经遗传修饰的表达海藻糖酶的酵母及使用此类经遗传修饰的酵母的发酵方法与流程
相关申请的交叉引用
本申请要求于2017年5月4日提交的美国临时专利申请号62/501,288、2018年2月28日提交的美国临时专利申请号62/636,716和2018年3月27日提交的美国临时专利申请号62/648,679的权益，所有所述美国临时专利申请由此通过引用整体并入。
序列表
2018年5月4日创建的大小为761千字节的标题为“n00525_st25.txt”的ascii文本文件的全部内容由此通过引用并入本文。
发明背景
海藻糖是在包括酿酒酵母在内的微生物中产生的二糖。海藻糖通常是由于生物体受到应激而产生的。海藻糖酶是催化海藻糖向葡萄糖转化的糖苷水解酶。一些微生物可以天然产生中性ph海藻糖酶和/或酸性海藻糖酶。然而，野生型酵母不会产生大量的海藻糖酶。
技术实现要素：
本发明涉及可以表达异源海藻糖酶的遗传工程改造的酵母。由酵母表达的海藻糖酶通过将由酵母产生的海藻糖或以其它方式存在于发酵液中的海藻糖转化为葡萄糖而增加了来自发酵的乙醇产量。
一方面，本公开涉及包含编码海藻糖酶(ec3.2.1.28)的异源基因的经遗传修饰的酵母，其中当酵母存在于包含海藻糖的发酵培养基中时，该酵母能够产生乙醇。在一些实施方案中，海藻糖酶是酸性海藻糖酶。在一些实施方案中，海藻糖酶是中性海藻糖酶。在一些实施方案中，酵母编码酸性海藻糖酶和中性海藻糖酶。在一些实施方案中，编码海藻糖酶的基因来自乳酸克鲁维酵母(kluyveromyceslactis)。在一些实施方案中，编码海藻糖酶的基因来自近平滑假丝酵母(candidaparapsilosis)。在一些实施方案中，编码海藻糖酶的基因来自光滑假丝酵母(candidaglabrata)。在一些实施方案中，编码海藻糖酶的基因来自稻瘟病菌(magnaporthegrisea)。在一些实施方案中，由酵母编码的海藻糖酶多肽与以下多肽序列中的至少一个具有至少75％、80％、85％、90％、95％或97％的序列同一性：seqidno：1、seqidno：2、seqidno：3或seqidno：87。在一些实施方案中，由酵母编码的海藻糖酶多肽包括与seqidno：83具有至少70％、80％、90％或95％的序列同一性的序列。在一些实施方案中，由酵母编码的海藻糖酶多肽包括与seqidno：84和/或seqidno：85具有至少70％、至少75％、至少80％、至少85％、至少90％、至少94％或100％的序列同一性的序列。在一些实施方案中，酵母是经遗传修饰的酿酒酵母。在一些实施方案中，由酵母编码的海藻糖酶多肽与以下多肽序列中的至少一个具有至少75％、80％、85％、90％、95％或97％的序列同一性：seqidno：88、seqidno：89、seqidno：90或seqidno：91。
在一些实施方案中，酵母包含对于海藻糖酶所源自的物种是非天然的异源海藻糖酶的信号序列。在一些实施方案中，异源海藻糖酶包含非天然信号序列。在一些实施方案中，异源海藻糖酶包含其天然信号序列。在一些实施方案中，信号序列是mfα2信号序列。在一些实施方案中，mfα2信号序列是seqidno：4。在一些实施方案中，mfα2信号序列与seqidno：4具有至少84％、89％或94％的序列同一性。在一些实施方案中，由酵母编码的海藻糖酶多肽与以下多肽序列中的至少一个具有至少75％、80％、85％、90％、95％或97％的序列同一性：seqidno：8、seqidno：11、seqidno：14或seqidno：92。
一方面，酵母包含至少一种编码除海藻糖酶外的多肽的异源基因。在一些实施方案中，酵母包含编码葡糖淀粉酶(ec3.2.1.3)的异源基因。在一些实施方案中，编码葡糖淀粉酶的异源基因是来自选自由以下组成的组的物种的葡糖淀粉酶基因：树脂枝胞霉(cladosporiumresinae)、黑曲霉(aspergillusniger)、泡盛曲霉(aspergillusawamori)、米曲霉(aspergillusoryzae)、白曲霉(aspergilluskawachii)、白宇佐美曲霉(aspergillusshirousami)、食腺嘌呤芽生葡萄孢酵母(blastobotrysadeninivorans)、白假丝酵母(candidaalbicans)、米根霉(rhizopusoryzae)、粟酒裂殖酵母(schizosaccharomycespombe)、扣囊复膜酵母菌(saccharomycopsisfibuligera)、布鲁塞尔酒香酵母(brettanomycesbruxellensis)和产朊假丝酵母(cyberlindnerajadinii)。在一些实施方案中，由酵母编码的葡糖淀粉酶与以下多肽序列中的至少一个具有至少70％、80％、90％或95％的序列同一性：seqidno：16、seqidno：17、seqidno：18或seqidno：19。在一些实施方案中，酵母包含编码异麦芽糖酶(ec3.2.1.10)的异源基因。在一些实施方案中，酵母包含与seqidno：20的多肽具有至少70％、80％、90％或95％的序列同一性的糖转运蛋白的异源基因。在一些实施方案中，酵母包含与seqidno：21的多肽具有至少70％、80％、90％或95％的序列同一性的糖转运蛋白的异源基因。
一方面，酵母包括与乳酸消耗有关的特征。在一些实施方案中，酵母包含编码细胞色素b2(cyb2)(ec1.1.2.3)多肽的异源基因。在一些实施方案中，cyb2多肽具有氨基酸序列，所述氨基酸序列与以下氨基酸序列中的任一个具有至少50％、55％、65％、70％、75％、80％、85％、90％、95％、97％或99％的序列同一性：seqidno:27、seqidno:28、seqidno:29、seqidno:30、seqidno:31、seqidno:32或seqidno:33。在一些实施方案中，酵母编码cyb2多肽，所述cyb2多肽在seqidno:27、seqidno:28、seqidno:29、seqidno:30、seqidno:31、seqidno:32或seqidno:33中的指示位置处包含一个或多个以下残基：lys349、tyr143、tyr254和his373。在一些实施方案中，酵母包含编码d乳酸脱氢酶(dld)(ec1.1.2.4)多肽的异源基因。在一些实施方案中，dld多肽具有氨基酸序列，所述氨基酸序列与以下氨基酸序列中的任一个具有至少50％、55％、65％、70％、75％、80％、85％、90％、95％、97％或99％的序列同一性：seqidno:34、seqidno:35、seqidno:36、seqidno:37、seqidno:38、seqidno:39、seqidno:40或seqidno:41。在一些实施方案中，酵母包含编码单羧酸/单羧酸盐转运蛋白的异源基因。在一些实施方案中，由酵母编码的单羧酸/单羧酸盐转运蛋白具有氨基酸序列，所述氨基酸序列与以下氨基酸序列中的任一个具有至少50％、55％、65％、70％、75％、80％、85％、90％、95％、97％或99％的序列同一性：seqidno:22、seqidno:23、seqidno:24、seqidno:25或seqidno:26。
在一些实施方案中，酵母以足以在乙醇滴度为至少为75g/l时，将发酵液中的海藻糖含量降低至小于0.5g/l、1g/l、2g/l、3g/l、4g/l、5g/l、6g/l、7g/l、8g/l、9g/l或10g/l的量分泌海藻糖酶。在一些实施方案中，酵母能够在细胞外分泌海藻糖酶。在一些实施方案中，由酵母编码的海藻糖酶多肽与seqidno：86具有至少76％、至少84％、至少92％或100％的序列同一性。在一些实施方案中，酵母能够以80g/l、90g/l、100g/l、110g/l、120g/l、125g/l、130g/l、135g/l或更高的滴度产生乙醇。
一方面，本公开涉及使用本文所述的任何酵母的方法。在一个实施方案中，该方法是用于生产乙醇的方法，其包括：使用经遗传修饰的酵母发酵培养基，其中所述酵母包含异源海藻糖酶基因，其中发酵结束时的乙醇滴度为至少90g/l。在一些实施方案中，该方法的发酵温度在25至45℃、25至40℃、25至35℃、30至40℃或28至38℃的范围内。在一些实施方案中，发酵结束时的乙醇滴度为至少80、90、100、110、120、130、135、140、145、150、155或160g/升。
一方面，本公开涉及经遗传修饰的酵母，其包含编码与seqidno：1(乳酸克鲁维酵母)具有至少80％的序列同一性的海藻糖酶(ec3.2.1.28)多肽的异源基因，其中当酵母存在于包含海藻糖的发酵培养基中时，酵母能够产生乙醇，并且当乙醇滴度为至少110g/l时，酵母以足以将发酵液中的海藻糖含量降低至小于2g/l的量分泌海藻糖酶。一方面，本公开涉及经遗传修饰的酵母，其包含编码与seqidno：2(近平滑假丝酵母)具有至少80％的序列同一性的海藻糖酶(ec3.2.1.28)多肽的异源基因，其中当酵母存在于包含海藻糖的发酵培养基中时，酵母能够产生乙醇，并且当乙醇滴度为至少110g/l时，酵母以足以将发酵液中的海藻糖含量降低至小于2g/l的量分泌海藻糖酶。一方面，本公开涉及经遗传修饰的酵母，其包含编码与seqidno：3(光滑假丝酵母)具有至少80％的序列同一性的海藻糖酶(ec3.2.1.28)多肽的异源基因，其中当酵母存在于包含海藻糖的发酵培养基中时，酵母能够产生乙醇，并且当乙醇滴度为至少110g/l时，酵母以足以将发酵液中的海藻糖含量降低至小于2g/l的量分泌海藻糖酶。一方面，本公开涉及经遗传修饰的酵母，其包含编码与seqidno：87(稻瘟病菌)具有至少80％的序列同一性的海藻糖酶(ec3.2.1.28)多肽的异源基因，其中当酵母存在于包含海藻糖的发酵培养基中时，酵母能够产生乙醇，并且当乙醇滴度为至少110g/l时，酵母以足以将发酵液中的海藻糖含量降低至小于2g/l的量分泌海藻糖酶。
在一些实施方案中，由酵母编码的海藻糖酶多肽包含与seqidno：83具有至少70％、80％、90％或95％的序列同一性的序列。在一些实施方案中，由酵母编码的海藻糖酶多肽包含与seqidno：84和/或seqidno：85具有至少70％、至少75％、至少80％、至少85％、至少90％、至少94％或100％的序列同一性的序列。在一些实施方案中，酵母是经遗传修饰的酿酒酵母。在一些实施方案中，由酵母编码的海藻糖酶包含mfα2信号序列。在一些实施方案中，mfα2信号序列是seqidno：4。在一些实施方案中，mfα2信号序列与seqidno：4具有至少84％、89％或94％的序列同一性。在一些实施方案中，由酵母编码的海藻糖酶与以下多肽序列中的至少一个具有至少75％、80％、85％、90％、95％或97％的序列同一性：seqidno：8、seqidno：11、seqidno：14或seqidno：92。
在一个方面，酵母还包含编码葡糖淀粉酶(ec3.2.1.3)多肽的异源基因。在一些实施方案中，由酵母编码的葡糖淀粉酶与以下多肽序列中的至少一个具有至少70％、75％、80％、85％、90％或95％的序列同一性：seqidno:16(sfga)、seqidno:17(roga)、seqidno:108(rmicga)或seqidno:109(rdelga)。
在一个方面，酵母以足以在乙醇滴度为至少为110g/l时，将发酵液中的海藻糖含量降低至小于0.5g/l或1g/l的量分泌海藻糖酶。在一些实施方案中，酵母能够在细胞外分泌海藻糖酶。在一些实施方案中，由酵母编码的海藻糖酶多肽包含与seqidno：86具有至少76％、至少84％、至少92％或100％的序列同一性的序列。
在一个方面，酵母包含编码甘油醛-3-磷酸脱氢酶(e.c.1.2.1.9)的重组核酸；以及减少或消除的编码甘油-3-磷酸磷酸酶(ec3.1.3.21)的基因的表达。在一个方面，酵母包含编码甘油醛-3-磷酸脱氢酶(gapn，e.c.1.2.1.9)的重组核酸。在一些实施方案中，编码甘油醛-3-磷酸脱氢酶(e.c.1.2.1.9)的重组核酸编码与seqidno：111(蜡样芽孢杆菌(bacilluscereus)gapn)具有至少80％、85％、90％或95％的序列同一性的多肽。
在一些实施方案中，酵母能够以80g/l、90g/l、100g/l、110g/l、120g/l、125g/l、130g/l、135g/l或更高的滴度产生乙醇。在一些实施方案中，所述酵母与不表达异源海藻糖酶的酵母相比，产生更高的乙醇滴度和/或收率。在一些实施方案中，所述酵母与不表达异源海藻糖酶但在其它方面与所述酵母相同的酵母相比，产生更高的乙醇滴度和/或收率。在一些实施方案中，所述酵母与表达不同的异源海藻糖酶的酵母相比，产生更高的乙醇滴度和/或产量。
在一个方面，本公开涉及用于生产乙醇的方法，其包括：使用经遗传修饰的酵母发酵培养基，其中所述酵母包含编码与以下多肽序列中的至少一个或多个具有至少80％的序列同一性的海藻糖酶(ec3.2.1.28)多肽的异源海藻糖酶基因：seqidno：1(乳酸克鲁维酵母)、seqidno：2(近平滑假丝酵母)、seqidno：3(光滑假丝酵母)或seqidno：87(稻瘟病菌)，其中如在接种后36小时所测得的，发酵结束时的乙醇滴度为至少为105g/l，并且发酵结束时发酵液的海藻糖含量小于2g/l。在一些实施方案中，所述方法的酵母包含编码与以下多肽序列中的仅一个具有至少80％的序列同一性的海藻糖酶(ec3.2.1.28)多肽的异源海藻糖酶基因：seqidno：1(乳酸克鲁维酵母)、seqidno：2(近平滑假丝酵母)、seqidno：3(光滑假丝酵母)或seqidno：87(稻瘟病菌)。应当理解，用于生产乙醇的方法中使用的酵母可以是本文所述的酵母的任何实施方案，包括与任何其它所述的一种或多种性状或一种或多种修饰组合描述的任何性状或修饰。
在一些实施方案中，发酵温度的范围为25至45℃、25至40℃、25至35℃、30至40℃或28至38℃。在一些实施方案中，发酵结束时的乙醇滴度为至少120g/升、130g/升、135g/升、140g/升、145g/升、150g/升、155g/升或160g/升。在一些实施方案中，酵母是本文描述的任何实施方案或方面的酵母。
发酵液中乙醇和海藻糖含量的值可以根据以下实施例1中所述的摇瓶法进行评估和测量。
还应理解，如本领域技术人员将理解的，上述过程、方法或组合物的任何实施方案的要素或方面可应用于任何其他实施方案。
附图说明
当结合附图一起阅读时，将更好地理解本发明的以下详细说明。然而，应当理解，本发明并不限于在附图中所示的实施方案的精确安排和手段。
图1是显示使用不同的酵母菌株的发酵中的海藻糖浓度随时间变化的图。
图2和图3是显示不添加海藻糖的发酵中的乙醇量的图。
图4是显示添加海藻糖的发酵中海藻糖浓度随时间变化的图。
图5和图6是显示添加海藻糖的发酵中的乙醇量的图。
图7是显示添加海藻糖的发酵中海藻糖浓度随时间变化的图。
图8和图9是显示添加海藻糖的发酵中的乙醇量的图。
图10是显示使用不同酵母菌株的玉米醪发酵中的乙醇量的图。
图11是显示使用不同酵母菌株的玉米醪发酵中的海藻糖量的图。
具体实施方式
应当理解，本文提供的本发明的附图和描述已经简化，以举例说明与清楚理解本发明相关的要素，同时消除了在一个或多个相关领域中发现的其它要素。本领域普通技术人员将认识到，在实施本发明时可能期望或需要其他要素或步骤。然而，因为此类要素和步骤是本领域熟知的，或者不利于更好地理解本发明，本文不提供对此类要素和步骤的讨论。
定义
除非另外定义，否则本文所用的所有技术和科学术语都具有与本发明所属领域中的技术人员通常所理解相同的含义。如本文中所用，以下术语中的每一个均具有与其在本章节中相关的含义。
发酵方法定义
如本文中所用，“接种”被定义为将能够产生发酵产物的微生物引入发酵培养基中时的时间点。这是本领域的技术人员熟知的术语。
如本文中所用，“发酵结束”被定义为发酵方法满足预定标准时的时间点。预定标准可包括以下中的任一项：预定时间间隔、所供应的期望分数的碳源的耗尽、碳源消耗的停止或发酵产物形成的停止。在一个实施方案中，“发酵结束”被定义为开始收获生物产物时的时间点。如本领域技术人员将理解的，“发酵结束”可以指根据发酵方法的规模和目的而不同的时间点。对于大规模生产发酵方法，“发酵结束”优选是开始收获生物产物时的时间点，即在产物形成已有效停止之后。
如本文中所用，“细胞干重”是指如在发酵样品中所测量的测量时存在于发酵培养基中的干细胞质量的浓度。细胞干重通常以克/升(g/l)为单位表示。
如本文中所用，“接种时的细胞干重”是指如在发酵样品中所测量的接种后即刻的存在于发酵培养基中的干细胞质量的浓度。对于分批补料发酵，基于发酵培养基的最终体积计算初始细胞干重。干细胞重量的测量是本领域技术人员已知的方法。接种时的细胞干重通常以g/l为单位表示。
如本文中所用，“发酵结束时的细胞干重”是指如在发酵样品中所测量的发酵结束时存在于发酵培养基中的干细胞质量的浓度。发酵结束时的细胞干重通常以g/l为单位表示。
如本文中所用，“最终滴度”是指发酵结束时发酵液中物质的浓度。最终滴定度通常以g/l为单位表示。
如本文中所用，“初始滴度”是指接种时存在的物质的浓度。初始滴定度通常以g/l为单位表示。
如本文中所用，“批次时间”是指在接种和发酵结束之间经过的时间量。批次时间通常以小时(h)为单位表示。
如本文中所用，用于分批过程的“发酵生产速率”是指发酵产物的最终滴度减去初始滴度(最终滴度减去初始滴度)再除以批次时间。生产速率通常以克/升-小时(gl-1h-1)为单位表示。当应用于连续或半连续过程时，使用本领域已知的方法测定“发酵生产速率”。
如本文中所用，“比生产速率”是指发酵生产速率除以发酵结束时的细胞干重。比生产速率通常以(g产物)(g细胞)-1h-1为单位表示。当应用于连续或半连续过程时，使用本领域已知的方法测定“比生产速率”。
如本文中所用，发酵产物的“产物收率”是指两个量的比率：a)发酵方法中产生的产物(例如，琥珀酸酯)的质量(分子)，b)添加至发酵中的碳源的质量(分母)。产物收率通常以克/克(g/g)乘以100为单位表示。应特别注意的是，产物收率是以质量比计算的。产生的发酵产物的质量应考虑到批次结束时发酵培养基中存在的发酵产物的质量，以及批次过程中收获的任何发酵产物的质量，减去批次开始时存在的发酵产物的质量，以及进一步减去在批次过程中添加的任何发酵产物的质量。除了在批次过程中添加的任何一种或多种碳源的质量外，添加到批次中的碳源的质量还应包括批次开始时发酵罐中存在的所有碳源的质量。
如本文中所用，“耗氧速率”(“our”)是指发酵期间消耗氧气的体积速率。入口和出口的氧气浓度可通过废气分析，例如通过质谱仪来测量。可由相关领域的普通技术人员使用在bioreactionengineeringprinciples第2版，2003,kluweracademic/plenumpublishers，第449页，方程式1中描述的直接方法来计算our。其通常以(mmolo2)l-1h-1为单位来测量。
如本文中所用，“比耗氧速率”是指发酵期间消耗氧气的比速率。其计算为our与测得的细胞干重之比。其通常以mmolo2(g细胞干重)-1h-1为单位来测量。
酵母特征定义
术语“经遗传修饰的”和“经遗传工程改造的”在本文可互换使用，并且是指生物体的遗传物质的任何改变，或指如此改变的生物体。对于本公开的目的，这些术语不意味着受改变方法的限制。
在某些实施方案中，本文提供的经遗传修饰的酵母细胞还包含一种或多种天然基因的缺失或破坏。如本文中所用，关于天然基因的短语“缺失或破坏”是指基因的整个编码区被消除(缺失)或基因的编码区、其启动子和/或其终止子区被修饰(诸如通过缺失、插入或突变)，从而使得基因不再产生活性酶，产生大大减少的量(至少75％的减少，优选至少90％的减少)的活性酶，或产生活性大大降低(降低至少75％，优选降低至少90％)的酶。
在某些实施方案中，一种或多种天然基因的缺失或破坏导致一种或多种天然代谢途径的缺失或破坏。关于代谢途径的短语“缺失或破坏”是指该途径是无效的或另外地表现出相对于天然途径降低了至少75％、至少85％或至少95％的活性。
在一些实施方案中，天然基因的缺失或破坏可以通过强制进化、诱变或遗传工程改造方法，随后进行适当的选择或筛选以鉴定所需的突变体来完成。在一些实施方案中，天然宿主细胞基因的缺失或破坏可与一种或多种外源基因至宿主细胞中的整合偶联，即，可使用也是缺失构建体的基因表达整合构建体整合外源基因。在一些实施方案中，可使用不含外源基因的缺失构建体或通过本领域已知的其它方法来完成缺失或破坏。
如本文中所用，关于遗传组分的术语“异源的”是指遗传组分存在于微生物的修饰形式中，但不存在于特定微生物细胞的天然形式的基因组中。在一些实施方案中，异源遗传组分可以是细胞天然组分的修饰形式，其可源自另一生物，其可以是源自另一生物的组分的修饰形式，或者其可以是合成来源的组分。在优选的实施方案中，将异源遗传组分整合到经修饰的微生物的基因组中。例如，乳酸克鲁维酵母海藻糖酶基因在引入酿酒酵母中时是异源的。
如本文中所用，术语“外源的”是指源自目标微生物之外的任何材料。例如，术语“外源的”可应用于在遗传修饰之前不以特定生物的天然形式存在的遗传物质(即，这种外源遗传物质也可以称为异源的)，或者其也可被应用于并非源自特定生物体的酶或其它蛋白质。
检查两种核酸或两种多肽的核酸或氨基酸序列将揭示所比较的序列之间的序列同一性和相似性。序列比对和序列同一性的产生包括通常使用计算方法进行的全局比对和局部比对。可使用blast(国家生物信息中心(ncbi)基本局部比对搜索工具)2.2.31版软件，利用默认参数进行比对。氨基酸序列之间的氨基酸序列同一性％可使用利用以下默认参数的标准蛋白质blast来测定：最大靶序列：100；短查询：自动调整短输入序列的参数；预期阈值：10；字长：6；查询范围内的最大匹配数：0；矩阵：blosum62；空位成本(gapcost)：(存在：11，延伸：1)；组成调整：条件式组成得分矩阵调整；过滤器：没有选择；掩码：没有选择。核酸序列之间的核酸序列同一性％可使用利用以下默认参数的标准核苷酸blast来测定：最大靶序列：100；短查询：自动调整短输入序列的参数；预期阈值：10；字长：28；查询范围内的最大匹配数：0；匹配/不匹配分数：1，-2；空位成本：线性；过滤器：低复杂度区域；掩码：仅用于查找表的掩码。使用利用默认参数的ncbiblast2.2.31版算法相对于参考序列具有xx％(例如，80％)的同一性分数的序列被认为与参考序列至少xx％相同或等同、与参考序列具有xx％的序列同一性。
贯穿本公开，本发明的各个方面可以以范围的形式呈现。应当了解，以围形式呈现的描述仅仅是为了方便和简洁，并且不应理解为对本发明范围的硬性限制。因此，范围的描述应被认为具有确切公开的所有可能的子范围以及该范围内的单独数值。例如，对诸如1至7的范围的描述应被认为具体公开了诸如1至3、1至4、1至6、2至5、3至5等的子范围，以及该范围内的各个数字，例如1、2、3、3.6、4、5、5.8、6、7和其间的任何整数及分数增量。不管范围有多宽，这都适用。
描述
本文描述了可用于生产发酵产物的经遗传修饰的酵母菌株和使用这些酵母的发酵方法。酵母菌株经修饰以包括一种或多种异源海藻糖酶基因。在一些实施方案中，酵母菌株还可包括其它异源基因，例如异源葡糖淀粉酶基因，而对发酵方法中所需的异源酶表达水平和/或酵母性能没有任何显著不利影响。
遗传工程改造的酵母
海藻糖酶是催化海藻糖向葡萄糖转化的糖苷水解酶。在一个方面，本文所述的遗传工程改造的(ge)酵母已被修饰为包括异源海藻糖酶基因。在一些实施方案中，ge酵母由酿酒酵母宿主酵母细胞产生。在一些实施方案中，宿主酵母细胞是适合于乙醇生产的酵母菌株，例如ethanolredtm或酿酒酵母的类似菌株。因此，在一些实施方案中，ge酵母耐受乙醇发酵方法中使用的条件，诸如相对高的温度和/或乙醇浓度。在一些实施方案中，与宿主细胞相比，包含异源海藻糖酶基因可改善热和/或乙醇耐受性。
在一些实施方案中，ge酵母可包含用于表达来自以下物种中的一种或多种的海藻糖酶多肽的一种或多种基因：乳酸克鲁维酵母、近平滑假丝酵母和光滑假丝酵母。在一些实施方案中，ge酵母表达与以下氨基酸序列中的至少一个具有至少70％、75％、80％、85％、90％、95％或97％的序列同一性的海藻糖酶多肽：seqidno：1(乳酸克鲁维酵母)、seqidno：2(近平滑假丝酵母)或seqidno：3(光滑假丝酵母)。在一些实施方案中，ge酵母表达来自稻瘟病菌的海藻糖酶多肽(seqidno：87)。在一些实施方案中，ge酵母表达与seqidno:87具有至少70％、75％、80％、85％、90％、95％或97％的序列同一性的海藻糖酶多肽。在一个方面，ge酵母可包括过表达的天然海藻糖酶基因而非异源海藻糖酶基因，或者除了异源海藻糖酶基因以外还包括过表达的天然海藻糖酶基因，例如，源自酿酒酵母宿主酵母的ge酵母中的过表达的酿酒酵母海藻糖酶基因。对于本公开和权利要求的目的，任何过表达的天然基因和由这样的基因编码的任何多肽都将被认为是异源的。
在一些实施方案中，ge酵母可包含用于表达来自以下物种中的一个或多个物种的海藻糖酶多肽的一种或多种基因，其中每个物种的相关多肽序列都包含在物种名称后的括号中：酿酒酵母(seqidno：42)、德尔布有孢圆酵母(torulasporadelbrueckii)(seqidno：43)、西氏哈萨克酵母(kazachstanianaganishii)(seqidno：44)、蟑螂四盾酵母(tetrapisisporablattae)(seqidno：45)、鲁氏酵母(zygosaccharomycesrouxii)(seqidno：46)、副贝氏丝酵母(zygosaccharomycesparabailii)(seqidno：47)、毕赤四盾酵母(tetrapisisporaphaffii)(seqidno：48)、棉假囊酵母(eremotheciumgossypii)(seqidno：49)、鼻窦假囊酵母(eremotheciumsinecaudum)(seqidno：50)、米拉氏拉茜斯酵母(lachanceamirantina)(seqidno：51)、近平滑假丝酵母(candidaorthopsilosis)(seqidno：52)、麦芽糖假丝酵母(candidamaltose)(seqidno：53)、热带假丝酵母(candidatropicalis)(seqidno：54)、白假丝酵母(seqidno：55)、长孢洛德酵母(lodderomyceselongisporus)(seqidno：56)、杜氏假丝酵母(candidadubliniensis)(seqidno：57)、木糖发酵酵母(spathasporapassalidarum)(seqidno：58)、树干毕赤酵母(scheffersomycesstipitis)(seqidno：59)、法布里德巴利酵母(debaryomycesfabryi)(seqidno：60)、坦扎瓦丝假丝酵母(candidatanzawaensis)(seqidno：61)、多布克鲁维酵母(kluyveromycesdobzhanskii)(seqidno：62)、马克斯克鲁维酵母(kluyveromycesmarxianus)(seqidno：63)、鲁氏接合酵母(zygosaccharomycesrouxii)(seqidno：64)、达氏诺莫维酵母(naumovozymadairenensis)(seqidno：65)、耐热拉茜斯酵母(lachanceathermotolerans)(seqidno：66)、魁北克拉茜斯酵母(lachanceaquebecensis)(seqidno：67)、毕赤四盾酵母(seqidno：68)、发酵拉茜斯酵母(lachanceafermentati)(seqidno：69)、假山毛榉拉茜斯酵母(lachanceanothofagi)(seqidno：70)、蟑螂四盾酵母(seqidno：71)、小麦顶囊壳(gaeumannomycestritici)(seqidno：72)、夏季斑枯病菌(magnaporthiopsispoae)(seqidno：73)、嗜热毁丝霉(thermothelomycesthermophila)(seqidno：74)、若虫炭疽菌(colletotrichumnymphaeae)(seqidno：75)、兰花炭疽菌(colletotrichumorchidophilum)(seqidno：76)、木生锥毛壳(coniochaetaligniaria)(seqidno：77)、土生梭抱壳霉(thielaviaterrestris)(seqidno：78)、足马杜拉分枝菌(madurellamycetomatis)(seqidno：79)、粗糙脉孢菌(neurosporacrassa)(seqidno：80)、大丽轮枝菌(verticilliumdahlia)(seqidno：81)和/或玉蜀黍赤霉(gibberellazeae)(seqidno：82)。ge酵母可表达与这些氨基酸序列中的任何一个或多个氨基酸序列具有至少70％、75％、80％、85％、90％、95％或97％的序列同一性的海藻糖酶多肽。
在一些实施方案中，ge酵母编码来自一种物种的异源海藻糖酶多肽，所述物种包括特征模式或基序，即，与来自另一物种的海藻糖酶的氨基酸亚组相同或几乎相同的海藻糖酶多肽的全序列中的氨基酸亚组。例如，来自seqidno：1(乳酸克鲁维酵母)、seqidno：2(近平滑假丝酵母)以及seqidno：3(光滑假丝酵母)的海藻糖酶的每一种包含以下氨基酸基序：qpyvangyigsripn(seqidno:83)。在一些实施方案中，ge酵母表达与seqidno：83具有至少70％、至少80％、至少85％、至少90％或100％的序列同一性的海藻糖酶多肽。另外，来自seqidno：1(乳酸克鲁维酵母)、seqidno：2(近平滑假丝酵母)以及seqidno：3的海藻糖酶的每一种以大于90％的序列同一性包含两个以下基序：gvaglssdsyggmvfwd(seqidno:84)和nitleysgmnssveikqadv(seqidno:85)。在一些实施方案中，ge酵母表达具有与seqidno:84和/或seqidno:85具有至少70％、至少75％、至少80％、至少85％、至少90％、至少94％或100％的序列同一性的序列的一部分的海藻糖酶多肽。以下氨基酸序列包含在大多数或所有酸性海藻糖酶中：nitleysgmnssv(seqidno:86)。在一些实施方案中，ge酵母表达具有与seqidno：86具有至少76％、至少84％、至少92％或100％的序列同一性的序列的一部分的海藻糖酶多肽。已知酿酒酵母具有表达两种类型的海藻糖酶(酸性海藻糖酶(at)和中性海藻糖酶(nt))的天然海藻糖酶基因，所述海藻糖酶根据最佳的表达ph来表征。然而，两种天然海藻糖酶均受到严格调节并且显示出低活性。在低应激或无应激条件下，几乎不产生这些海藻糖酶。另外，这些海藻糖酶可被限制在液泡中。(关于酿酒酵母海藻糖酶的论述，参见，例如，parrou,j.l.,jules,m.,beltran,g.,
zilli等描述了光滑假丝酵母海藻糖酶在酵母属中的异源表达(zilli,d.m.w.,lopes,r.g.,alves,s.l.,barros,l.m.,miletti,l.c.,stambuk,b.u.secretionoftheacidtrehalaseencodedbythecgath1geneallowstrehalosefermentationbycandidaglabrata(2015)microbiologicalresearch,179，第12-19页)。然而，包括光滑假丝酵母海藻糖酶的zilli中的酵母菌株产生极低量的乙醇，即，不适用于商业过程的乙醇的量。本发明的ge酵母可以产生显著更高的商业上可用的乙醇量。
在一个方面，本发明的ge酵母表达海藻糖酶，其在细胞外大量分泌，而不是如在天然酿酒酵母的海藻糖酶中看到的那样在液泡中表达并束缚在其中。因此，表达的海藻糖酶可作用于发酵液中的细胞外海藻糖，即在发酵方法中海藻糖作为酵母的代谢物产生。在一些实施方案中，ge酵母比野生型酵母分泌更多的海藻糖酶，但是不产生对酵母造成显著的代谢负担的量的海藻糖酶，即，酵母产生足够的海藻糖酶来消耗细胞产生的大部分或全部海藻糖而不会引起负面影响发酵性能的其它问题。在一些实施方案中，酵母可包括启动子，所述启动子与防止酵母产生对细胞造成代谢负担或以其它方式负面影响发酵性能的量的海藻糖酶相关联。
对于本公开的目的，在一个方面，海藻糖酶是来自ec3.2.1.28的酶。因此，海藻糖酶是其主要活性为水解海藻糖的任何酶。在一个方面，海藻糖酶是在合理的时间范围(timeframe)内对海藻糖表现出显著活性的任何酶。
在一个方面，可通过包含肽信号序列(即前导序列)来优化或改善海藻糖酶在ge酵母中的表达，所述前导序列不同于与某些海藻糖酶相关联的野生型前导序列。在一些实施方案中，信号肽是mfα2信号序列。在一个方面，本公开的表达的海藻糖酶蛋白可包含与seqidno：4具有约79％或更高、84％或更高、89％或更高或94％或更高序列同一性的信号序列，所述信号序列源自酿酒酵母交配因子α2基因(scmfα2)的n端。在一些实施方案中，scmfα2ss序列如下：mkfistfltfilaavsvta(seqidno:4)。scmfα2序列来自基因ygl089c(ygl089c)，而mfα1由基因ypl187wmfα1编码，并且mfα2是mata细胞分泌的信息素。scmfα2-分泌信号修饰的海藻糖酶多肽及表达其的工程改造的酵母菌株描述于2016年2月5日提交的国际专利申请序列号pct/us2016/016822(miller，等)中。酿酒酵母交配因子α2(scmfα2)分泌信号描述于美国专利第4,546,082号(kurjan等)中。在一些实施方案中，酵母包含用于表达由酵母编码的海藻糖酶的基因，所述海藻糖酶与以下多肽序列中的至少一个具有至少75％、80％、85％、90％、95％或97％的序列同一性：seqidno：8、seqidno：11、seqidno：14或seqidno：92(即，具有scmfα2而非其天然分泌信号的海藻糖酶)。
在一些实施方案中，mfα2分泌信号是与seqidno：8的多肽序列具有至少80％、85％、90％或95％的序列同一性的乳酸克鲁维酵母酸性海藻糖酶分泌信号。在一些实施方案中，mfα2分泌信号是与seqidno：11的多肽序列具有至少80％、85％、90％或95％的序列同一性的近平滑假丝酵母酸性海藻糖酶分泌信号。在一些实施方案中，mfα2分泌信号是与seqidno：14的多肽序列具有至少80％、85％、90％或95％的序列同一性的光滑假丝酵母酸性海藻糖酶分泌信号。
本公开并不意味着限于任何特定的海藻糖酶多肽，并且ge酵母可包括表达可用于发酵方法的任何海藻糖酶多肽的基因。在一些实施方案中，将酸性海藻糖酶基因整合到ge酵母中。在一些实施方案中，将中性海藻糖酶基因整合到ge酵母中。在一些实施方案中，ge酵母可包含酸性海藻糖酶基因和中性海藻糖酶基因。如本领域技术人员将理解的，可以根据发酵液的预期ph、发酵方法的其它特征和/或ge酵母本身的其它特征选择启动子或前导序列来优化酸性海藻糖酶和/或中性海藻糖酶的表达。
另外，本公开并不意味着限于整合到ge酵母中的一种或多种海藻糖酶基因的任何特定的前导序列。本文所述的任何分泌信号序列可与本文所述的任何海藻糖酶多肽一起使用，即，本公开意在包括前导序列和海藻糖酶多肽的每种组合。因此，ge酵母可包括与ge酵母本身异源的海藻糖酶的前导序列。这样的前导序列可以是与异源海藻糖酶相关联的野生型，或者对于ge酵母和从中获取海藻糖酶的物种都可以是异源的。在一些实施方案中，ge酵母中不同基因的天然前导序列可以与异源海藻糖酶一起使用。
对于本公开的目的，当鉴定序列与任何海藻糖酶多肽的序列同一性百分比时，应理解天然前导序列氨基酸不包括在序列同一性的计算中。然而，如果前导序列氨基酸不能使用此类方法来容易且完全确定，则使用全长海藻糖酶多肽序列(即包括天然前导序列)来计算序列同一性百分比。例如，seqidno：87是具有其天然前导序列的稻瘟病菌海藻糖酶的多肽，而seqidno：88是没有其天然前导序列的稻瘟病菌海藻糖酶的多肽。没有分泌前导序列的海藻糖酶多肽的其它实例包括seqidno：89(光滑假丝酵母)、seqidno：90(近平滑假丝酵母)和seqidno：91(乳酸克鲁维酵母)。因此，在一些实施方案中，ge酵母编码与seqidno:88、seqidno:89、seqidno:90或seqidno:91具有至少75％、至少80％、至少90％、至少95％、至少97％或100％的序列同一性的异源海藻糖酶多肽。在一些实施方案中，ge酵母编码异源海藻糖酶多肽，所述异源海藻糖酶多肽与以下代表具有mfα2分泌信号的海藻糖酶多肽的序列中的任一个具有至少75％、至少80％、至少90％、至少95％、至少97％或100％的序列同一性：seqidno：4与seqidno：88的组合、seqidno：4与seqidno：89的组合、seqidno：4与seqidno：90的组合或seqidno：4与seqidno：91的组合。
尽管不希望受到理论束缚，但序列分析软件仍预测乳酸克鲁维酵母、近平滑假丝酵母和光滑假丝酵母海藻糖酶全部三种的天然前导序列(信号肽)是跨膜结构域，而不是分泌信号。这表明海藻糖酶被从细胞膜推出到周质空间(即细胞膜和细胞壁之间的空间)中。然而，这些海藻糖酶可能仍锚定在细胞膜上。用mfα2分泌信号替代天然信号肽可能会将所述蛋白质从膜上解脱出来，这使得能够实现细胞外分泌。
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在一个方面，可通过包含启动子来优化或改善海藻糖酶在ge酵母中的表达。在一些实施方案中，启动子是tdh3启动子。在一个实施方案中，启动子是与seqidno：5具有至少80％、85％、90％或95％的序列同一性的酿酒酵母tdh3启动子。在一些实施方案中，启动子是sam2启动子。在一些实施方案中，启动子是与seqidno：112具有至少80％、85％、90％或95％的序列同一性的酿酒酵母sam2启动子。
在一个方面，ge酵母表达海藻糖酶解决了通常与用于通过发酵生产生物产物的ge酵母相关的重大问题。酵母在受到应激时通常会产生更多的海藻糖。一些发酵方法条件可在用于生物产物生产的ge酵母中造成应激。例如，一些发酵方法与高温相关，这导致对酵母的应激。高乙醇或其它生物产物浓度和/或高盐浓度也会造成增加海藻糖产生的应激。ge酵母中异源酶的表达还可导致海藻糖产生的增加，因为这种酶的表达可造成对酵母的应激。特别地，已知表达葡糖淀粉酶的工程改造的酵母表现出更高的海藻糖产生。
然而，本发明的表达海藻糖酶的酵母可以通过减少或消除由用于生物产物发酵的酵母产生的海藻糖来解决该问题。海藻糖被转化为葡萄糖，并且可以被酵母用作用于代谢需要和/或生物产物形成的碳源。因此，被酵母用于产生海藻糖的碳可以被酵母有效地再循环以制备蛋白质或生物产物，从而改善了使用酵母的发酵方法的总体性能。
在一些研究中，与本公开的教导相反，已在酵母中缺失或破坏天然海藻糖酶基因以试图减少应激问题并增加乙醇耐受性(参见，例如，trevisol,e.t.v.,panek,a.d.,mannarino,s.c.,eleutherio,e.c.a.,theeffectoftrehaloseonthefermentationperformanceofagedcellsofsaccharomycescerevisiae(2011)appliedmicrobiologyandbiotechnology,90(2)，第697-704页，其论述了酸性海藻糖酶(ath1)或中性海藻糖酶(nth1)的缺失以及由此增强的乙醇耐受性)。然而，令人惊奇地显示，与野生型或其它工程改造的酵母相比，分泌异源海藻糖酶的本发明的ge酵母可改善乙醇生产性能。
在一些实施方案中，ge酵母还可包含用于表达除海藻糖酶外的多肽的异源基因。在一些实施方案中，ge酵母可包括用于表达以下的任一种或全部的一个或多个异源基因：淀粉酶，例如葡糖淀粉酶(ec3.2.1.3)、与乳酸消耗相关的蛋白质(例如，编码单羧酸/单羧酸盐转运蛋白的异源基因和/或一个或多个编码乳酸脱氢酶(细胞色素)(分类为ec1.1.2.3或1.1.2.4)的异源基因))、异麦芽糖酶(ec3.2.1.10)和糖转运蛋白。在一些实施方案中，ge酵母还可包含用于优化此类多肽的表达的一种或多种启动子和/或前导序列。在一些实施方案中，ge酵母可包含2个或更多个拷贝的本文所述的任何异源基因。
葡糖淀粉酶(e.c.3.2.1.3)是淀粉分解酶，其从寡糖链和多糖链的非还原性末端相继水解1,4-连接的a-d-葡糖基残基并释放d-葡萄糖。在一些实施方案中，ge酵母编码异源海藻糖酶和异源葡糖淀粉酶两者。
在以下参考文献中进一步描述了上述遗传修饰，所述参考文献的全部内容由此通过引用整体并入：2016年2月5日提交的wo2016/127083(modifiedglucoamylaseenzymesandyeaststrainshavingenhancedethanolproduction)；2016年3月25日提交的wo2016/160584(glucoamylase-modifiedyeaststrainsandmethodsforbioproductproduction)；2016年12月16日提交的pct/us16/067314(公布为wo2017/106739)(sugartransporter-modifiedyeaststrainsandmethodsforbioproductproduction)；2016年8月5日提交的美国专利申请62/371,681(公布为wo2018/027131)(leader-modifiedglucoamylasepolypeptidesandengineredyeaststrainhavingenhancedbioproductproduction)；2016年9月16日提交的美国专利申请62/395,792(公布为wo2018/053230)(geneticallymodifiedlactate-consumingyeastsandfermentationprocessesusingsuchgeneticallymodifiedyeasts)。
在一些实施方案中，ge酵母可包含具有以下seqid和/或表达以下多肽seqid中的任一个或多个的基因，其包括以上参考文献中描述的遗传修饰的实施方案：与seqidno:16(扣囊复膜孢酵母)葡糖淀粉酶(ga)多肽的氨基酸19-515)、seqidno:17(与米根霉ga多肽的氨基酸26-604)、seqidno:18(白宇佐美曲霉ga多肽的氨基酸19-639)和/或seqidno:19(土曲霉(aspergillusterreus)ga多肽的氨基酸21-636)具有大于50％、60％、70％、75％、80％、85％、90％或95％的序列同一性；与seqidno:108(小孢根霉(rhizopusmicrosporus)ga多肽)具有大于75％、80％、81％、85％、90％或95％或更高的序列同一性；与seqidno:109(戴尔根霉(rhizopusdelemar)ga多肽)具有大于80％、85％、90％、95％或97％或更高的序列同一性；与seqidno:20(米卡塔酵母(saccharomycesmikatae)糖转运多肽)具有大于80％、85％、90％或95％或更高的序列同一性；与seqidno:21(酿酒酵母mal11)具有大于50％、60％、70％、80％、85％、90％或95％或更高的序列同一性；与以下氨基酸序列中的至少一个具有至少65％、70％、75％、80％、85％、90％、95％、97％或99％的序列同一性的氨基酸序列：seqidno:22、seqidno:23、seqidno:24、seqidno:25,或seqidno:26(异源单羧酸盐/质子同向转运蛋白氨基酸，例如jen1同向转运蛋白，来自东方伊萨酵母(issatchenkiaorientalis)、酿酒酵母、乳酸克鲁维酵母、多布克鲁维酵母或马克斯克鲁维酵母)；具有与以下氨基酸序列中的至少一个具有至少50％、55％、60％、65％、70％、75％、80％、85％、90％、95％、97％或99％的序列同一性的氨基酸序列的多肽：seqidno:27、seqidno:28、seqidno:29、seqidno:30、seqidno:31、seqidno:32或seqidno:33(来自酿酒酵母、东方伊萨酵母、克鲁弗酵母(saccharomyceskluyveri)、贝酵母(saccharomycesbayanus)、鲁氏接合酵母(zygosaccharomycesrouxii)、乳酸克鲁维酵母或多布克鲁维酵母的细胞色素b2(cyb2)多肽)；或与以下氨基酸序列中的至少一个具有至少50％、55％、60％、65％、70％、75％、80％、85％、90％、95％、97％或99％的序列同一性的氨基酸序列：seqidno:34、seqidno:35、seqidno:36、seqidno:37、seqidno:38、seqidno:39、seqidno:40或seqidno:41(来自酿酒酵母、东方伊萨酵母、克鲁弗酵母、贝酵母、烟曲霉、乳酸克鲁维酵母、多布克鲁维酵母或马克斯克鲁维酵母的d-乳酸脱氢酶(dld)多肽)。在一个方面，lys349、tyr143、tyr254和his373的残基和相关位置在seqidno:27、seqidno:28、seqidno:29、seqidno:30、seqidno:31、seqidno:32或seqidno:33中是保守的。由ge酵母编码的任何上述seqid可以是如上文中定义的异源的。
如本领域技术人员将理解的，修饰宿主酵母以包含多个异源基因可导致对宿主酵母的不可预测的作用。例如，含有多个异源基因(即2个或更多个表达不同类别的酶或其它蛋白质的异源基因)的ge酵母可对酵母产生负面的代谢作用，不利地影响酵母的耐热性，或不利地影响酵母产生的乙醇量。然而，令人惊讶地发现，在本文所述的至少一些实施方案中，包含异源海藻糖酶基因对ge酵母没有任何明显的不利作用。相反，与不包含所有多个性状的酵母相比，包含多个性状(即，多个不同的异源基因)的ge酵母在乙醇发酵方面的性能更好。
在一个方面，已经发现，与未修饰的宿主细胞相比，在酵母中包含一种或多种表达异源酶的基因，例如表达葡糖淀粉酶的基因可导致酵母的海藻糖产量增加。另外，还发现与突变前的宿主细胞相比，通过诱变进行遗传修饰以改善耐热性、乙醇耐受性或其它特征的酵母显示出增加的海藻糖产量。然而，与未修饰的宿主细胞或除包含整合的异源海藻糖基因外具有所有相同修饰的宿主细胞相比，本发明的ge酵母可显示出较低的海藻糖产量。在一些实施方案中，即使当将本发明的ge酵母与不含异源海藻糖酶的酵母进行比较时海藻糖的产量是相似的，表达海藻糖酶的ge酵母也可通过能够将大多数或全部产生的海藻糖转化成葡萄糖(所述葡萄糖可被进一步转化成乙醇和/或被酵母用于代谢)来产生更多乙醇。
另外，在一个方面，ge酵母可减少或消除向发酵中添加某些外源酶的需要，这些外源酶即如由本文所述的酵母表达任何酶，诸如海藻糖酶和葡糖淀粉酶，从而引起显著的成本节省。
在一个方面，由ge酵母表达海藻糖酶可引起在发酵期间更高的乙醇产量。在一些实施方案中，ge酵母可比不表达异源海藻糖酶的酵母产生多至少0.1％、0.2％、0.3％、0.4％、0.5％、0.6％、0.7％、0.8％、0.9％、1.0％、1.1％、1.2％、1.3％、1.4％或1.5％的乙醇。在一些实施方案中，表达异源海藻糖酶的ge酵母可以以至少70g/l、75g/l、80g/l、85g/l、90g/l、95g/l、100g/l、105g/l、110g/l、115g/l、120g/l、125g/l、130g/l或135g/l或更大的滴度产生乙醇。在一些实施方案中，酵母是表达海藻糖酶的ge酵母，并且可用于使纤维素或半纤维素培养基发酵。在此类实施方案中，乙醇滴度可以低于其它乙醇生产过程中的滴度，并且仍然是商业上可行的过程。例如，这种表达海藻糖酶的酵母可以产生至少40g/l、45g/l、50g/l、55g/l、60g/l或65g/l的乙醇滴度。
在一个方面，ge酵母表达足够量的海藻糖酶，以在发酵结束时将至少25％、33％、50％、60％、70％、80％或90％的产生的或以其它方式存在于发酵液中的海藻糖转化为葡萄糖。在一个方面，ge酵母表达足够量的海藻糖酶以在发酵结束时将发酵液中的海藻糖的量减少至小于0.5g/l、1g/l、1.5g/l、2g/l、2.5g/l、3g/l、3.5g/l、4g/l、4.5g/l、5g/l、6g/l、7g/l、8g/l、9g/l或10g/l。在一些实施方案中，ge酵母表达足够量的海藻糖酶以将发酵液中的海藻糖的量减少至小于0.5g/l、1g/l、1.5g/l、2g/l、2.5g/l、3g/l、3.5g/l、4g/l、4.5g/l、5g/l、6g/l、7g/l、8g/l、9g/l或10g/l而无需向发酵中添加外源海藻糖酶，也就是说，将所述海藻糖的量降至此类水平所必需的所有海藻糖酶由酵母菌分泌。在一些实施方案中，当乙醇滴定度为至少40g/l、45g/l、50g/l、55g/l、60g/l、65g/l、70g/l、75g/l、80g/l、85g/l、90g/l、95g/l、100g/l、105g/l、110g/l、115g/l、120g/l、125g/l、130g/l或135g/l时，ge酵母可将海藻糖酶的量减少至前述水平中的任何水平。在一个方面，ge酵母表达足够量的海藻糖酶，以在发酵方法结束前转化总共至少1g/l、2g/l、3g/l、4g/l、5g/l、6g/l、7g/l、8g/l、9g/l、10g/l或更多的在发酵方法中产生和/或存在的海藻糖。尽管设想了由ge酵母产生的大多数海藻糖酶被分泌到细胞外以作用于酵母细胞外的海藻糖，即存在于发酵液中的海藻糖，但也设想了由ge酵母产生的一部分海藻糖酶可保留在细胞中，其可在所述细胞中作用于细胞内的海藻糖。在一个方面，ge酵母可包含与减少量的副产物(包括甘油)相关的遗传修饰。这些遗传修饰(或遗传修饰的组合)在本文中可以称为“甘油还原性状”。在一个方面，ge酵母包含以下修饰：编码甘油醛-3-磷酸脱氢酶(e.c.1.2.1.9)的重组核酸，以及减少或消除的编码甘油-3-磷酸磷酸酶(e.c.3.1.3.21)的基因的表达。在美国申请号62/648,679(其由此通过引用整体并入)中进一步描述了这些修饰。在一个方面，ge酵母包含以下修饰：编码甘油醛-3-磷酸脱氢酶(e.c.1.2.1.9)的重组核酸。
本文所述的工程改造的酵母菌株可包含参与甘油生产的一种或多种酶的遗传修饰。例如，本文描述的工程改造的酵母菌株可具有减少或消除的一种或多种编码甘油-3-磷酸磷酸酶(gpp；对应于e.c.3.1.3.21；也称为“甘油-1-磷酸酶”)的基因的表达。甘油-3-磷酸磷酸酶将甘油-3-磷酸水解为甘油，从而调节甘油-3-磷酸(葡萄糖、脂质和能量代谢的代谢中间产物)的细胞水平(mugabo等，pnas(2016)113:e430-439)。
酿酒酵母(酿酒酵母)具有两种甘油-3-磷酸磷酸酶旁系同源物，分别称为gpp1p和gpp2p，分别由gpp1(uniprot号p41277)和gpp2(uniprot号p40106)基因编码(norbeck等(1996)j.biol.chem.10271(23):13875-81；pahlman等(2001)j.biol.chem.276(5):3555-63)。在一些实施方案中，ge酵母具有减少或消除的gpp1的表达。在其它实施方案中，ge酵母具有减少或消除的gpp2的表达。在其它实施方案中，ge酵母具有减少或消除的gpp1和gpp2两者的表达。
应当理解，实现减少或消除的编码甘油-3-磷酸磷酸酶的基因的表达的任何手段都与本发明的方面相容。例如，可以通过破坏基因和/或一个或多个调控区(控制该基因表达)的序列，诸如通过将一个或多个突变或插入引入到基因的序列中或一个或多控制该基因表达的调控区中，来实现减少或消除的编码甘油-3-磷酸磷酸酶的基因的表达。
在一些实施方案中，编码甘油-3-磷酸磷酸酶的基因，例如gpp1基因的表达减少至少约10％、20％、30％、40％、50％、60％、70％、80％、90％或100％。在一些实施方案中，编码甘油-3-磷酸磷酸酶的基因，诸如gpp1基因的表达被消除。在一些实施方案中，编码甘油-3-磷酸磷酸酶的基因，诸如gpp1基因的表达可通过本领域普通技术人员已知的任何手段，例如通过将核酸片段插入到gpp1基因座或或围绕gpp1基因座的调控区中来消除。
在一些实施方案中，ge酵母是二倍体并且具有减少或消除的两个拷贝的gpp1基因的表达。在一些实施方案中，ge酵母是二倍体并且在两个拷贝的gpp1基因中都包含缺失和/或插入。
本文所述的工程改造的酵母可具有减少或消除的一种或多种编码甘油醛-3-磷酸脱氢酶(gpd；对应于e.c.1.2.1.12)的基因的表达。酿酒酵母具有两种甘油醛-3-磷酸脱氢酶，称为gpd1p和gpd2p，分别由gpd1(uniprot号q00055)和gpd2(uniprot号p41911)基因编码。在一些实施方案中，ge酵母具有减少或消除的gpd1的表达。在其它实施方案中，ge酵母具有减少或消除的gpd2的表达。在其它实施方案中，ge酵母具有减少或消除的gpd1和表达gpd2两者的表达。
应当理解，实现减少或消除的编码甘油醛-3-磷酸脱氢酶的基因的表达的任何手段都与本发明的方面相容。例如，减少或消除的编码甘油醛-3-磷酸脱氢酶的基因的表达可通过破坏基因的序列和/或控制该基因表达的一个或多个调控区，例如通过将一个或多个突变或插入引入到基因序列中或控制所述基因表达的一个或多个调控区中来实现。
在一些实施方案中，编码甘油醛-3-磷酸脱氢酶的基因，诸如gpd1基因的表达减少至少约10％、20％、30％、40％、50％、60％、70％、80％、90％或100％。在一些实施方案中，编码甘油醛-3-磷酸脱氢酶的基因，诸如gpd1基因的表达被消除。编码甘油醛-3-磷酸脱氢酶的基因，诸如gpd1基因的表达可通过本领域普通技术人员已知的任何手段，诸如通过将核酸片段插入到gpd1基因座中或围绕gpd1基因座的调控区中来消除。
在一些实施方案中，本文所述的ge酵母，诸如酿酒酵母是二倍体并且具有减少或消除的两个拷贝的gpd1基因的表达。在一些实施方案中，ge酵母是二倍体并且在两个拷贝的gpd1基因中都包含缺失和/或插入。在其它实施方案中，ge酵母具有减少或消除的一个拷贝的gpd1基因的表达。
在一些实施方案中，本文所述的工程改造的酵母，诸如酿酒酵母，具有减少或消除的gpp1和/或gpp2的表达，并且还具有减少或消除的gpd1和/或gpd2的表达。在某些实施方案中，本文所述的工程改造的酵母诸如酿酒酵母，具有减少或消除的两个拷贝的gpp1的表达并且还具有减少或消除的一个拷贝的gpd1的表达。
本文所述的工程改造的酵母重组地表达一种或多种编码甘油醛-3-磷酸脱氢酶(gapn；对应于e.c.1.2.1.9；也称为“nadp依赖性非磷酸化甘油醛-3-磷酸脱氢酶”)的核酸。gapn酶将d-甘油醛3-磷酸转化为3-磷酸-d-甘油酯(rosenberg等，biolchem(1955)217:361-71)。
应当理解，编码gapn酶的重组核酸可以来自任何来源。重组表达编码gapn酶的核酸的工程改造的酵母可包含或可以不包含编码gapn酶的内源基因。
在一些实施方案中，重组表达编码gapn酶的核酸的工程改造的酵母不包含编码gapn酶的基因的内源拷贝。因此，在此类实施方案中，编码gapn酶的核酸源自不同于所述工程改造的酵母的物种或生物体。
在其它实施方案中，重组表达编码gapn酶的核酸的工程改造的酵母确实包含编码gapn酶的基因的内源拷贝。在一些此类实施方案中，编码gapn酶的基因的内源拷贝或该基因的调控区，诸如启动子，被工程改造以增加编码gapn酶的基因的表达。在其它此类实施方案中，将编码gapn酶的核酸引入到酵母中。在此类实施方案中，被引入到酵母中的编码gapn酶的核酸可源自与其在其中表达的工程改造的酵母相同的物种或生物体，或者可以源自与其在其中表达的工程改造的酵母不同的物种或生物体。
在一些实施方案中，编码gapn酶的重组核酸包含蜡样芽孢杆菌基因(例如，gapn，对应于uniprot号q2hqs1)。在一些实施方案中，编码gapn酶或其部分的重组核酸是密码子优化的。在一些实施方案中，编码gapn酶或其部分的重组核酸包含seqidno:110。
在一些实施方案中，编码gapn酶或其部分的重组核酸与seqidno:110的序列具有至少或约50％、至少或约60％、至少或约70％、至少或约75％、至少或约80％、至少或约81％、至少或约82％、至少或约83％、至少或约84％、至少或约85％、至少或约86％、至少或约87％、至少或约88％、至少或约89％、至少或约90％、至少或约91％、至少或约92％、至少或约93％、至少或约94％、至少或约95％、至少或约96％、至少或约97％、至少或约98％、至少或约99％、至少或约99.5％或至少或约99.9％的序列同一性。
在一些实施方案中，gapn蛋白包含seqidno：111。在一些实施方案中，gapn蛋白与seqidno:111的序列具有至少或约50％、至少或约60％、至少或约70％、至少或约75％、至少或约80％、至少或约81％、至少或约82％、至少或约83％、至少或约84％、至少或约85％、至少或约86％、至少或约87％、至少或约88％、至少或约89％、至少或约90％、至少或约91％、至少或约92％、至少或约93％、至少或约94％、至少或约95％、至少或约96％、至少或约97％、至少或约98％、至少或约99％、至少或约99.5％或至少或约99.9％的序列同一性。
本领域普通技术人员将理解gapn基因可源自任何来源，并且可以使用常规方法进行工程改造，诸如以改善在宿主细胞中的表达。另外，本领域普通技术人员将理解可在宿主细胞中的任何合适的基因座处插入gapn基因。
如本文中所述，在一个方面，ge酵母可包含多种遗传修饰，而在发酵期间中不表现出显著的发酵性能的变化和/或酵母细胞健康的变化。令人惊讶地发现，包含本文所述的海藻糖酶性状与表达葡糖淀粉酶的性状和/或甘油还原性状组合的ge酵母(即，与可比较的野生型酵母相比，产生更少和/或消耗更多甘油的ge酵母)可以达到比任何其他目前可用的产乙醇酵母菌株更高的乙醇滴度，而没有表现出与ge酵母性能相关的任何重大的负面影响。在一个方面，在ge酵母中包含本文所述的一种或多种遗传修饰不会负面影响发酵的性能特征。在一些实施方案中，未被显著影响的发酵性能特征包括但不限于：乙醇的平均生产速率；给定底物，例如给定的玉米醪上的最大乙醇滴度；用于ge酵母产生最大乙醇滴度的时间；以及对于ge酵母产生商业相关滴度(例如，至少110g/l、至少120g/l或至少130g/l)的时间。商业上相关的发酵时间，即可产生商业上相关的滴度同时使制造商获利的发酵周期时间可以根据特定的乙醇工厂而变化。然而，对于本公开的目的，商业上相关的发酵时间被认为是48小时或更短，40小时或更短或36小时或更短。与野生型菌株(诸如ethanolredtm)相比，不能在48小时内达到商业上相关的乙醇滴度和/或在48小时内的任何时间都表现出降低的乙醇生产速率的酵母菌株被认为表现出“发酵罚分(fermentationpenalty)”。在一些实施方案中，与商业上相关的野生型酵母菌株相比，本发明的ge酵母可表现出显著减少的发酵罚分和/或无统计学意义的发酵罚分。
在一些实施方案中，ge酵母包含编码甘油醛-3-磷酸脱氢酶(e.c.1.2.1.9)的重组核酸和减少的或消除的编码甘油-3-磷酸磷酸酶(e.c.3.1.3.21)的基因的表达，以及还有编码海藻糖酶的异源基因。在一些实施方案中，ge酵母包含编码甘油醛-3-磷酸脱氢酶(gapn，e.c.1.2.1.9)的重组核酸以及还有编码海藻糖酶的异源基因。在一些实施方案中，ge酵母可包含编码海藻糖酶的异源基因和编码葡糖淀粉酶的异源基因。
在一些实施方案中，ge酵母可包含编码海藻糖酶的异源基因；编码葡糖淀粉酶的异源基因；以及编码甘油醛-3-磷酸脱氢酶(e.c.1.2.1.9)的重组核酸和减少或消除的编码甘油-3-磷酸磷酸酶(e.c.3.1.3.21)的基因的表达。在一些实施方案中，ge酵母可包含编码海藻糖酶的异源基因；编码葡糖淀粉酶的异源基因；以及编码甘油醛-3-磷酸脱氢酶(e.c.1.2.1.9)的重组核酸。令人惊讶地表明，包含表达海藻糖酶的性状、表达葡糖淀粉酶的性状和/或甘油还原性状(例如，表达gapn的性状)的ge酵母可产生比其它酵母菌株显著更高量的乙醇，而不表现出通常与产乙醇酵母的遗传修饰相关的任何显著的负面性能特征。
在一些实施方案中，所述编码海藻糖酶的异源基因可以是编码与以下多肽序列中的任一个具有至少80％、85％、90％或95％的序列同一性的海藻糖酶(ec3.2.1.28)多肽的异源基因：seqidno：1(乳酸克鲁维酵母)、seqidno：2(近平滑假丝酵母)、seqidno：3(光滑假丝酵母)或seqidno：87(稻瘟病菌)。在一些实施方案中，ge酵母中编码葡糖淀粉酶的异源基因编码与以下多肽序列中的至少一个具有至少70％、75％、80％、85％、90％或95％的序列同一性的葡糖淀粉酶多肽：seqidno:16(sfga)、seqidno:17(roga)、seqidno:108(rmicga)或seqidno:109(德氏根霉(r.delemar)ga，即rdelga)。
ge酵母可包含本文所述的特定异源海藻糖酶基因和特定葡糖淀粉酶基因的任何组合。非限制性实例是包含来自稻瘟病菌的异源海藻糖酶基因和来自小孢根霉的异源ga基因的ge酵母；包含来自稻瘟病菌的异源海藻糖酶基因和来自扣囊复膜酵母菌的异源ga基因的ge酵母；包含来自稻瘟病菌的异源海藻糖酶基因和来自戴尔根霉的异源ga基因的ge酵母；包含来自稻瘟病菌的异源海藻糖酶基因和来自米根霉的异源ga基因的ge酵母；包含来自光滑假丝酵母的异源海藻糖酶基因和来自小孢根霉的异源ga基因的ge酵母；包含来自光滑假丝酵母的异源海藻糖基因和来自扣囊复膜酵母菌的异源ga基因的ge酵母；包含来自近平滑假丝酵母的异源海藻糖酶基因和来自小孢根霉的异源ga基因的ge酵母；包含来自近平滑假丝酵母的异源海藻糖基因和来自扣囊复膜酵母菌的异源ga基因的ge酵母以胶包含来自近平滑假丝酵母的异源海藻糖基因和来自戴尔根霉的异源ga基因的ge酵母。另外，ge酵母的任何上述实例(和本文提供的ge酵母的任何其它实例)也可包括如本文所述的甘油还原(gr)的形式。应当理解，在本文所述的任何实施方案中，当本公开提及包含来自某些物种的异源基因的ge酵母时，ge酵母中的此类基因将编码与该基因相关的多肽(在本申请的序列表中提供了此类多肽，包括具有和不具有天然前导序列或具有非天然前导序列的此类多肽的形式的非限制性实例)。
应当理解，ge酵母可包含本文描述的性状的任何组合。例如，ge酵母可表达任何海藻糖酶，也可表达任何葡糖淀粉酶，并且还可包含一种或多种甘油还原性状的任何形式。除了这三个性状(或这些性状中的两个的任何组合)以外，ge酵母还可表达任何异麦芽糖酶和/或可包含消耗乳酸的性状。令人惊奇地发现，ge酵母能够包含本文所述的每种性状的形式而不会表现出显著的发酵罚分。
发酵方法
在一个方面，本发明涉及发酵方法。在一个方面，发酵方法可以是使用本文描述的经遗传修饰的酵母的实施方案来产生发酵产物的任何方法。在一些实施方案中，发酵产物是乙醇。在一些实施方案中，发酵产物可以是除乙醇外的发酵产物，例如但不限于正丙醇、异丙醇、正丁醇、异丁醇、丁二烯或异戊二烯。
示例性的发酵方法可包括以下步骤：提供包含碳源的发酵培养基，将酵母添加到发酵培养基中，用酵母发酵该培养基以产生生物产物，以及收获该生物产物。在一个方面，培养基中的碳源可包括淀粉、糖、有机酸或其混合物。在一些实施方案中，糖可包括海藻糖。在一些实施方案中，培养基可包含乳酸盐。
在一个方面，如本领域技术人员将理解的，培养基的组成在发酵期间可以变化。例如，葡萄糖或另一种己糖可在发酵期间通过酶活性由寡聚物产生，然后被消耗掉。因此，在一些实施方案中，如果葡萄糖被酵母消耗比其从葡萄糖寡聚物产生更快，则葡萄糖含量在发酵的某些点可能非常低，甚至无法检测。在一些实施方案中，例如分批补料发酵，可以向所述培养基连续或半连续地补充进料流，诸如蔬菜加工进料流。
因此，在一个方面，用于本文描述的方法的发酵培养基的各种组分的浓度可以是平均浓度。组分的平均浓度可通过本领域中已知的方法来计算，例如通过获取发酵开始时的发酵培养基中组分的浓度和发酵结束时的发酵培养基中相同组分的浓度的平均值来计算。平均值的这种计算还可考虑发酵方法过程中任何输入和/或输出流中组分的浓度。另外，对于连续发酵方法，组分的平均浓度可以指任何单个容器中的平均浓度，或者其可以指整个过程中的平均浓度，即，考虑到过程的所有进料流和所有输出流。
在一些实施方案中，至少5％、10％、15％、20％、25％、30％、35％、35％、40％、45％、50％、55％、60％、65％、70％、75％、80％、85％、90％、95％或99％的存在于发酵培养基中和/或在发酵期间由酵母产生的海藻糖在发酵结束时被消耗掉。
在一些实施方案中，发酵结束时发酵培养基中海藻糖的量在0至10g/l、0至5g/l、0至4g/l、0至3g/l、0至1g/l、0至0.5g/l、0至0.1g/l、0.001至3g/l、0.001至1g/l或0.001至0.1g/l的范围内。在一些实施方案中，发酵结束时发酵培养基中海藻糖的量在0.5至5g/l、1至5g/l、0.5至4g/l、0.5至3g/l、0.5至2g/l或0.1至2g/l的范围内。在一些实施方案中，发酵结束时发酵培养基中总海藻糖的量小于10g/l、7.5g/l、5g/l、4g/l、3g/l、2g/l、1g/l、0.5g/l或0.1g/l。
在一些实施方案中，至少0.1g/l、0.5g/l、1g/l、2g/l、3g/l、4g/l、5g/l、6g/l、7g/l、8g/l、9g/l或10g/l海藻糖在此过程中被转化成葡萄糖。与海藻糖转化成葡萄糖有相关的上述参数涉及不包括外源海藻糖酶的过程，即海藻糖的转化是由ge酵母表达的海藻糖酶进行的。在一些实施方案中，在本文描述的任何方法中海藻糖向葡萄糖的转化还与大量乙醇产生相关，例如至少40g/l、45g/l、50g/l、55g/l、60g/l、65g/l、70g/l、75g/l、80g/l、85g/l、90g/l、95g/l、100g/l、105g/l、110g/l、115g/l、120g/l、125g/l、130g/l或135g/l。另外，在一个方面，本发明的方法可用于以商业上显著的速率和/或滴度生产乙醇。在一些实施方案中，乙醇的产生速率可以是1至6gl-1h-1、1至5.5gl-1h-1或1至5gl-1h-1。
在一些实施方案中，可以将外源酶添加到该过程中。例如，在一些实施方案中，可以将外源海藻糖酶、葡糖淀粉酶和/或异麦芽糖酶添加到发酵方法中。
在目前的发酵方法中，海藻糖的来源主要来自酵母本身。最初的发酵液中可能存在少量海藻糖，但是，大多数可能是由酵母产生的。由酵母产生的海藻糖的量与对酵母的应激水平相关。因此，产生大量乙醇的过程，诸如本发明的过程，可对酵母造成乙醇相关的应激，从而增加存在于发酵液中的海藻糖的量。诱导酵母合成海藻糖的应激也可由其它因素诸如热或升高的盐浓度引起。在一些实施方案中，使用纤维素制品作为底物进行的发酵方法与大量的海藻糖形成相关。这些纤维素乙醇过程可诱导更多对酵母的应激。因此，本文所述的酵母对于使用纤维素材料作为主要发酵底物的发酵方法可以是特别有利的，或者除了葡萄糖或葡萄糖低聚物以外还具有大量的纤维素材料。
分批发酵方法
在一个方面，本发明的方法可以是分批发酵方法。在一些实施方案中，本发明的分批方法是干磨或干磨乙醇生产方法。分批发酵方法，包括干磨乙醇方法是本领域公知的。
示例性分批发酵方法包括以下步骤：提供含有碳源诸如碳水化合物的发酵培养基，并使用本文所述类型的遗传工程改造的酵母使该培养基发酵。在一些实施方案中，酵母包含异源海藻糖酶基因。在一些实施方案中，在发酵开始时，培养基包含浓度为至少0.5g/l、1g/l、2g/l或3g/l的葡萄糖或葡萄糖寡聚物。
连续发酵方法
在一个方面，本发明的方法可以是连续的或半连续的发酵方法。在一些实施方案中，本发明的连续方法是湿法玉米研磨乙醇生产方法。包括湿磨乙醇方法在内的连续发酵方法是本领域中公知的。在一些实施方案中，具有连续操作模式的发酵方法包括多个串联操作的发酵罐，其中在第一发酵罐中供应淀粉水解产物，将淀粉水解产物供给第二发酵罐，依此类推，直到淀粉水解产物转化为乙醇。在一些实施方案中，可使用1至10个或2至7个发酵罐进行连续操作。在一些实施方案中，可在单个容器中进行连续发酵方法，其中可按照连续或半连续的时间表添加原料并且可以去除含有产物的发酵液。
用于生产乙醇的示例性连续发酵方法包括以下步骤：提供包含葡萄糖或葡萄糖寡聚物的初始发酵培养基，用经遗传修饰的酵母使发酵培养基发酵，以及从发酵培养基中除去至少一种包含乙醇的输出流。
在一些实施方案中，将初始发酵培养基添加至预发酵罐或生长发酵罐容器中，在其中添加经遗传修饰的酵母并使其生长直至获得所需的生物质。在一些实施方案中，将预发酵罐中的过程条件设置为有利于细胞生长而非发酵产物形成。在一些实施方案中，然后可以将预发酵罐容器中的内容物转移至第二发酵罐容器中。在第二个发酵罐容器中，将过程条件设置为有利于发酵产物的形成而非细胞生长。在一些实施方案中，以单部分或以连续或半连续的方式将另外的发酵培养基添加到第二发酵罐容器中。在一些实施方案中，添加至第二发酵罐容器的另外的发酵培养基含有乳酸和/或其它碳源。上面提及的第二发酵罐也可以称为“培育箱(propagator)”。在一些实施例中，第二发酵罐容器的内容物可被转移到第三发酵罐容器。第三发酵罐容器的过程条件可以与第二发酵罐容器相同或不同。在一些实施方案中，可将第三发酵罐容器的内容物转移至一个或多个另外的发酵罐容器，如连续发酵方法领域的技术人员所理解的。在一些实施方案中，从最终发酵罐容器的内容物中分离出生物产物，例如乙醇。
在一些实施方案中，预发酵罐容器中发酵培养基的平均葡萄糖浓度在10至20g/l的范围内。在一些实施方案中，第二发酵罐容器中发酵培养基的平均葡萄糖浓度在30至40g/l的范围内。在一些实施方案中，第三发酵罐容器或任何另外的发酵罐容器中发酵培养基的平均葡萄糖浓度在30至40g/l的范围内。在一些实施方案中，最终发酵罐容器中发酵培养基的平均葡萄糖浓度在0至5g/l的范围内。在一些实施方案中，预发酵罐容器、培育箱容器中或任何发酵容器中的发酵培养基的平均葡萄糖浓度在0-5g/l、2-5g/l、1-10g/l、5-10g/l、5-15g/l、5-20g/l、10-20g/l、15-25g/l、20-30g/l、25-35g/l、30-40g/l或35-45g/l的范围内。在一些实施方案中，将预发酵罐容器、培育箱容器中或任何发酵容器中的发酵物的平均葡萄糖浓度维持在大于或等于与酵母中的葡萄糖阻抑相关的葡萄糖浓度的范围内。在一些实施方案中，酵母中与葡萄糖阻抑相关的葡萄糖浓度在2至5g/l的范围内。因此，在此类实施方案中，可将预发酵罐容器中或任何发酵容器中的发酵培养基的平均葡萄糖浓度维持在大于或等于2g/l、3g/l、4g/l或5g/l的水平。
可以在连续发酵方法中调节和/或维持其它发酵条件，包括但不限于：温度、ph、体积或比耗氧速率(our)或任何碳源或任何发酵培养基营养物的浓度。在一些实施方案中，预发酵罐容器、培育箱容器中或任何其它发酵容器中的温度可在20-45℃、20-40℃、20-30℃、25-35℃或30-40℃的范围内。在一些实施方案中，预发酵罐、培育箱容器或中任何其它发酵容器中的ph可在2至7、3至6、4.5至5.5或3.5至4.5的范围内。
在一些实施方案中，预发酵罐容器中的细胞密度在3至10g/l或5至10g/l的范围内。在一些实施方案中，培育箱容器中的细胞密度在10至50g/l的范围内。
在一个方面，所述方法使用本文所述的酵母，其在发酵方法期间消耗通过使用海藻糖酶的催化从海藻糖产生的葡萄糖。在一些实施方案中，所有输出流的总和中的总海藻糖含量小于添加到发酵方法中或在发酵方法中产生的海藻糖酶的90％。在一些实施方案中，发酵方法的输出中的总海藻糖含量小于发酵方法期间产生的海藻糖的99％、95％、85％、80％、75％、70％、65％、60％、55％、50％、45％、40％、35％、30％、25％、20％、15％或10％。在一些实施方案中，发酵方法的所有输出流的总和中的海藻糖含量小于10g/l、9g/l、8g/l、7g/l、6g/l、5g/l、4g/l、3g/l、2g/l、1g/l、0.5g/l或0.1g/l。
本文所述的连续发酵方法可以以商业上显着的速率产生乙醇或另一种生物产物。在一些实施方案中，所述方法可以以至少0.5、1.0、1.5、2.0、2.5、3.0或3.2gl-1h-1的速率产生生物产物。
实验实施例
通过参考以下实验实施例进一步详细地描述本发明。除非另外指明，否则提供这些实施例仅仅是为了说明的目的，并非旨在为限制性的。因此，决不应将本发明解释为限于以下实施例，相反，应解释为涵盖由于本文提供的教导而变得显而易见的任何及所有变型。本公开总体上涉及产生乙醇的酿酒酵母的实施方案。然而，本公开不限于此类酵母和发酵产物。针对其它酵母种和/或生物产物的实施方案旨在包括在本公开的教导和发明内。
实施例1：表达海藻糖酶的经遗传修饰的酵母菌株
在本实施例中，酿酒酵母菌株被转化为表达来自乳酸克鲁维酵母、近光滑假丝酵母和光滑假丝酵母的酸性海藻糖酶。使用tdh3启动子表达酸性海藻糖酶。每种海藻糖酶的形式用该基因的天然信号肽合成，并且每种海藻糖酶的第二形式用来自酿酒酵母的mfα2信号肽合成。酿酒酵母菌株也被转化为表达使用sam2启动子表达的来自稻瘟病菌的海藻糖酶。所选菌株也被转化为包含葡糖淀粉酶表达和/或与甘油还原相关的基因。转化的菌株用于产生乙醇的发酵中。转化的菌株消耗发酵中形成的海藻糖，并且与不表达异源海藻糖酶的菌株相比，转化的菌株经显示产生更高量的乙醇。
菌株构建
菌株1
2016年3月25日提交的国际专利申请公开号wo2016/160584中描述的菌株1-3是酿酒酵母菌株(菌株14883，ethanolredtm酿酒酵母的形式)，其中两个scura3基因拷贝均被缺失。对于本公开的目的，菌株1-3被称为菌株1。
菌株2
用seqidno：6转化菌株1。seqidno：6包含：i)包含sctdh3启动子的空表达盒；ii)scura3表达盒；iii)用于稳定复制的酿酒酵母cen6着丝粒；和iv)β-内酰胺酶表达盒。在scd-uracil板上选择转化体。将所得转化体在scd-uracil板上划线进行单菌落分离。选择单菌落。通过pcr验证seqidno：6至选菌落中的正确整合。将pcr验证的分离株命名为菌株2。
菌株3
用seqidno：7转化菌株1。seqidno：7包含：i)具有mfα2分泌信号的来自乳酸克鲁维酵母的海藻糖酶的表达盒，其编码由tdh3启动子表达的氨基酸序列seqidno：8；ii)scura3表达盒；iii)用于稳定复制的酿酒酵母cen6着丝粒；和iv)β-内酰胺酶表达盒。在scd-uracil板上选择转化体。将所得转化体在scd-uracil板上划线进行单菌落分离。选择单菌落。通过pcr验证seqidno:7至所选菌落中的正确整合。将pcr验证的分离株命名为菌株3。
菌株4
用seqidno：9转化菌株1。seqidno：9包含：i)来自乳酸克鲁维酵母的海藻糖酶的表达盒，其编码由tdh3启动子表达的氨基酸序列seqidno：1；ii)scura3表达盒；iii)用于稳定复制的酿酒酵母cen6着丝粒；和iv)β-内酰胺酶表达盒。在scd-uracil板上选择转化体。将所得转化体在scd-uracil板上划线进行单菌落分离。选择单菌落。通过pcr验证seqidno:9至所选菌落中的正确整合。将pcr验证的分离株命名为菌株4。
菌株5
用seqidno：10转化菌株1。seqidno：10包含：i)具有mfα2分泌信号的来自近平滑假丝酵母的海藻糖酶的表达盒，其编码由tdh3启动子表达的氨基酸序列seqidno：11；ii)scura3表达盒；iii)用于稳定复制的酿酒酵母cen6着丝粒；和iv)β-内酰胺酶表达盒。在scd-uracil板上选择转化体。将所得转化体在scd-uracil板上划线进行单菌落分离。选择单菌落。通过pcr验证seqidno:10至所选菌落中的正确整合。将pcr验证的分离株命名为菌株5。
菌株6
用seqidno：12转化菌株1。seqidno：12包含：i)来自近平滑假丝酵母的海藻糖酶的表达盒，其编码由tdh3启动子表达的氨基酸序列seqidno：2；ii)scura3表达盒；iii)用于稳定复制的酿酒酵母cen6着丝粒；和iv)β-内酰胺酶表达盒。在scd-uracil板上选择转化体。将所得转化体在scd-uracil板上划线进行单菌落分离。选择单菌落。通过pcr验证seqidno:12至所选菌落中的正确整合。将pcr验证的分离株命名为菌株6。
菌株7
用seqidno：13转化菌株1。seqidno：13包含：i)具有mfα2分泌信号的来自光滑假丝酵母的海藻糖酶的表达盒，其编码由tdh3启动子表达的氨基酸序列seqidno：14；ii)scura3表达盒；iii)用于稳定复制的酿酒酵母cen6着丝粒；和iv)β-内酰胺酶表达盒。在scd-uracil板上选择转化体。将所得转化体在scd-uracil板上划线进行单菌落分离。选择单菌落。通过pcr验证seqidno:13至所选菌落中的正确整合。将pcr验证的分离株命名为菌株7。
菌株8
用seqidno：15转化菌株1。seqidno：15包含：i)来自光滑假丝酵母的海藻糖酶的表达盒，其编码由tdh3启动子表达的氨基酸序列seqidno：3；ii)scura3表达盒；iii)用于稳定复制的酿酒酵母cen6着丝粒；和iv)β-内酰胺酶表达盒。在scd-uracil板上选择转化体。将所得转化体在scd-uracil板上划线进行单菌落分离。选择单菌落。通过pcr验证seqidno:15至所选菌落中的正确整合。将pcr验证的分离株命名为菌株8。
菌株9
用seqidno:93和seqidno:94共转化菌株1。seqidno:93含有以下元件：i)与天然fcy1基因的5'区同源的dna；和ii)小孢根霉葡糖淀粉酶的独特密码子优化的变体(seqidno:95)的表达盒，其处于tdh3启动子和cyc1终止子的控制下；以及iii)ura3启动子以及ura3基因的一部分。seqidno：94包含以下元件：i)ura3基因的一部分和终止子；以及ii)与天然fcy1基因的3'区域同源的dna。在scd-ura上选择转化体。将所得转化体在scd-ura上划线进行单菌落分离。选择单个菌落，并通过pcr确认表达盒的正确整合。在摇瓶发酵中测试了三个独立的转化体，并且将代表性分离株命名为菌株9。
菌株10
用seqidno:96和seqidno:97共转化菌株9。seqidno:96含有以下元件：i)与天然fcy1基因的5’区同源的dna；和ii)小孢根霉葡糖淀粉酶的独特密码子优化的变体(seqidno:95)的表达盒，其处于tdh3启动子和cyc1终止子的控制下；以及iii)tef1启动子以及构巢曲霉(aspergillusnidulans)amds基因的一部分。seqidno：97包含以下元件：i)构巢曲霉乙酰胺酶(amds)基因的一部分和tef1终止子；以及ii)与天然fcy1基因的3'区域同源的dna。在ynb+乙酰胺板上选择转化体。将所得转化体在ynb+乙酰胺板上划线进行单菌落分离。选择单个菌落，并通过pcr确认表达盒的正确整合。在摇瓶发酵中测试了三个独立的转化体，并且将代表性分离株命名为菌株10。
菌株11
用seqidno：98转化菌株10。在含有3.5g/l对氟苯丙氨酸和1g/ll-酪氨酸(scd-pfp)的合成完全培养基上选择转化体。将所得转化体在scd-pfp上划线进行单菌落分离。选择单菌落。将pcr验证的分离株命名为菌株11。
菌株12
用seqidno：99转化菌株11。在scd-ura上选择转化体。将所得转化体在scd-ura上划线进行单菌落分离。选择单菌落。将pcr验证的分离株命名为菌株12。
菌株13
用seqidno:100和seqidno:101以及seqidno:102和seqidno:103共转化菌株11。在ynb+乙酰胺板上选择转化体。将所得转化体在ynb+乙酰胺板上划线进行单菌落分离。选择单个菌落，并通过测序确认表达盒的正确整合。在摇瓶发酵中测试了三个独立的转化体，并且将代表性分离株命名为菌株13。
菌株14
用seqidno：98转化菌株13。在含有3.5g/l对氟苯丙氨酸和1g/ll-酪氨酸(scd-pfp)的合成完全培养基上选择转化体。将所得转化体在scd-pfp上划线进行单菌落分离。选择单菌落。将pcr验证的分离株命名为菌株14。
菌株15
用seqidno：99转化菌株14。在scd-ura上选择转化体。将所得转化体在scd-ura上划线进行单菌落分离。选择单菌落。将pcr验证的分离株命名为菌株15。
菌株16
用seqidno:93和seqidno:104共转化菌株11。seqidno:93含有以下元件：i)与天然fcy1基因的5'区同源的dna；和ii)小孢根霉葡糖淀粉酶的独特密码子优化的变体(seqidno:95)的表达盒，其处于tdh3启动子和cyc1终止子的控制下；以及iii)ura3启动子以及ura3基因的一部分。seqidno：104包含以下元件：i)ura3基因的一部分和终止子；和ii)具有mfα2分泌信号的稻瘟病菌海藻糖酶的密码子优化的变体(seqidno：105)的表达盒，其处于sam2启动子(seqidno：112)和gal10终止子的控制下；以及iii)与天然fcy1基因的3'区同源的dna。在scd-ura上选择转化体。将所得转化体在scd-ura上划线进行单菌落分离。选择单个菌落，并通过pcr确认表达盒的正确整合。在摇瓶发酵中测试了三个独立的转化体，并且将代表性分离株命名为菌株16。
菌株17
用seqidno:96和seqidno:105共转化菌株16。seqidno:96含有以下元件：i)与天然fcy1基因的5'区同源的dna；和ii)小孢根霉葡糖淀粉酶的独特密码子优化的变体(seqidno:95)的表达盒，其处于tdh3启动子和cyc1终止子的控制下；以及iii)tef1启动子以及构巢曲霉amds基因的一部分。seqidno：105包含以下元件：i)构巢曲霉乙酰胺酶(amds)基因的一部分和adh1终止子；和ii)具有mfα2分泌信号的稻瘟病菌海藻糖酶的密码子优化的变体(seqidno：105)的表达盒，其处于sam2启动子(seqidno：112)和gal10终止子的控制下；以及iii)与天然fcy1基因的3'区同源的dna。在ynb+乙酰胺板上选择转化体。将所得转化体在ynb+乙酰胺板上划线进行单菌落分离。选择单个菌落，并通过pcr确认表达盒的正确整合。在摇瓶发酵中测试了三个独立的转化体，并且将代表性分离株命名为菌株17。
菌株18
用seqidno：98转化菌株17。在含有3.5g/l对氟苯丙氨酸和1g/ll-酪氨酸(scd-pfp)的合成完全培养基上选择转化体。将所得转化体在scd-pfp上划线进行单菌落分离。选择单菌落。将pcr验证的分离株命名为菌株18。
菌株19
用seqidno：99转化菌株18。在scd-ura上选择转化体。将所得转化体在scd-ura上划线进行单菌落分离。选择单菌落。将pcr验证的分离株命名为菌株19。
菌株20
用seqidno:93和seqidno:106共转化菌株11。seqidno:93含有以下元件：i)与天然fcy1基因的5'区同源的dna；和ii)小孢根霉葡糖淀粉酶的独特密码子优化的变体(seqidno:95)的表达盒，其处于tdh3启动子和cyc1终止子的控制下；以及iii)ura3启动子以及ura3基因的一部分。seqidno：106含有以下元件：i)ura3基因的一部分和终止子；和ii)具有mfα2分泌信号的光滑假丝酵母海藻糖酶的密码子优化的变体(seqidno：14)的表达盒，其处于sam2启动子(seqidno：112)和gal10终止子的控制下；以及iii)与天然fcy1基因的3'区同源的dna。在scd-ura上选择转化体。将所得转化体在scd-ura上划线进行单菌落分离。选择单个菌落，并通过pcr确认表达盒的正确整合。在摇瓶发酵中测试了三个独立的转化体，并且将代表性分离株命名为菌株20。
菌株21
用seqidno:96和seqidno:107共转化菌株20。seqidno:96含有以下元件：i)与天然fcy1基因的5'区同源的dna；和ii)小孢根霉葡糖淀粉酶的独特密码子优化的变体(seqidno:95)的表达盒，其处于tdh3启动子和cyc1终止子的控制下；以及iii)tef1启动子以及构巢曲霉amds基因的一部分。seqidno：107含有以下元件：i)构巢曲霉乙酰胺酶(amds)基因的一部分和adh1终止子；和ii)具有mfα2分泌信号的光滑假丝酵母海藻糖酶的密码子优化的变体(seqidno：14)的表达盒，其处于sam2启动子(seqidno：112)和gal10终止子的控制下；以及iii)与天然fcy1基因的3'区同源的dna。在ynb+乙酰胺板上选择转化体。将所得转化体在ynb+乙酰胺板上划线进行单菌落分离。选择单个菌落，并通过pcr确认表达盒的正确整合。在摇瓶发酵中测试了三个独立的转化体，并且将代表性分离株命名为菌株21。
菌株22
用seqidno:93和seqidno:104共转化菌株14。seqidno:93含有以下元件：i)与天然fcy1基因的5'区同源的dna；和ii)小孢根霉葡糖淀粉酶的独特密码子优化的变体(seqidno:95)的表达盒，其处于tdh3启动子和cyc1终止子的控制下；以及iii)ura3启动子以及ura3基因的一部分。seqidno：104含有以下元件：i)ura3基因的一部分和终止子；和ii)具有mfα2分泌信号的稻瘟病菌海藻糖酶的密码子优化的变体(seqidno：92)的表达盒，其处于sam2启动子(seqidno：112)和gal10终止子的控制下；以及iii)与天然fcy1基因的3'区同源的dna。在scd-ura上选择转化体。将所得转化体在scd-ura上划线进行单菌落分离。选择单个菌落，并通过pcr确认表达盒的正确整合。在摇瓶发酵中测试了三个独立的转化体，并且将代表性分离株命名为菌株22。
菌株23
用seqidno:96和seqidno:105共转化菌株22。seqidno:96含有以下元件：i)与天然fcy1基因的5'区同源的dna；和ii)小孢根霉葡糖淀粉酶的独特密码子优化的变体(seqidno:95)的表达盒，其处于tdh3启动子和cyc1终止子的控制下；以及iii)tef1启动子以及构巢曲霉amds基因的一部分。seqidno：105含有以下元件：i)构巢曲霉乙酰胺酶(amds)基因的一部分和adh1终止子；和ii)具有mfα2分泌信号的稻瘟病菌海藻糖酶的密码子优化的变体(seqidno：92)的表达盒，其处于sam2启动子(seqidno：112)和gal10终止子的控制下；以及iii)与天然fcy1基因的3'区同源的dna。在ynb+乙酰胺板上选择转化体。将所得转化体在ynb+乙酰胺板上划线进行单菌落分离。选择单个菌落，并通过pcr确认表达盒的正确整合。在摇瓶发酵中测试了三个独立的转化体，并且将代表性分离株命名为菌株23。
表1.菌株的描述
实施例2：在摇瓶测定中表征菌株
摇瓶法
将菌株2-8划线至scd-ura板并且在30℃下孵育直至单菌落可见(1-2天)。将来自scd-ura板的细胞刮入无菌摇瓶培养基中，并且测量光密度(od600)。使用genesys20型分光光度计(thermoscientific)在600nm波长和1cm路径长度下测量光密度。用细胞浆液接种摇瓶以达到0.1-0.3的初始od600。在即将接种之前，将50ml摇瓶培养基添加到250ml无挡板摇瓶(corning4995-250)中，该摇瓶装配有含有透气密封件(corning1395-45ltmc)的螺纹盖。摇瓶培养基由725g部分水解的玉米淀粉、150g过滤后的轻浸渍水、50g水、25g葡萄糖和1g尿素组成。将每种菌株的一式二份烧瓶在30℃和80％湿度下在定轨振荡器中以100rpm振荡孵育48小时。取样并通过高效液相色谱(hplc)分析发酵期间发酵液中的乙醇和海藻糖的浓度。
结果
在每个图中，对照菌株为菌株2；含有来自乳酸克鲁维酵母的异源海藻糖酶的菌株是菌株3和4；含有来自近平滑假丝酵母的异源海藻糖酶的菌株是菌株5和6；以及含有来自光滑假丝酵母的异源海藻糖酶的菌株是菌株7和8。
图1显示在不添加海藻糖的发酵中，含有来自近平滑假丝酵母的异源海藻糖酶的菌株比对照菌株消耗显著更多的在发酵期间产生的海藻糖。
图2和图3显示在不添加海藻糖的发酵中，含有来自近平滑假丝酵母的异源海藻糖酶的菌株比对照菌株产生显著更多的乙醇。
图4显示，在开始前添加10g/l海藻糖的发酵中，含有来自乳酸克鲁维酵母(3&4)、近平滑假丝酵母(5&6)和光滑假丝酵母(7&8)的异源海藻糖酶的菌株比对照菌株(2)消耗显著更多的海藻糖。同样，与使用相应基因的天然信号肽的相同基因相比，利用mfα2信号肽的所有3种异源海藻糖酶均以比利用各自基因的天然信号肽的同一基因更快的速率水解海藻糖。含有近平滑假丝酵母和光滑假丝酵母异源海藻糖酶的菌株全部均达到约1g/l的最终海藻糖滴度，导致如图5和图6中显示的相似的48小时乙醇滴度。
图5和图6显示，在开始前添加10g/l海藻糖的发酵中，含有来自乳酸克鲁维酵母、近平滑假丝酵母和光滑假丝酵母的异源海藻糖酶的菌株比对照菌株产生显著更多的乙醇。含有近平滑假丝酵母和光滑假丝酵母异源海藻糖酶的菌株比含有乳酸克鲁维酵母海藻糖酶的菌株产生显著更多的乙醇。
图7显示，在即将在40小时取样之前向发酵中添加10g/l海藻糖的发酵中，含有来自近平滑假丝酵母的异源海藻糖酶的菌株比对照菌株消耗显著更多的海藻糖。数据显示，海藻糖酶在发酵后期仍具有显著活性。
图8和图9显示，在即将在40小时取样之前向发酵中添加10g/l海藻糖的发酵中，含有来自近平滑假丝酵母的异源海藻糖酶的菌株比对照菌株产生显著更多的乙醇。
实施例3：在33.3℃下在32％的ds玉米醪中菌株的表征
将菌株12、15、19、21、23和24划线至ypd板并且在30℃下孵育直至单菌落可见(1-2天)。将来自ypd板的细胞刮入ph7.0的磷酸盐缓冲液中，并测量光密度(od600)。使用genesys20型分光光度计(thermoscientific)在600nm波长和1cm路径长度下测量光密度。用细胞浆液接种摇瓶以达到为0.1的初始od600。在即将接种之前，将以下材料添加到每个烧瓶中：将50克液化玉米醪添加到250ml带挡板的摇瓶(该摇瓶用气锁(air-lock)密封)中，所述摇瓶含有4ml无菌芥花油、190ul500g/l过滤灭菌的尿素和2.5ul100mg/ml过滤灭菌的氨苄青霉素。将0.284agu/gds(70μl的1:2稀释液)的葡糖淀粉酶(来自黑曲霉的淀粉葡糖苷酶，sigma)添加到含有对照菌株24的烧瓶中，并将0.114agu/gds(28μl的1:2稀释液)的葡糖淀粉酶(来自黑曲霉的淀粉葡糖苷酶，sigma)添加到剩余的烧瓶中。来自黑曲霉的淀粉葡萄糖苷酶(sigma(目录号a7095))据估计具有约260agu/ml的酶水溶液。将每种菌株的一式二份烧瓶在33.3℃下在定轨振荡器中以100rpm振荡孵育约48小时。在48小时时，获取1ml样品，并通过高效液相色谱(hplc)分析其发酵液中的乙醇和海藻糖的浓度。
图10和图11显示了玉米醪发酵中所选菌株的结果。含有异源海藻糖酶的菌株(菌株19、21和23)以及还有小孢根霉ga(rmicga)与野生型菌株(菌株24)或具有rmicga而无异源海藻糖酶的菌株(菌株12)相比，在发酵结束时显示出显著更高的乙醇产量和显著更低的存在的海藻糖。菌株15(其包含rmicga和甘油还原性状两者，但不包含异源海藻糖酶)显示出比一些包含异源海藻糖酶的菌株更高的乙醇滴度。然而，具有与菌株15相同的性状而且还包含异源海藻糖酶的相应菌株(菌株23)，显示出在所有测试菌株中最高的乙醇滴度。
实施方案
提供以下实施方案作为实施方案的非限制性实例。本申请不仅仅限于这些实施方案。
实施方案a.一种经遗传修饰的酵母，其包含编码海藻糖酶(ec3.2.1.28)多肽的异源基因，
其中当酵母存在于包含海藻糖的发酵培养基中时，酵母能够产生乙醇。
b.如实施方案a的酵母，其中所述海藻糖酶多肽是酸性海藻糖酶。
c.如实施方案a-b中任一项的酵母，其中编码海藻糖酶多肽的基因源自选自由以下组成的组的生物体：稻瘟病菌、乳酸克鲁维酵母、近平滑假丝酵母和光滑假丝酵母。
d.如实施方案a-c中任一项的酵母，其中由酵母编码的海藻糖酶多肽与以下多肽序列中的至少一个具有至少75％、80％、85％、90％、95％或97％的序列同一性：seqidno：1、seqidno：2、seqidno：3或seqidno：87。
e.如实施方案a-d中任一项的酵母，其中由酵母编码的海藻糖酶多肽包含与seqidno：83具有至少70％、80％、90％或95％的序列同一性的序列。
f.如实施方案a-e中任一项的酵母，其中由酵母编码的海藻糖酶多肽包含与seqidno：84和/或seqidno：85具有至少70％、至少75％、至少80％、至少85％、至少90％、至少94％或100％的序列同一性的序列。
g.如实施方案a-f中任一项的酵母，其中所述酵母是经遗传修饰的酿酒酵母。
h.如实施方案a-g中任一项的酵母，其中由所述酵母编码的海藻糖酶包含mfα2信号序列。
i.如实施方案h的酵母，其中所述mfα2信号序列是seqidno：4。
j.如实施方案h的酵母，其中所述mfα2信号序列与seqidno：4具有至少84％、89％或94％的序列同一性。
k.如实施方案a-j中任一项的酵母，其中由酵母编码的海藻糖酶与以下多肽序列中的至少一个具有至少75％、80％、85％、90％、95％或97％的序列同一性：seqidno：8、seqidno：11、seqidno：14或seqidno：92。
l.如实施方案a-k中任一项的酵母，其还包含编码葡糖淀粉酶(ec3.2.1.3)多肽的异源基因。
m.如实施方案l的酵母，其中编码葡糖淀粉酶多肽的异源基因是源自选自由以下组成的组的物种的葡糖淀粉酶基因：树脂枝胞霉、黑曲霉、泡盛曲霉、米曲霉、白曲霉、白宇佐美曲霉、食腺嘌呤芽生葡萄孢酵母、白假丝酵母、米根霉、粟酒裂殖酵母、扣囊复膜酵母菌布鲁塞尔酒香酵母和产朊假丝酵母。
n.如实施方案l的酵母，其中由酵母编码的葡糖淀粉酶多肽与以下多肽序列中的至少一个具有至少70％、75％、80％、85％、90％或95％的序列同一性：seqidno：16、seqidno：17、seqidno：18或seqidno：19。
o.如实施方案a-n中任一项的酵母，其还包含编码异麦芽糖酶(ec3.2.1.10)多肽的异源基因。
p.如实施方案a-o中任一项的酵母，其中所述酵母编码与seqidno：20的多肽具有至少70％、80％、90％或95％的序列同一性的糖转运蛋白多肽。
q.如实施方案a-o中任一项的酵母，其中所述酵母编码与seqidno：21的多肽具有至少70％、80％、90％或95％的序列同一性的糖转运蛋白多肽。
r.如实施方案a-q中任一项的酵母，其还包含编码细胞色素b2(cyb2)(ec1.1.2.3)多肽的异源基因。
s.如实施方案r的酵母，其中所述cyb2多肽具有氨基酸序列，所述氨基酸序列与以下氨基酸序列中的任一个具有至少50％、55％、65％、70％、75％、80％、85％、90％、95％、97％或99％的序列同一性：seqidno:27、seqidno:28、seqidno:29、seqidno:30、seqidno:31、seqidno:32或seqidno:33。
t.如实施方案r的酵母，其中所述cyb2多肽在seqidno:27、seqidno:28、seqidno:29、seqidno:30、seqidno:31、seqidno:32或seqidno:33中指定的位置处包含以下残基中的一个或多个：lys349、tyr143、tyr254和his373。
u.如实施方案a-t中任一项的酵母，其还包含编码d-乳酸脱氢酶(dld)(ec1.1.2.4)多肽的异源基因。
v.如实施方案u的酵母，其中所述编码的dld多肽具有氨基酸序列，所述氨基酸序列与以下氨基酸序列中的任一个具有至少50％、55％、65％、70％、75％、80％、85％、90％、95％、97％或99％的序列同一性：seqidno:34、seqidno:35、seqidno:36、seqidno:37、seqidno:38、seqidno:39、seqidno:40或seqidno:41。
w.如实施方案a-v中任一项的酵母，其还包含编单羧酸/单羧酸盐转运蛋白多肽的异源基因。
x.如实施方案w的酵母，其中由酵母编码的单羧酸/单羧酸盐转运蛋白多肽具有氨基酸序列，所述氨基酸序列与以下氨基酸序列中的任一个具有至少50％、55％、65％、70％、75％、80％、85％、90％、95％、97％或99％的序列同一性：seqidno:22、seqidno:23、seqidno:24、seqidno:25或seqidno:26。
y.如实施方案a-x中任一项的酵母，其中所述酵母以足以在乙醇滴度为至少为75g/l时，将发酵液中的海藻糖含量降低至小于0.5g/l、1g/l、2g/l、3g/l、4g/l、5g/l、6g/l、7g/l、8g/l、9g/l或10g/l的量分泌海藻糖酶。
z.如实施方案a-y中任一项的酵母，其中所述酵母能够在细胞外分泌海藻糖酶。
aa.如实施方案a-z中任一项的酵母，其中由酵母编码的海藻糖酶多肽包含与seqidno：86具有至少76％、至少84％、至少92％或100％的序列同一性的序列。
bb.如实施方案a-aa中任一项的酵母，其中所述酵母能够以80g/l、90g/l、100g/l、110g/l、120g/l、125g/l、130g/l、135g/l或更高的滴度产生乙醇。
cc.一种用于生产乙醇的方法，所述方法包括：
使用经遗传修饰的酵母使培养基发酵，其中所述酵母包含异源海藻糖酶基因，其中发酵结束时的乙醇滴度至少为90g/l。
dd.如实施方案cc的方法，其中发酵温度在25至45℃、25至40℃、25至35℃、30至40℃或28至38℃的范围内。
ee.如实施方案cc-dd中任一项的方法，其中发酵结束时的乙醇滴度为至少80g/升、90g/升、100g/升、110g/升、120g/升、130g/升、135g/升、140g/升、145g/升、150g/升、155g/升或160g/升。
ff.如实施方案cc-ee中任一项的方法，其中所述酵母是如实施方案a-bb中任一项的酵母。
gg.如实施方案a-ff中任一项的酵母或方法，其中所述酵母还包含编码葡糖淀粉酶(ec3.2.1.3)多肽的异源基因。
hh.如实施方案gg的酵母或方法，其中由酵母编码的葡糖淀粉酶与以下多肽序列中的至少一个具有至少70％、75％、80％、85％、90％或95％的序列同一性：seqidno:16(sfga)、seqidno:17(roga)、seqidno:108(rmicga)或seqidno:109(rdelga)。
ii.如实施方案a-hh中任一项的酵母或方法，其中所述酵母包含编码甘油醛-3-磷酸脱氢酶(e.c.1.2.1.9)的重组核酸；以及减少的或消除的编码甘油-3-磷酸磷酸酶(e.c.3.1.3.21)的基因的表达。
jj.如实施方案a-hh中任一项的酵母或方法，其中所述酵母包含编码甘油醛-3-磷酸脱氢酶(gapn，e.c.1.2.1.9)的重组核酸。
kk.如实施方案a-jj中任一项的酵母或方法，其中所述编码甘油醛-3-磷酸脱氢酶(e.c.1.2.1.9)的重组核酸编码与seqidno：111(蜡样芽孢杆菌gapn)具有至少80％、85％、90％或95％的序列同一性的多肽。
本文引用的每个专利、专利申请或出版物的公开内容由此通过引用整体并入。虽然已参照具体的实施方案公开了本发明，但本发明的其它实施方案和变型可由本领域的其他技术人员在不脱离本发明的精神和范围的情况下设计。所附权利要求旨在被解释为包括所有此类实施方案和变型。
序列表
<110>嘉吉公司(cargill,incorporated)
彼得·豪尔特(jauert,peter)
格雷戈里·庞特(poynter,gregory)
布来恩·拉什(rush,brian)
<120>经遗传修饰的表达海藻糖酶的酵母及使用此类经遗传修饰的酵母的发酵方法
<130>n00525-us-psp
<150>62/501,288
<151>2017-05-04
<160>112
<170>patentin3.5版
<210>1
<211>1147
<212>prt
<213>乳酸克鲁维酵母(kluyveromyceslactis)
<400>1
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108510901095
metasnprogluasnvalaspileasppheargglyasnglnthr
110011051110
asptrparglysglyargpheilevalleuthrvalhisaspthr
111511201125
tyraspaspaspaspaspglulysglyalathrilelysgluphe
113011351140
alaleuphepro
1145
<210>2
<211>1071
<212>prt
<213>近平滑假丝酵母(candidaparapsilosis)
<400>2
metaspilegluasnasnglyiletyrmetgluasnserserargile
151015
lysglyileargargleuphelysaspasnlysthrtyrileileala
202530
metthrileilealaasnpheileleuphetyrserleuhisleuleu
354045
asnthrargpheserglualapheprophevalleuproargalaglu
505560
lystyrthrleugluglnthrseraspserasnleuglyasppheval
65707580
asnserphegluasnlysgluilepheserglnvalglnpheserglu
859095
hisalaphetyrasparglysmetasnvalvalglythrileglutyr
100105110
serlystyrasnglnpheglnlysglnprotyrvalalaasnglytyr
115120125
ileglyserargileproasnleuglyhisglyphethrtyraspgln
130135140
leuthrgluserproaspserlyslysaspaspleutyrasnglytrp
145150155160
proleupheasplysargpheserglyalapheilealaglyphetyr
165170175
aspleuglngluasnthrthrglyasnasnpheprogluleuleulys
180185190
asnglytyrgluservalileseralaileproglntrpthrthrleu
195200205
lysleualaileglnhisglyasplysargtyrthrleuaspproala
210215220
serproaspglyvalglyglnilethrasntyrvalglnasnmetser
225230235240
leuserthrglyilevalthrthrglnphethrtrpleugluserleu
245250255
aspvalargtyrthrvalleualahisargsergluileasnleugly
260265270
leuvallysvalaspilelysasnlysglyasnaspthrvalgluile
275280285
aspvalileaspgluleuasnpheaspthralaglnargcysserleu
290295300
asnleuileglyhisaspasnserglyiletyrvalserpheserpro
305310315320
aspasnleuasptyrileserglyalailetyrserthrleuhisleu
325330335
aspservallysarghisserlysasnlyslysthrasnglnthrser
340345350
serileargleuhisalaglnaspserthrglnpheglylysthrval
355360365
glyilevalserthraspleuaspproserlysphelysasnserarg
370375380
aspthrleugluphealalyslysvalserglnlyspheglutyrleu
385390395400
aspleuvalilelysserhisvalargalatrplysgluleumetgly
405410415
lysglnserthrilethrpheproasnaspproleuleuaspleugly
420425430
serlysalaservaltyrhisleuleualaasnthrargproaspala
435440445
glnglyleuthrglyalaleuglyvalalaglyleuserseraspser
450455460
tyrglyglymetvalphetrpaspthraspphetrpmetpheargala
465470475480
ileleualaleuaspprothrhisalalysserilevalasntyrarg
485490495
methisthrhisglnglnalaleulysasnthrproglnglytyrglu
500505510
glyalaalatyrprotrpthrserglyargpheglyasncysthrala
515520525
thrglyprocysleuasntyrglutyrhisileasnseralaileala
530535540
metalaalatrpgluleutyrileserglyalavalaspaspglntyr
545550555560
leugluserthrvaltyrproleuileseraspalaalalysphetyr
565570575
alaasptyrvalthrsertyrasngluthrileasplyspheilethr
580585590
serasnleuthraspproaspglutyralaasnhisvalaspasngly
595600605
alatyrthrasnalaglyilealaleuleumetasntrpileasnasp
610615620
ileglyaspileleuglyilelysmetprogluiletyrthraspile
625630635640
serglnlysmettyrleuprothralaaspasnserglnasnilethr
645650655
leuglutyrserglymetasnserservalglyilelysglnalaasp
660665670
valvalleumetthrtyrproleuglnasnserleuvalaspaspglu
675680685
glnalatyrileasnmetgluphetyrserglylysglnvalsertyr
690695700
glyproalametthrpheserilepheserilevalalaalaasnleu
705710715720
alathrserglycysalaserglnsertyrleuhislysalametgln
725730735
protyrleuargglyprophealaglnphesergluglnasnasnasp
740745750
asnphelysthrasnglyglythrhisproalatyrpropheleuthr
755760765
alahisglyglypheileglnalavalvalasnglyleuthrglyleu
770775780
arghissertyrthrileaspasnglylysileargargserleuphe
785790795800
leuaspproilealaleuprocysleuglyaspglyvalglntyrser
805810815
glyilehistyraspasnhisthrilesermetasnilethrglyser
820825830
thrphevalileargasngluglylysthrasnlysargalaasnasp
835840845
tyrileserilethrilealaaspargasnproglyargglythrtyr
850855860
lysleulysaspasngluglulysserphelysleutyrglnproser
865870875880
lysilephegluaspservalserglucysglyleualathrphetyr
885890895
asnilethrgluseralaproglyaspserserpheserileasnasp
900905910
glyaspasnthrthrargtrpglnvallystyrasnaspthrthrgly
915920925
lysvalleuileaspleuhisserilelysasnilethrglyvalval
930935940
pheasntrpalaasplysproprolyslysvallysleuserlystyr
945950955960
metglythrlysphelysaspvalthrasppheleualalysvalasp
965970975
pheglyasntyrvaltyrlysasntyrargphealaasnproglyglu
980985990
lysleuleuasnglnsergluvalphegluasnvaltyrseraspasp
99510001005
valthrileasnalapropheserserlysgluleuglugluval
101010151020
ilevalprovalarghisasnthrthrsertrpasnileasnleu
102510301035
glnglyargpheleuleuleugluvalaspglyilehisasnthr
104010451050
ileproilegluaspasptyrglyglyalalysleualagluval
105510601065
asnphephe
1070
<210>3
<211>1212
<212>prt
<213>光滑假丝酵母(candidaglabrata)
<400>3
metglyphelysasplysileleuphetrplysaspgluvalglntyr
151015
argthrleualavalalaaspglnvalalaasnargphephehisser
202530
phegluasnvaltyrlysglyaspgluservalgluaspalaaspser
354045
argprovalglyleuthrasngluthrleuserhisserseraspphe
505560
phevalleuproglugluargileserthrargvallysileargarg
65707580
glnasnileleuasnthrthrleuileleuglymetleuilealaleu
859095
valiletrpthralaileleuserthrasnsertyrpheserserser
100105110
leualaseralaserproleupheasnlysgluglyargvalvalarg
115120125
prometarggluserasnleuglyleuhisalaaspproglnthrarg
130135140
lysserserlysthrleutyraspleuleuserasppheaspasnala
145150155160
phetyraspaspgluasnmetileleuglyserleualapheglyglu
165170175
asnthrtyrserargglnprotyrvalalaasnglytyrileglyser
180185190
argileproasnileglypheglytyralaleuaspthrleuasnleu
195200205
tyralaaspalaproglyalaleuasnasnglytrpproleuargasn
210215220
argargphealaglyserphevalseraspphetyrserleuglnala
225230235240
lysleuasnserthrasnpheprogluleuaspglulysglytyrthr
245250255
thrvalileserserileproglutrpthraspleuglnphethrval
260265270
aspleuasnglythrasntrppheasnproglnservalleuileasp
275280285
aspvalileasntyrasnglnasnleusermetlysaspglyileval
290295300
serthrasnmetasptrpleuasnglymetileasnilelysserglu
305310315320
valtrpalahisarglysilehisserleuglyilethrargleuglu
325330335
ileserleuasnleuaspalaleuproaspgluphethrgluleupro
340345350
valthriletyraspileileasppheasnthrserhisargthrthr
355360365
leutyrglulysglyglnaspgluaspasnlysalailetyrmetile
370375380
valasnprogluasnvalprotyrserasnalavalvaltyrserthr
385390395400
cysthrilelysglythrgluasnasnpheserprotyrasnphethr
405410415
seraspaspargilealaargasntyrmetthrasnleuthrgluglu
420425430
asnprolysvalvaliletyrlystyrthrservalvalserserglu
435440445
tyrasnasnaspgluproasnproasnvalasnleulysphealaser
450455460
asnilealaasnthralalysglyasntyrlysserleuleuserasn
465470475480
hislysargalatrptyraspleutyrasnaspalapheilegluile
485490495
proseraspserleuleuglumetthralaargserserleuphehis
500505510
leuleualaasnthrargglntyrasnvalserthrthrargglyleu
515520525
provalglyvalglyglyleuserseraspsertyrglyglymetval
530535540
phetrpaspalaaspvaltrpmetalaproalaleuleuprophephe
545550555560
proasnilealametasnmetasnasntyrargasnalathrhisgln
565570575
glnalailegluasnalalysglntyrasntyrproglyalavaltyr
580585590
protrpthrserglyargtyralaasncysthrserthrglyprocys
595600605
ileasptyrglutyrhisileasnvalaspilealaleualaserphe
610615620
seriletyrmetasnglyalagluglyalaaspgluasptyrleuarg
625630635640
phethrthrtrpprometvallysaspalaalavalphephelysala
645650655
tyrvallystyrasngluthrleuglyglutyrgluthrtyrasnleu
660665670
thraspproaspgluphealaasnhisvalasnasnglyalaphethr
675680685
asnalaglyilelysthrleuleulystrpalathraspileglythr
690695700
hisleuglyglugluvalaspprolystrpmetgluilealaaspasn
705710715720
ilehisileproargseraspserasnilethrleuglutyrsergly
725730735
metasnserservalgluilelysglnalaaspvalthrleumetval
740745750
tyrproleuglytyrileasnaspgluserileleuasnasnalaile
755760765
lysaspleutyrtyrtyrsergluargglnseralaserglyproala
770775780
metthrtyrprovalphevalalaalaalaalaserleuleuasnhis
785790795800
glyserserserglnsertyrleutyrlysservalleuprotyrleu
805810815
argserprophealaglnphesergluglnseraspaspasnpheleu
820825830
thrasnglyleuthrglnproalaphepropheleuthralaasngly
835840845
glypheleuglnserileleupheglyleuthrglyleuargtyrser
850855860
tyrgluvalthrproargthrlyslysileserargleuleulysphe
865870875880
aspprovallysleuproleuleuproglyglyilealaileargasn
885890895
phelystyrmetglyglnvalleuaspileileileaspaspasnasn
900905910
glythrilealahislysglyglyasplysproileargilelysval
915920925
proasnargaspileleuhisaspargasnilethrseralaleutyr
930935940
serlysargaspaspaspleuseralathraspasptyrtyrglythr
945950955960
tyrphethrleutyrproasnglugluleuvalileproleutyrasp
965970975
thrlysleuasnileaspglyasnilealagluserlysglnilethr
980985990
asnleuthralaglyvalproglyaspvalglypheseralaleuasp
99510001005
glyasnasntyrthrhistrpglnpropheasplysseraspasn
101010151020
alalysleuleuileaspleuglypheasnserthrhisvalile
102510301035
lyslysglyileileleutrpglyglnargproalalysasnile
104010451050
serleuservalleuprohissergluargilegluglnleuphe
105510601065
alaasnilethraspleuleugluthrserserilethrlysgly
107010751080
glyserleuleuasnglnmetleuglyglnthrglnserasnval
108510901095
thralagluileaspaspaspileleualaleuleuasntrplys
110011051110
glyaspaspleuaspglnleuileprotyrleuproaspmethis
111511201125
leuleuglnglulyspheileproileleulysasptyrproile
113011351140
lysproasnglnargtyrtyrlysgluileileaspaspaspile
114511501155
ilelysleuleuproserasnthrthrgluphethrileasptyr
116011651170
asnserileproglyglyglulysargalaargtyrvalvalleu
117511801185
thrvalhisglythrtyraspaspaspaspaspleulysglyala
119011951200
thrilelysgluilevalleuglnglu
12051210
<210>4
<211>19
<212>prt
<213>酿酒酵母(saccharomycescerevisiae)
<400>4
metlyspheileserthrpheleuthrpheileleualaalavalser
151015
valthrala
<210>5
<211>683
<212>dna
<213>酿酒酵母(saccharomycescerevisiae)
<400>5
atctcccgagtttatcattatcaatactgccatttcaaagaatacgtaaataattaatag60
tagtgattttcctaactttatttagtcaaaaaattggccttttaattctgctgtaacccg120
tacatgcccaaaatagggggcgggttacacagaatatataacatcataggtgtctgggtg180
aacagtttattcctggcatccactaaatataatggagcccgctttttttaagctggcatc240
cagaaaaaaaaagaatcccagcaccaaaatattgttttcttcaccaaccatcagttcata300
ggtccattctcttagcgcaactacacagaacaggggcacaaacaggcaaaaaacgggcac360
aacctcaatggagtgatgcaacctgcttggagtaaatgatgacacaaggcaattgaccta420
cgcatgtatctatctcattttcttacaccttctattaccttctgctctctctgatttgga480
aaaagctgaaaaaaaaggttgaaaccagttccctgaaattattcccctatttgactaata540
agtatataaagacggtaggtattgattgtaattctgtaaatctatttcttaaacttctta600
aattctacttttatagttagtcttttttttagttttaaaacactaagaacttagtttcga660
ataaacacacataaacaaacaaa683
<210>6
<211>5745
<212>dna
<213>人工序列(artificialsequence)
<220>
<223>由多个单独的酿酒酵母序列共同组成的序列
<400>6
tcgagatctcccgagtttatcattatcaatactgccatttcaaagaatacgtaaataatt60
aatagtagtgattttcctaactttatttagtcaaaaaattggccttttaattctgctgta120
acccgtacatgcccaaaatagggggcgggttacacagaatatataacatcataggtgtct180
gggtgaacagtttattcctggcatccactaaatataatggagcccgctttttttaagctg240
gcatccagaaaaaaaaagaatcccagcaccaaaatattgttttcttcaccaaccatcagt300
tcataggtccattctcttagcgcaactacacagaacaggggcacaaacaggcaaaaaacg360
ggcacaacctcaatggagtgatgcaacctgcttggagtaaatgatgacacaaggcaattg420
acctacgcatgtatctatctcattttcttacaccttctattaccttctgctctctctgat480
ttggaaaaagctgaaaaaaaaggttgaaaccagttccctgaaattattcccctatttgac540
taataagtatataaagacggtaggtattgattgtaattctgtaaatctatttcttaaact600
tcttaaattctacttttatagttagtcttttttttagttttaaaacactaagaacttagt660
ttcgaataaacacacataaacaaacaaatctagattaattaaacaggccccttttccttt720
gtcgatatcatgtaattagttatgtcacgcttacattcacgccctcctcccacatccgct780
ctaaccgaaaaggaaggagttagacaacctgaagtctaggtccctatttatttttttata840
gttatgttagtattaagaacgttatttatatttcaaatttttcttttttttctgtacaaa900
cgcgtgtacgcatgtaacgggcagacgcggccgccaccgcggtggagctccaattcgccc960
tatagtgagtcgtattacaattcactggccgtcgttttacaacgtcgtgactgggaaaac1020
cctggcgttacccaacttaatcgccttgcagcacatccccccttcgccagctggcgtaat1080
agcgaagaggcccgcaccgatcgcccttcccaacagttgcgcagcctgaatggcgaatgg1140
cgcgacgcgccctgtagcggcgcattaagcgcggcgggtgtggtggttacgcgcagcgtg1200
accgctacacttgccagcgccctagcgcccgctcctttcgctttcttcccttcctttctc1260
gccacgttcgccggctttccccgtcaagctctaaatcgggggctccctttagggttccga1320
tttagtgctttacggcacctcgaccccaaaaaacttgattagggtgatggttcacgtagt1380
gggccatcgccctgatagacggtttttcgccctttgacgttggagtccacgttctttaat1440
agtggactcttgttccaaactggaacaacactcaaccctatctcggtctattcttttgat1500
ttataagggattttgccgatttcggcctattggttaaaaaatgagctgatttaacaaaaa1560
tttaacgcgaattttaacaaaatattaacgtttacaatttcctgatgcggtattttctcc1620
ttacgcatctgtgcggtatttcacaccgcagggtaataactgatataattaaattgaagc1680
tctaatttgtgagtttagtatacatgcatttacttataatacagttttttagttttgctg1740
gccgcatcttctcaaatatgcttcccagcctgcttttctgtaacgttcaccctctacctt1800
agcatcccttccctttgcaaatagtcctcttccaacaataataatgtcagatcctgtaga1860
gaccacatcatccacggttctatactgttgacccaatgcgtctcccttgtcatctaaacc1920
cacaccgggtgtcataatcaaccaatcgtaaccttcatctcttccacccatgtctctttg1980
agcaataaagccgataacaaaatctttgtcgctcttcgcaatgtcaacagtacccttagt2040
atattctccagtagatagggagcccttgcatgacaattctgctaacatcaaaaggcctct2100
aggttcctttgttacttcttctgccgcctgcttcaaaccgctaacaatacctgggcccac2160
cacaccgtgtgcattcgtaatgtctgcccattctgctattctgtatacacccgcagagta2220
ctgcaatttgactgtattaccaatgtcagcaaattttctgtcttcgaagagtaaaaaatt2280
gtacttggcggataatgcctttagcggcttaactgtgccctccatggaaaaatcagtcaa2340
gatatccacatgtgtttttagtaaacaaattttgggacctaatgcttcaactaactccag2400
taattccttggtggtacgaacatccaatgaagcacacaagtttgtttgcttttcgtgcat2460
gatattaaatagcttggcagcaacaggactaggatgagtagcagcacgttccttatatgt2520
agctttcgacatgatttatcttcgtttcctgcaggtttttgttctgtgcagttgggttaa2580
gaatactgggcaatttcatgtttcttcaacactacatatgcgtatatataccaatctaag2640
tctgtgctccttccttcgttcttccttctgttcggagattaccgaatcaaaaaaatttca2700
aagaaaccgaaatcaaaaaaaagaataaaaaaaaaatgatgaattgaattgaaaagcgtg2760
gtgcactctcagtacaatctgctctgatgccgcatagttaagccagccccgacacccgcc2820
aacacccgctgacgcgccctgacgggcttgtctgctcccggcatccgcttacagacaagc2880
tgtgaccgtctccgggagctgcatgtgtcagaggttttcaccgtcatcaccgaaacgcgc2940
gagacgaaagggcctcgtgatacgcctatttttataggttaatgtcatgataataatggt3000
ttcttaggacggatcgcttgcctgtaacttacacgcgcctcgtatcttttaatgatggaa3060
taatttgggaatttactctgtgtttatttatttttatgttttgtatttggattttagaaa3120
gtaaataaagaaggtagaagagttacggaatgaagaaaaaaaaataaacaaaggtttaaa3180
aaatttcaacaaaaagcgtactttacatatatatttattagacaagaaaagcagattaaa3240
tagatatacattcgattaacgataagtaaaatgtaaaatcacaggattttcgtgtgtggt3300
cttctacacagacaagatgaaacaattcggcattaatacctgagagcaggaagagcaaga3360
taaaaggtagtatttgttggcgatccccctagagtcttttacatcttcggaaaacaaaaa3420
ctattttttctttaatttctttttttactttctatttttaatttatatatttatattaaa3480
aaatttaaattataattatttttatagcacgtgatgaaaaggacccaggtggcacttttc3540
ggggaaatgtgcgcggaacccctatttgtttatttttctaaatacattcaaatatgtatc3600
cgctcatgagacaataaccctgataaatgcttcaataatattgaaaaaggaagagtatga3660
gtattcaacatttccgtgtcgcccttattcccttttttgcggcattttgccttcctgttt3720
ttgctcacccagaaacgctggtgaaagtaaaagatgctgaagatcagttgggtgcacgag3780
tgggttacatcgaactggatctcaacagcggtaagatccttgagagttttcgccccgaag3840
aacgttttccaatgatgagcacttttaaagttctgctatgtggcgcggtattatcccgta3900
ttgacgccgggcaagagcaactcggtcgccgcatacactattctcagaatgacttggttg3960
agtactcaccagtcacagaaaagcatcttacggatggcatgacagtaagagaattatgca4020
gtgctgccataaccatgagtgataacactgcggccaacttacttctgacaacgatcggag4080
gaccgaaggagctaaccgctttttttcacaacatgggggatcatgtaactcgccttgatc4140
gttgggaaccggagctgaatgaagccataccaaacgacgagcgtgacaccacgatgcctg4200
tagcaatggcaacaacgttgcgcaaactattaactggcgaactacttactctagcttccc4260
ggcaacaattaatagactggatggaggcggataaagttgcaggaccacttctgcgctcgg4320
cccttccggctggctggtttattgctgataaatctggagccggtgagcgtgggtctcgcg4380
gtatcattgcagcactggggccagatggtaagccctcccgtatcgtagttatctacacga4440
cgggcagtcaggcaactatggatgaacgaaatagacagatcgctgagataggtgcctcac4500
tgattaagcattggtaactgtcagaccaagtttactcatatatactttagattgatttaa4560
aacttcatttttaatttaaaaggatctaggtgaagatcctttttgataatctcatgacca4620
aaatcccttaacgtgagttttcgttccactgagcgtcagaccccgtagaaaagatcaaag4680
gatcttcttgagatcctttttttctgcgcgtaatctgctgcttgcaaacaaaaaaaccac4740
cgctaccagcggtggtttgtttgccggatcaagagctaccaactctttttccgaaggtaa4800
ctggcttcagcagagcgcagataccaaatactgtccttctagtgtagccgtagttaggcc4860
accacttcaagaactctgtagcaccgcctacatacctcgctctgctaatcctgttaccag4920
tggctgctgccagtggcgataagtcgtgtcttaccgggttggactcaagacgatagttac4980
cggataaggcgcagcggtcgggctgaacggggggttcgtgcacacagcccagcttggagc5040
gaacgacctacaccgaactgagatacctacagcgtgagcattgagaaagcgccacgcttc5100
ccgaagggagaaaggcggacaggtatccggtaagcggcagggtcggaacaggagagcgca5160
cgagggagcttccaggggggaacgcctggtatctttatagtcctgtcgggtttcgccacc5220
tctgacttgagcgtcgatttttgtgatgctcgtcaggggggccgagcctatggaaaaacg5280
ccagcaacgcggcctttttacggttcctggccttttgctggccttttgctcacatgttct5340
ttcctgcgttatcccctgattctgtggataaccgtattaccgcctttgagtgagctgata5400
ccgctcgccgcagccgaacgaccgagcgcagcgagtcagtgagcgaggaagcggaagagc5460
gcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacg5520
acaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttacctca5580
ctcattaggcaccccaggctttacactttatgcttccggctcctatgttgtgtggaattg5640
tgagcggataacaatttcacacaggaaacagctatgaccatgattacgccaagctcggaa5700
ttaaccctcactaaagggaacaaaagctgggtaccgggccccccc5745
<210>7
<211>9181
<212>dna
<213>人工序列(artificialsequence)
<220>
<223>由多种生物体乳酸克鲁维酵母和酿酒酵母共同组成的序列
<400>7
gtttatttttctaaatacattcaaatatgtatccgctcatgagacaataaccctgataaa60
tgcttcaataatattgaaaaaggaagagtatgagtattcaacatttccgtgtcgccctta120
ttcccttttttgcggcattttgccttcctgtttttgctcacccagaaacgctggtgaaag180
taaaagatgctgaagatcagttgggtgcacgagtgggttacatcgaactggatctcaaca240
gcggtaagatccttgagagttttcgccccgaagaacgttttccaatgatgagcactttta300
aagttctgctatgtggcgcggtattatcccgtattgacgccgggcaagagcaactcggtc360
gccgcatacactattctcagaatgacttggttgagtactcaccagtcacagaaaagcatc420
ttacggatggcatgacagtaagagaattatgcagtgctgccataaccatgagtgataaca480
ctgcggccaacttacttctgacaacgatcggaggaccgaaggagctaaccgctttttttc540
acaacatgggggatcatgtaactcgccttgatcgttgggaaccggagctgaatgaagcca600
taccaaacgacgagcgtgacaccacgatgcctgtagcaatggcaacaacgttgcgcaaac660
tattaactggcgaactacttactctagcttcccggcaacaattaatagactggatggagg720
cggataaagttgcaggaccacttctgcgctcggcccttccggctggctggtttattgctg780
ataaatctggagccggtgagcgtgggtctcgcggtatcattgcagcactggggccagatg840
gtaagccctcccgtatcgtagttatctacacgacgggcagtcaggcaactatggatgaac900
gaaatagacagatcgctgagataggtgcctcactgattaagcattggtaactgtcagacc960
aagtttactcatatatactttagattgatttaaaacttcatttttaatttaaaaggatct1020
aggtgaagatcctttttgataatctcatgaccaaaatcccttaacgtgagttttcgttcc1080
actgagcgtcagaccccgtagaaaagatcaaaggatcttcttgagatcctttttttctgc1140
gcgtaatctgctgcttgcaaacaaaaaaaccaccgctaccagcggtggtttgtttgccgg1200
atcaagagctaccaactctttttccgaaggtaactggcttcagcagagcgcagataccaa1260
atactgtccttctagtgtagccgtagttaggccaccacttcaagaactctgtagcaccgc1320
ctacatacctcgctctgctaatcctgttaccagtggctgctgccagtggcgataagtcgt1380
gtcttaccgggttggactcaagacgatagttaccggataaggcgcagcggtcgggctgaa1440
cggggggttcgtgcacacagcccagcttggagcgaacgacctacaccgaactgagatacc1500
tacagcgtgagcattgagaaagcgccacgcttcccgaagggagaaaggcggacaggtatc1560
cggtaagcggcagggtcggaacaggagagcgcacgagggagcttccaggggggaacgcct1620
ggtatctttatagtcctgtcgggtttcgccacctctgacttgagcgtcgatttttgtgat1680
gctcgtcaggggggccgagcctatggaaaaacgccagcaacgcggcctttttacggttcc1740
tggccttttgctggccttttgctcacatgttctttcctgcgttatcccctgattctgtgg1800
ataaccgtattaccgcctttgagtgagctgataccgctcgccgcagccgaacgaccgagc1860
gcagcgagtcagtgagcgaggaagcggaagagcgcccaatacgcaaaccgcctctccccg1920
cgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggca1980
gtgagcgcaacgcaattaatgtgagttacctcactcattaggcaccccaggctttacact2040
ttatgcttccggctcctatgttgtgtggaattgtgagcggataacaatttcacacaggaa2100
acagctatgaccatgattacgccaagctcggaattaaccctcactaaagggaacaaaagc2160
tgggtaccgggccccccctcgagatctcccgagtttatcattatcaatactgccatttca2220
aagaatacgtaaataattaatagtagtgattttcctaactttatttagtcaaaaaattgg2280
ccttttaattctgctgtaacccgtacatgcccaaaatagggggcgggttacacagaatat2340
ataacatcataggtgtctgggtgaacagtttattcctggcatccactaaatataatggag2400
cccgctttttttaagctggcatccagaaaaaaaaagaatcccagcaccaaaatattgttt2460
tcttcaccaaccatcagttcataggtccattctcttagcgcaactacacagaacaggggc2520
acaaacaggcaaaaaacgggcacaacctcaatggagtgatgcaacctgcttggagtaaat2580
gatgacacaaggcaattgacctacgcatgtatctatctcattttcttacaccttctatta2640
ccttctgctctctctgatttggaaaaagctgaaaaaaaaggttgaaaccagttccctgaa2700
attattcccctatttgactaataagtatataaagacggtaggtattgattgtaattctgt2760
aaatctatttcttaaacttcttaaattctacttttatagttagtcttttttttagtttta2820
aaacactaagaacttagtttcgaataaacacacataaaaacaaatctagaatgaagttca2880
tttcaactttcttgacttttatcttggctgccgttagtgttactgctaccaaacctttac2940
cattacaacatactcattttggctcttttgtctgcgctgaggataccttattctgccctg3000
aatctcagagaaaagcctctgaaaagatgtatagtttattgaaggatcacgaaaacacat3060
tctacgatgaggaacagcaaatcctgggaaacttattgttatcagaaaatacattctcaa3120
gacaaccatatgttgcaaacggttacattggatccagaatccctaaccttgggttcggat3180
acgcacttgataccattaacgtttgggtaaatgactcctctataccaggcgcccttgata3240
atggttggccattgagaaaccagagatttgctggggcattcatttcagacttctactgtc3300
tacaagagaagctcaactcaactaacttccctgaattggacgatgatggttactcaactg3360
ttatatcaacaataccacaatggacagatctctcaattttaaagcacactactacaggtc3420
aaatcgagtacattaatcctacagatgttaagttagataagattacaaactatatgcaaa3480
acttatcacttcaagatggaattgtaacaactacatttgtttacgacaaacaactattgg3540
ttactacaaaggtagttgcacatagaaagatataccctctcggagtcgttaccttagaac3600
tatctttgttcgacaacaattctgatactagtgctgacaacgaaaacgaatacgtcgaat3660
tagagatctgtgattcactaaacttctctacatcccacagaaccgtacttaatgattacg3720
gttacgatcaaaacaacgagggtattttcatggtggtggagccagaaaacgtcccatatt3780
ctaatgcatcccttttttcatattttgatatcccaagtagagatacattaacactttcta3840
agtactccgattcaatcagtcaatgtacaacacaaatcttaaaggcaaattcaacttttg3900
tagctcacaagtacatcggtataattagctctgagtacgataacaaacaacctgatgata3960
actctaatggaacttctaatgaagcgcttaaaatgtctaacctggaacgtgcaaccagta4020
ttgtgcttgataacaaaggtaattacgattctctgattcaatcacataaaaatgcttgga4080
agagaatttacaaagatgctagcattgaaataccatccgatggtttgctggagatgacag4140
caaaaagttctatttaccatttactagcaaatacaagatctcataacgtgtctgaggaca4200
gaggtttgcctataggcgtgtcaggattatcttctgattcttacgggggtatggttttct4260
gggattctgacttgtggattctaccagcactcctgccattcttcccaaacgctgcaagac4320
agatcaataactacagaaatgcaagccttcaccaggccaagctaaacgcagaaaagtacg4380
gctacgatggcgcgctatacccatggacatctggcagatacgcgaactgtacttcaactg4440
gtccatgtgtcgactacgaatatcatatcaacgtggacatcgctttgtcatctttcgcca4500
tctacatgaatggtgatgaagatgacgaacgttccgaagagtacttgcgttacacaacat4560
ggccattcgtagaaaacgcagcaaagatgtttgctcaatacgttaagtggaatgacacaa4620
tgcaacaatacaccactcataacttgactgatccagatgaatatgcaaacttcgttgaca4680
acgctgcatttactaatgctggtatacaatctgtgatggtctgggctcatgatatcgcca4740
gacacttgggcatcgatccagatccacagtggttggagatagccgacaacatccatattc4800
caatttccgagacaaacatcacattggaatactcaggtatgaactcctctgtcgaaatca4860
agcaagctgatgtggtattgatgatttacccattatcttacataacagatcaaagcatct4920
tgaacaacgcaattaaaaacttgtactactattctgaacgtcaaagtgcatcagggcctg4980
ctatgacatacccagtatttgtcgccggagcggcatctcttcttaactacggttcatctt5040
cacaatcctatctatacaagtcagttgtcccatacttacgtagtccttttgctcaattct5100
cagaacaaagtgacgacaacttcttgaccaatggtctaacacagccagcgttcccatttc5160
taacagctaacggtggtttcttgcagtctattctattcggtttgactgggctcagataca5220
gttacgaagttgatgaagatacagggaaaatccatagattactgaagttcaatccaattc5280
agttgcctctcctacctggaggtattagaattaacaactttaagtacatgggtcaggtat5340
tagatgttttgatcacagacaccgagggtatcatcatccacaaaaacggtactaaggaaa5400
tcagaatcaaagttccagatagaactctaattcctgatgtagatgtcaagtactcagaag5460
agactgatcctattaagcaaattttgcatggtagaagatctgttccaaccggtaaaaact5520
acttcactatacaacctggtgatgtgttcaagacaccactttacatccctaaaaaaaact5580
tggaaggcaatttggtcgaggctaagcaaattaccaatttgactgccggcgtcccaggtg5640
acgttgctgtctctgtaatagatggaaacaactttacacactggcaaccagcatacaaaa5700
accttccagccagattgttaatcgatatgggcaataattttacccaagagattaagtccg5760
gcaagatcatatggggctcaagaccagcaaaaacattctccctgtcaattttgccacaga5820
cagaagaggttttcaaaaatcttacacatattttatctaacgttaatcaatattgtaaca5880
agacaggtgatgagtgcgtcagagatttagagagaacagagaaagggttcgatgccgcaa5940
tagaagatgttttcgactggtacggtattgacccagacagcattatctcaacacatcctg6000
aattaaaagatatgaaaacaaagttcgtcaagatcctagatcactacaaagtcactccat6060
ccgaaccttatccatggagagtttacaacgagtctcaaatcgtgctgcttccaggcaatg6120
aaacagatttcgatattgattactctaaagttgctgaaatgaaccctgaaaacgttgata6180
ttgatttcagagggaaccagacagattggagaaagggaagatttatcgttctcacagtgc6240
atgatacttacgacgacgatgatgacgaaaagggtgccactatcaaggaatttgcattat6300
tcccttgattaattaaacaggccccttttcctttgtcgatatcatgtaattagttatgtc6360
acgcttacattcacgccctcctcccacatccgctctaaccgaaaaggaaggagttagaca6420
acctgaagtctaggtccctatttatttttttatagttatgttagtattaagaacgttatt6480
tatatttcaaatttttcttttttttctgtacaaacgcgtgtacgcatgtaacgggcagac6540
gcggccgccaccgcggtggagctccaattcgccctatagtgagtcgtattacaattcact6600
ggccgtcgttttacaacgtcgtgactgggaaaaccctggcgttacccaacttaatcgcct6660
tgcagcacatccccccttcgccagctggcgtaatagcgaagaggcccgcaccgatcgccc6720
ttcccaacagttgcgcagcctgaatggcgaatggcgcgacgcgccctgtagcggcgcatt6780
aagcgcggcgggtgtggtggttacgcgcagcgtgaccgctacacttgccagcgccctagc6840
gcccgctcctttcgctttcttcccttcctttctcgccacgttcgccggctttccccgtca6900
agctctaaatcgggggctccctttagggttccgatttagtgctttacggcacctcgaccc6960
caaaaaacttgattagggtgatggttcacgtagtgggccatcgccctgatagacggtttt7020
tcgccctttgacgttggagtccacgttctttaatagtggactcttgttccaaactggaac7080
aacactcaaccctatctcggtctattcttttgatttataagggattttgccgatttcggc7140
ctattggttaaaaaatgagctgatttaacaaaaatttaacgcgaattttaacaaaatatt7200
aacgtttacaatttcctgatgcggtattttctccttacgcatctgtgcggtatttcacac7260
cgcagggtaataactgatataattaaattgaagctctaatttgtgagtttagtatacatg7320
catttacttataatacagttttttagttttgctggccgcatcttctcaaatatgcttccc7380
agcctgcttttctgtaacgttcaccctctaccttagcatcccttccctttgcaaatagtc7440
ctcttccaacaataataatgtcagatcctgtagagaccacatcatccacggttctatact7500
gttgacccaatgcgtctcccttgtcatctaaacccacaccgggtgtcataatcaaccaat7560
cgtaaccttcatctcttccacccatgtctctttgagcaataaagccgataacaaaatctt7620
tgtcgctcttcgcaatgtcaacagtacccttagtatattctccagtagatagggagccct7680
tgcatgacaattctgctaacatcaaaaggcctctaggttcctttgttacttcttctgccg7740
cctgcttcaaaccgctaacaatacctgggcccaccacaccgtgtgcattcgtaatgtctg7800
cccattctgctattctgtatacacccgcagagtactgcaatttgactgtattaccaatgt7860
cagcaaattttctgtcttcgaagagtaaaaaattgtacttggcggataatgcctttagcg7920
gcttaactgtgccctccatggaaaaatcagtcaagatatccacatgtgtttttagtaaac7980
aaattttgggacctaatgcttcaactaactccagtaattccttggtggtacgaacatcca8040
atgaagcacacaagtttgtttgcttttcgtgcatgatattaaatagcttggcagcaacag8100
gactaggatgagtagcagcacgttccttatatgtagctttcgacatgatttatcttcgtt8160
tcctgcaggtttttgttctgtgcagttgggttaagaatactgggcaatttcatgtttctt8220
caacactacatatgcgtatatataccaatctaagtctgtgctccttccttcgttcttcct8280
tctgttcggagattaccgaatcaaaaaaatttcaaagaaaccgaaatcaaaaaaaagaat8340
aaaaaaaaaatgatgaattgaattgaaaagcgtggtgcactctcagtacaatctgctctg8400
atgccgcatagttaagccagccccgacacccgccaacacccgctgacgcgccctgacggg8460
cttgtctgctcccggcatccgcttacagacaagctgtgaccgtctccgggagctgcatgt8520
gtcagaggttttcaccgtcatcaccgaaacgcgcgagacgaaagggcctcgtgatacgcc8580
tatttttataggttaatgtcatgataataatggtttcttaggacggatcgcttgcctgta8640
acttacacgcgcctcgtatcttttaatgatggaataatttgggaatttactctgtgttta8700
tttatttttatgttttgtatttggattttagaaagtaaataaagaaggtagaagagttac8760
ggaatgaagaaaaaaaaataaacaaaggtttaaaaaatttcaacaaaaagcgtactttac8820
atatatatttattagacaagaaaagcagattaaatagatatacattcgattaacgataag8880
taaaatgtaaaatcacaggattttcgtgtgtggtcttctacacagacaagatgaaacaat8940
tcggcattaatacctgagagcaggaagagcaagataaaaggtagtatttgttggcgatcc9000
ccctagagtcttttacatcttcggaaaacaaaaactattttttctttaatttcttttttt9060
actttctatttttaatttatatatttatattaaaaaatttaaattataattatttttata9120
gcacgtgatgaaaaggacccaggtggcacttttcggggaaatgtgcgcggaacccctatt9180
t9181
<210>8
<211>1145
<212>prt
<213>人工序列(artificialsequence)
<220>
<223>由多种生物体乳酸克鲁维酵母和酿酒酵母共同组成的序列
<400>8
metlyspheileserthrpheleuthrpheileleualaalavalser
151015
valthralathrlysproleuproleuglnhisthrhispheglyser
202530
phevalcysalagluaspthrleuphecysprogluserglnarglys
354045
alaserglulysmettyrserleuleulysasphisgluasnthrphe
505560
tyraspglugluglnglnileleuglyasnleuleuleusergluasn
65707580
thrpheserargglnprotyrvalalaasnglytyrileglyserarg
859095
ileproasnleuglypheglytyralaleuaspthrileasnvaltrp
100105110
valasnaspserserileproglyalaleuaspasnglytrpproleu
115120125
argasnglnargphealaglyalapheileseraspphetyrcysleu
130135140
glnglulysleuasnserthrasnpheprogluleuaspaspaspgly
145150155160
tyrserthrvalileserthrileproglntrpthraspleuserile
165170175
leulyshisthrthrthrglyglnileglutyrileasnprothrasp
180185190
vallysleuasplysilethrasntyrmetglnasnleuserleugln
195200205
aspglyilevalthrthrthrphevaltyrasplysglnleuleuval
210215220
thrthrlysvalvalalahisarglysiletyrproleuglyvalval
225230235240
thrleugluleuserleupheaspasnasnseraspthrseralaasp
245250255
asngluasnglutyrvalgluleugluilecysaspserleuasnphe
260265270
serthrserhisargthrvalleuasnasptyrglytyraspglnasn
275280285
asngluglyilephemetvalvalgluprogluasnvalprotyrser
290295300
asnalaserleuphesertyrpheaspileproserargaspthrleu
305310315320
thrleuserlystyrseraspserileserglncysthrthrglnile
325330335
leulysalaasnserthrphevalalahislystyrileglyileile
340345350
serserglutyraspasnlysglnproaspaspasnserasnglythr
355360365
serasnglualaleulysmetserasnleugluargalathrserile
370375380
valleuaspasnlysglyasntyraspserleuileglnserhislys
385390395400
asnalatrplysargiletyrlysaspalaserilegluileproser
405410415
aspglyleuleuglumetthralalysserseriletyrhisleuleu
420425430
alaasnthrargserhisasnvalsergluaspargglyleuproile
435440445
glyvalserglyleuserseraspsertyrglyglymetvalphetrp
450455460
aspseraspleutrpileleuproalaleuleuprophepheproasn
465470475480
alaalaargglnileasnasntyrargasnalaserleuhisglnala
485490495
lysleuasnalaglulystyrglytyraspglyalaleutyrprotrp
500505510
thrserglyargtyralaasncysthrserthrglyprocysvalasp
515520525
tyrglutyrhisileasnvalaspilealaleuserserphealaile
530535540
tyrmetasnglyaspgluaspaspgluargsergluglutyrleuarg
545550555560
tyrthrthrtrpprophevalgluasnalaalalysmetphealagln
565570575
tyrvallystrpasnaspthrmetglnglntyrthrthrhisasnleu
580585590
thraspproaspglutyralaasnphevalaspasnalaalaphethr
595600605
asnalaglyileglnservalmetvaltrpalahisaspilealaarg
610615620
hisleuglyileaspproaspproglntrpleugluilealaaspasn
625630635640
ilehisileproilesergluthrasnilethrleuglutyrsergly
645650655
metasnserservalgluilelysglnalaaspvalvalleumetile
660665670
tyrproleusertyrilethraspglnserileleuasnasnalaile
675680685
lysasnleutyrtyrtyrsergluargglnseralaserglyproala
690695700
metthrtyrprovalphevalalaglyalaalaserleuleuasntyr
705710715720
glyserserserglnsertyrleutyrlysservalvalprotyrleu
725730735
argserprophealaglnphesergluglnseraspaspasnpheleu
740745750
thrasnglyleuthrglnproalaphepropheleuthralaasngly
755760765
glypheleuglnserileleupheglyleuthrglyleuargtyrser
770775780
tyrgluvalaspgluaspthrglylysilehisargleuleulysphe
785790795800
asnproileglnleuproleuleuproglyglyileargileasnasn
805810815
phelystyrmetglyglnvalleuaspvalleuilethraspthrglu
820825830
glyileileilehislysasnglythrlysgluileargilelysval
835840845
proaspargthrleuileproaspvalaspvallystyrsergluglu
850855860
thraspproilelysglnileleuhisglyargargservalprothr
865870875880
glylysasntyrphethrileglnproglyaspvalphelysthrpro
885890895
leutyrileprolyslysasnleugluglyasnleuvalglualalys
900905910
glnilethrasnleuthralaglyvalproglyaspvalalavalser
915920925
valileaspglyasnasnphethrhistrpglnproalatyrlysasn
930935940
leuproalaargleuleuileaspmetglyasnasnphethrglnglu
945950955960
ilelysserglylysileiletrpglyserargproalalysthrphe
965970975
serleuserileleuproglnthrglugluvalphelysasnleuthr
980985990
hisileleuserasnvalasnglntyrcysasnlysthrglyaspglu
99510001005
cysvalargaspleugluargthrglulysglypheaspalaala
101010151020
ilegluaspvalpheasptrptyrglyileaspproaspserile
102510301035
ileserthrhisprogluleulysaspmetlysthrlyspheval
104010451050
lysileleuasphistyrlysvalthrprosergluprotyrpro
105510601065
trpargvaltyrasngluserglnilevalleuleuproglyasn
107010751080
gluthrasppheaspileasptyrserlysvalalaglumetasn
108510901095
progluasnvalaspileasppheargglyasnglnthrasptrp
110011051110
arglysglyargpheilevalleuthrvalhisaspthrtyrasp
111511201125
aspaspaspaspglulysglyalathrilelysgluphealaleu
113011351140
phepro
1145
<210>9
<211>9187
<212>dna
<213>人工序列(artificialsequence)
<220>
<223>由多种生物体乳酸克鲁维酵母和酿酒酵母共同组成的序列
<400>9
aaagccgataacaaaatctttgtcgctcttcgcaatgtcaacagtacccttagtatattc60
tccagtagatagggagcccttgcatgacaattctgctaacatcaaaaggcctctaggttc120
ctttgttacttcttctgccgcctgcttcaaaccgctaacaatacctgggcccaccacacc180
gtgtgcattcgtaatgtctgcccattctgctattctgtatacacccgcagagtactgcaa240
tttgactgtattaccaatgtcagcaaattttctgtcttcgaagagtaaaaaattgtactt300
ggcggataatgcctttagcggcttaactgtgccctccatggaaaaatcagtcaagatatc360
cacatgtgtttttagtaaacaaattttgggacctaatgcttcaactaactccagtaattc420
cttggtggtacgaacatccaatgaagcacacaagtttgtttgcttttcgtgcatgatatt480
aaatagcttggcagcaacaggactaggatgagtagcagcacgttccttatatgtagcttt540
cgacatgatttatcttcgtttcctgcaggtttttgttctgtgcagttgggttaagaatac600
tgggcaatttcatgtttcttcaacactacatatgcgtatatataccaatctaagtctgtg660
ctccttccttcgttcttccttctgttcggagattaccgaatcaaaaaaatttcaaagaaa720
ccgaaatcaaaaaaaagaataaaaaaaaaatgatgaattgaattgaaaagcgtggtgcac780
tctcagtacaatctgctctgatgccgcatagttaagccagccccgacacccgccaacacc840
cgctgacgcgccctgacgggcttgtctgctcccggcatccgcttacagacaagctgtgac900
cgtctccgggagctgcatgtgtcagaggttttcaccgtcatcaccgaaacgcgcgagacg960
aaagggcctcgtgatacgcctatttttataggttaatgtcatgataataatggtttctta1020
ggacggatcgcttgcctgtaacttacacgcgcctcgtatcttttaatgatggaataattt1080
gggaatttactctgtgtttatttatttttatgttttgtatttggattttagaaagtaaat1140
aaagaaggtagaagagttacggaatgaagaaaaaaaaataaacaaaggtttaaaaaattt1200
caacaaaaagcgtactttacatatatatttattagacaagaaaagcagattaaatagata1260
tacattcgattaacgataagtaaaatgtaaaatcacaggattttcgtgtgtggtcttcta1320
cacagacaagatgaaacaattcggcattaatacctgagagcaggaagagcaagataaaag1380
gtagtatttgttggcgatccccctagagtcttttacatcttcggaaaacaaaaactattt1440
tttctttaatttctttttttactttctatttttaatttatatatttatattaaaaaattt1500
aaattataattatttttatagcacgtgatgaaaaggacccaggtggcacttttcggggaa1560
atgtgcgcggaacccctatttgtttatttttctaaatacattcaaatatgtatccgctca1620
tgagacaataaccctgataaatgcttcaataatattgaaaaaggaagagtatgagtattc1680
aacatttccgtgtcgcccttattcccttttttgcggcattttgccttcctgtttttgctc1740
acccagaaacgctggtgaaagtaaaagatgctgaagatcagttgggtgcacgagtgggtt1800
acatcgaactggatctcaacagcggtaagatccttgagagttttcgccccgaagaacgtt1860
ttccaatgatgagcacttttaaagttctgctatgtggcgcggtattatcccgtattgacg1920
ccgggcaagagcaactcggtcgccgcatacactattctcagaatgacttggttgagtact1980
caccagtcacagaaaagcatcttacggatggcatgacagtaagagaattatgcagtgctg2040
ccataaccatgagtgataacactgcggccaacttacttctgacaacgatcggaggaccga2100
aggagctaaccgctttttttcacaacatgggggatcatgtaactcgccttgatcgttggg2160
aaccggagctgaatgaagccataccaaacgacgagcgtgacaccacgatgcctgtagcaa2220
tggcaacaacgttgcgcaaactattaactggcgaactacttactctagcttcccggcaac2280
aattaatagactggatggaggcggataaagttgcaggaccacttctgcgctcggcccttc2340
cggctggctggtttattgctgataaatctggagccggtgagcgtgggtctcgcggtatca2400
ttgcagcactggggccagatggtaagccctcccgtatcgtagttatctacacgacgggca2460
gtcaggcaactatggatgaacgaaatagacagatcgctgagataggtgcctcactgatta2520
agcattggtaactgtcagaccaagtttactcatatatactttagattgatttaaaacttc2580
atttttaatttaaaaggatctaggtgaagatcctttttgataatctcatgaccaaaatcc2640
cttaacgtgagttttcgttccactgagcgtcagaccccgtagaaaagatcaaaggatctt2700
cttgagatcctttttttctgcgcgtaatctgctgcttgcaaacaaaaaaaccaccgctac2760
cagcggtggtttgtttgccggatcaagagctaccaactctttttccgaaggtaactggct2820
tcagcagagcgcagataccaaatactgtccttctagtgtagccgtagttaggccaccact2880
tcaagaactctgtagcaccgcctacatacctcgctctgctaatcctgttaccagtggctg2940
ctgccagtggcgataagtcgtgtcttaccgggttggactcaagacgatagttaccggata3000
aggcgcagcggtcgggctgaacggggggttcgtgcacacagcccagcttggagcgaacga3060
cctacaccgaactgagatacctacagcgtgagcattgagaaagcgccacgcttcccgaag3120
ggagaaaggcggacaggtatccggtaagcggcagggtcggaacaggagagcgcacgaggg3180
agcttccaggggggaacgcctggtatctttatagtcctgtcgggtttcgccacctctgac3240
ttgagcgtcgatttttgtgatgctcgtcaggggggccgagcctatggaaaaacgccagca3300
acgcggcctttttacggttcctggccttttgctggccttttgctcacatgttctttcctg3360
cgttatcccctgattctgtggataaccgtattaccgcctttgagtgagctgataccgctc3420
gccgcagccgaacgaccgagcgcagcgagtcagtgagcgaggaagcggaagagcgcccaa3480
tacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggt3540
ttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtgagttacctcactcatt3600
aggcaccccaggctttacactttatgcttccggctcctatgttgtgtggaattgtgagcg3660
gataacaatttcacacaggaaacagctatgaccatgattacgccaagctcggaattaacc3720
ctcactaaagggaacaaaagctgggtaccgggccccccctcgagatctcccgagtttatc3780
attatcaatactgccatttcaaagaatacgtaaataattaatagtagtgattttcctaac3840
tttatttagtcaaaaaattggccttttaattctgctgtaacccgtacatgcccaaaatag3900
ggggcgggttacacagaatatataacatcataggtgtctgggtgaacagtttattcctgg3960
catccactaaatataatggagcccgctttttttaagctggcatccagaaaaaaaaagaat4020
cccagcaccaaaatattgttttcttcaccaaccatcagttcataggtccattctcttagc4080
gcaactacacagaacaggggcacaaacaggcaaaaaacgggcacaacctcaatggagtga4140
tgcaacctgcttggagtaaatgatgacacaaggcaattgacctacgcatgtatctatctc4200
attttcttacaccttctattaccttctgctctctctgatttggaaaaagctgaaaaaaaa4260
ggttgaaaccagttccctgaaattattcccctatttgactaataagtatataaagacggt4320
aggtattgattgtaattctgtaaatctatttcttaaacttcttaaattctacttttatag4380
ttagtcttttttttagttttaaaacactaagaacttagtttcgaataaacacacataaaa4440
acaaatctagaatgcttatcgtgccattcattctcttcagcgtagcaattctagctccaa4500
tatacttttacttgaccaaacctttaccattacaacatactcattttggctcttttgtct4560
gcgctgaggataccttattctgccctgaatctcagagaaaagcctctgaaaagatgtata4620
gtttattgaaggatcacgaaaacacattctacgatgaggaacagcaaatcctgggaaact4680
tattgttatcagaaaatacattctcaagacaaccatatgttgcaaacggttacattggat4740
ccagaatccctaaccttgggttcggatacgcacttgataccattaacgtttgggtaaatg4800
actcctctataccaggcgcccttgataatggttggccattgagaaaccagagatttgctg4860
gggcattcatttcagacttctactgtctacaagagaagctcaactcaactaacttccctg4920
aattggacgatgatggttactcaactgttatatcaacaataccacaatggacagatctct4980
caattttaaagcacactactacaggtcaaatcgagtacattaatcctacagatgttaagt5040
tagataagattacaaactatatgcaaaacttatcacttcaagatggaattgtaacaacta5100
catttgtttacgacaaacaactattggttactacaaaggtagttgcacatagaaagatat5160
accctctcggagtcgttaccttagaactatctttgttcgacaacaattctgatactagtg5220
ctgacaacgaaaacgaatacgtcgaattagagatctgtgattcactaaacttctctacat5280
cccacagaaccgtacttaatgattacggttacgatcaaaacaacgagggtattttcatgg5340
tggtggagccagaaaacgtcccatattctaatgcatcccttttttcatattttgatatcc5400
caagtagagatacattaacactttctaagtactccgattcaatcagtcaatgtacaacac5460
aaatcttaaaggcaaattcaacttttgtagctcacaagtacatcggtataattagctctg5520
agtacgataacaaacaacctgatgataactctaatggaacttctaatgaagcgcttaaaa5580
tgtctaacctggaacgtgcaaccagtattgtgcttgataacaaaggtaattacgattctc5640
tgattcaatcacataaaaatgcttggaagagaatttacaaagatgctagcattgaaatac5700
catccgatggtttgctggagatgacagcaaaaagttctatttaccatttactagcaaata5760
caagatctcataacgtgtctgaggacagaggtttgcctataggcgtgtcaggattatctt5820
ctgattcttacgggggtatggttttctgggattctgacttgtggattctaccagcactcc5880
tgccattcttcccaaacgctgcaagacagatcaataactacagaaatgcaagccttcacc5940
aggccaagctaaacgcagaaaagtacggctacgatggcgcgctatacccatggacatctg6000
gcagatacgcgaactgtacttcaactggtccatgtgtcgactacgaatatcatatcaacg6060
tggacatcgctttgtcatctttcgccatctacatgaatggtgatgaagatgacgaacgtt6120
ccgaagagtacttgcgttacacaacatggccattcgtagaaaacgcagcaaagatgtttg6180
ctcaatacgttaagtggaatgacacaatgcaacaatacaccactcataacttgactgatc6240
cagatgaatatgcaaacttcgttgacaacgctgcatttactaatgctggtatacaatctg6300
tgatggtctgggctcatgatatcgccagacacttgggcatcgatccagatccacagtggt6360
tggagatagccgacaacatccatattccaatttccgagacaaacatcacattggaatact6420
caggtatgaactcctctgtcgaaatcaagcaagctgatgtggtattgatgatttacccat6480
tatcttacataacagatcaaagcatcttgaacaacgcaattaaaaacttgtactactatt6540
ctgaacgtcaaagtgcatcagggcctgctatgacatacccagtatttgtcgccggagcgg6600
catctcttcttaactacggttcatcttcacaatcctatctatacaagtcagttgtcccat6660
acttacgtagtccttttgctcaattctcagaacaaagtgacgacaacttcttgaccaatg6720
gtctaacacagccagcgttcccatttctaacagctaacggtggtttcttgcagtctattc6780
tattcggtttgactgggctcagatacagttacgaagttgatgaagatacagggaaaatcc6840
atagattactgaagttcaatccaattcagttgcctctcctacctggaggtattagaatta6900
acaactttaagtacatgggtcaggtattagatgttttgatcacagacaccgagggtatca6960
tcatccacaaaaacggtactaaggaaatcagaatcaaagttccagatagaactctaattc7020
ctgatgtagatgtcaagtactcagaagagactgatcctattaagcaaattttgcatggta7080
gaagatctgttccaaccggtaaaaactacttcactatacaacctggtgatgtgttcaaga7140
caccactttacatccctaaaaaaaacttggaaggcaatttggtcgaggctaagcaaatta7200
ccaatttgactgccggcgtcccaggtgacgttgctgtctctgtaatagatggaaacaact7260
ttacacactggcaaccagcatacaaaaaccttccagccagattgttaatcgatatgggca7320
ataattttacccaagagattaagtccggcaagatcatatggggctcaagaccagcaaaaa7380
cattctccctgtcaattttgccacagacagaagaggttttcaaaaatcttacacatattt7440
tatctaacgttaatcaatattgtaacaagacaggtgatgagtgcgtcagagatttagaga7500
gaacagagaaagggttcgatgccgcaatagaagatgttttcgactggtacggtattgacc7560
cagacagcattatctcaacacatcctgaattaaaagatatgaaaacaaagttcgtcaaga7620
tcctagatcactacaaagtcactccatccgaaccttatccatggagagtttacaacgagt7680
ctcaaatcgtgctgcttccaggcaatgaaacagatttcgatattgattactctaaagttg7740
ctgaaatgaaccctgaaaacgttgatattgatttcagagggaaccagacagattggagaa7800
agggaagatttatcgttctcacagtgcatgatacttacgacgacgatgatgacgaaaagg7860
gtgccactatcaaggaatttgcattattcccttgattaattaaacaggccccttttcctt7920
tgtcgatatcatgtaattagttatgtcacgcttacattcacgccctcctcccacatccgc7980
tctaaccgaaaaggaaggagttagacaacctgaagtctaggtccctatttatttttttat8040
agttatgttagtattaagaacgttatttatatttcaaatttttcttttttttctgtacaa8100
acgcgtgtacgcatgtaacgggcagacgcggccgccaccgcggtggagctccaattcgcc8160
ctatagtgagtcgtattacaattcactggccgtcgttttacaacgtcgtgactgggaaaa8220
ccctggcgttacccaacttaatcgccttgcagcacatccccccttcgccagctggcgtaa8280
tagcgaagaggcccgcaccgatcgcccttcccaacagttgcgcagcctgaatggcgaatg8340
gcgcgacgcgccctgtagcggcgcattaagcgcggcgggtgtggtggttacgcgcagcgt8400
gaccgctacacttgccagcgccctagcgcccgctcctttcgctttcttcccttcctttct8460
cgccacgttcgccggctttccccgtcaagctctaaatcgggggctccctttagggttccg8520
atttagtgctttacggcacctcgaccccaaaaaacttgattagggtgatggttcacgtag8580
tgggccatcgccctgatagacggtttttcgccctttgacgttggagtccacgttctttaa8640
tagtggactcttgttccaaactggaacaacactcaaccctatctcggtctattcttttga8700
tttataagggattttgccgatttcggcctattggttaaaaaatgagctgatttaacaaaa8760
atttaacgcgaattttaacaaaatattaacgtttacaatttcctgatgcggtattttctc8820
cttacgcatctgtgcggtatttcacaccgcagggtaataactgatataattaaattgaag8880
ctctaatttgtgagtttagtatacatgcatttacttataatacagttttttagttttgct8940
ggccgcatcttctcaaatatgcttcccagcctgcttttctgtaacgttcaccctctacct9000
tagcatcccttccctttgcaaatagtcctcttccaacaataataatgtcagatcctgtag9060
agaccacatcatccacggttctatactgttgacccaatgcgtctcccttgtcatctaaac9120
ccacaccgggtgtcataatcaaccaatcgtaaccttcatctcttccacccatgtctcttt9180
gagcaat9187
<210>10
<211>8866
<212>dna
<213>人工序列(artificialsequence)
<220>
<223>由多种生物体近平滑假丝酵母和酿酒酵母共同组成的序列
<400>10
tgtttatttttctaaatacattcaaatatgtatccgctcatgagacaataaccctgataa60
atgcttcaataatattgaaaaaggaagagtatgagtattcaacatttccgtgtcgccctt120
attcccttttttgcggcattttgccttcctgtttttgctcacccagaaacgctggtgaaa180
gtaaaagatgctgaagatcagttgggtgcacgagtgggttacatcgaactggatctcaac240
agcggtaagatccttgagagttttcgccccgaagaacgttttccaatgatgagcactttt300
aaagttctgctatgtggcgcggtattatcccgtattgacgccgggcaagagcaactcggt360
cgccgcatacactattctcagaatgacttggttgagtactcaccagtcacagaaaagcat420
cttacggatggcatgacagtaagagaattatgcagtgctgccataaccatgagtgataac480
actgcggccaacttacttctgacaacgatcggaggaccgaaggagctaaccgcttttttt540
cacaacatgggggatcatgtaactcgccttgatcgttgggaaccggagctgaatgaagcc600
ataccaaacgacgagcgtgacaccacgatgcctgtagcaatggcaacaacgttgcgcaaa660
ctattaactggcgaactacttactctagcttcccggcaacaattaatagactggatggag720
gcggataaagttgcaggaccacttctgcgctcggcccttccggctggctggtttattgct780
gataaatctggagccggtgagcgtgggtctcgcggtatcattgcagcactggggccagat840
ggtaagccctcccgtatcgtagttatctacacgacgggcagtcaggcaactatggatgaa900
cgaaatagacagatcgctgagataggtgcctcactgattaagcattggtaactgtcagac960
caagtttactcatatatactttagattgatttaaaacttcatttttaatttaaaaggatc1020
taggtgaagatcctttttgataatctcatgaccaaaatcccttaacgtgagttttcgttc1080
cactgagcgtcagaccccgtagaaaagatcaaaggatcttcttgagatcctttttttctg1140
cgcgtaatctgctgcttgcaaacaaaaaaaccaccgctaccagcggtggtttgtttgccg1200
gatcaagagctaccaactctttttccgaaggtaactggcttcagcagagcgcagatacca1260
aatactgtccttctagtgtagccgtagttaggccaccacttcaagaactctgtagcaccg1320
cctacatacctcgctctgctaatcctgttaccagtggctgctgccagtggcgataagtcg1380
tgtcttaccgggttggactcaagacgatagttaccggataaggcgcagcggtcgggctga1440
acggggggttcgtgcacacagcccagcttggagcgaacgacctacaccgaactgagatac1500
ctacagcgtgagcattgagaaagcgccacgcttcccgaagggagaaaggcggacaggtat1560
ccggtaagcggcagggtcggaacaggagagcgcacgagggagcttccaggggggaacgcc1620
tggtatctttatagtcctgtcgggtttcgccacctctgacttgagcgtcgatttttgtga1680
tgctcgtcaggggggccgagcctatggaaaaacgccagcaacgcggcctttttacggttc1740
ctggccttttgctggccttttgctcacatgttctttcctgcgttatcccctgattctgtg1800
gataaccgtattaccgcctttgagtgagctgataccgctcgccgcagccgaacgaccgag1860
cgcagcgagtcagtgagcgaggaagcggaagagcgcccaatacgcaaaccgcctctcccc1920
gcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggc1980
agtgagcgcaacgcaattaatgtgagttacctcactcattaggcaccccaggctttacac2040
tttatgcttccggctcctatgttgtgtggaattgtgagcggataacaatttcacacagga2100
aacagctatgaccatgattacgccaagctcggaattaaccctcactaaagggaacaaaag2160
ctgggtaccgggccccccctcgagatctcccgagtttatcattatcaatactgccatttc2220
aaagaatacgtaaataattaatagtagtgattttcctaactttatttagtcaaaaaattg2280
gccttttaattctgctgtaacccgtacatgcccaaaatagggggcgggttacacagaata2340
tataacatcataggtgtctgggtgaacagtttattcctggcatccactaaatataatgga2400
gcccgctttttttaagctggcatccagaaaaaaaaagaatcccagcaccaaaatattgtt2460
ttcttcaccaaccatcagttcataggtccattctcttagcgcaactacacagaacagggg2520
cacaaacaggcaaaaaacgggcacaacctcaatggagtgatgcaacctgcttggagtaaa2580
tgatgacacaaggcaattgacctacgcatgtatctatctcattttcttacaccttctatt2640
accttctgctctctctgatttggaaaaagctgaaaaaaaaggttgaaaccagttccctga2700
aattattcccctatttgactaataagtatataaagacggtaggtattgattgtaattctg2760
taaatctatttcttaaacttcttaaattctacttttatagttagtcttttttttagtttt2820
aaaacactaagaacttagtttcgaataaacacacataaaaacaaatctagaatgaaattc2880
atcagtacctttttaacatttatcttagctgcagtctcagttacagcgagattttctgag2940
gcgttccctttcgtcttgccaagagcagaaaagtacacattggaacaaacatcagattct3000
aacttgggcgatttcgtcaactcattcgagaacaaggagatcttttcacaagttcagttc3060
tcagagcatgctttctacgatagaaaaatgaacgtagtggggactattgaatactctaag3120
tacaaccaatttcagaaacaaccatatgtagctaacggttacattggatccagaatccca3180
aacttgggtcatggtttcacatacgatcaattaactgagtctccagattccaaaaaagat3240
gacttgtataacggctggcctctgttcgataaaagattctcaggtgcctttatcgctgga3300
ttttacgatttacaggaaaatactaccggaaataacttcccagaacttttaaaaaacggt3360
tacgaatccgttattagtgccatccctcaatggactactctcaagctcgcaatccaacac3420
ggcgataagagatacactttggaccctgcaagtccagatggtgttggtcaaataactaac3480
tacgtccaaaatatgtccttaagcactggcattgttacaacacaatttacatggttagaa3540
tcactagatgtaagatatacagtgcttgctcatcgttccgagattaacttgggcctagtt3600
aaggtcgatattaaaaacaagggaaatgatacagttgaaattgatgtaatcgatgagtta3660
aacttcgatactgctcagagatgctctctgaatttgatcgggcatgataattctggtatt3720
tacgtgtctttctccccagataatctggattacatttctggagctatctacagcacacta3780
cacctcgattctgtgaagagacattctaaaaacaaaaagacaaaccagacctcaagcata3840
cgtctgcatgctcaagacagtacacagtttggaaagactgtaggtatagtttcaaccgac3900
ctggacccatctaagttcaaaaacagtcgtgacactttggaatttgcaaaaaaagtgtcc3960
caaaagttcgaatacttggacttagttattaagagtcatgtcagagcgtggaaggaacta4020
atgggcaagcaaagtacaataacttttccaaatgatccattgcttgatttgggatctaag4080
gcttcagtttatcacttgctagctaacaccagaccagatgcacaaggcctaactggtgct4140
ctcggcgtcgctggcctttcttctgattcatacggtggcatggtgttctgggacaccgat4200
ttctggatgttcagagcgatactggccctagatcctacacacgcaaagtcaatagtcaac4260
tacagaatgcatacacaccaacaagcactcaaaaacacacctcagggttacgaaggggcc4320
gcttacccttggacttctggtcgttttggcaactgcacagccaccgggccttgtttaaat4380
tacgaatatcacattaactcagccatagcaatggctgcttgggaattgtacatcagtgga4440
gcagtagacgatcaatacttagagagtacagtctacccattgatttcagacgctgcaaag4500
ttctacgccgattacgtaacatcatataacgaaactatcgacaagttcattacatcaaac4560
ctaaccgacccagatgaatacgctaatcacgtagacaatggtgcctatacaaatgccggt4620
attgcccttcttatgaattggatcaatgacatcggcgacatcctggggattaagatgcca4680
gaaatttacactgatatatctcaaaagatgtatctcccaactgcggataattctcaaaac4740
attaccttagaatactctggtatgaactcctctgtcggaatcaaacaagctgatgttgtc4800
cttatgacatacccacttcagaactctctagttgacgatgaacaggcatacattaacatg4860
gagttttactcaggaaaacaagtttcttacggaccagcaatgaccttctctatattttct4920
attgtcgcagcaaacttggctacatctggttgtgcctcacaatcttacttgcacaaagca4980
atgcaaccatatcttagaggccctttcgcacaattctccgaacaaaataatgacaacttc5040
aagacaaacggtggaacacatccagcttatccatttcttactgcacatggtgggttcata5100
caggctgttgtgaacggacttactgggcttagacattcctacacaatcgataatggtaag5160
attcgtagatcactattcttagatcctatcgccttaccttgccttggcgatggggttcaa5220
tactccggtattcattacgataatcatacaattagcatgaacattacaggttccaccttc5280
gttatcagaaacgagggcaaaaccaacaagagagcaaacgactatatctctatcactatt5340
gccgatagaaatccaggtagaggtacatacaagttgaaggacaatgaagagaaaagcttc5400
aagttataccaaccttctaagatttttgaagattctgtgtcagagtgtggtttggcaact5460
ttctacaatataacagaatcagctcctggtgacagctctttctccataaacgatggtgat5520
aacactaccagatggcaagttaagtacaatgatacaacaggtaaggtcttgatcgatttg5580
cattcaatcaaaaacatcactggtgttgttttcaactgggctgataagccacctaaaaag5640
gtaaagttgagtaagtacatggggactaagtttaaggatgtcactgactttttggctaag5700
gttgatttcggtaattacgtttacaaaaactacagattcgctaaccctggtgaaaaactt5760
ttgaaccaatcagaagtgttcgaaaacgtttactctgatgatgttacaatcaacgcacca5820
ttctcttctaaggaactagaggaagttatcgttccagtgagacataatactacatcttgg5880
aacattaacttacaaggcagattcttattgttagaagtggacggaatccacaacaccatt5940
ccaatcgaagatgattacggtggcgcaaagttagccgaagtgaacttcttttagttaatt6000
aaacaggccccttttcctttgtcgatatcatgtaattagttatgtcacgcttacattcac6060
gccctcctcccacatccgctctaaccgaaaaggaaggagttagacaacctgaagtctagg6120
tccctatttatttttttatagttatgttagtattaagaacgttatttatatttcaaattt6180
ttcttttttttctgtacaaacgcgtgtacgcatgtaacgggcagacgcggccgccaccgc6240
ggtggagctccaattcgccctatagtgagtcgtattacaattcactggccgtcgttttac6300
aacgtcgtgactgggaaaaccctggcgttacccaacttaatcgccttgcagcacatcccc6360
ccttcgccagctggcgtaatagcgaagaggcccgcaccgatcgcccttcccaacagttgc6420
gcagcctgaatggcgaatggcgcgacgcgccctgtagcggcgcattaagcgcggcgggtg6480
tggtggttacgcgcagcgtgaccgctacacttgccagcgccctagcgcccgctcctttcg6540
ctttcttcccttcctttctcgccacgttcgccggctttccccgtcaagctctaaatcggg6600
ggctccctttagggttccgatttagtgctttacggcacctcgaccccaaaaaacttgatt6660
agggtgatggttcacgtagtgggccatcgccctgatagacggtttttcgccctttgacgt6720
tggagtccacgttctttaatagtggactcttgttccaaactggaacaacactcaacccta6780
tctcggtctattcttttgatttataagggattttgccgatttcggcctattggttaaaaa6840
atgagctgatttaacaaaaatttaacgcgaattttaacaaaatattaacgtttacaattt6900
cctgatgcggtattttctccttacgcatctgtgcggtatttcacaccgcagggtaataac6960
tgatataattaaattgaagctctaatttgtgagtttagtatacatgcatttacttataat7020
acagttttttagttttgctggccgcatcttctcaaatatgcttcccagcctgcttttctg7080
taacgttcaccctctaccttagcatcccttccctttgcaaatagtcctcttccaacaata7140
ataatgtcagatcctgtagagaccacatcatccacggttctatactgttgacccaatgcg7200
tctcccttgtcatctaaacccacaccgggtgtcataatcaaccaatcgtaaccttcatct7260
cttccacccatgtctctttgagcaataaagccgataacaaaatctttgtcgctcttcgca7320
atgtcaacagtacccttagtatattctccagtagatagggagcccttgcatgacaattct7380
gctaacatcaaaaggcctctaggttcctttgttacttcttctgccgcctgcttcaaaccg7440
ctaacaatacctgggcccaccacaccgtgtgcattcgtaatgtctgcccattctgctatt7500
ctgtatacacccgcagagtactgcaatttgactgtattaccaatgtcagcaaattttctg7560
tcttcgaagagtaaaaaattgtacttggcggataatgcctttagcggcttaactgtgccc7620
tccatggaaaaatcagtcaagatatccacatgtgtttttagtaaacaaattttgggacct7680
aatgcttcaactaactccagtaattccttggtggtacgaacatccaatgaagcacacaag7740
tttgtttgcttttcgtgcatgatattaaatagcttggcagcaacaggactaggatgagta7800
gcagcacgttccttatatgtagctttcgacatgatttatcttcgtttcctgcaggttttt7860
gttctgtgcagttgggttaagaatactgggcaatttcatgtttcttcaacactacatatg7920
cgtatatataccaatctaagtctgtgctccttccttcgttcttccttctgttcggagatt7980
accgaatcaaaaaaatttcaaagaaaccgaaatcaaaaaaaagaataaaaaaaaaatgat8040
gaattgaattgaaaagcgtggtgcactctcagtacaatctgctctgatgccgcatagtta8100
agccagccccgacacccgccaacacccgctgacgcgccctgacgggcttgtctgctcccg8160
gcatccgcttacagacaagctgtgaccgtctccgggagctgcatgtgtcagaggttttca8220
ccgtcatcaccgaaacgcgcgagacgaaagggcctcgtgatacgcctatttttataggtt8280
aatgtcatgataataatggtttcttaggacggatcgcttgcctgtaacttacacgcgcct8340
cgtatcttttaatgatggaataatttgggaatttactctgtgtttatttatttttatgtt8400
ttgtatttggattttagaaagtaaataaagaaggtagaagagttacggaatgaagaaaaa8460
aaaataaacaaaggtttaaaaaatttcaacaaaaagcgtactttacatatatatttatta8520
gacaagaaaagcagattaaatagatatacattcgattaacgataagtaaaatgtaaaatc8580
acaggattttcgtgtgtggtcttctacacagacaagatgaaacaattcggcattaatacc8640
tgagagcaggaagagcaagataaaaggtagtatttgttggcgatccccctagagtctttt8700
acatcttcggaaaacaaaaactattttttctttaatttctttttttactttctattttta8760
atttatatatttatattaaaaaatttaaattataattatttttatagcacgtgatgaaaa8820
ggacccaggtggcacttttcggggaaatgtgcgcggaacccctatt8866
<210>11
<211>1040
<212>prt
<213>人工序列(artificialsequence)
<220>
<223>由多种生物体近平滑假丝酵母和酿酒酵母共同组成的序列
<400>11
metlyspheileserthrpheleuthrpheileleualaalavalser
151015
valthralaargpheserglualapheprophevalleuproargala
202530
glulystyrthrleugluglnthrseraspserasnleuglyaspphe
354045
valasnserphegluasnlysgluilepheserglnvalglnpheser
505560
gluhisalaphetyrasparglysmetasnvalvalglythrileglu
65707580
tyrserlystyrasnglnpheglnlysglnprotyrvalalaasngly
859095
tyrileglyserargileproasnleuglyhisglyphethrtyrasp
100105110
glnleuthrgluserproaspserlyslysaspaspleutyrasngly
115120125
trpproleupheasplysargpheserglyalapheilealaglyphe
130135140
tyraspleuglngluasnthrthrglyasnasnpheprogluleuleu
145150155160
lysasnglytyrgluservalileseralaileproglntrpthrthr
165170175
leulysleualaileglnhisglyasplysargtyrthrleuasppro
180185190
alaserproaspglyvalglyglnilethrasntyrvalglnasnmet
195200205
serleuserthrglyilevalthrthrglnphethrtrpleugluser
210215220
leuaspvalargtyrthrvalleualahisargsergluileasnleu
225230235240
glyleuvallysvalaspilelysasnlysglyasnaspthrvalglu
245250255
ileaspvalileaspgluleuasnpheaspthralaglnargcysser
260265270
leuasnleuileglyhisaspasnserglyiletyrvalserpheser
275280285
proaspasnleuasptyrileserglyalailetyrserthrleuhis
290295300
leuaspservallysarghisserlysasnlyslysthrasnglnthr
305310315320
serserileargleuhisalaglnaspserthrglnpheglylysthr
325330335
valglyilevalserthraspleuaspproserlysphelysasnser
340345350
argaspthrleugluphealalyslysvalserglnlyspheglutyr
355360365
leuaspleuvalilelysserhisvalargalatrplysgluleumet
370375380
glylysglnserthrilethrpheproasnaspproleuleuaspleu
385390395400
glyserlysalaservaltyrhisleuleualaasnthrargproasp
405410415
alaglnglyleuthrglyalaleuglyvalalaglyleuserserasp
420425430
sertyrglyglymetvalphetrpaspthraspphetrpmetphearg
435440445
alaileleualaleuaspprothrhisalalysserilevalasntyr
450455460
argmethisthrhisglnglnalaleulysasnthrproglnglytyr
465470475480
gluglyalaalatyrprotrpthrserglyargpheglyasncysthr
485490495
alathrglyprocysleuasntyrglutyrhisileasnseralaile
500505510
alametalaalatrpgluleutyrileserglyalavalaspaspgln
515520525
tyrleugluserthrvaltyrproleuileseraspalaalalysphe
530535540
tyralaasptyrvalthrsertyrasngluthrileasplyspheile
545550555560
thrserasnleuthraspproaspglutyralaasnhisvalaspasn
565570575
glyalatyrthrasnalaglyilealaleuleumetasntrpileasn
580585590
aspileglyaspileleuglyilelysmetprogluiletyrthrasp
595600605
ileserglnlysmettyrleuprothralaaspasnserglnasnile
610615620
thrleuglutyrserglymetasnserservalglyilelysglnala
625630635640
aspvalvalleumetthrtyrproleuglnasnserleuvalaspasp
645650655
gluglnalatyrileasnmetgluphetyrserglylysglnvalser
660665670
tyrglyproalametthrpheserilepheserilevalalaalaasn
675680685
leualathrserglycysalaserglnsertyrleuhislysalamet
690695700
glnprotyrleuargglyprophealaglnphesergluglnasnasn
705710715720
aspasnphelysthrasnglyglythrhisproalatyrpropheleu
725730735
thralahisglyglypheileglnalavalvalasnglyleuthrgly
740745750
leuarghissertyrthrileaspasnglylysileargargserleu
755760765
pheleuaspproilealaleuprocysleuglyaspglyvalglntyr
770775780
serglyilehistyraspasnhisthrilesermetasnilethrgly
785790795800
serthrphevalileargasngluglylysthrasnlysargalaasn
805810815
asptyrileserilethrilealaaspargasnproglyargglythr
820825830
tyrlysleulysaspasngluglulysserphelysleutyrglnpro
835840845
serlysilephegluaspservalserglucysglyleualathrphe
850855860
tyrasnilethrgluseralaproglyaspserserpheserileasn
865870875880
aspglyaspasnthrthrargtrpglnvallystyrasnaspthrthr
885890895
glylysvalleuileaspleuhisserilelysasnilethrglyval
900905910
valpheasntrpalaasplysproprolyslysvallysleuserlys
915920925
tyrmetglythrlysphelysaspvalthrasppheleualalysval
930935940
asppheglyasntyrvaltyrlysasntyrargphealaasnprogly
945950955960
glulysleuleuasnglnsergluvalphegluasnvaltyrserasp
965970975
aspvalthrileasnalapropheserserlysgluleuglugluval
980985990
ilevalprovalarghisasnthrthrsertrpasnileasnleugln
99510001005
glyargpheleuleuleugluvalaspglyilehisasnthrile
101010151020
proilegluaspasptyrglyglyalalysleualagluvalasn
102510301035
phephe
1040
<210>12
<211>8959
<212>dna
<213>人工序列(artificialsequence)
<220>
<223>由多种生物体近平滑假丝酵母和酿酒酵母共同组成的序列
<400>12
tgggcccaccacaccgtgtgcattcgtaatgtctgcccattctgctattctgtatacacc60
cgcagagtactgcaatttgactgtattaccaatgtcagcaaattttctgtcttcgaagag120
taaaaaattgtacttggcggataatgcctttagcggcttaactgtgccctccatggaaaa180
atcagtcaagatatccacatgtgtttttagtaaacaaattttgggacctaatgcttcaac240
taactccagtaattccttggtggtacgaacatccaatgaagcacacaagtttgtttgctt300
ttcgtgcatgatattaaatagcttggcagcaacaggactaggatgagtagcagcacgttc360
cttatatgtagctttcgacatgatttatcttcgtttcctgcaggtttttgttctgtgcag420
ttgggttaagaatactgggcaatttcatgtttcttcaacactacatatgcgtatatatac480
caatctaagtctgtgctccttccttcgttcttccttctgttcggagattaccgaatcaaa540
aaaatttcaaagaaaccgaaatcaaaaaaaagaataaaaaaaaaatgatgaattgaattg600
aaaagcgtggtgcactctcagtacaatctgctctgatgccgcatagttaagccagccccg660
acacccgccaacacccgctgacgcgccctgacgggcttgtctgctcccggcatccgctta720
cagacaagctgtgaccgtctccgggagctgcatgtgtcagaggttttcaccgtcatcacc780
gaaacgcgcgagacgaaagggcctcgtgatacgcctatttttataggttaatgtcatgat840
aataatggtttcttaggacggatcgcttgcctgtaacttacacgcgcctcgtatctttta900
atgatggaataatttgggaatttactctgtgtttatttatttttatgttttgtatttgga960
ttttagaaagtaaataaagaaggtagaagagttacggaatgaagaaaaaaaaataaacaa1020
aggtttaaaaaatttcaacaaaaagcgtactttacatatatatttattagacaagaaaag1080
cagattaaatagatatacattcgattaacgataagtaaaatgtaaaatcacaggattttc1140
gtgtgtggtcttctacacagacaagatgaaacaattcggcattaatacctgagagcagga1200
agagcaagataaaaggtagtatttgttggcgatccccctagagtcttttacatcttcgga1260
aaacaaaaactattttttctttaatttctttttttactttctatttttaatttatatatt1320
tatattaaaaaatttaaattataattatttttatagcacgtgatgaaaaggacccaggtg1380
gcacttttcggggaaatgtgcgcggaacccctatttgtttatttttctaaatacattcaa1440
atatgtatccgctcatgagacaataaccctgataaatgcttcaataatattgaaaaagga1500
agagtatgagtattcaacatttccgtgtcgcccttattcccttttttgcggcattttgcc1560
ttcctgtttttgctcacccagaaacgctggtgaaagtaaaagatgctgaagatcagttgg1620
gtgcacgagtgggttacatcgaactggatctcaacagcggtaagatccttgagagttttc1680
gccccgaagaacgttttccaatgatgagcacttttaaagttctgctatgtggcgcggtat1740
tatcccgtattgacgccgggcaagagcaactcggtcgccgcatacactattctcagaatg1800
acttggttgagtactcaccagtcacagaaaagcatcttacggatggcatgacagtaagag1860
aattatgcagtgctgccataaccatgagtgataacactgcggccaacttacttctgacaa1920
cgatcggaggaccgaaggagctaaccgctttttttcacaacatgggggatcatgtaactc1980
gccttgatcgttgggaaccggagctgaatgaagccataccaaacgacgagcgtgacacca2040
cgatgcctgtagcaatggcaacaacgttgcgcaaactattaactggcgaactacttactc2100
tagcttcccggcaacaattaatagactggatggaggcggataaagttgcaggaccacttc2160
tgcgctcggcccttccggctggctggtttattgctgataaatctggagccggtgagcgtg2220
ggtctcgcggtatcattgcagcactggggccagatggtaagccctcccgtatcgtagtta2280
tctacacgacgggcagtcaggcaactatggatgaacgaaatagacagatcgctgagatag2340
gtgcctcactgattaagcattggtaactgtcagaccaagtttactcatatatactttaga2400
ttgatttaaaacttcatttttaatttaaaaggatctaggtgaagatcctttttgataatc2460
tcatgaccaaaatcccttaacgtgagttttcgttccactgagcgtcagaccccgtagaaa2520
agatcaaaggatcttcttgagatcctttttttctgcgcgtaatctgctgcttgcaaacaa2580
aaaaaccaccgctaccagcggtggtttgtttgccggatcaagagctaccaactctttttc2640
cgaaggtaactggcttcagcagagcgcagataccaaatactgtccttctagtgtagccgt2700
agttaggccaccacttcaagaactctgtagcaccgcctacatacctcgctctgctaatcc2760
tgttaccagtggctgctgccagtggcgataagtcgtgtcttaccgggttggactcaagac2820
gatagttaccggataaggcgcagcggtcgggctgaacggggggttcgtgcacacagccca2880
gcttggagcgaacgacctacaccgaactgagatacctacagcgtgagcattgagaaagcg2940
ccacgcttcccgaagggagaaaggcggacaggtatccggtaagcggcagggtcggaacag3000
gagagcgcacgagggagcttccaggggggaacgcctggtatctttatagtcctgtcgggt3060
ttcgccacctctgacttgagcgtcgatttttgtgatgctcgtcaggggggccgagcctat3120
ggaaaaacgccagcaacgcggcctttttacggttcctggccttttgctggccttttgctc3180
acatgttctttcctgcgttatcccctgattctgtggataaccgtattaccgcctttgagt3240
gagctgataccgctcgccgcagccgaacgaccgagcgcagcgagtcagtgagcgaggaag3300
cggaagagcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgca3360
gctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtga3420
gttacctcactcattaggcaccccaggctttacactttatgcttccggctcctatgttgt3480
gtggaattgtgagcggataacaatttcacacaggaaacagctatgaccatgattacgcca3540
agctcggaattaaccctcactaaagggaacaaaagctgggtaccgggccccccctcgaga3600
tctcccgagtttatcattatcaatactgccatttcaaagaatacgtaaataattaatagt3660
agtgattttcctaactttatttagtcaaaaaattggccttttaattctgctgtaacccgt3720
acatgcccaaaatagggggcgggttacacagaatatataacatcataggtgtctgggtga3780
acagtttattcctggcatccactaaatataatggagcccgctttttttaagctggcatcc3840
agaaaaaaaaagaatcccagcaccaaaatattgttttcttcaccaaccatcagttcatag3900
gtccattctcttagcgcaactacacagaacaggggcacaaacaggcaaaaaacgggcaca3960
acctcaatggagtgatgcaacctgcttggagtaaatgatgacacaaggcaattgacctac4020
gcatgtatctatctcattttcttacaccttctattaccttctgctctctctgatttggaa4080
aaagctgaaaaaaaaggttgaaaccagttccctgaaattattcccctatttgactaataa4140
gtatataaagacggtaggtattgattgtaattctgtaaatctatttcttaaacttcttaa4200
attctacttttatagttagtcttttttttagttttaaaacactaagaacttagtttcgaa4260
taaacacacataaaaacaaatctagaatggacatcgaaaacaatggtatttacatggaaa4320
atagcagtagaatcaagggtatacgtagattattcaaagacaacaagacatatatcattg4380
ccatgactatcatagcaaatttcattctgttttactctctacacttacttaataccagat4440
tttctgaggcgttccctttcgtcttgccaagagcagaaaagtacacattggaacaaacat4500
cagattctaacttgggcgatttcgtcaactcattcgagaacaaggagatcttttcacaag4560
ttcagttctcagagcatgctttctacgatagaaaaatgaacgtagtggggactattgaat4620
actctaagtacaaccaatttcagaaacaaccatatgtagctaacggttacattggatcca4680
gaatcccaaacttgggtcatggtttcacatacgatcaattaactgagtctccagattcca4740
aaaaagatgacttgtataacggctggcctctgttcgataaaagattctcaggtgccttta4800
tcgctggattttacgatttacaggaaaatactaccggaaataacttcccagaacttttaa4860
aaaacggttacgaatccgttattagtgccatccctcaatggactactctcaagctcgcaa4920
tccaacacggcgataagagatacactttggaccctgcaagtccagatggtgttggtcaaa4980
taactaactacgtccaaaatatgtccttaagcactggcattgttacaacacaatttacat5040
ggttagaatcactagatgtaagatatacagtgcttgctcatcgttccgagattaacttgg5100
gcctagttaaggtcgatattaaaaacaagggaaatgatacagttgaaattgatgtaatcg5160
atgagttaaacttcgatactgctcagagatgctctctgaatttgatcgggcatgataatt5220
ctggtatttacgtgtctttctccccagataatctggattacatttctggagctatctaca5280
gcacactacacctcgattctgtgaagagacattctaaaaacaaaaagacaaaccagacct5340
caagcatacgtctgcatgctcaagacagtacacagtttggaaagactgtaggtatagttt5400
caaccgacctggacccatctaagttcaaaaacagtcgtgacactttggaatttgcaaaaa5460
aagtgtcccaaaagttcgaatacttggacttagttattaagagtcatgtcagagcgtgga5520
aggaactaatgggcaagcaaagtacaataacttttccaaatgatccattgcttgatttgg5580
gatctaaggcttcagtttatcacttgctagctaacaccagaccagatgcacaaggcctaa5640
ctggtgctctcggcgtcgctggcctttcttctgattcatacggtggcatggtgttctggg5700
acaccgatttctggatgttcagagcgatactggccctagatcctacacacgcaaagtcaa5760
tagtcaactacagaatgcatacacaccaacaagcactcaaaaacacacctcagggttacg5820
aaggggccgcttacccttggacttctggtcgttttggcaactgcacagccaccgggcctt5880
gtttaaattacgaatatcacattaactcagccatagcaatggctgcttgggaattgtaca5940
tcagtggagcagtagacgatcaatacttagagagtacagtctacccattgatttcagacg6000
ctgcaaagttctacgccgattacgtaacatcatataacgaaactatcgacaagttcatta6060
catcaaacctaaccgacccagatgaatacgctaatcacgtagacaatggtgcctatacaa6120
atgccggtattgcccttcttatgaattggatcaatgacatcggcgacatcctggggatta6180
agatgccagaaatttacactgatatatctcaaaagatgtatctcccaactgcggataatt6240
ctcaaaacattaccttagaatactctggtatgaactcctctgtcggaatcaaacaagctg6300
atgttgtccttatgacatacccacttcagaactctctagttgacgatgaacaggcataca6360
ttaacatggagttttactcaggaaaacaagtttcttacggaccagcaatgaccttctcta6420
tattttctattgtcgcagcaaacttggctacatctggttgtgcctcacaatcttacttgc6480
acaaagcaatgcaaccatatcttagaggccctttcgcacaattctccgaacaaaataatg6540
acaacttcaagacaaacggtggaacacatccagcttatccatttcttactgcacatggtg6600
ggttcatacaggctgttgtgaacggacttactgggcttagacattcctacacaatcgata6660
atggtaagattcgtagatcactattcttagatcctatcgccttaccttgccttggcgatg6720
gggttcaatactccggtattcattacgataatcatacaattagcatgaacattacaggtt6780
ccaccttcgttatcagaaacgagggcaaaaccaacaagagagcaaacgactatatctcta6840
tcactattgccgatagaaatccaggtagaggtacatacaagttgaaggacaatgaagaga6900
aaagcttcaagttataccaaccttctaagatttttgaagattctgtgtcagagtgtggtt6960
tggcaactttctacaatataacagaatcagctcctggtgacagctctttctccataaacg7020
atggtgataacactaccagatggcaagttaagtacaatgatacaacaggtaaggtcttga7080
tcgatttgcattcaatcaaaaacatcactggtgttgttttcaactgggctgataagccac7140
ctaaaaaggtaaagttgagtaagtacatggggactaagtttaaggatgtcactgactttt7200
tggctaaggttgatttcggtaattacgtttacaaaaactacagattcgctaaccctggtg7260
aaaaacttttgaaccaatcagaagtgttcgaaaacgtttactctgatgatgttacaatca7320
acgcaccattctcttctaaggaactagaggaagttatcgttccagtgagacataatacta7380
catcttggaacattaacttacaaggcagattcttattgttagaagtggacggaatccaca7440
acaccattccaatcgaagatgattacggtggcgcaaagttagccgaagtgaacttctttt7500
agttaattaaacaggccccttttcctttgtcgatatcatgtaattagttatgtcacgctt7560
acattcacgccctcctcccacatccgctctaaccgaaaaggaaggagttagacaacctga7620
agtctaggtccctatttatttttttatagttatgttagtattaagaacgttatttatatt7680
tcaaatttttcttttttttctgtacaaacgcgtgtacgcatgtaacgggcagacgcggcc7740
gccaccgcggtggagctccaattcgccctatagtgagtcgtattacaattcactggccgt7800
cgttttacaacgtcgtgactgggaaaaccctggcgttacccaacttaatcgccttgcagc7860
acatccccccttcgccagctggcgtaatagcgaagaggcccgcaccgatcgcccttccca7920
acagttgcgcagcctgaatggcgaatggcgcgacgcgccctgtagcggcgcattaagcgc7980
ggcgggtgtggtggttacgcgcagcgtgaccgctacacttgccagcgccctagcgcccgc8040
tcctttcgctttcttcccttcctttctcgccacgttcgccggctttccccgtcaagctct8100
aaatcgggggctccctttagggttccgatttagtgctttacggcacctcgaccccaaaaa8160
acttgattagggtgatggttcacgtagtgggccatcgccctgatagacggtttttcgccc8220
tttgacgttggagtccacgttctttaatagtggactcttgttccaaactggaacaacact8280
caaccctatctcggtctattcttttgatttataagggattttgccgatttcggcctattg8340
gttaaaaaatgagctgatttaacaaaaatttaacgcgaattttaacaaaatattaacgtt8400
tacaatttcctgatgcggtattttctccttacgcatctgtgcggtatttcacaccgcagg8460
gtaataactgatataattaaattgaagctctaatttgtgagtttagtatacatgcattta8520
cttataatacagttttttagttttgctggccgcatcttctcaaatatgcttcccagcctg8580
cttttctgtaacgttcaccctctaccttagcatcccttccctttgcaaatagtcctcttc8640
caacaataataatgtcagatcctgtagagaccacatcatccacggttctatactgttgac8700
ccaatgcgtctcccttgtcatctaaacccacaccgggtgtcataatcaaccaatcgtaac8760
cttcatctcttccacccatgtctctttgagcaataaagccgataacaaaatctttgtcgc8820
tcttcgcaatgtcaacagtacccttagtatattctccagtagatagggagcccttgcatg8880
acaattctgctaacatcaaaaggcctctaggttcctttgttacttcttctgccgcctgct8940
tcaaaccgctaacaatacc8959
<210>13
<211>9130
<212>dna
<213>人工序列(artificialsequence)
<220>
<223>由多种生物体光滑假丝酵母和酿酒酵母共同组成的序列
<400>13
tgtttatttttctaaatacattcaaatatgtatccgctcatgagacaataaccctgataa60
atgcttcaataatattgaaaaaggaagagtatgagtattcaacatttccgtgtcgccctt120
attcccttttttgcggcattttgccttcctgtttttgctcacccagaaacgctggtgaaa180
gtaaaagatgctgaagatcagttgggtgcacgagtgggttacatcgaactggatctcaac240
agcggtaagatccttgagagttttcgccccgaagaacgttttccaatgatgagcactttt300
aaagttctgctatgtggcgcggtattatcccgtattgacgccgggcaagagcaactcggt360
cgccgcatacactattctcagaatgacttggttgagtactcaccagtcacagaaaagcat420
cttacggatggcatgacagtaagagaattatgcagtgctgccataaccatgagtgataac480
actgcggccaacttacttctgacaacgatcggaggaccgaaggagctaaccgcttttttt540
cacaacatgggggatcatgtaactcgccttgatcgttgggaaccggagctgaatgaagcc600
ataccaaacgacgagcgtgacaccacgatgcctgtagcaatggcaacaacgttgcgcaaa660
ctattaactggcgaactacttactctagcttcccggcaacaattaatagactggatggag720
gcggataaagttgcaggaccacttctgcgctcggcccttccggctggctggtttattgct780
gataaatctggagccggtgagcgtgggtctcgcggtatcattgcagcactggggccagat840
ggtaagccctcccgtatcgtagttatctacacgacgggcagtcaggcaactatggatgaa900
cgaaatagacagatcgctgagataggtgcctcactgattaagcattggtaactgtcagac960
caagtttactcatatatactttagattgatttaaaacttcatttttaatttaaaaggatc1020
taggtgaagatcctttttgataatctcatgaccaaaatcccttaacgtgagttttcgttc1080
cactgagcgtcagaccccgtagaaaagatcaaaggatcttcttgagatcctttttttctg1140
cgcgtaatctgctgcttgcaaacaaaaaaaccaccgctaccagcggtggtttgtttgccg1200
gatcaagagctaccaactctttttccgaaggtaactggcttcagcagagcgcagatacca1260
aatactgtccttctagtgtagccgtagttaggccaccacttcaagaactctgtagcaccg1320
cctacatacctcgctctgctaatcctgttaccagtggctgctgccagtggcgataagtcg1380
tgtcttaccgggttggactcaagacgatagttaccggataaggcgcagcggtcgggctga1440
acggggggttcgtgcacacagcccagcttggagcgaacgacctacaccgaactgagatac1500
ctacagcgtgagcattgagaaagcgccacgcttcccgaagggagaaaggcggacaggtat1560
ccggtaagcggcagggtcggaacaggagagcgcacgagggagcttccaggggggaacgcc1620
tggtatctttatagtcctgtcgggtttcgccacctctgacttgagcgtcgatttttgtga1680
tgctcgtcaggggggccgagcctatggaaaaacgccagcaacgcggcctttttacggttc1740
ctggccttttgctggccttttgctcacatgttctttcctgcgttatcccctgattctgtg1800
gataaccgtattaccgcctttgagtgagctgataccgctcgccgcagccgaacgaccgag1860
cgcagcgagtcagtgagcgaggaagcggaagagcgcccaatacgcaaaccgcctctcccc1920
gcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggc1980
agtgagcgcaacgcaattaatgtgagttacctcactcattaggcaccccaggctttacac2040
tttatgcttccggctcctatgttgtgtggaattgtgagcggataacaatttcacacagga2100
aacagctatgaccatgattacgccaagctcggaattaaccctcactaaagggaacaaaag2160
ctgggtaccgggccccccctcgagatctcccgagtttatcattatcaatactgccatttc2220
aaagaatacgtaaataattaatagtagtgattttcctaactttatttagtcaaaaaattg2280
gccttttaattctgctgtaacccgtacatgcccaaaatagggggcgggttacacagaata2340
tataacatcataggtgtctgggtgaacagtttattcctggcatccactaaatataatgga2400
gcccgctttttttaagctggcatccagaaaaaaaaagaatcccagcaccaaaatattgtt2460
ttcttcaccaaccatcagttcataggtccattctcttagcgcaactacacagaacagggg2520
cacaaacaggcaaaaaacgggcacaacctcaatggagtgatgcaacctgcttggagtaaa2580
tgatgacacaaggcaattgacctacgcatgtatctatctcattttcttacaccttctatt2640
accttctgctctctctgatttggaaaaagctgaaaaaaaaggttgaaaccagttccctga2700
aattattcccctatttgactaataagtatataaagacggtaggtattgattgtaattctg2760
taaatctatttcttaaacttcttaaattctacttttatagttagtcttttttttagtttt2820
aaaacactaagaacttagtttcgaataaacacacataaaaacaaatctagaatgaaattc2880
atctctactttccttacttttattttagctgccgtatctgtaaccgcctctaccaactct2940
tacttctcttcctccttagcttctgcttcaccattattcaacaaagagggcagagtggtt3000
agaccaatgcgtgaaagtaacttaggcctacacgcagatcctcaaactagaaaatccagt3060
aagacactttacgatttgttgtcagactttgacaatgcattttacgatgatgaaaacatg3120
atcttaggatccctagccttcggtgagaatacctactccagacagccatacgtcgcaaac3180
gggtacataggtagccgtataccaaacatcggtttcggatacgcacttgatacacttaac3240
ttgtacgctgatgcaccaggcgcacttaacaacggttggcctttgagaaacagaagattc3300
gctggaagtttcgtttctgatttctactcactgcaagccaagttgaacagtaccaatttt3360
cctgagttagatgaaaagggttatacaactgtcatttcatcaatcccagaatggacagat3420
ttgcaattcactgtagaccttaacggcactaactggttcaatccacaatcagttctaatc3480
gatgatgttattaattacaatcagaacttgagcatgaaggatggaattgttagcacaaac3540
atggattggttgaacggaatgataaacattaagtctgaagtctgggcacacagaaagatc3600
cattccttgggtatcacacgtctggaaatctcattgaaccttgatgctttacctgacgag3660
ttcactgaactaccagttacaatttacgatatcatagatttcaacacttctcatagaaca3720
acactatatgagaaaggtcaagatgaagataacaaagcgatttacatgattgtaaaccca3780
gaaaacgtaccatactcaaacgcagttgtgtactctacttgtacaatcaagggcactgaa3840
aacaacttttctccttacaacttcacatcagatgatagaattgcgagaaactacatgaca3900
aacctaacagaagagaacccaaaagtcgttatttacaagtacacatcagtggtttcctca3960
gaatataataatgacgagccaaatcctaacgttaacttgaagttcgcctcaaacatcgct4020
aacaccgcaaaaggaaactataagtctttattgtccaatcacaaaagagcttggtacgat4080
ttgtacaatgatgcctttatcgaaattccatctgatagtttattggaaatgaccgcaaga4140
tctagtctcttccatttgcttgcgaacacacgtcaatacaacgtttctacaactagagga4200
ttaccagtcggcgttggtggtctttcttcagactcttacggggggatggtcttttgggac4260
gcagacgtatggatggctccagctctcctacctttctttccaaacatagcaatgaacatg4320
aataactacagaaacgctactcatcaacaagccattgaaaatgctaagcaatacaattac4380
ccaggcgccgtttacccttggacatctggtagatatgcgaattgtacctctaccggtcct4440
tgcatcgactacgaataccatattaatgtagatatcgctttggcaagtttctctatttac4500
atgaacggtgccgagggtgccgacgaagattaccttagattcacaacatggccaatggtc4560
aaggatgctgcagtgttcttcaaggcctacgtcaaatacaatgaaacacttggagagtac4620
gaaacttataatttaactgacccagatgaatttgctaatcatgtgaataatggagctttc4680
acaaatgcaggaatcaaaactttgttgaaatgggcaacagacattggtactcaccttggg4740
gaggaagttgatcctaagtggatggaaattgctgataacatacatattcctagaagtgac4800
tctaacattactttggagtactctggcatgaatagctccgttgagattaagcaggctgac4860
gttacattgatggtataccctttaggttacatcaacgatgaatctatactaaacaacgca4920
atcaaggatttgtattactactctgaacgtcagtctgcctctgggccagctatgacttac4980
ccagtgttcgtcgcagctgccgcttccctcttgaaccacggttcatcatcacagagttac5040
ctttacaaatctgttcttccatatctgagatcaccatttgctcaatttagcgaacaatca5100
gatgacaatttcttaactaacggtttgacacaaccagcattcccatttttgaccgctaat5160
ggtggtttcttgcaatctatattatttggattaaccggattgagatacagttacgaagtt5220
actcctagaaccaaaaagatttcaagactactcaagtttgatccagtcaagttgccactg5280
ctacctggcggaattgcaattagaaatttcaaatacatgggacaagtactggatatcata5340
atagacgataacaatggtacaatagctcataagggtggggataagccaattagaatcaag5400
gttccaaatagagatattctccacgacagaaatatcacttccgcactatactcaaagaga5460
gatgacgatttatcagcgacagatgactactacggtacatacttcacactatacccaaat5520
gaggaactggtgattcctctttacgatactaagttaaacatcgacggtaacatagccgaa5580
tctaaacaaataactaatttgactgccggagttccaggtgacgttggtttctccgcatta5640
gatgggaacaattacacacactggcagccattcgataaatcagataatgctaagttgcta5700
attgacttaggttttaacagcactcatgtcattaaaaagggtattattttgtggggtcaa5760
agaccagcgaaaaacatttctttgtcagttttgccacattcagagagaattgagcaacta5820
tttgcgaacatcacagatcttttagaaacatcatctataactaagggcggctctttgtta5880
aaccagatgttaggccaaacacaatcaaacgtgaccgcagaaattgatgatgatatcctc5940
gctttactgaactggaaaggtgacgatctggaccaactcatcccttatttgcctgatatg6000
catctcctacaagagaaattcattcctattttgaaggattaccctattaagccaaatcag6060
agatactataaggaaatcattgacgatgacataatcaagttattaccatcaaacacaact6120
gaatttacaatcgattacaattctatccctggcggggaaaagcgtgcaagatacgttgtt6180
ctgacagttcacggcacctatgatgacgatgatgatcttaaaggtgcaacaatcaaggaa6240
attgttctgcaggaatgattaattaaacaggccccttttcctttgtcgatatcatgtaat6300
tagttatgtcacgcttacattcacgccctcctcccacatccgctctaaccgaaaaggaag6360
gagttagacaacctgaagtctaggtccctatttatttttttatagttatgttagtattaa6420
gaacgttatttatatttcaaatttttcttttttttctgtacaaacgcgtgtacgcatgta6480
acgggcagacgcggccgccaccgcggtggagctccaattcgccctatagtgagtcgtatt6540
acaattcactggccgtcgttttacaacgtcgtgactgggaaaaccctggcgttacccaac6600
ttaatcgccttgcagcacatccccccttcgccagctggcgtaatagcgaagaggcccgca6660
ccgatcgcccttcccaacagttgcgcagcctgaatggcgaatggcgcgacgcgccctgta6720
gcggcgcattaagcgcggcgggtgtggtggttacgcgcagcgtgaccgctacacttgcca6780
gcgccctagcgcccgctcctttcgctttcttcccttcctttctcgccacgttcgccggct6840
ttccccgtcaagctctaaatcgggggctccctttagggttccgatttagtgctttacggc6900
acctcgaccccaaaaaacttgattagggtgatggttcacgtagtgggccatcgccctgat6960
agacggtttttcgccctttgacgttggagtccacgttctttaatagtggactcttgttcc7020
aaactggaacaacactcaaccctatctcggtctattcttttgatttataagggattttgc7080
cgatttcggcctattggttaaaaaatgagctgatttaacaaaaatttaacgcgaatttta7140
acaaaatattaacgtttacaatttcctgatgcggtattttctccttacgcatctgtgcgg7200
tatttcacaccgcagggtaataactgatataattaaattgaagctctaatttgtgagttt7260
agtatacatgcatttacttataatacagttttttagttttgctggccgcatcttctcaaa7320
tatgcttcccagcctgcttttctgtaacgttcaccctctaccttagcatcccttcccttt7380
gcaaatagtcctcttccaacaataataatgtcagatcctgtagagaccacatcatccacg7440
gttctatactgttgacccaatgcgtctcccttgtcatctaaacccacaccgggtgtcata7500
atcaaccaatcgtaaccttcatctcttccacccatgtctctttgagcaataaagccgata7560
acaaaatctttgtcgctcttcgcaatgtcaacagtacccttagtatattctccagtagat7620
agggagcccttgcatgacaattctgctaacatcaaaaggcctctaggttcctttgttact7680
tcttctgccgcctgcttcaaaccgctaacaatacctgggcccaccacaccgtgtgcattc7740
gtaatgtctgcccattctgctattctgtatacacccgcagagtactgcaatttgactgta7800
ttaccaatgtcagcaaattttctgtcttcgaagagtaaaaaattgtacttggcggataat7860
gcctttagcggcttaactgtgccctccatggaaaaatcagtcaagatatccacatgtgtt7920
tttagtaaacaaattttgggacctaatgcttcaactaactccagtaattccttggtggta7980
cgaacatccaatgaagcacacaagtttgtttgcttttcgtgcatgatattaaatagcttg8040
gcagcaacaggactaggatgagtagcagcacgttccttatatgtagctttcgacatgatt8100
tatcttcgtttcctgcaggtttttgttctgtgcagttgggttaagaatactgggcaattt8160
catgtttcttcaacactacatatgcgtatatataccaatctaagtctgtgctccttcctt8220
cgttcttccttctgttcggagattaccgaatcaaaaaaatttcaaagaaaccgaaatcaa8280
aaaaaagaataaaaaaaaaatgatgaattgaattgaaaagcgtggtgcactctcagtaca8340
atctgctctgatgccgcatagttaagccagccccgacacccgccaacacccgctgacgcg8400
ccctgacgggcttgtctgctcccggcatccgcttacagacaagctgtgaccgtctccggg8460
agctgcatgtgtcagaggttttcaccgtcatcaccgaaacgcgcgagacgaaagggcctc8520
gtgatacgcctatttttataggttaatgtcatgataataatggtttcttaggacggatcg8580
cttgcctgtaacttacacgcgcctcgtatcttttaatgatggaataatttgggaatttac8640
tctgtgtttatttatttttatgttttgtatttggattttagaaagtaaataaagaaggta8700
gaagagttacggaatgaagaaaaaaaaataaacaaaggtttaaaaaatttcaacaaaaag8760
cgtactttacatatatatttattagacaagaaaagcagattaaatagatatacattcgat8820
taacgataagtaaaatgtaaaatcacaggattttcgtgtgtggtcttctacacagacaag8880
atgaaacaattcggcattaatacctgagagcaggaagagcaagataaaaggtagtatttg8940
ttggcgatccccctagagtcttttacatcttcggaaaacaaaaactattttttctttaat9000
ttctttttttactttctatttttaatttatatatttatattaaaaaatttaaattataat9060
tatttttatagcacgtgatgaaaaggacccaggtggcacttttcggggaaatgtgcgcgg9120
aacccctatt9130
<210>14
<211>1128
<212>prt
<213>人工序列(artificialsequence)
<220>
<223>由多种生物体光滑假丝酵母和酿酒酵母共同组成的序列
<400>14
metlyspheileserthrpheleuthrpheileleualaalavalser
151015
valthralaserthrasnsertyrpheserserserleualaserala
202530
serproleupheasnlysgluglyargvalvalargprometargglu
354045
serasnleuglyleuhisalaaspproglnthrarglysserserlys
505560
thrleutyraspleuleuserasppheaspasnalaphetyraspasp
65707580
gluasnmetileleuglyserleualapheglygluasnthrtyrser
859095
argglnprotyrvalalaasnglytyrileglyserargileproasn
100105110
ileglypheglytyralaleuaspthrleuasnleutyralaaspala
115120125
proglyalaleuasnasnglytrpproleuargasnargargpheala
130135140
glyserphevalseraspphetyrserleuglnalalysleuasnser
145150155160
thrasnpheprogluleuaspglulysglytyrthrthrvalileser
165170175
serileproglutrpthraspleuglnphethrvalaspleuasngly
180185190
thrasntrppheasnproglnservalleuileaspaspvalileasn
195200205
tyrasnglnasnleusermetlysaspglyilevalserthrasnmet
210215220
asptrpleuasnglymetileasnilelyssergluvaltrpalahis
225230235240
arglysilehisserleuglyilethrargleugluileserleuasn
245250255
leuaspalaleuproaspgluphethrgluleuprovalthriletyr
260265270
aspileileasppheasnthrserhisargthrthrleutyrglulys
275280285
glyglnaspgluaspasnlysalailetyrmetilevalasnproglu
290295300
asnvalprotyrserasnalavalvaltyrserthrcysthrilelys
305310315320
glythrgluasnasnpheserprotyrasnphethrseraspasparg
325330335
ilealaargasntyrmetthrasnleuthrglugluasnprolysval
340345350
valiletyrlystyrthrservalvalserserglutyrasnasnasp
355360365
gluproasnproasnvalasnleulysphealaserasnilealaasn
370375380
thralalysglyasntyrlysserleuleuserasnhislysargala
385390395400
trptyraspleutyrasnaspalapheilegluileproseraspser
405410415
leuleuglumetthralaargserserleuphehisleuleualaasn
420425430
thrargglntyrasnvalserthrthrargglyleuprovalglyval
435440445
glyglyleuserseraspsertyrglyglymetvalphetrpaspala
450455460
aspvaltrpmetalaproalaleuleuprophepheproasnileala
465470475480
metasnmetasnasntyrargasnalathrhisglnglnalaileglu
485490495
asnalalysglntyrasntyrproglyalavaltyrprotrpthrser
500505510
glyargtyralaasncysthrserthrglyprocysileasptyrglu
515520525
tyrhisileasnvalaspilealaleualaserpheseriletyrmet
530535540
asnglyalagluglyalaaspgluasptyrleuargphethrthrtrp
545550555560
prometvallysaspalaalavalphephelysalatyrvallystyr
565570575
asngluthrleuglyglutyrgluthrtyrasnleuthraspproasp
580585590
gluphealaasnhisvalasnasnglyalaphethrasnalaglyile
595600605
lysthrleuleulystrpalathraspileglythrhisleuglyglu
610615620
gluvalaspprolystrpmetgluilealaaspasnilehisilepro
625630635640
argseraspserasnilethrleuglutyrserglymetasnserser
645650655
valgluilelysglnalaaspvalthrleumetvaltyrproleugly
660665670
tyrileasnaspgluserileleuasnasnalailelysaspleutyr
675680685
tyrtyrsergluargglnseralaserglyproalametthrtyrpro
690695700
valphevalalaalaalaalaserleuleuasnhisglyserserser
705710715720
glnsertyrleutyrlysservalleuprotyrleuargserprophe
725730735
alaglnphesergluglnseraspaspasnpheleuthrasnglyleu
740745750
thrglnproalaphepropheleuthralaasnglyglypheleugln
755760765
serileleupheglyleuthrglyleuargtyrsertyrgluvalthr
770775780
proargthrlyslysileserargleuleulyspheaspprovallys
785790795800
leuproleuleuproglyglyilealaileargasnphelystyrmet
805810815
glyglnvalleuaspileileileaspaspasnasnglythrileala
820825830
hislysglyglyasplysproileargilelysvalproasnargasp
835840845
ileleuhisaspargasnilethrseralaleutyrserlysargasp
850855860
aspaspleuseralathraspasptyrtyrglythrtyrphethrleu
865870875880
tyrproasnglugluleuvalileproleutyraspthrlysleuasn
885890895
ileaspglyasnilealagluserlysglnilethrasnleuthrala
900905910
glyvalproglyaspvalglypheseralaleuaspglyasnasntyr
915920925
thrhistrpglnpropheasplysseraspasnalalysleuleuile
930935940
aspleuglypheasnserthrhisvalilelyslysglyileileleu
945950955960
trpglyglnargproalalysasnileserleuservalleuprohis
965970975
sergluargilegluglnleuphealaasnilethraspleuleuglu
980985990
thrserserilethrlysglyglyserleuleuasnglnmetleugly
99510001005
glnthrglnserasnvalthralagluileaspaspaspileleu
101010151020
alaleuleuasntrplysglyaspaspleuaspglnleuilepro
102510301035
tyrleuproaspmethisleuleuglnglulyspheileproile
104010451050
leulysasptyrproilelysproasnglnargtyrtyrlysglu
105510601065
ileileaspaspaspileilelysleuleuproserasnthrthr
107010751080
gluphethrileasptyrasnserileproglyglyglulysarg
108510901095
alaargtyrvalvalleuthrvalhisglythrtyraspaspasp
110011051110
aspaspleulysglyalathrilelysgluilevalleuglnglu
111511201125
<210>15
<211>9382
<212>dna
<213>人工序列(artificialsequence)
<220>
<223>由多种生物体光滑假丝酵母和酿酒酵母共同组成的序列
<400>15
aaatctttgtcgctcttcgcaatgtcaacagtacccttagtatattctccagtagatagg60
gagcccttgcatgacaattctgctaacatcaaaaggcctctaggttcctttgttacttct120
tctgccgcctgcttcaaaccgctaacaatacctgggcccaccacaccgtgtgcattcgta180
atgtctgcccattctgctattctgtatacacccgcagagtactgcaatttgactgtatta240
ccaatgtcagcaaattttctgtcttcgaagagtaaaaaattgtacttggcggataatgcc300
tttagcggcttaactgtgccctccatggaaaaatcagtcaagatatccacatgtgttttt360
agtaaacaaattttgggacctaatgcttcaactaactccagtaattccttggtggtacga420
acatccaatgaagcacacaagtttgtttgcttttcgtgcatgatattaaatagcttggca480
gcaacaggactaggatgagtagcagcacgttccttatatgtagctttcgacatgatttat540
cttcgtttcctgcaggtttttgttctgtgcagttgggttaagaatactgggcaatttcat600
gtttcttcaacactacatatgcgtatatataccaatctaagtctgtgctccttccttcgt660
tcttccttctgttcggagattaccgaatcaaaaaaatttcaaagaaaccgaaatcaaaaa720
aaagaataaaaaaaaaatgatgaattgaattgaaaagcgtggtgcactctcagtacaatc780
tgctctgatgccgcatagttaagccagccccgacacccgccaacacccgctgacgcgccc840
tgacgggcttgtctgctcccggcatccgcttacagacaagctgtgaccgtctccgggagc900
tgcatgtgtcagaggttttcaccgtcatcaccgaaacgcgcgagacgaaagggcctcgtg960
atacgcctatttttataggttaatgtcatgataataatggtttcttaggacggatcgctt1020
gcctgtaacttacacgcgcctcgtatcttttaatgatggaataatttgggaatttactct1080
gtgtttatttatttttatgttttgtatttggattttagaaagtaaataaagaaggtagaa1140
gagttacggaatgaagaaaaaaaaataaacaaaggtttaaaaaatttcaacaaaaagcgt1200
actttacatatatatttattagacaagaaaagcagattaaatagatatacattcgattaa1260
cgataagtaaaatgtaaaatcacaggattttcgtgtgtggtcttctacacagacaagatg1320
aaacaattcggcattaatacctgagagcaggaagagcaagataaaaggtagtatttgttg1380
gcgatccccctagagtcttttacatcttcggaaaacaaaaactattttttctttaatttc1440
tttttttactttctatttttaatttatatatttatattaaaaaatttaaattataattat1500
ttttatagcacgtgatgaaaaggacccaggtggcacttttcggggaaatgtgcgcggaac1560
ccctatttgtttatttttctaaatacattcaaatatgtatccgctcatgagacaataacc1620
ctgataaatgcttcaataatattgaaaaaggaagagtatgagtattcaacatttccgtgt1680
cgcccttattcccttttttgcggcattttgccttcctgtttttgctcacccagaaacgct1740
ggtgaaagtaaaagatgctgaagatcagttgggtgcacgagtgggttacatcgaactgga1800
tctcaacagcggtaagatccttgagagttttcgccccgaagaacgttttccaatgatgag1860
cacttttaaagttctgctatgtggcgcggtattatcccgtattgacgccgggcaagagca1920
actcggtcgccgcatacactattctcagaatgacttggttgagtactcaccagtcacaga1980
aaagcatcttacggatggcatgacagtaagagaattatgcagtgctgccataaccatgag2040
tgataacactgcggccaacttacttctgacaacgatcggaggaccgaaggagctaaccgc2100
tttttttcacaacatgggggatcatgtaactcgccttgatcgttgggaaccggagctgaa2160
tgaagccataccaaacgacgagcgtgacaccacgatgcctgtagcaatggcaacaacgtt2220
gcgcaaactattaactggcgaactacttactctagcttcccggcaacaattaatagactg2280
gatggaggcggataaagttgcaggaccacttctgcgctcggcccttccggctggctggtt2340
tattgctgataaatctggagccggtgagcgtgggtctcgcggtatcattgcagcactggg2400
gccagatggtaagccctcccgtatcgtagttatctacacgacgggcagtcaggcaactat2460
ggatgaacgaaatagacagatcgctgagataggtgcctcactgattaagcattggtaact2520
gtcagaccaagtttactcatatatactttagattgatttaaaacttcatttttaatttaa2580
aaggatctaggtgaagatcctttttgataatctcatgaccaaaatcccttaacgtgagtt2640
ttcgttccactgagcgtcagaccccgtagaaaagatcaaaggatcttcttgagatccttt2700
ttttctgcgcgtaatctgctgcttgcaaacaaaaaaaccaccgctaccagcggtggtttg2760
tttgccggatcaagagctaccaactctttttccgaaggtaactggcttcagcagagcgca2820
gataccaaatactgtccttctagtgtagccgtagttaggccaccacttcaagaactctgt2880
agcaccgcctacatacctcgctctgctaatcctgttaccagtggctgctgccagtggcga2940
taagtcgtgtcttaccgggttggactcaagacgatagttaccggataaggcgcagcggtc3000
gggctgaacggggggttcgtgcacacagcccagcttggagcgaacgacctacaccgaact3060
gagatacctacagcgtgagcattgagaaagcgccacgcttcccgaagggagaaaggcgga3120
caggtatccggtaagcggcagggtcggaacaggagagcgcacgagggagcttccaggggg3180
gaacgcctggtatctttatagtcctgtcgggtttcgccacctctgacttgagcgtcgatt3240
tttgtgatgctcgtcaggggggccgagcctatggaaaaacgccagcaacgcggccttttt3300
acggttcctggccttttgctggccttttgctcacatgttctttcctgcgttatcccctga3360
ttctgtggataaccgtattaccgcctttgagtgagctgataccgctcgccgcagccgaac3420
gaccgagcgcagcgagtcagtgagcgaggaagcggaagagcgcccaatacgcaaaccgcc3480
tctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaa3540
agcgggcagtgagcgcaacgcaattaatgtgagttacctcactcattaggcaccccaggc3600
tttacactttatgcttccggctcctatgttgtgtggaattgtgagcggataacaatttca3660
cacaggaaacagctatgaccatgattacgccaagctcggaattaaccctcactaaaggga3720
acaaaagctgggtaccgggccccccctcgagatctcccgagtttatcattatcaatactg3780
ccatttcaaagaatacgtaaataattaatagtagtgattttcctaactttatttagtcaa3840
aaaattggccttttaattctgctgtaacccgtacatgcccaaaatagggggcgggttaca3900
cagaatatataacatcataggtgtctgggtgaacagtttattcctggcatccactaaata3960
taatggagcccgctttttttaagctggcatccagaaaaaaaaagaatcccagcaccaaaa4020
tattgttttcttcaccaaccatcagttcataggtccattctcttagcgcaactacacaga4080
acaggggcacaaacaggcaaaaaacgggcacaacctcaatggagtgatgcaacctgcttg4140
gagtaaatgatgacacaaggcaattgacctacgcatgtatctatctcattttcttacacc4200
ttctattaccttctgctctctctgatttggaaaaagctgaaaaaaaaggttgaaaccagt4260
tccctgaaattattcccctatttgactaataagtatataaagacggtaggtattgattgt4320
aattctgtaaatctatttcttaaacttcttaaattctacttttatagttagtcttttttt4380
tagttttaaaacactaagaacttagtttcgaataaacacacataaaaacaaatctagaat4440
gggtttcaaggacaagattctgttctggaaagacgaagttcaatatagaacacttgcagt4500
tgctgatcaagtggccaacagattctttcatagcttcgaaaatgtatacaagggtgatga4560
gtctgtcgaagatgcggattcaagacctgttggtttgaccaatgaaaccctatcacattc4620
atctgatttctttgttttgccagaagagagaatctctactagagtcaagattagacgtca4680
gaatatcctaaacacaacactcattttggggatgctgatcgcacttgtaatatggactgc4740
cattttgtctacaaactcttacttctcttcctccttagcttctgcttcaccattattcaa4800
caaagagggcagagtggttagaccaatgcgtgaaagtaacttaggcctacacgcagatcc4860
tcaaactagaaaatccagtaagacactttacgatttgttgtcagactttgacaatgcatt4920
ttacgatgatgaaaacatgatcttaggatccctagccttcggtgagaatacctactccag4980
acagccatacgtcgcaaacgggtacataggtagccgtataccaaacatcggtttcggata5040
cgcacttgatacacttaacttgtacgctgatgcaccaggcgcacttaacaacggttggcc5100
tttgagaaacagaagattcgctggaagtttcgtttctgatttctactcactgcaagccaa5160
gttgaacagtaccaattttcctgagttagatgaaaagggttatacaactgtcatttcatc5220
aatcccagaatggacagatttgcaattcactgtagaccttaacggcactaactggttcaa5280
tccacaatcagttctaatcgatgatgttattaattacaatcagaacttgagcatgaagga5340
tggaattgttagcacaaacatggattggttgaacggaatgataaacattaagtctgaagt5400
ctgggcacacagaaagatccattccttgggtatcacacgtctggaaatctcattgaacct5460
tgatgctttacctgacgagttcactgaactaccagttacaatttacgatatcatagattt5520
caacacttctcatagaacaacactatatgagaaaggtcaagatgaagataacaaagcgat5580
ttacatgattgtaaacccagaaaacgtaccatactcaaacgcagttgtgtactctacttg5640
tacaatcaagggcactgaaaacaacttttctccttacaacttcacatcagatgatagaat5700
tgcgagaaactacatgacaaacctaacagaagagaacccaaaagtcgttatttacaagta5760
cacatcagtggtttcctcagaatataataatgacgagccaaatcctaacgttaacttgaa5820
gttcgcctcaaacatcgctaacaccgcaaaaggaaactataagtctttattgtccaatca5880
caaaagagcttggtacgatttgtacaatgatgcctttatcgaaattccatctgatagttt5940
attggaaatgaccgcaagatctagtctcttccatttgcttgcgaacacacgtcaatacaa6000
cgtttctacaactagaggattaccagtcggcgttggtggtctttcttcagactcttacgg6060
ggggatggtcttttgggacgcagacgtatggatggctccagctctcctacctttctttcc6120
aaacatagcaatgaacatgaataactacagaaacgctactcatcaacaagccattgaaaa6180
tgctaagcaatacaattacccaggcgccgtttacccttggacatctggtagatatgcgaa6240
ttgtacctctaccggtccttgcatcgactacgaataccatattaatgtagatatcgcttt6300
ggcaagtttctctatttacatgaacggtgccgagggtgccgacgaagattaccttagatt6360
cacaacatggccaatggtcaaggatgctgcagtgttcttcaaggcctacgtcaaatacaa6420
tgaaacacttggagagtacgaaacttataatttaactgacccagatgaatttgctaatca6480
tgtgaataatggagctttcacaaatgcaggaatcaaaactttgttgaaatgggcaacaga6540
cattggtactcaccttggggaggaagttgatcctaagtggatggaaattgctgataacat6600
acatattcctagaagtgactctaacattactttggagtactctggcatgaatagctccgt6660
tgagattaagcaggctgacgttacattgatggtataccctttaggttacatcaacgatga6720
atctatactaaacaacgcaatcaaggatttgtattactactctgaacgtcagtctgcctc6780
tgggccagctatgacttacccagtgttcgtcgcagctgccgcttccctcttgaaccacgg6840
ttcatcatcacagagttacctttacaaatctgttcttccatatctgagatcaccatttgc6900
tcaatttagcgaacaatcagatgacaatttcttaactaacggtttgacacaaccagcatt6960
cccatttttgaccgctaatggtggtttcttgcaatctatattatttggattaaccggatt7020
gagatacagttacgaagttactcctagaaccaaaaagatttcaagactactcaagtttga7080
tccagtcaagttgccactgctacctggcggaattgcaattagaaatttcaaatacatggg7140
acaagtactggatatcataatagacgataacaatggtacaatagctcataagggtgggga7200
taagccaattagaatcaaggttccaaatagagatattctccacgacagaaatatcacttc7260
cgcactatactcaaagagagatgacgatttatcagcgacagatgactactacggtacata7320
cttcacactatacccaaatgaggaactggtgattcctctttacgatactaagttaaacat7380
cgacggtaacatagccgaatctaaacaaataactaatttgactgccggagttccaggtga7440
cgttggtttctccgcattagatgggaacaattacacacactggcagccattcgataaatc7500
agataatgctaagttgctaattgacttaggttttaacagcactcatgtcattaaaaaggg7560
tattattttgtggggtcaaagaccagcgaaaaacatttctttgtcagttttgccacattc7620
agagagaattgagcaactatttgcgaacatcacagatcttttagaaacatcatctataac7680
taagggcggctctttgttaaaccagatgttaggccaaacacaatcaaacgtgaccgcaga7740
aattgatgatgatatcctcgctttactgaactggaaaggtgacgatctggaccaactcat7800
cccttatttgcctgatatgcatctcctacaagagaaattcattcctattttgaaggatta7860
ccctattaagccaaatcagagatactataaggaaatcattgacgatgacataatcaagtt7920
attaccatcaaacacaactgaatttacaatcgattacaattctatccctggcggggaaaa7980
gcgtgcaagatacgttgttctgacagttcacggcacctatgatgacgatgatgatcttaa8040
aggtgcaacaatcaaggaaattgttctgcaggaatgattaattaaacaggccccttttcc8100
tttgtcgatatcatgtaattagttatgtcacgcttacattcacgccctcctcccacatcc8160
gctctaaccgaaaaggaaggagttagacaacctgaagtctaggtccctatttattttttt8220
atagttatgttagtattaagaacgttatttatatttcaaatttttcttttttttctgtac8280
aaacgcgtgtacgcatgtaacgggcagacgcggccgccaccgcggtggagctccaattcg8340
ccctatagtgagtcgtattacaattcactggccgtcgttttacaacgtcgtgactgggaa8400
aaccctggcgttacccaacttaatcgccttgcagcacatccccccttcgccagctggcgt8460
aatagcgaagaggcccgcaccgatcgcccttcccaacagttgcgcagcctgaatggcgaa8520
tggcgcgacgcgccctgtagcggcgcattaagcgcggcgggtgtggtggttacgcgcagc8580
gtgaccgctacacttgccagcgccctagcgcccgctcctttcgctttcttcccttccttt8640
ctcgccacgttcgccggctttccccgtcaagctctaaatcgggggctccctttagggttc8700
cgatttagtgctttacggcacctcgaccccaaaaaacttgattagggtgatggttcacgt8760
agtgggccatcgccctgatagacggtttttcgccctttgacgttggagtccacgttcttt8820
aatagtggactcttgttccaaactggaacaacactcaaccctatctcggtctattctttt8880
gatttataagggattttgccgatttcggcctattggttaaaaaatgagctgatttaacaa8940
aaatttaacgcgaattttaacaaaatattaacgtttacaatttcctgatgcggtattttc9000
tccttacgcatctgtgcggtatttcacaccgcagggtaataactgatataattaaattga9060
agctctaatttgtgagtttagtatacatgcatttacttataatacagttttttagttttg9120
ctggccgcatcttctcaaatatgcttcccagcctgcttttctgtaacgttcaccctctac9180
cttagcatcccttccctttgcaaatagtcctcttccaacaataataatgtcagatcctgt9240
agagaccacatcatccacggttctatactgttgacccaatgcgtctcccttgtcatctaa9300
acccacaccgggtgtcataatcaaccaatcgtaaccttcatctcttccacccatgtctct9360
ttgagcaataaagccgataaca9382
<210>16
<211>515
<212>prt
<213>扣囊复膜酵母菌(saccharomycopsisfibuligera)
<400>16
metileargleuthrvalpheleuthralavalphealaalavalala
151015
sercysvalprovalgluleuasplysargasnthrglyhisphegln
202530
alatyrserglytyrthrvalalaargserasnphethrglntrpile
354045
hisgluglnproalavalsertrptyrtyrleuleuglnasnileasp
505560
tyrprogluglyglnphelysseralalysproglyvalvalvalala
65707580
serproserthrsergluproasptyrphetyrglntrpthrargasp
859095
thralailethrpheleuserleuilealagluvalgluasphisser
100105110
pheserasnthrthrleualalysvalvalglutyrtyrileserasn
115120125
thrtyrthrleuglnargvalserasnproserglyasnpheaspser
130135140
proasnhisaspglyleuglygluprolyspheasnvalaspaspthr
145150155160
alatyrthralasertrpglyargproglnasnaspglyproalaleu
165170175
argalatyralaileserargtyrleuasnalavalalalyshisasn
180185190
asnglylysleuleuleualaglyglnasnglyileprotyrserser
195200205
alaseraspiletyrtrplysileilelysproaspleuglnhisval
210215220
serthrhistrpserthrserglypheaspleutrpglugluasngln
225230235240
glythrhisphephethralaleuvalglnleulysalaleusertyr
245250255
glyileproleuserlysthrtyrasnaspproglyphethrsertrp
260265270
leuglulysglnlysaspalaleuasnsertyrileasnsersergly
275280285
phevalasnserglylyslyshisilevalgluserproglnleuser
290295300
serargglyglyleuaspseralathrtyrilealaalaleuilethr
305310315320
hisaspileglyaspaspaspthrtyrthrpropheasnvalaspasn
325330335
sertyrvalleuasnserleutyrtyrleuleuvalaspasnlysasn
340345350
argtyrlysileasnglyasntyrlysalaglyalaalavalglyarg
355360365
tyrprogluaspvaltyrasnglyvalglythrsergluglyasnpro
370375380
trpglnleualathralatyralaglyglnthrphetyrthrleuala
385390395400
tyrasnserleulysasnlyslysasnleuvalileglulysleuasn
405410415
tyraspleutyrasnserpheilealaaspleuserlysileaspser
420425430
sertyralaserlysaspserleuthrleuthrtyrglyseraspasn
435440445
tyrlysasnvalilelysserleuleuglnpheglyaspserpheleu
450455460
lysvalleuleuasphisileaspaspasnglyglnleuthrgluglu
465470475480
ileasnargtyrthrglypheglnalaglyalavalserleuthrtrp
485490495
serserglyserleuleuseralaasnargalaargasnlysleuile
500505510
gluleuleu
515
<210>17
<211>579
<212>prt
<213>米根霉(rhizopusoryzae)
<400>17
alaserileproserseralaservalglnleuaspsertyrasntyr
151015
aspglyserthrpheserglylysiletyrvallysasnilealatyr
202530
serlyslysvalthrvalvaltyralaaspglyseraspasntrpasn
354045
asnasnglyasnthrilealaalaserpheserglyproilesergly
505560
serasntyrglutyrtrpthrpheseralaservallysglyilelys
65707580
gluphetyrilelystyrgluvalserglylysthrtyrtyraspasn
859095
asnasnseralaasntyrglnvalserthrserlysprothrthrthr
100105110
thralaalathrthrthrthrthralaproserthrserthrthrthr
115120125
argprosersersergluproalathrpheprothrglyasnserthr
130135140
ilesersertrpilelyslysglngluaspileserargphealamet
145150155160
leuargasnileasnproproglyseralathrglypheilealaala
165170175
serleuserthralaglyproasptyrtyrtyralatrpthrargasp
180185190
alaalaleuthrserasnvalilevaltyrglutyrasnthrthrleu
195200205
serglyasnlysthrileleuasnvalleulysasptyrvalthrphe
210215220
servallysthrglnserthrserthrvalcysasncysleuglyglu
225230235240
prolyspheasnproaspglyserglytyrthrglyalatrpglyarg
245250255
proglnasnaspglyproalagluargalathrthrphevalleuphe
260265270
alaaspsertyrleuthrglnthrlysaspalasertyrvalthrgly
275280285
thrleulysproalailephelysaspleuasptyrvalvalasnval
290295300
trpserasnglycyspheaspleutrpglugluvalasnglyvalhis
305310315320
phetyrthrleumetvalmetarglysglyleuleuleuglyalaasp
325330335
phealalysargasnglyaspserthrargalaserthrtyrserser
340345350
thralaserthrilealaasnlysileserserphetrpvalserser
355360365
asnasntrpvalglnvalserglnservalthrglyglyvalserlys
370375380
lysglyleuaspvalserthrleuleualaalaasnleuglyserval
385390395400
aspaspglyphephethrproglyserglulysileleualathrala
405410415
valalavalgluaspserphealaserleutyrproileasnlysasn
420425430
leuprosertyrleuglyasnalaileglyargtyrprogluaspthr
435440445
tyrasnglyasnglyasnserglnglyasnprotrppheleualaval
450455460
thrglytyralagluleutyrtyrargalailelysglutrpileser
465470475480
asnglyglyvalthrvalserserileserleuprophephelyslys
485490495
pheaspserseralathrserglylyslystyrthrvalglythrser
500505510
asppheasnasnleualaglnasnilealaleualaalaaspargphe
515520525
leuserthrvalglnleuhisalaproasnasnglyserleualaglu
530535540
glupheaspargthrthrglypheserthrglyalaargaspleuthr
545550555560
trpserhisalaserleuilethralasertyralalysalaglyala
565570575
proalaala
<210>18
<211>621
<212>prt
<213>白宇佐美曲霉(aspergillusshirousami)
<400>18
servalileserlysargalathrleuaspsertrpleuserasnglu
151015
alathrvalalaargthralaileleuasnasnileglyalaaspgly
202530
alatrpvalserglyalaaspserglyilevalvalalaserproser
354045
thraspasnproasptyrphetyrthrtrpthrargaspserglyile
505560
valleulysthrleuvalaspleupheargasnglyaspthraspleu
65707580
leuserthrilegluhistyrileserserglnalaileileglngly
859095
valserasnproserglyaspleuserserglyglyleuglyglupro
100105110
lyspheasnvalaspgluthralatyralaglysertrpglyargpro
115120125
glnargaspglyproalaleuargalathralametileglyphegly
130135140
glntrpleuleuaspasnglytyrthrseralaalathrgluileval
145150155160
trpproleuvalargasnaspleusertyrvalalaglntyrtrpasn
165170175
glnthrglytyraspleutrpglugluvalasnglyserserphephe
180185190
thrilealavalglnhisargalaleuvalgluglyseralapheala
195200205
thralavalglysersercyssertrpcysaspserglnalaprogln
210215220
ileleucystyrleuglnserphetrpthrglysertyrileleuala
225230235240
asnpheaspserserargserglylysaspthrasnthrleuleugly
245250255
serilehisthrpheaspproglualaglycysaspaspserthrphe
260265270
glnprocysserproargalaleualaasnhislysgluvalvalasp
275280285
serpheargseriletyrthrleuasnaspglyleuseraspserglu
290295300
alavalalavalglyargtyrprogluaspsertyrtyrasnglyasn
305310315320
protrppheleucysthrleualaalaalagluglnleutyraspala
325330335
leutyrglntrpasplysglnglyserleugluilethraspvalser
340345350
leuaspphephelysalaleutyrserglyalaalathrglythrtyr
355360365
serserserserserthrtyrserserilevalseralavallysthr
370375380
phealaaspglyphevalserilevalgluthrhisalaalaserasn
385390395400
glyserleusergluglnpheasplysseraspglyaspgluleuser
405410415
alaargaspleuthrtrpsertyralaalaleuleuthralaasnasn
420425430
argargasnservalvalproprosertrpglygluthrseralaser
435440445
servalproglythrcysalaalathrseralaserglythrtyrser
450455460
servalthrvalthrsertrpproserilevalalathrglyglythr
465470475480
thrthrthralathrthrthrglyserglyglyvalthrserthrser
485490495
lysthrthrthrthralaserlysthrserthrthrthrserserthr
500505510
sercysthrthrprothralavalalavalthrpheaspleuthrala
515520525
thrthrthrtyrglygluasniletyrleuvalglyserilesergln
530535540
leuglyasptrpgluthrseraspglyilealaleuseralaasplys
545550555560
tyrthrserserasnproprotrptyrvalthrvalthrleuproala
565570575
glygluserpheglutyrlyspheileargvalgluseraspaspser
580585590
valglutrpgluseraspproasnargglutyrthrvalproglnala
595600605
cysglygluserthralathrvalthraspthrtrparg
610615620
<210>19
<211>616
<212>prt
<213>土曲霉(aspergillusterreus)
<400>19
alaproglnleualaproargalathrthrserleuaspalatrpleu
151015
alasergluthrthrvalalaleuaspglyileleuaspasnvalgly
202530
serserglyalatyralalysseralalysserglyilevalileala
354045
serproserthrseraspproasptyrtyrtyrthrtrpthrargasp
505560
alaalaleuthrvallysalaleuileaspleupheargasnglyglu
65707580
thrserleuglnthrvalilemetglutyrileserserglnalatyr
859095
leuglnthrvalserasnproserglyserleuserthrglyglyleu
100105110
alagluprolystyrtyrvalaspgluthralatyrthrglysertrp
115120125
glyargproglnargaspglyproalaleuargalathralametile
130135140
asppheglyasntrpleuileaspasnglytyrserthrtyralaser
145150155160
serilevaltrpproilevalargasnaspleusertyrvalalagln
165170175
tyrtrpasnglnthrglytyraspleutrpglugluvalasnglyser
180185190
serphephethrilealavalglnhisargalaleuvalgluglyser
195200205
thrphealaserlysvalglyalasercyssertrpcysaspsergln
210215220
alaproglnvalleucyspheleuglnargphetrpthrglysertyr
225230235240
ilemetalaasnpheglyglyglyargserglylysaspalaasnthr
245250255
valleuglyserilehisthrpheaspproasnalaglycysaspasp
260265270
thrthrpheglnprocysserproargalaleualaasnhislysval
275280285
tyrthraspserpheargseriletyrserileasnserglyileser
290295300
serglylysalavalalavalglyargtyrprogluaspsertyrtyr
305310315320
asnglyasnprotrppheleuthrthrleualaalaalagluglnleu
325330335
tyraspalailetyrglntrpglnlysileglyserilethrilethr
340345350
aspvalserleualaphephelysaspleutyrserseralaalaval
355360365
glythrtyralaserserserseralaphethrserilevalserala
370375380
vallysthrtyralaaspglytyrmetserilevalglnthrhisala
385390395400
metthrasnglyserleusergluglnpheglylysseraspglyphe
405410415
serleuseralaargaspleuthrtrpsertyralaalaleuleuthr
420425430
alaasnleuargargasnservalvalproprosertrpglygluthr
435440445
thralathrservalproservalcysseralathrseralathrgly
450455460
thrtyrserthralathrasnthralatrpproserthrleuthrser
465470475480
glythrglyalathrthrthrthrserlysalathrserserserthr
485490495
thrthrthrserseralaserserthrthrvalglucysvalvalpro
500505510
thralavalalavalthrpheaspgluvalalathrthrthrtyrgly
515520525
gluasnvaltyrvalvalglyserileserglnleuglysertrpasp
530535540
thrserlysalavalalaleuseralaserlystyrthrserserasn
545550555560
asnleutrptyrvalthrvalthrleuproalaglythrthrphegln
565570575
tyrlyspheileargvalserserserglyservalthrtrpgluser
580585590
aspproasnargsertyrthrvalproseralacysglythrserthr
595600605
alavalvalasnthrthrtrparg
610615
<210>20
<211>617
<212>prt
<213>米卡塔酵母(saccharomycesmikatae)
<400>20
metlysasnpheileserleuvalasnlyslyslysglythrleuasp
151015
aspargasnserservalprogluserserserglyileilehisgln
202530
argglyalaleuasnthrgluasppheglugluglylyslysaspgly
354045
alaphegluleuglyhisleugluphethrthrasnseralaglnleu
505560
glyaspseraspaspaspasnaspasnalailelysilealaasnala
65707580
alathraspglualaasnglualaasnsergluglulyssermetthr
859095
leuargglnalaleuarglystyrprolysalaalaleutrpserile
100105110
leuvalserthrthrleuvalmetgluglytyraspthralaleuleu
115120125
seralaleutyralaleuprovalpheglnarglyspheglythrmet
130135140
asnalagluglysertyrgluilethrserglntrpglnileglyleu
145150155160
asnmetcysvalleucysglyglumetileglyleuglnmetthrthr
165170175
tyrmetvalgluphemetglyasnargtyrthrmetilethralaleu
180185190
glyleuleuthralatyrilepheileleutyrtyrcyslysserleu
195200205
alametilealavalglyglnileleuseralametprotrpglycys
210215220
pheglnserleualavalthrtyralasergluvalcysproleuala
225230235240
leuargtyrtyrmetthrsertyrserasnilecystrpleuphegly
245250255
glnilephealaserglyilemetlysasnserglngluasnleugly
260265270
aspseraspleuglytyrlysleuprophealaleuglntrpiletrp
275280285
proalaproleuileileglyilephephealaprogluserprotrp
290295300
trpleuvalarglysasnlysilealaglualalyslysserleuasn
305310315320
argileleuserglythralaalagluarggluileglnvalaspile
325330335
thrleulysglnileglumetthrileglulysgluargleuleuala
340345350
serlysserglyserphepheasncysphelysglyvalaspglyarg
355360365
argthrargleualacysleuthrtrpvalalaglnasnsersergly
370375380
alavalleuleuglytyrserthrtyrphephegluargalaglymet
385390395400
alathrasplysalaphethrpheserleuileglntyrcysleugly
405410415
leualaglythrleucyssertrpvalileserglyargvalglyarg
420425430
trpserileleualatyrglyleualapheglnmetvalcysleuphe
435440445
ileileglyglymetglyphealaserglyserasnalaserasngly
450455460
alaglyglyleuleuleualaleuserphephetyrasnalaglyile
465470475480
glyalavalvaltyrcysilevalalagluileproseralagluleu
485490495
argthrlysthrilevalmetalaargilecystyrasnleumetala
500505510
valileasnalaileleuthrprotyrmetleuasnvalserasptrp
515520525
asntrpglyalalysthrglyleutyrtrpglyglyphethralaval
530535540
thrleualatrpvalileileaspleuprogluthrthrglyargthr
545550555560
phesergluileasngluleupheasnglnglyvalproalaarglys
565570575
phealaserthrvalvalasppropheglylysglyglnargglnasn
580585590
aspserglnvalaspasnvalileaspglnserserseralametgln
595600605
glngluleuasnglualaasngluphe
610615
<210>21
<211>1928
<212>dna
<213>酿酒酵母(saccharomycescerevisiae)
<400>21
caagctataccaagcatacaatcaactatctcatatacaatgaaaaacatcatttcatta60
gtttctaagaagaaggctgcatccaaaaatgaagataaaaacatctctgaatcttcaaga120
gatatcgttaatcaacaagaggttttcaataccgaagatttcgaggaaggcaaaaaggac180
tctgcattcgaattagatcacttggagtttactaccaactccgcacaattaggcgactct240
gatgaggacaacgaaaacgtcattaacgaaatgaacgcaacagacgatgcaaacgaagca300
aactctgaagagaaatccatgaccttgaagcaagcattgttgaagtatcctaaagccgca360
ttatggtctattttagtctccactactttggtcatggaaggttacgacactgctttgtta420
tccgcattatacgctttaccagtttttcaacgtaagtttggtacattgaatggtgaaggt480
tcctatgagattacatcccaatggcagattggtcttaacatgtgcgtcttgtgtggtgaa540
atgattggtttacaaatcaccacctatatggttgagtttatgggtaacagatacactatg600
atcaccgctttaggtcttttgactgcttacattttcattttgtactattgtaagtcctta660
gccatgattgctgttggtcaaattttgtccgccatcccttggggttgttttcaatctttg720
gctgttacctatgcttctgaagtctgcccattagcacttagatactatatgacttcctat780
tctaacatttgctggttgttcggccaaatcttcgcatctggtatcatgaaaaactcacaa840
gagaacttaggcaattccgatcttggttacaaacttccatttgctttacagtggatttgg900
cctgccccacttatgattggtattttctttgctccagaatctccttggtggcttgttaga960
aaagatagagttgcagaagctagaaagtcattatccagaattttgtctggtaagggcgct1020
gaaaaggatattcaagttgatcttaccttaaagcagattgaattaactattgaaaaggaa1080
agattattagcttctaagtctggttcattctttaactgtttcaagggtgttaatggtcgt1140
agaaccagattagcatgtttaacctgggttgcccaaaactcctccggtgcagttttgtta1200
ggttattctacttactttttcgaaagagctggtatggcaacagacaaggctttcacattc1260
tcattaatccagtactgtttgggtcttgcaggcaccttatgttcatgggttatttcaggt1320
agagtcggtagatggactatcttgacttatggtcttgcttttcaaatggtctgtttgttc1380
attatcggtggtatgggttttggttctggttcctctgcttccaatggtgctggtggctta1440
ttgttggcattatcctttttctacaatgcaggcatcggtgctgttgtctattgtattgtc1500
gccgaaattccttcagcagaattgagaaccaagactattgttttggctagaatctgttac1560
aatttgatggccgttatcaatgctattttgaccccatacatgttgaacgtttctgactgg1620
aactggggtgctaagacaggcttgtattggggtggtttcactgcagttactttggcttgg1680
gttattatcgacttaccagaaaccactggtagaacattctctgaaatcaatgaattattc1740
aaccaaggtgttccagctcgtaagttcgcatctactgtcgtcgatccattcggtaagggt1800
aagacccaacatgattccttggccgatgaatccatctctcaatcttcatccattaagcaa1860
agagaattgaatgcagcagataagtgttaattaattaaacaggccccttttcctttgtcg1920
atatcatg1928
<210>22
<211>523
<212>prt
<213>东方伊萨酵母(issatchenkiaorientalis)
<400>22
metseraspleugluserglnhisthrargglugluleuasplysser
151015
gluaspglugluileglnleuileasngluserargproprolysleu
202530
asplysgluthrilelysargtyrleulysthrargphethrgluleu
354045
leuprothrlysglnglnmetlysglnasnlyshisleuleuasnpro
505560
leuproglyleuargmetmetglyargargglntrpleumetpheleu
65707580
seralaphecysalatrpthrtrpaspalatyraspphephethrval
859095
serleuasnthrvalglnleualalysasppheasplysasnvallys
100105110
aspilethrtrpglyilethrleuvalleumetleuargservalgly
115120125
glyilethrpheglytyrleuglyaspargtyrglyarglystrppro
130135140
leuvalalaasnleuleuilevalcysileleugluileglysergly
145150155160
pheilelysthrtyrargglnpheleuglyvalargalaleuphegly
165170175
ilecysleuglyglyvaltyrglyasnalathralathralaleuasp
180185190
aspcysprothrglualaargglypheileglyglyleuleuglngln
195200205
glytyralapheglytyrleuleualavalvalphelysargalaile
210215220
alaaspasnserserhislystrpargalathrphetrppheglyala
225230235240
glyileserpheleuphealaleupheargalaserleuprogluthr
245250255
lysalapheleuarglyslysgluilegluargtyrasnlysgluhis
260265270
glyiletyrglnprothrpheglnmetlysilevalgluserleulys
275280285
asntyrtrpleumetmetiletyrleuvalleuphemetserglyphe
290295300
serphemetserhisserserglnaspleutyrprothrleuleuthr
305310315320
valargtyrserphesergluasnlysalathrvalthrasncysval
325330335
alaasnileglyalapheileglyglyvalphethrglyhisileser
340345350
asnpheleuglyargargleuthrilemetleuilecysilephegly
355360365
glyalaleuiletyrprotrpalapheileaspglythrglyileasn
370375380
alaglyvalphepheleuglntrpcysvalglnglyglyleuglyval
385390395400
valproserhisleusergluleualaproproaspleuargalaphe
405410415
valvalglyilesertyrglnleuglyasnleualaalaseralaser
420425430
serthrilegluthrthrileglygluglnpheprometthrserpro
435440445
serglygluproiletyrasptyralalysvalmetalailepheval
450455460
glycysvalphealaphevalleuilevalthrleupheglyproglu
465470475480
argargasnalaserphegluasnalavalthrthrasnglugluphe
485490495
glyasnseralaglyalavalasplysilegluhisvalgluglucys
500505510
ilevalprolyshisalaargasnglnglncys
515520
<210>23
<211>616
<212>prt
<213>酿酒酵母(saccharomycescerevisiae)
<400>23
metserserserilethraspglulysileserglygluglnglngln
151015
proalaglyarglysleutyrtyrasnthrserthrphealaglupro
202530
proleuvalaspglygluglyasnproileasntyrgluprogluval
354045
tyrasnproasphisglulysleutyrhisasnproserleuproala
505560
glnserileglnaspthrargaspaspgluleuleugluargvaltyr
65707580
serglnaspglnglyvalglutyrglugluaspglugluasplyspro
859095
asnleuseralaalaserilelyssertyralaleuthrargphethr
100105110
serleuleuhisilehisgluphesertrpgluasnvalasnproile
115120125
progluleuarglysmetthrtrpglnasntrpasntyrphephemet
130135140
glytyrphealatrpleuseralaalatrpalaphephecysvalser
145150155160
valservalalaproleualagluleutyraspargprothrlysasp
165170175
ilethrtrpglyleuglyleuvalleuphevalargseralaglyala
180185190
valilepheglyleutrpthrasplysserserarglystrpprotyr
195200205
ilethrcysleupheleuphevalilealaglnleucysthrprotrp
210215220
cysaspthrtyrglulyspheleuglyvalargtrpilethrglyile
225230235240
alametglyglyiletyrglycysalaseralathralailegluasp
245250255
alaprovallysalaargserpheleuserglyleuphepheserala
260265270
tyralametglypheilephealaileilephetyrargalaphegly
275280285
tyrpheargaspaspglytrplysileleuphetrppheserilephe
290295300
leuproileleuleuilephetrpargleuleutrpprogluthrlys
305310315320
tyrphethrlysvalleulysalaarglysleuileleuseraspala
325330335
vallysalaasnglyglygluproleuprolysalaasnphelysgln
340345350
lysmetvalsermetlysargthrvalglnlystyrtrpleuleuphe
355360365
alatyrleuvalvalleuleuvalglyproasntyrleuthrhisala
370375380
serglnaspleuleuprothrmetleuargalaglnleuglyleuser
385390395400
lysaspalavalthrvalilevalvalvalthrasnileglyalaile
405410415
cysglyglymetilepheglyglnphemetgluvalthrglyargarg
420425430
leuglyleuleuilealacysthrmetglyglycysphethrtyrpro
435440445
alaphemetleuargserglulysalaileleuglyalaglyphemet
450455460
leutyrphecysvalpheglyvaltrpglyileleuproilehisleu
465470475480
alagluleualaproalaaspalaargalaleuvalalaglyleuser
485490495
tyrglnleuglyasnleualaseralaalaalaserthrilegluthr
500505510
glnleualaaspargtyrproleugluargaspalaserglyalaval
515520525
ilelysgluasptyralalysvalmetalaileleuthrglyserval
530535540
pheilephethrphealacysvalphevalglyhisglulysphehis
545550555560
argaspleuserserprovalmetlyslystyrileasnglnvalglu
565570575
glutyrglualaaspglyleuserileseraspilevalgluglnlys
580585590
thrglucysalaservallysmetileaspserasnvalserlysthr
595600605
tyrglugluhisilegluthrval
610615
<210>24
<211>600
<212>prt
<213>乳酸克鲁维酵母(kluyveromyceslactis)
<400>24
metasnasnasnasnilethrprogluseraspsermetlysserasn
151015
aspasnaspglnthrasnasptyrmetproaspvalalaasppheasp
202530
histhrglnthrasnthrasngluilealaargalaileserhispro
354045
glyservalleuserargvalalasertyrvalserarglysasparg
505560
tyrvalaspgluasnglyasngluvaltrpglnaspaspgluvalser
65707580
ileleumetglugluaspgluthrproaspphethrtrplysasnile
859095
arghistyralailethrargphethrthrleuthrgluleuhisarg
100105110
valsermetgluasnileasnproileprogluleuarglysmetthr
115120125
leuhisasntrpasntyrphephemetglytyralaalatrpleucys
130135140
alaalatrpalaphephealavalservalserthralaproleuala
145150155160
thrleutyrglylysgluthrlysaspilesertrpglyleuserleu
165170175
valleuphevalargseralaglyalaileilepheglyiletrpthr
180185190
aspasntyrserarglystrpprotyrilethrcysleuglyleuphe
195200205
leuilecysglnleucysthrprotrpalalysthrtyrthrglnphe
210215220
leuglyvalargtrpileserglyilealametglyglyiletyrala
225230235240
cysalaseralathralailegluaspalaprovallysalaargser
245250255
pheleuserglyleuphephethralatyralametglypheilephe
260265270
alaileilephetyrargalapheleuasnvalasnglygluasntyr
275280285
trplysvalglnphetrppheseriletrpleuproalavalleuile
290295300
leutrpargleuvaltrpprogluthrlystyrphethrlysvalleu
305310315320
lysalaargglnleumetargaspaspalailealalysasnglygly
325330335
glnproleuprolysleuserphelysglnlysphealaasnvallys
340345350
lysthrvalserlystyrtrpleuleupheglytyrleuileleuleu
355360365
leuvalglyproasntyrleuthrhisalaserglnaspleuphepro
370375380
thrmetleuargalaglnleuargphesergluaspalavalthrval
385390395400
alailevalvalvalcysleuglyserilealaglyglymetphephe
405410415
glyglnleumetgluilethrglyargargvalglyleuleuleuala
420425430
leuilemetalaglycysphethrtyrproalaphemetleulysthr
435440445
serseralavalleuglyalaglyphemetleutrppheserileleu
450455460
glyvaltrpglyvalleuproilehisleusergluleuserpropro
465470475480
glualaargalaleuvalserglyleualatyrglnleuglyasnleu
485490495
alaseralaalaservalvalilegluasnaspleualaaspleutyr
500505510
proileglutrpasnseralaglygluvalthrasnlysasptyrser
515520525
lysvalmetalaileleuthrglyserservalilephethrpheval
530535540
leuvalphevalglyhisglulysphehisargaspleuserserpro
545550555560
hisleulyssertyrilegluargvalaspglnthrglugluvalala
565570575
alametthrglyserthralaasnserileserserlysproserasp
580585590
aspglnleuglulysvalserval
595600
<210>25
<211>606
<212>prt
<213>多布克鲁维酵母(kluyveromycesdobzhanskii)
<400>25
methisthrileserprogluseraspserleulysserserlysser
151015
asnthrasnserhisglutyrmetseralathrthrasptyrasnasn
202530
glyglyalaseralaalaproleuseralalysarglysgluthrgly
354045
asnsertyrgluleuglnasphisalaalaseralaseralaserala
505560
seralaseralaseralaseralaseralaseralaleualapheasp
65707580
tyraspprogluaspaspaspvalserilemetmetglnaspgluglu
859095
valproaspleuthrtrplyshisvalserasntyralailethrarg
100105110
phethrserleualahisilehisprovalsermetglnasnileasn
115120125
propheprogluleuarglysmetthrleuhisasntrpasntyrphe
130135140
phemetglytyrglyalatrpleucysalasertrpalaphepheala
145150155160
valservalserthralaproleualagluleutyrasplysglnthr
165170175
lysaspilesertrpglyleuserleuvalleuphevalargserala
180185190
glyalaileilepheglyiletrpthraspasntyrserarglystrp
195200205
protyrilethrcysleuglyleupheleuilecysglnleucysthr
210215220
prophealathrasntyrthrglnpheleuglyvalargtrpileser
225230235240
glyvalalametglyglyiletyralacysalaseralathralaile
245250255
gluaspalaprovallysalaargserpheleuserglyleuphephe
260265270
thralatyralametglypheilephealathrvalphetyrargala
275280285
pheleuasnileasnglylysasptyrtrplysileglnphetrpphe
290295300
seriletrpleuproalaalaleuileleutrpargleuvaltrppro
305310315320
gluthrargtyrphethrlysvalleulysalaargglnleumetleu
325330335
aspaspalailealaalaasnglyglyglnproleuprolyslysthr
340345350
pheglnglulysvalthrasnvallyslysileileserthrtyrtrp
355360365
leuleuphethrtyrleuileleuleuleuvalglyproasntyrleu
370375380
thrhisalasermetaspleupheprothrmetleuargalaglnleu
385390395400
lysphesergluaspalavalthrvalalailevalvalvalcysile
405410415
glyalailecysglyglymetphepheglyglnleumetgluilethr
420425430
glyargargleuglyleuleuilealaleuvalmetalaglycysphe
435440445
thrtyrproalaphemetleuasnthrglyproalavalleuglygly
450455460
glyphepheleutrppheservalileglyvaltrpglyvalilepro
465470475480
ilehisleusergluleuserproproglualaargalaleuvalser
485490495
glyleualatyrglnleuglyasnleualaseralaalaservalval
500505510
ilegluseraspleualaaspleutyrproilealatrpasnalaala
515520525
glygluvalthrasnlysasntyrserlysvalmetalaileleuthr
530535540
glyservalvalilephethrphevalleuvalleuvalglyhisglu
545550555560
lysphehisargaspleuserserprohisleulysglutyrthrasp
565570575
argvalaspargalagluglualaalaglymetglyserseralaala
580585590
servalserserlysprogluglnasnphevalglulysval
595600605
<210>26
<211>604
<212>prt
<213>马克斯克鲁维酵母(kluyveromycesmarxianus)
<400>26
metsergluglyasngluglulysglyglnglyglnglyglnglygln
151015
asphisglyhispheprogluglyargthrpheleuhisglnvalglu
202530
serlysargservalhisthrhishisservalprometasnserser
354045
aspserglnserasnalaseraspproglugluleualaprophepro
505560
servalvalaspglusergluleuaspglusergluleuglugluile
65707580
glnasngluaspvalserileiletyrlysaspaspgluglulyspro
859095
aspleusertrpalaserilecysargtyralaglythrargilethr
100105110
serleupheglnilehislysvalsermetaspasnileasnprophe
115120125
progluleuarglysmetthrleuserasntrpasntyrphephemet
130135140
glytyrleualatrpleucysalaalatrpalaphephealavalser
145150155160
valserthralaproleualalysleutyrasplysprothrlysasp
165170175
ilesertrpglyleuserleuvalleuphevalargseralaglyala
180185190
ileilepheglyleutrpthraspasntyrserarglystrpprotyr
195200205
ilethrcysleuglyleupheleuilecysglnleucysthrprotrp
210215220
alalysthrtyrthrglnpheleuglyvalargtrpileserglyile
225230235240
alametglyglyiletyrglycysalaseralathralailegluasp
245250255
alaprovallysalaargserileleuserglyleuphepheserala
260265270
tyralametglypheilephealaileilephetyrargalapheleu
275280285
hisvalasnglygluasntyrtrplysvalglnphetrppheserile
290295300
pheleuproalaleuleumetleutrpargleuvaltrpprogluthr
305310315320
lystyrphethrlysvalleulysalaarggluleumetlysglnasp
325330335
alailealaserasnglyglyasnproileprolysthrthrphelys
340345350
glulysmetalaasnthrlysargileilecyslystyrtrpleuleu
355360365
pheglytyrleuvalleuleuleuvalglyproasntyrleuthrhis
370375380
glyserglnaspleupheprothrmetileargserglnleuasnphe
385390395400
sergluaspalavalthrvalalailevalvalvalasnleuglygly
405410415
ilecysglyglyleuphepheglyglnleumetgluvalthrglyarg
420425430
argleuglyleuleuilealaleuthrmetalaglycysphethrtyr
435440445
proalaphemetleulysthrserseralavalleuglyalaglyphe
450455460
metleuphepheservalpheglyvaltrpglyvalileproilehis
465470475480
leusergluleuserproproglualaargalaleuvalalaglyleu
485490495
alatyrglnleuglyasnleualaseralaalaservalvalileglu
500505510
asnaspleualaaspiletyrproleualaargaspalaalaglylys
515520525
valilelyslysasptyralalysvalmetalaileleuthrglyser
530535540
valvalilephethrphevalmetvalleuileglyhisglulysphe
545550555560
hisargaspleuserseralavalleulysalatyrmetglulysval
565570575
aspargasnglugluaspalaargleuglyvalserserleuserile
580585590
serserlysserlysglugluglnvalgluasnala
595600
<210>27
<211>591
<212>prt
<213>酿酒酵母(saccharomycescerevisiae)
<400>27
metleulystyrlysproleuleulysileserlysasncysgluala
151015
alaileleuargalaserlysthrargleuasnthrileargalatyr
202530
glyserthrvalprolysserlysserphegluglnaspserarglys
354045
argthrglnsertrpthralaleuargvalglyalaileleualaala
505560
thrserservalalatyrleuasntrphisasnglyglnileaspasn
65707580
gluprolysleuaspmetasnlysglnlysileserproalagluval
859095
alalyshisasnlysproaspaspcystrpvalvalileasnglytyr
100105110
valtyraspleuthrargpheleuproasnhisproglyglyglnasp
115120125
valilelyspheasnalaglylysaspvalthralailepheglupro
130135140
leuhisalaproasnvalileasplystyrilealaproglulyslys
145150155160
leuglyproleuglnglysermetproprogluleuvalcyspropro
165170175
tyralaproglygluthrlysgluaspilealaarglysgluglnleu
180185190
lysserleuleuproproleuaspasnileileasnleutyraspphe
195200205
glutyrleualaserglnthrleuthrlysglnalatrpalatyrtyr
210215220
serserglyalaasnaspgluvalthrhisarggluasnhisasnala
225230235240
tyrhisargilephephelysprolysileleuvalaspvalarglys
245250255
valaspileserthraspmetleuglyserhisvalaspvalprophe
260265270
tyrvalseralathralaleucyslysleuglyasnproleuglugly
275280285
glulysaspvalalaargglycysglyglnglyvalthrlysvalpro
290295300
glnmetileserthrleualasercysserproglugluileileglu
305310315320
alaalaproserasplysglnileglntrptyrglnleutyrvalasn
325330335
serasparglysilethraspaspleuvallysasnvalglulysleu
340345350
glyvallysalaleuphevalthrvalaspalaproserleuglygln
355360365
argglulysaspmetlysleulyspheserasnthrlysalaglypro
370375380
lysalametlyslysthrasnvalglugluserglnglyalaserarg
385390395400
alaleuserlyspheileaspproserleuthrtrplysaspileglu
405410415
gluleulyslyslysthrlysleuproilevalilelysglyvalgln
420425430
argthrgluaspvalilelysalaalagluileglyvalserglyval
435440445
valleuserasnhisglyglyargglnleuasppheserargalapro
450455460
ilegluvalleualagluthrmetproileleugluglnargasnleu
465470475480
lysasplysleugluvalphevalaspglyglyvalargargglythr
485490495
aspvalleulysalaleucysleuglyalalysglyvalglyleugly
500505510
argpropheleutyralaasnsercystyrglyargasnglyvalglu
515520525
lysalailegluileleuargaspgluileglumetsermetargleu
530535540
leuglyvalthrserilealagluleulysproaspleuleuaspleu
545550555560
serthrleulysalaargthrvalglyvalproasnaspvalleutyr
565570575
asngluvaltyrgluglyprothrleuthrgluphegluaspala
580585590
<210>28
<211>578
<212>prt
<213>东方伊萨酵母(issatchenkiaorientalis)
<400>28
metleuleuargserleuasnserseralaargcysvallysglnthr
151015
thrargthrlysvalargtyrleuserhisvalserglyalasermet
202530
alalysprothrleulysasnasnserarggluserasnlysserarg
354045
asntyrleuilealaalavalthralaleualavalserthrserile
505560
glyvalalavalhisvallysaspproleutyrasnaspalathrgly
65707580
seraspserproargserileservalaspgluphevallyshisasn
859095
serglnasnaspcystrpilealaileasnglylysvaltyraspphe
100105110
thrasppheileproasnhisproglyglyvalproproleuvalasn
115120125
hisalaglytyraspglythrlysleutyrglulysleuhisprolys
130135140
glythrileglulyspheleuprolysasplyspheleuglyvalleu
145150155160
aspglyglualaprolysleuglualaasptyrleuvalaspaspasp
165170175
gluglngluargleuasptyrleuasnasnleuproproleuserser
180185190
ileglnasnvaltyrasppheglutyrleualalyslysileleupro
195200205
lysaspalatrpalatyrtyrsercysglyalaaspaspgluilethr
210215220
metarggluasnhistyralatyrglnargvaltyrpheargproarg
225230235240
ilecysvalaspvallysgluvalaspthrsertyrglumetleugly
245250255
thrlysthrservalprophetyrvalseralathralaleualalys
260265270
leuglyhisproaspglyglucysserilealaargglyalaglylys
275280285
gluglyvalvalglnmetileserthrleusersermetserleuasp
290295300
gluilealaalaalaargileproglyalathrglntrppheglnleu
305310315320
tyrileasngluaspargasnvalalalysglyleuvallyshisala
325330335
gluaspleuglymetlysalailepheilethrvalaspalaproser
340345350
leuglyasnargglulysasplysargleulysphevalasnaspthr
355360365
aspvalaspleuglyaspseralaaspargasnserglyalaserlys
370375380
alaleuserserpheileaspalaservalsertrpasnaspvallys
385390395400
alavallyssertrpthrlysleuprovalleuvallysglyvalgln
405410415
thrvalgluaspvalileglualatyraspalaglycysglnglyval
420425430
valleuserasnhisglyglyargglnleuaspthralapropropro
435440445
ilegluleuleualagluthrvalprothrleulysargleuglylys
450455460
leuargproaspphegluileleuileaspglyglyvallysarggly
465470475480
thraspileleulysalavalalaileglyglyglnaspvalargval
485490495
servalglymetglyargpropheleutyralaasnsercystyrgly
500505510
glualaglyvalarglysleuileglnasnleulysaspgluleuglu
515520525
metaspmetargleuleuglyvalthrlysmetaspglnleuserser
530535540
lyshisvalaspthrlysargleuileglyargaspalaileasntyr
545550555560
leutyraspasnvaltyrserproilegluthrvallyspheasnasn
565570575
gluasp
<210>29
<211>423
<212>prt
<213>克鲁弗酵母(saccharomyceskluyveri)
<400>29
metproglnaspleuvalcysserproleuthrproglygluthrpro
151015
aspaspilevalarglysglugluleuargserargleuproserleu
202530
aspalaleuvalasnleutyrasppheglutyrleualaserglnile
354045
leuthrlysglnalatrpsertyrtyrserseralaalaaspaspglu
505560
valthrhisarggluasnhisalaalatyrhisargilephephelys
65707580
prolysvalleuvalaspvalarggluvalaspthrserthrlysile
859095
leuglyglnaspvalprovalprophetyrvalseralathralaleu
100105110
cyslysleuglyasnprolysgluglyglulysaspvalalaarggly
115120125
cysglyglnglyproilelyscysproglnmetileserthrleuala
130135140
sercysserleuglngluilevalglualaalaproserlysglugln
145150155160
ileglntrppheglnleutyrvalasnthrasparglysilethrasp
165170175
aspleuilelysglnvalglulysleuglyleulysalailepheval
180185190
thrvalaspalaproserleuglyargargglulysaspmetlysval
195200205
lysphethrasnserasnglyalalysalametglulysthrlysval
210215220
lysgluserlysglyalaseralaalaleuserthrpheileasppro
225230235240
alaleusertrpaspaspilevalgluilelyslyslysthrasnleu
245250255
proilevalilelysglyvalglnargvalgluaspvalleulysala
260265270
alagluvalglyalaalaglyvalvalleuserasnhisglyglyarg
275280285
glnleuasppheserargalaproilegluvalleualagluserval
290295300
provalleulysgluargasnleuserasplysleugluilepheile
305310315320
aspglyglyvalargargglyasnaspvalleulysalaleucysleu
325330335
glyalalysglyvalglyleuglyargpropheleutyralaasnser
340345350
cystyrglylysaspglyvalglnlysalaileglumetleuthrglu
355360365
gluileglnmetsermetargleuleuglyvalthrlysilegluasp
370375380
leulysprogluleuleuaspmetserserpheserglyargthrval
385390395400
asnvalproglnaspserleutyrargservaltyrglulysserglu
405410415
thrvalglnpheleuaspglu
420
<210>30
<211>591
<212>prt
<213>贝酵母(saccharomycesbayanus)
<400>30
metleulystyrlysserleuleuargileserargvalsergluala
151015
alaalaleuargthrserargthrargleuasnthrvalargsertyr
202530
serseralavalglylysserlysserpheglulyshisserglylys
354045
argthrglnsertrpthralaleuservalglyalaileleualaala
505560
alaglysermetalatyrvalasntrpglyserglyglnileglyasn
65707580
aspprolysleuaspmetasnlysglnlysileserproalagluval
859095
alalyshisasnseralaaspaspcystrpvalvalileasnglytyr
100105110
valtyraspleuthrargphevalproasnhisproglyglyproasp
115120125
valilelystyrasnalaglyargaspvalthralailepheglupro
130135140
leuhisalaproasnvalileasplystyrilealaproglulyslys
145150155160
leuglyproleuglnglysermetproprogluleuvalcyspropro
165170175
tyralaproglygluthrlysgluaspilealaarglysgluglnleu
180185190
lysserleuleuproproleuaspasnileileasnleutyraspphe
195200205
glutyrleualaserglnthrleuthrargglnalatrpalatyrtyr
210215220
serserglyserasnaspgluvalserhisarggluasnhisasnala
225230235240
tyrhisargilephephelysprolysileleuvalaspvalserlys
245250255
valaspileserthraspmetleuglyserhisvalaspvalprophe
260265270
tyrvalseralathralaleucyslysleuglyasnproleuglugly
275280285
glulysaspilealaargglycysglyglnglyalathrlysvalpro
290295300
glnmetileserthrleualasercysserproglugluileileser
305310315320
alaalaproseraspgluglnileglntrptyrglnleutyrvalasn
325330335
serasparglysilethraspgluleuvallysservalglulysleu
340345350
glyvallysalaleuphevalthrvalaspalaproserleuglygln
355360365
argglulysaspmetlysleulyspheserasnserlysalaglypro
370375380
lysalametlyslysthraspvalglugluserlysglyalaserarg
385390395400
alaleuserlyspheileaspproserleuthrtrplysaspileglu
405410415
gluleulysserlysthrasnleuproilevalilelysglyvalgln
420425430
argthrgluaspvalvallysalaalagluvalglyvalserglyval
435440445
valleuserasnhisglyglyargglnleuasppheserargalapro
450455460
ilegluvalleualaglualametprovalleuglugluarglysleu
465470475480
lysasplysleugluvaltyrileaspglyglyvalargargglythr
485490495
aspileleulysalaleucysleuglyalalysglyvalglyleugly
500505510
argpropheleutyralaasnsercystyrglyargaspglyvalglu
515520525
lysalailegluileleuargaspgluvalglumetsermetargleu
530535540
leuglyvalalaservalalagluleulysprogluleuleuaspleu
545550555560
serthrleulysalaargthrvalalavalproasnaspvalleutyr
565570575
asngluvaltyrgluglyprothrleuthraspphegluaspala
580585590
<210>31
<211>598
<212>prt
<213>鲁氏接合酵母(zygosaccharomycesrouxii)
<400>31
mettrppheargserlysproiletrplysleusergluvalthrala
151015
argserleuargglyileasnthrlysargserphethrtrpasnser
202530
lysglyserasnalaphevalargserserileleulyslysargser
354045
metalathralaleuleuvalthralavalalaalaalaserserval
505560
lysleuserserargproleuaspasnglyprolysvalaspmetser
65707580
lysproalaileserproaspgluvalalalyshisasnserproasp
859095
aspcystrpvalvalileaspglytyrvaltyraspleuthrgluphe
100105110
alaprovalhisproglyglyprothrvalilelysserasnalagly
115120125
lysaspvalseralailepheaspproleuhisalaproaspvalile
130135140
glulyspheileaspproserlysargleuglyproleugluglyasp
145150155160
metprolysgluleuvalcysalaprophethrproglygluthrpro
165170175
aspaspvalglnarglysleulysleuargalaargleuproproleu
180185190
aspalailethrasnleutyrasppheglupheleualaserglnval
195200205
leuthrlysglnalatrpalatyrtyrserserglyalaaspaspglu
210215220
ilethrmetarggluasnhisphealatyrhisargilephephelys
225230235240
prolysvalleuvalasnvalalagluvalaspthrlysthrglumet
245250255
leuglyalaprovalaspvalprophetyrvalthralathralaleu
260265270
cyslysleuglyasnproalagluglyglulysaspilealaarggly
275280285
cysglyserglyglulyslysvalproglnmetvalserthrleuala
290295300
sercysserleuglugluvalvalasnalaglylysgluaspglnile
305310315320
argtrppheglnleutyrmetasngluaspargservalvalaspgln
325330335
metileserseralaglulysleuglytyrlysglyilephevalthr
340345350
valaspalaproglyleuglyasnargglulysaspthrlysvallys
355360365
pheserserglnalaglyproleuservallyslyslysglulysglu
370375380
asplysglylysaspasnglygluserserglyalaserlystyrleu
385390395400
serlyspheileaspproserpheasptrpaspaspleuvalgluval
405410415
lyslyslysthrlysleuproilevalilelysglyvalglnargval
420425430
gluaspvalvallysalaalagluvalglyalaserglyvalvalleu
435440445
serasnhisglyglyargglnleuasppheserargserproileglu
450455460
valleualaglualaglnproileleulysgluargasnphegluasn
465470475480
pheaspvalphevalaspglyglyileargargglythraspvalval
485490495
lysalaleucysleuglyalalysglyvalglyleuglyargprophe
500505510
leutyralaasnservaltyrglylysgluglyvalglnlysalaile
515520525
aspileleuasnphegluvalglumetthrmetargleuleuglyval
530535540
thrserilelysglnleuglyprogluleuileaspthrsercysile
545550555560
lysserargservalglnvalproargasptyrleutyrasplysthr
565570575
tyrmetserseraspleuvalserphemetproproglngluaspglu
580585590
alaaspglygluasnglu
595
<210>32
<211>589
<212>prt
<213>乳酸克鲁维酵母(kluyveromyceslactis)
<400>32
metargseralavalargvalpheglylysasnargvalserglyleu
151015
argphealaserthrserserlysserproilehislysserserala
202530
thrleuargpheglyargseralalysserglytyrseralapheser
354045
pheglylyslysvalilethrglyglyalapheleuvalalaglyser
505560
valglyilealaleuileserglnpheasnaspservalgluasngly
65707580
alalysvalaspmetthrlysprolysvalserprothrgluvalala
859095
lyshisserserprolysaspcystrpvalvalilegluglytyrval
100105110
tyrasnleuthrasppheileseralahisproglyglyproalaile
115120125
ilegluasnasnalaglylysaspvalthrlysilephealaproile
130135140
hisalaproaspvalileglulystyrilealaprogluasnargile
145150155160
glyproleuaspglythrmetproaspaspleuvalcysalaalaleu
165170175
thrproglygluthrprogluaspvalalaarglysgluglnleuarg
180185190
glnserleuproaspileaspserleuvalasniletyrasppheglu
195200205
pheleualaserglnileleuthrlysglnalatrpsertyrtyrser
210215220
seralaalaaspaspgluvalthrhisarggluasnhisalaalatyr
225230235240
hisargilephephelysproargileleuvalasnvallysgluval
245250255
aspthrserthrthrmetleuglyglulysvalglyvalprophetyr
260265270
valseralathralaleucyslysleuglyasnprolysgluglyglu
275280285
lysaspilealaargglycysglygluseraspvallysprovalgln
290295300
metileserthrleualasercysserleuglngluilevalgluala
305310315320
alaproserlysgluglnileglntrppheglnleutyrvalasnser
325330335
asparglysilethrgluaspleuilelysasnvalglulysleugly
340345350
leulysalailephevalthrvalaspalaproserleuglyasnarg
355360365
glulysaspalalysvallysphethrasnserasnglyalalysala
370375380
metglulysserlysvallysgluserlysglyalaserargalaleu
385390395400
serserpheileaspproalaleuasntrpaspaspileilegluphe
405410415
lyslyslysthrlysleuproilevalilelysglyvalglncysval
420425430
gluaspvalleulysalaalagluileglyvalalaglyvalvalleu
435440445
serasnhisglyglyargglnleuasppheserargalaproileglu
450455460
valleualagluthrmetprovalleuargglulyslysleuaspasp
465470475480
lysilegluilepheileaspglyglyvalargargglythraspile
485490495
leulysalaleucysleuglyalalysglyvalglyleuglyargpro
500505510
pheleutyrserasnsercystyrglylysgluglyvallyslysala
515520525
ilegluleuleulysaspgluleuglumetsermetargleuleugly
530535540
valthrserileaspglnleuserglulystyrleuaspleuserthr
545550555560
ilehisglyargthrvalasnvalproargaspasnleutyrargglu
565570575
valtyrvalprohisgluprothrglnpheleugluglu
580585
<210>33
<211>592
<212>prt
<213>多布克鲁维酵母(kluyveromycesdobzhanskii)
<400>33
metargseralavalargvalphearglysasnargpheprothrgly
151015
leuargleuserserthralaserseralaargglnglyilealaleu
202530
servalglylystyrasnlystyrasnlysasnglythrasnglyser
354045
trplysserserarglysserthrthrtyrglyglyalapheleuval
505560
alaseralavalalailealapheserserglnilethrglnserleu
65707580
gluasnglyvallysvalaspmetglylysproservalserproala
859095
gluvalvallyshisserserproaspaspcystrpvalvalileasp
100105110
glytyrvaltyrasnleuthrglupheileglyalahisproglygly
115120125
proalaileilelysasnasnalaglylysaspvalthrlysilephe
130135140
alaproilehisalaproaspvalileglulystyrilealaprolys
145150155160
asnargleuglyproleugluglythrmetprogluaspargilecys
165170175
alaalaleuthrproglygluthralagluaspvalalaarglysglu
180185190
glnleuargglnleuleuproaspvalasnasnleuthrasnleutyr
195200205
asppheglupheleualaserglnileleuthrlysglnalatrpser
210215220
tyrtyrserseralaalaaspaspgluvalthrglnarggluasnhis
225230235240
alaalatyrhisargilephephelysproargileleuvalasnval
245250255
lysgluvalaspthrserthrthrmetleuglyglulysvalglyval
260265270
prophetyrvalseralathralaleucyslysleuglyasnprogln
275280285
gluglyglulysaspilealaargglycysglygluseraspalalys
290295300
provalglnmetileserthrleualasercysserleuglngluile
305310315320
valglualaalaproserlysgluglnileglntrppheglnleutyr
325330335
valasnserasparglysilethraspaspleuvallysasnalaglu
340345350
lysleuglyleulysalailephevalthrvalaspalaproserleu
355360365
glyasnargglulysaspalalysvallysphethrasnserasngly
370375380
alalysalametglulysserasnvalaspgluserlysglyalaser
385390395400
argalaleuserserpheileaspproalaleuasntrpaspaspile
405410415
valgluleulysthrlysthrlysleuproilevalilelysglyval
420425430
glncysvalgluaspvalleulysalaalagluvalglyalaalagly
435440445
valvalleuserasnhisglyglyargglnleuasppheserargala
450455460
proilegluvalleualagluthrmetproalaleuargglulyslys
465470475480
leuaspasplysleugluvalpheileaspglyglyvalargarggly
485490495
thraspileleulysalaleucysleuglyalalysglyvalglyleu
500505510
glyargpropheleutyrserasnsercystyrglylysgluglyval
515520525
lyslysalailegluleuleulysaspgluleuglumetsermetarg
530535540
leuleuglyvalthrserileaspglnleuserglulystyrleuasp
545550555560
leuserthrilehisglyargthrvalservalproargaspasnile
565570575
tyrargaspvaltyrvalalaglngluproserglnphearggluasp
580585590
<210>34
<211>587
<212>prt
<213>酿酒酵母(saccharomycescerevisiae)
<400>34
metleutrplysargthrcysthrargleuilelysproilealagln
151015
proargglyargleuvalargargsercystyrargtyralaserthr
202530
glythrglyserthraspserserserglntrpleulystyrserval
354045
ilealaserseralathrleupheglytyrleuphealalysasnleu
505560
tyrserarggluthrlysgluaspleuileglulysleuglumetval
65707580
lyslysileaspprovalasnserthrleulysleuserserleuasp
859095
serproasptyrleuhisaspproalalysileasplysvalvalglu
100105110
aspleulysglnvalleuglyasnlysprogluasntyrseraspala
115120125
lysseraspleuaspalahisseraspthrtyrpheasnthrhishis
130135140
proserleugluglnargproargileileleupheprohisthrthr
145150155160
glugluvalserlysileleulysilecyshisaspasnasnmetpro
165170175
valvalpropheserglyglythrserleugluglyhispheleupro
180185190
thrargileglyaspthrilethrvalaspleuserlysphemetasn
195200205
asnvalvallyspheasplysleuaspleuaspilethrvalglnala
210215220
glyleuprotrpgluaspleuasnasptyrleuserasphisglyleu
225230235240
metpheglycysaspproglyproglyalaglnileglyglycysile
245250255
alaasnsercysserglythrasnalatyrargtyrglythrmetlys
260265270
gluasnileileasnmetthrilevalleuproaspglythrileval
275280285
lysthrlyslysargproarglysserseralaglytyrasnleuasn
290295300
glyleuphevalglysergluglythrleuglyilevalthrgluala
305310315320
thrvallyscyshisvallysprolysalagluthrvalalavalval
325330335
serpheaspthrilelysaspalaalaalacysalaserasnleuthr
340345350
glnserglyilehisleuasnalametgluleuleuaspgluasnmet
355360365
metlysleuileasnalasergluserthraspargcysasptrpval
370375380
glulysprothrmetphephelysileglyglyargserproasnile
385390395400
valasnalaleuvalaspgluvallysalavalalaglnleuasnhis
405410415
cysasnserpheglnphealalysaspaspaspglulysleugluleu
420425430
trpglualaarglysvalalaleutrpservalleuaspalaasplys
435440445
serlysasplysseralalysiletrpthrthraspvalalavalpro
450455460
valserglnpheasplysvalilehisgluthrlyslysaspmetgln
465470475480
alaserlysleuileasnalailevalglyhisalaglyaspglyasn
485490495
phehisalapheilevaltyrargthrproglugluhisgluthrcys
500505510
serglnleuvalaspargmetvallysargalaleuaspalaglugly
515520525
thrcysthrglygluhisglyvalglyileglylysargglutyrleu
530535540
leuglugluleuglyglualaprovalaspleumetarglysilelys
545550555560
leualaileaspprolysargilemetasnproasplysilephelys
565570575
thraspproasngluproalaasnasptyrarg
580585
<210>35
<211>533
<212>prt
<213>东方伊萨酵母(issatchenkiaorientalis)
<400>35
metserargleuphelysvalleulysalathrglnphelystyrval
151015
serserpheleuleuglyserthrileserglyglyvalvaltyrtyr
202530
asntyrlyslysserleuasnpheserpheproprothrserthrthr
354045
proilethralaalaproproleuglntyrglyaspvalserlysala
505560
ilesergluilethrserlysleuglyproserlysilethrpheser
65707580
glusergluileleuhishisseraspserserpheserthrasphis
859095
alalysprohisgluargprohisalavalvaltyrprogluserthr
100105110
gluaspvalsergluvalleulysilecyshisthrleulysvalpro
115120125
valvalprophethrglyglythrserleugluglyhispheileser
130135140
thrargasnglyileservalaspleuserargmetasnlysvalleu
145150155160
alailehisprogluaspleuaspalavalvalglnproalavalgly
165170175
trpglugluleuargaspthrleuserasptyrasnleuleuphegly
180185190
proaspproglyproglyalacysileglyglymetvalalathrser
195200205
cysserglythrasnalaalaargtyrglythrmetarggluasnval
210215220
valservallysvalvalleuproaspglythrilevallysthrlys
225230235240
argargproarglysserseralaglytyrasnleuthrasnleuphe
245250255
ileglysergluglythrleuglyilevalthrgluvalthrleulys
260265270
leuasnvallysprolystyrgluservalalaleuvalserpheasp
275280285
lysleulysaspalaalaglyservalthrserphevalglnglugly
290295300
leuglnleuasnalailegluleuleuaspasplysmetvalhisphe
305310315320
valasngluserglygluthraspleulystyraspgluleuprothr
325330335
leuleuleulysileglyglyserserprogluservalasnleuleu
340345350
ilelysthrvalgluargilevalasnalaasnasnalalysthrphe
355360365
lysphealaserasnglugluglulystyrglnleutrpasnalaarg
370375380
lysthralaleutrpserthrileglutyrglyargargasnileasp
385390395400
lysaspileglnvaltrpthrthraspvalalavalprometserlys
405410415
phevalglualavalglugluthrglnlysgluileglugluserasp
420425430
leuleualaserilevalglyhisalaglyaspglyasntyrhisthr
435440445
valileleuphelysglugluglnargalaleualaalalysleuval
450455460
serasnmetvalaspargalaleualapheaspglythrvalsergly
465470475480
gluhisglyileglyvalglylyslysglupheleuileaspgluval
485490495
glyglngluservalaspleumetarglysilelyspheserileasp
500505510
prohislysileleuasnproasplysvalpheserileaspproval
515520525
asnaspargserile
530
<210>36
<211>564
<212>prt
<213>克鲁弗酵母(saccharomyceskluyveri)
<400>36
metphelysargalapheleuargproglnleutrpserserthrthr
151015
argargcysthrilealaserileargcystyrserlysglnproleu
202530
lysproalaseralaserlysleuthrleuglyleuileglyglyile
354045
glyleuglyseralaalathralatyrglyleuleumetglyaspasp
505560
aspthrseralaserthrthrargleuaspgluleualaproproser
65707580
tyrcysglnaspprolyslysphegluaspalaileilelysleulys
859095
hisileleuaspaspasnproaspasnpheservalserlysserglu
100105110
leuaspalahisseraspthrtyrpheasnserhishisalaargpro
115120125
aspglnargproglnileileleupheproargthrthrseraspval
130135140
sergluileleulysvalcyshisglutyrgluvalprovalilepro
145150155160
pheserglyglythrserleugluglyhispheleuprothrargile
165170175
glycysthrvalvalleuaspmetserlystyrmetasnlysileleu
180185190
lysleuasnlysglnaspleuaspvalgluvalglnalaglyvalpro
195200205
trpgluaspleuasnasptyrleuasnsergluglyvalleuphegly
210215220
cysaspproglyproglyalaglnileglyglycysilealaasnser
225230235240
cysserglythrasnalatyrargtyrglythrmetlysgluasnile
245250255
valasnilethrvalvalleualaaspglythrvalvallysthrlys
260265270
argargproarglysserseralaglytyrasnleuasnglyleuphe
275280285
valglysergluglythrleuglyvalvalthrglnalathrvallys
290295300
cyshisvalmetprolysilegluthrvalalavalvalserphepro
305310315320
thrvalglyaspalaalasercysserserhisileileglnglugly
325330335
ileglnleuasnalametgluleuleuaspaspasnmetmetlysval
340345350
ileasnglnserglyalathrserlysthrasptrpvalgluserpro
355360365
thrleuphephelysileglyglyargserglnasnasnvallysglu
370375380
leuvalaspgluvalserlysilealaglnlystyrhiscyslyslys
385390395400
phegluphealaserseraspaspglulysleugluleutrpgluala
405410415
arglysvalalaleutrpserthrileasnalaglylysalalysasp
420425430
serasnserasnvaltrpthrthraspvalalavalproleusergln
435440445
phealaprovalileglualathrlysglnglumetasnalasergly
450455460
leuilethrthrleuvalglyhisalaglyaspglyasnphehisala
465470475480
pheilevaltyrasnaspaspglnargaspilealaglulysileval
485490495
aspasnmetvalhisargalaileglualagluglythrcysthrgly
500505510
gluhisglyvalglyileglylysarglyspheleuleuhisgluleu
515520525
glyalagluthrvalaspleumetargglnleulysleualaleuasp
530535540
prolysargileleuasnproasplysilephelysileaspproasn
545550555560
gluhisgluhis
<210>37
<211>530
<212>prt
<213>贝酵母(saccharomycesbayanus)
<400>37
metleuargasnileleuvalargglyalaglylyshisleulystyr
151015
glycysargtyrvallysserseralaleuleuglycysargthrlys
202530
ileasntyrtyrserthrlysileglnthrlysleuthrsergluasn
354045
tyrproaspvalglnargaspalaargphelyslysleuthrserasp
505560
aspleuasntyrpheargserileleusergluglngluvalleugln
65707580
alasergluleugluaspleuserphetyrasngluasptrpmetarg
859095
lystyrlysglyglnserlysleuvalleuargprolysthrvalglu
100105110
lysileserleuileleuasntyrcysasnaspglulysilealaval
115120125
valproglnglyglyasnthrglyleuvalglyglyservalproile
130135140
pheaspgluleuileleuserleualaasnleuasnlysileargasp
145150155160
pheaspprovalserglyileleulyscysaspalaglyvalileleu
165170175
gluasnalaasnasntyrvalmetgluglnasntyrmetpheproleu
180185190
aspleuglyalalysglysercyshisvalglyglyvalvalalathr
195200205
asnalaglyglyleuargleuleuargtyrglyserleuhisglyser
210215220
valleuglyleugluvalvalmetproasnglyglnilevalasnser
225230235240
methissermetarglysaspasnthrglytyraspleulysglnleu
245250255
pheileglysergluglythrileglyileilethrglyvalserval
260265270
leuthrvalprolysprolysalapheasnvalsertyrleualaval
275280285
gluserphegluaspvalglnlysvalphevalargalaargglnglu
290295300
leusergluileleuseralaphegluphemetaspserlyssergln
305310315320
ileleualalysserglnleulysaspalathrpheproleugluasp
325330335
gluhisprophetyrileleuilegluthrserglyserasnlysasp
340345350
hisaspaspserlysleugluthrpheleugluservalmetgluglu
355360365
glyvalvalthraspglyvalvalalaglnaspgluthrgluleugln
370375380
asnleutrplystrpargglumetileproglualaserglnalaasn
385390395400
glyglyvaltyrlystyraspvalserleuproleulysaspleutyr
405410415
serleuvalaspalathrasnalaargleuserglualaglyleuval
420425430
glyaspserprolysprovalvalglyalaileglytyrglyhisval
435440445
glyaspglyasnleuhisleuasnvalalavalargglutyrasnlys
450455460
gluileglulysvalleugluprophevaltyrgluphevalserser
465470475480
lyshisglyservalseralagluhisglyleuglypheglnlyslys
485490495
asntyrileglytyrserlysserproglugluvalthrmetilelys
500505510
aspleulysalahistyraspproasnalaileleuasnprotyrlys
515520525
tyrval
530
<210>38
<211>597
<212>prt
<213>鲁氏接合酵母(zygosaccharomycesrouxii)
<400>38
metvalglyserilevalleuleuglnprotrplysargalavalser
151015
hisalavalargproargthrtyrglntyrargtyrpheargthrthr
202530
alaargileseralaasnasplysproglnserphelysasnglnleu
354045
tyrgluserthrglnglnargleuargarggluargalagluglnglu
505560
argpheserglntyrglnthrglnserproglyglyargtyralaala
65707580
leuthrphealaleuvalphepheserthrglyalatyrtyrleugly
859095
serleulysproalaserleuprothrserserthrthrthrleuphe
100105110
gluilegluproproleuhisasnileserproalaasnleuglnala
115120125
alatrpalaaspphevalgluileleuglylysgluasnvalserthr
130135140
gluhisglyaspleuaspvalhisserglyserasptrpsersertyr
145150155160
thrleulyslysaspgluargpropheleuvalleutyrproserthr
165170175
thrglugluvalserargilemetlysvalcyshisglnargvalile
180185190
provalthrprotyrserglyglythrserleugluglyhispheala
195200205
prothrargglyglyvalcysileasppheargargmetasnargile
210215220
leugluleuhislyslysaspleuaspvalvalvalglnproalaval
225230235240
glytrpgluaspleuasnglugluleuserlysaspglyleuphephe
245250255
proproaspproglyproglyalametileglyglymetvalglythr
260265270
glycysserglythrasnalatyrargtyrglythrmetargglutrp
275280285
valleuserleuthrvalvalleualaaspglythrvalilelysthr
290295300
argglnargproarglysserseralaglytyraspleuthrargleu
305310315320
pheileglysergluglythrleuglyleuvalthrglualathrleu
325330335
lysleuthrvallysprolysserglnservalalavalalaserphe
340345350
proserilehisasnalaalaglucysvalthrargvalvalgluglu
355360365
glyileprovalalaglyvalgluileleuaspaspvalglnmetlys
370375380
cysileasnaspserargthrthrargargglntrplysgluserpro
385390395400
thrleuphephelysphethrglythrprovalglyvallysglugln
405410415
ilegluleuvalarglysilevalserserseralaglyglnserphe
420425430
gluphealaargglygluaspglumetlysgluleutrpseralaarg
435440445
lysglualaleutrpservalmetsermetargargglyprogluasp
450455460
argvaltrpthrthraspvalalavalprometserlysleuproasp
465470475480
ileileglualathrlysglnaspmetthrgluserglyleuleuala
485490495
glyilecysglyhisvalglyaspglyasnphehisalaileileleu
500505510
pheasngluaspglulyslysilealagluglyvalvalhisargmet
515520525
vallysargalavalglumetgluglythrvalthrglygluhisgly
530535540
valglyleuilelysargasptyrleuglnhisgluvalglygluthr
545550555560
thrvalaspthrmetargargilelysmetalaleuaspproleuarg
565570575
leuleuasncysasplysvalvalargvalgluglnprothrthrval
580585590
gluleulyslystrp
595
<210>39
<211>576
<212>prt
<213>乳酸克鲁维酵母(kluyveromyceslactis)
<400>39
metpheargphevalglyargserglyphealaleuargglyserleu
151015
glnleuarglysaspvalleuargserargthrthralavalalalys
202530
arghistyrserserserasnglyasnasnglyglyglypheserser
354045
alaileleuservalleuglyglyserleuileglyglyglypheval
505560
alatyralaleuglyserglnpheglulysglulysservalserasp
65707580
leuserilealaargleugluaspleuaspserproglutyrcysasp
859095
lysgluthrphealalysalaleuvalgluleulysaspvalleuglu
100105110
asnaspprogluasnphethrvalalalysaspaspleuaspalahis
115120125
seraspthrtyrpheasnserhishisalaglualaasnglnargpro
130135140
gluilevalleutyrproargasnthrgluaspvalserlysleuleu
145150155160
lysilecyshislystyrserileprovalilepropheserglygly
165170175
thrserleugluglyhispheleuprothrargproglysercysval
180185190
valleuaspileserlystyrleuasnlysileileglnleuasnlys
195200205
gluaspleuaspvalvalvalglnglyglyvalprotrpglugluleu
210215220
asnglutyrleuasnasphisglyleuleupheglycysaspprogly
225230235240
proglyalaglnilealaglycysilealaasnsercysserglythr
245250255
asnalatyrargtyrglythrmetlysgluasnvalvalasnilethr
260265270
metcysmetalaaspglythrilevallysthrlysargargproarg
275280285
lysserseralaglytyrasnleuasnglyleuileileglyserglu
290295300
glythrleuglyilevalthrglualathrilelyscyshisvalarg
305310315320
serthrphegluthrvalalavalvalpropheprothrvalserasp
325330335
alaalasercysserserhisleuileglnalaglyileglnleuasn
340345350
alametgluleuleuaspaspasnmetmetlysileileasnglnser
355360365
glyalathrserlysaspasntrpvalgluserprothrleuphephe
370375380
lysileglyglyargsergluglnileileglngluvalilelysglu
385390395400
valglulysilealaserglnhisasnasnthrlyspheglupheala
405410415
thraspgluaspserlysleugluleutrpglualaarglysvalala
420425430
leutrpserthrileaspthrglyarglysthrasnproaspalaasn
435440445
iletrpthrthraspvalalavalproileserlysphealaaspval
450455460
ileasnalathrlysgluglumetasnalaserglyleuleuthrser
465470475480
leuvalglyhisalaglyaspglyasnphehisalapheileiletyr
485490495
asnthrgluglnarglysthralagluthrilevalgluasnmetval
500505510
lysargalaileaspalagluglythrcysthrglygluhisglyval
515520525
glyileglylysargasptyrleuleuglugluvalglygluaspthr
530535540
valalavalmetarglysleulysleualaleuaspprolysargile
545550555560
leuasnproasplysilephelysileaspproasnasphisglnhis
565570575
<210>40
<211>582
<212>prt
<213>多布克鲁维酵母(kluyveromycesdobzhanskii)
<400>40
metphelysphevalglyargserglythralaleuargserserleu
151015
glnalaargargasnglyalavaltrplysargglytyralasergly
202530
serglyserglyserglyserglyserglyserserserleuvalleu
354045
glyvalleualaglysermetileglyglyglyilevalalatyrthr
505560
leuglyasnglnpheglulysasplyslysvalserglyserserala
65707580
alaalaalaalaseraspleuserileleulysleugluaspleuglu
859095
serprovaltyrcysaspsergluthrpheserthralavalgluglu
100105110
leulysthrvalleuasnasnaspprogluasnphethrthralalys
115120125
alaaspleuaspserhisseraspthrtyrpheasnserhishisala
130135140
gluproaspglnargprogluilevalleupheproargasnthrglu
145150155160
aspvalserglnleuleulysilecyshislystyrserileproval
165170175
ilepropheserglyglythrserleugluglyhispheleuprothr
180185190
argproglysercysvalvalleuaspileserlystyrleuasnlys
195200205
ileilehisleuasnlysgluaspleuaspvalvalvalglnglygly
210215220
valprotrpgluaspleuasnasptyrleuileaspglnglyleuleu
225230235240
pheglycysaspproglyproglyalaglnilealaglycysileala
245250255
asnsercysserglythrasnalatyrargtyrglythrmetlysglu
260265270
asnvalvalasnilethrmetcysmetalaaspglythrileilelys
275280285
thrlyslysargproarglysseralaalaglytyrasnleuasngly
290295300
leuilevalglysergluglythrleuglyilevalthrglualathr
305310315320
ilelyscyshisvalargserleuphegluthrvalalavalvalpro
325330335
pheprothrvalseraspalaalasercysserserhisleuilegln
340345350
alaglyileglnleuasnalametgluleuleuaspaspasnmetmet
355360365
lysileileasnglnserglyalathrserlysileasptrpvalglu
370375380
serprothrleuphephelysileglyglyargthrglulysserile
385390395400
glnaspvalvalaspgluvalglulysilealaserglnhisasnasn
405410415
tyrlysphegluphealathraspalaaspserlysleugluleutrp
420425430
glualaarglysvalalaleutrpserthrileaspalaglylyslys
435440445
leuaspprolysvalasnvaltrpthrthraspvalalavalproile
450455460
serargphealagluvalileasnalathrlysglnglumetasnser
465470475480
serglyleuleuthrserleuvalglyhisalaglyaspglyasnphe
485490495
hisalapheileiletyrasnsergluglnarglysglnalagluthr
500505510
ilevalgluasnmetvallysargalaileaspalagluglythrcys
515520525
thrglygluhisglyvalglyileglylysargasptyrleuvalglu
530535540
gluleuglygluaspalailealavalmetarglysleulysleuala
545550555560
leuaspprolysargileleuasnproasplysilephelysileasp
565570575
proasnaspasnglnhis
580
<210>41
<211>592
<212>prt
<213>马克斯克鲁维酵母(kluyveromycesmarxianus)
<400>41
metleuproargphevalvalargserglyalaalaglyargasnleu
151015
glypheserphethrarglyscysasphisthrpheleuserlysthr
202530
valargasnaspleuserhisargprotyrserthrglythrasngly
354045
asnglyseralaasnalaglylysserglnserglyleuleuphegly
505560
valpheglyglythrleuileglyglyglyleuvalalatyrpheleu
65707580
glyserlyspheaspglnglnglnserserserglnglnvalserasp
859095
leuserilealaargleugluaspleuaspserprolystyrcysasp
100105110
lyslysthrphealathralavalglugluleulysglnvalleuasp
115120125
asnasnprogluasnpheseraspalalysseraspleuaspserhis
130135140
seraspthrtyrpheasnserhishisalathrprogluglnargpro
145150155160
gluilevalleupheproargasnthrgluaspvalserlysleuleu
165170175
lysilecyshislystyrserileprovalilepropheserglygly
180185190
thrserleugluglyhisphemetprothrargproglysercysval
195200205
valleuaspileserlystyrmetasnglnileileglnleuasnlys
210215220
gluaspleuaspvalvalvalglnglyglyvalprotrpgluaspleu
225230235240
asnasptyrleuasnasphisglyleuleupheglycysaspprogly
245250255
proglyalaglnilealaglycysilealaasnsercysserglythr
260265270
asnalatyrargtyrglythrmetlysgluasnvalvalasnilethr
275280285
metcysleualaaspglythrileilelysthrlysargargproarg
290295300
lysserseralaglytyrasnleuasnglyleuileileglyserglu
305310315320
glythrleuglyilevalthrglualathrilelyscyshisvalarg
325330335
serasnphegluthrvalalavalvalpropheproservalalaasp
340345350
alaalasercysserserhisleuileglnalaglyileglnleuasn
355360365
alametgluleuleuaspaspasnmetmetlysileileasnlysser
370375380
glyalathrserargthrasntrpvalgluserprothrleuphephe
385390395400
lysileglyglyargserglulysalailelysgluvalvallysglu
405410415
valglulysilealaserglnhisasnasnserasnpheglupheala
420425430
seraspglugluthrlysleugluleutrpglualaarglysvalala
435440445
leutrpserthrileaspalaglylyslysleuaspproasnvalasn
450455460
valtrpthrthraspvalalavalproileserlysphealaglnval
465470475480
ileasnaspthrlysgluglumetasnalaserglyleuleuthrser
485490495
leuvalglyhisalaglyaspglyasnphehisalapheileiletyr
500505510
asnalagluglnarglysthralagluthrilevalgluasnmetval
515520525
lysargalaileaspalagluglythrcysthrglygluhisglyval
530535540
glyileglylysargglupheleuvalglugluleuglygluaspthr
545550555560
ilealavalmetarglysleulysleualaleuaspprolysargile
565570575
leuasnproasplysvalphelysileaspproasnasphisglnhis
580585590
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<213>酿酒酵母(saccharomycescerevisiae)
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metlysargileargserleutrppheasnalaglualasertyrser
151015
asnleuasnasnserproserleuargasnlysasnserthrglyasn
202530
asnserargserlysasntyrargserpheserargpheaspleuile
354045
asnserileleuleuleumetmetleupheleuleualailepheval
505560
thralaleutyrleuthrlysserserargleuthrtyrserhisala
65707580
serargalaalaleupheasnproleuglyvalileserproserleu
859095
glyasnhisthrleuasntyraspproglualaarggluserserlys
100105110
lysleutyrgluleuleuserasppheasnthralatyrtyraspasp
115120125
gluasnmetileleuglyserasnleupheserlysasnthrtyrser
130135140
argglnprotyrvalalaasnglytyrileglyserargileproasn
145150155160
ileglypheglytyralaleuaspthrleuasnphetyrthraspala
165170175
proglyalaleuasnasnglytrpproleuargasnhisargpheala
180185190
glyalaphevalseraspphetyrcysleuglnprolysleuasnser
195200205
thrasnpheprogluleuaspaspvalglytyrserthrvalileser
210215220
serileproglntrpthrasnleuglnpheserleuvalasnaspser
225230235240
lystrppheasnproglnasnvalthrleuaspaspvalthrasntyr
245250255
serglnasnleusermetlysaspglyilevalthrthrgluleuasp
260265270
trpleuasnserglnilehisvallyssergluiletrpalahisarg
275280285
hisilehisproleuglyvalvalserleugluileserleuasnthr
290295300
asphisleuproserasppheaspserleuaspvalasniletrpasp
305310315320
ileleuasppheasnthrserhisargthrvalleuhisserthrgly
325330335
thraspglulysasnasnalavalphemetilevalglnproaspasn
340345350
valproserserasncysalailetyrserthrcysthrvallystyr
355360365
gluasnserthrasnproileasnsersergluserphegluglulys
370375380
aspvalserserasniletyrasnvalileleuthrgluaspglnpro
385390395400
lysileilevalhislystyrvalglyilemetserthrglupheasn
405410415
lysasnlysgluglnglnaspasnthrasnileglyleualalysmet
420425430
ilealaleuasnserlysglyasntyrglulysleuleuserserhis
435440445
lysargalatrptyraspleutyrasnaspalapheilegluilepro
450455460
seraspserleuleuglumetthralaargserserleuphehisleu
465470475480
leualaasnthrargasptyrasnvalserseraspargglyleupro
485490495
valglyvalserglyleuserseraspsertyrglyglymetvalphe
500505510
trpaspalaaspiletrpmetgluproalaleuleuprophephepro
515520525
asnvalalaglnasnmetasnasntyrargasnalathrhissergln
530535540
alalysleuasnalaglulystyrglytyrproglyalailetyrpro
545550555560
trpthrserglylystyralaasncysthrserthrglyprocysval
565570575
asptyrglutyrhisileasnvalaspvalalametalaserpheser
580585590
iletyrleuasnglyhisgluglyileaspaspglutyrleuargtyr
595600605
thrthrtrpproileilelysasnalaalaglnphephethralatyr
610615620
vallystyrasnserserleuglyleutyrgluthrtyrasnleuthr
625630635640
aspproaspgluphealaasnhisileasnasnglyalaphethrasn
645650655
alaglyilelysthrleuleulystrpalathraspileglyasnhis
660665670
leuglygluvalvalaspprolystrpsergluileserlysaspile
675680685
tyrileproargserserserasnilethrleuglutyrserglymet
690695700
asnserservalgluilelysglnalaaspvalthrleumetvaltyr
705710715720
proleuglytyrileasnaspgluserileleuasnasnalailelys
725730735
aspleutyrtyrtyrsergluargglnseralaserglyproalamet
740745750
thrtyrprovalphevalalaalaalaalaglyleuleuasnhisgly
755760765
serserserglnsertyrleutyrlysservalleuprotyrleuarg
770775780
alaprophealaglnphesergluglnseraspaspasnpheleuthr
785790795800
asnglyleuthrglnproalaphepropheleuthralaasnglygly
805810815
pheleuglnserileleupheglyleuthrglyileargtyrsertyr
820825830
gluvalaspproaspthrlyslysileasnargleuleuargpheasn
835840845
proilegluleuproleuleuproglyglyilealaileargasnphe
850855860
lystyrmetasnglnvalleuaspileileileaspasphisasngly
865870875880
thrilevalhislysserglyaspvalproilehisilelysilepro
885890895
asnargserleuilehisaspglnaspileasnphetyrasnglyser
900905910
gluasngluarglysproasnleugluargargaspvalaspargval
915920925
glyaspprometargmetaspargtyrglythrtyrtyrleuleulys
930935940
prolysglngluleuthrvalglnleuphelysproglyleuasnala
945950955960
argasnasnilealagluasnlysglnilethrasnleuthralagly
965970975
valproglyaspvalalapheseralaleuaspglyasnasntyrthr
980985990
histrpglnproleuasplysilehisargalalysleuleuileasp
99510001005
leuglyglutyrasnglulysgluilethrlysglymetileleu
101010151020
trpglyglnargproalalysasnileserileserileleupro
102510301035
hisserglulysvalgluasnleuphealaasnvalthrgluile
104010451050
metglnasnserglyasnaspglnleuleuasngluthrilegly
105510601065
glnleuleuaspasnalaglyileprovalgluasnvalileasp
107010751080
pheaspglyilegluglngluaspaspgluserleuaspaspval
108510901095
glnalaleuleuhistrplyslysgluaspleualalysleuile
110011051110
gluglnileproargleuasnpheleulysarglysphevallys
111511201125
ileleuaspasnvalprovalserprosergluprotyrtyrglu
113011351140
alaserargasnglnserleuilegluileleuproserasnarg
114511501155
thrthrphethrileasptyrasplysleuglnvalglyasplys
116011651170
glyasnthrasptrparglysthrargtyrilevalvalalaval
117511801185
glnglyvaltyraspasptyraspaspaspasnlysglyalathr
119011951200
ilelysgluilevalleuasnasp
12051210
<210>43
<211>1186
<212>prt
<213>德尔布有孢圆酵母(torulasporadelbrueckii)
<400>43
metserglyargglnargleuleugluserservalalathrasppro
151015
serasnthrargargserlysthrseralaleutrpphetrpvalleu
202530
ilealasermetvalalaleuthrthrvalasnphepheleualaasn
354045
ilealaserphevalserglyleualaasnleuhisgluproglnval
505560
glyalailelysprothrvalthrhishisthrleuaspcysasnglu
65707580
asnalaarglysserserlyslysleutyrgluleuleutyraspphe
859095
glualaserleutyraspaspgluasnmetileleuglyserthrlys
100105110
phetyrproasnthrtyrserlysglnprotyrvalserasnglytyr
115120125
ileglyserargileproasnileglypheglytyralagluaspthr
130135140
leuasnleutrpthrglyserglyaspgluileproglyalaleuasn
145150155160
asnglytrpproleuargasnargargtyralaglyalaphevalser
165170175
aspphetyrcysleuglngluserleuasnserthrasnpheproglu
180185190
leuaspglulysglytyrthrthrvalileserserileproglntrp
195200205
thrasnleuglnphethrileaspaspaspservaltrppheasnpro
210215220
glnasnvalserleuaspgluvalserasntyrasnglnasnmetser
225230235240
metargasnglyvalvalthrthrglnleuasptrpleuaspglymet
245250255
ileasnvalargsergluilevalthrhislyslyshistyralaleu
260265270
glymetmetserleugluileserleuasnleuaspargleupropro
275280285
gluvalprotyrmetaspvalasnvaltyraspvalleuasppheser
290295300
thrserhisargthrvalleuglnserleuglypheasplysgluasn
305310315320
glnglyilephemetilevalgluprogluasnvalprotyrserasn
325330335
alaalavalpheserserserthralaaspaspseralaasnphethr
340345350
trpsergluglutyrserthraspglulysilevalglnserthrhis
355360365
alaasnleuthrarggluasnprolysilealavallyslystyrval
370375380
glyilemetserthrglupheasnthrasnseralahisasnvalser
385390395400
asnilegluphealalysservalserserargserargglyargtyr
405410415
aspgluleumetserthrasnthrlysalatrpalaalailetyrasn
420425430
aspalapheilegluileproseraspalaleuleuglumetthrala
435440445
argserserleutyrhisleuleualaasnthrargthrpheasnval
450455460
seralaaspargglyleuprovalprovalserglyleuserserasp
465470475480
sertyrglyglymetvalphetrpaspalaaspiletrpmetglnpro
485490495
alaleuleuprophepheproaspilealalysasnmetasnasntyr
500505510
argasnalaserhisglnglnalaargleuasnalaglngluasngly
515520525
tyrproglyalavaltyrprotrpthrserglyargtyralaasncys
530535540
thrserthrglyprocysileasptyrglutyrhisileasnvalasp
545550555560
valalaleualaserpheseriletyrmetasnglyglylysglyval
565570575
aspaspasptyrleuargtyrthrthrtrpprometvalargaspala
580585590
alagluphephethrvaltyrvallyspheasngluthrleuglylys
595600605
tyrgluthrtyrasnleuthraspproaspgluphealaasnhisval
610615620
asnasnglyalaphethrasnalaglyilelysthrleuleulystrp
625630635640
alathraspileglytyrhisleuglyglutyrvalaspprolystrp
645650655
ileaspilealalyslysiletyrileproargsercysserasnile
660665670
thrleuglutyrserglymetasnserservalgluilelysglnala
675680685
aspvalproleumetvaltyrproleuglytyrilethraspgluser
690695700
ileleuasnglyalailelysaspleutyrtyrtyrsergluarggln
705710715720
seralaserglyproalametthrtyrprovalphevalalaalaala
725730735
alaglyleuleuasnhisglyserserserglnsertyrleutyrlys
740745750
servalleuprotyrleuargalaprophealaglnpheserglugln
755760765
seraspaspasnpheleuthrasnglyleuthrglnproalaphepro
770775780
pheleuthralaasnglyglypheleuglnserileleupheglyleu
785790795800
thrglyleuargtyrsertyrgluvalaspserserthrlyslysile
805810815
serargleuleulyspheasnprovalgluleuproleuleuprogly
820825830
glyilealaileargasnphelystyrmetasnglnvalleuaspval
835840845
ileileaspaspasnasnglythrilevalhislysserglyaspgln
850855860
proileargvallysvalproaspargsertyrileargaspglnasp
865870875880
ileserphetyrleuglyglyasnserserglyhisaspaspcysphe
885890895
asnasnpheilegluglyglygluglyaspargprovalseralaphe
900905910
glythrtyrtyrthrileasnproglyglugluleuvalleuproleu
915920925
phelysproglyleuasnvalgluglyasnilealagluserlysgln
930935940
valthrasncysthralaglyvalproglyaspvalpropheserala
945950955960
leuaspglyasnasntyrthrhistrpglnproalatyrlystyrasp
965970975
gluglylysleuleuileaspleuglygluaspasnleuglngluile
980985990
lysserglymetileleutrpglyglnargproalaargasnmetser
99510001005
ileserileleuprohisthrtyrgluvalglulysphevalser
101010151020
asnleuthrglnvalleugluhisseraspleuglnlysileglu
102510301035
glygluaspalasergluvalleugluaspvalglyileaspthr
104010451050
asngluleuargasnpheglucysaspaspaspileargthrmet
105510601065
leuasntrplysthrgluthrleugluserleuvalasphisleu
107010751080
lyslysserlysmetileserarghisphevallysileleuasp
108510901095
asntyrserilegluprosergluprotyrphelysgluvaltyr
110011051110
asnlysserleuilegluileleuproserasnlysthrgluphe
111511201125
serileasptyraspalaleulysleuasnaspthralacystyr
113011351140
serasnaspserileglnasnserthralatrparglysthrarg
114511501155
pheileleuvalserileglnglysertyraspaspaspileasp
116011651170
ilelysglyglythrilelysgluilevalleulysval
117511801185
<210>44
<211>1245
<212>prt
<213>西氏哈萨克酵母(kazachstanianaganishii)
<400>44
metalaglntyrlystrpasnprophesertrplystyrserthrleu
151015
gluasnlyslysgluserasnthrglntyrleugluhissersergly
202530
argglugluasnileproleuasnproasnthrsertyrlysleuasn
354045
lysleuargserileserserglygluthrleuasnserasnasparg
505560
serasnileserserlysarglysglnglnileserileglypheleu
65707580
glyvalmetleualaalaileleuvalsermetvalmetvalleuval
859095
thrthrthrasnmetilemetalaserprophecyslysargphehis
100105110
glyvalthrthrthralagluproserarggluhisileaspphearg
115120125
glnglyvalileglnproalaglygluthrhisgluleuprotyrasn
130135140
alaglualaargargalaserlyslysleutyrgluleuleuserasp
145150155160
pheaspthralaphetyraspsergluasnmetileleuglythrthr
165170175
leuphethrgluasnvaltyrserargglnprohisvalalaasngly
180185190
tyrileglyasnargvalproasnileglypheglyphealatyrasp
195200205
alaleuasnphetrpserasnalaserglyalaleuaspasnglytrp
210215220
proleuargasnglnargphealaglyserpheleuseraspphetyr
225230235240
cysleugluprolysleuasnserthrasnpheprogluleuaspasp
245250255
lysglytyrprothrvalileserserileproglutrpserasnleu
260265270
glnpheserleuasnglysertrppheasnthrserservalhisasn
275280285
gluaspvalleuasntyrasnglnasnvalsermetlysaspglyile
290295300
valthrthrglumetasptrptyrasnglymetvalhisvalargser
305310315320
gluiletrpalahisargargilehisproleualavalmetserleu
325330335
gluvalthrleuasnglugluhisleuproaspasppheaspglnleu
340345350
aspileaspilehisaspileleuasphisalathrsermetargthr
355360365
valleuglnaspleuglnvalaspasplysasnseralailetyrmet
370375380
ilevalglnproaspasnvalproaspserasnalaalailetyrser
385390395400
thrcysilevalalaaspserhisasnvalalaglnasnasnpheglu
405410415
pheglyileasnserlysglualaglylysvalserglnileserarg
420425430
asnileleuserarggluasnproserilethrvalthrlyshisval
435440445
glymetmetserthrgluphelysthrglugluhispheleuhisleu
450455460
serasnleuaspvalalaargasnilealaleuasnalalysglygln
465470475480
tyrgluserleuleuserserhislysargalatrptyraspleutyr
485490495
asnaspalapheilegluileproseraspalaleuleuglumetthr
500505510
alaargserserleutyrglnleuleualaasnthrargthrtyrasn
515520525
valsergluargglyleuprovalglyvalglyglyleuserserasp
530535540
sertyrglyglymetvalphetrpaspalaaspiletrpmetalapro
545550555560
alaleuleuprophepheprogluvalserlysasnmetasnasntyr
565570575
argasnserthrhislysglnalaglnleuasnalaglnglualagly
580585590
tyrproglyalavaltyrprotrpthrserglyargphealaasncys
595600605
thrserthrglyprocysileasptyrglutyrhisileasnvalasp
610615620
valalaleualaserpheseriletyrleuasnglyalaalaglyile
625630635640
aspaspasptyrleuargtyrthrthrtrpprometvalargaspala
645650655
alaglnphephethrserphevallystyrasnalathrpheasparg
660665670
tyrgluthrhisasnleuthraspproaspgluphealaasphisile
675680685
aspasnglyalaphethrasnalaglyilelysthrleuleulystrp
690695700
alathraspileglyasnhisleugluglulysvalaspprolystrp
705710715720
progluileserglulysiletyrileproargserproserasnile
725730735
thrleuglutyrserglymetasnserservalgluilelysglnala
740745750
aspvalthrleumetvaltyrproleuglytyrileasnaspgluser
755760765
ileleumetasnalailelysaspleutyrtyrtyrsergluarggln
770775780
seralaserglyproalametthrtyrprovalphevalalaalaala
785790795800
alaglyleuleuasnhisglyserserserglnsertyrleutyrlys
805810815
servalleuprotyrleuargglnprophealaglnpheserglugln
820825830
seraspaspasnpheleuthrasnglyleuthrglnproalaphepro
835840845
pheleuthralaasnglyglypheleuglnserileleupheglyleu
850855860
thrglyileargtyrsertyraspthraspprogluthrlyslysmet
865870875880
serargvalmetargpheasnproileglnleuproleumetprogly
885890895
glyleualaileargasnphelystyrleuasnglnvalleuaspile
900905910
ileileaspaspasnasnglythrilevalhislysserglyaspgln
915920925
proilethrileleuvalproasnargaspleuilehisaspargasp
930935940
ileasnvaltyrargargasnsersertyralalysargaspleuphe
945950955960
serarggluglumetglnileglulysserglnglnlysasnvalser
965970975
glythrtyrtyrthrleuhisproasnglugluleuvalleuproleu
980985990
phelysproglyleuasnilegluasnasnvalalagluasnglngln
99510001005
ilethrasnleuthralaglyvalproglyaspvalalapheser
101010151020
valleuaspglyasnasntyrthrhistrpglnproleuthrlys
102510301035
alaservalglylysleuleuileaspleuglyargasnasnglu
104010451050
lysaspleuarghisglythrileleutrpglyglnargproala
105510601065
lysasnvalserileserileleuprohisasngluleuileglu
107010751080
gluilepheserasnvalthrtyrileleugluasnserglnasp
108510901095
lysaspprolyslysthrileglugluleuvalglyasntyrval
110011051110
hisvalasnproaspasnlysaspcysvalseraspvalleuthr
111511201125
ileleuasntrplysmetaspasnvalaspgluvalilelysgln
113011351140
metproaspileglyleuilehisgluthrphevalthrileval
114511501155
aspasnleugluilegluproasngluprotyrtyraspgluleu
116011651170
ileargasnserglnilegluleuleuproserasnmetthrval
117511801185
pheaspileasptyrserlysalaasppheserasnthrleulys
119011951200
cyspheasnserglyasngluglnaspmetilelysalaargtyr
120512101215
ilevalvalservalglnglythrtyraspgluaspgluaspthr
122012251230
lysglyalathrilelysgluvalalapheglyval
123512401245
<210>45
<211>1386
<212>prt
<213>蟑螂四盾酵母(tetrapisisporablattae)
<400>45
metgluasnthrargargthraspmetproseraspargaspileser
151015
asnileglnserglyaspasplysserasnileglumetleuaspleu
202530
hishiscysaspaspleuserilethraspasnileglnlysasnasp
354045
leuhisgluglnglyalathrthrserglnproasnargseraspasp
505560
ileglualaasnglutyrthrleupheaspilemetasphisserglu
65707580
gluaspasnasnglyaspserglngluasnseraspaspglngluasn
859095
aspaspgluthralaprophethrhisserglyglyasnasnasnasn
100105110
asngluaspaspaspglyaspaspasnasnasnlyslysilethrasn
115120125
lysasnasnthrasnserileileasnserasnserhisphehisthr
130135140
trpserglyilelysargserileproserleualaleuphealaphe
145150155160
leumetcyscysvalleuileleumetthrserthrleuleuhisgly
165170175
serserthrserpheserthrphelyslysasnmetpheasntrparg
180185190
hisasnasnglyvalalaasnserpheleuargpheasnhispheasn
195200205
asnglyprothrtyrasnilehisasnthrserhisileserleuser
210215220
asnglyasngluhisvalleuglutyrasnproglntyrarglysala
225230235240
serlyslysleutyrgluleuleuserasppheaspthralaphetyr
245250255
aspaspasnasnmetvalleuglythrasplyspheserglngluasn
260265270
thrtyrserargglnprotyrvalserasnglytyrileglyalaarg
275280285
leuproasnileglypheglypheserleuaspthrpheasnprotrp
290295300
thrproaspserlysasnileproaspserleuasnasnglytrppro
305310315320
leuargasnargargphethrglyalatyrvalseraspphetyrser
325330335
ileglnlysserleuasnserthrasnpheprogluleuaspglulys
340345350
glytyrserthrvalileserthrileproglntrpthrasnleugln
355360365
pheserileglupheasnasnargilehistrppheasnproglnmet
370375380
vallysaspgluaspilethrasntyrlysglnasnleusermetlys
385390395400
asnglyvalvalthrthrglumetasptyrleuaspserleuvalhis
405410415
ilelysseraspvaltrpthrhisargargmettyrproleuglyval
420425430
leuasnvalgluilethrleuasnthrglulysleuasntyrasnasn
435440445
asnasnasnasnasnasnasnargasnserasnaspgluglutyrasn
450455460
seraspgluglnvalileleuthrvaltyraspthrleuaspmetglu
465470475480
thrserpheargthrpheleuglnthrserglyileglugluaspasn
485490495
asnalailephemetvalvalhisproaspasnileprotyrserasn
500505510
alaalailetyrserthrcysseralahistyrglyasnserasngly
515520525
trpargthrprolyslysaspphealaphelyslysasnpheserser
530535540
ileglyasnaspaspasnglyglulysileserglntyrthrlysval
545550555560
asnleuserlystyrasnlyslysleuileileasnlystyrserala
565570575
ileileserserglupheasnserasngluglnlysserasnleuglu
580585590
metalalysgluilealaleualaserlysglyasntyrgluserleu
595600605
leulysserhisthrglualatrpalagluleutyrasnaspalaphe
610615620
ilegluileproseraspserleuleuglumetthralaargserser
625630635640
leuphehisileleuserasnthrarglystyrasnvalsergluglu
645650655
argglyleuprovalprovalglyglyleuserseraspsertyrgly
660665670
glymetvalphetrpaspalaaspiletrpmetglnproalavalleu
675680685
prophepheproasnilealalysglnmetserasntyrargasnala
690695700
thrhisglnglnalaleuleuasnalalysgluhisglyhisprogly
705710715720
alaleutyrprotrpthrserglyargphealaasncysserserthr
725730735
glyprocystyrasptyrglutyrhisileasnvalaspvalalamet
740745750
alaserpheseriletyrmetasnglyglygluglyileaspaspasp
755760765
tyrleuargtyrthrthrtrpproleuvalargaspalaalailephe
770775780
phealaserphevalglnpheasnglnthrileglylystyrgluthr
785790795800
tyrasnleuthraspproaspgluphealaasnphevalasnasngly
805810815
alaphethrasnalaglyilelysthrleuleulystrpalathrasp
820825830
ileglyasnhisleuglygluphevalaspproargtrpmetgluile
835840845
serhisasniletyrileproargserthrserasnilethrleuglu
850855860
tyrserglymetasnserservalgluvallysglnalaaspvalile
865870875880
leumetvaltyrproleuglytyrleuasnaspgluserileleuasn
885890895
asnalaileargaspleutyrtyrtyrserglulysglnseralaser
900905910
glyproalametthrtyrprovalphevalalaalaalaalathrleu
915920925
leuasnhisglyserserserglnsertyrleutyrlysservalleu
930935940
protyrleuargalaprophealaglnphesergluglnseraspasp
945950955960
asnpheleuthrasnglyleuthrglnproalaphepropheleuthr
965970975
alaasnglyglypheleuglnserileleupheglyleuthrglyile
980985990
argtyrthrtyrgluvalaspglngluthrlysglniletyrargval
99510001005
leuargpheasnprothrgluleuproargleuproglyglyile
101010151020
alaileargasnphelystyrmetasnglnvalleuaspileile
102510301035
ileaspaspasnasnglythrilevalhislysserglyaspthr
104010451050
proilemetilelysileproaspargserleuilehisasparg
105510601065
aspvalgluprotyrargglyileasnlysthrasnasnleupro
107010751080
valgluaspileasnasnserileglnvalaspasphisvalglu
108510901095
ileleuglnasnglysertyrtyrileleuasnprolysgluglu
110011051110
leulysleuproleuphelysleulysleuasnvallysglyasn
111511201125
ilevalgluasnlysglnilethrasnleuthrgluglyvalpro
113011351140
glyaspvalalavalseralaleuaspglyasnasntyrthrhis
114511501155
trpglnprometasplystyrargproalaargleuleuileasp
116011651170
leuglylysaspasnglnglngluilelysserglymetileleu
117511801185
trpglyglyargproalalysasnpheserileserileleupro
119011951200
hisseraspglnilegluargileleuglulysvalserasparg
120512101215
leugluserthrgluglyserglulysserargthrileglnglu
122012251230
leuleugluaspileglyaspvallysaspthrsermetglugly
123512401245
cysglnasnseraspgluaspargcysthrasnaspleugluser
125012551260
ileleuglntrpthrleuaspaspleuaspasnileleuserasn
126512701275
leuproglnleulysleuleuasnarglyspheilethrileleu
128012851290
lysasphisgluvalileprosergluprophepheprogluile
129513001305
ilegluglnserleuilegluileleuproserasnlysthrtyr
131013151320
phehisileasptyrserlysilelysileglyserasnglylys
132513301335
serleugluaspasnglugluasparglysalaargpheileval
134013451350
leuthrvalasnglyvalpheaspaspaspasnaspserlysgly
135513601365
alathrilelysgluilevalleulysasnaspaspthrgluphe
137013751380
ileaspile
1385
<210>46
<211>1170
<212>prt
<213>鲁氏接合酵母(zygosaccharomycesrouxii)
<400>46
metmetlysileaspleuasnalaaspalaglyaspaspserglnval
151015
proleuargargglucysglngluserprolysalavalarglyslys
202530
valileserasnileilemetservalleualailepheglyleuleu
354045
leuleuleuglytrpglnthralalysglyglnalavalleuproala
505560
alaglnglyproglyileilealalysalathrargserhisaspleu
65707580
glnthrasnaspgluvalarglysalaserlyslysleutyrgluleu
859095
leualaaspthrgluglyalatyrtyraspproaspalametvalleu
100105110
glyserthrilepheserprohisthrtyrserargglnprotyrval
115120125
alaasnglytyrileglyserargileproasnvalglypheglytyr
130135140
alagluaspvaltrpasniletrpalaasnalaaspasplysilepro
145150155160
glyalaleugluasnglytrpproleuargasnargargphealagly
165170175
alaphevalseraspphetyrcysleuglngluserleuasnserthr
180185190
asnpheprogluleuasplysaspglytyrserthrvalilealaser
195200205
ileproglntrpthrglnleuglnpheserleuasnaspglulystrp
210215220
pheaspproglnservalaspsergluaspvalserasntyrthrgln
225230235240
gluleusermetlysasnglyvalvalthrthrcysleuasptrpleu
245250255
aspglyleuleuhisvalgluthrgluvalleualahislyslysile
260265270
tyrthrleuglyilevalsermetlysvalserleuasnvalasparg
275280285
leuprogluaspgluserservalgluleuasnphetrpaspthrleu
290295300
asppheasnthrserhisargservalleualaglylysglytyrasp
305310315320
gluglyleutyrmetvalvalgluprogluasnvalprotyrserasn
325330335
alaserilepheserlyscysgluvalaspglyserlysvalgluphe
340345350
serglupheglyserhisgluglnlysvalthrglnasnalaserval
355360365
ileleuthrargaspasnproglnthrthrilehislystyrvalgly
370375380
ilevalserthrglutyrseraspglyalathrleuserargalalys
385390395400
aspvalalailealaserhisglyasntyrglulysleuserleuala
405410415
hisaspglualatrplyslysiletyraspasnalapheilegluile
420425430
proseraspgluvalleuglumetthralaargserserleutyrhis
435440445
ileleualaasnthrargthrtyrasnvalsergluasnargglyleu
450455460
provalservalserglyleuserseraspsertyrglyglymetval
465470475480
phetrpaspseraspphetrpileglnproglyileleuprophephe
485490495
proaspilealalysasnileasnasntyrargasnalathrhisglu
500505510
glnalaargleuasnalagluglntyrglytyrproglyalaleutyr
515520525
protrpthrserglyargtyralaasncysthrserthrglyprocys
530535540
valasptyrglutyrhisileasnvalaspvalcysleualaserphe
545550555560
seriletyrleuasnglyalagluglyileaspaspglutyrleulys
565570575
tyrthrthrtrpprometvalhisaspalaalagluphephethrala
580585590
phevallystyrasnserthrileaspargtyrgluthrhisasnleu
595600605
thraspproaspgluphealaasnhisileaspasnglyalaphethr
610615620
asnalaglyilelysleuleuleulystrpalathraspileglytyr
625630635640
hisilearggluprovalaspproargtrpmetgluileserglulys
645650655
iletyrileproglnseraspserasnilethrleuglutyrsergly
660665670
metasnserservalgluilelysglnalaaspvalthrleumetval
675680685
tyrproleualapheilethraspgluserileleuasnasnalaile
690695700
lysaspvaltyrtyrtyrsergluhisglnserserserglyproala
705710715720
metthrtyrprovalphevalalaalaalaalaglyileleuasnhis
725730735
glyserserserglnsertyrleutyrlysserleuleuprotyrleu
740745750
argalaprophealaglnphesergluglnseraspaspasnphemet
755760765
thrasnglylysthrglnproalaphepropheleuthralaasngly
770775780
glypheleuglnserileleupheglyleuthrglyileargtyrser
785790795800
tyrgluvalgluproglythrlyslysileglnargileleulysphe
805810815
asnproilegluleuproleuleuproglyglyleualaileargasn
820825830
phelystyrleuaspglnvalleuaspileileileaspaspargasn
835840845
glythrilevalhislysserglyaspglnproileargilelysval
850855860
proaspargthrlysilehisaspargaspilethrprotyrarggly
865870875880
asnglyserthrglymetgluserpheglnasngluaspalathrhis
885890895
glythrseralahistyrtyrthrileasnproglyglugluleuile
900905910
leuproleuphehismetalaproasnvalgluglyasnleuvalglu
915920925
serlysglnvalthrasnleuthralaglyvalproglyaspvalala
930935940
ileserthrleuaspglyasnasntyrthrhistrpglnprovalasp
945950955960
lysserleuproglylysleuleuileaspleuglyalaasnasnser
965970975
glnvalileasnargglymetileleutrpglyglnargproalalys
980985990
asnphethrileserileleuprohisthrgluaspmetglyglnval
99510001005
leuserasnleuthrthrilevalgluserthrasnvalglyasp
101010151020
gluservalleuglualaleugluaspileglyglyleuserpro
102510301035
leuleuserhisserglyaspgluaspvallysasnleuleuser
104010451050
trplysalagluaspvalaspaspilemetleuglyilesergly
105510601065
gluserasnvalileargglylysphevalproileleulysasp
107010751080
tyrlysvalgluproasngluprotyrpheglugluiletyrser
108510901095
lysserileilegluileleuproserasnargthrgluphethr
110011051110
ileasptyrasplysvalglnhisasnserthrthrcyssertyr
111511201125
lysasnleuseraspasncysthrasptrpthrlysthrargphe
113011351140
ilemetvalserilegluglythrtyraspasnaspthraspasp
114511501155
lysglyglythrilelysgluilevalleuleuasn
116011651170
<210>47
<211>1175
<212>prt
<213>副贝氏丝酵母(zygosaccharomycesparabailii)
<400>47
metlysargaspleuthrargasnproserasnvalserthralaala
151015
gluhishisalalysgluprolysaspcysglyilelysthrargleu
202530
leuglyvalsermetvalleuphethrilephethrphepheleuleu
354045
leuglytrppropheglyglyleualaprothrpheseralaalaglu
505560
seralaglyileilearglysthrseraspvalhisthrleuaspphe
65707580
asnlysglualaargargalaserlyslysleutyrgluleuleuval
859095
aspileaspglyalaphetyraspalagluasnmetvalleuglyala
100105110
thrlyspheserprohisthrtyrserargglnprotyrvalalaasn
115120125
glytyrileglyserargileproasnvalglyalaglytyralaglu
130135140
aspthrtrpasniletrpalaseralaglyglugluleuproglyala
145150155160
leuasnasnglytrpproleuargasnhisargphealaglyalaphe
165170175
valseraspphetyrcysleuglulysthrleuasnserthrasnphe
180185190
progluleuaspgluasnglytyrserthrvalileserserilepro
195200205
glntrpthrasnleuglnpheserleuasngluthrvaltrppheasn
210215220
proglnservalthrasnaspasnvalthrasntyrargglngluleu
225230235240
sermetlysasnglyvalvalthrthrhisleuaspcyspheaspglu
245250255
lysvalhisvalgluthrglnilephealahislyslysiletyrpro
260265270
leuglyilevalsermetlysvalalaleuasnileasphisleuile
275280285
proglyglnpheserilegluleuasnphetrpaspileleuaspphe
290295300
asnthrserhisargthrvalleuhisaspleuglytyraspaspgly
305310315320
ilephemetilevalgluproaspasnvalprotyrserasnalaser
325330335
ilepheserthrcysthrvalaspargthrglyvalthrpheasparg
340345350
glygluthrthrtyrgluglnileserglnasnalaservalileleu
355360365
thrlysaspasnproasnvalthrilehislystyrvalglyileval
370375380
serserglutyrtyrasnglyserthrleuasptyralalysgluile
385390395400
alalysserasnargglyasntyrglulysvalserlysvalhisasn
405410415
glualatrpaspalaleutyrasnaspalapheilegluileproser
420425430
aspgluvalleuglumetthrthrargserserleutyrhisleuleu
435440445
alaasnthrargthrtyrasnvalserglualaargglyleuproval
450455460
provalserglyleuserseraspsertyrglyglymetvalphetrp
465470475480
aspalaaspphetrpileglnproservalleuprophepheproasp
485490495
ilealalysasnileasnasntyrargasnalathrhisargglnala
500505510
glnleuasnalaglnglnhisglytyrproglyalailetyrprotrp
515520525
thrserglyargtyralaasncysthrserthrglyprocysmetasp
530535540
tyrglutyrhisileasnvalaspilealaleualaserpheserile
545550555560
tyrleuasnglyalaglnglyileaspaspthrtyrleulystyrthr
565570575
thrtrpproilevalargaspalaalagluphephethrasppheval
580585590
lystyrasnhisthrileglylystyrgluthrhisasnleuthrasp
595600605
proaspgluphealaasnhisileasnasnglyalaphethrasnala
610615620
glyileargleuleuleulystrpalathraspileglyhishisleu
625630635640
arggluprovalaspproargtrpmetasnilesergluasniletyr
645650655
ileproilesergluserasnilethrleuglutyrserglymetasn
660665670
serservalgluilelysglnalaaspvalthrleumetvaltyrpro
675680685
leuglytyrilethraspgluleuvalleuasnasnalailelysasp
690695700
leutyrtyrtyrsergluhisglnserproserglyproalametthr
705710715720
tyrprovalphevalalaseralaalaglymetleuasnhisglyser
725730735
serserglnsertyrleutyrlysserleuleuprotyrleuargala
740745750
prophealaglnphesergluglnseraspaspasnpheleuthrasn
755760765
glylysthrglnproalaphepropheleuthralaasnglyglyphe
770775780
leuglnservalleupheglyleuthrglyileargtyrthrtyrglu
785790795800
valasnproalaserlyslysilegluargvalleulyspheasnpro
805810815
ilegluleuproleuleuproglyglyilealaileargasnphelys
820825830
tyrmetasnglnvalleuaspileileileaspasphisasnglythr
835840845
ilevalhislysserglyasplysserileargilelysvalproasp
850855860
argserleuileargaspargaspvalasnprotyrargglyproasn
865870875880
serserglngluvalvalasnglulysglyhisaspvalglualaleu
885890895
glyasplysserleualaarglysalaglntyrtyrthrleuasnpro
900905910
glyglugluleuileleuproleuphelysmetlyslysasnvalglu
915920925
glyasnilevalgluserlysglnilethrasnleuthrglyglyval
930935940
proglyaspvalalapheseralaleuaspglyasnasntyrthrhis
945950955960
trpglnprovalasplysseralaproalalysleuleuileaspleu
965970975
glyglnasnasnserglnvalileglnlysglymetileleutrpgly
980985990
glnargproalalysasnilethrileserileleuprohisthrglu
99510001005
thrvalglyglnileleuglugluleuthrthrilealagluasn
101010151020
valasnvalglyglugluservalmetglualaleugluaspval
102510301035
glyglythrasppheleuleualaasnhisglyaspglnaspval
104010451050
lysaspleuleusertrpargalaaspaspleuglugluileval
105510601065
argglnleuserasnaspserasnvalmetargglylyspheval
107010751080
proileleugluasntyrprovalvalprothrvalprotyrphe
108510901095
arggluiletyrasplysserileileglnileleuproserasn
110011051110
ilethrgluphethrileasptyrserlysilealahisasnser
111511201125
serargcyssertyrargasnserserasnasncysthrasptrp
113011351140
lyslysthrargpheileleuleuglyileglnglysertyrasp
114511501155
gluaspleuaspprolysglyglythrilelysgluilevalleu
116011651170
leuasn
1175
<210>48
<211>1202
<212>prt
<213>毕赤四盾酵母(tetrapisisporaphaffii)
<400>48
metphepheaspasnvaltyrserglnarglysglulysaspleuval
151015
leuleuiletrppheglnargilepheileilemetleuleuileile
202530
pheleuilepheasncyspheilethrvalglnserilehisileasn
354045
serphepheasnasnlyspheleuarghispheserthrargasnasn
505560
lysleuiletyralaserproilealaglnserasnleuserileleu
65707580
servalglyglugluileserglnvalalathralaarglysthrser
859095
lyslysleutyraspleuleuileasntyrgluaspsertyrtyrasp
100105110
aspgluasnmetvalleuglythraspvalilethrlysasnthrtyr
115120125
thrargglnprotyrvalalaasnglytyrileglyserargilepro
130135140
asnileglypheglytyralaleuaspglutyrseriletrpasnpro
145150155160
aspvalthraspasnprogluleupheasnasnglytrpproleuarg
165170175
asnargargtyralaglyserphevalseraspphetyrserleugln
180185190
lyslysleuasnserthrasnpheprogluileaspaspileglytyr
195200205
serasnvalileserserileproglutrpthrasnilelysleuthr
210215220
valileaspgluasnaspargserpheasnpheasnproglnaspile
225230235240
servalasnaspilethrasntyrhisglnasnleusermetglnasn
245250255
glymetvalthrthraspleuasntrpleuasnasnthrleuhisile
260265270
asnserthrmetphethrhisarglysleutyrproleuglyleuleu
275280285
gluileaspilethrleuasnargglutyrleuprothrasppheala
290295300
thrtyrasnileservalpheaspileleuasppheasnthrserhis
305310315320
argthrpheleuargaspmetglyprosertyraspserserlysmet
325330335
glnglyilephemetvalvalgluprogluasnvalprotyrthrasn
340345350
cysvalilepheserthrthrgluilegluglnleuaspilethrgly
355360365
servalhisargasnilethrleuaspasptyrvalileaspglymet
370375380
asnserserileserglnvalalalysaspilethrleuserthrglu
385390395400
pheprolysleulysiletyrlystyrserserileileserserasn
405410415
tyraspglycyslysasnasnthrtyrglnaspasnleuasngluala
420425430
lyshisleualaleuasnserargglyasnpheglnasnleualaasp
435440445
serasnthrglnalatrpvalaspleutyraspasnalapheileglu
450455460
ileproseraspservalleugluleualaalaargserserleuphe
465470475480
hisleumetserasnalaargleuhisasnilesergluasparggly
485490495
leuproleuprovalserglyleuserseraspsertyrglyglyleu
500505510
valphetrpaspalaaspvaltrpmetglnproalaileleuprophe
515520525
pheproaspvalalalysasnileasnasntyrargasnalathrhis
530535540
lysglnalaglnleuasnalalysglntyrglytyrasnglyalatrp
545550555560
tyrprotrpthrserglyglypheserasncysthrserthrglypro
565570575
cysileasptyrglutyrhisileasnvalaspilealaleualathr
580585590
pheseriletyrmetasnglyalaaspglyileaspaspthrtyrleu
595600605
lystyrthrvaltrpproilevalargaspalaalailealaphethr
610615620
glutyrvalglntyrasplysglnleuaspvaltyrgluthrpheasn
625630635640
leuthraspproaspgluphealaasnhisvalasnasnglyalaphe
645650655
thrasnalaglyilelysileleumetlystrpalavalaspilegly
660665670
asnhisleugluglulysvalaspproiletrplysalaileasnglu
675680685
lysilevalileprolysthrserserasnilethrleuglutyrser
690695700
glymetasnserthralagluilelysglnalaaspvalproleuleu
705710715720
valtyrproleuglytyrilethraspgluserileleuasnasnala
725730735
ileargaspleutyrtyrtyrsergluhisglnalahisthrglypro
740745750
alametthrtyrprovalphevalalaalaalaalaglyleuleuasn
755760765
hisglyserserserglnsertyrleutyrlysserileleuprotyr
770775780
leuargglyprophealaglnphesergluglnseraspaspasnphe
785790795800
leugluasnglyglyleuhisproalatyrpropheleuthralaasn
805810815
glyglypheleuglnserileleupheglyleuthrglyileargtyr
820825830
sertyrlysvalaspseraspthrasnlysilealaargleuleulys
835840845
pheasnproilelysleulysleuleuproglyglyleulysilearg
850855860
asnphelystyrmetglyglnvalleuaspileileileaspasptyr
865870875880
asnglythrileilehislysserglyasnlysthrilethrilegln
885890895
ileproaspargserserileargaspglnaspileasnprotyrarg
900905910
glylysasnlysthrlysgluaspasnargileleuserlysarglys
915920925
serasniletyrgluleulysproasngluasnphethrileproleu
930935940
phevalthrgluleuasnalaproglyasnilevalgluserlysgln
945950955960
ilethrasnleuthrgluglyvalalaglyaspvalalametserala
965970975
valaspglyasnasntyrthrhistrpglnprovalasnlysalaglu
980985990
thralaargleuleuileaspleuglyproglyasngluglnthrile
99510001005
thrglyglymetpheleutrpglyglnargproalalysasnval
101010151020
serileservalleuprohisserglnalathrgluglnleuphe
102510301035
glnasnilethrtyrphealagluhispheglupheproasnarg
104010451050
gluargthrileleuglnleuileglngluvalaspasnilethr
105510601065
gluprogluvalcysglnilepheserseraspglyvalcysasp
107010751080
alaglyphethrglnleuleuasntrplysserlysaspileasp
108510901095
gluleuileglnglnpheproasnthrproglyilearggluasn
110011051110
pheilethrilevalaspasnleuservalleuproserglupro
111511201125
tyrtyrproglutyrtyrgluglnalaleuilegluileleupro
113011351140
serasnlysthrlyspheservalasptyrserlysvalglutyr
114511501155
tyrlyslyssergluvalphegluglnserglntrpprolysthr
116011651170
argpheileilevalserileglnaspthrtyrasplysaspleu
117511801185
aspprolysglyserthrilelysgluileileleutyrser
119011951200
<210>49
<211>1180
<212>prt
<213>棉假囊酵母(eremotheciumgossypii)
<400>49
metalaaspthralaserleuproproglnargaspseralaleugly
151015
methisglyprohisglyglyleutyrmetprovalalaglnglypro
202530
leuglnalahisalaserproargleuvalservalargmetvalleu
354045
serserilethralaleualaleuvalalavalvalthrvalleugly
505560
thralaglnproalaargprothralaproleualaalaalaaspglu
65707580
glnphetrpvalalaglnhisargseralaserlysglnleutyrgln
859095
leuvalhisglysergluleuserphetyraspgluglyargaspval
100105110
leuglythrthrgluleuserargasnmettyrserargglnprotyr
115120125
valalaasnglytyrileglyserargvalproasnvalglyphegly
130135140
tyralaalaaspglugluasniletrpthraspalaservalprogly
145150155160
alaleuasnasnglytrpproleuargasnproargtyralaglyser
165170175
phevalseraspphetyrserleuglnalaargleuasnserthrasn
180185190
pheprogluleuaspglugluglytyrserthrvalilealaserile
195200205
proglutrpthraspleuargvalargalaaspglyalagluleugly
210215220
alagluthrvalalaleugluaspmetglyglytyrvalglnasnmet
225230235240
serleualaaspglyvalvalthrthrglutyrvaltrpargglyleu
245250255
servalargalathrvalalaalahisargserglutyrproleugly
260265270
leuvalglnleugluvalalaleucysglyaspthrgluproargglu
275280285
valgluvalargaspvalleuasnphethrthrserhisargthrval
290295300
leuargglualaglyhisaspgluaspglyiletyrmetargvalglu
305310315320
progluasnvalprotyrserglualaalaleutyrservalpheglu
325330335
valargglyglygluglyservalglnprogluargalaalaalagly
340345350
alathrvalalaglntrpvalargvalargleuthralaalaglnpro
355360365
argvalvalvalarglystyrvalglyvalvalserserglutyrasn
370375380
thralaglyglyserasnleuglualaalaargalaalaalaleuala
385390395400
histyrglyalapheaspglyalaleuvalserhisargalaalatrp
405410415
seralaleutyrglyasnalaserilegluileproserasppheleu
420425430
leugluleualaalalyssersermetphehismetleualaasnthr
435440445
argalahisasnvalseralathrargglyleuprovalprovalthr
450455460
glyleuserseraspsertyrglyglymetvalphetrpaspserasp
465470475480
valtrpmetleuproglyleuleuprophepheproaspilealaarg
485490495
gluileserasntyrargasnalathrhisalaglnalavalalaasn
500505510
alaarghistyrasntyrserglyalaleutyrprotrpthrsergly
515520525
argtyralaasncysthrserthrglyprocysvalasptyrglutyr
530535540
hisileasnileaspilealametserserleuseriletyrmetasn
545550555560
glyalaaspglyileglygluasptyrleuargtyrthrthrtrppro
565570575
leuleuargaspalaalaleuphephethrglutyrvalargtyrasn
580585590
gluthrleuaspalatyrthrthrhisasnleuthraspproaspglu
595600605
phealaasnpheileaspasnglyalaphethrasnalaglyilelys
610615620
ileleuleuargtrpalaileaspvalglythrhisleugluglupro
625630635640
valaspthrlystrpglngluileserasplysilehisileprothr
645650655
sergluthrasnilethrleuglutyrthrglymetasnalathrval
660665670
aspilelysglnalaaspvalleuleumetvaltyrproleuglytyr
675680685
ilethraspgluserileleuasnasnalaileglnasnleutyrtyr
690695700
tyrsergluargglnseralaserglyproalametthrtyrproval
705710715720
phealaalaalaalaalathrleuleuasnhisglysersersergln
725730735
sertyrleutyrlysalavalvalprotyrleuargalaprophephe
740745750
glnphesergluglnseraspaspasnpheleuthrasnglyleuthr
755760765
glnproalaphepropheleuthralaasnglyglytyrleuglnser
770775780
leuleupheglyleuthrglyleuargtyrsertyrthrvalasnpro
785790795800
gluthrlyslysmetgluargleuleulyspheserprovalargmet
805810815
proleuleuproglyglyileargileasnasnphelystyrleugly
820825830
glnvalleuaspileserileaspasphisasnalathrilealahis
835840845
lysglnglyasnthrproilehisilelysvalproaspargserile
850855860
leuargaspargaspvalprovaltyrlysglyseralaleuglnala
865870875880
argaspvalileprotyrhisgluleuserasnserasntyrphethr
885890895
valasnproglygluthrleuthrleuprovaltyrgluprogluleu
900905910
asnileglnglyasnilevalgluglyargglnilethrasnleuthr
915920925
glnglyvalproglyaspvalproileserileleuaspglyasnasn
930935940
tyrthrhistrpglnpropheasplyssergluargalaleuleuleu
945950955960
ileaspleuglyserglugluglutyrgluilethrthrglylysile
965970975
leutrpglyalaargproalalysasnpheserileserileleupro
980985990
asnserlyshisilethrgluileleuthrlysleuthralametmet
99510001005
aspglyargasnthraspleuvalsercysserlyscyshisala
101010151020
valserserserglnhisleuleuglyglyleualaasnvalthr
102510301035
aspserlysglyleualaalaileaspglygluthrvalaspmet
104010451050
glyilearggluilepheargtrpasnleupheaspleuprothr
105510601065
ileserserileileproglualaalaasnilesergluserphe
107010751080
valthrvalleugluasntyrglnvalthrprosergluprotyr
108510901095
tyrglugluvalvalarglysserglnilevalileleuproser
110011051110
asngluthraspphecysileasptyralaalavalprolysleu
111511201125
asnprothrtyrthralavalasnleuseralaaspaspthrasn
113011351140
trparglysthrargphevalilevalalavalgluglysertyr
114511501155
aspaspaspaspaspglnlysglyglythrilelysgluileala
116011651170
leumetvalalaprolysasn
11751180
<210>50
<211>1215
<212>prt
<213>鼻窦假囊酵母(eremotheciumsinecauda)
<400>50
metargaspvalilethrserleupheleulysproglnglnargarg
151015
thrglyserthrcysvalaspilegluseraspprovalserasnlys
202530
alathraspphethrvalglyglngluprovalilesergluproarg
354045
lysserthrlysthrleuasnleumetserargmetmetalaalaleu
505560
thrvalvalileleuvalvalthrleuleualapropheservalgly
65707580
thrglyargaspmetileleuleuserprovalprogluserarglys
859095
valglyglyglylysgluglymetaspalagluleuglnaspargphe
100105110
argvalserglnhisargserthrserlyslysleutyrgluleuleu
115120125
argaspserglulysthrphetyraspgluglugluglnvalleugly
130135140
thrthrvalalaserargasnthrtyrserargglnprotyrvalala
145150155160
asnglytyrileglyserargileproasnvalglypheglytyrala
165170175
leuaspgluleuserleutrpthrglyaspseraspileproglyala
180185190
leuaspasnglytrpproleuargasnglnargphealaglyalaphe
195200205
valseraspphetyrglyleuvalgluasnileasnserthrasnphe
210215220
arggluleuaspglugluglytyrthrthrvalileseralailepro
225230235240
glntrpthraspleuargleuservalphelysaspasnaspaspasn
245250255
protyralathrleuserprovalgluleuaspleuserglnvalser
260265270
glntyrthrglnasnleuserleuglnaspglyilevalcysthrglu
275280285
pheasntrpmetasnleuleuhisilelysthrservalilealahis
290295300
arglysilephethrleuglyleuvalglumetgluvalserleuleu
305310315320
gluaspvallysleuglylysglyglyserleuhisvalgluileglu
325330335
aspmetpheaspphegluserserargargthrpheleuargasphis
340345350
glytyraspaspgluglyiletyrmetvalvalgluprogluasnval
355360365
protyrserasnalaserleutyrserargcysaspvalgluglygly
370375380
sergluvalseraspvalgluargtyrvalthrasnasnthrvalile
385390395400
glnserhisservaltyrleuasphisserserproilevalvalval
405410415
glnlystyrvalglyilevalserthrglutyrasnglugluproile
420425430
serasnleualaargalalysgluvalvalglnaspcyspheglyser
435440445
pheaspalaleuleusertrphisargglualatrpalaasnleutyr
450455460
alaaspalaserilegluileproserasppheleuleugluleuala
465470475480
alalysserserleutyrhisleuleualaasnthrargserglnasn
485490495
valserserserargglyleuprovalalavalalaglyleuserser
500505510
aspsertyrglyglymetvalphetrpaspalaaspthrtrpileleu
515520525
proglyileleuprophepheproaspilealalysglumetalaasn
530535540
tyrargasnalathrhispheglnalailegluasnalaglnlystyr
545550555560
asntyrserglyalaleutyrprotrpthrserglyargphealaasn
565570575
cysthrserthrglyprocysvalasptyrglutyrhisileasnile
580585590
aspilealametserserleuseriletyrmetasnglyalaaspgly
595600605
valaspaspasptyrleuargtyrthrthrtrpproleuileargglu
610615620
alaalaasnphephethrglutyrvalargtyrasngluthrleuasp
625630635640
alatyrthrthrhisasnmetthraspproaspgluphealaasnphe
645650655
ileaspasnglyalaphethrasnalaglyilelysthrleutyrlys
660665670
trpalathraspvalglyasnhisleugluglugluileasppromet
675680685
trpsergluileserglulysilehisileprothrserprothrasn
690695700
ilethrleuglutyrthrglymetasnalathrvalaspilelysgln
705710715720
alaaspvalmetleumetvaltyrproleuglytyrilethraspglu
725730735
serileleuasnasnalaileglnasnleutyrtyrtyrsergluarg
740745750
glnseralaserglyproalametthrpheprovalphevalalaala
755760765
alaalaalaleuleuasnhisglycysserserglnsertyrleutyr
770775780
lysservalvalprotyrleuargserpropheleuglnpheserglu
785790795800
glnseraspaspasnpheleuthrasnglyleuthrglnproalaphe
805810815
propheleuthralaasnglyglytyrleuglnalaleuleuphegly
820825830
leuthrglyleuargtyrsertyrthrvalasnprogluthrlyslys
835840845
metgluargleuleulyspheasnproilealaleuprometleupro
850855860
glyglyileargileasnasnphelystyrmetglyglnvalleuasp
865870875880
ileservalglyasptyrasnalaseriletyrhislystyrglyasp
885890895
valproileasnilelysvalproaspargservalleuargasparg
900905910
gluilepropheproserserserasnlysvalglnalaargaspmet
915920925
ileasptyrthrgluleulysaspserasntyrhisthrvaltyrpro
930935940
glyglulysleuvalleuprovalphethrproglyleuasnileglu
945950955960
lysasnilealagluglylysglnilethrasnleuthrargglyval
965970975
proglyaspvalalailesermetvalaspglyasnasntyrthrhis
980985990
trpglnpropheasnlyssergluargalaleumetleuileaspleu
99510001005
glyserglugluaspphegluilethrglyglylysilevaltrp
101010151020
glyalaargproalavalasnleuserleuserileleuprohis
102510301035
tyrgluasnmetthralavalleuaspservalilegluthrtyr
104010451050
glnlysasnalaserserlysleuthrservalarghislysgly
105510601065
serserglyglyvalproasnglyservalhisthrserproasp
107010751080
ileileglyserthrileaspmetglyilearggluilepheasn
108510901095
trpasnleuileasppheaspgluileseralailealaproglu
110011051110
valleuprolyspheargasnphevalthrileleuaspasphis
111511201125
provalgluprothrglnprophephehisaspvalpheasngln
113011351140
serglnilevalileleuproserasngluthraspphetyrile
114511501155
asptyrasplysvalalagluleuasnproglytyrthralaile
116011651170
aspphelyslysasnaspasnthrtrparglysthrargpheval
117511801185
metvalthrvalgluglyvaltyraspgluaspaspaspprotyr
119011951200
glyalathrilelysgluilealaleumetserthr
120512101215
<210>51
<211>1133
<212>prt
<213>米拉氏拉茜斯酵母(lachanceamirantina)
<400>51
metlysthrglulysalalysserargleuphetyrservalleuthr
151015
cysleuleuvalleuglytrpphethrvalthrsertyrleuglnlys
202530
leulysproglyleuservalphegluserthrargargpheserglu
354045
argvalargasnargarglysserserglnglnleutyrgluglnleu
505560
seraspseraspaspthrtyrtyraspgluvalhistrpcysvalgly
65707580
thrsertyrphesergluasnthrpheserargglnprotyrvalser
859095
asnglytyrileglyserargileproasnvalglyleuglytyrala
100105110
leuaspthrleuasniletrpthralaasnserservalproglyala
115120125
leuaspasnglytrpproleuargasnglnargphealaglyalaphe
130135140
valseraspphetyrcysleugluprolysleuasnserthrasnphe
145150155160
progluleuaspaspaspglytyrserthrvalleuseralailepro
165170175
glntrpthraspleuglnpheservalargasnglyserleulysphe
180185190
seralaglnaspvalargleugluaspvalthrasntyrhisglnasn
195200205
leusermetglnthrglyvalvalserthrgluleuasptrpleugly
210215220
pheleuhisvallysserservalleualahisargserlyshisser
225230235240
leuglyilevalthrleugluleuasnvalasnproglytrpaspval
245250255
aspgluvalaspleugluleucysaspcysleuasntyrthrthrala
260265270
argargvalalaleuglnaspileglyhisaspserglyglyiletyr
275280285
metthrvalasnproaspasnvalprotyrserseralaseriletyr
290295300
serserphegluleuleutyraspglygluargserglylysserglu
305310315320
pheargpheaspaspasnargthrthrileserglncysvalserval
325330335
lysleuglualaaspglyproileleulysalaasnlysphethrgly
340345350
ilemetserthrglutyrglyaspglyargargaspglnthrasnleu
355360365
gluphealaargservalvallysgluserleuglysercyshislys
370375380
leuthrgluserasnargalaalatrpglualaleutyrseraspala
385390395400
hisilegluileproseraspserleuleugluleualaalaargser
405410415
serleutyrhisleuleualaasnthrargargpheasnvalserala
420425430
gluargglyleuprometprovalserglyleuserseraspsertyr
435440445
glyglymetvalphetrpaspalaaspiletrpmetmetproserleu
450455460
leuprophepheprogluilealaarghisilevalasntyrargasn
465470475480
glythrhisglnglnalavalleuasnalaglnlystyrasptyrser
485490495
glyalaleutyrprotrpthrserglyargphealaasncysthrser
500505510
thrglyprocysvalasptyrglutyrhisvalasnpheaspileala
515520525
metalaserpheleumettyrleuasnglygluglygluasptyrleu
530535540
argtyrthrthrtrpproleuvalargaspalaalalysphepheala
545550555560
glutyrvalglnileasnhisthrthrglyglntyrgluthrpheasn
565570575
leuthraspproaspglutyralaasnphevalasnasnglyalaphe
580585590
thrasnalaglyilelysthrleumetlystrpalathraspilegly
595600605
tyrhisleuglyasngluvalaspprolystrpasnhisileserlys
610615620
asniletyrileprometalagluserasnilethrleuglutyrser
625630635640
glymetasnalaservalgluilelysglnalaaspvalvalleumet
645650655
thrtyrproleuglytyrilethraspgluthrileleulysasnala
660665670
ileargasnleutyrtyrtyrsergluargglnseralaserglypro
675680685
alametthrtyrprovalphevalalaalaserserleuleuasnhis
690695700
glycysserserglnsertyrleutyrlysservalleuprotyrleu
705710715720
argalaprophemetglnphesergluglnseraspaspasppheleu
725730735
thrasnglyleuthrglnproserphepropheleuthrglyasnala
740745750
glypheleuglnglyileleupheglyleuthrglyleuargtyrser
755760765
tyrgluvalaspproalaserlysthrileasnargpheleulysphe
770775780
ileprometlysleuproleuleuproglyglyilealaileargasn
785790795800
phelystyrmetglyglnvalleuaspvalileileaspasplysasn
805810815
alathrleuilehislysglnglyaspleualavalthrleulysthr
820825830
pheasnargserpheasnthraspargglnleuglyalahishismet
835840845
trpthrgluleuleualalysasngluserleuproalathrphetyr
850855860
thrleuasnproarggluthrleuserleuproleuphegluproleu
865870875880
leuasnileaspasnasnleualagluglylysglnilethrasnphe
885890895
thralaglyvalproglyaspvalvaltyrseralavalaspglyasn
900905910
asntyrthrhistrpglnproleuasnlysaspmetargalalysleu
915920925
leuileaspleuglyproaspasnasplysproleuthrargglymet
930935940
ileleutrpglylysargproalaargasnvalservalservalleu
945950955960
proserhisglylystyrvallysglyilelysilelyslysargglu
965970975
gluaspgluthrglyphecysproleuthrileleuargglyargcys
980985990
asnvalalaseralailealaleuprogluaspserhisilepheasp
99510001005
thrgluleuargaspmetleuasnsergluargleuaspleuglu
101010151020
glnleuthrargleuserproasntyrservalphethrasparg
102510301035
phevalthrileleugluasppheglnvalpheproserglupro
104010451050
tyrtyrgluthrleuglnargasnlysserilevalileleupro
105510601065
serasnlysthrgluphevalpheasptyrglugluleucysmet
107010751080
argalacysasnaspthrhisvalcysalagluasnalailethr
108510901095
glyleuproproproargphevalleuleuservalgluglythr
110011051110
tyraspaspasplysaspprolysglyalathrileglugluile
111511201125
alapheleuserasp
1130
<210>52
<211>1070
<212>prt
<213>近平滑假丝酵母(candidaorthopsilosis)
<400>52
metaspilegluasnasnglyiletyrilegluaspserserasnthr
151015
gluglyilelyslysserleuargaspasnlysvaltyrilevalser
202530
metthrleuvalalaasnileileleuphetyrserleuhisleuleu
354045
asnalaargphecysglualapheprophevalleuproargserglu
505560
lystyrthrleuglnglnseraspserasnileglyaspphevalasn
65707580
serphealaasnlysgluilepheserglnileglnphesergluhis
859095
alaphetyrasparggluleuasnvalvalglythrileglupheser
100105110
lystyrasnglntyrglnlysglnprotyrvalalaasnglytyrile
115120125
glyserargileproasnleuglyglnglyphethrtyraspglnleu
130135140
thrasnserproaspserlyslysaspaspleutyrasnglytrppro
145150155160
leupheasplysargpheserglyalapheilealaglyphetyrasp
165170175
leuglngluasnthrthrglyasnasnpheprogluleuleulysasn
180185190
glytyrgluservalileseralavalproglntrpthrthrleulys
195200205
leuvalilehisargaspserlysasntyrthrleuaspproserser
210215220
proaspglyileglygluilethrasntyrvalglnasnmetserleu
225230235240
serthrglyilevalthrthrglnphethrtrpleuaspleuleuasp
245250255
vallystyrthrvalleualahisargsergluileasnleuglyleu
260265270
vallysvalaspileglnasnlysglyasnaspthrilegluleuasp
275280285
valileaspgluleuasnpheaspthralaglnargcysgluleuasn
290295300
serileglyhisaspasnserglyiletyrvalserpheserproleu
305310315320
asnleuasptyrileserglyalailetyrserthrleuhisleuasp
325330335
aspilethrargargserasnasnlysglnthrlysglnthrserser
340345350
ileargleuasnalaglnaspserthrglnpheglylysthrvalgly
355360365
ilealaserthraspleuasnproserlysphegluasnthrargasp
370375380
thrleuglnphealalyslysvalserglnlyspheglutyrleuasp
385390395400
leuileilelysserhisvalglnalatrplysgluvalthrglylys
405410415
alaproserleuserpheproasnaspproleuleuaspleuglyser
420425430
lysalaservaltyrhisleuleualaasnthrargalaaspalagln
435440445
glyleuthrglyalaleuglyvalalaglyleuserseraspsertyr
450455460
glyglymetvalphetrpaspthraspphetrpmetphelysalaile
465470475480
leualaleuasnproserhisalalysserilevalasntyrargval
485490495
histhrhisgluglnalaleulysasnthrproserglyhisglugly
500505510
alaalatyrprotrpthrserglyargpheglyasncysthralathr
515520525
glyprocysleuasntyrglutyrhisileasnseralailealamet
530535540
alaalatrpgluleutyrleuserglyalavalaspaspglntyrleu
545550555560
gluserthrvaltyrproleuileasnaspalaalalysphetyrala
565570575
asptyrvalthrsertyrasnaspthrileasplysphevalthrasn
580585590
asnleuthraspproaspglutyralaasnhisvalaspasnglyala
595600605
tyrthrasnalaglyilealaleuleumetlystrpileasnaspile
610615620
glyaspvalleuasnvallysmetprogluiletyrthrgluileser
625630635640
glnlysmettyrleuprothralaasnasnserglnasnilethrleu
645650655
glutyrserglymetasnserservalglyilelysglnalaaspval
660665670
valleuleuthrtyrproleuglnasnserleuvalaspaspglugln
675680685
alatyrthrasnmetgluphetyrserglylysglnvalsertyrgly
690695700
proalametthrpheserilepheserilevalalaalaasnleuala
705710715720
serserglycysalaserglnsertyrleuhislysalametglnpro
725730735
tyrleuargglyprophealaglnphesergluglnasnasnaspasp
740745750
tyrlysserasnglyglythrhisproalaphepropheleuthrala
755760765
hisglyglyphevalglnalavalilehisglyleualaglyleuarg
770775780
hissertyralaileaspaspglylysileserargserleupheleu
785790795800
aspproilealaleuprocysleuglyasnglyvalglntyrsergly
805810815
ilehistyraspasnhisthrilesermetasnilethrgluserthr
820825830
phethrilelysasngluglylysthrasnasnargalaasnasptyr
835840845
ileserilethrilealaaspargasnproglyargglythrtyrlys
850855860
leuasnaspasngluglulysserphelysleutyrglnproasnlys
865870875880
asnphegluaspserileserglucysglyleualathrphetyrasn
885890895
ilethrgluseralaproglyaspserserpheserileasnaspgly
900905910
aspasnthrthrargtrpglnalalystyrasnaspthrthrglylys
915920925
valleuileaspleuhisserilelysasnilethrglyvalilephe
930935940
asntrpalaasplysproprolyslysvalthrleuserlystyrser
945950955960
aspthrlysphelysalavalthrasppheleualalysvalaspphe
965970975
glyasnprovaltyrlysasntyrargphealaasnproaspglulys
980985990
leuleuasnglnseraspalapheglugluvalhissergluaspval
99510001005
leuileseralapropheserasnlysglupheglulysvalleu
101010151020
valprovalarghisasnthrthrsertrpaspileasnleulys
102510301035
thrargpheleuleuleugluvalaspserilehisasnthrval
104010451050
proilegluaspasptyrglyglyalalysilealagluvalser
105510601065
phephe
1070
<210>53
<211>1044
<212>prt
<213>麦芽糖假丝酵母(candidamaltosa)
<400>53
metilephephealaasnleuphevalleupheserleuhisleuleu
151015
tyralalysphecysgluglytyrproasnvalvalprovallysile
202530
argserthrglyargilehisargproasnleuglulysleuvalasn
354045
serprogluasnlysglnilepheserglnleulyspheserasphis
505560
alaphetyraspthrthralaasnthrvalglythrilegluphepro
65707580
valpheasnglntyrglnargglnprotyrvalalaasnglytyrile
859095
glyserargileproasnleuglyhisglyphethrtyraspglnleu
100105110
thrasnserseraspalaasnaspaspaspleuvalasnglytrppro
115120125
leupheasnlysargtyralaglyalapheilealaglyphepheasp
130135140
ileglnlysasnthrthrglythrasnpheprogluleuleugluasn
145150155160
glytyrgluservalilealaalavalproglntrpthrserleuser
165170175
leuservalthrileglyglylysasntyrthrleuaspproalaleu
180185190
gluprogluleuileglyasnilethrasntyrvalglnasnleuser
195200205
metseraspglyilevalthrthrglnphethrtrpleuasnserile
210215220
aspvallystyrgluvalleualahisargthrgluileasnleugly
225230235240
leuvalasnleuglnileserasnargglyglnglnserileaspval
245250255
ilevalthraspgluleuaspphegluthrserlysargcysglnleu
260265270
asnlysalaalapheaspaspglnglyiletyrvalthrpheserpro
275280285
hisaspleuasptyrvalhisglyalailetyrserthrleuileser
290295300
seraspalaserprocyslysserserasngluthrvalthrsergln
305310315320
serleuglnleuserleuglucysasnalaasnphegluvalthrlys
325330335
valvalglyvalvalserseraspleuaspproasnserphelysser
340345350
seraspaspvalleuglntyralalyslysileserglnlystyrasn
355360365
asnvalaspglnileileasnserhislysgluglytrpsergluile
370375380
leuglnhisserproleuilethrpheproseraspproleuleuasn
385390395400
leuglyalaargalaserilephehisleuleualaasnthrargpro
405410415
aspalaglnglyvalthrglyalaleuglyvalglyglyleuserser
420425430
aspsertyralaglymetvalphetrpaspalaaspleutrpmetleu
435440445
asnglyileleuprophealaproalahisalalysserleuvalasn
450455460
tyrargleuhisthrhisasnglnalailegluasnvalproglugly
465470475480
tyrasnglyalavaltyrprotrpthrserglyargpheglyasncys
485490495
thralathrglyprocysleuasptyrglutyrhisileasnmetala
500505510
valsermetalaalatrpglnleutyrileserglyalaalaaspasp
515520525
asppheleualaaspvalalatyrproleuileasnaspalaalathr
530535540
phepheserglutyrvalthrhispheasnasnthrvalasplystyr
545550555560
valthrlysasnleuthraspproaspglutyralaasnhisvalasp
565570575
asnglyalatyrthrasnalaglyilealaleuvalmetlystrpala
580585590
glnilevalglyasnhisleuglylysaspvalproasnthrtyrlys
595600605
aspilethrglulysmetpheleuprothrglyaspasnserglnasn
610615620
ilethrleuglutyrserglymetasnserservalglyilelysgln
625630635640
alaaspvalilemetilethrtyrproleugluasngluleuileasp
645650655
gluaspglnalatyrthrasnmetgluphetyrsermetlysglnval
660665670
sertyrglyproalametthrpheproilepheserilevalalaala
675680685
asnleualaprothrglycysalaserglnsertyrleuglnlysala
690695700
ileglnpropheleuargglyprophealaglnphesergluglnasn
705710715720
asnaspasnpheleuthrasnglyglythrhisproalapheprophe
725730735
leuthralahisglyglypheleuglnalathrleuglnglyleuthr
740745750
glymetargtyrglyphethrvalaspgluglnalaserlysleuval
755760765
argvalleuasnleuaspproilealaleuprocysleuglyaspgly
770775780
valglnpheaspglyilelystyraspasnhisthrilesermetser
785790795800
ileasnglnthrhisphethrilelysasnthrglylysthraspser
805810815
asnglyglyasnserpheilethrileserleualagluargasnpro
820825830
asphisglyglntyrthrileasnaspasngluglulysserpheala
835840845
leuphelysproaspthrserpheileaspserileserglucysgly
850855860
glnalaaspphetyrasnilethrgluseralapheglyaspserpro
865870875880
ileserileasnaspglyaspasnthrthrargtrpglnvallystyr
885890895
asnaspthrvalglylysileleuvalaspphelysserphelysasn
900905910
ileserglyvalthrpheasntrpalaasplysproprolysasnleu
915920925
lysleuserlystyrserglyaspglnphethrthrvalthraspphe
930935940
phealalysvalasppheglyasngluleutyraspsertyrlystyr
945950955960
alaasnprogluasplysiletyrasnglnsergluvalpheglnglu
965970975
ilehisseraspservalgluileseralapropheaspasnaspglu
980985990
pheserglnvalleuvalprothrarghisasnthrthrileleuasp
99510001005
leugluleuserglyargpheleuleuilegluvalaspalaile
101010151020
hisasnthrvalproileaspglyasppheglyglyalalysleu
102510301035
alagluvalvalphetyr
1040
<210>54
<211>1083
<212>prt
<213>热带假丝酵母(candidatropicalis)
<400>54
metglyarglysproserserileileprogluglugluvalaspglu
151015
aspglugluaspserlysleuleupheleuleuasnpheleulysser
202530
leuproilelyslysiletrpilevalmetilevalphealaasnleu
354045
phevalpheileserpheglnleuphetyralalysphealahisgly
505560
pheproglyvalileproleulysalaargserprogluglyleuhis
65707580
thrvalaspleuserlysleuileaspserglngluasnlyslysile
859095
pheserglnmetlyspheseraspglnalaphetyraspthrthrasp
100105110
asnvalvalglythrthrgluphethrglupheasnglntyrglnarg
115120125
glnprotyrvalalaasnglytyrileglyserargileproasnile
130135140
glyglnglyphealatyraspglnleuserasnserserglualaasn
145150155160
gluaspaspleuserasnglytrpproleupheasnlysargtyrala
165170175
glyalapheilealaglyphetyraspileglnlysasnthrthrgly
180185190
thrasnpheprogluleuleugluasnglytyrgluservalileala
195200205
alavalproglntrpthrserleuserleuservalgluileaspgly
210215220
glnvaltyrileleuaspproalaleuaspserglnaspmetglygly
225230235240
ileserasntyrvalglnasnleuserleuseraspglyilevalser
245250255
thrglnphethrtrpleuasnlysleuasnvallystyrgluvalleu
260265270
alahisargthrasnileasnleuglyleuvalaspleuglnileser
275280285
asnileglyasngluasnleugluleuthrvalthraspserleuasp
290295300
pheasnthrserglnargcysglnleuasnaspvalserthraspasp
305310315320
glnglyileserilethrpheserprohisaspleuasptyrilehis
325330335
glyalailetyrserlysleuvalleuaspglygluilethrargglu
340345350
valglnthrtyrglulysvalthrglnserserlysilethrilegly
355360365
cysasnserasnilelysileserlysphevalglyvalalaserser
370375380
aspleuglnproaspglnleulysthrseraspaspvalleuglnleu
385390395400
alalysservalalaglnglucyshisasnalagluglnileileasp
405410415
serhislysglnalatrpsergluvalileasnasnglyproleuval
420425430
thrpheproseraspproleuleuasnleuglyalaargalaserval
435440445
phehisleuleualaasnthrargproaspalaglnglyvalthrgly
450455460
alaleuglyvalglyglyleuserseraspsertyralaglymetval
465470475480
phetrpaspalaaspleutrpmetleuasnglyileleupropheala
485490495
proserhisalalysserleuileasntyrargleuhisthrarggln
500505510
glnalailegluasnvalprogluglytyrglnglyalavaltyrpro
515520525
trpthrserglyargpheglyasncysthralathrglyprocysleu
530535540
asptyrglutyrhisileasnmetalavalalametalaalatrpgln
545550555560
leutyrileserglyalaalaaspaspleutyrleugluthrileala
565570575
tyrproileiletyraspalaalaasnphepheserasptyrvalval
580585590
asnpheasnaspserleuasnlystyrthrthrserasnleuthrasp
595600605
proaspglutyralaasnhisvalaspasnglyalatyrthrasnala
610615620
glyilealaleuvalmetargtrpalaglnvalileglyglyhisleu
625630635640
glylysvalvalproglnleupheglnaspilegluglnasnilephe
645650655
leuprothralaasnasnserglnasnilethrleuglutyrsergly
660665670
metasnserservalglyilelysglnalaaspvalilemetmetthr
675680685
tyrproleugluasngluleuileaspaspgluglnalatyrileasn
690695700
metgluphetyrsermetlysglnvalsertyrglyproalametthr
705710715720
pheproilepheservalvalalaalaasnleualaprothrglycys
725730735
alaserglnsertyrleuglnlysalaileglnpropheleuarggly
740745750
prophealaglnphesergluglnasnasnaspasntyrleuthrasn
755760765
glyglythrhisproalaphepropheleuthralahisglyglyphe
770775780
leuglnalathrleuglnglyleuthrglyleuargtyrasptyrasp
785790795800
ileaspgluasnglylysleuvalargileleuargleuaspproile
805810815
glyleuprocysleuglugluglyvalserpheasnglyileglntyr
820825830
aspasnhisthrilesermetserileasnglnthrhisphethrile
835840845
thrasnlyscyslysthrasnproasnalaasnasphisilethrile
850855860
leuleualagluargasnproaspasnglyglntyrvalileasnasp
865870875880
glygluthrlysserphealaleuphelysproaspileserphepro
885890895
aspserileserglucysglyglnalaserphetyrasnvalthrglu
900905910
glyalapheglyaspalaserileserileasnaspglyaspasnthr
915920925
thrargtrpglnvallystyrasnaspthrvalglylysvalileval
930935940
aspphelysserphelysasnileserglyvalthrpheasntrpala
945950955960
asplysproproileasnvallysleuserlystyrserglyaspgln
965970975
phethrserilevalaspphephealalysvalasppheglyasnglu
980985990
leutyrasnsertyrlystyralaasnprogluasphisilepheasn
99510001005
glnsergluvalpheglugluilehissergluservalthrile
101010151020
seralapropheaspasnlysglutyrthrglnvalleuvalpro
102510301035
thrarghisasnthrthrserileaspleuasnleuserthrarg
104010451050
pheleuleuilegluvalaspasnilehisasnthrvalproile
105510601065
aspaspasptyrglyglyalalysleualagluvalvalphetyr
107010751080
<210>55
<211>1078
<212>prt
<213>白假丝酵母(candidaalbicans)
<400>55
metalaalaasnserserphepheleualaaspasncysalaprohis
151015
asnglnserpheileglnphecysilehisalaalaserlyslyslys
202530
glytrpilealaleumetcysleualaasnleupheleuleupheser
354045
phehisleuleutyralaargphecysserglypheprotyrvalval
505560
proserthralaargserthrasnglnilephehisthrasnleuglu
65707580
glnleuvalasnserprogluasnlysglnilepheserglnleulys
859095
pheseraspglnalaphetyraspprohisaspasnvalvalglythr
100105110
thrglupheprovalpheasnglntyrglnargglnprotyrvalala
115120125
asnglytyrileglyserargileproasnleuglyglnglyphethr
130135140
tyraspglnleuthrasnserserthralaasnaspaspaspleuleu
145150155160
asnglytrpproleupheasnlysargtyrserglyalaphevalala
165170175
glyphetyraspleuglnlysasnthrthrglythrasnphealaglu
180185190
leuleugluasnglytyrgluservalilealaalavalproglntrp
195200205
thralaleuserleuservalgluileleuglylyslystyrthrleu
210215220
aspproserleugluhisglualaileglyaspilethrasntyrval
225230235240
glnasnmetserleuseraspglyilevalthrthrglnphethrtrp
245250255
leuasnthrpheaspvallystyrgluileleualahisarggluasn
260265270
ileasnleuglyleuvalasnmetglnvaltyrasnproglyasnglu
275280285
servalglnvalilevalseraspvalleuasppheasnsersergln
290295300
argcysglnleuasnglnileserhisasplysasnglyiletyrval
305310315320
thrphehisproglnglyleusertyrileaspglyalailetyrser
325330335
thrleuseralaasnglyglnilethrarggluglnthrasngluthr
340345350
valpheglnasnvalgluleuthrilegluprohissercysvalgln
355360365
valalalystyrvalglyilealathrthraspleuaspproaspser
370375380
phelysthralaaspaspvalleulysphealaarglysvalsergln
385390395400
asnlyslyshisglyaspalathrglnleuvalasnserhisargser
405410415
alatrpserlysileileglnaspalaproleuvalthrpheproser
420425430
aspserleuleuasnleuglyalaargalaserilephehisleuleu
435440445
alaasnthrargproasnalagluglyvalthrglyalaleuglyval
450455460
serglyleuserseraspsertyrglyglymetvalphetrpaspthr
465470475480
aspleutrpmetleuasnglyileleuprophealaproasphisile
485490495
lysserpheileasntyrargvalhisleuhisglnglnalaileasp
500505510
asnvalproargglytyrglnglyalavaltyrprotrpthrsergly
515520525
argpheglyasncysthrglythrglyprocysleuasptyrglutyr
530535540
hisileasnmetalavalalametalasertrpglnleutyrileser
545550555560
glyalaalaaspaspthrpheleugluservalalatyrproileile
565570575
asnaspalaalaserpheleualaglutyrvalvalhistyrasnasp
580585590
thrleuglylystyrthrthrlysasnleuthraspproaspgluphe
595600605
alaasnhisvalaspasnglyalatyrthrasnthrglyilevalleu
610615620
valmetargtrpalaglnthralaglyserileleuglylysglnval
625630635640
prolysiletyrhisaspilegluthralametpheleuprothrala
645650655
gluasnthrglnasnilethrleuglutyrserglymetasnserser
660665670
valglyilelysglnalaaspvalilemetmetthrtyrproleuglu
675680685
asngluleuileaspglnaspglnalatyrileasnmetgluphetyr
690695700
sermetlysglnvalglytyrglyproalametthrpheproilephe
705710715720
serilevalalaserasnleualaphethrglycysalaserglnser
725730735
tyrleuhislysalaileglnpropheleuargglyprophealagln
740745750
phealagluglnasnasnaspasptyrleuthrasnglyglythrhis
755760765
proalaphepropheleuthralahisglyglypheleuglnalaile
770775780
leuglnglyleuthrglymetargpheasptyrthrpheaspasnasn
785790795800
lysleuglnargleuleulysleuaspproilealaleuprocysleu
805810815
glygluglyvalargpheaspserilelystyraspasnhisthrleu
820825830
sermetalaileasngluthrhisphethrilelysasnlysglylys
835840845
thrthrproasnalaargasntyrvalthrileleuleualagluarg
850855860
asnalamethisglylystyrthrileasnaspgluaspgluglnser
865870875880
pheproleuphegluthrsergluserpheproaspserileserglu
885890895
cysasnlysalaglyphepheasnilethrgluglyalatyrglyasp
900905910
valserileserileasnaspglyaspasnthrthrsertrpglnala
915920925
lystyrasnaspthrthrglylysvalleuvalaspleulysserphe
930935940
argasnileserserglythrpheiletrpglyasplysproprolys
945950955960
argvallysvalserlystyrserglyserserphethralavalthr
965970975
aspphephealaglnvalasppheglyasngluleupheasnglutyr
980985990
lystyralaasnprogluglylysleuhisasnglnseraspvalphe
99510001005
glugluvaltyrserglyaspvallysileseralapropheasp
101010151020
progluglutyrpheglnvaltrpvalprothrarghisasnile
102510301035
thrgluvalalavalasnleuglnthrargpheleuleuileglu
104010451050
valaspgluilehisasnthrglualaileaspglyasptyrgly
105510601065
glyalalysleualagluvalvalphetyr
10701075
<210>56
<211>1140
<212>prt
<213>长孢洛德酵母(lodderomyceselongisporus)
<400>56
metvalserasnglugluaspiletyrileasnhisaspproasnpro
151015
asnpheasnseraspserasnserasnserasnseraspproasppro
202530
asntyrasnpheileserargphethrserthrproserileasnser
354045
aspserhisleuglyserhisproasnserthrpheglnleutyrleu
505560
asnargalalysglylyssersermetileilemetalaleuileala
65707580
asnpheileleuphetyrserleuhisleuleuasnvalargphecys
859095
glualapheprotyrleuvalprogluvalglulysgluservalphe
100105110
asnasnilepheserserleugluglyleugluaspleuvalasnser
115120125
tyrgluasnlysaspilepheserglnleulystyrserglyglnala
130135140
phetyraspasngluleuasnvalvalglythrleuglupheproleu
145150155160
tyrasnlystyrglnargglnprotyrvalalaasnglytyrilegly
165170175
serargileproasnleuglyhisglyphethrtyraspglnleupro
180185190
aspthrcysglntyrcysgluaspaspleuserasnglytrpproleu
195200205
pheasnlysargphealaglyalapheilealaglyphetyraspleu
210215220
glngluasnthrthrglythrasnpheprogluleuleugluasngly
225230235240
tyrgluservalilealaalavalproglntrpthrthrleuasnval
245250255
alaileglualaasnglyglnasntyrthrleuaspproserleuglu
260265270
thrhisgluileglyasnilethrasntyrvalglnasnmetserleu
275280285
serasnglyilevalthrthrglntyrthrtrpleuhisasnileasp
290295300
ilelystyrthrvalvalalahisargalaglnproasnleuglyleu
305310315320
valthrleuaspileglnserargseraspgluvalvalasnleuarg
325330335
valgluaspileleuasppheasnthrserglnargcysgluleuthr
340345350
alaleuglyhisaspgluleuglyiletyrilethrpheglnproglu
355360365
asnvalaspseralahisglyalailetyrserargleuilegluser
370375380
leugluseralaglyalathrglyalaseralavalserasnleuglu
385390395400
lysvalseraspglygluargileserglnilealalysilethrleu
405410415
gluprophegluserthrglnileasnlysvalvalglyilevalser
420425430
seraspseraspprogluasnleuglythralalysservalargglu
435440445
seralalysglualaalaglnlystyrasnasnileglnaspileleu
450455460
thrthrhisasnaspalatrpthrlysleuleuglygluasnproarg
465470475480
valthrpheprosergluproleuleuasnleualaalaargalaser
485490495
valtyrhisleuleualaasnthrargproglnalapheglyleuthr
500505510
glyalavalglyvalglyglyleuserseraspsertyralaglymet
515520525
valphetrpaspthraspleutrpmetpheasnglyileleuproleu
530535540
metproserhisalalysserilevalasntyrargleuhisleuhis
545550555560
gluglnalavalglnasnvalprogluglytyrasnglyalavaltyr
565570575
protrpthrserglyargtyrglyasncysthralathrglyprocys
580585590
leuasntyrglutyrhisileasnthralavalalametalaalatrp
595600605
gluleutyrileserglyalaseraspgluleupheleuargglutyr
610615620
alaserthrleuileasnasnalaalathrphepheserasptyrleu
625630635640
valglntrpasnaspthrleuglyglntyrthrthrserasnleuthr
645650655
aspproaspglutyralaasnhisvalaspasnglyalatyrthrasn
660665670
serglyileserleuleumetglntrpalathrmetvalglyhisile
675680685
leuglygluaspvalproaspvaltyrserglnilealagluasnleu
690695700
hisileproseralaasnasnserglnasnilethrleuglutyrser
705710715720
glymetasnserthrvalglyilelysglnalaaspvalilemetmet
725730735
thrtyrproleualaasnasnleuileaspglugluglnglytyrile
740745750
asnmetglutyrtyrsermetargglnvalsertyrglyproalamet
755760765
thrpheproilepheserilevalalaserlysleualavalsergly
770775780
cysalaserglnsertyrleuhislysalavalglnprotyrleuarg
785790795800
glyprophealaglnphesergluglnasnasnaspasptyrlysthr
805810815
asnglyglythrhisproalaphepropheleuthralahisglygly
820825830
pheleuglnalaalaleuhisglyleuthrglymetarghisargleu
835840845
asnvalseraspthrglyvalilegluargpheleupheleuasppro
850855860
ilealaleuprocysleuglygluglyvalleupheasnservalphe
865870875880
tyrasnasnhisthrilesermetserileasnglnthrhisphethr
885890895
ilegluasnasnglylysthrseraspserglnasnalaaspglyphe
900905910
ilethrleuglnleualagluargasnproasphisglythrpheval
915920925
leuleuaspglyglulysargserphealaleutyrlysproglulys
930935940
serphevalaspserileserglucysglyaspalaserphetyrasn
945950955960
ilethrgluglyalatyrglyvalthrproileserileasnaspgly
965970975
aspasnthrthrargtrpglnalalysthrasnaspthrvalglylys
980985990
ileleuvalaspleuhisserilelysasnvalserservalleuphe
99510001005
asncysalaaspargproproleuserleulysvalserlystyr
101010151020
ileglyasplysphethrglnvalthrasppheleuserglnval
102510301035
asppheglyasngluvaltyrlysasntyrargtyralaasnpro
104010451050
asnasplysleutyrasnglnsergluvalpheglugluvalser
105510601065
serglnaspvalserileserglupropheserglnglugluphe
107010751080
aspglnvalleuvalprovalarghisasnalathrleuleuasp
108510901095
leugluileglnglncysargpheileleuilegluvalglugly
110011051110
valhisaspasnserserleuglugluaspglyaspglusertyr
111511201125
seraspglyalalysleualagluvalalaphephe
113011351140
<210>57
<211>1078
<212>prt
<213>杜氏假丝酵母(candidadubliniensis)
<400>57
metalaalaasnproserpheileleuvalaspthrargargprohis
151015
asnglnserpheileglnphecysilehisalathrserglulyslys
202530
sertrpilealaleumetcysleualaasnleuleuleuleupheser
354045
phehisleuleutyralaargleucysasnglypheprotyrvalval
505560
proserthralaargserthrasnproilehistyrthrasnleuglu
65707580
glnleuvalasnserprogluasnlysglnilepheasnglnleulys
859095
pheseraspglnalaphetyraspprohisasnasnvalvalglythr
100105110
ileglupheproleupheasnglntyrglnargglnprotyrvalthr
115120125
asnglytyrileglyserargileproasnleuglyglnglyphethr
130135140
tyraspglnleuthrasnserserthralaasnaspaspaspleuleu
145150155160
asnglytrpproleupheasnlysargtyrserglyalaphevalala
165170175
glyphetyraspleuglnlysasnthrthrglythrasnphealaglu
180185190
leuleugluasnglytyrgluservalilealaalavalproglntrp
195200205
thralaleuserleuasnvalgluleuleuglylyslystyrthrleu
210215220
aspproserleugluhisgluvalileglyasnileserasntyrval
225230235240
glnasnmetserleuseraspglyilevalthrthrglnphethrtrp
245250255
leuasnserileaspvallystyrgluvalleualahisarggluasn
260265270
ileasnleuglyleuvalasnmetglniletyrasnserglyasnglu
275280285
servalglnvalvalvalseraspvalleuaspphesersersergln
290295300
argcysglnleuasnlysvalalahisasplysasnglyiletyrval
305310315320
thrpheasnproglnglyleusertyrileaspglyalailetyrser
325330335
thrleuservalaspglyaspilethrarggluglnileasngluthr
340345350
valserglnlysalalysleuthrmetgluprohissercysvalgln
355360365
alaalalyspheileglyilealathrthrasppheaspproaspser
370375380
phelysasnalaaspaspvalleulysphealaarggluvalsergln
385390395400
asnlysasnhisglyaspalaserglnleuileserserhisargser
405410415
alatrpserlysileileglnaspthrproleuilethrpheproser
420425430
aspproleuleuasnleuglyalaargalaserilephehisleuleu
435440445
alaasnthrargproasnalagluglyvalthrglyalaleuglyval
450455460
serglyleuserseraspsertyrglyglymetvalphetrpaspthr
465470475480
aspleutrpmetleuasnglyileleuprophealaproasphisile
485490495
lysserpheileasntyrargvalhisleuhisglnglnalaileasp
500505510
asnvalproserglytyrglnglyalavaltyrprotrpthrsergly
515520525
argpheglyasncysthrglythrglyprocysleuasptyrglutyr
530535540
hisileasnmetalavalalametalasertrpglnleutyrvalser
545550555560
glyalaalaaspaspthrpheleugluservalalatyrproileile
565570575
asnaspalaalaserpheleualaglutyrvalvalhistyrasnasp
580585590
thrleuglylystyrthrthrlysasnleuthraspproaspgluphe
595600605
alaasnhisvalaspasnglyalatyrthrasnthrglyilevalleu
610615620
valmetargtrpalaglnmetvalglyargileleuglylysgluval
625630635640
proargvalphehisaspilegluthralametpheleuprothrala
645650655
gluasnserglnasnilethrleuglutyrserglymetasnserser
660665670
valglyilelysglnalaaspvalilemetmetthrtyrproleuglu
675680685
asngluleuileasplysaspglnalatyrileasnmetgluphetyr
690695700
sermetlysglnvalglytyrglyproalametthrpheproilephe
705710715720
serilevalalaalaasnleualaphethrglycysalaserglnser
725730735
tyrleuhislysalaileglnpropheleuargglyprophealagln
740745750
phealagluglnasnasnaspasntyrleuthrasnglyglythrhis
755760765
proalaphepropheleuthralahisglyglypheleuglnalaile
770775780
leuglnglyilethrglymetargpheasptyrthrphegluaspasn
785790795800
argleuglnargileleuthrleuaspproilealaleuprocysleu
805810815
glyglyglyvalargpheaspglyilelyspheaspasnhisthrleu
820825830
sermetalaileasngluthrhisphealaileargasnasnglylys
835840845
alathrproasnalaglnasnhisvalthrileleuleualagluarg
850855860
asnpromethisglylystyrthrileasnasplysaspgluleuser
865870875880
pheproleuphegluthrsergluserpheproaspservalserglu
885890895
cysserlysalaglyphepheasnilethrgluglyalatyrglyasp
900905910
valserileserileasnaspglyaspasnthrthrsertrpglnthr
915920925
lystyrasnaspthrthrglylysvalleuvalaspleulysserleu
930935940
lysasnileserserglythrpheiletrpglyasplysproprolys
945950955960
argleulysileserlystyrserglyaspglyphealathrvalthr
965970975
aspphephealaglnvalasppheglyasnlysleupheasnglutyr
980985990
lystyrthrasnprogluglylysleuhisasnglnseraspvalphe
99510001005
glugluvaltyrserglyaspvallysileseralapropheasp
101010151020
progluglutyrpheglnvaltrpvalprothrarghisasnile
102510301035
thrgluleuthrleuasnleuglnthrargpheleuleuileglu
104010451050
valaspgluilehisasnthrgluproileaspglyasptyrgly
105510601065
glyalalysleualagluvalvalphetyr
10701075
<210>58
<211>1094
<212>prt
<213>木糖发酵酵母(spathasporapassalidarum)
<400>58
metvalarglyslysthrglntyrglnserleuasnilealaprogly
151015
proglygluthrasnasnprothrphepheglutyrleupheserser
202530
ilearghishislysglyilevalthrmetvalilephealaasnleu
354045
ilethrleuvalserilehisleuleupheleulystyralaasnala
505560
pheprohisilealaargasnglnaspglupheglythrilelysasn
65707580
asnproserlysaspglnglnserpropheseraspglnserglyleu
859095
ileasnleuileasnserproglnasnlysalailetyrserglnmet
100105110
lystyrsersertyralaphetyraspasnthrthrasnthrvalgly
115120125
thrileglutyrvalpropheasnglntyrglnargglnprotyrval
130135140
alaasnglytyrileglyserargileproasnleuglyhisglyphe
145150155160
thrtyraspglnleuseraspalaproaspalaasnaspaspaspleu
165170175
leuasnglytrpproleupheasnglnargtyralaglyalapheile
180185190
alaglyphepheaspileglnlysasnthrserglythrasnphepro
195200205
gluleuleuaspasnglytyrgluservalilealaalavalprogln
210215220
trpthrthrleulysleuserthrthrlysaspasnglnasntyrthr
225230235240
leuaspproserleuproproalaglnileglyserileserasntyr
245250255
valglnasnleuserleuserasnglyilevalthrthrglntyrthr
260265270
trpleuaspserilegluvallystyrgluileleualahisarghis
275280285
asnilehisleuglyvalvalasnleugluvaltyrasnserglyasn
290295300
gluthrvalargleuaspilethraspgluileasppheasnserala
305310315320
glnargserglnleuthrglyvalasnthraspasplysglyiletyr
325330335
valthrpheglnprosergluileasptyrvalasnglyalailetyr
340345350
serthrleuilevalaspserproilethrargglnthrthrasngly
355360365
thrthralaglnglnilealathriletyrleuglnprohisgluser
370375380
leuseralaasnlysphevalglyilealaserseraspleuasnpro
385390395400
aspglnleuthrthrthraspservalleuasnilealalysgluval
405410415
serglnlyshisglnlysleulysprovalvallysserhisasnglu
420425430
glntrplyslysthrleuglygluasnproleuvalthrpheproasp
435440445
alaproleuleuasnleuglyserargalaseriletyrhisleuile
450455460
alaasnthrargproaspalaglnglyvalthralaalaleuglyval
465470475480
glyglyleuserseraspsertyralaglyleuvalphetrpaspala
485490495
aspleutrpmetserasnalaleuleuprophealaproasphisala
500505510
lysserilevalasntyrargleutyrthrhisalaglnalavallys
515520525
asnmetproalaglytyrgluglyalavaltyrprotrpthrserala
530535540
argpheglyasncysthrglythrglyprocysleuasptyrglutyr
545550555560
hisileasnvalalavalalalysalaalatrpasntyrtyrleuser
565570575
glyalaglyaspgluasptyrleualaaspvalalatyrproileile
580585590
asnaspalaalathrphepheserglytyrleualaglntyrasnglu
595600605
servalasnargtyrthrthrhisasnleuthraspproaspglutyr
610615620
alaasnhisvalaspasnglyalatyrthrasnalaglyileserleu
625630635640
valmetlystrpalailealaleuglyasnhisleuglylysaspval
645650655
proglnilepheglnasnilealaglyasnmettyrileproserala
660665670
asnasnserglngluilethrleuglutyrserglymetasnserser
675680685
valglyilelysglnalaaspvalilemetmetthrtyrproleuasp
690695700
asngluleuleuaspleugluglnalatyrgluasnmetgluphetyr
705710715720
sermetlysglnvalsertyrglyproalametthrpheproilephe
725730735
serilevalalaalaasnleualaprothrglycysalaserglnser
740745750
tyrleuglnlysalaileglnpropheleuargglyprophealagln
755760765
phesergluglnasnasnaspasnpheleuileasnglyglythrhis
770775780
proalaphepropheleuthralahisglyglyphethrglnalaval
785790795800
leuglnglyleuthrglymetargtyrasptyrpheileglnasngly
805810815
thrleuvalargalaleuargvalaspproilealaleuprocysleu
820825830
glyserglyvalglntyrgluglyiletyrtyrasnasnhisserval
835840845
alametserileasngluthrhisphethrileglnasnargglypro
850855860
thrglulysseralaglylystyrileasnileilevalaspasparg
865870875880
asnprolysglnglyglntyralaleuaspasptyraspglumetval
885890895
ileproleutyrthrproilealasertyrproaspserileserglu
900905910
cysglyleualaserphetyrasnilethrgluglyalatyrglyasp
915920925
leuproileserileasnaspglyaspasnthrthrargtrpglnval
930935940
lysasnasnaspthrileglylysileleuvalaspleulysserphe
945950955960
lysasnileserasnileleupheasntrpargasplysproprogln
965970975
thrleuargleuservaltyrasnaspasnglnphethraspalathr
980985990
aspphepheserlysvalasppheglyasnglyleutyrasnasptyr
99510001005
lystyrhisasnproaspserglnilevalasnglnseraspval
101010151020
phethrgluvaltyrseralaservalaspilethralaprotyr
102510301035
aspserasnglupheasngluvalmetvalproaspargserasn
104010451050
ilethrserleuasnleuasnleutyrasnargpheleuleuile
105510601065
gluvalasnglyilehisaspalagluprovalgluglyaspthr
107010751080
glyglyalalysleualagluvalthrphephe
10851090
<210>59
<211>1083
<212>prt
<213>树干毕赤酵母(scheffersomycesstipitis)
<400>59
metvalvalvalserlystrpargpheseraspvalilehisthrval
151015
cysaspthrilevalaspthrphetyrarglysglnalaphevallys
202530
thrmetvalphevalasnvalglyleuileleuleuleuhistrptyr
354045
phevalserleuserglnalapheproleuglnleuargasnhisval
505560
proglythrvalthrhisgluservalalaglugluargalaarghis
65707580
leulysleuvalasnserproglnasnlysalavaltyrthrglnleu
859095
lystyralaalaasnalaphepheaspvalaspthrasnthrilegly
100105110
thrthrhisphethrprotyrasnglntyrglnargglnprotyrval
115120125
alaasnglytyrileglyalaargileproasnleuglyglnglyphe
130135140
thrtyraspglnileseraspseralaaspalathrasnaspaspleu
145150155160
leuasnglytrpproleupheasngluargtyrserglyalapheval
165170175
alaglyphepheaspileglnlysasnthrthrglythrasnphepro
180185190
gluleuleualaasnglytyrgluservalilealaservalprogln
195200205
trpthrthrleuglnvalserthrilelysaspglyvalasppheser
210215220
leuaspprovalasnservalaspmetasnasnvalilesersertyr
225230235240
glyglnasnleuserleuvalasnglyilevalthrthrglutyrthr
245250255
trpleuaspaspileargilelystyrlysileleualahisarglys
260265270
gluileasnleuglyleuvalgluleuserileserasnmetglyasn
275280285
thrserleuthrpheasnvalthraspvalleuaspalaserthrala
290295300
glnargserglnleuthrglyvalasnseraspglylysglyiletyr
305310315320
ilethrpheserproasngluleuasntyrileasnglyalailetyr
325330335
serthrleuhisvalgluaspglyserserileglnserserserser
340345350
thrserlysvalserglntyrvalgluvalthrvalasnproglyarg
355360365
thrserargvalvallysleuvalglyvalalathrthraspleuasp
370375380
proargasnleuaspserleuasplysvalleualaphealalyslys
385390395400
valserglnthrtyrthrasnalaaspaspvalvalgluserhisleu
405410415
valalatrpalaglnthrleugluserthrproalailethrpheala
420425430
aspaspargleuleuthrleualaserargalaserleuphehisleu
435440445
thralaasnthrargproaspalaasnglyvalthralaalametgly
450455460
valalaglyleuserseraspsertyralaglymetvalphetrpasp
465470475480
alaaspiletrpmetmetserglyleuleupropheileproserhis
485490495
alalysserilevalasntyrargmethisthrhisaspglnalaile
500505510
lysasnleuprogluglyalalysglyalavaltyrprotrpthrser
515520525
glyargpheglyasncysthralathrglyprocysleuasptyrglu
530535540
tyrhisileasnvalalavalalametseralatrpglnleutyrleu
545550555560
serglyalaalaaspgluglnpheleualaaspvalvaltyrproleu
565570575
valasnaspalaalagluphephealaasptyrvalvalthrtyrasp
580585590
aspthrleulysglnphethrthrhisasnleuthraspproaspglu
595600605
tyralaasnhisvalaspasnglyalatyrthrasnserglyileser
610615620
leuvalglulystrpalaileglnileserasnhisleuglylysglu
625630635640
pheproserglntyrserasnilevalglyasnmethisleuprothr
645650655
serglyasnseraspasnilethrleuglutyrthrglymetasnser
660665670
servalglyilelysglnalaaspvalilemetilethrtyrproleu
675680685
glnasngluleuileserglualaglnalaleuthrasnmetgluphe
690695700
tyrservallysglnvalasntyrglyproalametthrpheproile
705710715720
pheserilevalalaserhisvalserthrserglycysalasergln
725730735
sertyrleuglnlysalavalglnpropheleuargglypropheala
740745750
glnphesergluglnasnasnaspasppheleuthrasnglyglythr
755760765
hisproalapheprophemetthralahisglyglypheleuglnala
770775780
valthrglnglyleuthrglyleuargpheglytyrvalilegluasp
785790795800
glyglnilelysargalaleuaspleuaspprothralaleuprocys
805810815
leuproasnglyvalilepheaspglyilelystyrasnasnhisser
820825830
leuserphealavalasngluthrserphethrvallysasnasngly
835840845
proileserglulysseraspglyvalvalargileargilealaasp
850855860
argasnproserargglyiletyrthrileasnserglygluaspphe
865870875880
serpheproleutyrthrprolysprosertyrprothrserileser
885890895
glucysglyleualaserphetyrasnilethraspglyalatyrgly
900905910
aspserproileserileasnaspglyaspasnthrthrglntrpgln
915920925
alalystyrasnaspthrthrglylysileleuvalaspphelysgln
930935940
phelysasnvalserasnglyileileasntrpglyasplyspropro
945950955960
lysasntrplysleuserglnphethrglyserleuvalgluphelys
965970975
aspvalgluaspvalleuserglnvalasppheglyasngluleutyr
980985990
asniletyrargtyrgluaspgluasptyrlysleutyrlysglnasp
99510001005
gluvalpheglnvalvalleuserserasnvalserileserala
101010151020
propheileleugluasptyrasnthrilegluleuprolysarg
102510301035
glnasnthrthrgluphethrileaspglugluleutyrsergln
104010451050
pheleuleuilegluilegluglyilehisasnthrvalproile
105510601065
gluaspaspthrglyglyalalysleutyrgluvalvalphephe
107010751080
<210>60
<211>1101
<212>prt
<213>法布里德巴利酵母(debaryomycesfabryi)
<400>60
metglutyraspserleuglygluargphelyshisargalaileser
151015
lysvalasphiscysasnhispheilehislysphecyslysarggln
202530
ilevalilelysthrmetilevalleuasnpheileleuleupheval
354045
leutyrleutyrhisglnglyilethrtyralaleuprophealaile
505560
lysaspvalasnileaspaspserpheasnaspserthrvalthrphe
65707580
gluthrilegluserasnarglysglnleuglnlysleuileasnser
859095
arggluasnlysgluilepheleuglnleuglnasnserglyserala
100105110
tyrtyraspprothrthrasnthrvalglythralaglupheprothr
115120125
tyrasnglntyrglnargglnalatyrvalserasnglytyrilegly
130135140
serargileproasnleuglyglnglyphethrpheaspglnleuser
145150155160
aspserproaspalavalgluaspaspleuserasnglytrpproleu
165170175
pheasngluargpheserglyserpheileglyglyphetyraspile
180185190
glnlysasnthrthrgluthrasnpheprogluleuilelyslysgly
195200205
tyrgluserileleuseralavalproglntrpthrthrleuthrleu
210215220
serthrvallysasnglylysthrpheserleuaspproserleuser
225230235240
argaspalaglnglyaspileserasntyralaglnasnleuserleu
245250255
serasnglyilevalthrthrgluphethrtrpleuaspserleugln
260265270
valhisilegluvalvalalahisargserasnileasnleuglyile
275280285
valasnleuargilevalasnleuglyasnserthrvalaspleulys
290295300
valgluasplysleugluphealaserthrglnargcysglnleuthr
305310315320
gluvalglyhisaspaspgluglyilepheilehispheglnproasn
325330335
gluileasptyrvalasnglyalailetyrserthrleulystyrasp
340345350
glulysserleuasnserileserargaspserlysasnaspthrser
355360365
thrglngluleuaspileservalgluproserlysserphelysile
370375380
serlysmetvalglyilevalserseraspleuaspproglulystyr
385390395400
lysthrserserservalasnaspphealalysglnvalalathrlys
405410415
glnalaaspservalseraspleuilelysserhislysthrglutrp
420425430
aspargthrphegluserserasnserilethrpheproglyaspser
435440445
leuleuthrleualaserargalaseriletyrhisleuasnalaasn
450455460
thrargproglyalaargglyvalthralaalaleuprovalglygly
465470475480
leuserseraspsertyrglyglymetvalphetrpaspthraspleu
485490495
trpmetleuasnglyleuleupropheasnproasphisalalysser
500505510
ilevalasntyrargilehisthrhisgluglnalalyslysasnval
515520525
proaspasnlysglyglyalavaltyrsertrpthrserglyargphe
530535540
glyasncysthrserthrglyprocysmetasptyrglutyrhisile
545550555560
asnilealavalalametalaalatrpgluvaltyrleuserglyala
565570575
alaaspaspasptyrleuaspservalalatyrproleuileasnasp
580585590
alaalathrpheleualaasntyrvalglutyrasnaspserleuala
595600605
glntyrvalthrhisasnmetthraspproaspglutyralaasnhis
610615620
valaspasnalaalatyrthrasnalaglyileserleuleumetlys
625630635640
trpalailethrileserasnhisleuglylysservalprothrlys
645650655
tyrthraspilealaglysermethisileprothrseraspasnala
660665670
aspasnilethrleuglutyrserglymetasnserservalglyile
675680685
lysglnalaaspvalilemetmetthrtyrproleuglyasngluleu
690695700
ileseraspaspglnalatyrthrasnmetgluphetyrsermetlys
705710715720
glnvalsertyrglyproalametthrpheproilepheserileval
725730735
alaserasnleuserproserglycysalaserglnsertyrleuhis
740745750
lysalavalglnpropheleuargglyprophealaglnpheserglu
755760765
glnasnasnaspasnpheleuthrasnglyglythrhisproalaphe
770775780
prophemetthralahisglyglypheleuglnalaileleuglngly
785790795800
leuthrglyleuargpheasptyrasnileaspaspthrasnlysleu
805810815
serargmetleuthrleuaspproileserleuprocysleuglyasn
820825830
glyvalglnpheaspserilelystyrmetasnlysthrleuserleu
835840845
alaileasngluthrserpheilevallysasnasnglyproilethr
850855860
glyserlysaspaspserproilelysileserleualalysargasn
865870875880
prolysalaglyvaltyrthrleulyslysglyglugluleuvalphe
885890895
proleuphegluproglualaglyserproleuservalserglucys
900905910
alaseralalyspheileasnilethrgluseralatyrglyaspala
915920925
thrvalleuvalasnaspglyaspasnthrthrhistrpglnleulys
930935940
tyrasnaspthrthralalysleuleuvalaspleulysglnproarg
945950955960
asnilethrserglyvalileasntrpglyaspargproprolysser
965970975
trpserleuleualatyrglyserhisgluglnserlysilelysasp
980985990
leuasnaspasnileasppheleuserlysvalasnpheglyasnasn
99510001005
leutyrlyslystyrgluphemetaspvalaspmetvaltyrasp
101010151020
glnaspaspvalphethrthrileilesergluservalaspile
102510301035
seralaprotyraspprolysgluseralaglnilegluilepro
104010451050
leuarghisasnthrthrserphealavalgluglnglyleuasn
105510601065
alaargpheleuleuilegluvalthraspilehisaspthrglu
107010751080
proileaspaspgluthrglyglyalalysleutyrgluvalglu
108510901095
phepheglu
1100
<210>61
<211>1049
<212>prt
<213>坦扎瓦丝假丝酵母(candidatanzawaensis)
<400>61
metmetilevalalaasnleualapheleuleuvalleuglnleuthr
151015
trpaspvalleutrpalahisproleuthrvalprolysleupheala
202530
alaserasnleuseralaaspthrvalhisthraspargaspalaleu
354045
phealaleuileasnserthralaasnlysglnvalpheserglnleu
505560
lystyrserserseralatyrtyraspleuaspalaasnilevalgly
65707580
seralagluphealaproasnasnglntyrglnargglnprotyrval
859095
alaasnglytyrileglyalaargileproasnleuglyglnglyphe
100105110
thrtyraspglnleuserthraspalaaspalathraspalaaspleu
115120125
leuasnglytrpproleutyrasnlysargtyralaglyalapheile
130135140
alaglyphetyraspileglnalaasnthrthrgluthrasnphepro
145150155160
gluleuglulysasnglytyrgluserileilealaalavalprogln
165170175
trpthralaleugluleuserthrthrvalaspglyvalasntyrthr
180185190
leuasnproalaleugluserhisleuglnglyserileserasntyr
195200205
alaglnasnleuserleuseraspglyilevalserthrgluphethr
210215220
trpleuaspservalglnvallysilegluileleualahisarghis
225230235240
hisvalasnleuglyvalvalhisleugluleualaasnileglyser
245250255
seralaleuaspilelysvalargaspvalleuaspphealathrala
260265270
glnargserglnleualaservalasphisaspglnaspglyiletyr
275280285
ilethrpheglnproasngluileasptyrvalasnglyalailetyr
290295300
serthrleuleuserasphisproproasnphegluglnalathrasn
305310315320
glythrvalalaglnserleuaspileglnleuglnproglyserthr
325330335
phelysvalthrlystyralaglyilevalserseraspleuasppro
340345350
alaasnleulysserglulysaspvalleuserleualaargargval
355360365
serglnserphethraspilelysgluvalphegluthrhislysthr
370375380
alatrpalaglnthrleugluserthrproalavalthrpheproasn
385390395400
aspleuleuleuthrilealaalaargalaseriletyrhisleuala
405410415
alaasnthrargthraspalagluglyvalthralaalaleuproval
420425430
valglyleuserseraspsertyrglyglymetvalphetrpaspthr
435440445
aspleutrpmetpheasnglyleuleupropheleuproserhisala
450455460
lysserleuileasntyrargilehisthrhisgluglnalalyslys
465470475480
asnvalproalaglytyrgluglyalaalatyrprotrpthrsergly
485490495
argpheglyasncysthrserthrglyprocysvalasptyrglutyr
500505510
hisileasnmetalavalalaserseralatrpasnleutyrleuser
515520525
glygluvalglygluasppheleugluservalalatyrproleuile
530535540
lysaspalaalathrphepheserglutyrvalvallysleuaspglu
545550555560
serthrglylystyrvalthrhisasnleuthraspproaspglutyr
565570575
alaasnhisvalaspasnglyalatyrthrasnalaglyileserleu
580585590
valmetlystrpalaileaspvalserasnhisleuglylysgluval
595600605
proserglnphemetaspileseraspasnvalleumetprothrser
610615620
asnasnseraspglnilethrleuglutyrserglymetasnalathr
625630635640
valglyilelysglnalaaspvalilemetvalasntyrproleuglu
645650655
asngluleuilethrprogluglnalaleulysasnmetgluphetyr
660665670
serlyslysglnvalsertyrglyproalametthrpheproilephe
675680685
serilevalalaserasnvalserproserglycysalaserglnser
690695700
tyrleuarglysalavalglnpropheleuargglyprophealagln
705710715720
phesergluglnasnasnaspasppheleuthrasnglyglythrhis
725730735
proalapheprophemetthralahisglyglypheleuglnalaile
740745750
metglnglyleuthrglyleuargtyraspphealavallyshisasn
755760765
lysileglnargvalleulysleuaspproilealaileprocysleu
770775780
glyglyglyvalphetyraspglyilehispheaspasnhisthrleu
785790795800
thrmetserileasngluthrtyrphethrvallysasnasnglypro
805810815
alaasngluphealasergluserilegluileargvalglygluarg
820825830
asnlysglualaglyglnhisthrleulysasnglygluthrleuthr
835840845
ileproilephethrproglyglusertyrlysalaserilethrglu
850855860
cysalaleuseraspphevalasnilethralaglyalatyrglyasp
865870875880
alathrvalleumetasnaspglyaspasnthrthrasntrpglnval
885890895
alaserasnthrservalglylysvalleuileaspleulysglutyr
900905910
lysasnvalthrlysglyphepheasntrpglyasplysproprolys
915920925
glnvallysleuseralatyrasnserleuhisaspleuglnasnthr
930935940
thralapheleuserlysileasppheglyasngluvaltyrthrasn
945950955960
tyralatyralaasnproserglygluleuilelysglnglugluval
965970975
phegluserpheleuglugluservalaspvalthrvalprophethr
980985990
metaspasppheleuserleuthrvalprovalvalpheasnthrthr
99510001005
thrphegluleuproasnprovalphethrargphevalleuleu
101010151020
gluleuglnglyilehisaspthrgluproileaspleuaspthr
102510301035
serglyalalysvaltyrglualavalleutyr
10401045
<210>62
<211>1147
<212>prt
<213>多布克鲁维酵母(kluyveromycesdobzhanskii)
<400>62
metmetleuileleuproleuleuleupheservalthrileleuala
151015
provaltyrphetyrleuserlysprometproleuglnhisproleu
202530
pheglyserilevalcysvalaspglugluleutyrcysprogluser
354045
lysarglysalaserglulysmettyrserleuleulysaspserasp
505560
histhrphetyraspglugluglnglnvalleuglyasnleuleupro
65707580
sergluasnthrpheserargglnprotyrvalalaasnglytyrile
859095
glyserargileproasnleuglypheglytyralaleuaspthrmet
100105110
asnvaltrpvalasnaspserserileproglyalaleuaspasngly
115120125
trpproleuargasnargargphealaglyalaphevalseraspphe
130135140
tyrcysleuglnglulysleuasnserthrasnpheprogluleuasp
145150155160
aspasnglytyrserthrvalileserthrileproglntrpthrasp
165170175
leuserileilearghisthraspleuglygluvalglutyrileasn
180185190
prothraspvalasnleuasplysileserasntyrmetglnasnleu
195200205
serleuglnaspglyilevalthrthrserphevaltyraspasnser
210215220
leuleuvalthrthrlysvalilealahisarglysiletyrproleu
225230235240
glyilevalthrleugluleuserservalglyasnasnglupheser
245250255
aspglyleuhisserlysservalgluleugluilecysaspthrleu
260265270
asnpheserthrserhisargthrvalleualaasphisglytyrasp
275280285
arglysasnasnglyilephemetvalvalgluprogluasnvalpro
290295300
tyrserasnalaserleuphesertyrpheaspilealaserargasp
305310315320
asnleuthrphethrlysserproasnserileasnglncysthrlys
325330335
hisvalleuthrproasnthrthrphevalalahislystyrvalgly
340345350
ileileserserglutyraspasnglnalaserthrvalvalthrasn
355360365
lysthraspaspasnleutyrglumetserasnleugluargalaile
370375380
serilevalserserasnglnglyasntyrgluserleuileglnleu
385390395400
hisarggluthrtrpglulysiletyrlysaspalaserilegluile
405410415
proseraspargleuleuglumetthralaargserseriletyrhis
420425430
leuleualaasnthrargserhisasnileserlysaspargglyleu
435440445
proleuglyvalserglyleuserseraspsertyrglyglymetval
450455460
phetrpaspseraspleutrpileleuproserleuleuprophephe
465470475480
progluthralalysglnileasnasntyrargasnalaserleuser
485490495
glnalalysleuasnalaglnglntyrglytyrgluglyalavaltyr
500505510
protrpthrserglyargphealaasncysthrserthrglyprocys
515520525
valasptyrglutyrhisileasnvalaspilealaleuserserphe
530535540
alailetyrmetasnglyglyvalaspaspgluglusergluglutyr
545550555560
leuargtyrthrthrtrpprophevalgluasnalaalalysleuphe
565570575
thrglntyrvallystrpasnaspsermetglnglntyrthrthrhis
580585590
asnleuthraspproaspgluphealaasnpheileaspasnalaala
595600605
phethrasnalaglyileglnthrilemetvaltrpalathraspile
610615620
alaarghisleuglyilegluproaspprolystrpleuasnileala
625630635640
aspasnilehisileproileseraspserasnilethrleuglutyr
645650655
serglymetasnserserileaspilelysglnalaaspvalvalleu
660665670
metiletyrproleusertyrilethraspglnserileleuasnasn
675680685
alailelysasnleutyrtyrtyrsergluargglnseralasergly
690695700
proalametthrtyrprovalphevalalaglyalaalaserleuleu
705710715720
asnhisglycysserserglnsertyrleutyrlysservalvalpro
725730735
tyrleuargserprophealaglnphesergluglnseraspaspasn
740745750
pheleuthrasnglyleuthrglnproalaphepropheleuthrala
755760765
asnglyglypheleuglnserileleupheglyleuthrglyleuarg
770775780
tyrsertyrglupheaspgluglualaglulysileasnargleuleu
785790795800
lyspheasnproileglnleuproleuleuproglyglyileargile
805810815
asnasnphelystyrmetglyglnvalleuaspvalleuilethrasp
820825830
thrgluglyileileilehislyslysglyasnlysaspilelysile
835840845
lysvalproaspargalaleuileproaspilegluilegluasnpro
850855860
serglualaaspproilelysglnilemethisglyargargserser
865870875880
hisasnasnlysasntyrphethrilelysproglyaspvalphelys
885890895
thrproleutyrileprolyslysasnilegluglyasnleuvalglu
900905910
alalysglnilethrasnleuthrgluglyvalproglyaspvalala
915920925
valserileleuaspglyasnasnphethrhistrpglnproalatyr
930935940
lyslysleuproalalysleuleuileaspleuglyasnasnthrglu
945950955960
aspaspilevalalaglylysileleutrpglyserargproalalys
965970975
serpheserleuserileleuproglnthrglugluilealaglnasn
980985990
leuthrhisileleuserasnvalasnglntyrcysasngluserser
99510001005
aspthrcysgluarglysleuglnasnprolysgluasppheasp
101010151020
alaalaileileaspilepheasptrptyrglyileaspleuasp
102510301035
alailearglyslyshisprogluleuglygluilelysthrlys
104010451050
phevallysleuleuaspasntyrthrvalthrproserglupro
105510601065
tyrprotrpargvaltyrasngluserlysilevalleuleupro
107010751080
glyasngluthraspphethrileasptyrserlysvalproasp
108510901095
leuasnlysaspasngluaspileasppheargseraspgluthr
110011051110
asntrplyslysproargpheilevalleuthrvalhisaspthr
111511201125
tyraspasnaspaspasnglulysglyalathrilelysgluphe
113011351140
alaleupheser
1145
<210>63
<211>1157
<212>prt
<213>马克斯克鲁维酵母(kluyveromycesmarxianus)
<400>63
metileileileproleuvalvalleuvalphethrvalleualapro
151015
valtyrphetyrvalthrlysprogluserserthrhisserleuphe
202530
progluleualaproalaargilesertrpprophealaglythrcys
354045
alaserserserglyglyglugluaspproleutyrcysproaspala
505560
tyrarglysalaserglulysmettyraspleuleulysaspasnglu
65707580
tyralaphetyraspgluthrsergluthrleuglyasnleuleuleu
859095
sergluasnthrpheserargglnprotyrvalalaasnglytyrile
100105110
glyserargileproasnvalglypheglyphealatyraspalaile
115120125
asniletrpvalasnaspseralaileproglyalaleuasnasngly
130135140
trpproleuargasnglnargtyralaglyserphevalseraspphe
145150155160
tyrserleuglnglulysleuasnserthrasnphealagluleuasp
165170175
lysaspglytyrserthrvalileserserileproasptrpthrasp
180185190
leuserilemetilehisargglyproglygluasnasnvalglutyr
195200205
ileasnprothraspvallysleuasplysilethrasptyrmetgln
210215220
asnleusermetargaspglyilevalthrthrlysphevaltyrasp
225230235240
asnasnleuphevalthrthrargthrleualahisargseriletyr
245250255
proleuglyilevalaspmetgluilegluleuleuproglnalathr
260265270
gluasnglyleuhisglualaservalgluleugluilecysaspthr
275280285
pheasnphethrthrserhisargthrvalleualaasppheglyhis
290295300
asplyslysasngluglyiletyrmetilevalgluprogluasnval
305310315320
protyrserasnalasermetphesertyrpheaspileproserarg
325330335
aspglutyrthrvalalalysthrasnaspservalserglncysthr
340345350
argargvalleuthrthraspserarggluasnserthrpheileval
355360365
arglysphethrglyilevalserserglutyraspasnasnasnpro
370375380
gluhismetserasnleugluargalathralavalvalmetgluasn
385390395400
lysglyasptyrlysasnleuleulysmethisargasphistrplys
405410415
argleutyralaaspalaserilegluileproseraspglyleuleu
420425430
glumetthralalysserseriletyrhisleuleualaasnserarg
435440445
serhisasnvalserglnserargglyleuprovalproprosergly
450455460
leuserseraspsertyrglyglymetvalphetrpaspalaaspval
465470475480
trpmetleuproalaleuleuprophepheprogluilealalysgln
485490495
metseralatyrargasnalaserleualaglnalalysgluasnala
500505510
lyslystyrglyleuglnglyalailepheprotrpthrserglyarg
515520525
phealaasncysthrserthrglyprocysvalasptyrglutyrhis
530535540
ileasnvalaspilealaleuserserleutyriletyrmetsergly
545550555560
glugluaspgluglulyssergluglutyrleuargtyrthrthrtrp
565570575
propheilegluasnalaalalysmetphethrasptyrvallystrp
580585590
asnaspthrleuglnglntyrthrthrhisasnleuthraspproasp
595600605
gluphealaasnhisvalaspasnglyalaphethrasnalaglyile
610615620
lysserilemetglytrpalahisaspilealaasnhisleuglyleu
625630635640
aspproaspprolystrpthrgluilealaglulysilehisilepro
645650655
ileseraspthrasnilethrleuglutyrthrglymetasnserser
660665670
valaspilelysglnalaaspvalvalleumetthrtyrproleugly
675680685
tyrphethrgluthrserglnproargasnalailelysaspiletyr
690695700
tyrtyrsergluargglnseralaserglyproalametthrtyrpro
705710715720
valphevalalaalaseralaserleuleuasnserglyserserser
725730735
glnsertyrleutyrlysservalvalprotyrleuargserprophe
740745750
alaglnphesergluglnseraspaspasnpheleuthrasnglyleu
755760765
thrglnproalaphepropheleuthralaasnglyglytyrleugln
770775780
serileleupheglyleuthrglyleuargtyrsertyrgluvalasp
785790795800
lysaspthrglylysmethisargleuleulyspheasnproileser
805810815
leuprometpheproglyglyileargileasnasnphelystyrmet
820825830
glyglnvalleuaspileleuleuthraspasngluglyileilelys
835840845
hislyslysglyasnlysserileleuilelysileproasparggly
850855860
aspileproaspvallysproaspglutyrthrglnileasnglythr
865870875880
servalasnvallysargalavalproserglyglusertyrhisthr
885890895
ilegluproglythrvalphelysthrproleutyrasnprolysarg
900905910
asnmetalaasnasnilevalgluserlysargalathrasnilethr
915920925
valglyvalproglyaspvalalavalseralaileaspglyasnasn
930935940
tyrthrhistrpglnproalaasnlyslysglnproglyargileleu
945950955960
ileaspmetglyasnglythralaasngluilelysserglylysile
965970975
leutrpglyasnargproalalysserpheserleuserileleupro
980985990
glnpheaspglnilethrglnasnmetthrservalleuserglnpro
99510001005
serserhisasncysserasnaspaspglytrpaspserasncys
101010151020
lystyrglnglugluglugluasnileaspalaalailelysasp
102510301035
valpheglutrptyrglymetaspleuglnservalilegluasn
104010451050
tyrprogluleuserasnvalsermetglypheilelysleuval
105510601065
asphistyrasnvalthrprosertyrprotrplysasnvalasn
107010751080
serthrargilegluleuthrleuglyasngluthrasnpheval
108510901095
valasptyrserlysvalprogluleuasnleuasnasnasnleu
110011051110
glyvalaspleuglnserlysaspthrargtrparglysproarg
111511201125
phevalvalleuthrvalpheaspthrtyraspaspaspaspglu
113011351140
vallysglyalathrilelysgluleuserleupheaspasn
114511501155
<210>64
<211>1169
<212>prt
<213>鲁氏接合酵母(zygosaccharomycesrouxii)
<400>64
metmetlysileaspleuasnalaaspproglyaspileserglnthr
151015
proglngluargglutyrargaspsersergluserthrtrplyslys
202530
pheileserilethrmetalaleuleualailepheglyleuleuleu
354045
phepheglytrpglnthrvalasnglyglnservalleuproalaala
505560
glnglyproglyileilevallysalaasnargasphisaspleugln
65707580
thrasnasngluvalarglysalaserlyslysleutyrgluleuleu
859095
alaaspthrgluglyalatyrtyraspalaaspalametvalleugly
100105110
serthrvalpheserprohisthrtyrserargglnprotyrvalala
115120125
asnglytyrileglyserargileproasnvalglypheglytyrala
130135140
gluaspvaltrpasniletrpalaasnalaaspaspthrvalprogly
145150155160
alaleugluasnglytrpproleuargasnargargphealaglyala
165170175
phevalseraspphetyrcysleuglngluserleuasnserthrasn
180185190
pheprogluleuasplysasnglytyrthrthrvalileserserile
195200205
proglntrpthrglnleuglnpheserleuasnasnglulystrpphe
210215220
aspproglnthrvalaspprolysaspvalalaasntyrthrglnglu
225230235240
leusermetglnasnglyilevalthrthrglnleuasptrpleuasp
245250255
glyleuleuhisvalgluthrglnvalleualahislyslysiletyr
260265270
thrleuglyilevalsermetlysvalserleuasnvalaspargleu
275280285
proglnglygluserservalgluleuasnpheargaspvalleuasp
290295300
pheasnthrserhisargthrvalleualaasplysglytyraspglu
305310315320
glyiletyrmetvalvalgluprogluasnvalprotyrserasnthr
325330335
sermetpheserlyscysgluileaspasnaspglyvallyspheasn
340345350
glupheglyseraspaspgluthrvalserglnasnalaservalile
355360365
leuthrlysaspasnproglnvalthrilehislystyrvalglyile
370375380
pheserthrglutyrtyrasnglyprothrleuasnargalalysglu
385390395400
valalavalthrsertyrglyasntyrasplysleuthrlysalahis
405410415
asnglualatrplysasniletyraspasnalapheilegluilepro
420425430
seraspgluvalleuglumetthralaargserserleutyrhisleu
435440445
leualaasnthrargthrtyrasnvalseralaglulysglyleupro
450455460
valprovalserglyleuserseraspsertyrglyglymetvalphe
465470475480
trpaspseraspphetrpileglnproglyileleuprophephepro
485490495
aspilealalysasnileasnasntyrargasnalathrhisglugln
500505510
alaglnleuasnalalysglntyrglytyrproglyalaleutyrpro
515520525
trpthrserglyargtyralaasncysthrserthrglyprocysval
530535540
asptyrglutyrhisileasnvalaspvalcysleualaserpheser
545550555560
iletyrleuasnglyalalysglyileaspaspasptyrleuargtyr
565570575
thrthrtrpprometvalhisaspalaalagluphephethralaphe
580585590
vallystyrasnserthrileaspsertyrgluthrhisasnleuthr
595600605
aspproaspgluphealaasnhisvalaspasnglyalaphethrasn
610615620
alaglyilelysleuleuleulystrpalathraspileglytyrhis
625630635640
leulysgluprovalaspproargtrpmetgluileserglulysile
645650655
hisileproglnseraspserasnilethrleuglutyrserglymet
660665670
asnserservalgluilelysglnalaaspvalthrleumetvaltyr
675680685
proleualapheilethraspgluserileleuasnasnalailelys
690695700
aspvaltyrtyrtyrsergluhisglnserserthrglyproalamet
705710715720
thrtyrprovalphevalalaalaalaalaglyileleuasnhisgly
725730735
serserserglnsertyrleutyrlysserleuleuprotyrleuarg
740745750
alaprophealaglnphesergluglnseraspaspasnpheleuthr
755760765
asnglylysthrglnproalaphepropheleuthralaasnglygly
770775780
pheleuglnserileleupheglyleuthrglyleuargtyrsertyr
785790795800
gluvalgluproglyserlyslysileglnargileleulyspheasn
805810815
proilegluleuproleuleuproglyglyilealaileargasnphe
820825830
lystyrleuglyglnvalleuaspileileileaspaspargasngly
835840845
thrilevalhislysserglyaspglnproileargilelysvalpro
850855860
aspargthrleuilehisaspargaspilethrprotyrhisglyasn
865870875880
glyseralaglymetgluserilelysileaspasnthrthrhisgly
885890895
thrseralahistyrtyrthrileasnproglyglugluleuileleu
900905910
proleuphehismetaspglnasnvalgluglyasnleuvalgluser
915920925
lysglnilethrasnleuthralaglyvalproglyaspvalserile
930935940
serthrleuaspglyasnasntyrthrhistrpglnproalavallys
945950955960
serleuproglylysleuleuileaspleuglyalaasnasnsergln
965970975
valileasnlysglymetileleutrpglyglnargproalalysasn
980985990
phethrileserileleuprohisthraspvalileglyglnileleu
99510001005
seraspleuthrthrileleugluasnalaasnvalglyaspglu
101010151020
servalleuglualaleugluaspileglyglyserserproleu
102510301035
leuserhisthrglyaspthraspvallysaspleuleusertrp
104010451050
lysalagluaspvalaspaspileleuleuglyileserglyglu
105510601065
serasnvalileargglylysphevalproileleulysasptyr
107010751080
lysvalgluprosergluprotyrpheglugluiletyrserlys
108510901095
serileilegluileleuproserasnlysthrgluphealaile
110011051110
asptyrglulysvalglnhisasnserthrsercysserhislys
111511201125
aspproseraspasncysthrasptrpthrlysthrargpheile
113011351140
metvalserilegluglysertyraspaspaspaspaspgluarg
114511501155
glyglythrilelysgluilevalleumetasn
11601165
<210>65
<211>1278
<212>prt
<213>达氏诺莫维酵母(naumovozymadairenensis)
<400>65
metserpheleulysargilephephelysgluargproproileile
151015
thralathrgluvalglytyrileserserasncysaspvalgluthr
202530
tyrproleuthrserthrgluthrhisileaspasnasnileasnile
354045
aspthrasnasnthrgluleutyrthrasnaspserasnasnasnasn
505560
asntyrasnasnaspasnileprothrileglygluglnargarggln
65707580
leuasnlysthrpheasnlysalathrilethrlystyrleuleuala
859095
pheilemetilealaglyleuilealaleualaalavalalaserthr
100105110
ileaspleuserseralapheserlysglylysserserglnsermet
115120125
aspgluaspileasnasnileglnprogluthrproseralatyrlys
130135140
gluglyileileserserthrseraspthrasnasnprohisvalleu
145150155160
glytyraspprogluthrarglysserserlyslysleutyrgluleu
165170175
methisasppheaspthralatyrtyraspaspgluasnmetileleu
180185190
glythrasnleuphesergluasnvaltyrserargglnprotyrval
195200205
alaasnglytyrileglyserargileproasnthrglypheglytyr
210215220
alaleuaspthrleuasnleuphethraspalagluglyserleuasn
225230235240
asnglytrpproleuargasnargargtyralaglyalaphevalser
245250255
aspphetyrserleuglnglulysleuasnserthrasnpheproglu
260265270
leuaspasnhisglytyrserthrvalileserserileproglntrp
275280285
thrasnleuglnpheserleuaspasnglyseriletrppheasnpro
290295300
glngluileasplysthraspvalserasntyrglnglnasnleuser
305310315320
metlysaspglyilevalthrthraspmetasptrpileaspglymet
325330335
ilehisvallyssergluiletrpalahisarglysiletyrproleu
340345350
glymetvalserleugluileserleuasnvalaspserileproasp
355360365
glyphegluservalaspleuaspvaltrpaspvalleuasppheasn
370375380
thrserhisargthrvalleuasnaspileglytyraspaspaspgly
385390395400
iletyrmetvalilegluproaspasnvalprotyrserasnalaala
405410415
leutyrseralacysthrleuglnleugluasnserthrglugluhis
420425430
propheglnpheaspgluasplysaspproleumetasnthrasnglu
435440445
argileserlysvalserhisvalthrleuserlysalaasnprolys
450455460
leuthrvalhislystyrvalglyilemetserthrglutyrasnasn
465470475480
aspglulysasnglyglyalaserasnleuaspleualathrlysval
485490495
alametserserlysglysertyraspleuleuleuserthrhislys
500505510
lysalatrptyralaleutyrasnaspalapheilegluileproser
515520525
aspasnleuleuglumetthralaargserserleutyrglnleuleu
530535540
alaasnthrargthrtyrasnvalserglugluargglyleuproleu
545550555560
alavalglyglyleuserseraspsertyrglyglymetvalphetrp
565570575
aspseraspiletrpvalglnproalaleuleuprophepheprogln
580585590
ilealalysasnileasnasntyrargasnalathrhisalaglnala
595600605
argleuasnalalysglntyrglytyrproglyalavaltyrprotrp
610615620
thrserglyargtyralaasncysthrseralaglyprocysvalasp
625630635640
tyrglutyrhisileasnvalaspilealametalathrpheserile
645650655
tyrmetasnglyglylysaspvalaspaspasntyrleuargtyrthr
660665670
thrtrpprometvalargaspalaalagluphephethrvaltyrval
675680685
lystyrasnalathrleuglylystyrgluthrtyrasnleuthrasp
690695700
proaspgluphealaasphisileasnasnglyalaphethrasnala
705710715720
glyilelysthrleuleulystrpalathraspileglyasnhisleu
725730735
glyglutyrvalaspprolystrpmetgluileserhisasniletyr
740745750
ileproargserlysserasnilethrleuglutyrserglymetasn
755760765
serservalgluilelysglnalaaspvalthrleumetvaltyrpro
770775780
leuglytyrileasnaspgluserileleuasnasnalailelysasp
785790795800
leutyrtyrtyrsergluargglnseralaserglyproalametthr
805810815
tyrprovalphevalalaalaalaserthrleuleuasnhisglyser
820825830
serserglnsertyrleutyrlysservalleuprotyrleuargala
835840845
prophealaglnphesergluglnseraspaspasnpheserthrasn
850855860
glyleuthrglnproalaphepropheleuthralaasnglyglyphe
865870875880
leuglnserileleupheglyleuthrglyileargtyrthrtyrglu
885890895
valaspglualathrlyslysileasnargvalleuargpheasnpro
900905910
valgluleuproleuleuproglyglyilealaileargasnphelys
915920925
tyrmetaspglnvalleuaspileileleuasnasphisasnglythr
930935940
ilevalhislysserglyglylysproileasnilelysvalproasn
945950955960
arglysleuilehisaspargaspileaspleuphethrargaspglu
965970975
lysarggluserleugluargargaspglntyrglulysgluasppro
980985990
asnaspthrserasnaspalalysaspserthrlystyrglythrser
99510001005
tyrvalleuhisproglyglugluleuthrileproleuphelys
101010151020
progluleuasnilegluglyasnilealagluserlysglnile
102510301035
thrasnleuthralaglyvalproglyaspvalglypheserala
104010451050
leuaspglyasnasntyrthrhistrpglnproalaasplyslys
105510601065
aspasnalaargleuleuileaspleuglygluaspasnilelys
107010751080
gluilearglysglymetileleutrpglyglnargproalalys
108510901095
asnileserilealaileleuprohisthraspthrvalgluasn
110011051110
ilephethrasnvalserlysileleugluasnserlysasnala
111511201125
gluglutyrarggluglyvalileaspleuleuasngluasnasn
113011351140
ileservalglnserthrserserthrglygluvalglualaasn
114511501155
thraspleuglnserileilesertrplysthraspasppheasp
116011651170
gluvalleugluargpheproargleuglyileleuglygluasn
117511801185
phevalthrileleuglnasptyrlysilegluproserglupro
119011951200
tyrtyrglugluvalilelysgluserleuilegluileleupro
120512101215
serasnlysthrglupheserileasptyrserargilehisile
122012251230
asnserthrargasnasnthrgluileglyglutrplyslysala
123512401245
argtyrvalleuleuthrvalhisglythrtyraspaspaspile
125012551260
aspprolysglyalathrilelysgluilealaphemetglugln
126512701275
<210>66
<211>1152
<212>prt
<213>耐热拉茜斯酵母(lachanceathermotolerans)
<400>66
metservalarghislysthrarglyshisthralathrserleuile
151015
alaargserleuproglyleuargalailephevalileleualathr
202530
cysvalalaalaalaglytrpargtrpleuleuthrglygluleupro
354045
arglysprohisleuprocyslysvalpheargthrhisarghishis
505560
aspthrargileglutrpargargargsersergluserleutyrglu
65707580
leuleuthraspserglnaspthrtyrtyraspgluaspasntrpthr
859095
valglythrvalphephesergluasnthrtyrserargglnprotyr
100105110
valalaasnglypheleuglyserargileproasnvalglyphegly
115120125
tyralaproaspthrtyrasniletrpthrproaspproserilepro
130135140
glyalaleuasnasnglytrpproleuargasnglnargphealagly
145150155160
alaphevalseraspphetyrserleuglnprohisleuasnserthr
165170175
asnpheprogluleuaspalaaspglytyrserthrileleuserala
180185190
ileproglntrpthraspmetlysileservallysasnaspthrval
195200205
argphethralaglnaspvalleuproglnhisvalserasntyrhis
210215220
glnasnleuserleuleuthrglythrvalthrthrgluleuasptrp
225230235240
metglyleuleuhisilelysserthrvalleualahisargileleu
245250255
hisproleuglyvalleulysleugluleuserleuileproaspser
260265270
glyleulysthrleuaspleuglnvalseraspilepheasnhisser
275280285
thrserargargthrpheleuargaspleuglytyraspserarggly
290295300
iletyrmetgluvalgluproaspasnvalprotyrserseralaala
305310315320
leutyrserserleuargpheaspglyproalaglualaaspglycys
325330335
ilepheglnleuseraspmetasnthrthrvalserarglysasnphe
340345350
leuthrleusercysglulysproserilethrileglnlystyrval
355360365
alavalvalserthrglutyraspargtyrasnlysthralaserasn
370375380
metglualaalalysaspilevalhisserglncysglyglntyrasp
385390395400
leuilethrglnalahishisasnglutrpvalserleutyraspasn
405410415
alaserilegluileproseraspserleuleugluleuseralalys
420425430
serserleuphehisleuleualaasnthrargargpheasnileser
435440445
serserargglyleuproleuproserserglyleuserseraspser
450455460
tyrglyglymetilephetrpaspalaaspiletrpileleuproala
465470475480
leuleuprophepheproaspilealaarghismetvalasptyrarg
485490495
lysasnthrhisgluglnalaargleuasnalaglnglntyrglytyr
500505510
proglyalaleutyrprotrpthrserglyargtyralaasncysthr
515520525
serthrglyprocysvalasntyrglutyrhisileasnileaspile
530535540
alametalaserpheleuvaltyrleuasnglyalaproglyvalasp
545550555560
aspglutyrleuarggluthrthrtrpproleuvalargaspalaala
565570575
glnphephethralatyrvallyspheasnserserleuaspalatyr
580585590
gluthrhisasnmetthraspproaspglutyralaasnpheileasn
595600605
asnglyalaphethrasnalaglyilelysthrleuleulystrpala
610615620
thraspvalglyasnhisleuglnglugluileaspproargtrpval
625630635640
gluvalserasplysmetpheileproilealagluserasnilethr
645650655
leuglutyrserglymetasnalaservalgluilelysglnalaasp
660665670
valmetleumetthrtyrproleuglytyrilethraspgluthrile
675680685
leuaspasnalaileargasnleutyrtyrtyrsergluargglnser
690695700
proserglyproalametthrtyrprovalphevalalaalaalaala
705710715720
glyleuleuasnhisglycysserserglnsertyrleutyrlysser
725730735
valleuprotyrleuargalaprophealaglnphesergluglnser
740745750
aspaspasnpheleuthrasnglyphethrglnproalapheprophe
755760765
leuthrglyasnglyglytyrleuglnserleuleupheglyleuthr
770775780
glyleuargtyrsertyrgluvalasplysglnthrlyslysilethr
785790795800
argleuleulyspheasnproilelysleuproleuleuglnglygly
805810815
ilealaileasnasnphelystyrmetglyglnvalleuaspileile
820825830
ileglyaspglulysalathrilevalhislysileglyaspglupro
835840845
ileasnleulysleuileaspargserpheilehisaspargaspval
850855860
thrglyprohismetthrhisvalhisgluglyglugluvalproval
865870875880
glntrpilethrleuglnproglyglnasnleuthrvalaspleuphe
885890895
argproleuleuasnileaspglyasnleuilegluglylysglnval
900905910
serasnphethralaglyvalproglyaspvaliletyrservalile
915920925
aspglyasnasnphethrhistrpglnproleuasplysasnvalcys
930935940
alalysileleuileaspleuglyproglyasngluglnasnleuthr
945950955960
glyglymetileleutrpglyargargproalaargasnileserval
965970975
alametvalprotyrlysglyaspilegluglnalaleuglnlysala
980985990
serservalmetgluserserglyproasplysargvalgluileleu
99510001005
glytyrthralaproalasergluleualaserargleuserglu
101010151020
thrgluaspargphepheglugluasptyrargleuleuleuser
102510301035
serglnargleuaspilegluglnleuserlysleuserproglu
104010451050
serserleuleulysserlysphevalthrileleuhisasnleu
105510601065
glnileserprosergluprotyralaglugluasnglnarggly
107010751080
serlysilevalileproargserasnserthrhisphethrphe
108510901095
asptyrserasntyrthrserasplysalaphegluhisproval
110011051110
serlysgluserglyalasertrpproproproargphevalleu
111511201125
valcysileglnglythrtyraspaspaspaspaspprolysgly
113011351140
alathrilelysgluilealaleumet
11451150
<210>67
<211>1152
<212>prt
<213>魁北克拉茜斯酵母(lachanceaquebecensis)
<400>67
metservalarghislysthrarglyshisthralathrasnleuile
151015
valglyserleuproglyleuargalailephevalileleuthrala
202530
cysvalalaalavalglytrpargtrpleuleuthrglylysleupro
354045
lyslysprohisleuprocyslysvalpheargthrhisarghishis
505560
aspthrargileglutrpargargargsersergluserleutyrglu
65707580
leuleuthraspserglnaspthrtyrtyraspgluaspasntrpile
859095
valglythrvalphephesergluasnthrtyrserargglnprotyr
100105110
valalaasnglypheleuglyserargileproasnileglyphegly
115120125
tyralaproaspalatyrasniletrpalaalaaspproserilepro
130135140
glyalaleuasnasnglytrpproleuargasnargargtyralagly
145150155160
alaphevalseraspphetyrserleuglnprohisleuasnserthr
165170175
asnpheprogluleuaspalaasnglytyrserthrvalleuserala
180185190
ileproglntrpthraspmetlysleuserilelysasnaspthrval
195200205
argphethralaglnaspvalleuproglnhisvalthrasntyrhis
210215220
glnasnleuserleuleuthrglythrvalthrthrgluleuasptrp
225230235240
metglyileleuhisilelysserthrvalleualahisargthrleu
245250255
hisproleuglyvalleuasnmetgluleuserleuleuserasnser
260265270
glyleulysthrleuaspleuglnvalseraspilepheasnhisser
275280285
thrserargargthrpheleuargaspleuglytyraspserglngly
290295300
iletyrmetgluvalgluproaspasnvalprotyrserseralaala
305310315320
mettyrserserleuargpheileglyserglygluseraspglycys
325330335
ilepheglnleuseraspmetasnthrthrvalserarglysasnphe
340345350
leuthrleuserasnglulysproserilethrileglnlystyrval
355360365
alavalvalserthrglutyraspargtyrasnlysthralaserasn
370375380
metgluleualalysaspilevalargserglncysglyhistyrasp
385390395400
serilethrglnmethishisaspglutrpvalserleutyrglyasn
405410415
alaserilegluileproseraspserleuleugluleuseralaarg
420425430
serserleuphehisleuleualaasnthrargargpheasnileser
435440445
serserargglyleuproleuproserserglyleuserseraspser
450455460
tyrglyglymetilephetrpaspalaaspleutrpileleuproala
465470475480
leuleuprophepheproaspilealaarghismetvalasptyrarg
485490495
argasnthrhisgluglnalaargleuasnalaglnglntyrglytyr
500505510
proglyalaleutyrprotrpthrserglyargtyralaasncysthr
515520525
serthrglyprocysvalasptyrglutyrhisileasnileaspile
530535540
alametalaserpheleuvaltyrleuasnglyalaproglyvalasp
545550555560
aspglutyrleuarggluthrthrtrpproleuvalargaspalaala
565570575
leuphephethralatyrvallystyrasnserserleuaspalatyr
580585590
gluthrhisasnmetthraspproaspglutyralaasnpheileasn
595600605
asnglyalaphethrasnalaglyilelysthrleuleulystrpala
610615620
thraspvalglyasnhisleuglnglugluvalaspproargtrpval
625630635640
gluvalserasnlysmetpheileproilealagluserasnilethr
645650655
leuglutyrserglymetasnalaservalgluilelysglnalaasp
660665670
valvalleumetthrtyrproleuglytyrilethraspgluthrile
675680685
leuaspasnalaileargasnleutyrtyrtyrsergluargglnser
690695700
alaserglyproalametthrtyrprovalphevalalaalaalaala
705710715720
glyleuleuasnhisglycysserserglnsertyrleutyrlysser
725730735
valleuprotyrleuargalaprophealaglnphesergluglnser
740745750
aspaspasnpheleuthrasnglyleuthrglnproalapheprophe
755760765
leuthrglyasnglyglytyrleuglnserleuleupheglyleuthr
770775780
glyleuargtyrsertyrgluvalaspglnlysthrlyslysilethr
785790795800
argleuleulyspheasnproilelysleuproleuleuglnglygly
805810815
ilevalileasnasnphelystyrmetglyglnvalleuaspileile
820825830
valglyaspgluasnalathrilealahislysvalglyaspglnpro
835840845
ileasnleulysleuileaspargasnpheilehisaspargaspval
850855860
alaileseraspilelyshisvalhisgluserglulysvalproval
865870875880
glnargvalthrleuglnproglyglnasnleuthrvalaspleuphe
885890895
hisproleuthrasnileaspglyasnleuvalgluglythrglnval
900905910
serasnphethralaglyvalproglyaspvaliletyrservalile
915920925
aspglyasnasntyrthrhistrpglnproleuasnlysasnalacys
930935940
alalysileleuileaspleuglyproglyasngluglnasnleuthr
945950955960
glyglymetileleutrpglyargargproalaargasnileserile
965970975
alametvalprotyrlysglyaspilegluglnalaleuglulysala
980985990
serlysvalilegluserasnglyproglylysargvalgluileleu
99510001005
glytyrthralaproalaservalleualaserargleuserglu
101010151020
alalysasptrphispheglugluasptyrargleuleuleuser
102510301035
serglnargleuaspilegluglnleuserlysleuserproasp
104010451050
serserleuleuaspserlysphevalthrileleuasnasnleu
105510601065
glnileserprosergluprotyralaglugluasnglnarggly
107010751080
serlysilevalileproargserasnserthrhisphethrphe
108510901095
asptyrsersertyrthrasnasnthrthrpheasphisleuthr
110011051110
serasnaspserglyalasertrpproproproargphevalleu
111511201125
valcysileglnglythrtyraspaspaspaspaspproarggly
113011351140
alathrilelysgluilealaleumet
11451150
<210>68
<211>1202
<212>prt
<213>毕赤四盾酵母(tetrapisisporaphaffii)
<400>68
metphepheaspasnvaltyrserglnarglysglulysaspleuval
151015
leuleuiletrppheglnargilepheileilemetleuleuileile
202530
pheleuilepheasncyspheilethrvalglnserilehisileasn
354045
serphepheasnasnlyspheleuarghispheserthrargasnasn
505560
lysleuiletyralaserproilealaglnserasnleuserileleu
65707580
servalglyglugluileserglnvalalathralaarglysthrser
859095
lyslysleutyraspleuleuileasntyrgluaspsertyrtyrasp
100105110
aspgluasnmetvalleuglythraspvalilethrlysasnthrtyr
115120125
thrargglnprotyrvalalaasnglytyrileglyserargilepro
130135140
asnileglypheglytyralaleuaspglutyrseriletrpasnpro
145150155160
aspvalthraspasnprogluleupheasnasnglytrpproleuarg
165170175
asnargargtyralaglyserphevalseraspphetyrserleugln
180185190
lyslysleuasnserthrasnpheprogluileaspaspileglytyr
195200205
serasnvalileserserileproglutrpthrasnilelysleuthr
210215220
valileaspgluasnaspargserpheasnpheasnproglnaspile
225230235240
servalasnaspilethrasntyrhisglnasnleusermetglnasn
245250255
glymetvalthrthraspleuasntrpleuasnasnthrleuhisile
260265270
asnserthrmetphethrhisarglysleutyrproleuglyleuleu
275280285
gluileaspilethrleuasnargglutyrleuprothrasppheala
290295300
thrtyrasnileservalpheaspileleuasppheasnthrserhis
305310315320
argthrpheleuargaspmetglyprosertyraspserserlysmet
325330335
glnglyilephemetvalvalgluprogluasnvalprotyrthrasn
340345350
cysvalilepheserthrthrgluilegluglnleuaspilethrgly
355360365
servalhisargasnilethrleuaspasptyrvalileaspglymet
370375380
asnserserileserglnvalalalysaspilethrleuserthrglu
385390395400
pheprolysleulysiletyrlystyrserserileileserserasn
405410415
tyraspglycyslysasnasnthrtyrglnaspasnleuasngluala
420425430
lyshisleualaleuasnserargglyasnpheglnasnleualaasp
435440445
serasnthrglnalatrpvalaspleutyraspasnalapheileglu
450455460
ileproseraspservalleugluleualaalaargserserleuphe
465470475480
hisleumetserasnalaargleuhisasnilesergluasparggly
485490495
leuproleuprovalserglyleuserseraspsertyrglyglyleu
500505510
valphetrpaspalaaspvaltrpmetglnproalaileleuprophe
515520525
pheproaspvalalalysasnileasnasntyrargasnalathrhis
530535540
lysglnalaglnleuasnalalysglntyrglytyrasnglyalatrp
545550555560
tyrprotrpthrserglyglypheserasncysthrserthrglypro
565570575
cysileasptyrglutyrhisileasnvalaspilealaleualathr
580585590
pheseriletyrmetasnglyalaaspglyileaspaspthrtyrleu
595600605
lystyrthrvaltrpproilevalargaspalaalailealaphethr
610615620
glutyrvalglntyrasplysglnleuaspvaltyrgluthrpheasn
625630635640
leuthraspproaspgluphealaasnhisvalasnasnglyalaphe
645650655
thrasnalaglyilelysileleumetlystrpalavalaspilegly
660665670
asnhisleugluglulysvalaspproiletrplysalaileasnglu
675680685
lysilevalileprolysthrserserasnilethrleuglutyrser
690695700
glymetasnserthralagluilelysglnalaaspvalproleuleu
705710715720
valtyrproleuglytyrilethraspgluserileleuasnasnala
725730735
ileargaspleutyrtyrtyrsergluhisglnalahisthrglypro
740745750
alametthrtyrprovalphevalalaalaalaalaglyleuleuasn
755760765
hisglyserserserglnsertyrleutyrlysserileleuprotyr
770775780
leuargglyprophealaglnphesergluglnseraspaspasnphe
785790795800
leugluasnglyglyleuhisproalatyrpropheleuthralaasn
805810815
glyglypheleuglnserileleupheglyleuthrglyileargtyr
820825830
sertyrlysvalaspseraspthrasnlysilealaargleuleulys
835840845
pheasnproilelysleulysleuleuproglyglyleulysilearg
850855860
asnphelystyrmetglyglnvalleuaspileileileaspasptyr
865870875880
asnglythrileilehislysserglyasnlysthrilethrilegln
885890895
ileproaspargserserileargaspglnaspileasnprotyrarg
900905910
glylysasnlysthrlysgluaspasnargileleuserlysarglys
915920925
serasniletyrgluleulysproasngluasnphethrileproleu
930935940
phevalthrgluleuasnalaproglyasnilevalgluserlysgln
945950955960
ilethrasnleuthrgluglyvalalaglyaspvalalametserala
965970975
valaspglyasnasntyrthrhistrpglnprovalasnlysalaglu
980985990
thralaargleuleuileaspleuglyproglyasngluglnthrile
99510001005
thrglyglymetpheleutrpglyglnargproalalysasnval
101010151020
serileservalleuprohisserglnalathrgluglnleuphe
102510301035
glnasnilethrtyrphealagluhispheglupheproasnarg
104010451050
gluargthrileleuglnleuileglngluvalaspasnilethr
105510601065
gluprogluvalcysglnilepheserseraspglyvalcysasp
107010751080
alaglyphethrglnleuleuasntrplysserlysaspileasp
108510901095
gluleuileglnglnpheproasnthrproglyilearggluasn
110011051110
pheilethrilevalaspasnleuservalleuproserglupro
111511201125
tyrtyrproglutyrtyrgluglnalaleuilegluileleupro
113011351140
serasnlysthrlyspheservalasptyrserlysvalglutyr
114511501155
tyrlyslyssergluvalphegluglnserglntrpprolysthr
116011651170
argpheileilevalserileglnaspthrtyrasplysaspleu
117511801185
aspprolysglyserthrilelysgluileileleutyrser
119011951200
<210>69
<211>1156
<212>prt
<213>发酵拉茜斯酵母(lachanceafermentati)
<400>69
metvalglyserileasnasnargthrcyspheserprotyrserarg
151015
servalleutyrsergluasnprosermetlysglyargpheglnala
202530
serargargphethrtyrleuglypheleuthrthrleualavalleu
354045
sertrpleuthrleualaaspvalhisasnaspaspgluvalthrglu
505560
seralaleuleuseraspargleulyspheargaspleuserserglu
65707580
asnilegluasnserarglysalaserglngluleuglulysglnile
859095
valaspsergluaspthrtyrtyraspgluglnargargileleugly
100105110
thrthrhisphesergluasnthrtyrserargglnprotyrvalala
115120125
asnglytyrileglyserargileproasnvalglypheglytyrala
130135140
leuaspthrleuasnleutrpthrproasnserthrvalalaglyala
145150155160
leuaspasnglytrpproleuargasnglnargphealaglyalaphe
165170175
valalaaspphetyrserleuglnalahisleuasnserthrasnphe
180185190
progluleuasplyslysglytyrthrthrvalilethralailepro
195200205
glntrpthraspleuserpheserleuasnaspthrphelyspheasn
210215220
provalaspvalhisseraspserilethrasntyrlysglnasnleu
225230235240
sermetsergluglyilevalthrthrglumetasptrphisaspleu
245250255
leuhisilegluthrthrilephealahisargthrilehisproleu
260265270
glyvalvalsermetaspilethrleuasnthrseralaasnleuasp
275280285
glyalaasptyrleuaspleuglnilethrasplysleuasntyrser
290295300
thrserargargalapheleuargasnleuglytyrgluthrglyser
305310315320
iletyrmetthrvalgluproaspasnvalprotyrserseralaval
325330335
leutyrserserleuglupheproserglnserleuleuasnglythr
340345350
leutyrargileasnaspasnasnserthrvalserglnasnglntyr
355360365
valargilethrlysaspserproglnileasnilelyslystyrval
370375380
glyilevalserserglupheproargaspaspasnserthrasnphe
385390395400
gluleuserlysglnilevalglugluasnileglyhistyraspser
405410415
leuleualaserhisileglnglytrpseraspleutyrasnasnthr
420425430
gluvalgluileproseraspserleuleugluleualaalaargser
435440445
serilephehisleuleualaasnthrargsergluaspvalserpro
450455460
gluargglyleuproleuprovalalaglyleuserseraspsertyr
465470475480
glyglymetvalphetrpaspalaaspiletrpmetmetproserleu
485490495
leuprophepheprogluilealalysglnilevalasntyrargasn
500505510
valthrhisserglnalavalleuasnalaglngluasnasptyrleu
515520525
glyalavaltyrprotrpthrserglyargtyralaasncysthrser
530535540
thrglyprocysileasptyrglutyrhisileasnvalaspileala
545550555560
metserserpheleuleutyrleuserglyglyalaglualaaspglu
565570575
asptyrleuargtyrthrthrtrpproileleuargaspalaalaarg
580585590
phephethrsertyrvallyspheasnserthrleuglymettyrglu
595600605
thrlysasnmetthraspproaspgluphealaasnphevalaspasn
610615620
glyalaphethrasnalaglyilelysthrleuleulystrpalathr
625630635640
aspvalglythrtyrleulystyraspvalaspserlystrpalaglu
645650655
valsergluhismetpheileproileserglnalaasnilethrleu
660665670
glutyrthrglymetasnalaservalgluilelysglnalaaspval
675680685
valleumetthrtyrproleuglypheilethraspalathrileleu
690695700
asnasnalailelysaspleutyrtyrtyrsergluargglnserala
705710715720
serglyproalametthrtyrproilephevalalaalaalaserser
725730735
leuleuasnhisglycysserserglnsertyrleutyrlysserval
740745750
leuprotyrleuargserprophealaglnphesergluglnserasp
755760765
aspasnpheleuthrasnglyleuthrglnproalaphepropheleu
770775780
thralaasnglyglypheleuglnalailetyrpheglyleuthrgly
785790795800
leuargtyrsertyrlysleuaspasnserthrglygluleuvalarg
805810815
pheleulyspheaspprovalglnmetproleuleuproglyglyile
820825830
thrleuargglypheargtyrmetaspglnileleuaspilevalile
835840845
aspaspalaalaalathrvaltyrhislysserglyasnserserval
850855860
ileilegluileseraspargserileiletyraspglnserasnile
865870875880
phephegluasnglyserthrleuglnphelyslysargaspleupro
885890895
glythrsertyrthrleugluproglyserglnleuvalpheproleu
900905910
phelysproasnleuasnileaspglyasnilealagluglylysgln
915920925
ilethrasnphethralaglyvalserglyaspvalvaltyrserval
930935940
ileaspglyasnasntyrthrhistrpglnproleuasplystyrglu
945950955960
asnalalysleuleuileaspleuglyproglyasnaspglualaval
965970975
thrhisglymetileleutrpglyglnargproalaargasnmetser
980985990
iletyralaleuleuleusergluglnthrgluleuleuleuglnasn
99510001005
thrserserthraspglntyrgluileglnaspilemetserpro
101010151020
serleuasnasngluphelysasnaspileaspgluasppheglu
102510301035
lyspheleuthrtrplysmetleuaspileasnaspphealagln
104010451050
ilealaserasnleuthrileleugluglyargphevalproleu
105510601065
leugluasnleuargilethrprosergluprotyrtyrglugln
107010751080
hisglulysasnsergluileaspileleuproserasnargthr
108510901095
lysphethrileasntyrglnasnvalseralatyrglyglyala
110011051110
asnserserasnaspasnserserseralapheileproargtyr
111511201125
tyrleuileservalgluglythrtyraspaspaspaspaspasp
113011351140
asnglyalathrileasngluilealapheilealaasn
114511501155
<210>70
<211>1134
<212>prt
<213>假山毛榉拉茜斯酵母(lachanceanothofagi)
<400>70
metglulyshislysilelysglnserarglysglycysmetmetgly
151015
ilepheleuthrleualavalleucyssertrpvalleuvalargglu
202530
leuprolysvalalaargserprocyslysvalpheargserlyslys
354045
leuphehisaspalaargilegluargargargargthrsergluthr
505560
leutyrgluleuleuseraspserargaspthrtyrtyraspgluglu
65707580
alatrpvalvalglythrserphephesergluasnthrtyrserarg
859095
glnprotyrvalalaasnglytyrleuglyserargileproasnval
100105110
glypheglytyralaleuaspthrtyrasniletrpthrproaspala
115120125
serileproglyserleuaspasnglytrpproleuargasnargarg
130135140
phealaglyalaphevalseraspphetyrglyleugluprolysphe
145150155160
asnserthrasnpheprogluleuaspglulysglytyrasnserval
165170175
leucysalaileproglntrpthraspilelyspheserilelysasn
180185190
aspthrpheglnpheasnalaglnservalasnproserglnileser
195200205
asntyrglnglnasnleuserleuleuthrglythrvalthrthrglu
210215220
metasptrpleuaspvalleuhisilelysserservalphealahis
225230235240
argilearghisproleuglythrvalglnleugluileserleuile
245250255
proasnasnprogluglnleugluvalileaspleuglnleuserasp
260265270
valleuasnhisserthrserargargthrpheleuargaspilegly
275280285
tyraspserglyglyiletyrmetgluvalgluproasnasnvalpro
290295300
tyrserseralaalaleutyrserserleulysileserseralaglu
305310315320
thrasnileaspglyvalvalpheglnleuasnaspglyglythrser
325330335
valthrcyslyshisleuleuglnleuthrseraspasnserthrval
340345350
thrileasnlysphevalglyilevalserserglutyraspargtyr
355360365
asnlysalametserasnleuaspalaalalysaspvalvalargtyr
370375380
thrglnthrglusertyrgluserleualagluglyhisglnglnglu
385390395400
trpilelysleutyraspasnalaserilegluileproseraspser
405410415
leuleugluleualaalaargserserleuphehisleuleualaasn
420425430
thrargargpheasnvalserglngluargglyleuprovalproala
435440445
serglyleuserseraspsertyralaglyleuvalphetrpaspala
450455460
aspleutrpilemetproserleuleuprophepheproaspileser
465470475480
lyshismetvalthrtyrargglnserthrhisasnglnalalysile
485490495
asnalaglngluasnglytyrproglyalaleutyrprotrpthrser
500505510
glyargphealaasncysthrserthrglyprocysvalasptyrglu
515520525
tyrhisileasnileaspvalalametalaserpheleuvaltyrleu
530535540
asnglyalaaspglyileaspaspasntyrleuargglythrthrtrp
545550555560
proleuvalargaspalaalaleuphephesersertyrvalasptyr
565570575
asnproalaleuaspalatyrgluthrhisasnmetthraspproasp
580585590
glutyralaasnpheileserasnglyalaphethrasnalaglyile
595600605
argthrleuleulystrpalathraspileglyhishisleuasnglu
610615620
servalaspprolystrpalaaspvalserlyslysilevalilepro
625630635640
ileseraspthrasnilethrleuglutyrserglymetasnalaser
645650655
valgluilelysglnalaaspvalmetleumetthrtyrproleugly
660665670
tyrilethraspgluthrileleuaspasnalaileargasnleutyr
675680685
tyrtyrsergluargglnseralaserglyproalametthrtyrpro
690695700
valphevalalaalaalaalaglyleuleuasnhisglycysserser
705710715720
glnsertyrleutyrlysservalleuprotyrleuargalaprophe
725730735
valglnphesergluglnseraspaspasnpheleuthrasnglyleu
740745750
thrglnproalaphepropheleuthrglyasnglyglytyrleugln
755760765
serleuleupheglyleuthrglyleuargtyrsertyrgluserasn
770775780
lysthrthrglyglnilethrargleuleulyspheasnproilelys
785790795800
leuproleuleualaglyglyilethrileargasnphelystyrmet
805810815
glyglnvalleuaspvalgluileglyaspgluasnalathrleuval
820825830
hislysalaglyaspglnproileargleulysleuleuasnargasn
835840845
valilehisasparggluleuglyargpheaspserserhisvalhis
850855860
gluleulysglnleuprovalarghisalaileleugluproglyarg
865870875880
asnleuthrvallysleupheargproleuleuasnileaspasnasn
885890895
leualagluglylysglnvalthrasnphethrthrglyvalprogly
900905910
aspvaliletyrservalthraspglyasnasntyrthrhistrpgln
915920925
proleuhislyscysaspcysalalysileleuileaspleuglyala
930935940
aspasngluglnthrleuthrglyglymetilemettrpglylysarg
945950955960
proalaglnasnileservalsermetpheprophehisglyserile
965970975
gluglualaleupheasnalaseralaileileglumetserglypro
980985990
gluthrgluthrgluilemetglyphelysglyalaalaseraspval
99510001005
lysleulysleulysgluthrlysgluasncyspheglugluasp
101010151020
tyrglntyrleumetglnserglnargleuaspileglyglnleu
102510301035
serlysileserprogluserserleuleulysserlyspheval
104010451050
thrileleuargaspglnlysvalserproservalprotyrile
105510601065
gluasnaspgluasnlysaspargilemetileproglnserasn
107010751080
thrthrglnphethrpheasptyrsertyrtyrserasnhisser
108510901095
cysalaalaasptrpproproproargphevalleuvalhisval
110011051110
glnglythrtyraspasnaspaspaspproargglyalathrile
111511201125
lysgluilealaleumet
1130
<210>71
<211>1066
<212>prt
<213>蟑螂四盾酵母(tetrapisisporablattae)
<400>71
metilepheasnileleupheserilephepheasnilepheileile
151015
leuglntyrleuglnvalvalserglyleulysserglnasnlysthr
202530
asnasnleutyrglnileleuileaspasnasnaspasnsertyrtyr
354045
aspserthrasnglnileleuglythrlysglnphethrlysglnhis
505560
alatyrserargglnprotyrvalalaasnglytyrileglyserarg
65707580
ileproasnvalglypheglytyralaleuaspthrileasnleutrp
859095
alaalaasnserservalgluglyalaleuasnasnglytrpproleu
100105110
argasnhisargtyralaglyserphevalseraspphephesergln
115120125
glnglulysleuthrserthrasnpheprogluleuaspgluvalgly
130135140
tyrserthrvalilealaserileproglutrpthrasnilelysile
145150155160
ileleuservalasnglythrasphisleuileserproglualaleu
165170175
ileaspgluasnasnmetserasntyrpheglnasnmetsermetser
180185190
asnglyvalvalthrthrserpheasntyrleuasnaspleuvalasn
195200205
ilelysthrgluvalphealahisarggluilesertyrleuglyile
210215220
leuserleuasnvalthrleuleuasnproasnglutyrmetmetasp
225230235240
glnileaspileglnvalvalaspileleuasppheasnserserhis
245250255
argthrvalleuasnglutyrglytyraspleulysasnglyalaile
260265270
phemetservalserprogluasnthraspaspservalalaserleu
275280285
tyrserthrleuglnilegluasnaspileglutyrasnthralatyr
290295300
serlysileasnglulysilegluhisserleusercyslysileasp
305310315320
leuasnasnprothrileilepheasnlysphevalglyileileser
325330335
thrglutyrglyasnaspalaasppheasnserthrphelysmetala
340345350
lysserserileleuaspleuserleuasntyrthrglnleutyrasp
355360365
serasntyrvalalatrplyslysleuleugluasnthraspleuser
370375380
ileproglyaspserleuleuaspmetthralalysserserilephe
385390395400
hisleuleuserasnthrargserlysasnvalalaaspasnarggly
405410415
leuprovalservalserglyleuserseraspsertyrglyglymet
420425430
valphetrpaspseraspleutrpileglnproserleuleuprophe
435440445
pheprolysileserasnlysmetileasntyrargasnalathrhis
450455460
gluglnalalysileasnalaasnsertyrglytyraspglyalaleu
465470475480
tyrprotrpthrserglylysphealaasncysthrserthrglypro
485490495
cyspheasptyrglutyrhisileasnileaspilealaleualaala
500505510
ileserleutyrmetserglyglyglyaspgluasptyrleuargtyr
515520525
thralatrpproilevalasnaspalaalalysphepheserglutyr
530535540
valglntyrasngluthrserglymettyrvalthrglnasnmetthr
545550555560
aspproaspgluphealaasppheileaspasnglyalaphethrasn
565570575
alaglyileargserleuleulystrpalathraspileglyhishis
580585590
leuasngluthrileaspgluasntrpmetgluileserlysasnleu
595600605
tyrileprolysalaaspthrasnilethrleuglutyrthrglymet
610615620
asnserthrthrlysvallysglnalaaspvalalaleumetvaltyr
625630635640
proleuglypheilethraspgluserasnleuasnaspalavallys
645650655
aspleuphetyrtyrsergluhisglnseralaalaglyproalamet
660665670
thrpheprovalphevalalaalaalaserserleuleuasnhisgly
675680685
thrserserglusertyrleutyrlysservalilepropheleuarg
690695700
glyprophealaglnphesergluglnalaaspaspaspphemetlys
705710715720
asnglygluthrglnproalaphepropheleuthralahisglygly
725730735
pheleuglnserileleupheglyleuthrglyileargtyrsertyr
740745750
glumetasnthrglnthrasnlysilegluargpheleuargpheasp
755760765
proilelysleugluleuleuasnglyglyleuhisileglnglyphe
770775780
hispheleuglyglnthrleuaspileserthraspaspaspphegly
785790795800
tyrilelyshislysasnglythrglnasnileargilelysvalpro
805810815
thrargasntyrpheseraspgluvalaspilephethrargasnasn
820825830
lysthrserasnpheglulysargthrileglyasngluphephegly
835840845
argglugluglulysserlyssertyrserasnglythrpheilethr
850855860
leugluproglyglugluvallysileproleuphelysproleulys
865870875880
asnileproserasnleualagluglylysglnvalalaasnleuthr
885890895
gluglyvalproglyaspvalpropheserleuvalaspglyasnasn
900905910
tyrthrhistrpglnproalaasnlysserserileserargvalile
915920925
ileaspleuglyalatyrasnserserasnileserglyglyglnile
930935940
leutrpglyserargproalalystyrpheserileservalilepro
945950955960
servalpheasnphethrthrlysvalglnmetasnlysileileglu
965970975
glugluserileservalileaspasnglnvalvalleuproserlys
980985990
prophetyralagluilevalgluaspserileileaspleuleupro
99510001005
serasnvalthrasnpheglupheasnphelysasnileasnglu
101010151020
lystyrproasnhisserasnileserasnleuileargtyrval
102510301035
leupheserphegluglythrtyraspasnaspthrgluvalthr
104010451050
glyalathrvallysgluileserileileserserasp
105510601065
<210>72
<211>697
<212>prt
<213>小麦顶囊壳(gaeumannomycestritici)
<400>72
metproalametalaproleutrplysleualathralaalaalaala
151015
serserleualavalasnalaleutyrileasnglysergluvalthr
202530
procysaspserproiletyrcyshisglygluleuleulysglnval
354045
gluleualahisprophealaaspserlysthrphevalaspmetpro
505560
thrilelysprovalasngluvalleualaalapheasplysleugln
65707580
lysproleuserasnasnthrgluleuglnasnpheleuarggluasn
859095
phealalysalaglyglygluleuglugluvalproasnsergluleu
100105110
alathraspprovalpheleuasnlysleuasnaspthrvalilearg
115120125
gluphevalglulysvalileglyiletrpprogluleuthrargarg
130135140
tyrlysglyproglyglncysserglucysalaasnserpheilepro
145150155160
ileasnargthrphevalvalalaglyglyargphearggluprotyr
165170175
tyrtrpaspsertyrtrpilevalgluglyleuleuargthrglygly
180185190
alaphethrglnileserlysasnthrilegluasnpheleuaspleu
195200205
valgluthrileglyphevalproasnglyalaargiletyrtyrarg
210215220
asnargserglnleuprovalleuserglnmetvalargvaltyrval
225230235240
gluhisthrasnaspthrserileleugluargalavalproleuleu
245250255
ilelysgluhisglnphephevalasnasnargservalgluvalthr
260265270
serargserglyglnthrtyrthrleuasnargtyralavalvalasn
275280285
thrglnproargprogluserphearggluasptyrilethralaasn
290295300
asnargsertyrtyralaalaserglyileiletyrproglualaarg
305310315320
glnleuasngluserglulysalagluleutyrserhisleualaser
325330335
glyalagluserglytrpasptyrserthrargtrpleualalyspro
340345350
asnaspalaalalysaspvaltyrpheproleuargserleuasnthr
355360365
asnasnmetvalprovalaspleuasnserileleutyralaasnglu
370375380
leualaileserglupheleuasnglnthrglyasnalathrglyala
385390395400
alaglutrplysargthralagluargargserglualametpheala
405410415
leumettrpasngluthrleutrpsertyrpheasptyrasnmetthr
420425430
serlysgluglnasniletyrileproleuaspaspaspalathrala
435440445
phegluglnthrthralaproalaglylysglnvalleuphehisval
450455460
alaglnphetyrprophetrpthrglyalaalaproalaglnleuarg
465470475480
aspasnproleualavalglnargalatyrserargvalaspalatyr
485490495
leuglyleulysglyglyalaileproalathrasnleuargthrgly
500505510
glnglntrpaspgluproasnvaltrpproproleumethisileleu
515520525
metgluglyleuthrargvalproalathrpheglygluserasppro
530535540
alatrpleuglyvalglnaspleualaleuargleuglyglnargtyr
545550555560
leuaspserthrphecysthrtrptyralathrglyglyserthrser
565570575
glnthrproglnleuglnglyleuthrprogluaspthrglyilemet
580585590
pheglulystyrglyaspasnserthrasnvalalaglyalaglygly
595600605
glutyrgluvalvalgluglypheglytrpthrasnglyvalleuile
610615620
trpvalalaaspglnpheserthrargleulysargproasncysgly
625630635640
asnilethralaalaasnvalhisproserglyglyargarglysarg
645650655
serleugluglnargseralaleuglumethisserseraspalaala
660665670
trpilelysargpheglyarglysalaalalysargserleuglyarg
675680685
thralaglyleuglyglyglyalaval
690695
<210>73
<211>646
<212>prt
<213>夏季斑枯病菌(magnaporthiopsispoae)
<400>73
metalaprosertrplysleualaalaalavalthralatrpserleu
151015
alavalasnalaleutyrileasnglythrgluilethrprocysasp
202530
serproiletyrcysargglygluleuleulysglnvalgluleuala
354045
argprophealaaspserlysthrphevalaspmetprothrilelys
505560
provalaspgluvalilealaalapheasnlysleuglnlysproleu
65707580
serasnasnthrgluleuglnasnpheleuarggluasnphealagln
859095
alaglyglygluleuglugluvalproasnsergluleuthrthrasn
100105110
provalpheleulyslysleuasnaspthrvalileargglupheval
115120125
glulysvalileaspiletrpprogluleuthrargargtyrlysgly
130135140
proglyglncysseraspcysalaasnserpheileprovalasnarg
145150155160
thrphevalvalalaglyglyargphearggluprotyrtyrtrpasp
165170175
sertyrtrpilevalgluglyleuleuargthrglyglyalaphethr
180185190
glnileserlysasnthrilegluasnpheleuaspleuvalgluthr
195200205
ileglyphevalproasnglyalaargiletyrtyrargasnargser
210215220
glnproproleuleuserglnmetvalargiletyrilegluhisthr
225230235240
asnaspthrserileleugluargalavalproleuleuilelysglu
245250255
hisgluphephevallysasnargserilegluvalthrserargser
260265270
glyglnlystyrthrleuasnargtyrservalvalasnthrglnpro
275280285
argprogluserphearggluasptyrilethralaasnasnargser
290295300
tyrtyralaalaserglyileiletyrprogluglyargglnleuasn
305310315320
gluserglulysalagluleutyrsertyrleualaserglyalaglu
325330335
serglytrpasptyrcysalaargtrpleuarglysproaspaspala
340345350
alalysaspvaltyrpheproleuargserleuasnthrasnasnmet
355360365
valprovalaspleuasnserileleutyralaasngluleuthrile
370375380
serglnpheleuasnargthrglyasnalathrglyalaalaglutrp
385390395400
lysglyleualagluargargserglualametphealaleumettrp
405410415
asngluthrleutrpsertyrpheasptyrasnthrthrserglyglu
420425430
glnasniletyrvalproleuaspaspaspalathralaleuglugln
435440445
ilethralaproalaglylysglnvalleuphehisvalalaglnphe
450455460
tyrprophetrpthrglyalaalaprolysglnleuargaspasnpro
465470475480
leualavalleuglnalatyrserargvalaspalatyrleulysleu
485490495
lysglyglyalaileproalathrasnleuargthrglyglnglntrp
500505510
aspgluproasnvaltrpproproleumethisvalleumetglugly
515520525
leuthrargvalproalathrpheglygluseraspproalatrpleu
530535540
glytyrglyaspasnserthrasnvalalaglyalaglyglyglutyr
545550555560
gluvalvalgluglypheglytrpthrasnglyvalleuiletrpval
565570575
alaaspglnpheserthrargleulysargproasncysglyaspile
580585590
thralaalalysvalhisalaserglyglyarglysglnserleuala
595600605
glnargseralaleuglumethisproalaaspalaglytrpthrlys
610615620
metpheglyarglysalaalalysargserleualaargthrvalglu
625630635640
leuglyasnglymetval
645
<210>74
<211>694
<212>prt
<213>嗜热毁丝霉(thermothelomycesthermophila)
<400>74
metalaleuarghisalaalathralaalaleualaglyleuserser
151015
seralaalaalaleutyrileasnglyservalthralaprocysasp
202530
serproiletyrcyshisglygluleuleulysglyvalgluleuala
354045
hisprophevalaspserlysthrphevalaspmetprothrleulys
505560
provalaspgluvalleualaalapheserlysleuargglnproleu
65707580
serasnasnsergluleuasnasnpheleualaglutyrphealapro
859095
alaglyhisgluleuglugluvalprolysglygluleuglnileasp
100105110
prolyspheleuasnlysleugluaspargthrilelysglupheval
115120125
serlysvalileaspiletrpproaspleuthrargargtyralagly
130135140
proglyaspcysserglycysalaasnserpheileprovalasnarg
145150155160
thrphevalvalalaglyglyargphearggluprotyrtyrtrpasp
165170175
sertyrtrpileleugluglyleuleuargthrglyglyalaphethr
180185190
glnileserlysasnileilegluasnpheleuasppheileaspthr
195200205
ileglypheileproasnglyalaargiletyrtyrleuasnargser
210215220
glnproproleuleuthrargmetvallyssertyrvalasptyrthr
225230235240
asnaspthrserileleugluargalaleuproleuleuilelysglu
245250255
hisaspphephethrasnasnargservalservalthralaserasn
260265270
glylysthrtyrthrleuhisargtyrhisvalgluasnasnglnpro
275280285
argproglusertyrarggluasptyrilethralaasnasnglyser
290295300
tyrtyralaalaserglyileiletyrprovallysthrproleuasn
305310315320
gluthrglulysalavalleutyrserasnleualaserglyalaglu
325330335
serglytrpasptyrthralaargtrpleuargvalproaspaspala
340345350
alaargaspvaltyrpheproleuargserleuasnvalargglumet
355360365
valprovalaspleuasnserileleutyrgluasngluvalileile
370375380
alaglutyrleuglulysalaglyasnserserglualalysargphe
385390395400
alaseralaalalysglnargserglualamettyrasnleumettrp
405410415
asnalathrhistrpsertyrpheasptyrasnleuthrserasnala
420425430
glnasnilephevalproalaaspgluaspthralaserpheasparg
435440445
tyralaalaproproglyglnglnvalleuphehisvalalaglnleu
450455460
tyrprophetrpthrglyalaalaproalahisleulysserasnpro
465470475480
leualavalglnlysalatyralaargvalserargargleuaspthr
485490495
lyslysglyalailealaalathrasntyrargthrglyglnglntrp
500505510
aspglnproasnvaltrpproproleuglnhisvalleumetglngly
515520525
leuleuasnthrproalathrpheglygluseraspproalatyrgln
530535540
glyvalglnlysleualaleuargleualaglnargtyrleuaspser
545550555560
thrphecysthrtrptyralathrglyglyserthrseraspphepro
565570575
glnleuglnglyvalserproaspalathrglyilemetpheglulys
580585590
tyralaaspseralathrasnvalalaglyglyglyglyglutyrglu
595600605
valvalgluglypheglytrpthrasnglyvalleuiletrpalaala
610615620
aspvalpheglyasnlysleulysargproaspcysglyasnilethr
625630635640
alaalahisthrhisserglualalysargserleuglyaspglygly
645650655
leualaargargalavalgluleuaspprotrpaspalaalatrpthr
660665670
lysmetpheglyargserlysleuargargargglualagluaspval
675680685
arglysargtrpserser
690
<210>75
<211>680
<212>prt
<213>若虫炭疽菌(colletotrichumnymphaeae)
<400>75
metvalasnphealaleualaleualaalavalalavalleuproarg
151015
thralaglnalaleutyrgluasnglyservalvalalaprocysasp
202530
serproiletyrcyshisglyaspileleulysgluvalgluleuala
354045
argpropheseraspserlysthrphevalaspmetproalailelys
505560
serleuglugluileglnalaalapheasnasnleuserlysproleu
65707580
serasnasnthralaleuasnasppheleuargalaasnphealaala
859095
alaglyglygluleulysgluvalprolysaspglnleulysthrasp
100105110
prolyspheleuasplysileasnaspthrvalilelysglupheser
115120125
glnlysvalilealailetrpproaspleuthrargthrtyralagly
130135140
serthrserasncysthrthrcysproasnserpheileprovalasn
145150155160
histhrphevalvalalaglyglyargphearggluprotyrtyrtrp
165170175
aspserphetrpileleugluglyleuleuargthrglyglyalaphe
180185190
thrgluileserlysasnvalilegluasnpheleuaspleuvalglu
195200205
lysleuglyphevalproasnglyalaargiletyrtyrleuasnarg
210215220
serglnproproleuleualaglnmetvalargleutyrvalglutyr
225230235240
thrseraspargserileleugluargalaileproleuleuilelys
245250255
gluhisgluphetrpthrthrasnargthrvalgluvalasnlysasn
260265270
glythrthrtyrthrleuasnglntyrasnvalthrasnthrglnpro
275280285
argproglusertyrarggluasptyrvalthralaserasnalaser
290295300
tyrtyrserthrasnglyileiletyrprogluvalglulysleuasn
305310315320
aspthrglulysalaargvaltyralaasnleualaserglyalaglu
325330335
serglytrpasptyrserserargtrpleuserargproalaaspala
340345350
alaargaspvaltyrpheproleuargserleuasnvalleugluthr
355360365
valprovalgluleuasnserileleutyrtrpasnglumetthrile
370375380
alaalaleuleuasnserthrasnasnglythrglyalavalalatrp
385390395400
thrgluleualathrlysargserglnalamettyraspleumettrp
405410415
asnserthrleusersertyrpheasptyrasnvalthrserglygly
420425430
glnasniletyrileprolysaspaspaspalaserthrleugluthr
435440445
asnthralaglualagluglnglnvalleupheservalserglnphe
450455460
tyrprophetrpthrglyalaalaproserhisleulysasnasnpro
465470475480
tyralavallysglnalatyrglnargvalglulystyrleuaspile
485490495
lyslysglyglyileproalathrasnleulysthrglyglnglntrp
500505510
aspglnproasnvaltrpproproleumethisvalleumetglugly
515520525
leuargargthrproalathrpheglygluseraspproalatrparg
530535540
aspvalglnserleualaleuargleuglyglnargtyrleuaspser
545550555560
thrphecysthrtrptyralathrglyglyserthrsergluthrpro
565570575
glnleuglnglyleuthralaglnservalglythrmetpheglulys
580585590
tyrglyaspasnserthrasnvalalaglyglyglyglyglutyrglu
595600605
valvalgluglypheglytrpthrasnglyvalleuleutrpalaval
610615620
aspvalpheglyasnaspleulysargproaspcysglyasnilethr
625630635640
alaalaasnvalhisproalalysargserleuproglnargalaval
645650655
gluleuaspsertyraspalasertrpthrlyslyspheglyargarg
660665670
alagluglualaalalysargval
675680
<210>76
<211>680
<212>prt
<213>兰花炭疽菌(colletotrichumorchidophilum)
<400>76
metvalasnphealaleualaleualaalailealavalleuprogln
151015
thralaglnalaleutyrgluasnglyserilevalalaprocysasp
202530
serproiletyrcyshisglyaspileleuarggluvalgluleuala
354045
argpropheseraspserlysthrphevalaspmetproalailelys
505560
serleuglugluileglnalaalapheasnasnleuserlysproleu
65707580
serasnasnthralaleuasnasppheleuargalaasnphealaala
859095
alaglyglygluleuthrgluvalprolysaspglnleulysthrasp
100105110
prolyspheleuaspasnileasnaspthrvalilelysglupheser
115120125
glnlysvalilealailetrpproaspleuthrargthrtyrthrgly
130135140
seralaserasncysthrthrcysproasnserpheileprovalasn
145150155160
histhrphevalvalalaglyglyargphearggluprotyrtyrtrp
165170175
aspserphetrpileleugluglyleuleuargthrglyglyalaphe
180185190
thrgluileserlysasnvalilegluasnpheleuaspleuvalasp
195200205
lysleuglyphevalproasnglyalaargiletyrtyrleuasnarg
210215220
serglnproproleuleualaglnmetvalargleutyrvalglutyr
225230235240
thrasnaspargserileleuglyargalaileproleuleuilelys
245250255
gluhisgluphetrpmetthrasnargthrvalgluvalasnargasn
260265270
glythrthrtyrserleuasnglntyrasnvalthrasnthrglnpro
275280285
argproglusertyrarggluasptyrvalthralaserasnalaser
290295300
tyrtyrserthrserglyileiletyrprogluvalglulysleuasn
305310315320
aspthrglulysalaargleutyralaasnleualaserglyalaglu
325330335
serglytrpasptyrthrserargtrpleuserargproglyaspala
340345350
alaargaspvaltyrpheproleuargserleuasnvalleugluthr
355360365
valprovalgluleuasnserileleutyrtrpasnglumetthrile
370375380
alaalaleuleuasnserthrglnasnglythrglyalaleuthrtrp
385390395400
thrgluleualavallysargserglnalamettyraspleumettrp
405410415
asnserthrleusersertyrpheasptyrasnvalserserglyala
420425430
glnasniletyrileproargaspaspaspalathrgluvalgluthr
435440445
asnthralaglyserglnglnglnvalleupheservalserglnphe
450455460
tyrprophetrpthrglyalaalaproserhisleulysasnasnpro
465470475480
tyralavallysglnalatyrglnargvalglulystyrleuaspasn
485490495
lyslysglyglyileproalathrasnleulysthrglyglnglntrp
500505510
aspglnproasnvaltrpproproleumethisvalleumetglugly
515520525
leuarglysthrproalathrpheglygluseraspproalatrparg
530535540
aspvalglnasnleualaleuargleuglyglnargtyrleuaspser
545550555560
thrphecysthrtrptyralathrglyglyserthrsergluthrpro
565570575
glnleuglnglyleuthralaglnservalglythrmetpheglulys
580585590
tyrglyaspasnserthrasnvalalaglyglyglyglyglutyrglu
595600605
valvalgluglypheglytrpthrasnglyvalleuleutrpalaval
610615620
aspvalpheglyasnglyleulysargproaspcysglyasnilethr
625630635640
alaalaasnvalhisproalalysargserleuserglnargalaval
645650655
gluleuaspglytyraspalaalatrpilelyslyspheglyargarg
660665670
alagluglualaalaargargleu
675680
<210>77
<211>685
<212>prt
<213>木生锥毛壳(coniochaetaligniaria)
<400>77
metalaleuserargleumetalaalaleualaalaserileserval
151015
valasnalaleutyrvalasnglyservalthralaprocysaspser
202530
proleutyrcysglnglyaspileleulysalailegluleualagln
354045
propheseraspserlysthrphevalaspmetprothrilelyspro
505560
leuaspgluvalilealaalapheasnlysleuthrleuproilethr
65707580
asnasnthrgluleuasnasppheleualaalaasnphealaproala
859095
glyglygluleugluthrvalprothrasnglnleuglnthrasppro
100105110
lyspheleuasplysleuasnaspthrvalilearggluphesergln
115120125
alavalilelysiletrpproaspleuthrargglntyralaglypro
130135140
serasncysthrglycysvalaspserpheileprovalasnargthr
145150155160
phevalvalalaglyglyargphearggluprotyrtyrtrpaspser
165170175
tyrtrpilevalgluglyleuleuargthrglyglyasnphethrala
180185190
ilealaargasnthrilegluasnpheleuaspleuvalgluthrile
195200205
glyphevalproasnglyalaargleutyrtyrleuasnargsergln
210215220
proproleuleuserlysmetvalargsertyrvalasptyrthrasn
225230235240
aspthrserileleugluargalavalproleuleuilelysgluhis
245250255
glnphetrpglnthrasnargthrvalvalvalthralaalaaspgly
260265270
lysasntyrthrleuasnargtyrhisvalasnasnasnglnproarg
275280285
progluserphearggluasptyrilethralaasnasnhissertyr
290295300
tyralaalaserglyileiletyrprogluglylysalaleuasnasp
305310315320
serglulysglnglnleutyralaasnleualathrglyalagluser
325330335
glytrpasptyrglythrargtrpleualaargproasnaspalaala
340345350
lysaspvaltyrpheproleuargserleuasnvalvalasnileval
355360365
provalaspleuasnserileleutyrglyalagluserasnileala
370375380
hispheleuasnglnthrglylysserthrglualatyrlystrpala
385390395400
asnleualaalaglnargserglualametphealaleumettrpasn
405410415
serthrleutrpsertyrpheasptyrasnleuthrserasnalagln
420425430
asniletyrileproalaaspaspaspalailethralagluthrser
435440445
glyalaproalaglytyrglnileleupheaspvalalaglnleutyr
450455460
prophetrpthrglyalaalaproaspglnleulysaspasnproleu
465470475480
alavallysleualapheserargvalsersermetleuasparglys
485490495
alaglyglyileproalathrasnpheglnthrglyglnglntrpasp
500505510
glnproasnvaltrpproproleumethisvalleumetalaglyleu
515520525
leuasnthrproalathrpheglygluaspaspprosertyrglnasp
530535540
valglnalaleualaleuargleuglyglnargtyrleuaspserthr
545550555560
phecysthrtrptyralathrglyglyserthrseralathrprolys
565570575
leuglnglyalaglyalaaspalavalglythrmetpheglulystyr
580585590
alaaspasnalathrasnvalalaglyglyglyglyglutyrthrval
595600605
valgluglypheglytrpserasnglyvalleuleutrpalaalaasp
610615620
thrphealaasplysleulysargproaspcysglyasnilethrala
625630635640
alaasnvalasnproglylysargservalargseralavalgluleu
645650655
aspalaargaspalalystrpthrlysmetpheglyservalalaarg
660665670
argglnalaglulysargaspalaglulysargthrtyr
675680685
<210>78
<211>695
<212>prt
<213>土生梭抱壳霉(thielaviaterrestris)
<400>78
metalaproargserphevalalaalaalaalaleualaglyleuile
151015
serseralaseralaleutyrileasnglyservalthralaprocys
202530
aspserproleutyrcyshisglygluileleulysalailegluleu
354045
alahisprophethraspserlysthrphevalaspmetprothrile
505560
argproleuaspgluvalilealaalapheasnargleuserglnpro
65707580
leuserasnasnsergluleuasnalapheleualaalaasnpheala
859095
proalaglyglygluleuglualavalproargaspglnleuhisthr
100105110
gluproserpheleuasnlysleuaspaspthrvalilelysgluphe
115120125
valalalysvalileaspiletrpproaspleuthrargargtyrgly
130135140
glyproglyasncysthralacysalaasnserpheileprovalasn
145150155160
argthrphevalvalalaglyglyargphearggluprotyrtyrtrp
165170175
aspsertyrtrpileleugluglyleuleuargthrglyglyalaphe
180185190
thrgluileserlysasnileilegluasnpheleuaspphevalglu
195200205
thrileglypheileproasnglyalaargiletyrtyrleuasparg
210215220
serglnproproleuleualaargmetvalargsertyrvalasptyr
225230235240
thrasnaspthrserileleuaspargalaleuproleuleuilelys
245250255
gluhisgluphetrpserthrasnargservalserilelysalapro
260265270
asnglylysthrtyrthrleuasnargtyrtyrvalasnasnasngln
275280285
proargprogluserphearggluasptyrilethralaasnasngly
290295300
sertyrtyralaalaserglyileiletyrprovalasnthrproleu
305310315320
asnaspthrglulysalagluleutyralaasnleualaserglyala
325330335
gluthrglytrpasptyrserthrargtrpleulysasnproasnasp
340345350
alaalalysaspiletyrpheproleuargserleuasnvalarggly
355360365
thrvalprovalaspleuasnserileleutyrgluasngluvalile
370375380
ileserglntyrleulysargalaglyasnasnserglualagluarg
385390395400
trpalatyralaalaserglnargserglualametphegluleumet
405410415
trpasnalathrhistrpsertyrpheasptyrasnleuthrserasn
420425430
serglnargilephevalprovalaspaspaspalathralaalaglu
435440445
argalaglyalaproargglyglnglnvalleupheasnileglygln
450455460
phetyrprophetrpthrglyalaalaproalaglnleulysasnasn
465470475480
proleualavalglnglnalatyralaargvalalaargmetleuasp
485490495
gluasnalaglyglyileproalathrasnphevalthrglyglngln
500505510
trpaspglnproasnvaltrpproproleuglnhisvalleumetglu
515520525
glyleuleuasnthrproprothrpheglyaspalaaspproalatyr
530535540
glnservalargalaleualaleuargleualaglnargtyrleuasp
545550555560
serthrphecysthrtrptyralathrglyglyserthrserglnthr
565570575
proglnleuglnglyvalalaproglyalagluglyilemetpheglu
580585590
lystyralaaspasnserthrasnvalalaglyserglyglyglutyr
595600605
gluvalvalgluglypheglytrpserasnglyvalleuiletrpala
610615620
alaaspvalpheglyalaglnleulysargproaspcysglyasnile
625630635640
thralaalahisthrserglyserglyalaglnlysargserglygly
645650655
serleualaargargalavalgluleuaspprotrpaspalaalatrp
660665670
thrlysmetpheglyargseralaleulyslysarggluaspvalarg
675680685
lysargtrpleuleualaala
690695
<210>79
<211>682
<212>prt
<213>足马杜拉分枝菌(madurellamycetomatis)
<400>79
metvalproargleuservalthralaalaleualaalaphealaser
151015
seralaalaalaleutyrileasnglyservalilealaprocysasp
202530
serproleutyrcysglnglygluileleuhisalailegluleuala
354045
argpropheseraspserlysthrphevalaspmetprothrilearg
505560
proleuglugluvalilealaalapheserargleuthrglnproleu
65707580
serasnasnsergluleuasnasppheleualaalaasnphealapro
859095
alaglyglygluleuglugluvalprogluaspgluleuhisthrasp
100105110
provalpheleuaspasnleuaspaspalavalileargglupheval
115120125
glyglnvalileaspiletrpproaspleuthrargargtyralagly
130135140
proglyasncysserglucysalaasnserpheileprovalasnarg
145150155160
thrphevalvalalaglyglyargphearggluprotyrtyrtrpasp
165170175
sertyrtrpileleugluglyleuleuargthrglyglyalaphethr
180185190
gluileserargasnileilegluasnpheleuaspphevalgluthr
195200205
ileglyhisvalproasnglyalaargvaltyrtyrleuasnargser
210215220
glnproproleuleuthrleumetvallysthrtyrvalasptyrthr
225230235240
asnaspthrserileleugluargalaleuproleuleuileargglu
245250255
hisgluphetrpthrasnasnargthrvalaspvalthralaserasp
260265270
glythrthrtyrargleuasnargtyraspvalserasnthrglnpro
275280285
argproglusertyrarggluasptrpvalthralaasnasnalaser
290295300
tyrtyralaalaserglyileiletyrprovalargthrproleuasn
305310315320
glusergluilealagluleutyrserasnleualaserglyalaglu
325330335
serglytrpasptyrthrserargtrpleumetargproasnaspala
340345350
alaargaspvaltyrpheproleuarghisleuasnvalargasnmet
355360365
valprovalaspleuasnserileleutyrglnasngluvalileile
370375380
serglytyrleugluglnalaglyasnserthrglualagluargphe
385390395400
alaseralaalaarggluargserglualamettyralaleumettrp
405410415
asngluthrhistrpsertyrpheasptyrasnleuthrserasnglu
420425430
glnargilephevalprothraspalaasnalathrthralagluleu
435440445
alaglyalaprogluglytyrglnvalleupheaspilealaglnleu
450455460
tyrprophetrpthrglyalaalaproaspglnleulysasnasnpro
465470475480
leualavalglnglnalatyrserargvalalaargmetleuaspglu
485490495
lysproglyalailealaalathrasnphevalthrglyglnglntrp
500505510
aspglnproasnvaltrpproproleuglnhisileleumetglngly
515520525
leuileasnthrproalathrpheglygluaspaspproalatyrgln
530535540
alavalglnaspleuserleuargleualaglnargtyrleuaspser
545550555560
thrphecysthrtrptyralathrglyglyserthrsergluthrpro
565570575
glnleuglnglyvalserproaspalathrglythrmetpheglulys
580585590
tyrseraspalaalaileasnvalalaglyglyglyglyglutyrglu
595600605
valvalgluglypheglytrpthrasnglyvalleuiletrpalaala
610615620
aspthrphealaglyargleuthrargproaspcysglyaspileglu
625630635640
alaalaglnthrhisserglygluargargalavalgluleuasppro
645650655
argaspalaalatrpthrlyslyspheglylysargalaleulyslys
660665670
argalaglusergluarglyslysargval
675680
<210>80
<211>692
<212>prt
<213>粗糙脉孢菌(neurosporacrassa)
<400>80
metvalserargpheleuglyalathrvalproleualaalaalaile
151015
leuproglyalaargalaleutyrvalasnglyservalthralapro
202530
cysaspserproiletyrcystyrglygluleuleuhisglnvalglu
354045
leualaargpropheseraspserlysthrphevalaspmetprothr
505560
ilelysprovalaspgluvalleuglualapheserlysleuthrleu
65707580
proleuserasnasnsergluleuhisglupheleuserthrtyrphe
859095
glyproalaglyglygluleuglualavalprothraspglnleuhis
100105110
valserprothrpheleuaspasnvalseraspaspvalilelysgln
115120125
phevalaspservalileasniletrpproaspleuthrarglystyr
130135140
valglyalaglygluleucysthrglycysalaaspserpheilepro
145150155160
valasnargthrphevalvalalaglyglyargphearggluprotyr
165170175
tyrtrpaspserphetrpileleugluglyleuleuargthrglygly
180185190
alaphethrgluileserlysasnileilegluasnpheleuaspleu
195200205
valgluglnileglyphevalproasnglyalaargleutyrtyrleu
210215220
aspargserglnproproleuleuthrglnmetvalargiletyrval
225230235240
gluhisthrasnaspthrserileleugluargalavalprovalleu
245250255
lyslysglutrpglutrptrpthrthrasnargthrvalgluvalthr
260265270
alaaspglylysthrtyrserleuglnargtyrhisvalaspasnasn
275280285
glnproargproglusertyrarggluasptyrilethralaasnasn
290295300
asnsertyrtyralathrserglyileiletyrprogluthrthrpro
305310315320
leuasnaspthrglnlysalaleuleutyralaasnleualasergly
325330335
alagluserglytrpasptyrserserargtrpleulysasnprogly
340345350
aspalaalaargaspvaltyrpheproleuargserleuasnvalleu
355360365
gluilevalprovalaspleuasnserileleutyrglnasngluval
370375380
thrileglylyspheleualaglnglnglyserlysaspglualaglu
385390395400
glutrpalalyslysalaglugluargserglualamettyrlysleu
405410415
mettrpasnserthrleutrpsertyrpheasptyrasnleuthrser
420425430
serserglnasniletyrvalproalaaspproglnvalpheprophe
435440445
gluglnproserglythrprogluglytyrglnvalleupheserval
450455460
asnglnmetpheprophetrpthrglyalaalaproaspglnleulys
465470475480
glyasnproleualavallysleualaphegluargilelysasnleu
485490495
leuaspasnlysalaglyglyileproalathrasnphevalthrgly
500505510
glnglntrpaspgluproasnvaltrpproproleumethisvalleu
515520525
metaspglyleuleuasnthrproalathrpheglygluaspasppro
530535540
alatyrglngluthrglnthrleualaleuargleualaglnargtyr
545550555560
valaspserthrphecysthrtrptyralathrglyglyserthrser
565570575
gluthrprolysleuglnglyleuglyseraspleulysglyilemet
580585590
pheglulystyrseraspasnserthrasnvalalaglyserglygly
595600605
glutyrgluvalvalgluglypheglytrpthrasnglyvalleuile
610615620
trpalaalaasplyspheglyasplysleulysargproaspcysgly
625630635640
aspilethrproalaglnvalglylysargalaaspilethrmetglu
645650655
lysargalavalgluleuaspvalpheaspalalysphethrlyslys
660665670
phealaarglysglylysleuglulysleulysalalysphelysarg
675680685
argalaalaile
690
<210>81
<211>682
<212>prt
<213>大丽轮枝菌(verticilliumdahliae)
<400>81
metthrleuleuserlysservalvalalaleualaalaalaalapro
151015
leualaargalaglnleupheargasnglyservalilealaprocys
202530
aspseralailetyrcyshisglygluileleuserglnileglnleu
354045
alaargprophegluaspserlysthrphevalaspmetproalalys
505560
argserleugluglnileglnglualapheaspgluleuglulyspro
65707580
leuthrasnasnthralaleuasnasppheleualaalaasnpheala
859095
leualaglyglygluleusergluvalaspaspleugluthraspala
100105110
glnpheleuaspasnileasnaspthrvalilearggluphethrglu
115120125
lysvalilegluiletrpproaspleuthrargsertyrthrglyala
130135140
glyserasncysthraspcysproasnserpheileproileasnarg
145150155160
thrphevalvalalaglyglyargphearggluprotyrtyrtrpasp
165170175
serphetrpileleugluglyleuleuargthrglyglyserpheile
180185190
gluvalserargasnhisilegluasnpheleuaspleuvalaspgln
195200205
tyrglyphevalmetasnglyalaargargtyrtyrleuasnargser
210215220
glnproproleuleuserglnmetvalargleutyrvalasphisthr
225230235240
asnaspthraspileleuaspargalaleuproleuleuilelysglu
245250255
hisglutrptrpthrvalasnargthrvalgluvalserlysaspgly
260265270
argthrtyrglnleuasnleutyrasnvalserasnthrglnproarg
275280285
proglusertyrtyrgluasptyrthrthralaserasnsersertyr
290295300
tyralagluserglyileiletyrprogluthrarggluleuasnasp
305310315320
thrglulysalapheleutyralaasnleualaserglyalagluser
325330335
glytrpasptyrthrserargtrpleuserlysproasnaspalaval
340345350
arghisvaltyrpheproleuargserleuasnilevalasnileval
355360365
provalgluleuasnserileleutyrtrpasngluvalthrileala
370375380
glytyrleuasnglnthrserasngluthrglnalaalaglutrpthr
385390395400
gluleualaalaasnargserglualamettyrasnleumettrpasn
405410415
aspthrleuserglytyrpheasptyrasnleuthrserglyalagln
420425430
aspthrphevalproalaaspaspaspthrthraspleuglualathr
435440445
glyalaprogluglyglnglnvaltyrpheservalalaglnphetyr
450455460
prophetrpthrglyalaalaproalahisilelysasnasnproleu
465470475480
alavallysthralaphegluargvalalathrtyrleuaspthrlys
485490495
aspglyglyileproalathrasnleuargthrglygluglntrpasp
500505510
glnproservaltrpproproleumethisileleumetglualaleu
515520525
argasnthrproalathrpheglygluseraspprohistyrmetglu
530535540
valglnaspleualaleuargleuglyglnargtyrleuaspserthr
545550555560
phecysthrtrptyralathrglyglyserthrseraspthrprogln
565570575
leugluglypheaspgluarggluvalglyilemetpheglulystyr
580585590
seraspasnserthrasnglnalaglyglyglyglyglutyrgluval
595600605
valgluglypheglytrpthrasnglyvalleuleutrpvalvalasp
610615620
thrpheglyasnasnleuthrargproaspcysglyaspilegluala
625630635640
alaasnvalglnproserlysargseralavalgluleuhisargarg
645650655
aspalaalatrpthrlyslyspheglyserargseralalysglyglu
660665670
alalysargphealalysleuargglytyr
675680
<210>82
<211>688
<212>prt
<213>玉蜀黍赤霉(gibberellazeae)
<400>82
metproserproarghisilealaalaalaleuvalalathralathr
151015
thralaseralaleutyrvalasnglythrvalvalalaprocysasp
202530
serproiletyrcyshisglyaspileleugluglnvalgluleuala
354045
argpropheseraspserlysthrphevalaspmetproalailearg
505560
proleuseraspileglnglualapheasplysleuglulysproleu
65707580
argasnasnseralaleualaasppheleuaspgluasnpheaspasp
859095
alaglyasngluleuglugluvalserargaspaspleuaspthrasp
100105110
prolyspheleuaspasnileasnaspthrvalilearggluphethr
115120125
glulysvalileaspiletrpproaspleuthrargargtyraspgln
130135140
aspalalysasncysseraspcysproasnserpheileprovalasn
145150155160
argserphevalvalalaglyglyargphearggluprotyrtyrtrp
165170175
aspsertyrtrpileileleuglyleuleuargthrglyglyserphe
180185190
ilegluilealalysasnthrilegluasnpheleuasppheileglu
195200205
glutyrglyphevalproasnglyalaargiletyrtyrleuasnarg
210215220
serglnproproleuleuserglnmetilelysalatyrvalglutyr
225230235240
thrasnaspthraspileleugluargalaleuproleuleuvalgln
245250255
gluhisgluphephemetasnasnargservalprovaltyrileasn
260265270
asngluthrtyrtyrleuasnthrtyrasnvalserasnthrargpro
275280285
argproglusertyrarggluasptyrilethralagluasnthrser
290295300
tyrtyrserprogluserglylysvaltyrlysglyglyglugluleu
305310315320
serphelysglnlysglualaleutyrglyasnleualaserglyala
325330335
gluserglyleuasptyrthrvallystrpilealaargprogluasp
340345350
alaileargaspasntyrpheproleuargtyrleuasnthrargasn
355360365
ileileprovalaspleuasnserileleutyrglyasngluileala
370375380
ilealaaspphetyrgluglnthrglyasnasnseralaserglugln
385390395400
trparggluvalalaalaasnargsertyralamethisglypheleu
405410415
trpasngluthrleutrpsertyrpheasptyrasnleuthrserlys
420425430
alaglnglniletyrpheprovalaspgluasnthrthrvalvalasp
435440445
thrgluaspalaprolysglyglnglnvalphepheserprothrgln
450455460
phetyrprophetrpleuglyalaalaproasptyrleulysasnasn
465470475480
prophealavaltyrthralatyrlysargvalglutyrtyrleuasp
485490495
asnargaspglyglyileproalaserasnvalgluthrglyglngln
500505510
trpaspglnproasnvaltrpproproleumethisileleumetala
515520525
glyleugluargvalproprothrpheglyileargaspproserphe
530535540
valgluvalargargleualaleuargleuglyglnargtyrleuasp
545550555560
serthrphecysthrtrptyralathrglyglyserthrsergluthr
565570575
prolysleuglnserthrseraspgluglugluglyilemetpheglu
580585590
lystyralaaspasnalathrasnvalalaglyglyglyglyglutyr
595600605
gluvalvalgluglypheglytrpthrasnglyvalleuiletrpala
610615620
valaspgluphelysasnargleuthrargprolyscysaspasnleu
625630635640
gluseralahisserasnasplysargaspproseralavalmetleu
645650655
asnalaargaspalalysargvallyslyspheglyargarglysarg
660665670
alaalaglulysalaalalyslysargserserargvalphetyrphe
675680685
<210>83
<211>15
<212>prt
<213>乳酸克鲁维酵母(kluyveromyceslactis)
<400>83
glnprotyrvalalaasnglytyrileglyserargileproasn
151015
<210>84
<211>17
<212>prt
<213>乳酸克鲁维酵母(kluyveromyceslactis)
<400>84
glyvalalaglyleuserseraspsertyrglyglymetvalphetrp
151015
asp
<210>85
<211>20
<212>prt
<213>乳酸克鲁维酵母(kluyveromyceslactis)
<400>85
asnilethrleuglutyrserglymetasnserservalgluilelys
151015
glnalaaspval
20
<210>86
<211>13
<212>prt
<213>乳酸克鲁维酵母(kluyveromyceslactis)
<400>86
asnilethrleuglutyrserglymetasnserserval
1510
<210>87
<211>698
<212>prt
<213>稻瘟病菌(magnaporthegrisea)
<400>87
metserproleutrplysthralaalaalailealavalalaalaser
151015
glyserleuvalasnalavaltyrileasnglyserileilethrpro
202530
cysaspserleuiletyrcysargglygluleuleulysgluvalglu
354045
leualahisprophealaaspserlysthrphevalaspmetprothr
505560
ilelysprovalaspgluvalileglualapheasnlysleuglnlys
65707580
proleuserasnasnthrgluleuglnasppheleuarggluasnphe
859095
alaglnalaglyglygluleuglugluvalproasnsergluleuglu
100105110
thraspprovalpheleuasplysleuaspaspthrvalileargglu
115120125
phevalglulysvalileaspiletrpproserleuthrargargtyr
130135140
lysglyproserasncysglualacysalaaspserpheileproval
145150155160
asnargthrphevalvalalaglyglyargphearggluprotyrtyr
165170175
trpaspsertyrtrpileleugluglyleuleuargthrglyglyala
180185190
phethrasnileserlysasnthrvalgluasnpheleuaspleuval
195200205
gluthrileglyphevalproasnglyalaargiletyrtyrlysasn
210215220
argserglnproproleuleuserglnmetvalargiletyrvalglu
225230235240
histhrasnaspthrserileleuglyargalavalproleuleuile
245250255
lysgluhisgluphepheileasnasnargserileaspvalgluala
260265270
serasnglylysthrtyrargleuglnargtyralavalthrasnthr
275280285
glnproargproglusertyrarggluasptyrilethralaserasn
290295300
argsertyrtyrserproserglyileiletyrprogluserhisgln
305310315320
leuasngluserglulysalavalleutyrserhisleualasergly
325330335
alagluserglytrpasptyrthrserargtrpleuserthrproser
340345350
aspalavalargaspasntyrpheproleuargserleuasnthrasn
355360365
asnilevalprovalaspleuasnserileleutyralaasngluval
370375380
alailealaglupheleuasnargthrglyasnserthrglyalaser
385390395400
asptrpmetaspleualalysglnargserglualamettyralaleu
405410415
mettrpasngluthrleutrpsertyrpheasptyrasnmetthrser
420425430
lysthrglnasnargpheileprovalaspgluaspalavalserile
435440445
gluthrasnasnalaproalaglyglnglnvalphephehisvalala
450455460
glntyrtyrprophetrpthrglyalaalaproargserleulysasn
465470475480
asnproleualavalleuargalatyrgluargileaspalatyrleu
485490495
aspilelysargglyalaileproalathrasnleulysthrglygln
500505510
glntrpaspgluproasnvaltrpproproleumethisileleumet
515520525
gluglyleuthrargvalproalathrpheglygluaspaspvalala
530535540
trpthrgluileglnaspleualaleuargleuglyglnargtyrleu
545550555560
aspserthrphecysthrtrptyralathrglyglyserthrserglu
565570575
thrproglnleuglnglyleuasnalagluasplysglyilemetphe
580585590
glulystyrglyaspasnserthrasnvalalaglyserglyglyglu
595600605
tyrgluvalvalgluglypheglytrpthrasnglyvalleumettrp
610615620
valalaaspthrpheasnasnlysleuthrargproaspcysglyasn
625630635640
ilethralaalaasnvalhisseraspglyserglnalaarglysarg
645650655
glyglumettrpseralaleuglumethisprotyraspalaalatrp
660665670
thrlysglupheglyalaarglysvalargargasplysalagluala
675680685
argalaleuglyasnvalmetglyglyval
690695
<210>88
<211>676
<212>prt
<213>稻瘟病菌(magnaporthegrisea)
<400>88
valtyrileasnglyserileilethrprocysaspserleuiletyr
151015
cysargglygluleuleulysgluvalgluleualahispropheala
202530
aspserlysthrphevalaspmetprothrilelysprovalaspglu
354045
valileglualapheasnlysleuglnlysproleuserasnasnthr
505560
gluleuglnasppheleuarggluasnphealaglnalaglyglyglu
65707580
leuglugluvalproasnsergluleugluthraspprovalpheleu
859095
asplysleuaspaspthrvalilearggluphevalglulysvalile
100105110
aspiletrpproserleuthrargargtyrlysglyproserasncys
115120125
glualacysalaaspserpheileprovalasnargthrphevalval
130135140
alaglyglyargphearggluprotyrtyrtrpaspsertyrtrpile
145150155160
leugluglyleuleuargthrglyglyalaphethrasnileserlys
165170175
asnthrvalgluasnpheleuaspleuvalgluthrileglypheval
180185190
proasnglyalaargiletyrtyrlysasnargserglnproproleu
195200205
leuserglnmetvalargiletyrvalgluhisthrasnaspthrser
210215220
ileleuglyargalavalproleuleuilelysgluhisgluphephe
225230235240
ileasnasnargserileaspvalglualaserasnglylysthrtyr
245250255
argleuglnargtyralavalthrasnthrglnproargprogluser
260265270
tyrarggluasptyrilethralaserasnargsertyrtyrserpro
275280285
serglyileiletyrprogluserhisglnleuasngluserglulys
290295300
alavalleutyrserhisleualaserglyalagluserglytrpasp
305310315320
tyrthrserargtrpleuserthrproseraspalavalargaspasn
325330335
tyrpheproleuargserleuasnthrasnasnilevalprovalasp
340345350
leuasnserileleutyralaasngluvalalailealaglupheleu
355360365
asnargthrglyasnserthrglyalaserasptrpmetaspleuala
370375380
lysglnargserglualamettyralaleumettrpasngluthrleu
385390395400
trpsertyrpheasptyrasnmetthrserlysthrglnasnargphe
405410415
ileprovalaspgluaspalavalserilegluthrasnasnalapro
420425430
alaglyglnglnvalphephehisvalalaglntyrtyrprophetrp
435440445
thrglyalaalaproargserleulysasnasnproleualavalleu
450455460
argalatyrgluargileaspalatyrleuaspilelysargglyala
465470475480
ileproalathrasnleulysthrglyglnglntrpaspgluproasn
485490495
valtrpproproleumethisileleumetgluglyleuthrargval
500505510
proalathrpheglygluaspaspvalalatrpthrgluileglnasp
515520525
leualaleuargleuglyglnargtyrleuaspserthrphecysthr
530535540
trptyralathrglyglyserthrsergluthrproglnleuglngly
545550555560
leuasnalagluasplysglyilemetpheglulystyrglyaspasn
565570575
serthrasnvalalaglyserglyglyglutyrgluvalvalglugly
580585590
pheglytrpthrasnglyvalleumettrpvalalaaspthrpheasn
595600605
asnlysleuthrargproaspcysglyasnilethralaalaasnval
610615620
hisseraspglyserglnalaarglysargglyglumettrpserala
625630635640
leuglumethisprotyraspalaalatrpthrlysglupheglyala
645650655
arglysvalargargasplysalaglualaargalaleuglyasnval
660665670
metglyglyval
675
<210>89
<211>1109
<212>prt
<213>光滑假丝酵母(candidaglabrata)
<400>89
serthrasnsertyrpheserserserleualaseralaserproleu
151015
pheasnlysgluglyargvalvalargprometarggluserasnleu
202530
glyleuhisalaaspproglnthrarglysserserlysthrleutyr
354045
aspleuleuserasppheaspasnalaphetyraspaspgluasnmet
505560
ileleuglyserleualapheglygluasnthrtyrserargglnpro
65707580
tyrvalalaasnglytyrileglyserargileproasnileglyphe
859095
glytyralaleuaspthrleuasnleutyralaaspalaproglyala
100105110
leuasnasnglytrpproleuargasnargargphealaglyserphe
115120125
valseraspphetyrserleuglnalalysleuasnserthrasnphe
130135140
progluleuaspglulysglytyrthrthrvalileserserilepro
145150155160
glutrpthraspleuglnphethrvalaspleuasnglythrasntrp
165170175
pheasnproglnservalleuileaspaspvalileasntyrasngln
180185190
asnleusermetlysaspglyilevalserthrasnmetasptrpleu
195200205
asnglymetileasnilelyssergluvaltrpalahisarglysile
210215220
hisserleuglyilethrargleugluileserleuasnleuaspala
225230235240
leuproaspgluphethrgluleuprovalthriletyraspileile
245250255
asppheasnthrserhisargthrthrleutyrglulysglyglnasp
260265270
gluaspasnlysalailetyrmetilevalasnprogluasnvalpro
275280285
tyrserasnalavalvaltyrserthrcysthrilelysglythrglu
290295300
asnasnpheserprotyrasnphethrseraspaspargilealaarg
305310315320
asntyrmetthrasnleuthrglugluasnprolysvalvaliletyr
325330335
lystyrthrservalvalserserglutyrasnasnaspgluproasn
340345350
proasnvalasnleulysphealaserasnilealaasnthralalys
355360365
glyasntyrlysserleuleuserasnhislysargalatrptyrasp
370375380
leutyrasnaspalapheilegluileproseraspserleuleuglu
385390395400
metthralaargserserleuphehisleuleualaasnthrarggln
405410415
tyrasnvalserthrthrargglyleuprovalglyvalglyglyleu
420425430
serseraspsertyrglyglymetvalphetrpaspalaaspvaltrp
435440445
metalaproalaleuleuprophepheproasnilealametasnmet
450455460
asnasntyrargasnalathrhisglnglnalailegluasnalalys
465470475480
glntyrasntyrproglyalavaltyrprotrpthrserglyargtyr
485490495
alaasncysthrserthrglyprocysileasptyrglutyrhisile
500505510
asnvalaspilealaleualaserpheseriletyrmetasnglyala
515520525
gluglyalaaspgluasptyrleuargphethrthrtrpprometval
530535540
lysaspalaalavalphephelysalatyrvallystyrasngluthr
545550555560
leuglyglutyrgluthrtyrasnleuthraspproaspglupheala
565570575
asnhisvalasnasnglyalaphethrasnalaglyilelysthrleu
580585590
leulystrpalathraspileglythrhisleuglyglugluvalasp
595600605
prolystrpmetgluilealaaspasnilehisileproargserasp
610615620
serasnilethrleuglutyrserglymetasnserservalgluile
625630635640
lysglnalaaspvalthrleumetvaltyrproleuglytyrileasn
645650655
aspgluserileleuasnasnalailelysaspleutyrtyrtyrser
660665670
gluargglnseralaserglyproalametthrtyrprovalpheval
675680685
alaalaalaalaserleuleuasnhisglyserserserglnsertyr
690695700
leutyrlysservalleuprotyrleuargserprophealaglnphe
705710715720
sergluglnseraspaspasnpheleuthrasnglyleuthrglnpro
725730735
alaphepropheleuthralaasnglyglypheleuglnserileleu
740745750
pheglyleuthrglyleuargtyrsertyrgluvalthrproargthr
755760765
lyslysileserargleuleulyspheaspprovallysleuproleu
770775780
leuproglyglyilealaileargasnphelystyrmetglyglnval
785790795800
leuaspileileileaspaspasnasnglythrilealahislysgly
805810815
glyasplysproileargilelysvalproasnargaspileleuhis
820825830
aspargasnilethrseralaleutyrserlysargaspaspaspleu
835840845
seralathraspasptyrtyrglythrtyrphethrleutyrproasn
850855860
glugluleuvalileproleutyraspthrlysleuasnileaspgly
865870875880
asnilealagluserlysglnilethrasnleuthralaglyvalpro
885890895
glyaspvalglypheseralaleuaspglyasnasntyrthrhistrp
900905910
glnpropheasplysseraspasnalalysleuleuileaspleugly
915920925
pheasnserthrhisvalilelyslysglyileileleutrpglygln
930935940
argproalalysasnileserleuservalleuprohissergluarg
945950955960
ilegluglnleuphealaasnilethraspleuleugluthrserser
965970975
ilethrlysglyglyserleuleuasnglnmetleuglyglnthrgln
980985990
serasnvalthralagluileaspaspaspileleualaleuleuasn
99510001005
trplysglyaspaspleuaspglnleuileprotyrleuproasp
101010151020
methisleuleuglnglulyspheileproileleulysasptyr
102510301035
proilelysproasnglnargtyrtyrlysgluileileaspasp
104010451050
aspileilelysleuleuproserasnthrthrgluphethrile
105510601065
asptyrasnserileproglyglyglulysargalaargtyrval
107010751080
valleuthrvalhisglythrtyraspaspaspaspaspleulys
108510901095
glyalathrilelysgluilevalleuglnglu
11001105
<210>90
<211>1020
<212>prt
<213>近平滑假丝酵母(candidaparapsilosis)
<400>90
argpheserglualapheprophevalleuproargalaglulystyr
151015
thrleugluglnthrseraspserasnleuglyaspphevalasnser
202530
phegluasnlysgluilepheserglnvalglnphesergluhisala
354045
phetyrasparglysmetasnvalvalglythrileglutyrserlys
505560
tyrasnglnpheglnlysglnprotyrvalalaasnglytyrilegly
65707580
serargileproasnleuglyhisglyphethrtyraspglnleuthr
859095
gluserproaspserlyslysaspaspleutyrasnglytrpproleu
100105110
pheasplysargpheserglyalapheilealaglyphetyraspleu
115120125
glngluasnthrthrglyasnasnpheprogluleuleulysasngly
130135140
tyrgluservalileseralaileproglntrpthrthrleulysleu
145150155160
alaileglnhisglyasplysargtyrthrleuaspproalaserpro
165170175
aspglyvalglyglnilethrasntyrvalglnasnmetserleuser
180185190
thrglyilevalthrthrglnphethrtrpleugluserleuaspval
195200205
argtyrthrvalleualahisargsergluileasnleuglyleuval
210215220
lysvalaspilelysasnlysglyasnaspthrvalgluileaspval
225230235240
ileaspgluleuasnpheaspthralaglnargcysserleuasnleu
245250255
ileglyhisaspasnserglyiletyrvalserpheserproaspasn
260265270
leuasptyrileserglyalailetyrserthrleuhisleuaspser
275280285
vallysarghisserlysasnlyslysthrasnglnthrserserile
290295300
argleuhisalaglnaspserthrglnpheglylysthrvalglyile
305310315320
valserthraspleuaspproserlysphelysasnserargaspthr
325330335
leugluphealalyslysvalserglnlyspheglutyrleuaspleu
340345350
valilelysserhisvalargalatrplysgluleumetglylysgln
355360365
serthrilethrpheproasnaspproleuleuaspleuglyserlys
370375380
alaservaltyrhisleuleualaasnthrargproaspalaglngly
385390395400
leuthrglyalaleuglyvalalaglyleuserseraspsertyrgly
405410415
glymetvalphetrpaspthraspphetrpmetpheargalaileleu
420425430
alaleuaspprothrhisalalysserilevalasntyrargmethis
435440445
thrhisglnglnalaleulysasnthrproglnglytyrgluglyala
450455460
alatyrprotrpthrserglyargpheglyasncysthralathrgly
465470475480
procysleuasntyrglutyrhisileasnseralailealametala
485490495
alatrpgluleutyrileserglyalavalaspaspglntyrleuglu
500505510
serthrvaltyrproleuileseraspalaalalysphetyralaasp
515520525
tyrvalthrsertyrasngluthrileasplyspheilethrserasn
530535540
leuthraspproaspglutyralaasnhisvalaspasnglyalatyr
545550555560
thrasnalaglyilealaleuleumetasntrpileasnaspilegly
565570575
aspileleuglyilelysmetprogluiletyrthraspilesergln
580585590
lysmettyrleuprothralaaspasnserglnasnilethrleuglu
595600605
tyrserglymetasnserservalglyilelysglnalaaspvalval
610615620
leumetthrtyrproleuglnasnserleuvalaspaspgluglnala
625630635640
tyrileasnmetgluphetyrserglylysglnvalsertyrglypro
645650655
alametthrpheserilepheserilevalalaalaasnleualathr
660665670
serglycysalaserglnsertyrleuhislysalametglnprotyr
675680685
leuargglyprophealaglnphesergluglnasnasnaspasnphe
690695700
lysthrasnglyglythrhisproalatyrpropheleuthralahis
705710715720
glyglypheileglnalavalvalasnglyleuthrglyleuarghis
725730735
sertyrthrileaspasnglylysileargargserleupheleuasp
740745750
proilealaleuprocysleuglyaspglyvalglntyrserglyile
755760765
histyraspasnhisthrilesermetasnilethrglyserthrphe
770775780
valileargasngluglylysthrasnlysargalaasnasptyrile
785790795800
serilethrilealaaspargasnproglyargglythrtyrlysleu
805810815
lysaspasngluglulysserphelysleutyrglnproserlysile
820825830
phegluaspservalserglucysglyleualathrphetyrasnile
835840845
thrgluseralaproglyaspserserpheserileasnaspglyasp
850855860
asnthrthrargtrpglnvallystyrasnaspthrthrglylysval
865870875880
leuileaspleuhisserilelysasnilethrglyvalvalpheasn
885890895
trpalaasplysproprolyslysvallysleuserlystyrmetgly
900905910
thrlysphelysaspvalthrasppheleualalysvalaspphegly
915920925
asntyrvaltyrlysasntyrargphealaasnproglyglulysleu
930935940
leuasnglnsergluvalphegluasnvaltyrseraspaspvalthr
945950955960
ileasnalapropheserserlysgluleuglugluvalilevalpro
965970975
valarghisasnthrthrsertrpasnileasnleuglnglyargphe
980985990
leuleuleugluvalaspglyilehisasnthrileproilegluasp
99510001005
asptyrglyglyalalysleualagluvalasnphe
101010151020
<210>91
<211>1126
<212>prt
<213>乳酸克鲁维酵母(kluyveromyceslactis)
<400>91
thrlysproleuproleuglnhisthrhispheglyserphevalcys
151015
alagluaspthrleuphecysprogluserglnarglysalaserglu
202530
lysmettyrserleuleulysasphisgluasnthrphetyraspglu
354045
gluglnglnileleuglyasnleuleuleusergluasnthrpheser
505560
argglnprotyrvalalaasnglytyrileglyserargileproasn
65707580
leuglypheglytyralaleuaspthrileasnvaltrpvalasnasp
859095
serserileproglyalaleuaspasnglytrpproleuargasngln
100105110
argphealaglyalapheileseraspphetyrcysleuglnglulys
115120125
leuasnserthrasnpheprogluleuaspaspaspglytyrserthr
130135140
valileserthrileproglntrpthraspleuserileleulyshis
145150155160
thrthrthrglyglnileglutyrileasnprothraspvallysleu
165170175
asplysilethrasntyrmetglnasnleuserleuglnaspglyile
180185190
valthrthrthrphevaltyrasplysglnleuleuvalthrthrlys
195200205
valvalalahisarglysiletyrproleuglyvalvalthrleuglu
210215220
leuserleupheaspasnasnseraspthrseralaaspasngluasn
225230235240
glutyrvalgluleugluilecysaspserleuasnpheserthrser
245250255
hisargthrvalleuasnasptyrglytyraspglnasnasnglugly
260265270
ilephemetvalvalgluprogluasnvalprotyrserasnalaser
275280285
leuphesertyrpheaspileproserargaspthrleuthrleuser
290295300
lystyrseraspserileserglncysthrthrglnileleulysala
305310315320
asnserthrphevalalahislystyrileglyileileserserglu
325330335
tyraspasnlysglnproaspaspasnserasnglythrserasnglu
340345350
alaleulysmetserasnleugluargalathrserilevalleuasp
355360365
asnlysglyasntyraspserleuileglnserhislysasnalatrp
370375380
lysargiletyrlysaspalaserilegluileproseraspglyleu
385390395400
leuglumetthralalysserseriletyrhisleuleualaasnthr
405410415
argserhisasnvalsergluaspargglyleuproileglyvalser
420425430
glyleuserseraspsertyrglyglymetvalphetrpaspserasp
435440445
leutrpileleuproalaleuleuprophepheproasnalaalaarg
450455460
glnileasnasntyrargasnalaserleuhisglnalalysleuasn
465470475480
alaglulystyrglytyraspglyalaleutyrprotrpthrsergly
485490495
argtyralaasncysthrserthrglyprocysvalasptyrglutyr
500505510
hisileasnvalaspilealaleuserserphealailetyrmetasn
515520525
glyaspgluaspaspgluargsergluglutyrleuargtyrthrthr
530535540
trpprophevalgluasnalaalalysmetphealaglntyrvallys
545550555560
trpasnaspthrmetglnglntyrthrthrhisasnleuthrasppro
565570575
aspglutyralaasnphevalaspasnalaalaphethrasnalagly
580585590
ileglnservalmetvaltrpalahisaspilealaarghisleugly
595600605
ileaspproaspproglntrpleugluilealaaspasnilehisile
610615620
proilesergluthrasnilethrleuglutyrserglymetasnser
625630635640
servalgluilelysglnalaaspvalvalleumetiletyrproleu
645650655
sertyrilethraspglnserileleuasnasnalailelysasnleu
660665670
tyrtyrtyrsergluargglnseralaserglyproalametthrtyr
675680685
provalphevalalaglyalaalaserleuleuasntyrglyserser
690695700
serglnsertyrleutyrlysservalvalprotyrleuargserpro
705710715720
phealaglnphesergluglnseraspaspasnpheleuthrasngly
725730735
leuthrglnproalaphepropheleuthralaasnglyglypheleu
740745750
glnserileleupheglyleuthrglyleuargtyrsertyrgluval
755760765
aspgluaspthrglylysilehisargleuleulyspheasnproile
770775780
glnleuproleuleuproglyglyileargileasnasnphelystyr
785790795800
metglyglnvalleuaspvalleuilethraspthrgluglyileile
805810815
ilehislysasnglythrlysgluileargilelysvalproasparg
820825830
thrleuileproaspvalaspvallystyrserglugluthrasppro
835840845
ilelysglnileleuhisglyargargservalprothrglylysasn
850855860
tyrphethrileglnproglyaspvalphelysthrproleutyrile
865870875880
prolyslysasnleugluglyasnleuvalglualalysglnilethr
885890895
asnleuthralaglyvalproglyaspvalalavalservalileasp
900905910
glyasnasnphethrhistrpglnproalatyrlysasnleuproala
915920925
argleuleuileaspmetglyasnasnphethrglngluilelysser
930935940
glylysileiletrpglyserargproalalysthrpheserleuser
945950955960
ileleuproglnthrglugluvalphelysasnleuthrhisileleu
965970975
serasnvalasnglntyrcysasnlysthrglyaspglucysvalarg
980985990
aspleugluargthrglulysglypheaspalaalailegluaspval
99510001005
pheasptrptyrglyileaspproaspserileileserthrhis
101010151020
progluleulysaspmetlysthrlysphevallysileleuasp
102510301035
histyrlysvalthrprosergluprotyrprotrpargvaltyr
104010451050
asngluserglnilevalleuleuproglyasngluthraspphe
105510601065
aspileasptyrserlysvalalaglumetasnprogluasnval
107010751080
aspileasppheargglyasnglnthrasptrparglysglyarg
108510901095
pheilevalleuthrvalhisaspthrtyraspaspaspaspasp
110011051110
glulysglyalathrilelysgluphealaleuphepro
111511201125
<210>92
<211>695
<212>prt
<213>人工序列(artificialsequence)
<220>
<223>由多种生物体稻瘟病菌和酿酒酵母共同组成的序列
<400>92
metlyspheileserthrpheleuthrpheileleualaalavalser
151015
valthralavaltyrileasnglyserileilethrprocysaspser
202530
leuiletyrcysargglygluleuleulysgluvalgluleualahis
354045
prophealaaspserlysthrphevalaspmetprothrilelyspro
505560
valaspgluvalileglualapheasnlysleuglnlysproleuser
65707580
asnasnthrgluleuglnasppheleuarggluasnphealaglnala
859095
glyglygluleuglugluvalproasnsergluleugluthrasppro
100105110
valpheleuasplysleuaspaspthrvalilearggluphevalglu
115120125
lysvalileaspiletrpproserleuthrargargtyrlysglypro
130135140
serasncysglualacysalaaspserpheileprovalasnargthr
145150155160
phevalvalalaglyglyargphearggluprotyrtyrtrpaspser
165170175
tyrtrpileleugluglyleuleuargthrglyglyalaphethrasn
180185190
ileserlysasnthrvalgluasnpheleuaspleuvalgluthrile
195200205
glyphevalproasnglyalaargiletyrtyrlysasnargsergln
210215220
proproleuleuserglnmetvalargiletyrvalgluhisthrasn
225230235240
aspthrserileleuglyargalavalproleuleuilelysgluhis
245250255
gluphepheileasnasnargserileaspvalglualaserasngly
260265270
lysthrtyrargleuglnargtyralavalthrasnthrglnproarg
275280285
proglusertyrarggluasptyrilethralaserasnargsertyr
290295300
tyrserproserglyileiletyrprogluserhisglnleuasnglu
305310315320
serglulysalavalleutyrserhisleualaserglyalagluser
325330335
glytrpasptyrthrserargtrpleuserthrproseraspalaval
340345350
argaspasntyrpheproleuargserleuasnthrasnasnileval
355360365
provalaspleuasnserileleutyralaasngluvalalaileala
370375380
glupheleuasnargthrglyasnserthrglyalaserasptrpmet
385390395400
aspleualalysglnargserglualamettyralaleumettrpasn
405410415
gluthrleutrpsertyrpheasptyrasnmetthrserlysthrgln
420425430
asnargpheileprovalaspgluaspalavalserilegluthrasn
435440445
asnalaproalaglyglnglnvalphephehisvalalaglntyrtyr
450455460
prophetrpthrglyalaalaproargserleulysasnasnproleu
465470475480
alavalleuargalatyrgluargileaspalatyrleuaspilelys
485490495
argglyalaileproalathrasnleulysthrglyglnglntrpasp
500505510
gluproasnvaltrpproproleumethisileleumetgluglyleu
515520525
thrargvalproalathrpheglygluaspaspvalalatrpthrglu
530535540
ileglnaspleualaleuargleuglyglnargtyrleuaspserthr
545550555560
phecysthrtrptyralathrglyglyserthrsergluthrprogln
565570575
leuglnglyleuasnalagluasplysglyilemetpheglulystyr
580585590
glyaspasnserthrasnvalalaglyserglyglyglutyrgluval
595600605
valgluglypheglytrpthrasnglyvalleumettrpvalalaasp
610615620
thrpheasnasnlysleuthrargproaspcysglyasnilethrala
625630635640
alaasnvalhisseraspglyserglnalaarglysargglyglumet
645650655
trpseralaleuglumethisprotyraspalaalatrpthrlysglu
660665670
pheglyalaarglysvalargargasplysalaglualaargalaleu
675680685
glyasnvalmetglyglyval
690695
<210>93
<211>4884
<212>dna
<213>人工序列(artificialsequence)
<220>
<223>由多种生物体酿酒酵母和小孢根霉共同组成的序列:
<400>93
ctaaattcggccttgctcagagactcctggattttggctaacaacgcagtcccttcgatg60
catatagctaggccacaaattatgccaataacggtccatgggttgatgttttcttgaatt120
ctttcgtttttcatgctatttgcgtcttcccaagtcccagcgttccagtattcatactgc180
gcgttagagtggtagccataagagccggcatattggtaattttcagtattaacgttagaa240
cgtggtgaatacgatgtggtccagccttgcctcgttgtgtcatatacgatctttttcttt300
gggtcacaaagaatatcatatgcttgagagatgactttaaatctatgtagtttttcgctt360
gatgttagcagcagcggtgatttactatcactgttggtaaccttttctgagctaaatatt420
tgaatgttatcggaatggtcagggtggtacaattttacataacgatgatatttttttttt480
aacgacttcttgtccagtttaggatttccagatccggcctttggaatgccaaaaatatca540
tagggagttggatctgccaactcaggccattgttcatcccttatcgtaagttttctattg600
ccatttttatcgttcgctgtagcatacttagctataaaagtgatttgtgggggacacttt660
tctacacatgataagtgccacttgaataaaaatgggtatacgaacttatggtgtagcata720
acaaatatattgcaagtagtgacctatggtgtgtagatatacgtacagttagttacgagc780
ctaaagacacaacgtgtttgttaattatactgtcgctgtaatatcttctcttccattatc840
accggtcattccttgcaggggcggtagtacccggagaccctgaacttttctttttttttt900
tgcgaaattaaaaagttcattttcaattcgacaatgagatctacaagccattgttttatg960
ttgatgagagccagcttaaagagttctcgagatctcccgagtttatcattatcaatactg1020
ccatttcaaagaatacgtaaataattaatagtagtgattttcctaactttatttagtcaa1080
aaaattggccttttaattctgctgtaacccgtacatgcccaaaatagggggcgggttaca1140
cagaatatataacatcataggtgtctgggtgaacagtttattcctggcatccactaaata1200
taatggagcccgctttttttaagctggcatccagaaaaaaaaagaatcccagcaccaaaa1260
tattgttttcttcaccaaccatcagttcataggtccattctcttagcgcaactacacaga1320
acaggggcacaaacaggcaaaaaacgggcacaacctcaatggagtgatgcaacctgcttg1380
gagtaaatgatgacacaaggcaattgacctacgcatgtatctatctcattttcttacacc1440
ttctattaccttctgctctctctgatttggaaaaagctgaaaaaaaaggttgaaaccagt1500
tccctgaaattattcccctatttgactaataagtatataaagacggtaggtattgattgt1560
aattctgtaaatctatttcttaaacttcttaaattctacttttatagttagtcttttttt1620
tagtttaaaacaccaagaacttagtttcgaataaacacacataaacaaacaaatctagaa1680
tgaaacttatgaatccatctatgaaggcatacgttttctttatcttaagctacttctctt1740
tactcgttagctcagctgcggtgccaacctctgccgccgtacaagttgagtcatacaatt1800
atgacggtaccactttttcaggtagaatattcgtcaaaaacattgcctactcaaaggtcg1860
taacagttatctactccgatggatcagataactggaacaataacaacaacaaagtttctg1920
cagcttactcagaagcaatttctgggtctaactacgaatactggacattctccgcaaagt1980
tatccggaattaaacagttttatgtcaaatacgaagtttctggttcaacatattacgaca2040
acaacggtaccaaaaactaccaagtccaagcaacctcagcgacatctacaacagctactg2100
caaccacaactacagctactggcacaacaactacttctacaggtccaactagtactgcat2160
ccgtatcattccctaccggtaactcaacaatttcttcctggataaaaaatcaagaggaaa2220
tcagccgttttgctatgttgagaaatatcaatccacctgggtctgccacagggttcatag2280
ccgcatctctgtccacagccggcccagattactattactcttggactagagattcagcac2340
taacagctaatgtgatcgcttacgaatacaacacaacattcactggaaacaccacccttc2400
ttaagtacttgaaagattacgttacattttctgtcaaaagccaatctgtatctaccgttt2460
gtaactgtctgggagaaccaaagttcaacgctgatggtagttcttttacaggtccatggg2520
gcagaccacaaaacgacggaccagcagagagagctgttacttttatgttgattgctgaca2580
gctacttgactcaaactaaggacgcatcctacgttaccggtacattaaagccagcaatct2640
tcaaagatcttgattacgtagtttctgtttggtctaacggttgctacgatttatgggaag2700
aggttaatggtgttcatttctatactctcatggtcatgagaaagggtttgatcttaggtg2760
ccgacttcgctgctagaaatggtgactctagtagagcttcaacctacaagcaaactgcat2820
caacaatggaatcaaagatcagttctttttggtcagattctaacaactacgtccaagttt2880
ctcaatcagttaccgccggagtgtcaaaaaagggactagatgttagtacactattggcgg2940
ccaacattggtagtctgcctgatggctttttcactccaggctccgaaaagatattggcta3000
cagcagtggcgttagaaaatgcattcgcatccttgtacccaattaactctaacctacctt3060
cttacttgggtaactcaattggaagatatcctgaggatacatacaacggtaatggcaact3120
ctcaggggaatccatggttccttgccgtcaacgcatacgcagaactttactacagagcta3180
ttaaggaatggattagtaatggcaaggtgacagtatccaatatctcactacctttcttca3240
aaaagtttgattcttccgccacttctggaaagacatacactgctggtacatcagatttca3300
ataacttggctcagaacattgctttaggcgccgatagattcctgtctactgttaagttcc3360
acgcatacactaacgggagtctatcagaagagtacgatagatctaccggtatgagtactg3420
gggctcgtgatttaacatggtcccatgcttcattgatcacagtggcgtacgcaaaggccg3480
gtagtcctgcagcttagttaattaaacaggccccttttcctttgtcgatatcatgtaatt3540
agttatgtcacgcttacattcacgccctcctcccacatccgctctaaccgaaaaggaagg3600
agttagacaacctgaagtctaggtccctatttatttttttatagttatgttagtattaag3660
aacgttatttatatttcaaatttttcttttttttctgtacaaacgcgtgtacgcatgtaa3720
cgggcagacggccggccataacttcgtataatgtatgctatacgaagttatggcaacggt3780
tcatcatctcatggatctgcacatgaacaaacaccagagtcaaacgacgttgaaattgag3840
gctactgcgccaattgatgacaatacagacgatgataacaaaccgaagttatctgatgta3900
gaaaaggattagagatgctaagagatagtgatgatatttcataaataatgtaattctata3960
tatgttaattaccttttttgcgaggcatatttatggtgaaggataagttttgaccatcaa4020
agaaggttaatgtggctgtggtttcagggtccataaagcttttcaattcatctttttttt4080
ttttgttcttttttttgattccggtttctttgaaatttttttgattcggtaatctccgag4140
cagaaggaagaacgaaggaaggagcacagacttagattggtatatatacgcatatgtggt4200
gttgaagaaacatgaaattgcccagtattcttaacccaactgcacagaacaaaaacctgc4260
aggaaacgaagataaatcatgtcgaaagctacatataaggaacgtgctgctactcatcct4320
agtcctgttgctgccaagctatttaatatcatgcacgaaaagcaaacaaacttgtgtgct4380
tcattggatgttcgtaccaccaaggaattactggagttagttgaagcattaggtcccaaa4440
atttgtttactaaaaacacatgtggatatcttgactgatttttccatggagggcacagtt4500
aagccgctaaaggcattatccgccaagtacaattttttactcttcgaagacagaaaattt4560
gctgacattggtaatacagtcaaattgcagtactctgcgggtgtatacagaatagcagaa4620
tgggcagacattacgaatgcacacggtgtggtgggcccaggtattgttagcggtttgaag4680
caggcggcggaagaagtaacaaaggaacctagaggccttttgatgttagcagaattgtca4740
tgcaagggctccctagctactggagaatatactaagggtactgttgacattgcgaagagc4800
gacaaagattttgttatcggctttattgctcaaagagacatgggtggaagagatgaaggt4860
tacgattggttgattatgacacgc4884
<210>94
<211>1790
<212>dna
<213>酿酒酵母(saccharomycescerevisiae)
<400>94
ggaagagctcctactgcgccaattgatgacaatacagacgatgataacaaaccgaagtta60
tctgatgtagaaaaggattagagatgctaagagatagtgatgatatttcataaataatgt120
aattctatatatgttaattaccttttttgcgaggcatatttatggtgaaggataagtttt180
gaccatcaaagaaggttaatgtggctgtggtttcagggtccataaagcttttcaattcat240
ctttttttttttgttcttttttttgattccggtttctttgaaatttttttgattcggtaa300
tctccgagcagaaggaagaacgaaggaaggagcacagacttagattggtatatatacgca360
tatgtggtgttgaagaaacatgaaattgcccagtattcttaacccaactgcacagaacaa420
aaacctgcaggaaacgaagataaatcatgtcgaaagctacatataaggaacgtgctgcta480
ctcatcctagtcctgttgctgccaagctatttaatatcatgcacgaaaagcaaacaaact540
tgtgtgcttcattggatgttcgtaccaccaaggaattactggagttagttgaagcattag600
gtcccaaaatttgtttactaaaaacacatgtggatatcttgactgatttttccatggagg660
gcacagttaagccgctaaaggcattatccgccaagtacaattttttactcttcgaagaca720
gaaaatttgctgacattggtaatacagtcaaattgcagtactctgcgggtgtatacagaa780
tagcagaatgggcagacattacgaatgcgcacggtgtggtgggcccaggtattgttagcg840
gtttgaagcaggcggcggaagaagtaacaaaggaacctagaggccttttgatgttagcag900
aattgtcatgcaagggctccctagctactggagaatatactaagggtactgttgacattg960
cgaagagcgacaaagattttgttatcggctttattgctcaaagagacatgggtggaagag1020
atgaaggttacgattggttgattatgacacccggtgtgggtttagatgacaagggagacg1080
cattgggtcaacagtatagagccgtggatgatgtggtctctacaggatctgacattatta1140
ttgttggaagaggactatttgcaaagggaagggatgctaaggtagagggtgaacgttaca1200
gaaaagcaggctgggaagcatatttgagaagatgcggccagcaaaactaaaaaactgtat1260
tataagtaaatgcatgtatactaaactcacaaattagagcttcaatttaattatatcagt1320
tattacccgggaatctcggtcgtaatgatttttataatgacgaaaaaaaaaaattggaaa1380
gaaaaagcttcatggcctttataaaaaggaaccatccaatacctcgccagaaccaagtaa1440
cagtattttacggggcacaaatcaagaacaataagacaggactgtaaagatggacgcatt1500
gaactccaaagaacaacaagagttccaaaaagtagtggaacaaaagcaaatgaaggattt1560
catgcgtttgataacttcgtataatgtatgctatacgaagttatgcggccgccagcacgc1620
agcacgctgtatttacgtatttaattttatatatttgtgcatacactactagggaagact1680
tgaaaaaaacctaggaaatgaaaaaacgacacaggaagtcccgtatttactattttttcc1740
ttccttttgatggggcagggcggaaatagaggataggataagcctactgc1790
<210>95
<211>605
<212>prt
<213>小孢根霉(rhizopusmicrosporus)
<400>95
metlysleumetasnprosermetlysalatyrvalphepheileleu
151015
sertyrpheserleuleuvalserseralaalavalprothrserala
202530
alavalglnvalglusertyrasntyraspglythrthrphesergly
354045
argilephevallysasnilealatyrserlysvalvalthrvalile
505560
tyrseraspglyseraspasntrpasnasnasnasnasnlysvalser
65707580
alaalatyrserglualaileserglyserasntyrglutyrtrpthr
859095
pheseralalysleuserglyilelysglnphetyrvallystyrglu
100105110
valserglyserthrtyrtyraspasnasnglythrlysasntyrgln
115120125
valglnalathrseralathrserthrthralathralathrthrthr
130135140
thralathrglythrthrthrthrserthrglyprothrserthrala
145150155160
servalserpheprothrglyasnserthrilesersertrpilelys
165170175
asnglnglugluileserargphealametleuargasnileasnpro
180185190
proglyseralathrglypheilealaalaserleuserthralagly
195200205
proasptyrtyrtyrsertrpthrargaspseralaleuthralaasn
210215220
valilealatyrglutyrasnthrthrphethrglyasnthrthrleu
225230235240
leulystyrleulysasptyrvalthrpheservallysserglnser
245250255
valserthrvalcysasncysleuglygluprolyspheasnalaasp
260265270
glyserserphethrglyprotrpglyargproglnasnaspglypro
275280285
alagluargalavalthrphemetleuilealaaspsertyrleuthr
290295300
glnthrlysaspalasertyrvalthrglythrleulysproalaile
305310315320
phelysaspleuasptyrvalvalservaltrpserasnglycystyr
325330335
aspleutrpglugluvalasnglyvalhisphetyrthrleumetval
340345350
metarglysglyleuileleuglyalaaspphealaalaargasngly
355360365
aspserserargalaserthrtyrlysglnthralaserthrmetglu
370375380
serlysileserserphetrpseraspserasnasntyrvalglnval
385390395400
serglnservalthralaglyvalserlyslysglyleuaspvalser
405410415
thrleuleualaalaasnileglyserleuproaspglyphephethr
420425430
proglyserglulysileleualathralavalalaleugluasnala
435440445
phealaserleutyrproileasnserasnleuprosertyrleugly
450455460
asnserileglyargtyrprogluaspthrtyrasnglyasnglyasn
465470475480
serglnglyasnprotrppheleualavalasnalatyralagluleu
485490495
tyrtyrargalailelysglutrpileserasnglylysvalthrval
500505510
serasnileserleuprophephelyslyspheaspserseralathr
515520525
serglylysthrtyrthralaglythrserasppheasnasnleuala
530535540
glnasnilealaleuglyalaaspargpheleuserthrvallysphe
545550555560
hisalatyrthrasnglyserleusergluglutyraspargserthr
565570575
glymetserthrglyalaargaspleuthrtrpserhisalaserleu
580585590
ilethrvalalatyralalysalaglyserproalaala
595600605
<210>96
<211>4474
<212>dna
<213>人工序列(artificialsequence)
<220>
<223>由多种生物体酿酒酵母、小孢根霉和构巢曲霉共同组成的序列:
<400>96
gtgtttgttaattatactgtcgctgtaatatcttctcttccattatcaccggtcattcct60
tgcaggggcggtagtacccggagaccctgaacttttctttttttttttgcgaaattaaaa120
agttcattttcaattcgacaatgagatctacaagccattgttttatgttgatgagagcca180
gcttaaagagttctcgagatctcccgagtttatcattatcaatactgccatttcaaagaa240
tacgtaaataattaatagtagtgattttcctaactttatttagtcaaaaaattggccttt300
taattctgctgtaacccgtacatgcccaaaatagggggcgggttacacagaatatataac360
atcataggtgtctgggtgaacagtttattcctggcatccactaaatataatggagcccgc420
tttttttaagctggcatccagaaaaaaaaagaatcccagcaccaaaatattgttttcttc480
accaaccatcagttcataggtccattctcttagcgcaactacacagaacaggggcacaaa540
caggcaaaaaacgggcacaacctcaatggagtgatgcaacctgcttggagtaaatgatga600
cacaaggcaattgacctacgcatgtatctatctcattttcttacaccttctattaccttc660
tgctctctctgatttggaaaaagctgaaaaaaaaggttgaaaccagttccctgaaattat720
tcccctatttgactaataagtatataaagacggtaggtattgattgtaattctgtaaatc780
tatttcttaaacttcttaaattctacttttatagttagtcttttttttagtttaaaacac840
caagaacttagtttcgaataaacacacataaacaaacaaatctagaatgaaacttatgaa900
tccatctatgaaggcatacgttttctttatcttaagctacttctctttactcgttagctc960
agctgcggtgccaacctctgccgccgtacaagttgagtcatacaattatgacggtaccac1020
tttttcaggtagaatattcgtcaaaaacattgcctactcaaaggtcgtaacagttatcta1080
ctccgatggatcagataactggaacaataacaacaacaaagtttctgcagcttactcaga1140
agcaatttctgggtctaactacgaatactggacattctccgcaaagttatccggaattaa1200
acagttttatgtcaaatacgaagtttctggttcaacatattacgacaacaacggtaccaa1260
aaactaccaagtccaagcaacctcagcgacatctacaacagctactgcaaccacaactac1320
agctactggcacaacaactacttctacaggtccaactagtactgcatccgtatcattccc1380
taccggtaactcaacaatttcttcctggataaaaaatcaagaggaaatcagccgttttgc1440
tatgttgagaaatatcaatccacctgggtctgccacagggttcatagccgcatctctgtc1500
cacagccggcccagattactattactcttggactagagattcagcactaacagctaatgt1560
gatcgcttacgaatacaacacaacattcactggaaacaccacccttcttaagtacttgaa1620
agattacgttacattttctgtcaaaagccaatctgtatctaccgtttgtaactgtctggg1680
agaaccaaagttcaacgctgatggtagttcttttacaggtccatggggcagaccacaaaa1740
cgacggaccagcagagagagctgttacttttatgttgattgctgacagctacttgactca1800
aactaaggacgcatcctacgttaccggtacattaaagccagcaatcttcaaagatcttga1860
ttacgtagtttctgtttggtctaacggttgctacgatttatgggaagaggttaatggtgt1920
tcatttctatactctcatggtcatgagaaagggtttgatcttaggtgccgacttcgctgc1980
tagaaatggtgactctagtagagcttcaacctacaagcaaactgcatcaacaatggaatc2040
aaagatcagttctttttggtcagattctaacaactacgtccaagtttctcaatcagttac2100
cgccggagtgtcaaaaaagggactagatgttagtacactattggcggccaacattggtag2160
tctgcctgatggctttttcactccaggctccgaaaagatattggctacagcagtggcgtt2220
agaaaatgcattcgcatccttgtacccaattaactctaacctaccttcttacttgggtaa2280
ctcaattggaagatatcctgaggatacatacaacggtaatggcaactctcaggggaatcc2340
atggttccttgccgtcaacgcatacgcagaactttactacagagctattaaggaatggat2400
tagtaatggcaaggtgacagtatccaatatctcactacctttcttcaaaaagtttgattc2460
ttccgccacttctggaaagacatacactgctggtacatcagatttcaataacttggctca2520
gaacattgctttaggcgccgatagattcctgtctactgttaagttccacgcatacactaa2580
cgggagtctatcagaagagtacgatagatctaccggtatgagtactggggctcgtgattt2640
aacatggtcccatgcttcattgatcacagtggcgtacgcaaaggccggtagtcctgcagc2700
ttagttaattaaacaggccccttttcctttgtcgatatcatgtaattagttatgtcacgc2760
ttacattcacgccctcctcccacatccgctctaaccgaaaaggaaggagttagacaacct2820
gaagtctaggtccctatttatttttttatagttatgttagtattaagaacgttatttata2880
tttcaaatttttcttttttttctgtacaaacgcgtgtacgcatgtaacgggcagacggcc2940
ggccataacttcgtataatgtatgctatacgaagttatccttacatcacacccaatcccc3000
cacaagtgatcccccacacaccatagcttcaaaatgtttctactccttttttactcttcc3060
agattttctcggactccgcgcatcgccgtaccacttcaaaacacccaagcacagcatact3120
aaatttcccctctttcttcctctagggtggcgttaattacccgtactaaaggtttggaaa3180
agaaaaaagagaccgcctcgtttctttttcttcgtcgaaaaaggcaataaaaatttttat3240
cacgtttctttttcttgaaaaattttttttttgatttttttctctttcgatgacctccca3300
ttgatatttaagttaataaatggtcttcaatttctcaagtttcagtttcgtttttcttgt3360
tctattacaactttttttacttcttgctcattagaaagaaagcatagcaatctaatctaa3420
gttttaattacaaaatgccacaatcctgggaagaattggccgccgacaaacgtgcccgtt3480
tggctaaaaccattcctgacgaatggaaggttcaaactttgcctgccgaagattccgtta3540
ttgatttcccaaagaagtccggtattttgtctgaggctgaattgaagattaccgaagcct3600
ctgctgctgatttggtctccaagttggccgctggtgagttgacttctgttgaagtcactt3660
tggctttttgtaagagagctgctattgctcaacaattaaccaactgtgctcacgaattct3720
tcccagatgctgctttagctcaagctagagaattagatgaatactacgctaagcataaga3780
gaccagttggtccattacacggtttaccaatctctttaaaggaccaattgcgtgttaagg3840
gttacgaaacctccatgggttacatttcctggttaaacaaatacgatgaaggtgattccg3900
tcttaaccaccatgttgagaaaagctggtgctgttttctacgttaagacctctgtcccac3960
aaaccttgatggtctgtgaaaccgtcaacaacatcattggtagaactgtcaatccaagaa4020
acaaaaattggtcctgtggtggttcttctggtggtgaaggtgctattgttggtattagag4080
gtggtgttattggtgtcggtactgacattggtggttccattagagtcccagctgctttca4140
actttttatacggtttgagaccatctcacggtagattgccatatgctaaaatggctaact4200
ctatggaaggtcaagaaaccgttcactccgtcgttggtcctatcactcactccgtcgaag4260
acttgagattgttcaccaaatctgtcttgggtcaagaaccttggaagtacgactctaagg4320
tcatccccatgccatggagacaatctgaatctgacatcattgcctctaagattaagaatg4380
gtggtttgaacattggttattacaatttcgacggtaacgtcttgccacacccaccaattt4440
tacgtggtgtcgaaactaccgttgccgctttggc4474
<210>97
<211>1878
<212>dna
<213>人工序列(artificialsequence)
<220>
<223>由多种生物体酿酒酵母和构巢曲霉共同组成的序列:
<400>97
gaagattaccgaagcctctgctgctgatttggtctccaagttggccgctggtgagttgac60
ttctgttgaagtcactttggctttttgtaagagagctgctattgctcaacaattaaccaa120
ctgtgctcacgaattcttcccagatgctgctttagctcaagctagagaattagatgaata180
ctacgctaagcataagagaccagttggtccattacacggtttaccaatctctttaaagga240
ccaattgcgtgttaagggttacgaaacctccatgggttacatttcctggttaaacaaata300
cgatgaaggtgattccgtcttaaccaccatgttgagaaaagctggtgctgttttctacgt360
taagacctctgtcccacaaaccttgatggtctgtgaaaccgtcaacaacatcattggtag420
aactgtcaatccaagaaacaaaaattggtcctgtggtggttcttctggtggtgaaggtgc480
tattgttggtattagaggtggtgttattggtgtcggtactgacattggtggttccattag540
agtcccagctgctttcaactttttatacggtttgagaccatctcacggtagattgccata600
tgctaaaatggctaactctatggaaggtcaagaaaccgttcactccgtcgttggtcctat660
cactcactccgtcgaagacttgagattgttcaccaaatctgtcttgggtcaagaaccttg720
gaagtacgactctaaggtcatcccaatgccatggagacaatctgaatctgacatcattgc780
ctctaagattaagaatggtggtttgaacattggttattacaatttcgacggtaacgtctt840
gccacacccaccaattttacgtggtgtcgaaactaccgttgccgctttggccaaggctgg900
tcacaccgttactccatggactccatacaagcatgatttcggtcatgacttgatttccca960
catctatgctgctgatggttctgccgacgtcatgagagacatttctgcctctggtgagcc1020
agccatccctaacattaaggacttgttgaacccaaatattaaggctgttaacatgaacga1080
attgtgggacactcatttacaaaagtggaactatcaaatggaatacttggaaaagtggcg1140
tgaagctgaagaaaaagctggtaaggaattggacgctattatcgctccaattactcctac1200
cgccgctgtcagacacgatcaattcagatactacggttacgcctccgttattaacttatt1260
ggatttcacctctgttgtcgtcccagtcactttcgctgataagaatattgataagaagaa1320
cgaatcttttaaagctgtttccgaattggatgctttggttcaagaagaatacgacccaga1380
ggcttatcacggtgctcctgttgctgttcaagttattggtagaagattgtccgaagagag1440
aactttggctatcgccgaagaagtcggtaaattgttgggtaacgtcgtcactccataagg1500
agattgataagacttttctagttgcatatcttttatatttaaatcttatctattagttaa1560
ttttttgtaatttatccttatatatagtctggttattctaaaatatcatttcagtatcta1620
aaaattcccctcttttttcagttatatcttaacaggcgataacttcgtataatgtatgct1680
atacgaagttatgcggccgccagcacgcagcacgctgtatttacgtatttaattttatat1740
atttgtgcatacactactagggaagacttgaaaaaaacctaggaaatgaaaaaacgacac1800
aggaagtcccgtatttactattttttccttccttttgatggggcagggcggaaatagagg1860
ataggataagcctactgc1878
<210>98
<211>8719
<212>dna
<213>人工序列(artificialsequence)
<220>
<223>由多种生物体酿酒酵母和p1噬菌体共同组成的序列:
<400>98
atcacataggaagcaacaggcgcgttggacttttaattttcgaggaccgcgaatccttac60
atcacacccaatcccccacaagtgatcccccacacaccatagcttcaaaatgtttctact120
ccttttttactcttccagattttctcggactccgcgcatcgccgtaccacttcaaaacac180
ccaagcacagcatactaaatttcccctctttcttcctctagggtgtcgttaattacccgt240
actaaaggtttggaaaagaaaaaagagaccgcctcgtttctttttcttcgtcgaaaaagg300
caataaaaatttttatcacgtttctttttcttgaaaatttttttttttgatttttttctc360
tttcgatgacctcccattgatatttaagttaataaacggtcttcaatttctcaagtttca420
gtttcatttttcttgttctattacaactttttttacttcttgctcattagaaagaaagca480
tagcaatctaatctaagttttaattacaaatctagaatgagtgaatctccaatgttcgct540
gccaacggcatgccaaaggtaaatcaaggtgctgaagaagatgtcagaattttaggttac600
gacccattagcttctccagctctccttcaagtgcaaatcccagccacaccaacttctttg660
gaaactgccaagagaggtagaagagaagctatagatattattaccggtaaagacgacaga720
gttcttgtcattgtcggtccttgttccatccatgatcttgaagccgctcaagaatacgct780
ttgagattaaagaaattgtcagatgaattaaaaggtgatttatccatcattatgagagca840
tacttggagaagccaagaacaaccgtcggctggaaaggtctaattaatgaccctgatgtt900
aacaacactttcaacatcaacaagggtttgcaatccgctagacaattgtttgtcaacttg960
acaaatatcggtttgccaattggttctgaaatgcttgataccatttctcctaaatacttg1020
gctgatttggtctccttcggtgccattggtgccagaaccaccgaatctcaactgcacaga1080
gaattggcctccggtttgtctttcccagttggtttcaagaacggtaccgatggtacctta1140
aatgttgctgtggatgcttgtcaagccgctgctcattctcaccatttcatgggtgttact1200
aagcatggtgttgctgctatcaccactactaagggtaacgaacactgcttcgttattcta1260
agaggtggtaaaaagggtaccaactacgacgctaagtccgttgcagaagctaaggctcaa1320
ttgcctgccggttccaacggtctaatgattgactactctcacggtaactccaataaggat1380
ttcagaaaccaaccaaaggtcaatgacgttgtttgtgagcaaatcgctaacggtgaaaac1440
gccattaccggtgtcatgattgaatcaaacatcaacgaaggtaaccaaggcatcccagcc1500
gaaggtaaagccggcttgaaatatggtgtttccatcactgatgcttgtataggttgggaa1560
actactgaagacgtcttgaggaaattggctgctgctgtcagacaaagaagagaagttaac1620
aagaaatagatgtttttttaatgatatatgtaacgtacattctttcctctaccactgcca1680
attcggtattatttaattgtgtttagcgctatttactaattaactagaaactcaattttt1740
aaaggcaaagctcgctgacctttcactgatttcgtggatgttatactatcagttactctt1800
ctgcaaaaaaaaattgagtcatatcgtagctttgggattatttttctctctctccacggc1860
taattaggtgatcatgaaaaaatgaaaaattcatgagaaaagagtcagacatcgaaacat1920
acataagttgatattcctttgatatcgacgactactcaatcaggttttaaaagaaaagag1980
gcagctattgaagtagcagtatccagtttaggttttttaattatttacaagtaaagaaaa2040
agagaatgccggtcgttcacggcggccgcgccagaaaaaggaagtgtttccctccttctt2100
gaattgatgttaccctcataaagcacgtggcctcttatcgagaaagaaattaccgtcgct2160
cgtgatttgtttgcaaaaagaacaaaactgaaaaaacccagacacgctcgacttcctgtc2220
ttcctattgattgcagcttccaatttcgtcacacaacaaggtcctagcgacggctcacag2280
gttttgtaacaagcaatcgaaggttctggaatggcgggaaagggtttagtaccacatgct2340
atgatgcccactgtgatctccagagcaaagttcgttcgatcgtactgttactctctctct2400
ttcaaacagaattgtccgaatcgtgtgacaacaacagcctgttctcacacactcttttct2460
tctaaccaagggggtggtttagtttagtagaacctcgtgaaacttacatttacatatata2520
taaacttgcataaattggtcaatgcaagaaatacatatttggtcttttctaattcgtagt2580
ttttcaagttcttagatgctttctttttctcttttttacagatcatcaactcttttttac2640
agatcatcaaggaagtaattatctactttttacaagaattcatgtctaatttacttactg2700
ttcaccaaaacttgcctgcattaccagttgacgcaacctccgatgaagtcagaaagaacc2760
ttatggatatgtttagagatagacaagctttctccgaacatacttggaaaatgttattat2820
ccgtttgtagatcctgggccgcttggtgtaaacttaacaatagaaaatggtttcctgctg2880
aaccagaagacgtcagagattacttactttacttacaagctagaggtttggctgttaaaa2940
ctatccaacaacacttaggtcaattgaatatgttacacagaagatccggtttaccaagac3000
catccgattccaacgcagtttcccttgttatgagaagaattagaaaagaaaatgttgacg3060
ctggtgaaagagctaaacaagcattagcatttgaaagaaccgatttcgatcaagttagat3120
ccttaatggaaaattccgatagatgtcaagatattagaaacttagctttcttaggtattg3180
cttacaacacattattaagaatcgctgaaattgctagaattagagttaaagatatttcaa3240
gaaccgatggcggtagaatgttaatccacattggcagaacaaaaaccttagtctccacag3300
caggcgtcgaaaaagcattatcattaggtgttactaaattagttgaacgttggatttccg3360
tttccggtgttgcagatgacccaaacaactacttattctgtcgtgttagaaaaaatggtg3420
ttgccgctccttccgctacctcacaattatccacaagagcattagaaggcatttttgaag3480
ctacccacagacttatttatggtgcaaaagacgattccggtcaaagatatttagcttggt3540
ctggtcattccgctagagttggtgccgcaagagacatggcaagagctggtgtttctattc3600
ctgaaattatgcaagccggtggttggactaatgttaacattgttatgaactatatcagaa3660
acttagattccgaaacaggtgctatggttagattacttgaagacggtgattaagctagct3720
aagatccgctctaaccgaaaaggaaggagttagacaacctgaagtctaggtccctattta3780
tttttttatagttatgttagtattaagaacgttatttatatttcaaatttttcttttttt3840
tctgtacagacgcgtgtacgcatgtaacattatactgaaaaccttgcttgagaaggtttt3900
gggacgctcgaaggagctccaattcgccctatagtgagtcgtattacaattcactggccg3960
tcgttttacaacgtcgtgactgggaaaaccctggcgttacccaacttaatcgccttgcag4020
cacatccccccttcgccagctggcgtaatagcgaagaggcccgcaccgatcgcccttccc4080
aacagttgcgcagcctgaatggcgaatggcgcgacgcgccctgtagcggcgcattaagcg4140
cggcgggtgtggtggttacgcgcagcgtgaccgctacacttgccagcgccctagcgcccg4200
ctcctttcgctttcttcccttcctttctcgccacgttcgccggctttccccgtcaagctc4260
taaatcgggggctccctttagggttccgatttagtgctttacggcacctcgaccccaaaa4320
aacttgattagggtgatggttcacgtagtgggccatcgccctgatagacggtttttcgcc4380
ctttgacgttggagtccacgttctttaatagtggactcttgttccaaactggaacaacac4440
tcaaccctatctcggtctattcttttgatttataagggattttgccgatttcggcctatt4500
ggttaaaaaatgagctgatttaacaaaaatttaacgcgaattttaacaaaatattaacgt4560
ttacaatttcctgatgcggtattttctccttacgcatctgtgcggtatttcacaccgcag4620
ggtaataactgatataattaaattgaagctctaatttgtgagtttagtatacatgcattt4680
acttataatacagttttttagttttgctggccgcatcttctcaaatatgcttcccagcct4740
gcttttctgtaacgttcaccctctaccttagcatcccttccctttgcaaatagtcctctt4800
ccaacaataataatgtcagatcctgtagagaccacatcatccacggttctatactgttga4860
cccaatgcgtctcccttgtcatctaaacccacaccgggtgtcataatcaaccaatcgtaa4920
ccttcatctcttccacccatgtctctttgagcaataaagccgataacaaaatctttgtcg4980
ctcttcgcaatgtcaacagtacccttagtatattctccagtagatagggagcccttgcat5040
gacaattctgctaacatcaaaaggcctctaggttcctttgttacttcttctgccgcctgc5100
ttcaaaccgctaacaatacctgggcccaccacaccgtgtgcattcgtaatgtctgcccat5160
tctgctattctgtatacacccgcagagtactgcaatttgactgtattaccaatgtcagca5220
aattttctgtcttcgaagagtaaaaaattgtacttggcggataatgcctttagcggctta5280
actgtgccctccatggaaaaatcagtcaagatatccacatgtgtttttagtaaacaaatt5340
ttgggacctaatgcttcaactaactccagtaattccttggtggtacgaacatccaatgaa5400
gcacacaagtttgtttgcttttcgtgcatgatattaaatagcttggcagcaacaggacta5460
ggatgagtagcagcacgttccttatatgtagctttcgacatgatttatcttcgtttcctg5520
caggtttttgttctgtgcagttgggttaagaatactgggcaatttcatgtttcttcaaca5580
ctacatatgcgtatatataccaatctaagtctgtgctccttccttcgttcttccttctgt5640
tcggagattaccgaatcaaaaaaatttcaaagaaaccgaaatcaaaaaaaagaataaaaa5700
aaaaatgatgaattgaattgaaaagcgtggtgcactctcagtacaatctgctctgatgcc5760
gcatagttaagccagccccgacacccgccaacacccgctgacgcgccctgacgggcttgt5820
ctgctcccggcatccgcttacagacaagctgtgaccgtctccgggagctgcatgtgtcag5880
aggttttcaccgtcatcaccgaaacgcgcgagacgaaagggcctcgtgatacgcctattt5940
ttataggttaatgtcatgataataatggtttcttaggacggatcgcttgcctgtaactta6000
cacgcgcctcgtatcttttaatgatggaataatttgggaatttactctgtgtttatttat6060
ttttatgttttgtatttggattttagaaagtaaataaagaaggtagaagagttacggaat6120
gaagaaaaaaaaataaacaaaggtttaaaaaatttcaacaaaaagcgtactttacatata6180
tatttattagacaagaaaagcagattaaatagatatacattcgattaacgataagtaaaa6240
tgtaaaatcacaggattttcgtgtgtggtcttctacacagacaagatgaaacaattcggc6300
attaatacctgagagcaggaagagcaagataaaaggtagtatttgttggcgatcccccta6360
gagtcttttacatcttcggaaaacaaaaactattttttctttaatttctttttttacttt6420
ctatttttaatttatatatttatattaaaaaatttaaattataattatttttatagcacg6480
tgatgaaaaggacccaggtggcacttttcggggaaatgtgcgcggaacccctatttgttt6540
atttttctaaatacattcaaatatgtatccgctcatgagacaataaccctgataaatgct6600
tcaataatattgaaaaaggaagagtatgagtattcaacatttccgtgtcgcccttattcc6660
cttttttgcggcattttgccttcctgtttttgctcacccagaaacgctggtgaaagtaaa6720
agatgctgaagatcagttgggtgcacgagtgggttacatcgaactggatctcaacagcgg6780
taagatccttgagagttttcgccccgaagaacgttttccaatgatgagcacttttaaagt6840
tctgctatgtggcgcggtattatcccgtattgacgccgggcaagagcaactcggtcgccg6900
catacactattctcagaatgacttggttgagtactcaccagtcacagaaaagcatcttac6960
ggatggcatgacagtaagagaattatgcagtgctgccataaccatgagtgataacactgc7020
ggccaacttacttctgacaacgatcggaggaccgaaggagctaaccgctttttttcacaa7080
catgggggatcatgtaactcgccttgatcgttgggaaccggagctgaatgaagccatacc7140
aaacgacgagcgtgacaccacgatgcctgtagcaatggcaacaacgttgcgcaaactatt7200
aactggcgaactacttactctagcttcccggcaacaattaatagactggatggaggcgga7260
taaagttgcaggaccacttctgcgctcggcccttccggctggctggtttattgctgataa7320
atctggagccggtgagcgtgggtctcgcggtatcattgcagcactggggccagatggtaa7380
gccctcccgtatcgtagttatctacacgacgggcagtcaggcaactatggatgaacgaaa7440
tagacagatcgctgagataggtgcctcactgattaagcattggtaactgtcagaccaagt7500
ttactcatatatactttagattgatttaaaacttcatttttaatttaaaaggatctaggt7560
gaagatcctttttgataatctcatgaccaaaatcccttaacgtgagttttcgttccactg7620
agcgtcagaccccgtagaaaagatcaaaggatcttcttgagatcctttttttctgcgcgt7680
aatctgctgcttgcaaacaaaaaaaccaccgctaccagcggtggtttgtttgccggatca7740
agagctaccaactctttttccgaaggtaactggcttcagcagagcgcagataccaaatac7800
tgtccttctagtgtagccgtagttaggccaccacttcaagaactctgtagcaccgcctac7860
atacctcgctctgctaatcctgttaccagtggctgctgccagtggcgataagtcgtgtct7920
taccgggttggactcaagacgatagttaccggataaggcgcagcggtcgggctgaacggg7980
gggttcgtgcacacagcccagcttggagcgaacgacctacaccgaactgagatacctaca8040
gcgtgagcattgagaaagcgccacgcttcccgaagggagaaaggcggacaggtatccggt8100
aagcggcagggtcggaacaggagagcgcacgagggagcttccaggggggaacgcctggta8160
tctttatagtcctgtcgggtttcgccacctctgacttgagcgtcgatttttgtgatgctc8220
gtcaggggggccgagcctatggaaaaacgccagcaacgcggcctttttacggttcctggc8280
cttttgctggccttttgctcacatgttctttcctgcgttatcccctgattctgtggataa8340
ccgtattaccgcctttgagtgagctgataccgctcgccgcagccgaacgaccgagcgcag8400
cgagtcagtgagcgaggaagcggaagagcgcccaatacgcaaaccgcctctccccgcgcg8460
ttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtga8520
gcgcaacgcaattaatgtgagttacctcactcattaggcaccccaggctttacactttat8580
gcttccggctcctatgttgtgtggaattgtgagcggataacaatttcacacaggaaacag8640
ctatgaccatgattacgccaagctcggaattaaccctcactaaagggaacaaaagctggg8700
taccgggccccccctcgag8719
<210>99
<211>1632
<212>dna
<213>酿酒酵母(saccharomycescerevisiae)
<400>99
ggcaacggttcatcatctcatggatctgcacatgaacaaacaccagagtcaaacgacgtt60
gaaattgaggctactgcgccaattgatgacaatacagacgatgataacaaaccgaagtta120
tctgatgtagaaaaggattagagatgctaagagatagtgatgatatttcataaataatgt180
aattctatatatgttaattaccttttttgcgaggcatatttatggtgaaggataagtttt240
gaccatcaaagaaggttaatgtggctgtggtttcagggtccataaagcttttcaattcat300
cttttttttttttgttcttttttttgattccggtttctttgaaatttttttgattcggta360
atctccgagcagaaggaagaacgaaggaaggagcacagacttagattggtatatatacgc420
atatgtggtgttgaagaaacatgaaattgcccagtattcttaacccaactgcacagaaca480
aaaacctgcaggaaacgaagataaatcatgtcgaaagctacatataaggaacgtgctgct540
actcatcctagtcctgttgctgccaagctatttaatatcatgcacgaaaagcaaacaaac600
ttgtgtgcttcattggatgttcgtaccaccaaggaattactggagttagttgaagcatta660
ggtcccaaaatttgtttactaaaaacacatgtggatatcttgactgatttttccatggag720
ggcacagttaagccgctaaaggcattatccgccaagtacaattttttactcttcgaagac780
agaaaatttgctgacattggtaatacagtcaaattgcagtactctgcgggtgtatacaga840
atagcagaatgggcagacattacgaatgcacacggtgtggtgggcccaggtattgttagc900
ggtttgaagcaggcggcggaagaagtaacaaaggaacctagaggccttttgatgttagca960
gaattgtcatgcaagggctccctagctactggagaatatactaagggtactgttgacatt1020
gcgaagagcgacaaagattttgttatcggctttattgctcaaagagacatgggtggaaga1080
gatgaaggttacgattggttgattatgacacccggtgtgggtttagatgacaagggagac1140
gcattgggtcaacagtatagaaccgtggatgatgtggtctctacaggatctgacattatt1200
attgttggaagaggactatttgcaaagggaagggatgctaaggtagagggtgaacgttac1260
agaaaagcaggctgggaagcatatttgagaagatgcggccagcaaaactaaaaaactgta1320
ttataagtaaatgcatgtatactaaactcacaaattagagcttcaatttaattatatcag1380
ttattacccgggaatctcggtcgtaatgatttttataatgacgaaaaaaaaaaaattgga1440
aagaaaaagcttcatggcctttataaaaaggaaccatccaatacctcgccagaaccaagt1500
aacagtattttacggggcacaaatcaagaacaataagacaggactgtaaagatggacgca1560
ttgaactccaaagaacaacaagagttccaaaaagtagtggaacaaaagcaaatgaaggat1620
ttcatgcgtttg1632
<210>100
<211>4158
<212>dna
<213>人工序列(artificialsequence)
<220>
<223>由多种生物体酿酒酵母和蜡样芽孢杆菌共同组成的序列:
<400>100
tgagctccgggtgggaggaaggcgcggcaattagaatgtgtgggtgcggaagctcgccgc60
tcccatcaagagagtggaagacgtatggtctgggtgcgaagtaccaccacgtttcttttt120
catctcttaagtgggattcttacgaaacacgtcacagggtcaaaagaaagagaacaaaag180
caatattgtaattgtctcagtccacggcaatgacatggcatggccccgaaggcttttttt240
gtctgtcttccttgggtcttaccccgccacgcgttaatagtgagacaagcaggaaatccg300
tatcattttctcgcatacacgaacccgcgtgcgcctggtaaattgcaggattctcattgt360
ccggttttctttatgggaataatcatcatcaccattatcactgttactcttgcgatcatc420
atcattaacataatttttttaacgctgtttgatgatggtatgtgcttttattgttcctta480
ctcaccttttcctttgtgtcttttaattttgaccattttgaccattttgacctttgatga540
tgtgtgagttcctcttttctttttttcttttcttttttcctttttttttcttttcttact600
gtgttaatcactttctttcctttttgttcatattgtcgtcttgttcattttcgttcaatt660
gataatgtatataaatctttcgtaagtatctcttgattgccatttttttctttccaagtt720
tccttgttctcgaggccagaaaaaggaagtgtttccctccttcttgaattgatgttaccc780
tcataaagcacgtggcctcttatcgagaaagaaattaccgtcgctcgtgatttgtttgca840
aaaagaacaaaactgaaaaaacccagacacgctcgacttcctgtcttcctattgattgca900
gcttccaatttcgtcacacaacaaggtcctagcgacggctcacaggttttgtaacaagca960
atcgaaggttctggaatggcgggaaagggtttagtaccacatgctatgatgcccactgtg1020
atctccagagcaaagttcgttcgatcgtactgttactctctctctttcaaacagaattgt1080
ccgaatcgtgtgacaacaacagcctgttctcacacactcttttcttctaaccaagggggt1140
ggtttagtttagtagaacctcgtgaaacttacatttacatatatataaacttgcataaat1200
tggtcaatgcaagaaatacatatttggtcttttctaattcgtagtttttcaagttcttag1260
atgctttctttttctcttttttacagatcatcaaggaagtaattatctactttttacaag1320
tctagaatgacaacatcaaatacctacaaattctatctaaacggtgaatggagagaatct1380
tcctctggagaaactattgagataccatcaccatacttacatgaagtgatcggacaggtt1440
caagcaatcactagaggagaggttgacgaagcgattgctagcgctaaggaagcacagaaa1500
tcttgggctgaggcatctctacaagatagagctaagtacttgtacaaatgggcagatgaa1560
ttggtaaacatgcaagacgaaatcgccgatatcatcatgaaggaagtgggcaagggttac1620
aaagacgctaaaaaggaggttgttagaaccgccgatttcatcagatacaccattgaagag1680
gcactccatatgcacggtgaatccatgatgggcgattcatttcctggtggaacaaaatct1740
aagctagcaataatccaaagagcgcctctgggtgtagtcttagccatcgctccattcaat1800
taccctgtaaacctttctgctgcaaaattggcaccagccttaattatgggtaacgctgtg1860
atattcaagccagcaactcagggtgctatttccggcatcaaaatggttgaagctttgcat1920
aaggctggtttgccaaagggtttggttaacgttgccacaggtagaggtagcgtcataggc1980
gattatttggtcgaacacgaagggataaacatggtttccttcaccggtggcactaacact2040
ggtaagcatttagcaaaaaaggcctcaatgattccattagtcttggaacttggtggcaaa2100
gatccaggcatcgttcgtgaagatgcagacctacaagatgctgcgaatcatatcgtatct2160
ggtgcgttcagttactcagggcagagatgtacagccattaagagagtccttgttcatgaa2220
aatgttgctgatgaactggtatcattggttaaggaacaagtggcaaagctttctgtggga2280
tcaccagagcaagattcaacaattgttcctctgattgacgataagtccgctgattttgtt2340
cagggtttagtggacgatgcagtcgaaaagggcgctacaattgtcattgggaacaagaga2400
gaacgtaacctaatctacccaacattgattgatcacgtcacagaggaaatgaaagttgcc2460
tgggaggaaccattcggtcctattcttccaattattagagttagtagcgacgagcaagct2520
attgaaattgcaaataagagtgagttcggattacaagcttctgtgtttaccaaagacata2580
aacaaggcattcgcaatcgcaaataagattgagactggttcagtgcaaatcaacggtaga2640
acagagagaggaccagatcactttccttttatcggggttaagggatctgggatgggtgcc2700
caaggcatcagaaagtctttggaatctatgactagagaaaaagttactgtcttaaatctc2760
gtatgattaaacaggccccttttcctttgtcgatatcatgtaattagttatgtcacgctt2820
acattcacgccctcctcccacatccgctctaaccgaaaaggaaggagttagacaacctga2880
agtctaggtccctatttatttttttatagttatgttagtattaagaacgttatttatatt2940
tcaaatttttcttttttttctgtacaaacgcgtgtacgcatgtaacgggcagacggccgg3000
ccataacttcgtataatgtatgctatacgaagttatggcaacggttcatcatctcatgga3060
tctgcacatgaacaaacaccagagtcaaacgacgttgaaattgaggctactgcgccaatt3120
gatgacaatacagacgatgataacaaaccgaagttatctgatgtagaaaaggattagaga3180
tgctaagagatagtgatgatatttcataaataatgtaattctatatatgttaattacctt3240
ttttgcgaggcatatttatggtgaaggataagttttgaccatcaaagaaggttaatgtgg3300
ctgtggtttcagggtccataaagcttttcaattcatcttttttttttttgttcttttttt3360
tgattccggtttctttgaaatttttttgattcggtaatctccgagcagaaggaagaacga3420
aggaaggagcacagacttagattggtatatatacgcatatgtggtgttgaagaaacatga3480
aattgcccagtattcttaacccaactgcacagaacaaaaacctgcaggaaacgaagataa3540
atcatgtcgaaagctacatataaggaacgtgctgctactcatcctagtcctgttgctgcc3600
aagctatttaatatcatgcacgaaaagcaaacaaacttgtgtgcttcattggatgttcgt3660
accaccaaggaattactggagttagttgaagcattaggtcccaaaatttgtttactaaaa3720
acacatgtggatatcttgactgatttttccatggagggcacagttaagccgctaaaggca3780
ttatccgccaagtacaattttttactcttcgaagacagaaaatttgctgacattggtaat3840
acagtcaaattgcagtactctgcgggtgtatacagaatagcagaatgggcagacattacg3900
aatgcacacggtgtggtgggcccaggtattgttagcggtttgaagcaggcggcggaagaa3960
gtaacaaaggaacctagaggccttttgatgttagcagaattgtcatgcaagggctcccta4020
gctactggagaatatactaagggtactgttgacattgcgaagagcgacaaagattttgtt4080
atcggctttattgctcaaagagacatgggtggaagagatgaaggttacgattggttgatt4140
atgacacgcggccgcggc4158
<210>101
<211>1127
<212>dna
<213>酿酒酵母(saccharomycescerevisiae)
<400>101
gctccatggagggcacagttaagccgctaaaggcattatccgccaagtacaattttttac60
tcttcgaagacagaaaatttgctgacattggtaatacagtcaaattgcagtactctgcgg120
gtgtatacagaatagcagaatgggcagacattacgaatgcacacggtgtggtgggcccag180
gtattgttagcggtttgaagcaggcggcggaagaagtaacaaaggaacctagaggccttt240
tgatgttagcagaattgtcatgcaagggctccctagctactggagaatatactaagggta300
ctgttgacattgcgaagagcgacaaagattttgttatcggctttattgctcaaagagaca360
tgggtggaagagatgaaggttacgattggttgattatgacacccggtgtgggtttagatg420
acaagggagacgcattgggtcaacagtatagaaccgtggatgatgtggtctctacaggat480
ctgacattattattgttggaagaggactatttgcaaagggaagggatgctaaggtagagg540
gtgaacgttacagaaaagcaggctgggaagcatatttgagaagatgcggccagcaaaact600
aaaaaactgtattataagtaaatgcatgtatactaaactcacaaattagagcttcaattt660
aattatatcagttattacccgggaatctcggtcgtaatgatttttataatgacgaaaaaa720
aaaaaattggaaagaaaaagcttcatggcctttataaaaaggaaccatccaatacctcgc780
cagaaccaagtaacagtattttacggggcacaaatcaagaacaataagacaggactgtaa840
agatggacgcattgaactccaaagaacaacaagagttccaaaaagtagtggaacaaaagc900
aaatgaaggatttcatgcgtttgataacttcgtataatgtatgctatacgaagttatctc960
gaggataaaactactacgctaaaaataaaataaaaatgtatgatttccctccatttccga1020
ccaattgtataattttatatctgcatgacttaataatataatataatacttataaaatac1080
gaatagaaaaatttaaaccgatgtaatgcatccttttctttgttgtc1127
<210>102
<211>4542
<212>dna
<213>人工序列(artificialsequence)
<220>
<223>由多种生物体酿酒酵母、蜡样芽孢杆菌和构巢曲霉共同组成的序列:
<400>102
tgagctccgggtgggaggaaggcgcggcaattagaatgtgtgggtgcggaagctcgccgc60
tcccatcaagagagtggaagacgtatggtctgggtgcgaagtaccaccacgtttcttttt120
catctcttaagtgggattcttacgaaacacgtcacagggtcaaaagaaagagaacaaaag180
caatattgtaattgtctcagtccacggcaatgacatggcatggccccgaaggcttttttt240
gtctgtcttccttgggtcttaccccgccacgcgttaatagtgagacaagcaggaaatccg300
tatcattttctcgcatacacgaacccgcgtgcgcctggtaaattgcaggattctcattgt360
ccggttttctttatgggaataatcatcatcaccattatcactgttactcttgcgatcatc420
atcattaacataatttttttaacgctgtttgatgatggtatgtgcttttattgttcctta480
ctcaccttttcctttgtgtcttttaattttgaccattttgaccattttgacctttgatga540
tgtgtgagttcctcttttctttttttcttttcttttttcctttttttttcttttcttact600
gtgttaatcactttctttcctttttgttcatattgtcgtcttgttcattttcgttcaatt660
gataatgtatataaatctttcgtaagtatctcttgattgccatttttttctttccaagtt720
tccttgttctcgaggccagaaaaaggaagtgtttccctccttcttgaattgatgttaccc780
tcataaagcacgtggcctcttatcgagaaagaaattaccgtcgctcgtgatttgtttgca840
aaaagaacaaaactgaaaaaacccagacacgctcgacttcctgtcttcctattgattgca900
gcttccaatttcgtcacacaacaaggtcctagcgacggctcacaggttttgtaacaagca960
atcgaaggttctggaatggcgggaaagggtttagtaccacatgctatgatgcccactgtg1020
atctccagagcaaagttcgttcgatcgtactgttactctctctctttcaaacagaattgt1080
ccgaatcgtgtgacaacaacagcctgttctcacacactcttttcttctaaccaagggggt1140
ggtttagtttagtagaacctcgtgaaacttacatttacatatatataaacttgcataaat1200
tggtcaatgcaagaaatacatatttggtcttttctaattcgtagtttttcaagttcttag1260
atgctttctttttctcttttttacagatcatcaaggaagtaattatctactttttacaag1320
tctagaatgacaacatcaaatacctacaaattctatctaaacggtgaatggagagaatct1380
tcctctggagaaactattgagataccatcaccatacttacatgaagtgatcggacaggtt1440
caagcaatcactagaggagaggttgacgaagcgattgctagcgctaaggaagcacagaaa1500
tcttgggctgaggcatctctacaagatagagctaagtacttgtacaaatgggcagatgaa1560
ttggtaaacatgcaagacgaaatcgccgatatcatcatgaaggaagtgggcaagggttac1620
aaagacgctaaaaaggaggttgttagaaccgccgatttcatcagatacaccattgaagag1680
gcactccatatgcacggtgaatccatgatgggcgattcatttcctggtggaacaaaatct1740
aagctagcaataatccaaagagcgcctctgggtgtagtcttagccatcgctccattcaat1800
taccctgtaaacctttctgctgcaaaattggcaccagccttaattatgggtaacgctgtg1860
atattcaagccagcaactcagggtgctatttccggcatcaaaatggttgaagctttgcat1920
aaggctggtttgccaaagggtttggttaacgttgccacaggtagaggtagcgtcataggc1980
gattatttggtcgaacacgaagggataaacatggtttccttcaccggtggcactaacact2040
ggtaagcatttagcaaaaaaggcctcaatgattccattagtcttggaacttggtggcaaa2100
gatccaggcatcgttcgtgaagatgcagacctacaagatgctgcgaatcatatcgtatct2160
ggtgcgttcagttactcagggcagagatgtacagccattaagagagtccttgttcatgaa2220
aatgttgctgatgaactggtatcattggttaaggaacaagtggcaaagctttctgtggga2280
tcaccagagcaagattcaacaattgttcctctgattgacgataagtccgctgattttgtt2340
cagggtttagtggacgatgcagtcgaaaagggcgctacaattgtcattgggaacaagaga2400
gaacgtaacctaatctacccaacattgattgatcacgtcacagaggaaatgaaagttgcc2460
tgggaggaaccattcggtcctattcttccaattattagagttagtagcgacgagcaagct2520
attgaaattgcaaataagagtgagttcggattacaagcttctgtgtttaccaaagacata2580
aacaaggcattcgcaatcgcaaataagattgagactggttcagtgcaaatcaacggtaga2640
acagagagaggaccagatcactttccttttatcggggttaagggatctgggatgggtgcc2700
caaggcatcagaaagtctttggaatctatgactagagaaaaagttactgtcttaaatctc2760
gtatgattaaacaggccccttttcctttgtcgatatcatgtaattagttatgtcacgctt2820
acattcacgccctcctcccacatccgctctaaccgaaaaggaaggagttagacaacctga2880
agtctaggtccctatttatttttttatagttatgttagtattaagaacgttatttatatt2940
tcaaatttttcttttttttctgtacaaacgcgtgtacgcatgtaacgggcagacggccgg3000
ccataacttcgtataatgtatgctatacgaagttatccttacatcacacccaatccccca3060
caagtgatcccccacacaccatagcttcaaaatgtttctactccttttttactcttccag3120
attttctcggactccgcgcatcgccgtaccacttcaaaacacccaagcacagcatactaa3180
atttcccctctttcttcctctagggtggcgttaattacccgtactaaaggtttggaaaag3240
aaaaaagagaccgcctcgtttctttttcttcgtcgaaaaaggcaataaaaatttttatca3300
cgtttctttttcttgaaaaattttttttttgatttttttctctttcgatgacctcccatt3360
gatatttaagttaataaatggtcttcaatttctcaagtttcagtttcgtttttcttgttc3420
tattacaactttttttacttcttgctcattagaaagaaagcatagcaatctaatctaagt3480
tttaattacaaaatgccacaatcctgggaagaattggccgccgacaaacgtgcccgtttg3540
gctaaaaccattcctgacgaatggaaggttcaaactttgcctgccgaagattccgttatt3600
gatttcccaaagaagtccggtattttgtctgaggctgaattgaagattaccgaagcctct3660
gctgctgatttggtctccaagttggccgctggtgagttgacttctgttgaagtcactttg3720
gctttttgtaagagagctgctattgctcaacaattaaccaactgtgctcacgaattcttc3780
ccagatgctgctttagctcaagctagagaattagatgaatactacgctaagcataagaga3840
ccagttggtccattacacggtttaccaatctctttaaaggaccaattgcgtgttaagggt3900
tacgaaacctccatgggttacatttcctggttaaacaaatacgatgaaggtgattccgtc3960
ttaaccaccatgttgagaaaagctggtgctgttttctacgttaagacctctgtcccacaa4020
accttgatggtctgtgaaaccgtcaacaacatcattggtagaactgtcaatccaagaaac4080
aaaaattggtcctgtggtggttcttctggtggtgaaggtgctattgttggtattagaggt4140
ggtgttattggtgtcggtactgacattggtggttccattagagtcccagctgctttcaac4200
tttttatacggtttgagaccatctcacggtagattgccatatgctaaaatggctaactct4260
atggaaggtcaagaaaccgttcactccgtcgttggtcctatcactcactccgtcgaagac4320
ttgagattgttcaccaaatctgtcttgggtcaagaaccttggaagtacgactctaaggtc4380
atccccatgccatggagacaatctgaatctgacatcattgcctctaagattaagaatggt4440
ggtttgaacattggttattacaatttcgacggtaacgtcttgccacacccaccaatttta4500
cgtggtgtcgaaactaccgttgccgctttggcggccgcggca4542
<210>103
<211>1363
<212>dna
<213>人工序列(artificialsequence)
<220>
<223>由多种生物体酿酒酵母和构巢曲霉共同组成的序列:
<400>103
agaggtggtgttattggtgtcggtactgacattggtggttccattagagtcccagctgct60
ttcaactttttatacggtttgagaccatctcacggtagattgccatatgctaaaatggct120
aactctatggaaggtcaagaaaccgttcactccgtcgttggtcctatcactcactccgtc180
gaagacttgagattgttcaccaaatctgtcttgggtcaagaaccttggaagtacgactct240
aaggtcatccccatgccatggagacaatctgaatctgacatcattgcctctaagattaag300
aatggtggtttgaacattggttattacaatttcgacggtaacgtcttgccacacccacca360
attttacgtggtgtcgaaactaccgttgccgctttggccaaggctggtcacaccgttact420
ccatggactccatacaagcatgatttctgtcatgacttgatttcccacatctatgctgct480
gatggttctgccgacgtcatgagagacatttctgcctctggtgagccagccatccctaac540
attaaggacttgttgaacccaaatattaaggctgttaacatgaacgaattgtgggacact600
catttacaaaagtggaactatcaaatggaatacttggaaaagtggcgtgaagctgaagaa660
aaagctggtaaggaattggacgctattatcgctccaattactcctaccgtcgctgtcaga720
cacgatcaattcagatactacggttacgcctccgttattagcttattggatttcacctct780
gttgtcgtcccagtcactttcgctgataagaatattgataagaagaacgaatcttttaaa840
gctgtttccgaattggatgctttggttcaagaagaatacgacccagaggcttatcacggt900
gctcctgttgctgttcaagttattggtagaagattgtccgaagagagaactttggctatc960
gccgaagaagtcggtaaattgttgggtaacgtcgtcactccataaggagattgataagac1020
ttttctagttgcatatcttttatatttaaatcttatctattagttaattttttgtaattt1080
atccttatatatagtctggttattctaaaatatcatttcagtatctaaaaattcccctct1140
tttttcagttatatcttaacaggcgataacttcgtataatgtatgctatacgaagttatg1200
ataaaactactacgctaaaaataaaataaaaatgtatgatttccctccatttccgaccaa1260
ttgtataattttatatctgcatgacttaataatataatataatacttataaaatacgaat1320
agaaaaatttaaaccgatgtaatgcatccttttctttgttgtc1363
<210>104
<211>5087
<212>dna
<213>人工序列(artificialsequence)
<220>
<223>由多种生物体酿酒酵母和稻瘟病菌共同组成的序列:
<400>104
ggccgctccatggagggcacagttaagccgctaaaggcattatccgccaagtacaatttt60
ttactcttcgaagacagaaaatttgctgacattggtaatacagtcaaattgcagtactct120
gcgggtgtatacagaatagcagaatgggcagacattacgaatgcacacggtgtggtgggc180
ccaggtattgttagcggtttgaagcaggcggcggaagaagtaacaaaggaacctagaggc240
cttttgatgttagcagaattgtcatgcaagggctccctagctactggagaatatactaag300
ggtactgttgacattgcgaagagcgacaaagattttgttatcggctttattgctcaaaga360
gacatgggtggaagagatgaaggttacgattggttgattatgacacccggtgtgggttta420
gatgacaagggagacgcattgggtcaacagtatagaaccgtggatgatgtggtctctaca480
ggatctgacattattattgttggaagaggactatttgcaaagggaagggatgctaaggta540
gagggtgaacgttacagaaaagcaggctgggaagcatatttgagaagatgcggccagcaa600
aactaaaaaactgtattataagtaaatgcatgtatactaaactcacaaattagagcttca660
atttaattatatcagttattacccgggaatctcggtcgtaatgatttttataatgacgaa720
aaaaaaaaaattggaaagaaaaagcttcatggcctttataaaaaggaaccatccaatacc780
tcgccagaaccaagtaacagtattttacggggcacaaatcaagaacaataagacaggact840
gtaaagatggacgcattgaactccaaagaacaacaagagttccaaaaagtagtggaacaa900
aagcaaatgaaggatttcatgcgtttgataacttcgtataatgtatgctatacgaagtta960
tctcgagtaacacttacagagtcctatcaggaaagaataaaagccgatcaagcttcattc1020
tcaggtaacaactggagcactggcgagctttgctctactatataagataaaatatgcact1080
aaaagtttgcatttctttacataactaaaactaagacattatgcatagcttacctgatca1140
aaaagtatgtaaacttgttaacatcttcacatgtgattcatctggtcgtactttcttgcg1200
gtgcagtgtaatatttctacccacgtgactataattgagcttgaaaactgtggcgttttt1260
ccaccgatgggtccacgccagatattaaccgaagccaaaataccgatgaaatttctgaga1320
tagctcttgtaaacgacgtcaaatcttcatatgcaaggagatcttgatttctttttggta1380
gtcatctgtcgtcttgaggcgtataagaaggaggttatatctgtcctttctacaaagtat1440
tttcgagaatcttgcctctgccccttttttctttttttaaaaggtttaaaaaacataact1500
gtcttcaatatatccagtatttacgacaatatacaaacataatctctagaatgaaattca1560
tctccaccttccttacattcatcctagcagccgtgagcgttacagccgtatacattaacg1620
gttctataatcacaccatgtgattctttaatttactgtagaggggaattgttaaaggaag1680
tcgaattagcacatccatttgccgatagtaagacattcgttgatatgccaactatcaaac1740
ctgtcgatgaagtgattgaagccttcaataagttacaaaagccactgtctaacaacacag1800
agctacaagattttctaagagagaacttcgcacaagccggcggagagttggaagaggttc1860
caaattctgaattagaaaccgatccagttttcttagacaaattggatgacaccgttatca1920
gagaatttgttgaaaaagttattgatatatggccttcactgacacgtagatacaagggtc1980
catctaattgtgaggcttgcgctgactcctttattccagttaatagaaccttcgtagttg2040
ctgggggcagatttagagaaccatattattgggacagttactggattcttgagggtttgc2100
tgagaactggtggcgctttcactaacatttctaaaaacaccgttgaaaacttcttagatt2160
tggtcgaaacaattggcttcgtaccaaacggtgcacgtatttactacaaaaatagatcac2220
aacctccattgttgtctcaaatggtgcgtatttacgtggaacatacaaatgacaccagta2280
tcctcggtagagctgtgcctttgttgatcaaggaacatgaatttttcattaacaaccgtt2340
caatcgatgttgaggcctccaacggtaaaacatacagattacagagatacgctgtaacca2400
atactcaaccaagacctgagtcttatagagaagattacataacagccagtaacagatcct2460
actactcaccatctggtatcatctaccctgaatcccaccaactaaacgaatctgaaaagg2520
ctgttctgtatagccatttagcatcaggggcagaatctggatgggactatacctcaagat2580
ggctctctacaccttctgatgccgtgagagataactactttccattgagatcactaaata2640
caaacaatatagttccagtagaccttaactcaattttgtacgcaaacgaagtggccatcg2700
ctgagttccttaacagaactggaaacagtacaggtgcatcagattggatggatctagcaa2760
agcaaagatcagaggctatgtacgcccttatgtggaatgaaactctctggtcatactttg2820
attacaacatgacatctaagactcaaaacagattcataccagtcgatgaagatgcagttt2880
caattgagacaaataacgctccagcaggacaacaagtctttttccatgtcgcacaatact2940
accctttctggactggcgcagccccaagatccttgaaaaataatccattggctgttttga3000
gagcatacgagcgtatcgatgcttacttggacatcaagcgtggagcaattccagcgacaa3060
acttaaagactggtcagcaatgggacgagccaaacgtctggcctccattaatgcacattc3120
tgatggaagggctgaccagagtaccagcgacatttggggaagatgatgtcgcatggacag3180
aaatacaagacttagctctaagacttggtcagagatacttagattctactttttgcactt3240
ggtacgctactggtggttcaacatctgaaacaccacagctacagggattgaacgctgaag3300
ataagggtatcatgttcgaaaagtacggtgacaattctacaaacgttgctggttccggcg3360
gggagtacgaagtggttgagggtttcggttggacaaatggtgttttgatgtgggtcgcag3420
atactttcaataacaagctcactagacctgattgcggtaacattaccgctgcaaatgttc3480
actccgacggttctcaagccagaaagagaggtgagatgtggtcagctttagaaatgcatc3540
cttacgatgcagcttggactaaagagttcggtgcgagaaaggttagaagagacaaggccg3600
aagcgagagcacttggaaatgttatgggcggcgtctgattaattaatttaccagcttact3660
atccttcttgaaaatatgcactctatatcttttagttcttaattgcaacacatagatttg3720
ctgtataacgaattttatgctatttttttaatttggagttcggtgatgaaagtgtcacag3780
cgaatttcctcacatgtagggaccgaattgtttacaagttctctgtaccaccatggagac3840
atcaaagattgaaaatctatggaaagatatggacggtagcaacaagaatatagcacgagc3900
cgcggagttcatttcgttacttttgatatcgctcacaactattgcgaagcgcttcagtga3960
aaaaatcataaggaaaagttgtaaatattattggtagtattcgtttggtaaagtagaggg4020
ggtaatttttcccctttattttgttcatacattcttaaattgctttgcctctccttttgg4080
aaagctatacttcggagcactgttgagcgaaggctcaggccggcagcacgcagcacgctg4140
tatttacgtatttaattttatatatttgtgcatacactactagggaagacttgaaaaaaa4200
cctaggaaatgaaaaaacgacacaggaagtcccgtatttactattttttccttccttttg4260
atggggcagggcggaaatagaggataggataagcctactgcttagctgtttccgtctcta4320
cttcggtagttgtctcaattgtcgtttcagtattacctttagagccgctagacgatggtt4380
gagctatttgttgagggaaaactaagttcatgtaacacacgcataacccgattaaactca4440
tgaatagcttgattgcaggaggctggtccattggagatggtgccttattttccttatagg4500
caacgatgatgtcttcgtcggtgttcaggtagtagtgtacactctgaatcagggagaacc4560
aggcaatgaacttgttcctcaagaaaatagcggccataggcatggattggttaaccacac4620
cagatatgcttggtgtggcagaatatagtccttttggtggcgcaattttcttgtacctgt4680
ggtagaaagggagcggttgaactgttagtatatattggcaatatcagcaaatttgaaaga4740
aaattgtcggtgaaaaacatacgaaacacaaaggtcgggccttgcaacgttattcaaagt4800
cattgtttagttgaggaggtagcagcggagtatatgtattccttttttttgcctatggat4860
gttgtaccatgcccattctgctcaagcttttgttaaaattatttttcagtattttttctt4920
ccatgttgcgcgttacgagaacagaagcgacagataaccgcaatcatacaactagcgcta4980
ctgcggggtgtaaaaagcacaagaactaagccaagatcacaacagttatcgataaaatag5040
cagtgtttgcatggccattgagaaggacaacattggcgtgcggcatg5087
<210>105
<211>5236
<212>dna
<213>人工序列(artificialsequence)
<220>
<223>由多种生物体酿酒酵母、稻瘟病菌和构巢曲霉共同组成的序列:
<400>105
ggccgcgaaggtgctattgttggtattagaggtggtgttattggtgtcggtactgacatt60
ggtggttccattagagtcccagctgctttcaactttttatacggtttgagaccatctcac120
ggtagattgccatatgctaaaatggctaactctatggaaggtcaagaaaccgttcactcc180
gtcgttggtcctatcactcactccgtcgaagacttgagattgttcaccaaatctgtcttg240
ggtcaagaaccttggaagtacgactctaaggtcatcccaatgccatggagacaatctgaa300
tctgacatcattgcctctaagattaagaatggtggtttgaacattggttattacaatttc360
gacggtaacgtcttgccacacccaccaattttacgtggtgtcgaaactaccgttgccgct420
ttggccaaggctggtcacaccgttactccatggactccatacaagcatgatttcggtcat480
gacttgatttcccacatctatgctgctgatggttctgccgacgtcatgagagacatttct540
gcctctggtgagccagccatccctaacattaaggacttgttgaacccaaatattaaggct600
gttaacatgaacgaattgtgggacactcatttacaaaagtggaactatcaaatggaatac660
ttggaaaagtggcgtgaagctgaagaaaaagctggtaaggaattggacgctattatcgct720
ccaattactcctaccgccgctgtcagacacgatcaattcagatactacggttacgcctcc780
gttattaacttattggatttcacctctgttgtcgtcccagtcactttcgctgataagaat840
attgataagaagaacgaatcttttaaagctgtttccgaattggatgctttggttcaagaa900
gaatacgacccagaggcttatcacggtgctcctgttgctgttcaagttattggtagaaga960
ttgtccgaagagagaactttggctatcgccgaagaagtcggtaaattgttgggtaacgtc1020
gtcactccataagcgaatttcttatgatttatgatttttattattaaataagttataaaa1080
aaaataagtgtatacaaattttaaagtgactcttaggttttaaaacgaaaattcttattc1140
ttgagtaactctttcctgtaggtcaggttgctttctcaggtatagcatgaggtcgctctt1200
attgaccacacctctaccggcatgccgagcaaatgcctgcaaatcgctccccatttcacc1260
caattgtagatatgctaactccagcaatgagttgatgaatctcggtgtgtattttatgtc1320
ctcagaggacaacacataacttcgtataatgtatgctatacgaagttatctcgagtaaca1380
cttacagagtcctatcaggaaagaataaaagccgatcaagcttcattctcaggtaacaac1440
tggagcactggcgagctttgctctactatataagataaaatatgcactaaaagtttgcat1500
ttctttacataactaaaactaagacattatgcatagcttacctgatcaaaaagtatgtaa1560
acttgttaacatcttcacatgtgattcatctggtcgtactttcttgcggtgcagtgtaat1620
atttctacccacgtgactataattgagcttgaaaactgtggcgtttttccaccgatgggt1680
ccacgccagatattaaccgaagccaaaataccgatgaaatttctgagatagctcttgtaa1740
acgacgtcaaatcttcatatgcaaggagatcttgatttctttttggtagtcatctgtcgt1800
cttgaggcgtataagaaggaggttatatctgtcctttctacaaagtattttcgagaatct1860
tgcctctgccccttttttctttttttaaaaggtttaaaaaacataactgtcttcaatata1920
tccagtatttacgacaatatacaaacataatctctagaatgaaattcatctccaccttcc1980
ttacattcatcctagcagccgtgagcgttacagccgtatacattaacggttctataatca2040
caccatgtgattctttaatttactgtagaggggaattgttaaaggaagtcgaattagcac2100
atccatttgccgatagtaagacattcgttgatatgccaactatcaaacctgtcgatgaag2160
tgattgaagccttcaataagttacaaaagccactgtctaacaacacagagctacaagatt2220
ttctaagagagaacttcgcacaagccggcggagagttggaagaggttccaaattctgaat2280
tagaaaccgatccagttttcttagacaaattggatgacaccgttatcagagaatttgttg2340
aaaaagttattgatatatggccttcactgacacgtagatacaagggtccatctaattgtg2400
aggcttgcgctgactcctttattccagttaatagaaccttcgtagttgctgggggcagat2460
ttagagaaccatattattgggacagttactggattcttgagggtttgctgagaactggtg2520
gcgctttcactaacatttctaaaaacaccgttgaaaacttcttagatttggtcgaaacaa2580
ttggcttcgtaccaaacggtgcacgtatttactacaaaaatagatcacaacctccattgt2640
tgtctcaaatggtgcgtatttacgtggaacatacaaatgacaccagtatcctcggtagag2700
ctgtgcctttgttgatcaaggaacatgaatttttcattaacaaccgttcaatcgatgttg2760
aggcctccaacggtaaaacatacagattacagagatacgctgtaaccaatactcaaccaa2820
gacctgagtcttatagagaagattacataacagccagtaacagatcctactactcaccat2880
ctggtatcatctaccctgaatcccaccaactaaacgaatctgaaaaggctgttctgtata2940
gccatttagcatcaggggcagaatctggatgggactatacctcaagatggctctctacac3000
cttctgatgccgtgagagataactactttccattgagatcactaaatacaaacaatatag3060
ttccagtagaccttaactcaattttgtacgcaaacgaagtggccatcgctgagttcctta3120
acagaactggaaacagtacaggtgcatcagattggatggatctagcaaagcaaagatcag3180
aggctatgtacgcccttatgtggaatgaaactctctggtcatactttgattacaacatga3240
catctaagactcaaaacagattcataccagtcgatgaagatgcagtttcaattgagacaa3300
ataacgctccagcaggacaacaagtctttttccatgtcgcacaatactaccctttctgga3360
ctggcgcagccccaagatccttgaaaaataatccattggctgttttgagagcatacgagc3420
gtatcgatgcttacttggacatcaagcgtggagcaattccagcgacaaacttaaagactg3480
gtcagcaatgggacgagccaaacgtctggcctccattaatgcacattctgatggaagggc3540
tgaccagagtaccagcgacatttggggaagatgatgtcgcatggacagaaatacaagact3600
tagctctaagacttggtcagagatacttagattctactttttgcacttggtacgctactg3660
gtggttcaacatctgaaacaccacagctacagggattgaacgctgaagataagggtatca3720
tgttcgaaaagtacggtgacaattctacaaacgttgctggttccggcggggagtacgaag3780
tggttgagggtttcggttggacaaatggtgttttgatgtgggtcgcagatactttcaata3840
acaagctcactagacctgattgcggtaacattaccgctgcaaatgttcactccgacggtt3900
ctcaagccagaaagagaggtgagatgtggtcagctttagaaatgcatccttacgatgcag3960
cttggactaaagagttcggtgcgagaaaggttagaagagacaaggccgaagcgagagcac4020
ttggaaatgttatgggcggcgtctgattaattaatttaccagcttactatccttcttgaa4080
aatatgcactctatatcttttagttcttaattgcaacacatagatttgctgtataacgaa4140
ttttatgctatttttttaatttggagttcggtgatgaaagtgtcacagcgaatttcctca4200
catgtagggaccgaattgtttacaagttctctgtaccaccatggagacatcaaagattga4260
aaatctatggaaagatatggacggtagcaacaagaatatagcacgagccgcggagttcat4320
ttcgttacttttgatatcgctcacaactattgcgaagcgcttcagtgaaaaaatcataag4380
gaaaagttgtaaatattattggtagtattcgtttggtaaagtagagggggtaatttttcc4440
cctttattttgttcatacattcttaaattgctttgcctctccttttggaaagctatactt4500
cggagcactgttgagcgaaggctcaggccggcagcacgcagcacgctgtatttacgtatt4560
taattttatatatttgtgcatacactactagggaagacttgaaaaaaacctaggaaatga4620
aaaaacgacacaggaagtcccgtatttactattttttccttccttttgatggggcagggc4680
ggaaatagaggataggataagcctactgcttagctgtttccgtctctacttcggtagttg4740
tctcaattgtcgtttcagtattacctttagagccgctagacgatggttgagctatttgtt4800
gagggaaaactaagttcatgtaacacacgcataacccgattaaactcatgaatagcttga4860
ttgcaggaggctggtccattggagatggtgccttattttccttataggcaacgatgatgt4920
cttcgtcggtgttcaggtagtagtgtacactctgaatcagggagaaccaggcaatgaact4980
tgttcctcaagaaaatagcggccataggcatggattggttaaccacaccagatatgcttg5040
gtgtggcagaatatagtccttttggtggcgcaattttcttgtacctgtggtagaaaggga5100
gcggttgaactgttagtatatattggcaatatcagcaaatttgaaagaaaattgtcggtg5160
aaaaacatacgaaacacaaaggtcgggccttgcaacgttattcaaagtcattgtttagtt5220
gaggaggtagcagcgg5236
<210>106
<211>6386
<212>dna
<213>人工序列(artificialsequence)
<220>
<223>由多种生物体酿酒酵母和光滑假丝酵母共同组成的序列:
<400>106
ggccgctccatggagggcacagttaagccgctaaaggcattatccgccaagtacaatttt60
ttactcttcgaagacagaaaatttgctgacattggtaatacagtcaaattgcagtactct120
gcgggtgtatacagaatagcagaatgggcagacattacgaatgcacacggtgtggtgggc180
ccaggtattgttagcggtttgaagcaggcggcggaagaagtaacaaaggaacctagaggc240
cttttgatgttagcagaattgtcatgcaagggctccctagctactggagaatatactaag300
ggtactgttgacattgcgaagagcgacaaagattttgttatcggctttattgctcaaaga360
gacatgggtggaagagatgaaggttacgattggttgattatgacacccggtgtgggttta420
gatgacaagggagacgcattgggtcaacagtatagaaccgtggatgatgtggtctctaca480
ggatctgacattattattgttggaagaggactatttgcaaagggaagggatgctaaggta540
gagggtgaacgttacagaaaagcaggctgggaagcatatttgagaagatgcggccagcaa600
aactaaaaaactgtattataagtaaatgcatgtatactaaactcacaaattagagcttca660
atttaattatatcagttattacccgggaatctcggtcgtaatgatttttataatgacgaa720
aaaaaaaaaattggaaagaaaaagcttcatggcctttataaaaaggaaccatccaatacc780
tcgccagaaccaagtaacagtattttacggggcacaaatcaagaacaataagacaggact840
gtaaagatggacgcattgaactccaaagaacaacaagagttccaaaaagtagtggaacaa900
aagcaaatgaaggatttcatgcgtttgataacttcgtataatgtatgctatacgaagtta960
tctcgagtaacacttacagagtcctatcaggaaagaataaaagccgatcaagcttcattc1020
tcaggtaacaactggagcactggcgagctttgctctactatataagataaaatatgcact1080
aaaagtttgcatttctttacataactaaaactaagacattatgcatagcttacctgatca1140
aaaagtatgtaaacttgttaacatcttcacatgtgattcatctggtcgtactttcttgcg1200
gtgcagtgtaatatttctacccacgtgactataattgagcttgaaaactgtggcgttttt1260
ccaccgatgggtccacgccagatattaaccgaagccaaaataccgatgaaatttctgaga1320
tagctcttgtaaacgacgtcaaatcttcatatgcaaggagatcttgatttctttttggta1380
gtcatctgtcgtcttgaggcgtataagaaggaggttatatctgtcctttctacaaagtat1440
tttcgagaatcttgcctctgccccttttttctttttttaaaaggtttaaaaaacataact1500
gtcttcaatatatccagtatttacgacaatatacaaacataatctctagaatgaaattca1560
tctctactttccttacttttattttagctgccgtatctgtaaccgcctctaccaactctt1620
acttctcttcctccttagcttctgcttcaccattattcaacaaagagggcagagtggtta1680
gaccaatgcgtgaaagtaacttaggcctacacgcagatcctcaaactagaaaatccagta1740
agacactttacgatttgttgtcagactttgacaatgcattttacgatgatgaaaacatga1800
tcttaggatccctagccttcggtgagaatacctactccagacagccatacgtcgcaaacg1860
ggtacataggtagccgtataccaaacatcggtttcggatacgcacttgatacacttaact1920
tgtacgctgatgcaccaggcgcacttaacaacggttggcctttgagaaacagaagattcg1980
ctggaagtttcgtttctgatttctactcactgcaagccaagttgaacagtaccaattttc2040
ctgagttagatgaaaagggttatacaactgtcatttcatcaatcccagaatggacagatt2100
tgcaattcactgtagaccttaacggcactaactggttcaatccacaatcagttctaatcg2160
atgatgttattaattacaatcagaacttgagcatgaaggatggaattgttagcacaaaca2220
tggattggttgaacggaatgataaacattaagtctgaagtctgggcacacagaaagatcc2280
attccttgggtatcacacgtctggaaatctcattgaaccttgatgctttacctgacgagt2340
tcactgaactaccagttacaatttacgatatcatagatttcaacacttctcatagaacaa2400
cactatatgagaaaggtcaagatgaagataacaaagcgatttacatgattgtaaacccag2460
aaaacgtaccatactcaaacgcagttgtgtactctacttgtacaatcaagggcactgaaa2520
acaacttttctccttacaacttcacatcagatgatagaattgcgagaaactacatgacaa2580
acctaacagaagagaacccaaaagtcgttatttacaagtacacatcagtggtttcctcag2640
aatataataatgacgagccaaatcctaacgttaacttgaagttcgcctcaaacatcgcta2700
acaccgcaaaaggaaactataagtctttattgtccaatcacaaaagagcttggtacgatt2760
tgtacaatgatgcctttatcgaaattccatctgatagtttattggaaatgaccgcaagat2820
ctagtctcttccatttgcttgcgaacacacgtcaatacaacgtttctacaactagaggat2880
taccagtcggcgttggtggtctttcttcagactcttacggggggatggtcttttgggacg2940
cagacgtatggatggctccagctctcctacctttctttccaaacatagcaatgaacatga3000
ataactacagaaacgctactcatcaacaagccattgaaaatgctaagcaatacaattacc3060
caggcgccgtttacccttggacatctggtagatatgcgaattgtacctctaccggtcctt3120
gcatcgactacgaataccatattaatgtagatatcgctttggcaagtttctctatttaca3180
tgaacggtgccgagggtgccgacgaagattaccttagattcacaacatggccaatggtca3240
aggatgctgcagtgttcttcaaggcctacgtcaaatacaatgaaacacttggagagtacg3300
aaacttataatttaactgacccagatgaatttgctaatcatgtgaataatggagctttca3360
caaatgcaggaatcaaaactttgttgaaatgggcaacagacattggtactcaccttgggg3420
aggaagttgatcctaagtggatggaaattgctgataacatacatattcctagaagtgact3480
ctaacattactttggagtactctggcatgaatagctccgttgagattaagcaggctgacg3540
ttacattgatggtataccctttaggttacatcaacgatgaatctatactaaacaacgcaa3600
tcaaggatttgtattactactctgaacgtcagtctgcctctgggccagctatgacttacc3660
cagtgttcgtcgcagctgccgcttccctcttgaaccacggttcatcatcacagagttacc3720
tttacaaatctgttcttccatatctgagatcaccatttgctcaatttagcgaacaatcag3780
atgacaatttcttaactaacggtttgacacaaccagcattcccatttttgaccgctaatg3840
gtggtttcttgcaatctatattatttggattaaccggattgagatacagttacgaagtta3900
ctcctagaaccaaaaagatttcaagactactcaagtttgatccagtcaagttgccactgc3960
tacctggcggaattgcaattagaaatttcaaatacatgggacaagtactggatatcataa4020
tagacgataacaatggtacaatagctcataagggtggggataagccaattagaatcaagg4080
ttccaaatagagatattctccacgacagaaatatcacttccgcactatactcaaagagag4140
atgacgatttatcagcgacagatgactactacggtacatacttcacactatacccaaatg4200
aggaactggtgattcctctttacgatactaagttaaacatcgacggtaacatagccgaat4260
ctaaacaaataactaatttgactgccggagttccaggtgacgttggtttctccgcattag4320
atgggaacaattacacacactggcagccattcgataaatcagataatgctaagttgctaa4380
ttgacttaggttttaacagcactcatgtcattaaaaagggtattattttgtggggtcaaa4440
gaccagcgaaaaacatttctttgtcagttttgccacattcagagagaattgagcaactat4500
ttgcgaacatcacagatcttttagaaacatcatctataactaagggcggctctttgttaa4560
accagatgttaggccaaacacaatcaaacgtgaccgcagaaattgatgatgatatcctcg4620
ctttactgaactggaaaggtgacgatctggaccaactcatcccttatttgcctgatatgc4680
atctcctacaagagaaattcattcctattttgaaggattaccctattaagccaaatcaga4740
gatactataaggaaatcattgacgatgacataatcaagttattaccatcaaacacaactg4800
aatttacaatcgattacaattctatccctggcggggaaaagcgtgcaagatacgttgttc4860
tgacagttcacggcacctatgatgacgatgatgatcttaaaggtgcaacaatcaaggaaa4920
ttgttctgcaggaatgattaattaatttaccagcttactatccttcttgaaaatatgcac4980
tctatatcttttagttcttaattgcaacacatagatttgctgtataacgaattttatgct5040
atttttttaatttggagttcggtgatgaaagtgtcacagcgaatttcctcacatgtaggg5100
accgaattgtttacaagttctctgtaccaccatggagacatcaaagattgaaaatctatg5160
gaaagatatggacggtagcaacaagaatatagcacgagccgcggagttcatttcgttact5220
tttgatatcgctcacaactattgcgaagcgcttcagtgaaaaaatcataaggaaaagttg5280
taaatattattggtagtattcgtttggtaaagtagagggggtaatttttcccctttattt5340
tgttcatacattcttaaattgctttgcctctccttttggaaagctatacttcggagcact5400
gttgagcgaaggctcaggccggcagcacgcagcacgctgtatttacgtatttaattttat5460
atatttgtgcatacactactagggaagacttgaaaaaaacctaggaaatgaaaaaacgac5520
acaggaagtcccgtatttactattttttccttccttttgatggggcagggcggaaataga5580
ggataggataagcctactgcttagctgtttccgtctctacttcggtagttgtctcaattg5640
tcgtttcagtattacctttagagccgctagacgatggttgagctatttgttgagggaaaa5700
ctaagttcatgtaacacacgcataacccgattaaactcatgaatagcttgattgcaggag5760
gctggtccattggagatggtgccttattttccttataggcaacgatgatgtcttcgtcgg5820
tgttcaggtagtagtgtacactctgaatcagggagaaccaggcaatgaacttgttcctca5880
agaaaatagcggccataggcatggattggttaaccacaccagatatgcttggtgtggcag5940
aatatagtccttttggtggcgcaattttcttgtacctgtggtagaaagggagcggttgaa6000
ctgttagtatatattggcaatatcagcaaatttgaaagaaaattgtcggtgaaaaacata6060
cgaaacacaaaggtcgggccttgcaacgttattcaaagtcattgtttagttgaggaggta6120
gcagcggagtatatgtattccttttttttgcctatggatgttgtaccatgcccattctgc6180
tcaagcttttgttaaaattatttttcagtattttttcttccatgttgcgcgttacgagaa6240
cagaagcgacagataaccgcaatcatacaactagcgctactgcggggtgtaaaaagcaca6300
agaactaagccaagatcacaacagttatcgataaaatagcagtgtttgcatggccattga6360
gaaggacaacattggcgtgcggcatg6386
<210>107
<211>6535
<212>dna
<213>人工序列(artificialsequence)
<220>
<223>由多种生物体酿酒酵母、光滑假丝酵母和构巢曲霉共同组成的序列:
<400>107
ggccgcgaaggtgctattgttggtattagaggtggtgttattggtgtcggtactgacatt60
ggtggttccattagagtcccagctgctttcaactttttatacggtttgagaccatctcac120
ggtagattgccatatgctaaaatggctaactctatggaaggtcaagaaaccgttcactcc180
gtcgttggtcctatcactcactccgtcgaagacttgagattgttcaccaaatctgtcttg240
ggtcaagaaccttggaagtacgactctaaggtcatcccaatgccatggagacaatctgaa300
tctgacatcattgcctctaagattaagaatggtggtttgaacattggttattacaatttc360
gacggtaacgtcttgccacacccaccaattttacgtggtgtcgaaactaccgttgccgct420
ttggccaaggctggtcacaccgttactccatggactccatacaagcatgatttcggtcat480
gacttgatttcccacatctatgctgctgatggttctgccgacgtcatgagagacatttct540
gcctctggtgagccagccatccctaacattaaggacttgttgaacccaaatattaaggct600
gttaacatgaacgaattgtgggacactcatttacaaaagtggaactatcaaatggaatac660
ttggaaaagtggcgtgaagctgaagaaaaagctggtaaggaattggacgctattatcgct720
ccaattactcctaccgccgctgtcagacacgatcaattcagatactacggttacgcctcc780
gttattaacttattggatttcacctctgttgtcgtcccagtcactttcgctgataagaat840
attgataagaagaacgaatcttttaaagctgtttccgaattggatgctttggttcaagaa900
gaatacgacccagaggcttatcacggtgctcctgttgctgttcaagttattggtagaaga960
ttgtccgaagagagaactttggctatcgccgaagaagtcggtaaattgttgggtaacgtc1020
gtcactccataagcgaatttcttatgatttatgatttttattattaaataagttataaaa1080
aaaataagtgtatacaaattttaaagtgactcttaggttttaaaacgaaaattcttattc1140
ttgagtaactctttcctgtaggtcaggttgctttctcaggtatagcatgaggtcgctctt1200
attgaccacacctctaccggcatgccgagcaaatgcctgcaaatcgctccccatttcacc1260
caattgtagatatgctaactccagcaatgagttgatgaatctcggtgtgtattttatgtc1320
ctcagaggacaacacataacttcgtataatgtatgctatacgaagttatctcgagtaaca1380
cttacagagtcctatcaggaaagaataaaagccgatcaagcttcattctcaggtaacaac1440
tggagcactggcgagctttgctctactatataagataaaatatgcactaaaagtttgcat1500
ttctttacataactaaaactaagacattatgcatagcttacctgatcaaaaagtatgtaa1560
acttgttaacatcttcacatgtgattcatctggtcgtactttcttgcggtgcagtgtaat1620
atttctacccacgtgactataattgagcttgaaaactgtggcgtttttccaccgatgggt1680
ccacgccagatattaaccgaagccaaaataccgatgaaatttctgagatagctcttgtaa1740
acgacgtcaaatcttcatatgcaaggagatcttgatttctttttggtagtcatctgtcgt1800
cttgaggcgtataagaaggaggttatatctgtcctttctacaaagtattttcgagaatct1860
tgcctctgccccttttttctttttttaaaaggtttaaaaaacataactgtcttcaatata1920
tccagtatttacgacaatatacaaacataatctctagaatgaaattcatctctactttcc1980
ttacttttattttagctgccgtatctgtaaccgcctctaccaactcttacttctcttcct2040
ccttagcttctgcttcaccattattcaacaaagagggcagagtggttagaccaatgcgtg2100
aaagtaacttaggcctacacgcagatcctcaaactagaaaatccagtaagacactttacg2160
atttgttgtcagactttgacaatgcattttacgatgatgaaaacatgatcttaggatccc2220
tagccttcggtgagaatacctactccagacagccatacgtcgcaaacgggtacataggta2280
gccgtataccaaacatcggtttcggatacgcacttgatacacttaacttgtacgctgatg2340
caccaggcgcacttaacaacggttggcctttgagaaacagaagattcgctggaagtttcg2400
tttctgatttctactcactgcaagccaagttgaacagtaccaattttcctgagttagatg2460
aaaagggttatacaactgtcatttcatcaatcccagaatggacagatttgcaattcactg2520
tagaccttaacggcactaactggttcaatccacaatcagttctaatcgatgatgttatta2580
attacaatcagaacttgagcatgaaggatggaattgttagcacaaacatggattggttga2640
acggaatgataaacattaagtctgaagtctgggcacacagaaagatccattccttgggta2700
tcacacgtctggaaatctcattgaaccttgatgctttacctgacgagttcactgaactac2760
cagttacaatttacgatatcatagatttcaacacttctcatagaacaacactatatgaga2820
aaggtcaagatgaagataacaaagcgatttacatgattgtaaacccagaaaacgtaccat2880
actcaaacgcagttgtgtactctacttgtacaatcaagggcactgaaaacaacttttctc2940
cttacaacttcacatcagatgatagaattgcgagaaactacatgacaaacctaacagaag3000
agaacccaaaagtcgttatttacaagtacacatcagtggtttcctcagaatataataatg3060
acgagccaaatcctaacgttaacttgaagttcgcctcaaacatcgctaacaccgcaaaag3120
gaaactataagtctttattgtccaatcacaaaagagcttggtacgatttgtacaatgatg3180
cctttatcgaaattccatctgatagtttattggaaatgaccgcaagatctagtctcttcc3240
atttgcttgcgaacacacgtcaatacaacgtttctacaactagaggattaccagtcggcg3300
ttggtggtctttcttcagactcttacggggggatggtcttttgggacgcagacgtatgga3360
tggctccagctctcctacctttctttccaaacatagcaatgaacatgaataactacagaa3420
acgctactcatcaacaagccattgaaaatgctaagcaatacaattacccaggcgccgttt3480
acccttggacatctggtagatatgcgaattgtacctctaccggtccttgcatcgactacg3540
aataccatattaatgtagatatcgctttggcaagtttctctatttacatgaacggtgccg3600
agggtgccgacgaagattaccttagattcacaacatggccaatggtcaaggatgctgcag3660
tgttcttcaaggcctacgtcaaatacaatgaaacacttggagagtacgaaacttataatt3720
taactgacccagatgaatttgctaatcatgtgaataatggagctttcacaaatgcaggaa3780
tcaaaactttgttgaaatgggcaacagacattggtactcaccttggggaggaagttgatc3840
ctaagtggatggaaattgctgataacatacatattcctagaagtgactctaacattactt3900
tggagtactctggcatgaatagctccgttgagattaagcaggctgacgttacattgatgg3960
tataccctttaggttacatcaacgatgaatctatactaaacaacgcaatcaaggatttgt4020
attactactctgaacgtcagtctgcctctgggccagctatgacttacccagtgttcgtcg4080
cagctgccgcttccctcttgaaccacggttcatcatcacagagttacctttacaaatctg4140
ttcttccatatctgagatcaccatttgctcaatttagcgaacaatcagatgacaatttct4200
taactaacggtttgacacaaccagcattcccatttttgaccgctaatggtggtttcttgc4260
aatctatattatttggattaaccggattgagatacagttacgaagttactcctagaacca4320
aaaagatttcaagactactcaagtttgatccagtcaagttgccactgctacctggcggaa4380
ttgcaattagaaatttcaaatacatgggacaagtactggatatcataatagacgataaca4440
atggtacaatagctcataagggtggggataagccaattagaatcaaggttccaaatagag4500
atattctccacgacagaaatatcacttccgcactatactcaaagagagatgacgatttat4560
cagcgacagatgactactacggtacatacttcacactatacccaaatgaggaactggtga4620
ttcctctttacgatactaagttaaacatcgacggtaacatagccgaatctaaacaaataa4680
ctaatttgactgccggagttccaggtgacgttggtttctccgcattagatgggaacaatt4740
acacacactggcagccattcgataaatcagataatgctaagttgctaattgacttaggtt4800
ttaacagcactcatgtcattaaaaagggtattattttgtggggtcaaagaccagcgaaaa4860
acatttctttgtcagttttgccacattcagagagaattgagcaactatttgcgaacatca4920
cagatcttttagaaacatcatctataactaagggcggctctttgttaaaccagatgttag4980
gccaaacacaatcaaacgtgaccgcagaaattgatgatgatatcctcgctttactgaact5040
ggaaaggtgacgatctggaccaactcatcccttatttgcctgatatgcatctcctacaag5100
agaaattcattcctattttgaaggattaccctattaagccaaatcagagatactataagg5160
aaatcattgacgatgacataatcaagttattaccatcaaacacaactgaatttacaatcg5220
attacaattctatccctggcggggaaaagcgtgcaagatacgttgttctgacagttcacg5280
gcacctatgatgacgatgatgatcttaaaggtgcaacaatcaaggaaattgttctgcagg5340
aatgattaattaatttaccagcttactatccttcttgaaaatatgcactctatatctttt5400
agttcttaattgcaacacatagatttgctgtataacgaattttatgctatttttttaatt5460
tggagttcggtgatgaaagtgtcacagcgaatttcctcacatgtagggaccgaattgttt5520
acaagttctctgtaccaccatggagacatcaaagattgaaaatctatggaaagatatgga5580
cggtagcaacaagaatatagcacgagccgcggagttcatttcgttacttttgatatcgct5640
cacaactattgcgaagcgcttcagtgaaaaaatcataaggaaaagttgtaaatattattg5700
gtagtattcgtttggtaaagtagagggggtaatttttcccctttattttgttcatacatt5760
cttaaattgctttgcctctccttttggaaagctatacttcggagcactgttgagcgaagg5820
ctcaggccggcagcacgcagcacgctgtatttacgtatttaattttatatatttgtgcat5880
acactactagggaagacttgaaaaaaacctaggaaatgaaaaaacgacacaggaagtccc5940
gtatttactattttttccttccttttgatggggcagggcggaaatagaggataggataag6000
cctactgcttagctgtttccgtctctacttcggtagttgtctcaattgtcgtttcagtat6060
tacctttagagccgctagacgatggttgagctatttgttgagggaaaactaagttcatgt6120
aacacacgcataacccgattaaactcatgaatagcttgattgcaggaggctggtccattg6180
gagatggtgccttattttccttataggcaacgatgatgtcttcgtcggtgttcaggtagt6240
agtgtacactctgaatcagggagaaccaggcaatgaacttgttcctcaagaaaatagcgg6300
ccataggcatggattggttaaccacaccagatatgcttggtgtggcagaatatagtcctt6360
ttggtggcgcaattttcttgtacctgtggtagaaagggagcggttgaactgttagtatat6420
attggcaatatcagcaaatttgaaagaaaattgtcggtgaaaaacatacgaaacacaaag6480
gtcgggccttgcaacgttattcaaagtcattgtttagttgaggaggtagcagcgg6535
<210>108
<211>605
<212>prt
<213>小孢根霉(rhizopusmicrosporus)
<400>108
metlysleumetasnprosermetlysalatyrvalphepheileleu
151015
sertyrpheserleuleuvalserseralaalavalprothrserala
202530
alavalglnvalglusertyrasntyraspglythrthrphesergly
354045
argilephevallysasnilealatyrserlysvalvalthrvalile
505560
tyrseraspglyseraspasntrpasnasnasnasnasnlysvalser
65707580
alaalatyrserglualaileserglyserasntyrglutyrtrpthr
859095
pheseralalysleuserglyilelysglnphetyrvallystyrglu
100105110
valserglyserthrtyrtyraspasnasnglythrlysasntyrgln
115120125
valglnalathrseralathrserthrthralathralathrthrthr
130135140
thralathrglythrthrthrthrserthrglyprothrserthrala
145150155160
servalserpheprothrglyasnserthrilesersertrpilelys
165170175
asnglnglugluileserargphealametleuargasnileasnpro
180185190
proglyseralathrglypheilealaalaserleuserthralagly
195200205
proasptyrtyrtyrsertrpthrargaspseralaleuthralaasn
210215220
valilealatyrglutyrasnthrthrphethrglyasnthrthrleu
225230235240
leulystyrleulysasptyrvalthrpheservallysserglnser
245250255
valserthrvalcysasncysleuglygluprolyspheasnalaasp
260265270
glyserserphethrglyprotrpglyargproglnasnaspglypro
275280285
alagluargalavalthrphemetleuilealaaspsertyrleuthr
290295300
glnthrlysaspalasertyrvalthrglythrleulysproalaile
305310315320
phelysaspleuasptyrvalvalservaltrpserasnglycystyr
325330335
aspleutrpglugluvalasnglyvalhisphetyrthrleumetval
340345350
metarglysglyleuileleuglyalaaspphealaalaargasngly
355360365
aspserserargalaserthrtyrlysglnthralaserthrmetglu
370375380
serlysileserserphetrpseraspserasnasntyrvalglnval
385390395400
serglnservalthralaglyvalserlyslysglyleuaspvalser
405410415
thrleuleualaalaasnileglyserleuproaspglyphephethr
420425430
proglyserglulysileleualathralavalalaleugluasnala
435440445
phealaserleutyrproileasnserasnleuprosertyrleugly
450455460
asnserileglyargtyrprogluaspthrtyrasnglyasnglyasn
465470475480
serglnglyasnprotrppheleualavalasnalatyralagluleu
485490495
tyrtyrargalailelysglutrpileserasnglylysvalthrval
500505510
serasnileserleuprophephelyslyspheaspserseralathr
515520525
serglylysthrtyrthralaglythrserasppheasnasnleuala
530535540
glnasnilealaleuglyalaaspargpheleuserthrvallysphe
545550555560
hisalatyrthrasnglyserleusergluglutyraspargserthr
565570575
glymetserthrglyalaargaspleuthrtrpserhisalaserleu
580585590
ilethrvalalatyralalysalaglyserproalaala
595600605
<210>109
<211>604
<212>prt
<213>戴尔根霉(rhizopusdelemar)
<400>109
metglnleupheasnleuproleulysvalserphepheleuvalleu
151015
sertyrpheserleuleuvalseralaalaserileproserserala
202530
servalglnleuaspsertyrasntyraspglyserthrphesergly
354045
lysiletyrvallysasnilealatyrserlyslysvalthrvalile
505560
tyralaaspglyseraspasntrpasnasnasnglyasnthrileala
65707580
alasertyrseralaproileserglyserasntyrglutyrtrpthr
859095
pheseralaserileasnglyilelysgluphetyrilelystyrglu
100105110
valserglylysthrtyrtyraspasnasnasnseralaasntyrgln
115120125
valserthrserlysprothrthrthrthralathralathrthrthr
130135140
thralaproserthrserthrthrthrproproserserserglupro
145150155160
alathrpheprothrglyasnserthrilesersertrpilelyslys
165170175
glngluglyileserargphealametleuargasnileasnpropro
180185190
glyseralathrglypheilealaalaserleuserthralaglypro
195200205
asptyrtyrtyralatrpthrargaspalaalaleuthrserasnval
210215220
ilevaltyrglutyrasnthrthrleuserglyasnlysthrileleu
225230235240
asnvalleulysasptyrvalthrpheservallysthrglnserthr
245250255
serthrvalcysasncysleuglygluprolyspheasnproaspgly
260265270
serglytyrthrglyalatrpglyargproglnasnaspglyproala
275280285
gluargalathrthrpheileleuphealaaspsertyrleuthrgln
290295300
thrlysaspalasertyrvalthrglythrleulysproalailephe
305310315320
lysaspleuasptyrvalvalasnvaltrpserasnglycyspheasp
325330335
leutrpglugluvalasnglyvalhisphetyrthrleumetvalmet
340345350
arglysglyleuleuleuglyalaaspphealalysargasnglyasp
355360365
serthrargalaserthrtyrserserthralaserthrilealaasn
370375380
lysileserserphetrpvalserserasnasntrpileglnvalser
385390395400
glnservalthrglyglyvalserlyslysglyleuaspvalserthr
405410415
leuleualaalaasnleuglyservalaspaspglyphephethrpro
420425430
glyserglulysileleualathralavalalavalgluaspserphe
435440445
alaserleutyrproileasnlysasnleuprosertyrleuglyasn
450455460
serileglyargtyrprogluaspthrtyrasnglyasnglyasnser
465470475480
glnglyasnprotrppheleualavalthrglytyralagluleutyr
485490495
tyrargalailelysglutrpileglyasnglyglyvalthrvalser
500505510
serileserleuprophephelyslyspheaspserseralathrser
515520525
glylyslystyrthrvalglythrserasppheasnasnleualagln
530535540
asnilealaleualaalaaspargpheleuserthrvalglnleuhis
545550555560
alahisasnasnglyserleualagluglupheaspargthrthrgly
565570575
leuserthrglyalaargaspleuthrtrpserhisalaserleuile
580585590
thralasertyralalysalaglyalaproalaala
595600
<210>110
<211>1440
<212>dna
<213>蜡样芽孢杆菌(bacilluscereus)
<400>110
atgacaacatcaaatacctacaaattctatctaaacggtgaatggagagaatcttcctct60
ggagaaactattgagataccatcaccatacttacatgaagtgatcggacaggttcaagca120
atcactagaggagaggttgacgaagcgattgctagcgctaaggaagcacagaaatcttgg180
gctgaggcatctctacaagatagagctaagtacttgtacaaatgggcagatgaattggta240
aacatgcaagacgaaatcgccgatatcatcatgaaggaagtgggcaagggttacaaagac300
gctaaaaaggaggttgttagaaccgccgatttcatcagatacaccattgaagaggcactc360
catatgcacggtgaatccatgatgggcgattcatttcctggtggaacaaaatctaagcta420
gcaataatccaaagagcgcctctgggtgtagtcttagccatcgctccattcaattaccct480
gtaaacctttctgctgcaaaattggcaccagccttaattatgggtaacgctgtgatattc540
aagccagcaactcagggtgctatttccggcatcaaaatggttgaagctttgcataaggct600
ggtttgccaaagggtttggttaacgttgccacaggtagaggtagcgtcataggcgattat660
ttggtcgaacacgaagggataaacatggtttccttcaccggtggcactaacactggtaag720
catttagcaaaaaaggcctcaatgattccattagtcttggaacttggtggcaaagatcca780
ggcatcgttcgtgaagatgcagacctacaagatgctgcgaatcatatcgtatctggtgcg840
ttcagttactcagggcagagatgtacagccattaagagagtccttgttcatgaaaatgtt900
gctgatgaactggtatcattggttaaggaacaagtggcaaagctttctgtgggatcacca960
gagcaagattcaacaattgttcctctgattgacgataagtccgctgattttgttcagggt1020
ttagtggacgatgcagtcgaaaagggcgctacaattgtcattgggaacaagagagaacgt1080
aacctaatctacccaacattgattgatcacgtcacagaggaaatgaaagttgcctgggag1140
gaaccattcggtcctattcttccaattattagagttagtagcgacgagcaagctattgaa1200
attgcaaataagagtgagttcggattacaagcttctgtgtttaccaaagacataaacaag1260
gcattcgcaatcgcaaataagattgagactggttcagtgcaaatcaacggtagaacagag1320
agaggaccagatcactttccttttatcggggttaagggatctgggatgggtgcccaaggc1380
atcagaaagtctttggaatctatgactagagaaaaagttactgtcttaaatctcgtatga1440
<210>111
<211>479
<212>prt
<213>蜡样芽孢杆菌(bacilluscereus)
<400>111
metthrthrserasnthrtyrlysphetyrleuasnglyglutrparg
151015
gluserserserglygluthrilegluileproserprotyrleuhis
202530
gluvalileglyglnvalglnalailethrargglygluvalaspglu
354045
alailealaseralalysglualaglnlyssertrpalaglualaser
505560
leuglnaspargalalystyrleutyrlystrpalaaspgluleuval
65707580
asnmetglnaspgluilealaaspileilemetlysgluvalglylys
859095
glytyrlysaspalalyslysgluvalvalargthralaasppheile
100105110
argtyrthrilegluglualaleuhismethisglyglusermetmet
115120125
glyaspserpheproglyglythrlysserlysleualaileilegln
130135140
argalaproleuglyvalvalleualailealapropheasntyrpro
145150155160
valasnleuseralaalalysleualaproalaleuilemetglyasn
165170175
alavalilephelysproalathrglnglyalaileserglyilelys
180185190
metvalglualaleuhislysalaglyleuprolysglyleuvalasn
195200205
valalathrglyargglyservalileglyasptyrleuvalgluhis
210215220
gluglyileasnmetvalserphethrglyglythrasnthrglylys
225230235240
hisleualalyslysalasermetileproleuvalleugluleugly
245250255
glylysaspproglyilevalarggluaspalaaspleuglnaspala
260265270
alaasnhisilevalserglyalaphesertyrserglyglnargcys
275280285
thralailelysargvalleuvalhisgluasnvalalaaspgluleu
290295300
valserleuvallysgluglnvalalalysleuservalglyserpro
305310315320
gluglnaspserthrilevalproleuileaspasplysseralaasp
325330335
phevalglnglyleuvalaspaspalavalglulysglyalathrile
340345350
valileglyasnlysarggluargasnleuiletyrprothrleuile
355360365
asphisvalthrgluglumetlysvalalatrpglugluprophegly
370375380
proileleuproileileargvalserseraspgluglnalaileglu
385390395400
ilealaasnlysserglupheglyleuglnalaservalphethrlys
405410415
aspileasnlysalaphealailealaasnlysilegluthrglyser
420425430
valglnileasnglyargthrgluargglyproasphispheprophe
435440445
ileglyvallysglyserglymetglyalaglnglyilearglysser
450455460
leuglusermetthrargglulysvalthrvalleuasnleuval
465470475
<210>112
<211>577
<212>dna
<213>酿酒酵母(saccharomycescerevisiae)
<400>112
taacacttacagagtcctatcaggaaagaataaaagccgatcaagcttcattctcaggta60
acaactggagcactggcgagctttgctctactatataagataaaatatgcactaaaagtt120
tgcatttctttacataactaaaactaagacattatgcatagcttacctgatcaaaaagta180
tgtaaacttgttaacatcttcacatgtgattcatctggtcgtactttcttgcggtgcagt240
gtaatatttctacccacgtgactataattgagcttgaaaactgtggcgtttttccaccga300
tgggtccacgccagatattaaccgaagccaaaataccgatgaaatttctgagatagctct360
tgtaaacgacgtcaaatcttcatatgcaaggagatcttgatttctttttggtagtcatct420
gtcgtcttgaggcgtataagaaggaggttatatctgtcctttctacaaagtattttcgag480
aatcttgcctctgccccttttttctttttttaaaaggtttaaaaaacataactgtcttca540
atatatccagtatttacgacaatatacaaacataatc577
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