ile Gln Ile Thr Ile Glu Gln Glu Lys Ser Arg Ile Leu Gly

180 185 190

Asn Leu Ala Trp Ala Tyr Leu Gln Gln His Asn Tyr Gly Ile Ala Glu

195 200 205

Gln His Tyr Arg Lys Ala Leu Ser Leu Glu Pro Asp Met Asn Lys Gln

210 215 220

Cys Asn Leu Ala Ile Cys Leu Met His Met Asn Arg Ile Ser Glu Ala

225 230 235 240

Lys Ser Leu Leu Gln Asp Val Lys Ala Ser Ser Gly Asn Glu Gln Met

245 250 255

Asp Glu Ser His Ser Lys Ser Tyr Glu Arg Ala Leu Asp Met Leu Ile

260 265 270

Gln Val Glu Ser Gln Ser Met Leu Glu Pro Val Ala Lys Glu Pro Asp

275 280 285

Lys Gly Arg Glu Ile Gln Arg Pro Ser Thr Pro Cys Arg Asp Arg Gly

290 295 300

Leu Lys Glu Ala Gly Ile Phe Leu Pro Arg Asn Glu Asp Asn Ile Ser

305 310 315 320

Gly Phe Met Gly Arg Arg Arg Leu Pro Tyr Ala His Trp Glu Gly Lys

325 330 335

Met Leu Ile Asp Glu Gln Asn Gly Glu Ser Tyr Arg Arg Asn Pro Leu

340 345 350

Glu Lys Asn Asp Asn Phe Pro Gly Tyr Asp Asp Arg Ser Ser Lys Cys

355 360 365

Thr Pro Ile Gly Gln Lys Gly Tyr Leu Gln Ser Ser Pro Gln Ser Met

370 375 380

Phe Thr Glu Lys Trp Arg Ile Gly Ser Tyr Trp Glu Ser Pro Cys Glu

385 390 395 400

Gly Tyr Ser Thr Gly Glu Glu Val Gly Ser Ala Gln Lys Lys Ile Tyr

405 410 415

Ala Ser Ser Ala Ala Ser Lys Lys Asn Ser Glu Ala Leu Phe Thr Gln

420 425 430

Pro Arg Arg Cys Ser Gln Gly Phe Asn Asn Ala Asp Gln Lys Arg Gly

435 440 445

Gly Arg Trp Gly Glu Asp Thr Val Arg Asn Ser Ile Arg Lys Leu Ser

450 455 460

Phe Glu Gln Ser Leu Thr Ser Glu Ser Glu Pro Leu His Ser Ile Gln

465 470 475 480

Asn Leu Asn Glu Lys Pro Gln Ala Ser Asn Asn Gly Lys Ser Glu Asn

485 490 495

Ser Ala Thr Gly Pro Val Glu Glu Glu Val Gln Glu Gly Leu Ser Gly

500 505 510

Val Leu Phe Thr Gln Pro Arg Asn Ser Leu Leu Trp Leu Asn Asn Arg

515 520 525

Asp Gln Arg Met Glu Arg Trp Ala Glu Glu Ser Val Gly Cys Pro Phe

530 535 540

Arg Lys Leu Ser Phe Glu Lys Asn Ile Thr Gly Val Thr Pro His Ser

545 550 555 560

Ala Asp Gly Leu Asn Gly Glu Pro Leu Phe Ser Ser Lys Asp Glu Ser

565 570 575

Glu Ile Gly Leu Glu Arg Pro Ala Asn Ala Pro Asn Lys Lys Ser Trp

580 585 590

Ala Asp Met Val Glu Glu Glu Glu Lys Glu Glu Leu Leu Asn Ser Tyr

595 600 605

Asp Gly Phe Asn Arg Glu Glu Val Phe Asn Asp Glu Asn Leu Asn Ser

610 615 620

Asn Ile Ile Tyr Pro Arg Pro Asp Cys Lys Asp His Ile Gly Asn Ile

625 630 635 640

Thr Gln Gln Leu Glu Ser Phe Asp Met Lys Gly Gly Asp Asn Ala Ser

645 650 655

Ala Asn Thr Val Ser Ser Arg Arg Asn Arg Leu Pro Val Phe Arg Asp

660 665 670

Ile Thr Ser Ser

675

<210> 6

<211> 631

<212> PRT

<213> 木薯（Manihot esculenta）

<400> 6

Met Trp Ser His His Glu Asn Asn Ile Pro Ala Arg Gly Phe Ser Thr

1 5 10 15

Pro Pro Pro Ser Trp Lys Ser Pro His Cys Leu Ala Ala Met Pro Met

20 25 30

Ser Glu Arg Lys Ser Val Ser Ser Gly Cys Lys Arg Asp Leu Phe His

35 40 45

Val Ile His Lys Val Pro Ala Gly Asp Ser Pro Tyr Val Arg Ala Lys

50 55 60

His Val Gln Leu Ile Glu Lys Asp Pro Ser Arg Ala Ile Ser Leu Phe

65 70 75 80

Trp Ala Ala Ile Asn Ala Gly Asp Arg Ile Asp Ser Ala Leu Lys Asp

85 90 95

Met Ala Val Val Met Lys Gln Leu Asn Arg Ser Asp Glu Ala Ile Glu

100 105 110

Ala Ile Lys Ser Phe Arg Arg Leu Cys Pro Tyr Asp Ser Gln Glu Ser

115 120 125

Ile Asp Asn Val Leu Val Glu Leu Tyr Lys Arg Ser Gly Arg Ile Glu

130 135 140

Glu Glu Ile Glu Met Leu His Leu Lys Leu Lys Asn Ile Glu Glu Gly

145 150 155 160

Ile Ala Phe Gly Gly Lys Arg Thr Lys Thr Ala Arg Ser Gln Gly Lys

165 170 175

Lys Ile Gln Ile Thr Val Glu Gln Glu Arg Ser Arg Ile Leu Gly Asn

180 185 190

Leu Ala Trp Ala Tyr Leu Gln His His Asp Tyr Gly Leu Ala Glu Gln

195 200 205

Tyr Tyr Arg Lys Ala Leu Ser Leu Glu Pro Asp Lys Asn Lys Gln Cys

210 215 220

Asn Leu Ala Ile Cys Leu Met His Met Asn Arg Ile Pro Glu Ala Lys

225 230 235 240

Ser Leu Leu Gln Ala Val Ser Asp Ser Cys Gly Ser Lys Gln Met Asp

245 250 255

Asp Ser Tyr Ala Lys Ser Phe Glu Arg Ala Val Asp Ile Leu Thr Glu

260 265 270

Leu Glu Ser Lys Ser Val Leu Lys Pro Ala Glu Glu Asp Lys Glu Asn

275 280 285

Gln Arg Ser Leu Ala Ser Pro Leu Leu Ser Asp Lys His Asp Arg Gly

290 295 300

Ser Tyr Cys Gly Ile His Ser Glu Glu Gln Gly Asn Phe Ile Ser Ser

305 310 315 320

Ser Gly Lys Gly Pro Gly Leu Ala Asn Lys Arg Met Leu Asp Ser Pro

325 330 335

Ala Ala Val Leu Tyr Thr Gln Pro Lys Arg Val Met Gly Arg Ser Asp

340 345 350

Glu Glu Glu Gln Arg Arg Gly Val Gly Trp Glu Asn Asp Thr Val Glu

355 360 365

Lys Pro Ser Lys Asn Val Ser Ala Cys Ile Ile Arg Ser Leu Asp Gly

370 375 380

Glu Leu Leu Asp Pro Pro Ala Glu Arg Asn Trp Arg Glu Lys Ser Trp

385 390 395 400

Thr Glu Val Ala Gln Gly Lys Ile Thr Gly Val Thr Val Pro Tyr Gln

405 410 415

Phe Ser Gln Pro Arg Ile Arg Ala Phe Thr Gly Tyr Asn Asp Ala His

420 425 430

Leu Lys Asp Glu Asn Val Thr Lys Ser Asp Ser Gln Gln Pro Ser Trp

435 440 445

Arg Ser Asn Ala Arg Glu Thr Gly Gly Gln Met Gly Ser Thr Asn Glu

450 455 460

Lys Ser Asp Ala Ser Ser Lys Phe His Leu Glu Gln Asn Met Val Val

465 470 475 480

Asp Asp Ala Arg Gln Ser Glu Thr Ser Ile Asp Gly Lys Cys Gly Gln

485 490 495

Thr Phe Gly Ile Asn Gly Cys Leu Gly Lys Asn Ser Ser Ser Lys Phe

500 505 510

Ala Ile Lys Ser Trp Ala Asp Met Val Glu Glu Glu Glu Glu Lys Leu

515 520 525

Leu Thr Gly Lys Asp Leu Ser Pro Tyr Phe Asp Gly Gly Trp Asp Tyr

530 535 540

Glu Glu Glu Ser Ala Asp Glu Asn Gln Asp Ser Asn Ile Ile His Gln

545 550 555 560

Thr Ser Cys Pro Lys Ser Pro Ala Glu Ala Ile Asn Gln Lys Phe Glu

565 570 575

Ala Phe Asp Leu Lys Asp Gly Phe Ala Ala Phe Ser Asn Ala Val Ser

580 585 590

Pro Arg Asn Pro Thr Val Arg Arg Ser Leu Arg Phe Asp Ala Lys Asn

595 600 605

Asp Phe Ser Thr Arg Lys Lys His Arg Leu Gln Val Phe Gln Asp Ile

610 615 620

Thr Pro Ser Ile Asp Ser Pro

625 630

<210> 7

<211> 673

<212> PRT

<213> 野草莓（Fragaria vesca）

<400> 7

Met Trp Ala Arg Asp Arg Ser Tyr Pro Pro Ala Gly Phe Phe Thr Pro

1 5 10 15

Pro Pro Pro Arg Lys Ser Gly Pro Pro Asn Pro Pro Pro Met Ser Glu

20 25 30

Arg Lys Arg Val Ser Pro Ala Ala Ala Arg Ser Asp Leu Phe His Val

35 40 45

Ala His Lys Val Pro Ala Gly Asp Ser Pro Tyr Val Arg Ala Lys Gln

50 55 60

Val Gln Leu Ile Asp Lys Asn Pro Ser Lys Ala Ile Ser Leu Phe Trp

65 70 75 80

Ala Ala Ile Asn Ala Gly Asp Arg Val Asp Ser Ala Leu Lys Asp Met

85 90 95

Ala Val Val Met Lys Gln Leu Asp Arg Ala Glu Glu Ala Ile Glu Ala

100 105 110

Ile Lys Ser Phe Arg His Leu Cys Pro Tyr Glu Ser Gln Glu Ser Leu

115 120 125

Asn Asn Val Leu Val Glu Leu Tyr Lys Arg Ala Gly Arg Ile Glu Asp

130 135 140

Glu Ile Glu Thr Leu Gln Ser Lys Leu Lys Arg Met Asp Glu Gly Ile

145 150 155 160

Ala Phe Asn Gly Lys Arg Thr Lys Thr Ala Arg Ala Gln Gly Lys Lys

165 170 175

Val Gln Ile Thr Val Glu Gln Glu Arg Ser Arg Val Leu Gly Asn Leu

180 185 190

Ala Trp Ala Tyr Met Gln Gln Gly Asn Tyr Thr Thr Ala Glu Glu His

195 200 205

Tyr Lys Asn Ala Leu Ala Leu Glu Pro Asp Lys Asn Lys Gln Cys Asn

210 215 220

Leu Ala Ile Cys Leu Met His Met Asn Arg Ile Thr Glu Ala Arg His

225 230 235 240

Leu Leu Gln Ala Val Arg Asp Ser Ala Gly Asn Lys Pro Met Asp Glu

245 250 255

Ser Tyr Ala Lys Ser Phe Glu Arg Ser Phe Glu Met Leu Thr Glu Leu

260 265 270

Glu Gln Gln Ser Val Leu Arg Pro Ile Gln Gln Asn Glu Asn Tyr Cys

275 280 285

Thr Gly Ile Ser Arg Thr Pro Lys Pro Ser Gly Ser Gly Tyr Met Pro

290 295 300

Met Pro Pro Arg Arg Trp Thr Asp Gly Pro Glu Tyr Val Thr Val Ala

305 310 315 320

Asn Glu Arg His Arg Glu Ser Phe Arg Pro Gly Ser Cys Ala Lys Ser

325 330 335

Phe Asp Lys Trp Lys Lys Asp Tyr Asp Leu Lys Asn Thr Gly Asp Ser

340 345 350

Arg Pro Ser Phe Ser Ser Ser Val Lys Gln Asn Gln Asp Gly Met Thr

355 360 365

Val Thr Glu Val Asn Pro His Glu Lys Thr Tyr Val Ser Pro Val Leu

370 375 380

Tyr Thr Gln Pro Arg Arg Pro Ser Trp Gly Phe Asn Asp Gly His Gln

385 390 395 400

Arg Ser Glu Ile Trp Gly Asn Gly Val Gly Ser Ser Asn Lys Lys Leu

405 410 415

Pro Pro Glu Arg Ser Ala Gly Asn Val Arg Ala His Val Val Arg Asn

420 425 430

Leu Asn Ala Asp Leu Leu Ala Ser Thr Pro Arg Glu Ser Asp Ser Ser

435 440 445

Leu Ser Ser Arg Ser His Gly Asp Trp Arg Met Pro Gln Arg Asp Ala

450 455 460

Val Ala Arg Pro Val Leu Gln Pro Val Ser Ser Met Thr Ser Arg Gly

465 470 475 480

Asn Glu Gly Tyr Leu Pro Val Arg Asn Glu Ala Met Ile Asp Arg Ser

485 490 495

Ser Lys Tyr Thr Gly Asn Gly Asp Ile Arg Arg Val Thr Trp Asp Asn

500 505 510

Ala Gly Met Gln Lys Ser Ala Ala Gly Ser Gln Val Val Asp Glu Asn

515 520 525

Ala Lys Ala Leu Leu Leu Cys Ile Asp Glu Gly Asp Ser Gln Ser Ser

530 535 540

Gly Thr Thr Val Leu Gly Ser Met Glu Asn Met Asn Ala Ser Val Glu

545 550 555 560

Glu Asp Cys Leu Gly Asp Asn Ser Ser Gly Thr Leu Asp Asn Val His

565 570 575

Gln Ser Pro Ala Glu Asn Glu Arg Pro Thr Leu His Gln Ser Pro Ala

580 585 590

Glu Thr Glu Lys Pro Thr Pro Asp Phe Trp Lys Tyr Ser Gly Lys Lys

595 600 605

Ser Trp Ala Asp Met Val Glu Glu Glu Glu Glu Glu Leu Arg Ser Gly

610 615 620

Thr Thr Gly Tyr Phe Asp Ser Trp Asn Thr Gly Asp Glu Phe Asp Asp

625 630 635 640

Glu Asn Arg Asn Pro Asn Ile Met Thr Pro Gln Ser Pro Gln Phe Gln

645 650 655

Thr Gln Met Lys Arg Arg Asn Arg Leu Gln Ser Phe Gln Asp Ile Thr

660 665 670

Asn

<210> 8

<211> 601

<212> PRT

<213> 大豆（Glycine max）

<400> 8

Met Leu Phe Glu Arg Gly Ser Pro Ala Arg Cys Tyr Met Thr Pro Pro

1 5 10 15

Pro Gln Arg Thr Ser Pro Trp Lys Pro Pro His Ser Arg Ser Ser Ser

20 25 30

Val Pro Phe Ser Glu Arg Lys Met Ser Pro Asn Ser Val Asn Lys Ser

35 40 45

Asp Ile Phe His Ile Ile His Lys Val Pro Ala Gly Asp Ser Pro Tyr

50 55 60

Val Lys Ala Lys Gln Val Gln Leu Val Asp Lys Asp Pro Gly Arg Ala

65 70 75 80

Val Ser Leu Phe Trp Ala Ala Ile Asn Ala Gly Asp Arg Val Glu Ser

85 90 95

Ala Leu Lys Asp Met Ala Leu Val Met Lys Gln Leu Asn Arg Ser Asp

100 105 110

Glu Ala Ile Glu Ala Ile Arg Ser Phe Arg His Leu Cys Pro Ser Asp

115 120 125

Ser Gln Asp Ser Leu Asp Asn Ile Leu Val Glu Leu Tyr Lys Arg Ser

130 135 140

Gly Arg Val Asp Glu Glu Ile Ala Met Leu Cys His Lys Leu Lys Gln

145 150 155 160

Ile Glu Asp Gly Leu Thr Phe Val Gly Arg Thr Thr Lys Gln Ala Arg

165 170 175

Ser Gln Gly Lys Lys Ile Gln Ile Thr Ala Glu Gln Glu Ile Ser Arg

180 185 190

Ile Leu Gly Asn Leu Ala Trp Ala Tyr Leu Gln Lys Gly Asp Tyr Lys

195 200 205

Thr Ala Glu Glu His Tyr Arg Lys Ala Leu Ser Phe Glu Val Asp Arg

210 215 220

Asn Lys Gln Cys Asn Leu Ala Ile Cys Leu Ile His Met Asn Lys Ile

225 230 235 240

Lys Glu Ala Lys Phe Leu Leu Gln Ala Val Arg Thr Ala Thr Lys Asn

245 250 255

Arg Lys Met Asp Asp Ser Phe Ala Lys Ser Phe Glu Arg Ala Ser Gln

260 265 270

Met Leu Ile Glu Ile Glu Thr Ser Ser Ser Gln Asn Ala Ala Phe Ser

275 280 285

Met Thr Thr Gln Cys Pro Pro Gln Ser Phe Glu Asn Ser Ile Arg Met

290 295 300

Ser Ser Asp Ser Val Gln Ser Arg Ser Glu Asn Arg Ser Glu Ile Ser

305 310 315 320

Glu Gly Asp Ala Pro His Ala Arg Arg Arg Leu Tyr Gln Ser Pro Asp

325 330 335

Pro Ser Arg Arg Asp Leu Ser Val Pro Cys Thr Lys Pro Lys Arg Cys

340 345 350

Ser Trp Gly Phe Asn Asn Gly His Arg Arg Glu Ala Trp Gly Asp Ala

355 360 365

Asn Ser Asp Tyr Lys Pro Thr Phe Gly Thr Pro Pro Asn Asp Lys His

370 375 380

Val Thr Arg Met Leu Asn Ser Arg Glu Asn Gly Phe Ser Ser Pro Ala

385 390 395 400

Asn Gly Asn Gly Lys Ala Ala Thr Leu Glu Asp Pro Ala Ile Leu Lys

405 410 415

His Glu Ala Thr Lys Val Thr Ser Ser Asp Ser Leu His Thr Leu Asn

420 425 430

Thr Glu Ser Ala Thr Glu Phe Thr Glu Lys Glu Lys Ser Ala Ala Asp

435 440 445

Asp Ser Ser Tyr Gly Ser Ile Leu Ser Glu Ser His Val Thr Val Val

450 455 460

Asn Gly Val Asn Glu Phe Ala Ser Gly Lys Arg Lys Pro Glu Lys Lys

465 470 475 480

Ser Trp Ala Asp Ile Val Glu Glu Glu Gln Asn Glu Glu Asn Asp Phe

485 490 495

Phe Ser Gly Phe Thr Asn Phe Asp Asp Lys Asn Gly Ala Glu Val Phe

500 505 510

Asn Asp Glu Asn Glu Asp Ser Asn Ile Ile Tyr Pro Ser Pro Trp Pro

515 520 525

Gln Asn Gln Pro Asp Glu Trp Ser Ser Lys Lys Leu Glu Ser Leu Asp

530 535 540

Lys Lys Asp Gly His Tyr Ala Ser Glu Ser Ala Ile Leu Ser Arg Asn

545 550 555 560

Pro Thr Ala Arg Arg Ser Leu Cys Phe Asn Pro Glu Leu Ile Gly Glu

565 570 575

Lys Arg Leu Thr Arg Arg Ser Arg Leu Gln Val Phe Gln Asp Ile Thr

580 585 590

Leu Leu Pro Glu Thr Pro Arg Phe Ala

595 600

<210> 9

<211> 602

<212> PRT

<213> 大豆（Glycine max）

<400> 9

Met Gln Phe Glu Arg Gly Ser Met Ala Arg Cys Tyr Met Thr Pro Pro

1 5 10 15

Pro Gln Pro Pro Ser Ser Trp Lys Pro Pro His Ser Gln Ser Pro Ser

20 25 30

Val Pro Phe Ser Glu Arg Lys Lys Ser Pro Asn Ser Ala Asn Lys Ile

35 40 45

Asp Leu Phe His Ile Ile His Lys Val Pro Ala Gly Asp Ser Pro Tyr

50 55 60

Val Lys Ala Lys Gln Val Gln Leu Val Asp Lys Asp Pro Gly Arg Ala

65 70 75 80

Ile Ser Leu Phe Trp Ala Ala Ile Asn Ala Arg Asp Arg Val Glu Ser

85 90 95

Ala Leu Lys Asp Met Ala Leu Val Met Lys Gln Leu Asn Arg Ser Asp

100 105 110

Glu Ala Ile Glu Ala Ile Arg Ser Phe Arg His Leu Cys Pro Ser Asp

115 120 125

Ser Arg Asp Ser Leu Asp Asn Ile Leu Val Glu Leu Tyr Lys Arg Ser

130 135 140

Gly Arg Ile Asp Glu Glu Ile Ala Met Leu His His Lys Leu Lys Gln

145 150 155 160

Ile Glu Asp Gly Leu Thr Phe Val Gly Arg Thr Thr Lys Gln Ala Arg

165 170 175

Ser Gln Gly Lys Lys Ile Gln Ile Thr Ala Glu Gln Glu Ile Ser Arg

180 185 190

Ile Leu Gly Asn Leu Ala Trp Ala Tyr Leu Gln Lys Gly Asp Tyr Lys

195 200 205

Ala Ala Glu Glu His Tyr Arg Lys Ala Leu Ser Phe Glu Val Asp Arg

210 215 220

Asn Lys Gln Cys Asn Leu Ala Ile Cys Leu Ile His Met Asn Lys Ile

225 230 235 240

Lys Glu Ala Lys Phe Leu Leu Gln Ala Val Arg Thr Ala Thr Lys Asn

245 250 255

Arg Lys Met Asp Asp Ser Phe Ala Lys Ser Phe Glu Arg Ala Ser Gln

260 265 270

Met Leu Ile Glu Ile Glu Thr Ser Ser Ser Ser Gln Asn Ala Ala Phe

275 280 285

Ser Met Thr Thr Met Ser Gln Cys Ser Pro Gln Ser Phe Glu Asn Ser

290 295 300

Ile Arg Lys Ser Ser Asp Arg Val Gln Ser Gly Ser Glu Ser Arg Ser

305 310 315 320

Glu Thr Ser Glu Gly Asp Val Pro His Ala Arg Arg Arg Leu Tyr Gln

325 330 335

Ser Pro Asp Pro Ser Arg Arg Asp Leu Ser Val Pro Cys Thr Lys Pro

340 345 350

Lys Arg Cys Ser Trp Gly Phe Asn Thr Gly Tyr Arg Arg Glu Ala Trp

355 360 365

Gly Asp Val Asn Ser Asp Tyr Lys Pro Ser Phe Gly Thr Pro Pro Asn

370 375 380

Asp Lys His Ala Thr Arg Met Leu Asn Ser Arg Glu Asn Gly Leu Ser

385 390 395 400

Ser Pro Ala Asn Gly Lys Trp Arg Ala Met Thr Leu Glu Asp Gln Ala

405 410 415

Ile Leu Lys His Glu Ala Thr Thr Ala Thr Ser Ser Asp Ser Leu His

420 425 430

Ala Leu Asn Thr Glu Val Ala Met Glu Phe Thr Glu Lys Glu Lys Ser

435 440 445

Ala Ala Asp Asp Ser Ser Tyr Arg Ser Ile Leu Ser Glu Ser His Val

450 455 460

Thr Val Val Asn Gly Ala Asn Glu Phe Ala Ser Gly Lys Arg Lys Pro

465 470 475 480

Glu Lys Lys Ser Trp Ala Asp Ile Val Glu Glu Glu Gln Asn Glu Glu

485 490 495

Tyr Asp Phe Leu Ser Gly Phe Thr Asn Phe Asp Asp Lys Asn Gly Ala

500 505 510

Glu Val Phe Asn Asp Glu Asn Glu Asp Ser Asn Ile Ile Tyr Pro Ser

515 520 525

Ser Trp Leu Gln Asn Leu Pro Glu Trp Ser Asn Lys Lys Leu Glu Ser

530 535 540

Ser Asp Lys Lys Asp Asp Tyr Ala Ser Glu Ser Thr Ile Leu Ser Arg

545 550 555 560

Asn Pro Thr Ala Arg Arg Ser Leu Cys Phe Asn Pro Glu Leu Ile Gly

565 570 575

Glu Lys Lys Leu Thr Arg Arg Ser Arg Leu Gln Val Phe Gln Asp Ile

580 585 590

Thr Leu Leu Pro Glu Thr Pro Arg Phe Ala

595 600

<210> 10

<211> 603

<212> PRT

<213> 莲藕（Lotus japonicus）

<400> 10

Met Pro Phe Glu Arg Asn Ser Pro Ala Arg Cys Phe Met Thr Pro Pro

1 5 10 15

Pro Pro Arg Leu Ser Ser Trp Arg Ser Thr Pro Ser Arg Ser Pro Ile

20 25 30

Met Met Pro Leu Ser Glu Arg Lys Arg Ser Ser Pro Asn Lys Asp Asp

35 40 45

Pro Tyr His Val Ile His Lys Val Pro Ala Gly Asp Ser Pro Tyr Val

50 55 60

Lys Ala Lys Gln Val Gln Leu Val Asp Lys Asp Pro Gly Lys Ala Ile

65 70 75 80

Ser Leu Phe Trp Ala Ala Ile Asn Ala Gly Asp Arg Val Glu Ser Ala

85 90 95

Leu Lys Asp Met Ala Leu Val Met Lys Gln Leu Asn Arg Ser Asp Glu

100 105 110

Ala Ile Glu Ala Ile Lys Ser Phe Arg His Leu Cys Pro Pro Asp Ser

115 120 125

Gln Glu Ser Leu Asp Asn Ile Leu Val Glu Leu Tyr Lys Arg Ser Gly

130 135 140

Arg Val Asp Glu Glu Ile Ser Met Leu His His Lys Leu Lys Gln Ile

145 150 155 160

Glu Asp Gly Val Thr Phe Val Gly Arg Thr Thr Lys Gln Ala Arg Ser

165 170 175

Gln Gly Lys Lys Ile His Val Thr Ala Glu Gln Glu Ile Ser Arg Ile

180 185 190

Leu Gly Asn Leu Ala Trp Ala Tyr Leu Gln Lys Gly Asp Tyr Lys Ala

195 200 205

Ala Glu Glu His Tyr Arg Lys Ala Leu Ser Phe Glu Val Asp Arg Asn

210 215 220

Lys Gln Cys Asn Leu Ala Ile Cys Leu Met Gln Thr Asn Arg Ile Thr

225 230 235 240

Glu Ala Lys Phe Leu Leu Gln Ala Val Thr Thr Ala Ser Lys His Arg

245 250 255

Lys Met Asp Asp Ser Cys Ala Lys Ser Phe Glu Arg Ala Ser Gln Met

260 265 270

Leu Met Asp Met Glu Ser Ser Ser Ser Gln Cys Ser Pro Leu Ser Asn

275 280 285

Leu Ser Gly Lys Ser Ser Asp Met Val Gln Ser Arg Thr Gln Asn Trp

290 295 300

Ser Val Thr Ser Glu Gly Glu Val Ser Asn Ala Arg Arg Arg Leu Tyr

305 310 315 320

Glu Ser Pro Glu Pro Ala Arg Arg Asp Leu Lys Val Pro Tyr Thr Asn

325 330 335

Thr Lys Lys Cys Ser Trp Gly Phe Asn Asn Gly Pro Gln Arg Glu Thr

340 345 350

Trp Gly Asp Val His Ser Asp Pro Lys Pro Ser Phe Gly Ile Pro Ser

355 360 365

Lys Gln Asn Asn Leu Ser Leu Pro Ala Asn Gly Lys Trp Asn Ala Arg

370 375 380

Thr Met Val Lys Arg Glu Asp Lys Thr Ala Ile Gly Ser Gly Leu Tyr

385 390 395 400

Thr Tyr Ser Asn Thr Glu Thr Asp Arg Lys Phe Lys Glu Asp Lys Phe

405 410 415

Ala Val Glu Ser Asn Asp Met Val Val Asn Gly Ala Asn Gln Phe Ala

420 425 430

Ser Ser Ile Gly Thr Asp Gln Ser Lys Cys Ser Glu Ile Ser Ile Lys

435 440 445

Leu Cys Cys Tyr Pro Phe Cys Gln Leu Asn Ser Ser Leu Ile Asn Leu

450 455 460

His Glu Leu Ala Ser Gly Ser Ile Lys Pro Val Glu Lys Lys Ser Trp

465 470 475 480

Ala Asp Ile Val Glu Glu Glu Gln Asn Glu Gly His Asp Phe Phe Gly

485 490 495

Gly Tyr Thr Arg Phe Asp Gly Gln Glu Gly Ala Gln Val Phe Asn Lys

500 505 510

Glu Asn Glu Asn Ser Asn Ile Val Phe Gln Arg Pro Trp Pro Leu Ser

515 520 525

Glu Thr Lys Cys Ile Ser Lys Lys Leu Glu Ser Met Asp Leu Lys Asp

530 535 540

Gly His His Gly Ser Gly Thr Val Thr Leu Ser Arg Lys Pro Ala Val

545 550 555 560

Arg Arg Ser Leu Cys Phe Asn Pro Glu Leu Ala Lys Glu Arg Asp Ser

565 570 575

Ser Leu Ser Glu Glu Lys Lys Pro Pro Arg Asn Asn Arg Leu Gln Val

580 585 590

Phe Lys Asp Ile Ala Leu His Pro Glu Thr Pro

595 600

<210> 11

<211> 617

<212> PRT

<213> 蒺藜苜蓿（Medicago truncatula）

<400> 11

Met Thr Phe Glu Arg Asn Ser Pro Ala Arg Cys Tyr Met Thr Pro Pro

1 5 10 15

Ser Ser Ser Ser Ser Trp Lys Ser Arg Pro Val Arg Ser Pro Thr Val

20 25 30

Pro Phe Ser Glu Arg Lys Lys Ser Pro Ala Ala Ser Val Ser Lys Asp

35 40 45

Asp Leu Phe His Val Ile His Lys Val Pro Ser Gly Asp Ser Pro Tyr

50 55 60

Val Lys Ala Lys Gln Val Gln Leu Val Asp Lys Asp Pro Gly Lys Ala

65 70 75 80

Ile Ser Leu Phe Trp Ala Ala Ile Asn Ala Gly Asp Arg Val Glu Ser

85 90 95

Ala Leu Lys Asp Met Ala Leu Val Met Lys Gln Leu Asn Arg Ser Asp

100 105 110

Glu Ala Ile Glu Ala Ile Lys Ser Phe Arg His Leu Cys Pro Ser Asp

115 120 125

Ser Gln Glu Ser Leu Asp Asn Ile Leu Val Glu Leu Tyr Lys Arg Ser

130 135 140

Gly Arg Val Asp Glu Glu Ile Gly Met Leu His Gln Lys Leu Lys Gln

145 150 155 160

Ile Glu Asp Gly Met Thr Tyr Val Gly Arg Thr Thr Lys His Ala Arg

165 170 175

Ser Gln Gly Lys Lys Ile Gln Ile Ser Ala Glu Gln Glu Ile Ser Arg

180 185 190

Ile Leu Gly Asn Leu Ala Trp Ala Tyr Leu Gln Lys Gly Asp Tyr Lys

195 200 205

Thr Ala Glu Glu His Tyr Arg Lys Ala Leu Ser Phe Glu Val Asp Arg

210 215 220

Asn Lys Gln Cys Asn Leu Ala Ile Cys Leu Met Gln Met Asn Lys Val

225 230 235 240

Thr Glu Ala Arg Phe Leu Leu Gln Ala Val Thr Ala Ala Thr Lys Asn

245 250 255

Arg Lys Met Asp Asp Ser Phe Val Lys Ser Tyr Glu Arg Ala Thr Gln

260 265 270

Met Leu Gln Glu Met Glu Ser Thr Ala Pro Ser Val Asp Ser Val Lys

275 280 285

Asp Lys Gly Asp Asn Lys Phe Asn Glu Thr Glu Arg Phe Ser Gly Arg

290 295 300

Asn Met Ser Ser Pro Tyr Ser Thr Pro Asn Leu Glu Ser Ser Asn Gly

305 310 315 320

Lys Thr Thr Gly Thr Val Lys Ser Arg Thr Glu Asn Asn Arg Ser Leu

325 330 335

Thr Ser Asp Ala Lys Asp Ser His His Ser His Ala Arg Arg Arg Leu

340 345 350

Tyr Glu Ser Leu Asp Pro Ala Lys Ser Asp Pro Lys Val Pro Pro Tyr

355 360 365

Thr Lys Pro Lys Arg Pro Ser Trp Gly Phe Asn Ser His Ser Asp Ser

370 375 380

Lys Pro Ser Phe Val Ser Tyr Pro Asn Glu Lys Ala Pro Tyr Ile Ile

385 390 395 400

Lys Pro Asn Ser Thr Gln Asn Gly Phe Ser Pro Arg Thr Ala Thr Asn

405 410 415

Trp Arg Thr Arg Thr Pro Glu Gly Asp Ala Ala Ile Val Lys Tyr Gly

420 425 430

Pro Thr Thr Thr Val Lys Gln Gly Asn Lys Thr Thr Val Phe Ser Ser

435 440 445

Gly Ser Ile Tyr Pro Leu Asn Thr Glu Ala Ala Met Lys Phe Thr Lys

450 455 460

Asn Asp Asn Asn Asn Lys Phe Thr Val Thr Asn Glu Phe Ala Ala Ser

465 470 475 480

Val Asp Thr Lys Asp Gln Asn Gln Asp Lys Lys Leu Ala Lys Lys Ser

485 490 495

Trp Ala Asp Met Val Glu Glu Glu Gln Gln Ser Glu Glu Tyr Glu Leu

500 505 510

Phe Tyr Lys Gly Tyr Thr Asn Phe Asp Ala Gln Val Phe Gln Asn Glu

515 520 525

Asn Glu Asn Ser Asn Ile Val Tyr Gln Pro Pro Ser Arg Arg Ser His

530 535 540

Tyr Glu Thr Glu Ser Leu Asn Gln Asn Leu Glu Phe Met Asn Leu Lys

545 550 555 560

Asp Gly Tyr Asn Ala Ala Pro Val Asn Asp Thr Trp Leu Arg Asn Pro

565 570 575

Thr Val Arg Arg Ser Leu Phe Thr Asn Ala Glu Met Thr Asn Glu Arg

580 585 590

Asp Val Phe Ser Gly Glu Glu Lys Arg Thr Arg Arg Ala Arg Leu Gln

595 600 605

Val Phe Gln Asp Ile Thr Ser Ser Ser

610 615

<210> 12

<211> 673

<212> PRT

<213> 葡萄（Vitis vinifera）

<400> 12

Met Trp Ser His Asn Asn Asn Phe Pro Ala Lys Gly Phe Ser Thr Pro

1 5 10 15

Pro Pro Thr Trp Lys Ser Lys Ala Ser Phe Ser Ser Ala Thr Pro Val

20 25 30

Ser Glu Lys Thr Arg Ser Met Ala Asn Lys Asp Asp Leu Phe His Val

35 40 45

Val His Lys Val Pro Ala Gly Asp Ser Pro Tyr Val Arg Ala Lys Gln

50 55 60

Val Gln Leu Ile Asp Lys Asp Pro Asn Arg Ala Ile Ser Leu Phe Trp

65 70 75 80

Ala Ala Ile Asn Ser Gly Asp Arg Val Asp Ser Ala Leu Lys Asp Met

85 90 95

Ala Val Val Met Lys Gln Leu Asn Arg Ser Asp Glu Ala Ile Glu Ala

100 105 110

Ile Lys Ser Phe Arg His Leu Cys Pro Gln Glu Ser Gln Glu Ser Leu

115 120 125

Asp Asn Val Leu Val Glu Leu Tyr Lys Arg Ser Gly Arg Leu Asp Glu

130 135 140

Gln Ile Glu Met Leu Gln Tyr Lys Leu Lys Asn Ile Asp Glu Gly Ser

145 150 155 160

Ala Phe Gly Gly Lys Arg Thr Lys Ile Ala Arg Ser Gln Gly Lys Lys

165 170 175

Ile Gln Ile Ser Ile Glu Gln Glu Lys Ser Arg Leu Leu Gly Asn Leu

180 185 190

Ala Trp Ala Tyr Leu Gln Gln Gly Asn Tyr Lys Thr Ala Gly Glu Leu

195 200 205

Tyr Lys Gln Ala Leu Ala Leu Asp Pro Asp Arg Asn Lys Glu Cys Asn

210 215 220

Leu Ala Ile Cys Leu Met Tyr Met Asn Lys Ile Lys Glu Ala Lys Ala

225 230 235 240

Met Leu Tyr Ala Ile Gln Val Ser Ser Gln Asn Gly Arg Met Asp Asp

245 250 255

Ser Tyr Val Lys Ser Phe Glu Arg Ala Ser Gln Val Leu Thr Glu Leu

260 265 270

Glu Ala Asn Ser Val Ile Asp Pro Asn Glu Gln Glu Gly His Glu Glu

275 280 285

Met Arg Arg His Leu Arg Ser Leu Val Ser Arg Asn Ser Ile Glu Val

290 295 300

Asn Ser Cys Ile Asn Glu Glu Asn Asp His Leu Ser Gly Leu Val Ala

305 310 315 320

Ser Arg Arg Arg Ala Gly Arg Gln Gln Glu Glu Thr Met Leu Leu Asp

325 330 335

Lys Pro Asn Arg Arg Ser Tyr Cys Gln Asn Gln Phe Glu Asn Lys Asp

340 345 350

Asn Phe Ser Gln Pro Asp Glu Glu Ser Ser Lys Cys Met Ser Leu Gly

355 360 365

Leu Ser Ser Ala Gln Ser Pro Gln Asn Leu Tyr Ala Asp Lys Trp Lys

370 375 380

Lys Gly Ala Gln Leu Glu Asn Pro Phe Glu Arg Ser Asp Phe Ser Ser

385 390 395 400

Arg Arg Lys Gly Asn Trp Val Ser Ala Thr Asp Lys Val Gly Ser Val

405 410 415

Gln Arg Arg Thr Tyr Gly Ser Pro Leu Pro Val Arg Gly Asn Ser Lys

420 425 430

Leu Pro Ser Thr Glu Gln Arg Arg Gly Pro Cys Leu Leu Ser Lys Ala

435 440 445

Asp Gln Arg Lys Ser Thr Trp Gly Glu Asn Thr Ala Asp Ser Pro Gly

450 455 460

Arg Lys Leu Ser Phe Glu Asp Pro Ile Ala Lys Glu Ala Gly Ala Met

465 470 475 480

Ala Pro Gln Asn Pro Asp Gly Arg Leu Gln Ala Ser Ser Asn Glu Lys

485 490 495

Leu Lys Ile Ala Leu Gln Thr Ser Glu Lys Ser Leu Pro Ser Pro Gly

500 505 510

Gly Phe Asp Gly Lys Cys Phe Arg Glu Asn Ser Gly Lys Leu Met Ser

515 520 525

Leu Gln Gln Val Glu Gly Asn Pro Gln Leu Pro Asn Gln Asp Ser Ser

530 535 540

Thr Ser Lys Asn Lys Met Ser Trp Ala Asp Met Leu Asp Tyr Cys Tyr

545 550 555 560

Gln Lys Pro Ser Phe Ser Phe Gln Thr Pro Asn Lys Trp Tyr Asp Gly

565 570 575

Trp Ser His Gly Glu Asp Phe Asn Asp Glu Asn Leu Asn Ser Asn Ile

580 585 590

Phe His Gln Thr Pro Pro Ser Val His Glu Ile Asp Asn Val Ser Tyr

595 600 605

Lys Leu Glu Ala Phe Asp Leu Lys Asp Gly Tyr Asn Thr Pro Gly Ser

610 615 620

Asp Val Ser Ser Arg Asn Asn Pro Thr Ala Arg Arg Ser Leu Ser Asn

625 630 635 640

Glu Thr Met Ser Thr Ser Gly Ser Thr Ile Arg Pro Lys Arg Arg Asn

645 650 655

Arg Leu Gln Val Phe Arg Asp Ile Thr Leu His Thr Glu Ser Pro Arg

660 665 670

Thr

<210> 13

<211> 773

<212> PRT

<213> 黄瓜（Cucumis sativus）

<400> 13

Met Leu Thr Asn Ser Gly Lys Asn Lys Phe Leu Cys Lys Gly Phe Ser

1 5 10 15

Thr Pro Pro Pro Ser Trp Lys Trp Lys Pro Phe Arg Leu Pro Lys Thr

20 25 30

Ala Pro Phe Ser Glu Ser Lys Arg Leu Ser Pro Asn Phe Ala Asn Lys

35 40 45

Ser Asp Leu Phe His Val Ile His Lys Val Pro Ala Gly Asp Ser Pro

50 55 60

Tyr Val Lys Ala Lys Gln Val Gln Leu Ile Asp Lys Asp Pro Asn Arg

65 70 75 80

Ala Val Ser Leu Phe Trp Ala Ala Ile Asn Ala Gly Asp Arg Val Asp

85 90 95

Ser Ala Leu Lys Asp Met Ala Val Val Met Lys Gln Leu Asp Arg Ser

100 105 110

Asp Glu Ala Ile Glu Ala Ile Lys Ser Phe Arg His Leu Cys Pro Tyr

115 120 125

Asp Ser Gln Glu Ser Ile Asp Asn Val Leu Ile Glu Leu Tyr Lys Arg

130 135 140

Ser Gly Arg Ile Glu Glu Glu Ile Asp Met Leu Gln Cys Lys Leu Lys

145 150 155 160

Gln Ile Glu Asp Gly Thr Val Phe Gly Gly Lys Arg Thr Lys Ala Ala

165 170 175

Arg Ser Gln Gly Lys Lys Val Gln Ile Thr Val Glu Gln Glu Lys Ser

180 185 190

Arg Val Leu Gly Asn Leu Ala Trp Ala Phe Leu Gln Leu Asp Asn Ile

195 200 205

Tyr Ile Ala Glu Glu Tyr Tyr Arg Lys Ala Leu Ser Leu Glu Ser Asp

210 215 220

Asn Asn Lys Lys Cys Asn Leu Ala Ile Cys Leu Ile Leu Thr Asn Arg

225 230 235 240

Leu Thr Glu Ala Lys Ser Leu Leu Gln Ser Val Arg Ala Ser Ser Gly

245 250 255

Gly Lys Pro Met Glu Glu Ser Tyr Ala Lys Ser Phe Glu Arg Ala Ser

260 265 270

His Met Leu Ala Glu Lys Glu Ser Lys Ser Phe Asn Ser Thr Glu His

275 280 285

Glu Glu Asp Asn Asn Thr Ala Ala Thr Ile Thr Ser Lys Asn Thr Thr

290 295 300

Gly Lys Ser Gly Arg Cys Val Pro Gln Ile Thr Ala Ser Thr Lys Trp

305 310 315 320

Thr Arg Asp Asp Glu Arg Met Tyr Ile Asn Glu Asn Ser Trp Asp Asp

325 330 335

Asp His His Trp Asp Cys Tyr Glu Asn Lys Ser Ile Gly Ala Val Asn

340 345 350

Ser Ser His Asn Tyr Leu His Cys Asp Lys Trp Ser Glu Gly Cys Phe

355 360 365

Ile Glu Asn Leu Gly Lys Thr Asp Ser Cys Ile Pro Ile Lys Ile Lys

370 375 380

Gly Asp Arg Asn Gln Gly Gly Leu Phe Arg Leu Glu Asp Glu Ser Phe

385 390 395 400

Asn Cys Cys Ser Leu Phe Ser Ser Pro Thr Pro Ala Lys Arg Ser Val

405 410 415

Glu Val Pro Phe Thr Gln Pro Lys Asn Ser Phe Trp Glu Phe Asn Asn

420 425 430

Arg Trp Gly Ser Lys Glu Arg Lys Gln Gln Arg Lys Arg Ile Arg Lys

435 440 445

Val Leu Phe Gly Asn Pro Ser Lys Lys Asn Lys Ser Phe Asp Ser Gly

450 455 460

Phe Leu Val Asp Ser Ser Ser Glu Ser Glu Gly Thr Lys Pro Thr Ser

465 470 475 480

Asn Tyr Lys Thr Lys Tyr Arg Ser Ala Ala Pro Asp Ser Val Glu Leu

485 490 495

Glu Val Pro Phe Thr Gln Pro Arg Ser Cys Glu Trp Val Met Asn Arg

500 505 510

His Ser Arg Lys Ala Thr Glu Cys Phe Arg Ser Leu Arg Ser Ser Ser

515 520 525

Ser Ser Arg Lys Leu Ser Phe Glu Pro Pro Thr Ser Thr Glu Asn Ile

530 535 540

Gln Thr Thr Asn Asp Ser Asn Phe Gly Arg Phe Glu Leu Ser Arg Ala

545 550 555 560

Val Ser Asp Glu Pro Gln Asp Leu Glu Gly Asp Trp Asn Gln Thr Ser

565 570 575

Cys Gly Asp Ile Lys Tyr Glu Glu Gly Gly Ser Pro Met Leu Tyr Gly

580 585 590

Leu Met Lys Lys Ile Lys Glu Glu Cys Ile Ala Val Asp Gln Lys Leu

595 600 605

Gln His Asn Ser Pro Thr Val Phe Gly Lys Lys Ser Trp Ala Asp Met

610 615 620

Val Glu Glu Glu Glu Glu Glu Lys Glu Met Ser Ser Ser Ser Gly Ser

625 630 635 640

Asp Gln Val Asn Cys Phe Ala Asp Asn Trp Ser Cys Ser Ser Asp Asp

645 650 655

Asn Gly Glu Phe Lys Phe Asn Asp Glu Asn Leu Asn Ser Asn Ile Leu

660 665 670

His Gln Asn His Cys Pro Pro Ser Ser Asn Gln Leu Glu Asp Thr Ile

675 680 685

Lys Ile Gly Ser Leu Glu Ile Lys Asp Asp Ser Asp Glu Val Val Ser

690 695 700

Ser Arg Asn Ser Val Glu Arg Cys Pro Leu Tyr Phe Asp Gln Gln Gln

705 710 715 720

Gln Pro Thr Leu Glu Ser Ile Asp Asn Cys Cys Ala Ser Pro Leu Pro

725 730 735

Arg Lys Asp Leu Thr Thr Glu Val Ser Cys Lys Phe Gly Gln Glu Asn

740 745 750

Lys Leu Met Arg Gly Asn Arg Leu Gln Val Phe His Glu Ile Thr Ser

755 760 765

Val His Gln Glu Ser

770

<210> 14

<211> 683

<212> PRT

<213> 黄瓜（Cucumis sativus）

<400> 14

Met Trp Thr Asn Asn Ser Lys Asn Asn Phe Pro Cys Lys Gly Phe Leu

1 5 10 15

Thr Pro Pro Pro Ser Trp Lys Ser Gly Pro Phe Arg Ser Pro Lys Thr

20 25 30

Ala Pro Phe Ser Glu Arg Lys Arg Ser Ser Pro Asn Phe Ala Asn Lys

35 40 45

Ser Asp Leu Phe His Val Ile His Lys Val Pro Ala Gly Asp Ser Pro

50 55 60

Tyr Val Lys Ala Lys Gln Val Gln Leu Ile Glu Lys Asp Pro Ser Arg

65 70 75 80

Ala Val Ser Leu Phe Trp Ala Ala Ile Asn Ala Gly Asp Arg Val Asp

85 90 95

Ser Ala Leu Lys Asp Met Ala Val Val Met Lys Gln Leu Asp Arg Ser

100 105 110

Asp Glu Ala Ile Glu Ala Ile Lys Ser Phe Arg His Leu Cys Pro Tyr

115 120 125

Asp Ser Gln Glu Ser Ile Asp Asn Val Leu Ile Glu Leu Tyr Lys Arg

130 135 140

Ser Gly Arg Ile Glu Glu Glu Ile Asp Met Leu Gln Arg Lys Leu Lys

145 150 155 160

Gln Ile Glu Asp Gly Thr Ile Phe Gly Gly Lys Arg Thr Lys Ala Ala

165 170 175

Arg Ser Gln Gly Lys Lys Val Gln Ile Thr Ile Glu Gln Glu Lys Ser

180 185 190

Arg Val Leu Gly Asn Leu Ala Trp Ala Phe Leu Gln Leu Asn Asn Ile

195 200 205

Tyr Val Ala Glu Asp Tyr Tyr Arg Lys Ala Leu Ser Leu Glu Ala Asp

210 215 220

Asn Asn Lys Lys Cys Asn Leu Ala Ile Cys Gln Ile Leu Thr Asn Arg

225 230 235 240

Leu Thr Glu Ala Lys Ser Leu Leu Gln Ser Val Arg Ala Ser Ser Gly

245 250 255

Gly Lys Pro Met Glu Glu Ser Tyr Ala Lys Ser Phe Glu Arg Ala Phe

260 265 270

His Met Leu Thr Glu Lys Glu Ser Lys Ser Phe Asn Ser Thr Gly Asn

275 280 285

Glu Glu Asp Asn Gly Ala Gly Thr Thr Ile Thr Ser Lys Asn Ala Thr

290 295 300

Gly Arg Thr Gly His Cys Val Pro Gln Ile Ala Ala Ser Thr Arg Trp

305 310 315 320

Thr Arg Asp Asp Glu Gln Met Tyr Ile Asn Glu Asn Ser Arg Asp Ile

325 330 335

Asp Pro His Trp Asp Cys Cys Asp Asp Lys Ser Val Gly Ala Val Asn

340 345 350

Ser Ser His Asn Tyr Leu His Ser Asp Lys Trp Ile Glu Gly Cys Cys

355 360 365

Ile Glu Asn Leu Gly Lys Thr Val Ser Cys Met Pro Ile Lys Met Lys

370 375 380

Gly Asn Arg Asn Arg Asp Ser Leu Phe Arg Leu Val Glu Glu Ser Phe

385 390 395 400

Asn Cys Cys Ser Leu His Thr Ser Pro Thr Pro Thr Lys Lys Asn Val

405 410 415

Glu Val Pro Phe Thr Gln Gln Lys Asn Ser Phe Trp Glu Phe Asn Thr

420 425 430

Arg Trp Arg Ser Lys Glu Arg Lys Gln Gln Gln Lys Arg Thr Arg Lys

435 440 445

Val Leu Phe Glu Asn Pro Ser Arg Lys Asp Gln Ser Phe Asp Ser Gly

450 455 460

Phe Val Val Asp Tyr Ser Ser Glu Ser Asp Glu Thr Glu Pro Ala Ser

465 470 475 480

Asn Tyr Lys Thr Lys Tyr Arg Ser Ala Ala Pro Asp Ser Ile Glu Leu

485 490 495

Glu Val Pro Phe Thr Gln Pro Arg Ser Cys Ser Trp Gly Met Asn Gly

500 505 510

Gly Gly Asn Ser Arg Lys Thr Thr Glu Cys Phe Arg Ser Leu Leu Ser

515 520 525

Arg Ser Ser Ser Arg Lys Leu Ser Phe Glu Leu Pro Thr Ser Thr Glu

530 535 540

Asn Thr Gln Ala Met Thr Asp Ser Asn Leu Gly Arg Ser Lys Leu Ser

545 550 555 560

Arg Glu Ile Ser Asp Glu Pro Gln Asp Leu Ala Gly Gly Asp Trp Lys

565 570 575

Gln Thr Ser Tyr Gly Asp Ile Glu Tyr Glu Glu Gly Thr Ile Pro Asn

580 585 590

Asp Ser Met Lys Ile Met Glu Glu His Met Thr Ile Asp His Lys Phe

595 600 605

Lys His Asn Thr Pro Thr Val Gly Gly Lys Lys Ser Trp Ala Asp Met

610 615 620

Val Glu Glu Glu Glu Glu Asp Ser Asp Asp Lys Asn Glu Asp Asp Thr

625 630 635 640

Glu Glu Thr Leu Ser Ser Ser Gly Arg Gly Gln Val Asn Cys Phe Asp

645 650 655

Asp Asn Trp Ser Ser Ser Ser Asp Asn Val Glu Tyr Lys Phe Asn Asp

660 665 670

Glu Thr Leu Thr Val His Gln Glu Leu Glu Cys

675 680

<210> 15

<211> 644

<212> PRT

<213> 桉树（Eucalyptus grandis）

<400> 15

Met Trp Gly Asn Arg Glu Asn Phe Pro Ala Arg Gly Tyr Phe Thr Pro

1 5 10 15

Gln Pro Pro Lys Arg Ala Val Arg Pro Leu Val Ser Pro Val Leu Met

20 25 30

Ser Glu Arg Lys Arg Ala Ser Pro Pro Asp Leu Phe His Ile Ile His

35 40 45

Lys Val Pro Ala Gly Asp Ser Pro Tyr Val Lys Ala Lys Arg Val Gln

50 55 60

Leu Ile Asp Lys Asp Pro Ser Lys Ala Ile Ser Leu Phe Trp Ala Ala

65 70 75 80

Ile Asn Ala Gly Asp Arg Val Asp Ser Ala Leu Lys Asp Met Ala Ile

85 90 95

Val Met Lys Gln Leu Asn Arg Ser Asp Glu Ala Ile Glu Ala Ile Lys

100 105 110

Ser Phe Arg His Leu Cys Pro Phe Asp Ser Gln Glu Ser Leu Asp Asn

115 120 125

Val Leu Ile Glu Leu Tyr Lys Arg Ser Gly Arg Ile Glu Glu Glu Ile

130 135 140

Glu Met Leu Gln His Lys Leu Arg Leu Ile Glu Glu Gly Lys Gly Phe

145 150 155 160

Val Ala Asn Arg Thr Lys Thr Ala Arg Ser Gln Gly Lys Lys Ile Gln

165 170 175

Val Thr Arg Glu Gln Glu Arg Ser Arg Ile Met Gly Asn Leu Ala Trp

180 185 190

Ala His Leu Gln Leu Ser Asn Tyr Glu Ile Ala Glu Gly Leu Tyr Arg

195 200 205

Glu Ala Leu Ser Leu Glu Pro Asp Lys Asn Lys Gln Cys Asn Leu Ala

210 215 220

Ile Cys Leu Met Asn Met Asn Lys Leu Ala Asp Ala Lys Ala Val Leu

225 230 235 240

Asp Ala Val Arg Gly Pro Cys Gly Asp Gly Asp Met Asp Glu Ser Tyr

245 250 255

Ala Lys Ser Phe Asp Arg Ala Leu Gln Met Leu Asn Glu Ile Lys Ser

260 265 270

Arg Pro Ile Lys Gln Asn Glu Val Asp His Lys Glu Ile Gln Asn Ser

275 280 285

Arg Glu Ser Thr Cys Phe Thr Glu Gln Val Ile Asn Arg Gly Ala Arg

290 295 300

Pro Leu Ala Ser Thr Thr Thr Ala Asn Gly His Gly Glu Glu Thr Trp

305 310 315 320

Ile Leu Asn Glu Arg Asn Gly Met Leu Ser Arg Ala Glu Ala Trp Ser

325 330 335

Ser Ser Glu Asn Ile Phe Gly Asp Asn Trp Arg Glu Val Ser Asn Phe

340 345 350

Gly Thr Pro Leu Arg Tyr Val Leu Pro Gly Asn Leu His Ala Lys Glu

355 360 365

Asn Cys Leu Glu Thr Ser Lys Val Gly Trp Ser Ser Ser Ser Lys Ser

370 375 380

Ala Tyr Val Ser Pro Ala Ser Ile Lys Arg Asn Ile Glu Phe Ser Pro

385 390 395 400

Gly Glu Ser Arg Arg His Thr Cys Arg Ser Leu Tyr Ala Ser Pro Ala

405 410 415

Ser Cys Arg Asp Ser Glu Tyr Lys Ser Lys Ala Thr Ser Thr Gly Glu

420 425 430

Thr Lys Leu Val Gly Asn Gly Ser Val Ala Met Ala Lys Pro Lys Asn

435 440 445

Tyr Asp Glu Asn Met Ile Arg Ser Lys Gly Asp Pro Ala Lys Leu Val

450 455 460

Glu Thr Ser Thr Asp Asp Ile Lys Asp Ser Gly Gly Glu Ser Phe Trp

465 470 475 480

Val Asp Lys His Lys Lys Ser Trp Ala Asp Met Ala Glu Glu Glu Glu

485 490 495

Glu Gln Glu Pro Thr Asp Pro Pro Val Gln His Leu Trp Asn Asp Ser

500 505 510

Pro Gln Lys Pro Pro Pro Phe Arg Thr Pro Thr Arg Ser Phe Arg Ser

515 520 525

Glu Glu Phe Asn Asp Glu Asn Leu Asp Ile Asn Ile Gly Gly His Glu

530 535 540

Arg Thr Leu Thr Glu Asn Leu Ser Arg Thr Leu Thr Phe Ser Asp Leu

545 550 555 560

Gly Cys Gly Ser Lys Ser Gln Thr Met Asp Thr Pro Ser Arg Thr Ser

565 570 575

Gly Arg Ser Ala Val Arg Arg Ser Leu Asn Phe Asn Gln Ile Pro Lys

580 585 590

Gln Glu Leu Asp Ser Val Met Leu Leu Glu Pro Glu Lys Ala Gly Gly

595 600 605

Cys Gly Ala Pro Glu Asp Ala Val Pro Val Lys Lys Ala Gly Gln Pro

610 615 620

Pro Arg Arg Lys Arg Leu Gln Val Phe Gln Asp Ile Thr Arg Leu Pro

625 630 635 640

Pro His Thr Ala

<210> 16

<211> 634

<212> PRT

<213> 桉树（Eucalyptus grandis）

<400> 16

Met Trp Gly Asn Arg Glu Asn Phe Pro Ala Arg Gly Tyr Phe Thr Pro

1 5 10 15

Gln Pro Pro Lys Arg Ala Thr Arg Pro Leu Val Ser Pro Val Leu Met

20 25 30

Ser Glu Arg Lys Arg Ala Ser Pro Pro Asp Leu Phe His Ile Ile His

35 40 45

Lys Val Pro Ala Gly Asp Ser Pro Tyr Val Lys Ala Lys Arg Val Gln

50 55 60

Leu Ile Asp Lys Asp Pro Ser Lys Ala Ile Ser Leu Phe Trp Ala Ala

65 70 75 80

Ile Asn Ala Gly Asp Arg Val Asp Ser Ala Leu Lys Asp Met Ala Ile

85 90 95

Val Met Lys Gln Leu Asn Arg Ser Asp Glu Ala Ile Glu Ala Ile Lys

100 105 110

Ser Phe Arg His Leu Cys Pro Phe Asp Ser Gln Glu Ser Leu Asp Asn

115 120 125

Val Leu Ile Glu Leu Tyr Lys Arg Ser Gly Arg Ile Glu Glu Glu Ile

130 135 140

Glu Met Leu Gln His Lys Leu Arg Leu Ile Glu Glu Gly Lys Gly Phe

145 150 155 160

Val Ala Asn Arg Thr Lys Thr Ala Arg Ser Gln Gly Lys Lys Ile Gln

165 170 175

Val Thr Arg Glu Gln Glu Arg Ser Arg Ile Met Gly Asn Leu Ala Trp

180 185 190

Ala His Leu Gln Leu Ser Asn Tyr Glu Ile Ala Glu Gly Leu Tyr Arg

195 200 205

Glu Ala Leu Ser Leu Glu Pro Asp Lys Asn Lys Gln Cys Asn Leu Ala

210 215 220

Ile Cys Leu Met Asn Met Asn Lys Leu Ala Asp Ala Lys Ala Val Leu

225 230 235 240

Asp Ala Val Arg Gly Pro Cys Gly Asp Gly Asp Met Asp Glu Ser Tyr

245 250 255

Ala Lys Ser Phe Asp Arg Ala Leu Gln Met Leu Asn Glu Ile Lys Ser

260 265 270

Leu Pro Ile Lys Gln Asn Glu Val Asp His Lys Glu Ile Gln Asn Ser

275 280 285

Arg Glu Ser Thr Cys Cys Thr Glu Arg Val Ile Asn Leu Gly Ala Gln

290 295 300

Pro Leu Ala Ser Thr Thr Ile Ala Asn Gly His Gly Glu Glu Thr Trp

305 310 315 320

Ile Leu Asn Glu Arg Asn Gly Met Leu Ser Arg Ala Glu Ala Trp Ser

325 330 335

Ser Ser Glu Asn Ile Phe Gly Asp Asn Trp Arg Glu Val Ser Asn Phe

340 345 350

Gly Thr Pro Leu Arg Tyr Val Leu Pro Gly Asn Leu His Ala Lys Glu

355 360 365

Asn Cys His Glu Thr Ser Lys Val Gly Trp Ser Ser Ser Ser Arg Ser

370 375 380

Ala Tyr Ala Ser Pro Ala Ser Val Lys Arg Asn Ile Glu Phe Ser Pro

385 390 395 400

Gly Glu Ser Arg Arg His Thr Cys Arg Ser Leu Ile Gln Val Glu Gly

405 410 415

Tyr Phe Asn Trp Glu Arg Gln Asn Ser Ser Glu Met Gly Ser Val Ala

420 425 430

Met Ala Lys Pro Lys Asn Tyr Asp Glu Asn Met Ile Arg Ser Lys Gly

435 440 445

Asp Pro Ala Lys Val Val Glu Thr Ser Thr Asp Asp Ile Lys Asp Ser

450 455 460

Gly Gly Glu Ser Phe Trp Val Asp Lys His Lys Lys Ser Trp Ala Asp

465 470 475 480

Met Ala Glu Glu Glu Glu Glu Gln Glu Pro Thr Asp Pro Pro Val Gln

485 490 495

His Leu Trp Asn Asp Ser Pro Gln Lys Pro Pro Pro Phe Arg Thr Pro

500 505 510

Thr Arg Ser Phe Arg Ser Glu Glu Phe Asn Asp Glu Asn Leu Asp Ile

515 520 525

Asn Ile Gly Gly His Glu Arg Thr Leu Thr Glu Asn Leu Ser Arg Thr

530 535 540

Leu Thr Phe Ser Asp Leu Gly Cys Gly Ser Lys Ser Gln Thr Met Asp

545 550 555 560

Thr Pro Ser Arg Thr Ser Gly Arg Ser Ala Val Arg Arg Ser Leu Asn

565 570 575

Phe Asn Gln Ile Pro Lys Gln Glu Leu Asp Ser Val Met Leu Leu Glu

580 585 590

Pro Glu Lys Ala Gly Gly Cys Gly Ala Pro Glu Asp Ala Val Pro Val

595 600 605

Lys Lys Ala Gly Gln Pro Pro Arg Arg Lys Arg Leu Gln Val Phe Gln

610 615 620

Asp Ile Thr Arg Leu Pro Pro His Thr Thr

625 630

<210> 17

<211> 689

<212> PRT

<213> 蓝花耧斗菜（Aquilegia caerula）

<400> 17

Met Gly Ile Asp Asn Lys Lys Val Gly Met Lys Gly Phe Ser Thr Pro

1 5 10 15

Pro Pro Pro Pro Leu Arg Trp Lys Ser Ala Pro Cys Thr Pro Ile Gln

20 25 30

Thr Leu Glu Met Asn Glu Glu Ser Leu His Gln Leu Ser Ala Thr Ile

35 40 45

Ala Lys Val Asp Ser Phe His Ile Ile His Lys Val Pro Ser Gly Asp

50 55 60

Ser Pro Tyr Val Lys Ala Lys His Val Gln Leu Ile Asp Lys Asp Pro

65 70 75 80

Ser Lys Ser Val Ala Leu Phe Trp Ser Ala Ile Asn Ala Gly Asp Arg

85 90 95

Val Asp Ser Ala Leu Lys Asp Met Ala Ile Val Met Lys Gln Leu Asp

100 105 110

Arg Ser Asp Glu Ala Ile Glu Ala Ile Lys Ser Phe Arg His Leu Cys

115 120 125

Thr Pro Leu Ser Gln Glu Ser Leu Asp Asn Val Leu Val Asp Leu Tyr

130 135 140

Lys Arg Gly Gly Arg Ile Glu Glu His Ile Glu Leu Leu His His Lys

145 150 155 160

Leu Lys Leu Ile Asp Asp Gly Val Ala Phe Gly Gly Lys Lys Thr Lys

165 170 175

Ile Ala Arg Ser Gln Gly Lys Lys Phe Leu Val Ser Ile Asp Gln Glu

180 185 190

Arg Ser Arg Leu Leu Gly Asn Leu Gly Trp Ala Tyr Met Gln Gln Asn

195 200 205

Asp Tyr Lys Thr Ala Glu Glu Ile Tyr Arg Lys Ala Leu Ser Ile Glu

210 215 220

Gln Asp Lys Asn Lys Gln Cys Asn Leu Ala Ile Cys Leu Met Asn Arg

225 230 235 240

Gly Glu Ile Met Glu Ala Lys Ser Leu Leu Gln Thr Val Thr Pro Ser

245 250 255

Ser Thr Glu Arg Glu Leu Val Asp Pro Phe Ile Lys Ser Phe Asp Arg

260 265 270

Ala Tyr Glu Met Leu Ile Glu Leu Glu Ser Lys Ser Cys Pro Asn Arg

275 280 285

Asn Ala Lys Pro Val Ala Ser Ala Ser Pro Ile Glu Glu Pro Leu Leu

290 295 300

Leu Lys Lys Gly Ser Tyr Val Cys Asp Asp Arg Leu Asp Ser Phe Gly

305 310 315 320

Lys Gly Arg Leu Asn Arg Glu Val Glu Met Glu Glu Lys Gly Gly Gly

325 330 335

His Ser Cys Arg Lys Ser Leu Phe Ala Asp Lys Gly Asn Lys Glu Asn

340 345 350

Cys Ser Pro Asn Leu Phe Glu Arg Gly Asn Gly Ile Ser Gly Trp Lys

355 360 365

Asn Val Glu Pro Phe Ser Glu Ser Arg Met Glu Glu Gly Ser Thr Arg

370 375 380

Lys Asn Val Arg Thr Phe Gly Phe His Arg Tyr Ser Asn Arg Ser Pro

385 390 395 400

Lys Leu Thr Thr Lys Tyr Gly Leu Gln Ser Pro Ser Asp Gly Asp Trp

405 410 415

Arg Arg Arg Ser Arg Asp Asn Asp Ala Asn Lys Asn Gln Ala Thr Ile

420 425 430

Thr Pro Thr Lys Ser Pro Ile Phe Ile Glu Ser Lys Cys Gly Leu Gln

435 440 445

Ser Thr Asp Gly Gly Cys Arg Lys Ser Pro Ile Ser Phe Ala Tyr Gln

450 455 460

Lys Ala His Glu Val Gly Gly Asp Leu Gln Ala Cys Ser Gly Gln His

465 470 475 480

Leu Glu Gln Arg Ser Glu Val Thr Val Thr Asp Ser Ala Ser Lys Tyr

485 490 495

Ile Gly Gly Asn Glu Thr Pro Glu Ala Cys Asn Gly Ile Asp Ser Thr

500 505 510

Thr Glu Gly Ser Thr Ser Lys Tyr Leu Cys Ser Ser Phe Arg Ser Gly

515 520 525

Lys Ser Trp Ala Asp Met Ala Glu Glu Glu Glu Glu Leu Leu Arg Glu

530 535 540

Cys Lys Asn Asn Ser Ile Ser Thr Trp Glu Tyr Pro Ser Phe Gln Thr

545 550 555 560

Pro Cys Lys Ser Val Asp Arg Trp Asn Asp Glu Lys Phe Phe His Asn

565 570 575

Glu Asn Met Asp Ser Asn Ile Ile Gly Ser Thr Pro Glu Leu Thr Thr

580 585 590

Gln Pro Arg Arg Asp Gly Tyr Thr Gln Thr Leu Ile Arg Lys Leu Glu

595 600 605

Leu Ile Asp Leu Lys Lys Glu Glu His Ile Lys Thr Thr Ala Ser Tyr

610 615 620

Ser Phe Ser Asn Pro Thr Ala Arg Arg Thr Leu Ala Phe Glu Lys Thr

625 630 635 640

Asp Ser Ala Asp Tyr Phe Arg Pro Ser Pro Leu Cys Lys Gln Val Leu

645 650 655

Phe Asn Gly Glu Ser Asp Asn Thr Ala Gly His Gly Lys Asn Ser Met

660 665 670

Pro Val Gly Arg Arg Ser Arg Arg Leu Pro Val Phe Gln Glu Ile Thr

675 680 685

Pro

<210> 18

<211> 625

<212> PRT

<213> 菜豆（Phaseolus vulgaris）

<400> 18

Met Leu Phe Glu Arg Gly Ser Pro Ala Arg Cys Phe Val Thr Pro Pro

1 5 10 15

Pro Pro Ser Pro Trp Lys Thr Ile Ser Ser Arg Ser Pro Ser Val Pro

20 25 30

Phe Ser Glu Arg Lys Lys Ser Pro Asn Ser Ala Asn Lys Ser Asp Ile

35 40 45

Phe His Ile Ile His Lys Val Pro Ser Gly Asp Ser Pro Tyr Val Lys

50 55 60

Ala Lys Gln Val Gln Leu Val Asp Lys Asp Pro Gly Arg Ala Ile Ser

65 70 75 80

Leu Phe Trp Ala Ala Ile Asn Ala Gly Asp Arg Val Glu Ser Ala Leu

85 90 95

Lys Asp Met Ala Leu Val Met Lys Gln Leu Asn Arg Ser Asp Glu Ala

100 105 110

Ile Glu Ala Ile Arg Ser Phe Arg His Leu Cys Pro Ser Asp Ser Gln

115 120 125

Asp Ser Leu Asp Asn Ile Leu Val Glu Leu Phe Lys Arg Ser Gly Arg

130 135 140

Val Asp Glu Glu Ile Ser Met Leu His His Lys Leu Lys Leu Ile Glu

145 150 155 160

Asp Gly Ile Thr Phe Val Gly Arg Thr Thr Lys Gln Ala Arg Ser Gln

165 170 175

Gly Lys Lys Ile Gln Ile Thr Ala Glu Gln Glu Ile Ser Arg Ile Leu

180 185 190

Gly Asn Leu Ala Trp Ala Tyr Leu Gln Lys Glu Asp Tyr Lys Thr Ala

195 200 205

Glu Glu His Tyr Arg Arg Ala Leu Ser Phe Glu Val Asp Arg Asn Lys

210 215 220

Gln Cys Asn Leu Ala Ile Cys Leu Met His Met Asn Lys Ile Lys Glu

225 230 235 240

Ala Lys Phe Leu Leu Gln Ala Val Arg Thr Ala Thr Lys Asn Arg Lys

245 250 255

Met Asp Glu Ser Phe Val Lys Ser Phe Glu Arg Ala Ser Gln Met Leu

260 265 270

Val Glu Ile Glu Thr Ser Ser Ser Glu Asn Ala Ser Phe Ser Met Thr

275 280 285

Thr Ser Leu Pro Gln Cys Ser Ser Leu Gly Phe Glu Asn Ser Val Arg

290 295 300

Lys Ser Ser Asp Arg Val Lys Arg Thr Glu Asn Gln Ser Asp Lys Ser

305 310 315 320

Glu Gly Asp Val Ser His Ala Arg Arg Arg Leu Tyr Gln Ser Pro Asp

325 330 335

Pro Gly Arg Arg His Leu Asn Leu Tyr Val Pro Thr Lys Pro Lys Arg

340 345 350

Cys Ser Trp Gly Phe Asn Asn Gly Tyr Arg Arg Gly Asp Phe His Ser

355 360 365

Asp Ser Lys Pro Ser Ser Gly Thr Pro Pro Asn Glu Lys His Val Thr

370 375 380

Arg Thr Leu Asn Leu Arg Glu Ser Gly Leu Ser Ser Pro Ala Asn Glu

385 390 395 400

Lys Trp Arg Thr Ser Thr Leu Glu Asp Pro Ala Ile Arg Lys Asn Lys

405 410 415

Asp Thr Lys Val Ile Ile Ser Asp Leu Leu His Thr Leu Asn Thr Glu

420 425 430

Ala Val Val Glu Phe Thr Glu Lys Glu Lys Ser Ala Ala Gly Asp Ser

435 440 445

Ser His Arg Ser Ile Met Ser Glu Ser His Ala Met Val Glu Asn Gly

450 455 460

Thr Asp Asp Phe Ala Ser Gly Asn Gly Lys Pro His Glu Lys Lys Ser

465 470 475 480

Trp Ala Asp Ile Val Glu Glu Glu Gln Asn Glu Glu Asn Asp Phe Phe

485 490 495

Ser Gly Tyr Ile Asn Phe Asp Gly Glu Asn Gly Ala Glu Val Phe Asn

500 505 510

Asp Glu Asn Glu Asp Ser Asn Ile Met Tyr Gln Ser Pro Trp Pro Gln

515 520 525

Asn Gln Pro Glu Trp Ser Ser Lys Lys Leu Glu Ser Leu Glu Gln Lys

530 535 540

Asp Gly Tyr His Pro Ser Gly Ser Val Ile Leu Ser Arg Asn Pro Thr

545 550 555 560

Ala Arg Arg Ser Leu Cys Phe Asn Pro Glu Leu Ser Ser Glu Ser Ala

565 570 575

Tyr Ala Ile Arg Thr Ser Lys Ser Pro Lys Lys Ala Ser Asn Leu Glu

580 585 590

Asn Arg Asp Thr Leu Val Gly Glu Lys Lys Leu Pro Arg Lys Ser Arg

595 600 605

Leu Gln Val Phe Gln Asp Ile Thr Leu Phe Pro Glu Thr Pro Arg Phe

610 615 620

Ala

625

<210> 19

<211> 758

<212> PRT

<213> 柿树（Prunus persica）

<400> 19

Met Trp Thr Arg Asp Lys Ser Leu His Thr Arg Gly Phe Ser Thr Pro

1 5 10 15

Pro Pro Thr Trp Lys Ser Gly Pro Pro Asn Pro Pro Met Val Pro Met

20 25 30

Ser Glu Arg Lys Arg Val Ser Pro Ser Asp Gly Gly Asp Leu Phe His

35 40 45

Val Met His Lys Val Pro Val Gly Asp Ser Pro Tyr Val Arg Ala Lys

50 55 60

Gln Val Gln Leu Ile Glu Lys Asp Pro Ser Lys Ala Ile Ser Leu Phe

65 70 75 80

Trp Ala Ala Ile Asn Ala Gly Asp Arg Val Asp Ser Ala Leu Lys Asp

85 90 95

Met Ala Ile Val Met Lys Gln Leu Asn Arg Ser Glu Glu Ala Ile Glu

100 105 110

Ala Ile Lys Ser Phe Arg His Leu Cys Pro His Asp Ser Gln Glu Ser

115 120 125

Leu Asn Asn Val Leu Val Glu Leu Tyr Lys Arg Ala Gly Arg Ile Glu

130 135 140

Glu Glu Ile Glu Met Leu Gln Ser Lys Leu Lys His Ile Asp Glu Gly

145 150 155 160

Ile Ala Phe Gly Gly Arg Arg Thr Lys Thr Ala Arg Ser Gln Gly Lys

165 170 175

Lys Val Gln Ile Thr Val Glu Gln Glu Arg Ser Arg Ile Leu Gly Asn

180 185 190

Leu Ala Trp Ala Tyr Leu Gln Gln Gly Asn Tyr Lys Thr Ala Glu Glu

195 200 205

Tyr Tyr Met Lys Ser Leu Ser Leu Glu Leu Asp Lys Asn Lys Gln Cys

210 215 220

Asn Leu Ala Ile Cys Leu Met His Met Asn Arg Leu Ala Glu Ala Lys

225 230 235 240

Ser Leu Leu Gln Val Val Arg Ala Ser Ser Gly Asn Lys Pro Met Asp

245 250 255

Glu Ser Tyr Ala Lys Ser Phe Glu Arg Ala Ile Gln Met Leu Thr Glu

260 265 270

Leu Glu Ala Lys Ser Val Leu Arg Pro Ile Gln Leu Asp Glu Asn Cys

275 280 285

Cys Lys Glu Ile Ser Arg Phe Pro Ile Ser Pro Ile Asn Arg Asn Ser

290 295 300

Lys Gln Gly Asn Ser Leu Thr Asn Glu Gly Gln His Tyr Val Ser Gly

305 310 315 320

Cys Met Ile Ser Arg Arg Trp Ala Asp Gly His Glu Glu Glu Thr Val

325 330 335

Ser Val Asn Lys Trp Lys Lys Asp Cys Tyr Phe Lys Asn Ser Cys Glu

340 345 350

Gly Arg Ser Ser Phe Ser Ser Arg Met Lys Glu Asn Gln Gly Gly Ile

355 360 365

Val Gly Thr Glu Thr Thr Pro Tyr Ser Lys Thr Phe Phe Ser Pro Ala

370 375 380

Pro Asp Ile Trp Asn Arg Glu Val Leu Phe Thr Gln Pro Arg Arg Ser

385 390 395 400

Ser Arg Gly Phe Asn Asp Gly His Gln Thr Arg Glu Ile Trp Gly Arg

405 410 415

Gly Val Gly Ser Ser Asn Lys Lys Leu Ser Phe Glu Ser Cys Ser Arg

420 425 430

Thr Glu Asn Met Arg Ala His Val Val Arg Ser Leu Asn Glu Asp Leu

435 440 445

Leu Ala Ser Thr Thr Gly Lys Ser Glu Val Ala Phe Gln Asn Ser Val

450 455 460

Ser Ser Ile Ser Ser Pro Ile Ser Arg Asp Leu Arg Arg Arg Pro Gln

465 470 475 480

Lys Asp Ala Ala Val Arg Ser Val Leu Gln Pro Ile Ser Ser Gly Asn

485 490 495

Trp Lys Cys Thr Ser Arg Ala Asn Asp Gly Cys Phe Gln Leu Lys Asp

500 505 510

Glu Ala Val Val Val Ser Ser Ser Gln Asn Thr Val Asn Gly Asp Trp

515 520 525

Arg Arg Thr Ser Trp Glu Asn Asp Gly Met Lys Lys Ser Ala Glu Pro

530 535 540

Leu Met Val Gly Glu Asp Ala Lys Ala Leu Glu Ile Ser Thr Asp Gly

545 550 555 560

Gly Pro Asn Gln Ser Ser Asp Thr Thr Ala Phe Val Glu Asp Cys Phe

565 570 575

Gly Glu Asn Thr Ser Ser Lys Val Asp Asp Val His Gln Pro Ile Ala

580 585 590

Glu Asn Gln Lys Pro Ala Pro Asp Phe Ser Met Tyr Ser Lys Cys Lys

595 600 605

Lys Ser Trp Ala Asp Met Val Glu Glu Glu Glu Gln Glu Leu Leu Asn

610 615 620

Gly Arg Thr Glu Tyr Phe Asp Ser Trp Tyr Thr Glu Asp Gly Phe Asn

625 630 635 640

Asn Glu Asn Leu Asn Cys Asn Ile Thr Pro Glu Ser Pro Cys Leu Gln

645 650 655

Ser Gln Met Lys Ser Leu Gly Gln Lys Leu Gln Ser Thr Asp Leu Val

660 665 670

Asp Glu Tyr Val Ser Gly Asn Ala Ala Ser Ser Arg Asn Ser Thr Val

675 680 685

Arg Arg Ser Leu Cys Phe Gly Gln Gln Gln Glu Gln Glu Ser Val Asp

690 695 700

Tyr Ile Ser Ser Ser Pro Val Pro Lys Glu Ala Leu Asn Phe Glu Gly

705 710 715 720

Ser Asp Ser Val Gln Ala Asn Gly Lys Gly Ser Ile Tyr Gly Lys Asn

725 730 735

Ser Ser Phe Ser Arg Arg Lys Arg Leu Gln Ser Phe Gln Asp Ile Thr

740 745 750

Glu Phe Gln Asp Ser Pro

755

<210> 20

<211> 649

<212> PRT

<213> 雷蒙德氏棉（Gossypium raimondii）

<400> 20

Met Trp Ser Ser Asp Lys His Cys Pro Ala Arg Gly Phe Leu Thr Pro

1 5 10 15

Gln Pro Pro Ala Trp Lys Lys Val Gln Ser Thr Thr Val Leu Pro Met

20 25 30

Ser Glu Arg Lys Arg Ile Ser Pro Ala Asn Asn Gly Asp Cys Phe His

35 40 45

Val Ile His Lys Val Pro Ala Ser Asp Ser Pro Tyr Gly Arg Ala Lys

50 55 60

His Val Gln Leu Ile Asp Lys Asp Pro Ser Lys Ala Val Ser Leu Phe

65 70 75 80

Trp Ala Ala Ile Asn Ala Gly Asp Arg Val Asp Ser Ala Leu Lys Asp

85 90 95

Met Ala Val Val Met Lys Gln Leu Asn Arg Ser Asp Glu Ala Ile Glu

100 105 110

Ala Ile Lys Ser Phe Arg His Leu Cys Pro Tyr Asp Ser Gln Glu Ser

115 120 125

Leu Asp Asn Val Leu Val Glu Leu Tyr Lys Arg Ser Gly Arg Val Asp

130 135 140

Glu Glu Ile Glu Ile Leu Leu Asn Lys Leu Arg Asn Ile Glu Glu Gly

145 150 155 160

Thr Val Phe Gly Gly Lys Lys Thr Lys Ile Ala Arg Ser Gln Gly Lys

165 170 175

Lys Ile Gln Ile Thr Ile Glu Gln Glu Lys Ser Arg Ile Leu Gly Asn

180 185 190

Leu Ala Trp Ala Tyr Leu Gln Gln His Asn Tyr Gly Ile Ala Glu Gln

195 200 205

His Tyr Arg Lys Ala Leu Ser Leu Glu Pro Asp Lys Asn Lys Gln Cys

210 215 220

Asn Leu Ala Ile Cys Leu Met His Met Asn Arg Leu Gly Glu Ala Lys

225 230 235 240

Ser Leu Leu Gln Asp Val Lys Val Ser Ala Gly Thr Glu Glu Met Asp

245 250 255

Glu Ser Tyr Ser Lys Ser Tyr Glu Arg Ala Met Glu Ile Leu Met Gln

260 265 270

Val Glu Thr Gln Ser Lys Leu Glu Pro Ala Gly Ala Gln Glu Pro Glu

275 280 285

Lys Gly Asn Glu Thr Arg Arg Cys Leu Thr Ser Cys Arg Asp Arg Ser

290 295 300

Leu Lys Glu Ala Ser Val Phe Leu Pro Arg Asn Gly Asp Asn Ile Pro

305 310 315 320

Trp Cys Ile Glu Lys Asn Gly Asn Leu Ser Gly Tyr Asp Asp Thr Ser

325 330 335

Ser Ser Gln Cys Thr Pro Ile Gly Leu Lys Gly Ser Phe Gln Cys Ser

340 345 350

Pro Gln Thr Met Leu Ser Glu Lys Trp Arg Lys Gly Ser Tyr Phe Glu

355 360 365

Ser Pro Ser Glu Gly Ser Val Tyr Ser Ser Ser Lys Leu Lys Glu Ser

370 375 380

Trp Arg Tyr Ser Ala Gly Gln Glu Val Gly Ser Ala His Lys Asn Met

385 390 395 400

Tyr Ala Ser Leu Ala Ala Ser Arg Lys Asn Ser Glu Lys Val Leu Leu

405 410 415

Thr Gln Pro Arg Arg Cys Ser Trp Gly Phe Asn Thr Ala Asp Gln Arg

420 425 430

Arg Gly Gly Arg Trp Gly Glu Asp Thr Thr Val Arg Asn Ser Ile Arg

435 440 445

Lys Leu Ser Phe Glu Gln Thr Thr Thr Thr Glu Ser Val Pro Ser Pro

450 455 460

Ser Ile Gln Lys Leu Lys Glu Glu Pro Leu Ser Ser Ser Asn Ala Lys

465 470 475 480

Ser Glu Asn Tyr Ser Ala Val Gly Leu Gly Glu Glu Glu Ala Gln Glu

485 490 495

Gly Leu Ser Gly Val Leu Phe Thr Gln Pro Arg Asn Ser Leu Ser Trp

500 505 510

Leu Asn Asn Arg Asp Gln Arg Arg Gly Arg Cys Ala Glu Glu Ser Ile

515 520 525

Asp Gly Ser Phe Ser Lys Leu Ser Ser Ser Val Thr Thr His Ser Val

530 535 540

Gln Ser Leu Asn Val Glu Pro Leu Val Ser Ser Lys Asp Glu Ser Glu

545 550 555 560

Ile Gly Val Glu Lys Pro Ala Asp Ala Ala Ser Asn Lys Lys Thr Trp

565 570 575

Ala Asp Met Val Glu Glu Glu Glu Lys Asp Glu Phe Leu Asn Asp Glu

580 585 590

Asn Leu Asn Ser Asn Ile Ile Tyr Gln His Pro Asp Arg Ser Lys His

595 600 605

His Ile Glu Asn Ile Thr Gln Gln Leu Glu Ser Phe Gly Val Lys Gly

610 615 620

Gly Tyr Asn Ala Ser Ala Asn Thr Val Ser Leu Arg Arg Asn Arg Leu

625 630 635 640

Gln Val Phe Arg Asp Ile Thr Ser Thr

645

<210> 21

<211> 614

<212> PRT

<213> 番茄（Solanum lycopersicum）

<400> 21

Met Trp Arg Asn Asn Glu Arg Val Tyr Met Thr Pro Ala Arg Gly Phe

1 5 10 15

Leu Thr Pro Pro Pro Lys Trp Arg Ser Pro Ala Thr Glu Lys Asp Gln

20 25 30

Lys Trp Pro Thr His Ala Gln Ser Ala Lys Ala Asp Leu Phe His Val

35 40 45

Ile His Lys Val Pro Ser Gly Asp Ser Pro Tyr Val Arg Ala Lys His

50 55 60

Val Gln Leu Ile Asp Lys Asp Pro Gly Lys Ala Val Ser Leu Phe Trp

65 70 75 80

Ala Ala Ile Asn Ser Gly Asp Arg Val Asp Ser Ala Leu Lys Asp Met

85 90 95

Ala Val Val Met Lys Gln Leu Asp Arg Ser Asp Glu Ala Ile Glu Ala

100 105 110

Ile Lys Ser Phe Arg Asn Leu Cys Pro Ser Glu Ser Gln Glu Ser Ile

115 120 125

Asp Asn Ile Leu Ile Glu Leu Tyr Lys Arg Ser Gly Arg Leu Glu Glu

130 135 140

Glu Ile Glu Leu Leu Glu Leu Lys Leu Lys Asn Val Glu Glu Gly Ile

145 150 155 160

Ala Phe Gly Gly Lys Arg Thr Lys Ile Ala Arg Ser Gln Gly Lys Lys

165 170 175

Val Gln Ile Thr Ile Glu Lys Glu Tyr Ala Arg Leu Leu Gly Asn Leu

180 185 190

Ala Trp Ser Tyr Met Gln Leu Asn Asn Phe Lys Leu Ala Glu Glu Tyr

195 200 205

Tyr Arg Lys Ala Leu Ser Leu Glu Ser Asp Lys Asn Lys Gln Ser Asn

210 215 220

Leu Ala Ile Cys Leu Met His Met Asn Lys Ile Ala Glu Ala Arg Phe

225 230 235 240

Leu Leu Gln Ser Ile Lys Thr Ser Asp Arg Arg Gln Met Asp Glu Ser

245 250 255

Cys Thr Lys Ser Phe Glu Arg Ala Thr Gln Met Leu Ala Glu Leu Glu

260 265 270

Ser His Gly Ile Gln Asn Ser Lys Glu Gln Val Glu Glu Met Arg Glu

275 280 285

Val Arg Ile Asp Ser Ser Thr Ser Asp Glu His Asp Arg Arg Gly His

290 295 300

Glu Lys Thr His Pro Pro Pro Phe Thr Ala Ser Gly Pro Pro Lys His

305 310 315 320

Phe Leu Thr Gln Pro Arg Arg Tyr Ser Cys Ser Leu Asn Asp Gly Gly

325 330 335

Trp Leu Asn Lys Asp Ser Val Ser Ala Cys Ser Arg Arg Leu Leu Phe

340 345 350

Glu Gln Thr Ser Asn Asn Glu Asn Val Gln Leu Val Val Asn His Asn

355 360 365

Phe Asn Lys Leu Ile Ser Val Asn Asp Ile Ser Glu Gly Ala Ser Val

370 375 380

Val Cys Gly Gln Val Phe Ser Arg Ser Trp Gly Asn Gly Ala Asn Val

385 390 395 400

Arg Ser Glu Cys Asp Leu Gln Pro Pro Tyr Ser Lys Trp Lys Asn Asn

405 410 415

Ser Ser Gly Asn Asp Gly Ser Asp Gln Ile Ser Leu Glu Leu Ser Arg

420 425 430

Ser Pro Thr Glu Ser Leu Pro Asp Ile Thr Ser Ala Arg Lys Cys Ser

435 440 445

Glu Asn Gly Ser Lys Asp Cys Trp Ser Ser Thr Leu Thr Tyr Arg Asp

450 455 460

Met Val Thr Leu Glu Asp Thr Thr Glu His Leu Asp Ser Thr Ser Leu

465 470 475 480

Lys Pro Leu Asn Leu Pro Ala Cys Thr Ser Lys Lys Ser Trp Ala Asp

485 490 495

Met Val Glu Glu Asp Glu Leu Gly Leu Gln Phe His Glu Thr Pro Gly

500 505 510

Lys Tyr Ser Asp Glu Asn Glu Asn Ile Asp Ala Asn Ile Ile Asn Leu

515 520 525

Ser Gln Asn Ile Asp Thr Leu Cys Leu Asn Glu Gly Tyr His Thr Gln

530 535 540

Pro Gly Arg Glu Ala Arg Arg Ser Leu Cys Phe Asp His Asn Asp Arg

545 550 555 560

Lys Glu Lys Cys Ser Ser Gly Phe Gln Gly Lys Glu Leu Lys Ser Gly

565 570 575

Ser Leu Asn Ser Leu Pro Pro Ile Gly Asp Ile Ala Tyr Gln Thr Pro

580 585 590

Val Thr Leu Met Arg Arg Asn Arg Leu Gln Val Phe Arg Asp Ile Thr

595 600 605

Pro Glu Ser Pro Lys Pro

610

<210> 22

<211> 614

<212> PRT

<213> 马铃薯（Solanum tuberosum）

<400> 22

Met Trp Arg Asn Asn Glu Arg Val Tyr Met Ser Pro Ala Arg Gly Phe

1 5 10 15

Leu Thr Pro Pro Pro Lys Trp Arg Ser Pro Ala Ser Asp Lys Asp Gln

20 25 30

Arg Trp Pro Thr His Ala Gln Ser Ala Lys Ala Asp Leu Phe His Val

35 40 45

Ile His Lys Val Pro Ser Gly Asp Ser Pro Tyr Val Arg Ala Lys His

50 55 60

Val Gln Leu Ile Asp Lys Asp Pro Gly Lys Ala Ile Ser Leu Phe Trp

65 70 75 80

Ala Ala Ile Asn Ser Gly Asp Arg Val Asp Ser Ala Leu Lys Asp Met

85 90 95

Ala Val Val Met Lys Gln Leu Asp Arg Ser Asp Glu Ala Ile Glu Ala

100 105 110

Ile Lys Ser Phe Arg Asn Leu Cys Pro Ser Glu Ser Gln Glu Ser Ile

115 120 125

Asp Asn Ile Leu Ile Glu Leu Tyr Lys Arg Ser Gly Arg Leu Glu Glu

130 135 140

Glu Ile Glu Leu Leu Glu Leu Lys Leu Lys Asn Val Glu Glu Gly Ile

145 150 155 160

Ala Phe Gly Gly Lys Arg Thr Lys Ile Ala Arg Ser Gln Gly Lys Lys

165 170 175

Val Gln Ile Thr Ile Glu Lys Glu Tyr Ala Arg Leu Leu Gly Asn Leu

180 185 190

Ala Trp Ser Tyr Met Gln Leu Asn Asn Phe Lys Leu Ala Glu Glu Tyr

195 200 205

Tyr Arg Lys Ala Leu Ser Leu Glu Ser Asp Lys Asn Lys Gln Ser Asn

210 215 220

Leu Ala Ile Cys Leu Met His Met Asn Lys Ile Ala Glu Ala Arg Phe

225 230 235 240

Leu Leu Gln Ser Ile Lys Ala Ser Asp Arg Trp Gln Met Asp Glu Ser

245 250 255

Cys Thr Lys Ser Phe Glu Arg Ala Thr Gln Met Leu Ala Glu Leu Glu

260 265 270

Thr His Gly Ile Gln Asn Ser Lys Glu Gln Val Glu Glu Met Arg Glu

275 280 285

Val Arg Ile Asp Ser Ser Thr Ser Asp Glu His Asp Cys Arg Gly His

290 295 300

Glu Lys Thr His Pro Pro Pro Phe Thr Ala Ser Gly Pro Pro Lys His

305 310 315 320

Phe Leu Thr Gln Pro Arg Arg Tyr Ser Cys Ser Leu Asn Asp Gly Gly

325 330 335

Trp Leu Asn Lys Asp Ser Val Ser Ala Cys Ser Arg Arg Leu Leu Phe

340 345 350

Glu Gln Thr Ser Asn Asn Glu Asn Val Gln Leu Val Val Asn His Asn

355 360 365

Phe Asn Lys Leu Ile Ser Ala Asn Asp Met Ser Glu Gly Ala Ser Leu

370 375 380

Val Arg Gly Gln Val Leu Thr Arg Ser Trp Gly Asn Gly Ala Asn Val

385 390 395 400

Glu Ser Glu Cys Asp Leu Gln Pro Pro Tyr Ser Lys Trp Lys Asn Asn

405 410 415

Ser Ser Gly Asn Asp Gly Ser Asp Gln Ile Ser Leu Glu Leu Ser Lys

420 425 430

Ser Pro Thr Glu Ser Leu Ala Asp Ile Thr Ser Ala Arg Lys Tyr Ser

435 440 445

Gly Asp Gly Ser Lys Asp Cys Trp Ser Ser Thr Leu Thr Tyr Arg Asp

450 455 460

Thr Val Thr Leu Glu Asp Thr Thr Glu His Leu Glu Ser Thr Asn Leu

465 470 475 480

Lys Pro Leu Asn Leu Pro Ala Cys Thr Ser Lys Lys Ser Trp Ala Asp

485 490 495

Met Val Glu Glu Asp Glu Leu Gly Leu Gln Phe His Glu Thr Pro Gly

500 505 510

Lys Tyr Ser Asp Glu Asn Glu Asn Ile Asp Ser Asn Ile Ile Asn Leu

515 520 525

Ser Gln Asn Ile Asp Thr Leu Arg Leu Asn Glu Gly Tyr His Thr Gln

530 535 540

Pro Gly Arg Glu Ala Arg Arg Ser Leu Cys Phe Asp Gln Asn Asp Arg

545 550 555 560

Lys Glu Lys Cys Ser Ser Asp Phe Gln Gly Lys Val Leu Lys Ser Gly

565 570 575

Ser Leu Asn Ser Leu Pro Pro Ile Gly Asp Ile Ala Tyr Gln Thr Pro

580 585 590

Val Thr Leu Met Arg Arg Asn Arg Leu Gln Val Phe Arg Asp Ile Thr

595 600 605

Pro Glu Ser Pro Lys Pro

610

<210> 23

<211> 683

<212> PRT

<213> 粟（Setaria italica）

<400> 23

Met Pro Ser Gly Gly Arg Arg Leu Pro Pro Trp Thr Ser Pro Arg Ser

1 5 10 15

Ala Gly Ala Pro Arg Trp Ser Pro Ala Ala Gly Thr Pro Val Ala Gly

20 25 30

Ala Gly Cys Gly Pro Val Ser Gly Tyr Arg Thr Pro Pro Val Ser Ala

35 40 45

Gly Gly Cys Phe Gly Thr Arg Val Thr Pro Pro Thr Ser Gly Gly Ala

50 55 60

Arg Val Thr Pro Pro Ser Thr Gly Gly Cys Ser Ser Arg Pro Pro Arg

65 70 75 80

Pro Pro Pro Ser Leu Asp Ser Pro Tyr Val Arg Ala Lys Gln Ala Gln

85 90 95

Val Ile Glu Lys Asp Pro Asn Lys Ala Val Pro Leu Phe Trp Ala Ala

100 105 110

Ile Asn Ser Gly Asp Arg Ile Glu Ser Ala Leu Lys Asp Met Ala Asn

115 120 125

Val Leu Lys Gln Ala Asn Arg Ala Glu Glu Ala Ile Glu Ala Ile Arg

130 135 140

Ser Phe Arg Asp Arg Cys Pro Tyr Glu Ala Gln Asp Ser Leu Asp Asn

145 150 155 160

Ile Leu Leu Asp Leu Tyr Lys Lys Cys Gly Arg Thr Glu Glu Gln Ile

165 170 175

Glu Met Leu Thr Ile Lys Leu Arg Val Val Asp Glu Glu Leu Ala Ser

180 185 190

Gly Arg Trp Lys Thr Lys Leu Ser Lys Ser His Gly Arg Val Val Tyr

195 200 205

Leu Ser Leu Arg Asp Glu Lys Ala Arg Leu Leu Gly Asn Leu Ala Trp

210 215 220

Ala Tyr Met Gln Ser Glu Asn Tyr Glu Glu Ala Glu Met Leu Tyr Arg

225 230 235 240

Gln Ala Leu Ala Ile Glu Ala Asp Tyr Asn Lys Glu Cys Asn Leu Ala

245 250 255

Ile Cys Leu Met Lys Thr Gly Lys Leu Ala Glu Ala Lys Tyr Leu Leu

260 265 270

Gln Ala Ile Pro Tyr Asn Cys Asp Asp Glu Ser His Val Lys Ser Leu

275 280 285

Ser Arg Ala Thr Glu Met Leu Arg Asp Leu Glu Leu Gln Ser Leu Pro

290 295 300

Ser Pro Ile Thr Gln Met Lys Ser Lys Glu Ser Arg Ile Leu Leu Ala

305 310 315 320

Thr Asp Val Glu Ile Leu Glu Asp Pro Gln Pro Gln Thr Leu Ser Thr

325 330 335

Pro Leu Ser Gln Leu Lys Tyr Lys Glu Pro His Ile Ser Val Ser Ala

340 345 350

Asn Ala Glu Gln His Glu Lys Cys Ser Ser Trp Phe Pro Ser Pro Ile

355 360 365

Thr Gln Leu Lys Arg Glu Glu Pro Arg Ile Leu Val Thr Val Asp Ala

370 375 380

Glu Lys Asn Glu Gly Cys Ala Glu Phe Gln Asp Leu Ser Arg Leu Phe

385 390 395 400

Asn Asp Ala Ala Thr Pro His Ser Ile Leu Glu Lys Leu Arg Lys Arg

405 410 415

Leu Val Asn Glu Ala Pro Lys Ser Ser Ile His Asp Gln Ile Gln Thr

420 425 430

His Thr Pro Thr Glu Cys Leu Pro Asn Ser Glu Gly Asn His Asn Ala

435 440 445

Ser Glu Asn Pro Val Gln Gly Gly Lys Leu Leu Thr Lys Gly Val Arg

450 455 460

Lys Thr Trp Ala Asp Met Val Asp Glu Glu Glu Gln Gln Leu Gly Glu

465 470 475 480

Asp Lys Ser Trp Thr Asp Met Val Ala Lys Gly Glu His Gln Leu Arg

485 490 495

Asn Asp Lys Leu Thr Val Gly Val Gly Thr Thr Glu Gln Thr Glu Ser

500 505 510

Ser Lys His Ala Ser Lys Gln Glu Tyr Arg Thr Pro Pro Pro Ser Gln

515 520 525

Gly Ser Ser Thr Leu His Arg Pro Val Ile Gly Gly His Gln Gln Gly

530 535 540

Phe Ser Ala Asn Ser Trp Arg Arg Ser Asn Ser Lys Ile Ser Thr Asp

545 550 555 560

Asn Lys Val Asn Trp Asp Leu Val Arg Ala Ala Pro Thr Trp Ser Lys

565 570 575

His Lys Val Gln Asp His Ser Gly Arg Val Cys Gln Arg Pro Asn Ala

580 585 590

Ala His Leu Lys Glu Asn Thr Ser Gly Ser Lys Gln Ala Pro Trp Arg

595 600 605

Ser Ser Ala Ser Gln Arg Ala Leu Phe Pro Asp Trp Lys Ser Lys Gly

610 615 620

Glu Gly Tyr Gly His Gly Tyr Val Pro Phe Gly Asp Asn Glu His Ser

625 630 635 640

Gln Gly Ser Ser Arg Thr Glu Ala Thr His Arg Trp His Asn Asn Ala

645 650 655

Ala Gly Thr Val Ser Trp Arg Pro Gln Asn Arg Leu Arg Val Phe Gln

660 665 670

Glu Ile Thr Asn Glu Ile Asn Gln Asn Val Val

675 680

<210> 24

<211> 717

<212> PRT

<213> 二穗短柄草（Brachypodium distachyon)

<400> 24

Met Arg Ser Gly Gly Arg Arg Leu Pro Pro Trp Thr Ser Pro Arg Gly

1 5 10 15

His Ala Ala Ala Glu Val Val Ala Pro Thr Gly Trp Ser Pro Arg Thr

20 25 30

Pro Ala Ala Gly Gly Gly Gly Ser Gly Ser Tyr Val Thr Pro Pro Leu

35 40 45

Thr Ala Gly Cys Cys Cys Ser Ser Ser Ser Tyr Arg Val Thr Pro Pro

50 55 60

Ser Ser Gly Gly Gly Cys Thr Ser Leu Leu Thr Pro Pro Val Ser Gly

65 70 75 80

Gly Gly Cys Ser Arg Pro Pro Arg Ala Pro Pro Ala Val Val Asp Ser

85 90 95

Pro Tyr Val Arg Ala Lys Gln Ala Gln Leu Ile Glu Lys Asp Pro Asn

100 105 110

Lys Ala Val Pro Leu Phe Trp Ala Ala Ile Asn Ser Gly Glu Arg Ile

115 120 125

Glu Ser Ala Leu Lys Asp Met Ala Thr Val Leu Lys Gln Ala Asn Arg

130 135 140

Ala Glu Glu Ala Ile Glu Ala Ile Arg Ser Phe Arg Asp Arg Cys Pro

145 150 155 160

Asn Glu Ala Gln Asp Ser Leu Asp Asn Val Leu Leu Asp Leu Tyr Lys

165 170 175

Lys Cys Gly Arg Thr Lys Glu Gln Ile Glu Met Leu Thr Ile Lys Leu

180 185 190

Arg Met Val Asp Glu Asp Leu Ala Ser Gly Arg Trp Lys Thr Lys Leu

195 200 205

Ser Lys Ser His Gly Arg Val Val Tyr Leu Ser Leu Arg Asp Glu Lys

210 215 220

Ala Arg Leu Leu Gly Asn Leu Ala Trp Ala His Met Gln Ser Glu Asn

225 230 235 240

Tyr Glu Glu Ala Glu Met Leu Tyr Arg Gln Ala Leu Ala Ile Glu Ala

245 250 255

Asp Tyr Asn Lys Glu Cys Asn Leu Ala Val Cys Leu Met Lys Thr Gly

260 265 270

Lys Val Ala Glu Ala Lys Tyr Leu Leu Gln Ala Ile Pro Tyr Asn Ser

275 280 285

Ser Asp Glu Lys His Val Arg Ser Phe Ala Arg Ala Thr Glu Met Ile

290 295 300

Lys Glu Leu Glu Ser Gln Ala Leu Pro Ser Pro Ile Thr Gln Met Lys

305 310 315 320

Ser Lys Asp Ser Arg Ile Leu Leu Ala Thr Asp Ala Glu Asn Leu Asp

325 330 335

Tyr Ile Gln Pro Glu Ile Leu Ser Thr Ser Ser Thr Gln Leu Gln Tyr

340 345 350

Glu Glu Pro Glu Phe Ser Val Ser Ala Asp Thr Glu Lys Gln Val Asp

355 360 365

Cys Asn Ser Gln Glu Leu Pro Ser Pro Ile Thr Gln Leu Lys Arg Lys

370 375 380

Val Pro Gln Ile Met Val Asp Ser Glu Lys Asn Gly Glu Cys Pro Glu

385 390 395 400

Glu Asn Gln Asp Ile Ser Arg Leu Phe Asn Asp Ala Ala Thr Pro Gln

405 410 415

Ser Leu Leu Glu Lys Leu Arg Lys Arg Leu Val Lys Lys Asp Arg Pro

420 425 430

Asn Ile Ser Ile Gln His Gln Ala Gln Thr Pro Ser Ser Thr Glu Cys

435 440 445

Leu Pro Ile Cys Asn Gly Ala Thr Asp Ala Ser Asp Asn His Leu Gln

450 455 460

Glu Gly Gln Ser Ser Val Gly Gly Ala Arg Lys Thr Trp Ala Asp Met

465 470 475 480

Val Glu Glu Asp Glu Gln Gln Leu Gly Asp Gly Lys Ser Gly Thr Ala

485 490 495

Gln Asn Glu Ser Ser Lys Gln Ala Ser Glu Gln Arg Tyr Ile Thr Pro

500 505 510

Pro Ser Ser Gln Ala Asn Ser Thr Leu Gln Thr Pro Ala Ala Gly Val

515 520 525

Arg Leu Gln Ser Ser Ser Ala Gly Ser Trp Arg Arg Lys Asp Ser Thr

530 535 540

Gly Glu Asn Val Asn Arg Lys Phe Val Arg Thr Ala Pro Ala Trp Arg

545 550 555 560

Gln Gln Lys Val Gln Asp His Ser Asn Arg Val Cys Gln Arg Leu Asn

565 570 575

Thr Ile His Leu Gly Glu Lys Ala Gln Gly Gln Gly Ala Glu Gln Thr

580 585 590

Pro Trp Arg Ser Ser Ala Ala Gln Arg Ser Leu Phe His Gly His Leu

595 600 605

Pro Ser Gly Glu Ser Gly Arg Cys His Val Ser Gly His Thr Glu Ala

610 615 620

Val Asn Arg Trp Pro Lys Asn Ala Ala Ala Ile Thr Arg Pro Trp Arg

625 630 635 640

Ala Gln Asn Arg Leu Arg Val Phe Gln Glu Ile Thr Asn Glu Met Asn

645 650 655

Arg Asn Val Ala Tyr Glu His Cys Cys Leu Pro Cys Met Gln Thr Leu

660 665 670

Cys Ala Cys Thr Phe Trp Arg Tyr Cys Leu Asp Val Thr Ala Trp Phe

675 680 685

Ile Cys Cys Asn Val Leu His Val Ala Phe Ile Cys Tyr Cys Cys Cys

690 695 700

Cys Trp Leu Ala Gly Trp Leu Ala Gly Gln Phe Pro Ala

705 710 715

<210> 25

<211> 815

<212> PRT

<213> 粳稻（Oryza sativa japonica）

<400> 25

Met Pro Ser Gly Gly Arg Arg Leu Pro Pro Trp Thr Ser Pro Arg Gly

1 5 10 15

Ala Ala Pro Arg Trp Ser Pro Cys Thr Pro Ala Gly Ala Asp Gly Ser

20 25 30

Gly Arg Ala Ala His Ala Thr Pro Pro Ala Ser Gly Gly Cys Ser Ser

35 40 45

His Val Thr Pro Pro Ala Ser Gly Gly Gly Gly Cys Tyr Gly Tyr Arg

50 55 60

Val Thr Pro Pro Thr Ser Gly Gly Cys Ser Arg Pro Pro Arg Ala Pro

65 70 75 80

Leu Ser Ser Val Asp Ser Pro Tyr Val Arg Ala Lys Gln Ala Gln Val

85 90 95

Ile Glu Lys Asp Pro Asn Lys Ala Val Pro Leu Phe Trp Ala Ala Ile

100 105 110

Asn Ser Gly Asp Arg Ile Glu Ser Ala Leu Lys Asp Met Ala Thr Val

115 120 125

Leu Lys Gln Ala Asn Arg Ala Glu Glu Ala Ile Glu Ala Ile Arg Ser

130 135 140

Phe Arg Asp Arg Cys Pro Asn Glu Ala Gln Glu Ser Leu Asp Asn Ile

145 150 155 160

Leu Leu Asp Leu Tyr Lys Lys Cys Gly Arg Thr Lys Glu Gln Ile Glu

165 170 175

Met Leu Thr Leu Lys Leu Arg Ile Val Asp Glu Glu Leu Ala Ser Gly

180 185 190

Arg Trp Lys Thr Lys Leu Ser Lys Ser His Gly Arg Val Val Tyr Leu

195 200 205

Ser Leu Arg Asp Glu Lys Ala Arg Leu Leu Gly Asn Leu Ala Trp Ala

210 215 220

His Met Gln Ser Glu Asn Tyr Asp Glu Ala Glu Met Leu Tyr Arg Gln

225 230 235 240

Ala Leu Ala Ile Glu Ala Asp Tyr Asn Lys Glu Cys Asn Leu Ala Ile

245 250 255

Cys Leu Ile Lys Thr Gly Lys Val Ala Glu Ala Lys Tyr Leu Leu Gln

260 265 270

Ser Ile Pro Asp Asn Cys Ser Asp Glu Ser His Val Arg Ser Leu Ala

275 280 285

Arg Ala Arg Glu Met Leu Met Glu Leu Glu Ser Pro Thr Leu His Ser

290 295 300

Pro Ile Thr Gln Met Lys Ser Lys Glu Ser Leu Ile Trp Leu Ala Ile

305 310 315 320

Asp Ala Glu Asn Leu Gly His Leu Gln Pro Gln Val Ser Ser Thr Ala

325 330 335

Leu Thr Gln Leu Lys Ser Glu Glu Pro His Ile Ser Val Ala Ala Asp

340 345 350

Ala Glu Lys Gln Glu Asp Cys Asn Ser Gln Val Phe Pro Ser Pro Ile

355 360 365

Thr Gln Met Lys Arg Glu Glu Pro Glu Ser Leu Ile Ala Thr Ser Gly

370 375 380

Glu Asn Asn Glu Lys Cys Leu Asn Glu Tyr Gln Asp Leu Ser Arg Leu

385 390 395 400

Phe Asn Asp Ala Ala Thr Pro Gln Ser Leu Leu Glu Lys Leu Arg Lys

405 410 415

Arg Leu Val Lys Glu Asp Thr Leu Asn Ile Ser Ile Gln His Gln Val

420 425 430

Gln Ile Pro Ser Phe Val Glu Cys Leu Pro Asn Ser Gly Gly Ser Thr

435 440 445

Asp Ala Gly Glu Asn Thr Arg Pro Glu Gly Lys Ala Leu Val Asn Gly

450 455 460

Val Arg Lys Thr Trp Ala Asp Met Val Glu Glu Asp Glu Arg Gln Leu

465 470 475 480

Gly Asp Val Ser Ser Thr Ile Gly Met Asp Thr Thr Lys Arg Asn Val

485 490 495

Ser Cys Lys His Ala Asn Glu Glu Met Tyr Arg Thr Pro Ser Phe Ser

500 505 510

Gln Glu Ser Ser Ala Leu Lys Arg Ser Ser Val Asp Asp His Pro Gln

515 520 525

Ser Ser Ser Ala Asp Ser Trp Arg His Ser Asp Ser Lys Ile Ser Thr

530 535 540

Asp Glu Asn Val Asn Met Lys Phe Val Arg Thr Ala Pro Gln Trp Arg

545 550 555 560

Gln Gln Lys Val Gln Asp Tyr Ser Asn Arg Val Ser Gln Arg Leu Asp

565 570 575

Thr Ser His Leu Ser Asp Arg Ala Glu Gly Thr Glu Gln Pro Pro Trp

580 585 590

Arg Ser Ser Thr Ala Gln Arg Ser Leu Phe Pro Asp Trp Lys Ser Lys

595 600 605

Cys Glu Arg Tyr Gly His Gly Tyr Val Pro Phe Gly Asp Asn Glu His

610 615 620

Phe Gln Gly Ser Ser His Phe Glu Ala Thr Asn Arg Trp Pro Lys Asn

625 630 635 640

Ala Arg Pro Trp Arg Pro Gln Asn Arg Leu Trp Val Phe Gln Glu Ile

645 650 655

Thr Asn Glu Ile Asn Gln Lys Gln Thr Arg Ala Leu Trp Leu Thr Ile

660 665 670

Tyr Thr Thr Leu Leu Phe Gly Thr Thr Ala Leu Asp Asp Thr Ala Trp

675 680 685

Phe Leu Val Glu Cys Phe His Leu Leu Ala Gly His Leu Leu Leu His

690 695 700

Asp Gly Leu Gln Asp Leu Ser Val Ala Pro Pro Ser Gly Gln Phe Ala

705 710 715 720

Glu Met Ala Met Gln Met His Pro Ala Thr Gly Leu Ser Thr His Trp

725 730 735

Ser Gln Val Asp Ser Pro Pro Leu Lys Leu Arg Glu Gly Ala Asn Met

740 745 750

Val Val Leu Leu Gln Asp Gln Leu Leu Pro Met Ala Phe Ser Ile Ala

755 760 765

Asn Val Cys Glu Asp Cys Ile Met Ala Tyr Met Ser Ala Ser Ala Ser

770 775 780

Ser Val Leu Phe Tyr Thr Ser Cys Leu Asp Met Gln Gln Leu Pro Tyr

785 790 795 800

Thr Gly His Val Ile Thr Ile Asn Lys Ser Glu Ala Ala Thr Ile

805 810 815

<210> 26

<211> 813

<212> PRT

<213> 籼稻（Oryza sativa indica）

<400> 26

Met Pro Ser Gly Gly Arg Arg Leu Pro Pro Trp Thr Ser Pro Arg Gly

1 5 10 15

Ala Ala Pro Arg Trp Ser Pro Cys Thr Pro Ala Gly Ala Asp Gly Ser

20 25 30

Gly Arg Ala Ala His Ala Thr Pro Pro Ala Ser Gly Gly Cys Ser Ser

35 40 45

His Val Thr Pro Pro Ala Ser Gly Gly Gly Gly Cys Tyr Gly Tyr Arg

50 55 60

Val Thr Pro Pro Thr Ser Gly Gly Cys Ser Arg Pro Pro Arg Ala Pro

65 70 75 80

Leu Ser Ser Val Asp Ser Pro Tyr Val Arg Ala Lys Gln Ala Gln Val

85 90 95

Ile Glu Lys Asp Pro Asn Lys Ala Val Pro Leu Phe Trp Ala Ala Ile

100 105 110

Asn Ser Gly Asp Arg Ile Glu Ser Ala Leu Lys Asp Met Ala Thr Val

115 120 125

Leu Lys Gln Ala Asn Arg Ala Glu Glu Ala Ile Glu Ala Ile Arg Ser

130 135 140

Phe Arg Asp Arg Cys Pro Asn Glu Ala Gln Glu Ser Leu Asp Asn Ile

145 150 155 160

Leu Leu Asp Leu Tyr Lys Lys Cys Gly Arg Thr Lys Glu Gln Ile Glu

165 170 175

Met Leu Thr Leu Lys Leu Arg Ile Val Asp Glu Glu Leu Ala Ser Gly

180 185 190

Arg Trp Lys Thr Lys Leu Ser Lys Ser His Gly Arg Val Val Tyr Leu

195 200 205

Ser Leu Arg Asp Glu Lys Ala Arg Leu Leu Gly Asn Leu Ala Trp Ala

210 215 220

His Met Gln Ser Glu Asn Tyr Asp Glu Ala Glu Met Leu Tyr Arg Gln

225 230 235 240

Ala Leu Ala Ile Glu Ala Asp Tyr Asn Lys Glu Cys Asn Leu Ala Ile

245 250 255

Cys Leu Ile Lys Thr Gly Lys Val Ala Glu Ala Lys Tyr Leu Leu Gln

260 265 270

Ser Ile Pro Asp Asn Cys Ser Asp Glu Ser His Val Arg Ser Leu Ala

275 280 285

Arg Ala Arg Glu Met Leu Met Glu Leu Glu Ser Pro Thr Leu His Ser

290 295 300

Pro Ile Thr Gln Met Lys Ser Lys Glu Ser Leu Ile Trp Leu Ala Ile

305 310 315 320

Asp Ala Glu Asn Leu Gly His Leu Gln Pro Gln Val Ser Ser Thr Ala

325 330 335

Leu Thr Gln Leu Lys Ser Glu Glu Pro His Ile Ser Val Ala Ala Asp

340 345 350

Ala Glu Lys Gln Glu Asp Cys Asn Ser Gln Val Phe Pro Ser Pro Ile

355 360 365

Thr Gln Met Lys Arg Glu Glu Pro Glu Ser Leu Ile Ala Thr Ser Gly

370 375 380

Glu Lys Asn Glu Lys Cys Leu Asn Glu Tyr Gln Asp Leu Ser Arg Leu

385 390 395 400

Phe Asn Asp Ala Ala Thr Pro Gln Ser Leu Leu Glu Lys Leu Arg Lys

405 410 415

Arg Leu Val Lys Glu Asp Thr Leu Asn Ile Ser Ile Gln His Gln Val

420 425 430

Gln Ile Pro Ser Phe Val Glu Cys Leu Pro Asn Ser Gly Gly Ser Thr

435 440 445

Asp Ala Gly Glu Asn Thr Arg Pro Glu Gly Lys Ala Leu Val Asn Gly

450 455 460

Val Arg Lys Thr Trp Ala Asp Met Val Glu Glu Asp Glu Arg Gln Leu

465 470 475 480

Gly Asp Val Ser Ser Thr Ile Gly Met Asp Thr Thr Lys Arg Asn Val

485 490 495

Ser Cys Lys His Ala Asn Glu Glu Met Tyr Arg Thr Pro Ser Phe Ser

500 505 510

Gln Glu Ser Ser Ala Leu Lys Arg Ser Ser Val Asp Asp His Pro Gln

515 520 525

Ser Ser Ser Ala Asp Ser Trp Arg His Ser Asp Ser Lys Ile Ser Thr

530 535 540

Asp Glu Asn Val Asn Met Lys Phe Val Arg Thr Ala Pro Gln Trp Arg

545 550 555 560

Gln Lys Lys Val Gln Asp Tyr Ser Asn Arg Val Ser Gln Arg Leu Asp

565 570 575

Thr Ser His Leu Ser Asp Arg Ala Glu Gly Thr Glu Gln Pro Pro Trp

580 585 590

Arg Ser Ser Thr Ala Gln Arg Ser Leu Phe Pro Asp Trp Lys Ser Lys

595 600 605

Cys Glu Arg Tyr Gly His Gly Tyr Val Pro Phe Cys Asp Asn Glu His

610 615 620

Phe Gln Gly Ser Ser His Phe Glu Ala Thr Asn Arg Trp Pro Lys Asn

625 630 635 640

Ala Arg Pro Trp Arg Pro Gln Asn Arg Leu Trp Val Phe Gln Glu Ile

645 650 655

Thr Asn Glu Ile Asn Gln Lys Gln Thr Arg Ala Leu Trp Leu Thr Ile

660 665 670

Tyr Thr Thr Leu Leu Phe Gly Thr Thr Ala Leu Asp Asp Thr Ala Trp

675 680 685

Phe Leu Val Glu Cys Phe His Leu Leu Ala Gly His Leu Leu Leu His

690 695 700

Asp Gly Leu Gln Asp Leu Ser Val Ala Pro Pro Ser Gly Gln Phe Ala

705 710 715 720

Glu Met Ala Met Gln Met His Pro Ala Thr Gly Leu Ser Thr His Trp

725 730 735

Ser Gln Val Asp Ser Pro Pro Leu Lys Leu Arg Glu Gly Ala Asn Met

740 745 750

Val Val Leu Leu Gln Asp Gln Met Leu Pro Met Ala Phe Ser Ile Ala

755 760 765

Asn Val Cys Glu Asp Cys Ile Met Ala Tyr Met Ser Ala Ser Ala Ser

770 775 780

Ser Val Leu Phe Tyr Thr Ser Cys Leu Asp Met Gln Gln Leu Pro Tyr

785 790 795 800

Thr Gly His Val Ile Thr Ile Asn Lys Ser Glu Thr Ala

805 810

<210> 27

<211> 685

<212> PRT

<213> 高粱（Sorghum bicolor）

<400> 27

Met Pro Ser Gly Gly Arg Arg Leu Pro Pro Trp Thr Ser Pro Arg Ser

1 5 10 15

Ala Gly Ala Gly Ala Ala Arg Trp Ser Pro Ala Ala Gly Thr Pro Ala

20 25 30

Ala Ala Gly Gly Gln Arg Ser Gly Ser Gly Tyr Gly Thr Pro Pro Leu

35 40 45

Ser Ala Gly Cys Phe Gly Thr Arg Val Thr Pro Pro Thr Ser Gly Gly

50 55 60

Ala Arg Val Thr Pro Pro Ser Thr Gly Gly Cys Ser Ser Arg Pro Pro

65 70 75 80

Arg Pro Pro Pro Ser Leu Asp Ser Pro Tyr Val Arg Ala Lys Gln Ala

85 90 95

Gln Ile Val Glu Lys Asp Pro Asn Lys Ala Val Pro Leu Phe Trp Ala

100 105 110

Ala Ile Asn Ser Gly Asp Arg Thr Glu Ser Ala Leu Lys Asp Met Ala

115 120 125

Asn Val Leu Lys Gln Ala Asn Arg Ala Glu Glu Ala Ile Glu Ala Ile

130 135 140

Arg Ser Phe Arg Asp Arg Cys Pro Tyr Glu Ala Gln Glu Ser Leu Asp

145 150 155 160

Asn Ile Leu Leu Asp Leu Tyr Lys Lys Cys Gly Arg Thr Asp Glu Gln

165 170 175

Ile Glu Met Leu Thr Ile Lys Leu Arg Ile Val Asp Glu Glu Leu Ala

180 185 190

Ser Gly Arg Trp Lys Thr Lys Met Ser Lys Ser His Gly Arg Val Val

195 200 205

Tyr Leu Ser Leu Arg Asp Glu Lys Ala Arg Leu Leu Gly Asn Leu Ala

210 215 220

Trp Ala Tyr Met Gln Ser Glu Asn Tyr Glu Gly Ala Glu Met Leu Tyr

225 230 235 240

Arg Gln Ala Leu Ala Ile Glu Ala Asp Tyr Asn Lys Glu Cys Asn Leu

245 250 255

Ala Ile Cys Leu Met Lys Thr Gly Lys Val Ala Glu Ala Lys Tyr Leu

260 265 270

Ile Gln Ala Ile Pro Tyr Asn Cys Asp Asp Glu Ser His Val Lys Ser

275 280 285

Leu Ser Arg Ala Thr Glu Met Leu Arg Glu Leu Glu Leu Gln Ser Leu

290 295 300

Pro Ser Pro Ile Thr Gln Ala Lys Ser Lys Glu Ser Gln Ile Phe Leu

305 310 315 320

Ala Asp Asp Val Glu Met Leu Val Asp Leu Gln Pro Gln Thr Leu Ser

325 330 335

Thr Pro Leu Ser Glu Leu Lys Tyr Lys Arg Pro His Ile Ser Val Ser

340 345 350

Gln Asn Ala Glu Lys His Glu Asn Cys Asn Ser Trp Leu Pro Ser Pro

355 360 365

Ile Thr Gln Leu Arg Arg Glu Glu Pro His Ile Met Val Thr Ala Gly

370 375 380

Ala Glu Lys Asn Glu Ser Phe Ala Glu Phe Gln Asp Leu Ser Arg Leu

385 390 395 400

Phe Asn Asp Ala Ala Thr Pro His Ser Ile Leu Glu Lys Leu Arg Lys

405 410 415

Arg Leu Val Lys Glu Ala Pro Lys Ile Gly Ile His Asp Asp Gln Ile

420 425 430

Gln Thr Pro Ile Pro Thr Glu Cys Leu Pro Asn Ser Glu Arg Asn Leu

435 440 445

Asp Ala Ser Glu Thr Pro Met Gln Glu Gly Lys Leu Leu Thr Lys Gly

450 455 460

Val Lys Lys Thr Trp Ala Asp Met Val Asp Glu Glu Glu Gln Gln Leu

465 470 475 480

Gly Asp Asp Lys Pro Leu Ala Asp Met Val Ala Lys Asp Glu Gln Gln

485 490 495

Leu Gly Glu Ser Lys Ser Thr Leu Gly Val Gly Thr Thr Glu Gln Lys

500 505 510

Glu Ser Ser Lys His Ala Ser Lys Leu Glu Tyr Arg Thr Pro Leu Ala

515 520 525

Ser Gln Glu Ser Arg Thr His Gln Arg Pro Phe Met Gly Gly Gln Leu

530 535 540

Gln Gly Ser Ser Ala Ala Ser Trp Arg Gln Asn Asp Ser Lys Ile Ser

545 550 555 560

Met Asp Lys Asn Val Asn Arg Asp Leu Val Arg Thr Ala Pro Thr Trp

565 570 575

Ser Lys His Lys Ala Gln Asp His Asn Asn Arg Val Trp Gln Arg Leu

580 585 590

Asp Thr Val His Pro His Glu Arg Ala Ser Asp Thr Asn Gln Val Pro

595 600 605

Arg Arg Ser Asn Thr Ser Gln Arg Ala Leu Phe Pro Asp Trp Lys Ser

610 615 620

Lys Gly Glu Gly His Gly His Gly Cys Val Leu Phe Asp Asp Asn Glu

625 630 635 640

Arg Thr Gln Cys Ser Ser His Val Glu Ala Thr His Arg Trp His Asn

645 650 655

Asn Glu Ala Ser Thr Gly Ser Trp Arg Pro Gln Asn Arg Leu Arg Val

660 665 670

Phe Gln Glu Ile Thr Asn Glu Ile Asn Gln Asn Val Val

675 680 685

<210> 28

<211> 690

<212> PRT

<213> 玉米（Zea mays）

<400> 28

Met Pro Ser Gly Gly Arg Arg Leu Pro Pro Trp Thr Ser Pro Arg Ser

1 5 10 15

Ala Gly Ala Pro Met Trp Ser Thr Ala Gly Thr Pro Gly Gly Pro Arg

20 25 30

Pro Gly Pro Gly Tyr Gly Thr Pro Pro Val Ser Ala Gly Cys Phe Gly

35 40 45

Thr Arg Val Thr Pro Pro Thr Ser Gly Gly Thr Arg Val Thr Pro Pro

50 55 60

Thr Ser Gly Gly Ala Gly Ala Arg Val Thr Pro Pro Ser Thr Gly Gly

65 70 75 80

Cys Ser Ser Arg Pro Pro Arg Pro Pro Pro Ser Met Asp Ser Pro Tyr

85 90 95

Val Arg Ala Lys Gln Ala Gln Ile Val Glu Lys Asp Pro Asn Lys Ala

100 105 110

Val Pro Leu Phe Trp Ala Ala Ile Asn Ser Gly Asp Arg Ile Glu Ser

115 120 125

Ala Leu Lys Asp Met Ala Asn Val Leu Lys Gln Ala Asn Arg Ser Glu

130 135 140

Glu Ala Ile Glu Ala Ile Arg Ser Phe Arg Asp Arg Cys Pro Tyr Glu

145 150 155 160

Ala Gln Glu Ser Leu Asp Asn Ile Leu Leu Asp Leu Tyr Lys Lys Cys

165 170 175

Gly Arg Thr Asp Glu Gln Ile Glu Met Leu Thr Leu Lys Leu Arg Ile

180 185 190

Val Asp Glu Glu Leu Ala Ser Gly Arg Trp Lys Thr Lys Leu Ser Lys

195 200 205

Ser His Gly Arg Val Val Tyr Leu Ser Leu Arg Asp Glu Lys Ala Arg

210 215 220

Leu Leu Gly Asn Leu Ala Trp Ala Tyr Met Gln Ser Glu Asn Tyr Glu

225 230 235 240

Glu Ala Glu Met Leu Tyr Arg Gln Ala Leu Ala Ile Glu Ala Asp Tyr

245 250 255

Asn Lys Glu Cys Asn Leu Ala Ile Cys Leu Met Lys Thr Gly Lys Leu

260 265 270

Ala Glu Ala Lys Tyr Leu Ile His Ala Ile Pro Tyr Asn Cys Asn Asp

275 280 285

Glu Ser His Val Lys Ser Leu Ser Arg Ala Thr Glu Met Leu Arg Glu

290 295 300

Phe Asp Leu Gln Ser Leu Pro Ser Pro Ile Thr Gln Ala Lys Ser Lys

305 310 315 320

Glu Pro Arg Asn Phe Val Ala Asp Asp Val Glu Met Leu Val Asp Leu

325 330 335

Gln Pro Gln Thr Leu Ser Thr Pro Phe Ser Glu Leu Lys Tyr Lys Glu

340 345 350

Ala His Ile Ser Val Ser Gln Asn Ala Glu Lys His Glu Asn Cys Asn

355 360 365

Ser Cys Leu Pro Ser Pro Ile Thr Gln Leu Arg Arg Glu Glu Pro His

370 375 380

Thr Met Val Thr Ala Asp Ala Glu Lys Asn Glu Gly Phe Ala Glu Phe

385 390 395 400

Gln Asp Leu Ser Arg Leu Phe Asn Asp Ala Ala Thr Pro His Ser Val

405 410 415

Leu Glu Lys Leu Arg Lys Arg Leu Val Lys Glu Ala Pro Lys Val Ser

420 425 430

Ile His Asp Gln Ile Gln Thr Pro Pro Thr Glu Ser Leu Pro Asn Ser

435 440 445

Glu Arg Asn Leu Asp Ala Ser Glu Thr Ser Val Gln Glu Gly Lys Leu

450 455 460

Leu Thr Lys Gly Val Arg Lys Thr Trp Ser Asp Met Val Asp Glu Glu

465 470 475 480

Glu Gln Gln Leu Gly Asp Asp Lys Pro Trp Ala Asp Met Val Ala Lys

485 490 495

Asp Asp Gln Gln Leu Gly Asp Gly Lys Ser Thr His Gly Val Gly Ile

500 505 510

Thr Glu Gln Asn Glu Ser Ser Lys His Ala Ser Lys Leu Glu Tyr Arg

515 520 525

Thr Pro Ser Ser Ser Gln Glu Ser Arg Thr His Gln Arg Pro Val Met

530 535 540

Gly Gly Gln Leu Gln Gly Ser Ser Ala Gly Ser Trp Arg Arg Ser Asp

545 550 555 560

Ser Lys Ile Tyr Met Asp Lys Asn Val Asn Trp Asp Leu Val Arg Thr

565 570 575

Ala Pro Thr Trp Ser Arg His Lys Val His Asp His Asn Asn Arg Val

580 585 590

Trp Gln Arg Leu His Thr Ile His Pro Arg Glu Arg Ala Ser Gly Thr

595 600 605

Lys Gln Val Pro Arg Arg Ser Asn Thr Ser Gln Arg Ala Leu Phe Pro

610 615 620

Asp Trp Lys Ser Lys Gly Glu Gly Tyr Gly His Gly Tyr Val Pro Phe

625 630 635 640

Asp Asp Asn Glu His Thr Gln Cys Ser Ser His Ile Glu Ala Ala Thr

645 650 655

His Arg Trp His Asn Asn Glu Ala Ser Thr Gly Ser Trp Arg Pro Gln

660 665 670

Asn Arg Leu Arg Val Phe Gln Glu Ile Thr Asn Glu Ile Asn Gln Asn

675 680 685

Val Val

690

<210> 29

<211> 362

<212> PRT

<213> 拟南芥（Arabidopsis thaliana）

<400> 29

Met Glu Gly Lys Phe Ala Ile Ser Glu Ser Thr Asn Leu Leu Gln Arg

1 5 10 15

Ile Lys Asp Phe Thr Gln Ser Val Val Val Asp Leu Ala Glu Gly Arg

20 25 30

Ser Pro Lys Ile Ser Ile Asn Gln Phe Arg Asn Tyr Cys Met Asn Pro

35 40 45

Glu Ala Asp Cys Leu Cys Ser Ser Asp Lys Pro Lys Gly Gln Glu Ile

50 55 60

Phe Thr Leu Lys Lys Glu Pro Gln Thr Tyr Arg Ile Asp Met Leu Leu

65 70 75 80

Arg Val Leu Leu Ile Val Gln Gln Leu Leu Gln Glu Asn Arg His Ala

85 90 95

Ser Lys Arg Asp Ile Tyr Tyr Met His Pro Ser Ala Phe Lys Ala Gln

100 105 110

Ser Ile Val Asp Arg Ala Ile Gly Asp Ile Cys Ile Leu Phe Gln Cys

115 120 125

Ser Arg Tyr Asn Leu Asn Val Val Ser Val Gly Asn Gly Leu Val Met

130 135 140

Gly Trp Leu Lys Phe Arg Glu Ala Gly Arg Lys Phe Asp Cys Leu Asn

145 150 155 160

Ser Leu Asn Thr Ala Tyr Pro Val Pro Val Leu Val Glu Glu Val Glu

165 170 175

Asp Ile Val Ser Leu Ala Glu Tyr Ile Leu Val Val Glu Lys Glu Thr

180 185 190

Val Phe Gln Arg Leu Ala Asn Asp Met Phe Cys Lys Thr Asn Arg Cys

195 200 205

Ile Val Ile Thr Gly Arg Gly Tyr Pro Asp Val Ser Thr Arg Arg Phe

210 215 220

Leu Arg Leu Leu Met Glu Lys Leu His Leu Pro Val His Cys Leu Val

225 230 235 240

Asp Cys Asp Pro Tyr Gly Phe Glu Ile Leu Ala Thr Tyr Arg Phe Gly

245 250 255

Ser Met Gln Met Ala Tyr Asp Ile Glu Ser Leu Arg Ala Pro Asp Met

260 265 270

Lys Trp Leu Gly Ala Phe Pro Ser Asp Ser Glu Val Tyr Ser Val Pro

275 280 285

Lys Gln Cys Leu Leu Pro Leu Thr Glu Glu Asp Lys Lys Arg Thr Glu

290 295 300

Ala Met Leu Leu Arg Cys Tyr Leu Lys Arg Glu Met Pro Gln Trp Arg

305 310 315 320

Leu Glu Leu Glu Thr Met Leu Lys Arg Gly Val Lys Phe Glu Ile Glu

325 330 335

Ala Leu Ser Val His Ser Leu Ser Phe Leu Ser Glu Val Tyr Ile Pro

340 345 350

Ser Lys Ile Arg Arg Glu Val Ser Ser Pro

355 360

<210> 30

<211> 383

<212> PRT

<213> 拟南芥（Arabidopsis thaliana）

<400> 30

Met Glu Glu Ser Ser Gly Leu Ser Ser Met Lys Phe Phe Ser Asp Gln

1 5 10 15

His Leu Ser Tyr Ala Asp Ile Leu Leu Pro His Glu Ala Arg Ala Arg

20 25 30

Ile Glu Val Ser Val Leu Asn Leu Leu Arg Ile Leu Asn Ser Pro Asp

35 40 45

Pro Ala Ile Ser Asp Leu Ser Leu Ile Asn Arg Lys Arg Ser Asn Ser

50 55 60

Cys Ile Asn Lys Gly Ile Leu Thr Asp Val Ser Tyr Ile Phe Leu Ser

65 70 75 80

Thr Ser Phe Thr Lys Ser Ser Leu Thr Asn Ala Lys Thr Ala Lys Ala

85 90 95

Phe Val Arg Val Trp Lys Val Met Glu Ile Cys Phe Gln Ile Leu Leu

100 105 110

Gln Glu Lys Arg Val Thr Gln Arg Glu Leu Phe Tyr Lys Leu Leu Cys

115 120 125

Asp Ser Pro Asp Tyr Phe Ser Ser Gln Ile Glu Val Asn Arg Ser Val

130 135 140

Gln Asp Val Val Ala Leu Leu Arg Cys Ser Arg Tyr Ser Leu Gly Ile

145 150 155 160

Met Ala Ser Ser Arg Gly Leu Val Ala Gly Arg Leu Phe Leu Gln Glu

165 170 175

Pro Gly Lys Glu Ala Val Asp Cys Ser Ala Cys Gly Ser Ser Gly Phe

180 185 190

Ala Ile Thr Gly Asp Leu Asn Leu Leu Asp Asn Thr Ile Met Arg Thr

195 200 205

Asp Ala Arg Tyr Ile Ile Ile Val Glu Lys His Ala Ile Phe His Arg

210 215 220

Leu Val Glu Asp Arg Val Phe Asn His Ile Pro Cys Val Phe Ile Thr

225 230 235 240

Ala Lys Gly Tyr Pro Asp Ile Ala Thr Arg Phe Phe Leu His Arg Met

245 250 255

Ser Thr Thr Phe Pro Asp Leu Pro Ile Leu Val Leu Val Asp Trp Asn

260 265 270

Pro Ala Gly Leu Ala Ile Leu Cys Thr Phe Lys Phe Gly Ser Ile Gly

275 280 285

Met Gly Leu Glu Ala Tyr Arg Tyr Ala Cys Asn Val Lys Trp Ile Gly

290 295 300

Leu Arg Gly Asp Asp Leu Asn Leu Ile Pro Glu Glu Ser Leu Val Pro

305 310 315 320

Leu Lys Pro Lys Asp Ser Gln Ile Ala Lys Ser Leu Leu Ser Ser Lys

325 330 335

Ile Leu Gln Glu Asn Tyr Ile Glu Glu Leu Ser Leu Met Val Gln Thr

340 345 350

Gly Lys Arg Ala Glu Ile Glu Ala Leu Tyr Cys His Gly Tyr Asn Tyr

355 360 365

Leu Gly Lys Tyr Ile Ala Thr Lys Ile Val Gln Gly Lys Tyr Ile

370 375 380

<210> 31

<211> 1330

<212> PRT

<213> 拟南芥（Arabidopsis thaliana）

<400> 31

Met Phe Phe Gln His Ser Gln Leu Gln Asn Ser Asp His Leu Leu His

1 5 10 15

Glu Ser Met Ala Asp Ser Asn His Gln Ser Leu Ser Pro Pro Cys Ala

20 25 30

Asn Gly His Arg Ser Thr Ile Ser Leu Arg Asp Asp Gln Gly Gly Thr

35 40 45

Phe Cys Leu Ile Cys Phe Ser Asn Leu Val Ser Asp Pro Arg Ile Pro

50 55 60

Thr Val His Val Ser Tyr Ala Leu His Gln Leu Ser Ile Ala Ile Ser

65 70 75 80

Glu Pro Ile Phe Leu Arg Thr Leu Leu Ser Ser His Ile His Phe Leu

85 90 95

Val Ser Pro Leu Val His Ala Leu Ser Ser Ile Asp Asp Ala Pro Ile

100 105 110

Ala Ile Gln Ile Met Asp Met Ile Ser Leu Leu Cys Ser Val Glu Glu

115 120 125

Ser Ser Ile Gly Glu Asp Phe Val Glu Arg Ile Ser Asp Gln Leu Ser

130 135 140

Ser Gly Ala Leu Gly Trp Ser Arg Arg Gln Leu His Met Leu His Cys

145 150 155 160

Phe Gly Val Leu Met Ser Cys Glu Asn Ile Asp Ile Asn Ser His Ile

165 170 175

Arg Asp Lys Glu Ala Leu Val Cys Gln Leu Val Glu Gly Leu Gln Leu

180 185 190

Pro Ser Glu Glu Ile Arg Gly Glu Ile Leu Phe Ala Leu Tyr Lys Phe

195 200 205

Ser Ala Leu Gln Phe Thr Glu Gln Asn Val Asp Gly Ile Glu Val Leu

210 215 220

Ser Leu Leu Cys Pro Lys Leu Leu Cys Leu Ser Leu Glu Ala Leu Ala

225 230 235 240

Lys Thr Gln Arg Asp Asp Val Arg Leu Asn Cys Val Ala Leu Leu Thr

245 250 255

Ile Leu Ala Gln Gln Gly Leu Leu Ala Asn Ser His Ser Asn Ser Ala

260 265 270

Ser Ser Met Ser Leu Asp Glu Val Asp Asp Asp Pro Met Gln Thr Ala

275 280 285

Glu Asn Val Ala Ala Arg Pro Cys Leu Asn Val Leu Phe Ala Glu Ala

290 295 300

Ile Lys Gly Pro Leu Leu Ser Thr Asp Ser Glu Val Gln Ile Lys Thr

305 310 315 320

Leu Asp Leu Ile Phe His Tyr Ile Ser Gln Glu Ser Thr Pro Ser Lys

325 330 335

Gln Ile Gln Val Met Val Glu Glu Asn Val Ala Asp Tyr Ile Phe Glu

340 345 350

Ile Leu Arg Leu Ser Glu Cys Lys Asp Gln Val Val Asn Ser Cys Leu

355 360 365

Arg Val Leu Asp Leu Phe Ser Leu Ala Glu His Ser Phe Arg Lys Arg

370 375 380

Leu Val Ile Gly Phe Pro Ser Val Ile Arg Val Leu His Tyr Val Gly

385 390 395 400

Glu Val Pro Cys His Pro Phe Gln Ile Gln Thr Leu Lys Leu Ile Ser

405 410 415

Ser Cys Ile Ser Asp Phe Pro Gly Ile Ala Ser Ser Ser Gln Val Gln

420 425 430

Glu Ile Ala Leu Val Leu Lys Lys Met Leu Glu Arg Tyr Tyr Ser Gln

435 440 445

Glu Met Gly Leu Phe Pro Asp Ala Phe Ala Ile Ile Cys Ser Val Phe

450 455 460

Val Ser Leu Met Lys Thr Pro Ser Phe Gly Glu Thr Ala Asp Val Leu

465 470 475 480

Thr Ser Leu Gln Glu Ser Leu Arg His Ser Ile Leu Ala Ser Leu Ser

485 490 495

Leu Pro Glu Lys Asp Ser Thr Gln Ile Leu His Ala Val Tyr Leu Leu

500 505 510

Asn Glu Ile Tyr Val Tyr Cys Thr Ala Ser Thr Ser Ile Asn Met Thr

515 520 525

Ser Cys Ile Glu Leu Arg His Cys Val Ile Asp Val Cys Thr Ser His

530 535 540

Leu Leu Pro Trp Phe Leu Ser Asp Val Asn Glu Val Asn Glu Glu Ala

545 550 555 560

Thr Leu Gly Ile Met Glu Thr Phe His Ser Ile Leu Leu Gln Asn Ser

565 570 575

Asp Ile Gln Ala Lys Glu Phe Ala Glu Leu Leu Val Ser Ala Asp Trp

580 585 590

Phe Ser Phe Ser Phe Gly Cys Leu Gly Asn Phe Cys Thr Asp Asn Met

595 600 605

Lys Gln Arg Ile Tyr Leu Met Leu Ser Ser Leu Val Asp Ile Leu Leu

610 615 620

Glu Gln Lys Thr Gly Ser His Ile Arg Asp Ala Leu His Cys Leu Pro

625 630 635 640

Ser Asp Pro Gln Asp Leu Leu Phe Leu Leu Gly Gln Ala Ser Ser Asn

645 650 655

Asn Gln Glu Leu Ala Ser Cys Gln Ser Ala Ala Leu Leu Ile Phe His

660 665 670

Thr Ser Ser Ile Tyr Asn Asp Arg Leu Ala Asp Asp Lys Leu Val Leu

675 680 685

Ala Ser Leu Glu Gln Tyr Ile Ile Leu Asn Lys Thr Ser Leu Ile Cys

690 695 700

Ala Ile Ser Asp Ser Pro Ala Leu Leu Asn Leu Val Asn Leu Tyr Gly

705 710 715 720

Leu Cys Arg Ser Leu Gln Asn Glu Arg Tyr Gln Ile Ser Tyr Ser Leu

725 730 735

Glu Ala Glu Arg Ile Ile Phe His Leu Leu Asn Glu Tyr Glu Trp Asp

740 745 750

Leu Gly Ser Ile Asn Ile His Leu Glu Ser Leu Lys Trp Leu Phe Gln

755 760 765

Gln Glu Ser Ile Ser Lys Ser Leu Ile Tyr Gln Ile Gln Lys Ile Ser

770 775 780

Arg Asn Asn Leu Ile Gly Asn Glu Val His Asn Val Tyr Gly Asp Gly

785 790 795 800

Arg Gln Arg Ser Leu Thr Tyr Trp Phe Ala Lys Leu Ile Ser Glu Gly

805 810 815

Asp Asn Tyr Ala Ala Thr Leu Leu Val Asn Leu Leu Thr Gln Leu Ala

820 825 830

Glu Lys Glu Glu Gln Glu Asn Asp Val Thr Ser Ile Leu Asn Leu Met

835 840 845

Asn Thr Ile Val Ser Ile Phe Pro Thr Ala Ser Asn Asn Leu Ser Met

850 855 860

Asn Gly Ile Gly Ser Val Val His Arg Leu Val Ser Gly Phe Ser Asn

865 870 875 880

Ser Ser Leu Gly Thr Ser Phe Lys Thr Leu Leu Leu Leu Val Phe Asn

885 890 895

Ile Leu Thr Ser Val Gln Pro Ala Val Leu Met Ile Asp Glu Ser Trp

900 905 910

Tyr Ala Val Ser Ile Lys Leu Leu Asn Phe Leu Ser Leu Arg Asp Thr

915 920 925

Ala Ile Lys Gln Asn His Glu Asp Met Val Val Ile Gly Ile Leu Ser

930 935 940

Leu Val Leu Tyr His Ser Ser Asp Gly Ala Leu Val Glu Ala Ser Arg

945 950 955 960

Asn Ile Val Ser Asn Ser Tyr Leu Val Ser Ala Ile Asn Thr Val Val

965 970 975

Asp Val Ala Cys Ser Lys Gly Pro Ala Leu Thr Gln Cys Gln Asp Glu

980 985 990

Thr Asn Ile Gly Glu Ala Leu Ala Phe Thr Leu Leu Leu Tyr Phe Phe

995 1000 1005

Ser Leu Arg Ser Leu Gln Ile Val Leu Ala Gly Ala Val Asp Trp

1010 1015 1020

Gln Ala Phe Phe Gly Thr Ser Thr Ser Leu Glu Thr Leu Pro Val

1025 1030 1035

Val Cys Ile Tyr Cys His Asn Leu Cys Arg Leu Met His Phe Gly

1040 1045 1050

Ala Pro Gln Ile Lys Leu Ile Ala Ser Tyr Cys Leu Leu Glu Leu

1055 1060 1065

Leu Thr Gly Leu Ser Glu Gln Val Asp Ile Lys Lys Glu Gln Leu

1070 1075 1080

Gln Cys Ser Ser Ser Tyr Leu Lys Ser Met Lys Ala Val Leu Gly

1085 1090 1095

Gly Leu Val Phe Cys Asp Asp Ile Arg Val Ala Thr Asn Ser Ala

1100 1105 1110

Leu Cys Leu Ser Met Ile Leu Gly Trp Glu Asp Met Glu Gly Arg

1115 1120 1125

Thr Glu Met Leu Lys Thr Ser Ser Trp Tyr Arg Phe Ile Ala Glu

1130 1135 1140

Glu Met Ser Val Ser Leu Ala Leu Pro Cys Ser Ala Ser Ser Thr

1145 1150 1155

Tyr Val Asn His His Lys Pro Ala Val Tyr Leu Thr Val Ala Met

1160 1165 1170

Leu Arg Leu Lys Asn Lys Pro Val Trp Leu Arg Thr Val Phe Asp

1175 1180 1185

Glu Ser Cys Ile Ser Ser Met Ile Gln Asn Leu Asn Gly Ile Asn

1190 1195 1200

Ile Ser Arg Glu Ile Val Ile Leu Phe Arg Glu Leu Met Gln Ala

1205 1210 1215

Glu Leu Leu Asn Ser Gln Gln Val Thr Lys Leu Asp Arg Ala Phe

1220 1225 1230

Gln Glu Cys Arg Lys Gln Met His Arg Asn Gly Thr Arg Asp Glu

1235 1240 1245

Thr Val Glu Glu Gln Val Gln Arg Lys Ile Pro Ser Ile His Asp

1250 1255 1260

His Ser Glu Phe Cys Asn Tyr Leu Val His Leu Met Val Ser Asn

1265 1270 1275

Ser Phe Gly His Pro Ser Glu Ser Glu Thr Tyr Thr Gln Lys Lys

1280 1285 1290

Lys Gln Ile Leu Asp Glu Met Glu Gln Leu Ser Glu Leu Ile Ser

1295 1300 1305

Thr Arg Glu Gly Arg Val Ser Pro Ile Gln Glu Glu Thr Arg Gln

1310 1315 1320

Met Gln Thr Glu Arg Ile Val

1325 1330

<210> 32

<211> 385

<212> PRT

<213> 拟南芥（Arabidopsis thaliana）

<400> 32

Met Ser Ser Ser Val Ala Glu Ala Asn His Thr Glu Lys Glu Glu Ser

1 5 10 15

Leu Arg Leu Ala Ile Ala Val Ser Leu Leu Arg Ser Lys Phe Gln Asn

20 25 30

His Gln Ser Ser Ser Ser Thr Ser Arg Cys Tyr Val Ser Ser Glu Ser

35 40 45

Asp Ala Leu Arg Trp Lys Gln Lys Ala Lys Glu Arg Lys Lys Glu Ile

50 55 60

Ile Arg Leu Gln Glu Asp Leu Lys Asp Ala Glu Ser Ser Phe His Arg

65 70 75 80

Asp Leu Phe Pro Ala Asn Ala Ser Cys Lys Cys Tyr Phe Phe Asp Asn

85 90 95

Leu Gly Val Phe Ser Gly Arg Arg Ile Gly Glu Ala Ser Glu Ser Arg

100 105 110

Phe Asn Asp Val Leu Arg Arg Arg Phe Leu Arg Leu Ala Cys Val Val

115 120 125

Ile Leu Ser Leu Ala Arg Arg Arg Ser Arg Arg Lys Leu Thr Arg Ser

130 135 140

Ser Gln Arg Leu Gln Pro Ser Glu Pro Asp Tyr Glu Glu Glu Ala Glu

145 150 155 160

His Leu Arg Ile Ser Ile Asp Phe Leu Leu Glu Leu Ser Glu Ala Asp

165 170 175

Ser Asn Asp Ser Asn Phe Ser Asn Trp Ser His Gln Ala Val Asp Phe

180 185 190

Ile Phe Ala Ser Leu Lys Lys Leu Ile Ser Met Gly Arg Asn Leu Glu

195 200 205

Ser Val Glu Glu Ser Ile Ser Phe Met Ile Thr Gln Leu Ile Thr Arg

210 215 220

Met Cys Thr Pro Val Lys Gly Asn Glu Val Lys Gln Leu Glu Thr Ser

225 230 235 240

Val Gly Phe Tyr Val Gln His Leu Ile Arg Lys Leu Gly Ser Glu Pro

245 250 255

Phe Ile Gly Gln Arg Ala Ile Phe Ala Ile Ser Gln Arg Ile Ser Ile

260 265 270

Leu Ala Glu Asn Leu Leu Phe Met Asp Pro Phe Asp Glu Ser Phe Pro

275 280 285

Glu Met Asp Glu Cys Met Phe Ile Leu Ile Gln Leu Ile Glu Phe Leu

290 295 300

Ile Cys Asp Tyr Leu Leu Pro Trp Ala Asn Glu Ala Phe Asp Asn Val

305 310 315 320

Met Phe Glu Glu Trp Ile Ala Ser Val Val His Ala Arg Lys Ala Val

325 330 335

Lys Ala Leu Glu Glu Arg Asn Gly Leu Tyr Leu Leu Tyr Met Asp Arg

340 345 350

Val Thr Gly Glu Leu Ala Lys Arg Val Gly Gln Ile Thr Ser Phe Arg

355 360 365

Glu Val Glu Pro Ala Ile Leu Asp Lys Ile Leu Ala Tyr Gln Glu Ile

370 375 380

Glu

385

210> 33

<211> 449

<212> PRT

<213> 拟南芥（Arabidopsis thaliana）

<400> 33

Met Lys Met Asn Ile Asn Lys Ala Cys Asp Leu Lys Ser Ile Ser Val

1 5 10 15

Phe Pro Pro Asn Leu Arg Arg Ser Ala Glu Pro Gln Ala Ser Gln Gln

20 25 30

Leu Arg Ser Gln Gln Ser Gln Gln Ser Phe Ser Gln Gly Pro Ser Ser

35 40 45

Ser Gln Arg Gly Cys Gly Gly Phe Ser Gln Met Thr Gln Ser Ser Ile

50 55 60

Asp Glu Leu Leu Ile Asn Asp Gln Arg Phe Ser Ser Gln Glu Arg Asp

65 70 75 80

Leu Ser Leu Lys Lys Val Ser Ser Cys Leu Pro Pro Ile Asn His Lys

85 90 95

Arg Glu Asp Ser Gln Leu Val Ala Ser Arg Ser Ser Ser Gly Leu Ser

100 105 110

Arg Arg Trp Ser Ser Ala Ser Ile Gly Glu Ser Lys Ser Gln Ile Ser

115 120 125

Glu Glu Leu Glu Gln Arg Phe Gly Met Met Glu Thr Ser Leu Ser Arg

130 135 140

Phe Gly Met Met Leu Asp Ser Ile Gln Ser Asp Ile Met Gln Ala Asn

145 150 155 160

Arg Gly Thr Lys Glu Val Phe Leu Glu Thr Glu Arg Ile Gln Gln Lys

165 170 175

Leu Thr Leu Gln Asp Thr Ser Leu Gln Gln Leu Arg Lys Glu Gln Ala

180 185 190

Asp Ser Lys Ala Ser Leu Asp Gly Gly Val Lys Phe Ile Leu Glu Glu

195 200 205

Phe Ser Lys Asp Pro Asn Gln Glu Lys Leu Gln Lys Ile Leu Gln Met

210 215 220

Leu Thr Thr Ile Pro Glu Gln Val Glu Thr Ala Leu Gln Lys Ile Gln

225 230 235 240

Arg Glu Ile Cys His Thr Phe Thr Arg Glu Ile Gln Val Leu Ala Ser

245 250 255

Leu Arg Thr Pro Glu Pro Arg Val Arg Val Pro Thr Ala Pro Gln Val

260 265 270

Lys Ala Lys Glu Asn Leu Pro Glu Gln Arg Gly Gln Ala Ala Lys Val

275 280 285

Leu Thr Ser Leu Lys Met Pro Glu Pro Arg Val Gln Val Pro Ala Ala

290 295 300

Pro Gln Ala Lys Glu Asn Phe Pro Glu Gln Arg Gly Pro Val Ala Lys

305 310 315 320

Ser Asn Ser Phe Cys Asn Thr Thr Leu Lys Thr Lys Gln Pro Gln Phe

325 330 335

Pro Arg Asn Pro Asn Asp Ala Ser Ala Arg Ala Val Lys Pro Tyr Leu

340 345 350

Ser Pro Lys Ile Gln Val Gly Cys Trp Lys Thr Val Lys Pro Glu Lys

355 360 365

Ser Asn Phe Lys Lys Arg Ala Thr Arg Lys Pro Val Lys Ser Glu Ser

370 375 380

Thr Arg Thr Gln Phe Glu Gln Cys Ser Val Val Ile Asp Ser Asp Glu

385 390 395 400

Glu Asp Ile Asp Gly Gly Phe Ser Cys Leu Ile Asn Glu Asn Thr Arg

405 410 415

Gly Thr Asn Phe Glu Trp Asp Ala Glu Lys Glu Thr Glu Arg Ile Leu

420 425 430

Arg Thr Ala Arg Arg Thr Lys Arg Lys Phe Gly Asn Pro Ile Ile Ile

435 440 445

Asn

<210> 34

<211> 233

<212> PRT

<213> 拟南芥（Arabidopsis thaliana）

<400> 34

Met Arg His Asn Ile Lys Phe Lys Ser Lys Gly Thr Leu Lys Ile Arg

1 5 10 15

Asn Thr Ala Gln Ile Ser Leu Trp Lys Lys Cys Ser Asp Ser Met Ile

20 25 30

Ala Asp Gln Thr Tyr Leu Phe Ile Asn Arg Val Gln Asp Arg Arg Phe

35 40 45

Asp Glu Glu Ser Leu Arg Ile Leu Glu Leu Ser Leu Val Ala Met Asn

50 55 60

Val Lys Ser Phe Leu Glu Val Arg Ser Arg Leu Arg Asp Phe Met Arg

65 70 75 80

Ser Glu Ser Val Val Ile Phe Gly Glu Leu Thr Gly Glu Ser Met Val

85 90 95

Ala Lys Leu Ser Val Leu Glu Phe Phe Ala Arg Ala Phe Ala Leu Leu

100 105 110

Gly Asp Met Glu Ser Cys Leu Ala Met Arg Tyr Glu Ala Leu Asn Leu

115 120 125

Arg Gln Leu Lys Ser Pro Ser Cys Leu Trp Leu Gly Val Ser His Ser

130 135 140

Glu Trp Thr Lys Phe Ala Val Gln Ser Met Glu Asn Gly Phe Pro Ser

145 150 155 160

Ile Ala Gly Lys Ala Ser Glu Asn Ala Leu Leu Ser Leu Lys Lys Asp

165 170 175

Ser Leu Ile Glu Pro Lys Ser Glu Asp Asn Ser Asp Ile Leu Asp Ala

180 185 190

Ala Glu Lys Val Arg Arg Leu Arg Asp Ser Ala Ala Ser Leu Thr Ser

195 200 205

Ser His Ser Gly Ile Phe Ile Tyr Ile Val Ser Ser Leu Lys Phe Ala

210 215 220

Val Cys Asn Arg Leu Leu Thr Thr Phe

225 230

<210> 35

<211> 617

<212> PRT

<213> 拟南芥（Arabidopsis thaliana）

<400> 35

Met Phe Tyr Ser His Gln Leu Leu Ala Arg Lys Ala Pro Leu Gly Gln

1 5 10 15

Ile Trp Met Ala Ala Thr Leu His Ala Lys Ile Asn Arg Lys Lys Leu

20 25 30

Asp Lys Leu Asp Ile Ile Gln Ile Cys Glu Glu Ile Leu Asn Pro Ser

35 40 45

Val Pro Met Ala Leu Arg Leu Ser Gly Ile Leu Met Gly Gly Val Val

50 55 60

Ile Val Tyr Glu Arg Lys Val Lys Leu Leu Phe Asp Asp Val Asn Arg

65 70 75 80

Phe Leu Val Glu Ile Asn Gly Ala Trp Arg Thr Lys Ser Val Pro Asp

85 90 95

Pro Thr Leu Leu Pro Lys Gly Lys Thr His Ala Arg Lys Glu Ala Val

100 105 110

Thr Leu Pro Glu Asn Glu Glu Ala Asp Phe Gly Asp Phe Glu Gln Thr

115 120 125

Arg Asn Val Pro Lys Phe Gly Asn Tyr Met Asp Phe Gln Gln Thr Phe

130 135 140

Ile Ser Met Arg Leu Asp Glu Ser His Val Asn Asn Asn Pro Glu Pro

145 150 155 160

Glu Asp Leu Gly Gln Gln Phe His Gln Ala Asp Ala Glu Asn Ile Thr

165 170 175

Leu Phe Glu Tyr His Gly Ser Phe Gln Thr Asn Asn Glu Thr Tyr Asp

180 185 190

Arg Phe Glu Arg Phe Asp Ile Glu Gly Asp Asp Glu Thr Gln Met Asn

195 200 205

Ser Asn Pro Arg Glu Gly Ala Glu Ile Pro Thr Thr Leu Ile Pro Ser

210 215 220

Pro Pro Arg His His Asp Ile Pro Glu Gly Val Asn Pro Thr Ser Pro

225 230 235 240

Gln Arg Gln Glu Gln Gln Glu Asn Arg Arg Asp Gly Phe Ala Glu Gln

245 250 255

Met Glu Glu Gln Asn Ile Pro Asp Lys Glu Glu His Asp Arg Pro Gln

260 265 270

Pro Ala Lys Lys Arg Ala Arg Lys Thr Ala Thr Ser Ala Met Asp Tyr

275 280 285

Glu Gln Thr Ile Ile Ala Gly His Val Tyr Gln Ser Trp Leu Gln Asp

290 295 300

Thr Ser Asp Ile Leu Cys Arg Gly Glu Lys Arg Lys Val Arg Gly Thr

305 310 315 320

Ile Arg Pro Asp Met Glu Ser Phe Lys Arg Ala Asn Met Pro Pro Thr

325 330 335

Gln Leu Phe Glu Lys Asp Ser Ser Tyr Pro Pro Gln Leu Tyr Gln Leu

340 345 350

Trp Ser Lys Asn Thr Gln Val Leu Gln Thr Ser Ser Ser Glu Ser Arg

355 360 365

His Pro Asp Leu Arg Ala Glu Gln Ser Pro Gly Phe Val Gln Glu Arg

370 375 380

Met His Asn His His Gln Thr Asp His His Glu Arg Ser Asp Thr Ser

385 390 395 400

Ser Gln Asn Leu Asp Ser Pro Ala Glu Ile Leu Arg Thr Val Arg Thr

405 410 415

Gly Lys Gly Ala Ser Val Glu Ser Met Met Ala Gly Ser Arg Ala Ser

420 425 430

Pro Glu Thr Ile Asn Arg Gln Ala Ala Asp Ile Asn Val Thr Pro Phe

435 440 445

Tyr Ser Gly Asp Asp Val Arg Ser Met Pro Ser Thr Pro Ser Ala Arg

450 455 460

Gly Ala Ala Ser Ile Asn Asn Ile Glu Ile Ser Ser Lys Ser Arg Met

465 470 475 480

Pro Asn Arg Lys Arg Pro Asn Ser Ser Pro Arg Arg Gly Leu Glu Pro

485 490 495

Val Ala Glu Glu Arg Pro Trp Glu His Arg Glu Tyr Glu Phe Glu Phe

500 505 510

Ser Met Leu Pro Glu Lys Arg Phe Thr Ala Asp Lys Glu Ile Leu Phe

515 520 525

Glu Thr Ala Ser Thr Gln Thr Gln Lys Pro Val Cys Asn Gln Ser Asp

530 535 540

Glu Met Ile Thr Asp Ser Ile Lys Ser His Leu Lys Thr His Phe Glu

545 550 555 560

Thr Pro Gly Ala Pro Gln Val Glu Ser Leu Asn Lys Leu Ala Val Gly

565 570 575

Met Asp Arg Asn Ala Ala Ala Lys Leu Phe Phe Gln Ser Cys Val Leu

580 585 590

Ala Thr Arg Gly Val Ile Lys Val Asn Gln Ala Glu Pro Tyr Gly Asp

595 600 605

Ile Leu Ile Ala Arg Gly Pro Asn Met

610 615

<210> 36

<211> 21

<212> DNA

<213> 人工序列

<220>

<223> 合成引物

<400> 36

gacatcggca cttgcttaga g 21

<210> 37

<211> 21

<212> DNA

<213> 人工序列

<220>

<223> 合成引物

<400> 37

gcgatatagc tcccactggt t 21

<210> 38

<211> 31

<212> DNA

<213> 人工序列

<220>

<223> 合成引物

<400> 38

gagtttacta tactctgccg ccggcgagaa c 31

<210> 39

<211> 38

<212> DNA

<213> 人工序列

<220>

<223> 合成引物

<400> 39

ctccacctgg agtttactat gccccgccgc cggcgaga 38

<210> 40

<211> 32

<212> DNA

<213> 人工序列

<220>

<223> 合成引物

<400> 40

ccacctggag ttgcgtatac tccgccgcgg cg 32

<210> 41

<211> 35

<212> DNA

<213> 人工序列

<220>

<223> 合成引物

<400> 41

ccacctggag ttgcgagaac aagtgatcat gtggc 35