一种可用于昆虫基因编辑的CRISPR/Cas9载体的制作方法

本发明属于生物
技术领域：
，具体涉及一种可用于昆虫基因编辑的crispr/cas9载体。
背景技术：
：crispr(clusteredregularlyinterspacedshortpalindromicrepeats)技术是近年发展起来的新型高效基因编辑技术，来源于对细菌免疫机制的研究。在噬菌体感染细菌的过程中，细菌把噬菌体核酸的部分片段整合到自身基因组中，并进化出cas(crispr-associated)核酸酶。当噬菌体再次感染时，cas核酸酶在这些片段(称为grna)的引导下结合到噬菌体核酸的相应部位并切割核酸，从而阻止噬菌体的侵染。研究人员利用此原理，发展了一种基因编辑技术，即通过grna引导cas9核酸酶到目的基因的把序列上，cas9切割双链dna，通过非同源末端连接机制引入缺失插入等突变，从而破坏基因功能。crispr/cas9基因编辑技术需要相应的载体来实现。基本过程是从化脓链球菌(streptococcuspyogenes)中得到cas9基因序列并经过特定表达宿主密码子优化后克隆到相应的表达载体，以及克隆小rna启动子驱动的grna骨架序列到同一表达载体。crispr/cas9基因编辑技术对靶基因编辑位点的选择是由grna确定的，而grna的设计需要遵循一定的规则。第一，转录起始碱基为鸟嘌呤g；第二，中间序列长度为20个碱基；第三，靶基因中与上述20个碱基区域下游三碱基必须为ngg，称为pam序列，其中n代表任一碱基。农业害虫控制是农业生产中一个非常重要的环节。对种植业来说，我国目前普遍使用化学农药，保证了粮食及果蔬的供给，但在其生产与使用过程中同样对人体健康、生态环境及物种多样性造成了深刻的影响，因此本领域迫切需要开发新的农业害虫控制手段。技术实现要素：本发明的目的就是提供一种可用于昆虫基因编辑的crispr/cas9载体。在本发明的第一方面，提供了一种用于编辑昆虫细胞基因的试剂，所述试剂包括：(i)靶向abc转运蛋白基因的sgrna或用于表达所述sgrna的表达载体；和任选的(ii)表达cas9蛋白或表达cas9蛋白的表达盒。在另一优选例中，所述昆虫为小菜蛾、二化螟。在另一优选例中，所述abc转运蛋白基因为abch1。在另一优选例中，所述sgrna包括含有其对应识别区序列的核苷酸。在另一优选例中，所述sgrna的序列为sgrna识别区序列或相应的全长sgrna序列。在另一优选例中，所述sgrna的序列包括选自下组的序列：(1)sgrna识别区序列；或(2)相应的全长sgrna序列。在另一优选例中，所述的全长sgrna序列从5’端至3’端依次含有sgrna识别区序列和sgrna框架序列。在另一优选例中，所述sgrna识别区序列具有seqidno.:1(gtcgccgccgggcgacgaga)所示的核苷酸序列。在另一优选例中，所述sgrna框架序列具有seqidno.:2(gttttagagctagaaatagcaagttaaaataaggctagtccgttatcaacttgaaaaagtggcaccgagtcggtgc)所示的核苷酸序列。在另一优选例中，所述sgrna具有seqidno.:3(gtcgccgccgggcgacgagagttttagagctagaaatagcaagttaaaataaggctagtccgttatcaacttgaaaaagtggcaccgagtcggtgc)所示的核苷酸序列。在另一优选例中，所述靶向abc转运蛋白基因的sgrna为经过或未经过化学基团修饰的。在另一优选例中，所述abc转运蛋白基因来源于昆虫，较佳地，来源于小菜蛾。在本发明的第二方面，提供了一种试剂盒，所述试剂盒含有：(1)第一容器以及位于所述第一容器内的第一表达载体，所述第一表达载体含有第一表达盒，所述第一表达盒用于表达所述靶向abc转运蛋白基因的sgrna；和任选的(2)第二容器以及位于所述第二容器内的第二表达载体，所述第二表达载体含有第二表达盒，所述第二表达盒用于表达cas9蛋白的表达盒。在另一优选例中，所述靶向abc转运蛋白基因的sgrna的识别区序列具有seqidno.:1所示的核苷酸序列。在另一优选例中，所述第一和第二表达载体为独立的或相连的。在另一优选例中，所述第一和第二表达载体位于相同或不同的容器内。在另一优选例中，所述第一和第二表达盒位于相同或不同的载体上。在另一优选例中，所述的载体选自下组：dna、rna、质粒、慢病毒载体、腺病毒载体、逆转录病毒载体、转座子、其他基因转移系统、或其组合。在另一优选例中，所述表达载体包括pac-sgrna-cas9。在另一优选例中，所述表达载体的序列如seqidno.:4(ggaaaaatgatgtgacagtggaaatgagaagtaggtgcatctgcaaagatttgattcagagttgatgccattcatgattttttttagaagtatcatttgtgtttttagaatatagaattaattccttgaagacgaaagggcctcgtgatacgcctatttttataggttaatgtcatgataataatggtttcttagacgtcaggtggcacttttcggggaaatgtgcgcggaacccctatttgtttatttttctaaatacattcaaatatgtatccgctcatgagacaataaccctgataaatgcttcaataatattgaaaaaggaagagtatgagtattcaacatttccgtgtcgcccttattcccttttttgcggcattttgccttcctgtttttgctcacccagaaacgctggtgaaagtaaaagatgctgaagatcagttgggtgcacgagtgggttacatcgaactggatctcaacagcggtaagatccttgagagttttcgccccgaagaacgttttccaatgatgagcacttttaaagttctgctatgtggcgcggtattatcccgtgttgacgccgggcaagagcaactcggtcgccgcatacactattctcagaatgacttggttgagtactcaccagtcacagaaaagcatcttacggatggcatgacagtaagagaattatgcagtgctgccataaccatgagtgataacactgcggccaacttacttctgacaacgatcggaggaccgaaggagctaaccgcttttttgcacaacatgggggatcatgtaactcgccttgatcgttgggaaccggagctgaatgaagccataccaaacgacgagcgtgacaccacgatgcctgcagcaatggcaacaacgttgcgcaaactattaactggcgaactacttactctagcttcccggcaacaattaatagactggatggaggcggataaagttgcaggaccacttctgcgctcggcccttccggctggctggtttattgctgataaatctggagccggtgagcgtgggtctcgcggtatcattgcagcactggggccagatggtaagccctcccgtatcgtagttatctacacgacggggagtcaggcaactatggatgaacgaaatagacagatcgctgagataggtgcctcactgattaagcattggtaactgtcagaccaagtttactcatatatactttagattgatttaaaacttcatttttaatttaaaaggatctaggtgaagatcctttttgataatctcatgaccaaaatcccttaacgtgagttttcgttccactgagcgtcagaccccgtagaaaagatcaaaggatcttcttgagatcctttttttctgcgcgtaatctgctgcttgcaaacaaaaaaaccaccgctaccagcggtggtttgtttgccggatcaagagctaccaactctttttccgaaggtaactggcttcagcagagcgcagataccaaatactgtccttctagtgtagccgtagttaggccaccacttcaagaactctgtagcaccgcctacatacctcgctctgctaatcctgttaccagtggctgctgccagtggcgataagtcgtgtcttaccgggttggactcaagacgatagttaccggataaggcgcagcggtcgggctgaacggggggttcgtgcacacagcccagcttggagcgaacgacctacaccgaactgagatacctacagcgtgagcattgagaaagcgccacgcttcccgaagggagaaaggcggacaggtatccggtaagcggcagggtcggaacaggagagcgcacgagggagcttccagggggaaacgcctggtatctttatagtcctgtcgggtttcgccacctctgacttgagcgtcgatttttgtgatgctcgtcaggggggcggagcctatggaaaaacgccagcaacgcggcctttttacggttcctggccttttgctggctggtaagagccgcgagcgatccttgaagctgtccctgatggtcgtcatctacctgcctggacagcatggcctgcaacgcgggcatcccgatgccgccggaagcgagaagaatcataatggggaaggccatccagcctcgcgtcgcgaacgccagcaagacgtagcccagcgcgtcggccgccatgccggcgataatggcctgcttctcgccgaaacgtttggtggcgggaccagtgacgaaggcttgagcgagggcgtgcaagattccgaataccgcaagcgacaggccgatcatcgtcgcgctccagcgaaagcggtcctcgccgaaaatgacccagagcgctgccggcacctgtcctacgagttgcatgataaagaagacagtcataagtgcggcgacgatagtcatgccccgcgcccaccggaaggagctgactgggttgaaggctctcaagggcatcggtcgactgatcataatcagccataccacatttgtagaggttttacttgctttaaaaaacctcccacacctccccctgaacctgaaacataaaatgaatgcaattgttgttgttaacttgtttattgcagcttataatggttacaaataaagcaatagcatcacaaatttcacaaataaagcatttttttcactgcattctagttgtggtttgtccaaactcatcaatgtatcttatcatgtctggatccctcgagtcaggcaccgggcttgcgggtcatgcaccaggtgcgcggtccttcgggcacctcgacgtcggcggtgacggtgaagccgagccgctcgtagaaggggaggttgcggggcgcggaggtctccaggaaggcgggcaccccggcgcgctcggccgcctccactccggggagcacgacggcgctgcccagacccttgccctggtggtcgggcgagacgccgacggtggccaggaaccacgcgggctccttgggccggtgcggcgccaggaggccttccatctgttgctgcgcggccagccgggaaccgctcaactcggccatgcgcgggccgatctcggcgaacaccgcccccgcttcgacgctctccggcgtggtccagaccgccaccgcggcgccgtcgtccgcgacccacaccttgccgatgtcgagcccgacgcgcgtgaggaagagttcttgcagctcggtgacccgctcgatgtggcggtccgggtcgacggtgtggcgcgtggcggggtagtcggcgaacgcggcggcgagggtgcgtacggcccgggggacgtcgtcgcgggtggcgaggcgcaccgtgggcttgtactcggtcatgctaccgctgccgctaccgctagcagggccgggattctcctccacgtcaccgcatgttagaagacttcctctgccctcaagcttgcctgatccctttttcttttttgcctggccggcctttttcgtggccgccggccttttgtcgcctcccagctgagacaggtcgatccgtgtctcgtacaggccggtgatgctctggtggatcagggtggcgtccagcacctctttggtgctggtgtacctcttccggtcgatggtggtgtcaaagtacttgaaggcggcaggggctcccagattggtcagggtaaacaggtggatgatattctcggcctgctctctgatgggcttatcccggtgcttgttgtaggcggacagcactttgtccagattagcgtcggccaggatcactctcttggagaactcgctgatctgctcgatgatctcgtccaggtagtgcttgtgctgttccacaaacagctgtttctgctcattatcctcgggggagcccttcagcttctcatagtggctggccaggtacaggaagttcacatatttggagggcagggccagttcgtttcccttctgcagttcgccggcagaggccagcattctcttccggccgttttccagctcgaacagggagtacttaggcagcttgatgatcaggtcctttttcacttctttgtagcccttggcttccagaaagtcgatgggattcttctcgaagctgcttctttccatgatggtgatccccagcagctctttcacactcttcagtttcttggacttgcccttttccactttggccaccaccagcacagaataggccacggtggggctgtcgaagccgccgtacttcttagggtcccagtccttctttctggcgatcagcttatcgctgttcctcttgggcaggatagactctttgctgaagccgcctgtctgcacctcggtctttttcacgatattcacttggggcatgctcagcactttccgcacggtggcaaaatcccggcccttatcccacacgatctccccggtttcgccgtttgtctcgatcagaggccgcttccggatctcgccgttggccagggtaatctcggtcttgaaaaagttcatgatgttgctgtagaagaagtacttggcggtagccttgccgatttcctgctcgctcttggcgatcatcttccgcacgtcgtacaccttgtagtcgccgtacacgaactcgctttccagcttagggtactttttgatcagggcggttcccacgacggcgttcaggtaggcgtcgtgggcgtggtggtagttgttgatctcgcgcactttgtaaaactggaaatccttccggaaatcggacaccagcttggacttcagggtgatcactttcacttcccggatcagcttgtcattctcgtcgtacttagtgttcatccgggagtccaggatctgtgccacgtgctttgtgatctgccgggtttccaccagctgtctcttgatgaagccggccttatccagttcgctcaggccgcctctctcggccttggtcagattgtcgaactttctctgggtaatcagcttggcgttcagcagctgccgccagtagttcttcatcttcttcacgacctcttcggagggcacgttgtcgctcttgccccggttcttgtcgcttctggtcagcaccttgttgtcgatggagtcgtccttcagaaagctctgaggcacgatatggtccacatcgtagtcggacagccggttgatgtccagttcctggtccacgtacatatcccgcccattctgcaggtagtacaggtacagcttctcgttctgcagctgggtgttttccacggggtgttctttcaggatctggctgcccagctctttgatgccctcttcgatccgcttcattctctcgcggctgttcttctgtcccttctgggtggtctggttctctctggccatttcgatcacgatgttctcgggcttgtgccggcccatcactttcacgagctcgtccaccaccttcactgtctgcaggatgcccttcttaatggcggggctgccggccagattggcaatgtgctcgtgcaggctatcgccctggccggacacctgggctttctggatgtcctctttaaaggtcaggctgtcgtcgtggatcagctgcatgaagtttctgttggcgaagccgtcggacttcaggaaatccaggattgtcttgccggactgcttgtcccggatgccgttgatcagcttccggctcagcctgccccagccggtgtatctccgccgcttcagctgcttcatcactttgtcgtcgaacaggtgggcataggttttcagccgttcctcgatcatctctctgtcctcaaacagtgtcagggtcagcacgatatcttccagaatgtcctcgttttcctcattgtccaggaagtccttgtccttgataattttcagcagatcgtggtatgtgcccagggaggcgttgaaccgatcttccacgccggagatttccacggagtcgaagcactcgattttcttgaagtagtcctctttcagctgcttcacggtcactttccggttggtcttgaacagcaggtccacgatggcctttttctgctcgccgctcaggaaggcgggctttctcattccctcggtcacgtatttcactttggtcagctcgttatacacggtgaagtactcgtacagcaggctgtgcttgggcagcaccttctcgttgggcaggttcttatcgaagttggtcatccgctcgatgaagctctgggcggaagcgcccttgtccaccacttcctcgaagttccagggggtgatggtttcctcgctctttctggtcatccaggcgaatctgctgtttcccctggccagagggcccacgtagtaggggatgcggaaggtcaggatcttctcgatcttttcccggttgtccttcaggaatgggtaaaaatcttcctgccgccgcagaatggcgtgcagctctcccaggtggatctggtgggggatgctgccgttgtcgaaggtccgctgcttccgcagcaggtcctctctgttcagcttcacgagcagttcctcggtgccgtccatcttttccaggatgggcttgatgaacttgtagaactcttcctggctggctccgccgtcaatgtagccggcgtagccgttcttgctctggtcgaagaaaatctctttgtacttctcaggcagctgctgccgcacgagagctttcagcagggtcaggtcctggtggtgctcgtcgtatctcttgatcatagaggcgctcaggggggccttggtgatctcggtgttcactctcaggatgtcgctcagcaggatggcgtcggacaggttcttggcggccagaaacaggtcggcgtactggtcgccgatctgggccagcaggttgtccaggtcgtcgtcgtaggtgtccttgctcagctgcagtttggcatcctcggccaggtcgaagttgctcttgaagttgggggtcaggcccaggctcagggcaatcaggtttccgaacaggccattcttcttctcgccgggcagctgggcgatcagattttccagccgtctgctcttgctcagtctggcagacaggatggccttggcgtccacgccgctggcgttgatggggttttcctcgaacagctggttgtaggtctgcaccagctggatgaacagcttgtccacgtcgctgttgtcggggttcaggtcgccctcgatcaggaagtggccccggaacttgatcatgtgggccagggccagatagatcagccgcaggtcggccttgtcggtgctgtccaccagtttctttctcaggtggtagatggtggggtacttctcgtggtaggccacctcgtccacgatgttgccgaagatggggtgccgctcgtgcttcttatcctcttccaccaggaaggactcttccagtctgtggaagaagctgtcgtccaccttggccatctcgttgctgaagatctcttgcagatagcagatccggttcttccgtctggtgtatcttcttctggcggttctcttcagccgggtggcctcggctgtttcgccgctgtcgaacagcagggctccgatcaggttcttcttgatgctgtgccggtcggtgttgcccagcaccttgaatttcttgctgggcaccttgtactcgtcggtgatcacggcccagcccacagagttggtgccgatgtccaggccgatgctgtacttcttgtcggctgctgggactccgtggataccgaccttccgcttcttctttggggccatcttatcgtcatcgtctttgtaatcaatatcatgatccttgtagtctccgtcgtggtccttatagtccatgaattccaccacactggactagtaggtaccccgatccggggtctctggattagacgactgctggctgatggagcggctttgtgtcgggaggagtatccacacagcacaagaactcaaacggtagtgatatgaaaactggtcccgcagcccgttttataaagcccgcttagcgctaaaaccttatcagccgcaatgctctccgctctcaagtcgcgttcaaaacttttaccatatatgggtatgaattgttcgccgattgggttttcatttacggagaatttcctccgcaactgggtgttcgtgcttgtgtgtgtcttgtatatcgccatgaagagtgtacttccatgggtcctatcagttgcggcatgtgattcaatttgggggttactcatattcgatacttcagctggttatctttatgctgtgatggtcacggtgcttttacacattttttccccaactactcattgtatgccaaaacatacatatgtacatgtgtttatagtgggtttattagtaatgtccagcgatattaaaacattacagttaaataatattcatattagcttacgtttactgctatctgcacttcaaattggttattcagggacatttgtgcaacacacatcttctagtaggggcatcactagtcgtagaaaacacacctaacttaagccggttttaacaataacggcgaaacagctgccagacgagagaaaagtacacagcaagaagcatttctataatgatgccgtcctaaatatgcagcatttttgctcaaattgatcatttcgttgcatcgctcaggtggtatagtatttggctattgaatgtgattgctatcttaatagttttgaatagtaatagtgcaaatattcattcataaaaaataggtaatcgattttctcagtgcagtctatgcgtttgcgcaaacaggagacgaagagagagacgcctcacacacaagcacatgcggtctaagctgctctccctctttttctctctctctctgcttggcggcagttcttccagaggctgtgacgtcaggtaagctatttcgtctcggtgtgctgttagcccgtaacgataagctgtccgattcctaattctctttcaaataatttgttttatttttttttttgtgtgttttgaagtcaaacagcttcataaacaatattaaatatcgcaattgttattgcaaatgcattcgtatcaggcgccttgatgtttttcggccaaaacttcgtctgctctttgtcagcgtaattgcgtttgccggtcgtgccgtcttgctcgcacgcgcttgcaaacaaaaagtgaaagagggaaagccactgtgttgttgcttttgttgctatcgttggctgcacacacatggcaataagtaaccatattgtagacttatttattttagcgttttcgttgactaatctttgtttaaataattgcctatttcgagccaaaatgacgcataaaggacacggcaacatgcggtgcgttgagcatgcgtacgagatggcgctagagagagcgagataaaaagaaaaaaacaaccggcgagtggcgagacacgaataatacacacacgcacacatggtcaaaggagtggctccaacgtcggtgacgtcgcgcccagccacagaccccgccacgccccgccccacccttccttccgctgccccgcacccctttccttccactaccgttttgccgcatttggtattgccatctcgcttttttggctttactttgctttttgaataagccaagaaactgaaatcaaagcggacagtccgaaaattgaaactattccaaaaaacaacaaccgcacatgcactgcgttcggcaaaagacgcagaaatcagcctgtggtctggcatcatcttaaaatatcatcagtatcatcattgccattataaatacataagaaaatgagaatacattaaaggtatatacatacaatctttgtcatagtcaacatattttgcaacaaaattatctatagataacatgtttttgtagaaaattccactaactgattttcgtataaatacaaattattctcattcttcaaaatactcgccgacgtcatcgcgaaattcctcagcattcttcgacaaatgaagagagacagcgagtgacacaatcttatcttgccgacgagtctttgaaaatggccccgggtaacggtgaaaaacatatagccaagaatggagcgatcgccaggcgatggaaattggcttcaaattgctccgaatatggccagttggtcagttggcattggattgtggagagatactagatcagctcgttctccttctcctccgcattctcctactccgcccgaagacgcttctttgatcgcttctttctcgaatgtgtacatattcattatgtatttggtgtttggcgacgatcagccagctttaatggccataaaacgtgtcttggtttgtgggccacataacaggcagcgagatctgttcgacttgcagcctgaaatacggcacgagtaggaaaagccgagtcaaatgccgaatgcagagtctcattacagcacaatcaactcaagaaaaactcgacacttttttaccatttgcacttaaatccttttttattcgttatgtatactttttttggtccctaaccaaaacaaaaccaaactctcttagtcgtgcctctatatttaaaactatcaatttattatagtcaataaatcgaactgtgttttcaacaaacgaacaataggacactttgattctaaaggaaattttgaaaatcttaagcagagggttcttaagaccatttgccaattcttataattctcaactgctctttcctgatgttgatcatttatataggtatgttttcctcaatacttcggaagagcgagctcttccgttttagagctagaaatagcaagttaaaataaggctagtccgttatcaacttgaaaaagtggcaccgagtcggtgcttttttagatct)所示。在本发明的第三方面，提供了一种在昆虫细胞中进行基因编辑的方法，所述方法包括：用本发明第一方面所述的试剂处理昆虫细胞，从而进行基因编辑。在另一优选例中，所述昆虫细胞为体外培养的细胞。在另一优选例中，所述昆虫为小菜蛾、二化螟。在本发明的第四方面，提供了一种如本发明第一方面所述的试剂的用途，所述试剂用于制备试剂盒，所述试剂盒用于：(a)敲除abc转运蛋白基因；和/或(b)用于编辑昆虫细胞基因。在本发明的第五方面，提供了一种sgrna，所述sgrna靶向abc转运蛋白基因的sgrna。在另一优选例中，所述sgrna的识别区序列具有seqidno.:1所示的核苷酸序列。在本发明的第六方面，提供了一种载体，所述载体包含本发明第五方面所述的sgrna。在本发明的第七方面，提供了一种基因编辑系统，所述基因编辑系统包含如本发明第五方面所述的sgrna。在另一优选例中，所述基因编辑系统为crispr/cas系统，优选地为crispr/cas9系统。在另一优选例中，利用所述crispr/cas9系统进行基因失活编辑，获得的基因失活率≥70％，较佳地≥80％，更佳地≥90％。在本发明的第八方面，提供了一种杀虫方法，所述方法包括：用本发明第一方面所述的试剂处理昆虫细胞，从而进行基因编辑。在另一优选例中，所述昆虫细胞为体外培养的细胞。在另一优选例中，所述昆虫为小菜蛾、二化螟。应理解，在本发明范围内中，本发明的上述各技术特征和在下文(如实施例)中具体描述的各技术特征之间都可以互相组合，从而构成新的或优选的技术方案。限于篇幅，在此不再一一累述。附图说明图1显示了本发明所用载体结构示意图。图2显示了pac-cas9-abch1.exon2-grna质粒测序结果。图3显示了pac-cas9-abch1.exon13-grna质粒测序结果。图4显示了crispr/cas9的工作原理及grna设计规则。具体实施方式本发明人经过广泛而深入的研究，首次开发了一种适用于昆虫细胞基因编辑的crispr/cas9载体构建方法及应用其方法构建的质粒。应用所述方法构建的靶基因abch1的编辑质粒能够转染昆虫细胞，利用crispr工作机制对基因组dna进行精确编辑，以期获得理想的表型，可以施用于农业害虫控制，用以替代化学农药。在此基础上完成了本发明。术语除非另外定义，本文使用的所有技术和科学术语的意义与本发明所属领域普通技术人员通常所理解的相同。本文中述及的所有出版物和其他参考文献都通过引用纳入本文。如本文所用，所述的“含有”，“具有”或“包括”包括了“包含”、“主要由……构成”、“基本上由……构成”、和“由……构成”。crispr/cas基因编辑技术本发明所称的“crispr/cas技术”、“crispr/cas基因组编辑”、“crispr/cas基因组编辑技术”、“crispr/cas基因组编辑方法”一般是指利用crispr/cas系统对目的dna序列进行修改的技术。“crispr/cas技术”也可以包含利用类似原理进行基因表达调控的方法，如基于crispr/dcas9的基因表达调控技术。crispr(clusteredregularlyinterspacedshortpalindromicrepeats)，全称为规律成簇间隔短回文重复，是近年来发展迅速的基因编辑工具，起初被发现是细菌用以保护自身对抗病毒感染的获得性免疫系统应答现象，是一种对付攻击者的基因武器。后来，研究人员发现，它可以经过改造和发展成为精确而高效的基因编辑工具，可以介导基因组序列的删除、插入、突变，甚至还可以激活或抑制目标基因，这些目标基因包括人、鼠、斑马鱼、细菌、果蝇、酵母、线虫和农作物细胞内的基因，意味着crispr/cas9是一种可以广泛使用的基因编辑工具。目前，来自streptococcuspyogenes的crispr-cas9系统应用最为广泛。该系统中的核酸酶cas9蛋白含有两个核酸酶结构域，可以分别切割dna两条单链。crispr/cas9系统的工作原理是cas9首先与crrna及tracrrna结合成复合物，然后通过pam序列结合特定dna序列，形成rna-dna复合结构，进而对目的dna双链进行切割，使dna双链断裂，随后机体通过启动非同源末端链接或者同源重组机制对出现的双链断裂进行修复，继而介导核苷酸序列的缺失、插入等。本发明的sgrna如本文所用，“sgrna”、“向导rna”或“本发明sgrna”可互换使用，均指靶向abc转运蛋白基因的sgrna，所述sgrna具有seqidno.:1所示的核苷酸序列：gtcgccgccgggcgacgaga。cas9蛋白cas9蛋白为多功能蛋白，其蛋白结构包括α-螺旋组成的识别区(rec)、由hnh结构域与ruvc结构域组成的核酸酶区以及位于c-端的pam结合区。这两个重要的核酸酶结构域ruvc与hnh可分别对grna的dna互补链与非互补链进行切割，产生平末端的dna双链断裂。当ruvc结构域中的d10a发生突变时可导致ruvc结构域的失活，当hnh结构域中的h840a发生突变时则可导致hnh结构域的失活。单点突变体可使cas9成为切口酶(nickase)，简称为ncas9，可形成单链dna断裂。cas9与靶dna的识别依赖于tracrrna:crrna复合体以及位于靶位点下游的pam序列。本发明的sgrna介导的crispr/cas系统在本发明的sgrna的基础上，本发明提供包含所述的sgrna的基因编辑系统，优选地为crispr/cas系统。本领域技术人员知晓，crispr/cas系统可以用于各种领域，包括但不限于基因组编辑、基因表达调控与基因工程等等。在具体的实施方式中，本发明的crispr/cas系统用于基因组编辑和基因表达调控。在具体的实施方式中，所述crispr/cas系统包括但不限于crispr/cas9系统、crispr/ncas9系统或crispr/dcas9系统；优选地，所述crispr/cas系统是crispr/cas9系统。如上所述，本发明的crispr/cas系统的核心是cas9蛋白以及sgrna，二者可以在同一表达载体中也可以在不同的表达载体中。然而，由于sgrna和cas9蛋白在不同表达载体中减少了sgrna表达盒亚克隆至cas9质粒的过程，使得操作更为简便，因此，在优选的实施方式中，将sgrna的表达盒与cas9的表达质粒共转化。基于本发明的技术内容以及本领域的技术常识，本领域技术人员知晓关于cas9的表达、靶向序列的选择以及dna转化系统的各种技术要点，例如本领域技术人员可以参照nodvig等(nodvigetal.,2015)所述进行cas9的表达以及实施dna转化。在本文中，术语“基因失活”是指在不涉及供体dna的基础上，由cas9在sgrna的引导下对特定位点进行双链切割后，在真核生物中由非同源末端连接系统引入dna序列插入或碱基缺失等而使得某一基因丧失生物学功能；而术语“基因精准编辑”是指在特定dna位点进行精准的、可预测的遗传学操作，例如目标dna片段的敲除、插入、替换、点突变等等，涉及供体dna以及同源重组机制。因此，本领域技术人员知晓，基因精准编辑的难度要远高于基因失活。此外，本领域技术人员知晓，供体dna片段供体dna的长度对于基因组编辑的效率也有很大影响。供体dna片段供体dna同源臂长度增加可提高dna同源重组效率从而提高dna编辑效率，但多数真核野生菌株中其同源重组的效率很低，有时仅有百分之几的效率，主要是非同源末端连接系统发挥作用。而对于短同源臂的供体dna片段供体dna片段，其基因组编辑效率则更低。然而，本发明的sgrna介导的基因组编辑系统相比于现有技术具备优异的基因组编辑能力。在具体的实施方式例中，利用本发明的基因组编辑系统获得的基因失活率高于95％，更优选达到100％。本发明的主要优点包括：1)本发明利用crispr/cas9方法精确失活abch1蛋白以精准控制某种害虫的种群数量，避免了伤害其他有益昆虫；2)本发明的方法避免了使用化学农药，保护了环境；3)本发明的方法产生的害虫不易产生耐药性。4)本发明的方法避免了使用化学农药，保障了食品安全。下面结合具体实施例，进一步阐述本发明。应理解，这些实施例仅用于说明本发明而不用于限制本发明的范围。下列实施例中未注明具体条件的实验方法，通常按照常规条件，例如sambrook等人，分子克隆：实验室手册(newyork:coldspringharborlaboratorypress,1989)中所述的条件，或按照制造厂商所建议的条件。除非另外说明，否则百分比和份数是重量百分比和重量份数。通用方法1)特定基因的grna设计crispr/cas9方法是针对基因组dna编辑而开发的方案，故grna设计须以dna为对象，设计过程见网址http://crispr.mit.edu/。grna载体制作由正向序列与反向序列退火而成。设计好的寡核苷酸进行dna合成。2)变性及复性。以上寡核苷酸在合成之后，加水溶解成100um。对于每条grna，正向序列与反向序列各加10ul，并加ddh2o2oul，混匀后加热至98℃，自然冷却至室温。3)磷酸化oligo。对于每条grna，取步骤2)退火产物1ul，t4多聚核苷酸激酶(neb)1ul，t4dna连接酶缓冲液1ul，混匀后37℃孵育30min，完成后加ddh2o9oul至100ul。4)载体酶切。配置反应体系：pac-sgrna-cas9载体2ug，bspqi(neb,10u/ul)内切酶1ul，10×nebuffer内切酶缓冲液5ul，添加ddh2o至总体积50ul。50℃孵育2小时，然后80℃孵育20分钟进行热失活，胶回收载体片段。5)连接。对于每条grna：在0.2ml离心管中添加上述步骤3)中所述磷酸化产物2ul，添加上述4)中酶切载体产物50ng，t4dna连接酶1ul，及t4dna连接酶缓冲液至总体积10ul，混匀后18℃孵育2小时。6)转化。对于每条grna，把上述步骤5)连接产物转化dh5α感受态细胞，涂布在含氨苄青霉素(ampr)的lb抗性平板上，37℃培养过夜，挑选单菌落，加入lb培养基37℃培养6小时，提取重组质粒。7)鉴定。对于每条grna，用u6fwd引物(cgagatctgttcgacttgcagcc)测序鉴定重组质粒。测序结果必须质量过关，结果可靠。实施例1.本实施例以农业害虫小菜蛾为例进行具体说明。1)abch1基因grna设计为突变abch1基因，破坏其蛋白结构，grna只能在各个外显子中寻找。上述abch1基因为含有13个外显子的及长内含子的基因，其13个外显子序列见seqidno.11-25(表2)。本发明选择第2个与第13个外显子进行grna设计，序列为seqidno.5-8(表1)，该寡核苷酸由生工生物公司合成。2)变性及复性。以上寡核苷酸在合成之后，加水溶解成100um。对于exon2，exon2f与exon2r各加10ul，并加ddh2o2oul，混匀后加热至98℃，自然冷却至室温。对于exon13，exon13f与exon13r各加10ul，并加ddh2o2oul，混匀后加热至98℃，自然冷却至室温。3)磷酸化oligo。exon2退火产物1ul，t4多聚核苷酸激酶(neb)1ul，t4dna连接酶缓冲液1ul，混匀后37℃孵育30min，完成后加ddh2o9oul至100ul。exon13退火产物1ul，t4多聚核苷酸激酶(neb)1ul，t4dna连接酶缓冲液1ul，混匀后37℃孵育30min，完成后加ddh2o9oul至100ul。4)载体酶切。所述载体结构示意图见图1，序列见seqidno.5。配置反应体系：pac-sgrna-cas9载体2ug，bspqi(neb,10u/ul)内切酶1ul，10xnebuffer内切酶缓冲液5ul，添加ddh2o至总体积50ul。50℃孵育2小时，然后80℃孵育20分钟进行热失活，胶回收载体片段。5)连接。对于exon2：在0.2ml离心管中添加上述步骤3)中exon2产物2ul，添加上述4)步产物50ng，t4dna连接酶1ul，及t4dna连接酶缓冲液至10ul总体积，混匀后18℃孵育2小时。对于exon13：在0.2ml离心管中添加上述步骤3)中exon13产物2ul，添加上述4)步产物50ng，t4dna连接酶1ul，及t4dna连接酶缓冲液至10ul总体积，混匀后18℃孵育2小时。6)转化。把上述步骤5)连接产物转化dh5α感受态细胞，涂布在含氨苄青霉素(ampr)的lb抗性平板上，37℃培养过夜，挑选单菌落，加入lb培养基37℃培养6小时，提取重组质粒。7)鉴定。用u6fwd引物(cgagatctgttcgacttgcagcc)测序鉴定重组质粒。对于pac-cas9-abch1.exon2-grna质粒，测序结果峰图见图2。对于pac-cas9-abch1.exon2-grna质粒，测序结果峰图见图3。上述两载体测序质量均很高，结果可靠。表1序列seqidno.:exon2fttcggtcgccgccgggcgacgaga5exon2raactctcgtcgcccggcggcgacc6exon13fttcggacttgcgactacaacccga7exon13raactcgggttgtagtcgcaagtcc8pac-cas9-abch1.exon2-grna(seqidno.:9：agatctaaaaaagcaccgactcggtgccactttttcaagttgataacggactagccttattttaacttgctatttctagctctaaaactctcgtcgcccggcggcgaccgaagtattgaggaaaacatacctatataaatgatcaacatcaggaaagagcagttgagaattataagaattggcaaatggtcttaagaaccctctgcttaagattttcaaaatttcctttagaatcaaagtgtcctattgttcgtttgttgaaaacacagttcgatttattgactataataaattgatagttttaaatatagaggcacgactaagagagtttggttttgttttggttagggaccaaaaaaagtatacataacgaataaaaaaggatttaagtgcaaatggtaaaaaagtgtcgagtttttcttgagttgattgtgctgtaatgagactctgcattcggcatttgactcggcttttcctactcgtgccgtatttcaggctgcaagtcgaacagatctcgctgcctgttatgtggcccacaaaccaagacacgttttatggccattaaagctggctgatcgtcgccaaacaccaaatacataatgaatatgtacacattcgagaaagaagcgatcaaagaagcgtcttcgggcggagtaggagaatgcggaggagaaggagaacgagctgatctagtatctctccacaatccaatgccaactgaccaactggccatattcggagcaatttgaagccaatttccatcgcctggcgatcgctccattcttggctatatgtttttcaccgttacccggggccattttcaaagactcgtcggcaagataagattgtgtcactcgctgtctctcttcatttgtcgaagaatgctgaggaatttcgcgatgacgtcggcgagtattttgaagaatgagaataatttgtatttatacgaaaatcagttagtggaattttctacaaaaacatgttatctatagataattttgttgcaaaatatgttgactatgacaaagattgtatgtatatacctttaatgtattctcattttcttatgtatttataatggcaatgatgatactgatgatattttaagatgatgccagaccacaggctgatttctgcgtcttttgccgaacgcagtgcatgtgcggttgttgttttttggaatagtttcaattttcggactgtccgctttgatttcagtttcttggcttattcaaaaagcaaagtaaagccaaaaaagcgagatggcaataccaaatgcggcaaaacggtagtggaaggaaaggggtgcggggcagcggaaggaagggtggggcggggcgtggcggggtctgtggctgggcgcgacgtcaccgacgttggagccactcctttgaccatgtgtgcgtgtgtgtattattcgtgtctcgccactcgccggttgtttttttctttttatctcgctctctctagcgccatctcgtacgcatgctcaacgcaccgcatgttgccgtgtcctttatgcgtcattttggctcgaaataggcaattatttaaacaaagattagtcaacgaaaacgctaaaataaataagtctacaatatggttacttattgccatgtgtgtgcagccaacgatagcaacaaaagcaacaacacagtggctttccctctttcactttttgtttgcaagcgcgtgcgagcaagacggcacgaccggcaaacgcaattacgctgacaaagagcagacgaagttttggccgaaaaacatcaaggcgcctgatacgaatgcatttgcaataacaattgcgatatttaatattgtttatgaagctgtttgacttcaaaacacacaaaaaaaaaaataaaacaaattatttgaaagagaattaggaatcggacagcttatcgttacgggctaacagcacaccgagacgaaatagcttacctgacgtcacagcctctggaagaactgccgccaagcagagagagagagaaaaagagggagagcagcttagaccgcatgtgcttgtgtgtgaggcgtctctctcttcgtctcctgtttgcgcaaacgcatagactgcactgagaaaatcgattacctattttttatgaatgaatatttgcactattactattcaaaactattaagatagcaatcacattcaatagccaaatactataccacctgagcgatgcaacgaaatgatcaatttgagcaaaaatgctgcatatttaggacggcatcattatagaaatgcttcttgctgtgtacttttctctcgtctggcagctgtttcgccgttattgttaaaaccggcttaagttaggtgtgttttctacgactagtgatgcccctactagaagatgtgtgttgcacaaatgtccctgaataaccaatttgaagtgcagatagcagtaaacgtaagctaatatgaatattatttaactgtaatgttttaatatcgctggacattactaataaacccactataaacacatgtacatatgtatgttttggcatacaatgagtagttggggaaaaaatgtgtaaaagcaccgtgaccatcacagcataaagataaccagctgaagtatcgaatatgagtaacccccaaattgaatcacatgccgcaactgataggacccatggaagtacactcttcatggcgatatacaagacacacacaagcacgaacacccagttgcggaggaaattctccgtaaatgaaaacccaatcggcgaacaattcatacccatatatggtaaaagttttgaacgcgacttgagagcggagagcattgcggctgataaggttttagcgctaagcgggctttataaaacgggctgcgggaccagttttcatatcactaccgtttgagttcttgtgctgtgtggatactcctcccgacacaaagccgctccatcagccagcagtcgtctaatccagagaccccggatcggggtacctactagtccagtgtggtggaattcatggactataaggaccacgacggagactacaaggatcatgatattgattacaaagacgatgacgataagatggccccaaagaagaagcggaaggtcggtatccacggagtcccagcagccgacaagaagtacagcatcggcctggacatcggcaccaactctgtgggctgggccgtgatcaccgacgagtacaaggtgcccagcaagaaattcaaggtgctgggcaacaccgaccggcacagcatcaagaagaacctgatcggagccctgctgttcgacagcggcgaaacagccgaggccacccggctgaagagaaccgccagaagaagatacaccagacggaagaaccggatctgctatctgcaagagatcttcagcaacgagatggccaaggtggacgacagcttcttccacagactggaagagtccttcctggtggaagaggataagaagcacgagcggcaccccatcttcggcaacatcgtggacgaggtggcctaccacgagaagtaccccaccatctaccacctgagaaagaaactggtggacagcaccgacaaggccgacctgcggctgatctatctggccctggcccacatgatcaagttccggggccacttcctgatcgagggcgacctgaaccccgacaacagcgacgtggacaagctgttcatccagctggtgcagacctacaaccagctgttcgaggaaaaccccatcaacgccagcggcgtggacgccaaggccatcctgtctgccagactgagcaagagcagacggctggaaaatctgatcgcccagctgcccggcgagaagaagaatggcctgttcggaaacctgattgccctgagcctgggcctgacccccaacttcaagagcaacttcgacctggccgaggatgccaaactgcagctgagcaaggacacctacgacgacgacctggacaacctgctggcccagatcggcgaccagtacgccgacctgtttctggccgccaagaacctgtccgacgccatcctgctgagcgacatcctgagagtgaacaccgagatcaccaaggcccccctgagcgcctctatgatcaagagatacgacgagcaccaccaggacctgaccctgctgaaagctctcgtgcggcagcagctgcctgagaagtacaaagagattttcttcgaccagagcaagaacggctacgccggctacattgacggcggagccagccaggaagagttctacaagttcatcaagcccatcctggaaaagatggacggcaccgaggaactgctcgtgaagctgaacagagaggacctgctgcggaagcagcggaccttcgacaacggcagcatcccccaccagatccacctgggagagctgcacgccattctgcggcggcaggaagatttttacccattcctgaaggacaaccgggaaaagatcgagaagatcctgaccttccgcatcccctactacgtgggccctctggccaggggaaacagcagattcgcctggatgaccagaaagagcgaggaaaccatcaccccctggaacttcgaggaagtggtggacaagggcgcttccgcccagagcttcatcgagcggatgaccaacttcgataagaacctgcccaacgagaaggtgctgcccaagcacagcctgctgtacgagtacttcaccgtgtataacgagctgaccaaagtgaaatacgtgaccgagggaatgagaaagcccgccttcctgagcggcgagcagaaaaaggccatcgtggacctgctgttcaagaccaaccggaaagtgaccgtgaagcagctgaaagaggactacttcaagaaaatcgagtgcttcgactccgtggaaatctccggcgtggaagatcggttcaacgcctccctgggcacataccacgatctgctgaaaattatcaaggacaaggacttcctggacaatgaggaaaacgaggacattctggaagatatcgtgctgaccctgacactgtttgaggacagagagatgatcgaggaacggctgaaaacctatgcccacctgttcgacgacaaagtgatgaagcagctgaagcggcggagatacaccggctggggcaggctgagccggaagctgatcaacggcatccgggacaagcagtccggcaagacaatcctggatttcctgaagtccgacggcttcgccaacagaaacttcatgcagctgatccacgacgacagcctgacctttaaagaggacatccagaaagcccaggtgtccggccagggcgatagcctgcacgagcacattgccaatctggccggcagccccgccattaagaagggcatcctgcagacagtgaaggtggtggacgagctcgtgaaagtgatgggccggcacaagcccgagaacatcgtgatcgaaatggccagagagaaccagaccacccagaagggacagaagaacagccgcgagagaatgaagcggatcgaagagggcatcaaagagctgggcagccagatcctgaaagaacaccccgtggaaaacacccagctgcagaacgagaagctgtacctgtactacctgcagaatgggcgggatatgtacgtggaccaggaactggacatcaaccggctgtccgactacgatgtggaccatatcgtgcctcagagctttctgaaggacgactccatcgacaacaaggtgctgaccagaagcgacaagaaccggggcaagagcgacaacgtgccctccgaagaggtcgtgaagaagatgaagaactactggcggcagctgctgaacgccaagctgattacccagagaaagttcgacaatctgaccaaggccgagagaggcggcctgagcgaactggataaggccggcttcatcaagagacagctggtggaaacccggcagatcacaaagcacgtggcacagatcctggactcccggatgaacactaagtacgacgagaatgacaagctgatccgggaagtgaaagtgatcaccctgaagtccaagctggtgtccgatttccggaaggatttccagttttacaaagtgcgcgagatcaacaactaccaccacgcccacgacgcctacctgaacgccgtcgtgggaaccgccctgatcaaaaagtaccctaagctggaaagcgagttcgtgtacggcgactacaaggtgtacgacgtgcggaagatgatcgccaagagcgagcaggaaatcggcaaggctaccgccaagtacttcttctacagcaacatcatgaactttttcaagaccgagattaccctggccaacggcgagatccggaagcggcctctgatcgagacaaacggcgaaaccggggagatcgtgtgggataagggccgggattttgccaccgtgcggaaagtgctgagcatgccccaagtgaatatcgtgaaaaagaccgaggtgcagacaggcggcttcagcaaagagtctatcctgcccaagaggaacagcgataagctgatcgccagaaagaaggactgggaccctaagaagtacggcggcttcgacagccccaccgtggcctattctgtgctggtggtggccaaagtggaaaagggcaagtccaagaaactgaagagtgtgaaagagctgctggggatcaccatcatggaaagaagcagcttcgagaagaatcccatcgactttctggaagccaagggctacaaagaagtgaaaaaggacctgatcatcaagctgcctaagtactccctgttcgagctggaaaacggccggaagagaatgctggcctctgccggcgaactgcagaagggaaacgaactggccctgccctccaaatatgtgaacttcctgtacctggccagccactatgagaagctgaagggctcccccgaggataatgagcagaaacagctgtttgtggaacagcacaagcactacctggacgagatcatcgagcagatcagcgagttctccaagagagtgatcctggccgacgctaatctggacaaagtgctgtccgcctacaacaagcaccgggataagcccatcagagagcaggccgagaatatcatccacctgtttaccctgaccaatctgggagcccctgccgccttcaagtactttgacaccaccatcgaccggaagaggtacaccagcaccaaagaggtgctggacgccaccctgatccaccagagcatcaccggcctgtacgagacacggatcgacctgtctcagctgggaggcgacaaaaggccggcggccacgaaaaaggccggccaggcaaaaaagaaaaagggatcaggcaagcttgagggcagaggaagtcttctaacatgcggtgacgtggaggagaatcccggccctgctagcggtagcggcagcggtagcatgaccgagtacaagcccacggtgcgcctcgccacccgcgacgacgtcccccgggccgtacgcaccctcgccgccgcgttcgccgactaccccgccacgcgccacaccgtcgacccggaccgccacatcgagcgggtcaccgagctgcaagaactcttcctcacgcgcgtcgggctcgacatcggcaaggtgtgggtcgcggacgacggcgccgcggtggcggtctggaccacgccggagagcgtcgaagcgggggcggtgttcgccgagatcggcccgcgcatggccgagttgagcggttcccggctggccgcgcagcaacagatggaaggcctcctggcgccgcaccggcccaaggagcccgcgtggttcctggccaccgtcggcgtctcgcccgaccaccagggcaagggtctgggcagcgccgtcgtgctccccggagtggaggcggccgagcgcgccggggtgcccgccttcctggagacctccgcgccccgcaacctccccttctacgagcggctcggcttcaccgtcaccgccgacgtcgaggtgcccgaaggaccgcgcacctggtgcatgacccgcaagcccggtgcctgactcgagggatccagacatgataagatacattgatgagtttggacaaaccacaactagaatgcagtgaaaaaaatgctttatttgtgaaatttgtgatgctattgctttatttgtaaccattataagctgcaataaacaagttaacaacaacaattgcattcattttatgtttcaggttcagggggaggtgtgggaggttttttaaagcaagtaaaacctctacaaatgtggtatggctgattatgatcagtcgaccgatgcccttgagagccttcaacccagtcagctccttccggtgggcgcggggcatgactatcgtcgccgcacttatgactgtcttctttatcatgcaactcgtaggacaggtgccggcagcgctctgggtcattttcggcgaggaccgctttcgctggagcgcgacgatgatcggcctgtcgcttgcggtattcggaatcttgcacgccctcgctcaagccttcgtcactggtcccgccaccaaacgtttcggcgagaagcaggccattatcgccggcatggcggccgacgcgctgggctacgtcttgctggcgttcgcgacgcgaggctggatggccttccccattatgattcttctcgcttccggcggcatcgggatgcccgcgttgcaggccatgctgtccaggcaggtagatgacgaccatcagggacagcttcaaggatcgctcgcggctcttaccagccagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgtttttccataggctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccccctggaagctccctcgtgcgctctcctgttccgaccctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcaatgctcacgctgtaggtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccggtaactatcgtcttgagtccaacccggtaagacacgacttatcgccactggcagcagccactggtaacaggattagcagagcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacggctacactagaaggacagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttctacggggtctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaaggatcttcacctagatccttttaaattaaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtctgacagttaccaatgcttaatcagtgaggcacctatctcagcgatctgtctatttcgttcatccatagttgcctgactccccgtcgtgtagataactacgatacgggagggcttaccatctggccccagtgctgcaatgataccgcgagacccacgctcaccggctccagatttatcagcaataaaccagccagccggaagggccgagcgcagaagtggtcctgcaactttatccgcctccatccagtctattaattgttgccgggaagctagagtaagtagttcgccagttaatagtttgcgcaacgttgttgccattgctgcaggcatcgtggtgtcacgctcgtcgtttggtatggcttcattcagctccggttcccaacgatcaaggcgagttacatgatcccccatgttgtgcaaaaaagcggttagctccttcggtcctccgatcgttgtcagaagtaagttggccgcagtgttatcactcatggttatggcagcactgcataattctcttactgtcatgccatccgtaagatgcttttctgtgactggtgagtactcaaccaagtcattctgagaatagtgtatgcggcgaccgagttgctcttgcccggcgtcaacacgggataataccgcgccacatagcagaactttaaaagtgctcatcattggaaaacgttcttcggggcgaaaactctcaaggatcttaccgctgttgagatccagttcgatgtaacccactcgtgcacccaactgatcttcagcatcttttactttcaccagcgtttctgggtgagcaaaaacaggaaggcaaaatgccgcaaaaaagggaataagggcgacacggaaatgttgaatactcatactcttcctttttcaatattattgaagcatttatcagggttattgtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaataggggttccgcgcacatttccccgaaaagtgccacctgacgtctaagaaaccattattatcatgacattaacctataaaaataggcgtatcacgaggccctttcgtcttcaaggaattaattctatattctaaaaacacaaatgatacttctaaaaaaaatcatgaatggcatcaactctgaatcaaatctttgcagatgcacctacttctcatttccactgtcacatcatttttcc)pac-cas9-abch1.exon13-grna(seqidno.:10：agatctaaaaaagcaccgactcggtgccactttttcaagttgataacggactagccttattttaacttgctatttctagctctaaaactcgggttgtagtcgcaagtccgaagtattgaggaaaacatacctatataaatgatcaacatcaggaaagagcagttgagaattataagaattggcaaatggtcttaagaaccctctgcttaagattttcaaaatttcctttagaatcaaagtgtcctattgttcgtttgttgaaaacacagttcgatttattgactataataaattgatagttttaaatatagaggcacgactaagagagtttggttttgttttggttagggaccaaaaaaagtatacataacgaataaaaaaggatttaagtgcaaatggtaaaaaagtgtcgagtttttcttgagttgattgtgctgtaatgagactctgcattcggcatttgactcggcttttcctactcgtgccgtatttcaggctgcaagtcgaacagatctcgctgcctgttatgtggcccacaaaccaagacacgttttatggccattaaagctggctgatcgtcgccaaacaccaaatacataatgaatatgtacacattcgagaaagaagcgatcaaagaagcgtcttcgggcggagtaggagaatgcggaggagaaggagaacgagctgatctagtatctctccacaatccaatgccaactgaccaactggccatattcggagcaatttgaagccaatttccatcgcctggcgatcgctccattcttggctatatgtttttcaccgttacccggggccattttcaaagactcgtcggcaagataagattgtgtcactcgctgtctctcttcatttgtcgaagaatgctgaggaatttcgcgatgacgtcggcgagtattttgaagaatgagaataatttgtatttatacgaaaatcagttagtggaattttctacaaaaacatgttatctatagataattttgttgcaaaatatgttgactatgacaaagattgtatgtatatacctttaatgtattctcattttcttatgtatttataatggcaatgatgatactgatgatattttaagatgatgccagaccacaggctgatttctgcgtcttttgccgaacgcagtgcatgtgcggttgttgttttttggaatagtttcaattttcggactgtccgctttgatttcagtttcttggcttattcaaaaagcaaagtaaagccaaaaaagcgagatggcaataccaaatgcggcaaaacggtagtggaaggaaaggggtgcggggcagcggaaggaagggtggggcggggcgtggcggggtctgtggctgggcgcgacgtcaccgacgttggagccactcctttgaccatgtgtgcgtgtgtgtattattcgtgtctcgccactcgccggttgtttttttctttttatctcgctctctctagcgccatctcgtacgcatgctcaacgcaccgcatgttgccgtgtcctttatgcgtcattttggctcgaaataggcaattatttaaacaaagattagtcaacgaaaacgctaaaataaataagtctacaatatggttacttattgccatgtgtgtgcagccaacgatagcaacaaaagcaacaacacagtggctttccctctttcactttttgtttgcaagcgcgtgcgagcaagacggcacgaccggcaaacgcaattacgctgacaaagagcagacgaagttttggccgaaaaacatcaaggcgcctgatacgaatgcatttgcaataacaattgcgatatttaatattgtttatgaagctgtttgacttcaaaacacacaaaaaaaaaaataaaacaaattatttgaaagagaattaggaatcggacagcttatcgttacgggctaacagcacaccgagacgaaatagcttacctgacgtcacagcctctggaagaactgccgccaagcagagagagagagaaaaagagggagagcagcttagaccgcatgtgcttgtgtgtgaggcgtctctctcttcgtctcctgtttgcgcaaacgcatagactgcactgagaaaatcgattacctattttttatgaatgaatatttgcactattactattcaaaactattaagatagcaatcacattcaatagccaaatactataccacctgagcgatgcaacgaaatgatcaatttgagcaaaaatgctgcatatttaggacggcatcattatagaaatgcttcttgctgtgtacttttctctcgtctggcagctgtttcgccgttattgttaaaaccggcttaagttaggtgtgttttctacgactagtgatgcccctactagaagatgtgtgttgcacaaatgtccctgaataaccaatttgaagtgcagatagcagtaaacgtaagctaatatgaatattatttaactgtaatgttttaatatcgctggacattactaataaacccactataaacacatgtacatatgtatgttttggcatacaatgagtagttggggaaaaaatgtgtaaaagcaccgtgaccatcacagcataaagataaccagctgaagtatcgaatatgagtaacccccaaattgaatcacatgccgcaactgataggacccatggaagtacactcttcatggcgatatacaagacacacacaagcacgaacacccagttgcggaggaaattctccgtaaatgaaaacccaatcggcgaacaattcatacccatatatggtaaaagttttgaacgcgacttgagagcggagagcattgcggctgataaggttttagcgctaagcgggctttataaaacgggctgcgggaccagttttcatatcactaccgtttgagttcttgtgctgtgtggatactcctcccgacacaaagccgctccatcagccagcagtcgtctaatccagagaccccggatcggggtacctactagtccagtgtggtggaattcatggactataaggaccacgacggagactacaaggatcatgatattgattacaaagacgatgacgataagatggccccaaagaagaagcggaaggtcggtatccacggagtcccagcagccgacaagaagtacagcatcggcctggacatcggcaccaactctgtgggctgggccgtgatcaccgacgagtacaaggtgcccagcaagaaattcaaggtgctgggcaacaccgaccggcacagcatcaagaagaacctgatcggagccctgctgttcgacagcggcgaaacagccgaggccacccggctgaagagaaccgccagaagaagatacaccagacggaagaaccggatctgctatctgcaagagatcttcagcaacgagatggccaaggtggacgacagcttcttccacagactggaagagtccttcctggtggaagaggataagaagcacgagcggcaccccatcttcggcaacatcgtggacgaggtggcctaccacgagaagtaccccaccatctaccacctgagaaagaaactggtggacagcaccgacaaggccgacctgcggctgatctatctggccctggcccacatgatcaagttccggggccacttcctgatcgagggcgacctgaaccccgacaacagcgacgtggacaagctgttcatccagctggtgcagacctacaaccagctgttcgaggaaaaccccatcaacgccagcggcgtggacgccaaggccatcctgtctgccagactgagcaagagcagacggctggaaaatctgatcgcccagctgcccggcgagaagaagaatggcctgttcggaaacctgattgccctgagcctgggcctgacccccaacttcaagagcaacttcgacctggccgaggatgccaaactgcagctgagcaaggacacctacgacgacgacctggacaacctgctggcccagatcggcgaccagtacgccgacctgtttctggccgccaagaacctgtccgacgccatcctgctgagcgacatcctgagagtgaacaccgagatcaccaaggcccccctgagcgcctctatgatcaagagatacgacgagcaccaccaggacctgaccctgctgaaagctctcgtgcggcagcagctgcctgagaagtacaaagagattttcttcgaccagagcaagaacggctacgccggctacattgacggcggagccagccaggaagagttctacaagttcatcaagcccatcctggaaaagatggacggcaccgaggaactgctcgtgaagctgaacagagaggacctgctgcggaagcagcggaccttcgacaacggcagcatcccccaccagatccacctgggagagctgcacgccattctgcggcggcaggaagatttttacccattcctgaaggacaaccgggaaaagatcgagaagatcctgaccttccgcatcccctactacgtgggccctctggccaggggaaacagcagattcgcctggatgaccagaaagagcgaggaaaccatcaccccctggaacttcgaggaagtggtggacaagggcgcttccgcccagagcttcatcgagcggatgaccaacttcgataagaacctgcccaacgagaaggtgctgcccaagcacagcctgctgtacgagtacttcaccgtgtataacgagctgaccaaagtgaaatacgtgaccgagggaatgagaaagcccgccttcctgagcggcgagcagaaaaaggccatcgtggacctgctgttcaagaccaaccggaaagtgaccgtgaagcagctgaaagaggactacttcaagaaaatcgagtgcttcgactccgtggaaatctccggcgtggaagatcggttcaacgcctccctgggcacataccacgatctgctgaaaattatcaaggacaaggacttcctggacaatgaggaaaacgaggacattctggaagatatcgtgctgaccctgacactgtttgaggacagagagatgatcgaggaacggctgaaaacctatgcccacctgttcgacgacaaagtgatgaagcagctgaagcggcggagatacaccggctggggcaggctgagccggaagctgatcaacggcatccgggacaagcagtccggcaagacaatcctggatttcctgaagtccgacggcttcgccaacagaaacttcatgcagctgatccacgacgacagcctgacctttaaagaggacatccagaaagcccaggtgtccggccagggcgatagcctgcacgagcacattgccaatctggccggcagccccgccattaagaagggcatcctgcagacagtgaaggtggtggacgagctcgtgaaagtgatgggccggcacaagcccgagaacatcgtgatcgaaatggccagagagaaccagaccacccagaagggacagaagaacagccgcgagagaatgaagcggatcgaagagggcatcaaagagctgggcagccagatcctgaaagaacaccccgtggaaaacacccagctgcagaacgagaagctgtacctgtactacctgcagaatgggcgggatatgtacgtggaccaggaactggacatcaaccggctgtccgactacgatgtggaccatatcgtgcctcagagctttctgaaggacgactccatcgacaacaaggtgctgaccagaagcgacaagaaccggggcaagagcgacaacgtgccctccgaagaggtcgtgaagaagatgaagaactactggcggcagctgctgaacgccaagctgattacccagagaaagttcgacaatctgaccaaggccgagagaggcggcctgagcgaactggataaggccggcttcatcaagagacagctggtggaaacccggcagatcacaaagcacgtggcacagatcctggactcccggatgaacactaagtacgacgagaatgacaagctgatccgggaagtgaaagtgatcaccctgaagtccaagctggtgtccgatttccggaaggatttccagttttacaaagtgcgcgagatcaacaactaccaccacgcccacgacgcctacctgaacgccgtcgtgggaaccgccctgatcaaaaagtaccctaagctggaaagcgagttcgtgtacggcgactacaaggtgtacgacgtgcggaagatgatcgccaagagcgagcaggaaatcggcaaggctaccgccaagtacttcttctacagcaacatcatgaactttttcaagaccgagattaccctggccaacggcgagatccggaagcggcctctgatcgagacaaacggcgaaaccggggagatcgtgtgggataagggccgggattttgccaccgtgcggaaagtgctgagcatgccccaagtgaatatcgtgaaaaagaccgaggtgcagacaggcggcttcagcaaagagtctatcctgcccaagaggaacagcgataagctgatcgccagaaagaaggactgggaccctaagaagtacggcggcttcgacagccccaccgtggcctattctgtgctggtggtggccaaagtggaaaagggcaagtccaagaaactgaagagtgtgaaagagctgctggggatcaccatcatggaaagaagcagcttcgagaagaatcccatcgactttctggaagccaagggctacaaagaagtgaaaaaggacctgatcatcaagctgcctaagtactccctgttcgagctggaaaacggccggaagagaatgctggcctctgccggcgaactgcagaagggaaacgaactggccctgccctccaaatatgtgaacttcctgtacctggccagccactatgagaagctgaagggctcccccgaggataatgagcagaaacagctgtttgtggaacagcacaagcactacctggacgagatcatcgagcagatcagcgagttctccaagagagtgatcctggccgacgctaatctggacaaagtgctgtccgcctacaacaagcaccgggataagcccatcagagagcaggccgagaatatcatccacctgtttaccctgaccaatctgggagcccctgccgccttcaagtactttgacaccaccatcgaccggaagaggtacaccagcaccaaagaggtgctggacgccaccctgatccaccagagcatcaccggcctgtacgagacacggatcgacctgtctcagctgggaggcgacaaaaggccggcggccacgaaaaaggccggccaggcaaaaaagaaaaagggatcaggcaagcttgagggcagaggaagtcttctaacatgcggtgacgtggaggagaatcccggccctgctagcggtagcggcagcggtagcatgaccgagtacaagcccacggtgcgcctcgccacccgcgacgacgtcccccgggccgtacgcaccctcgccgccgcgttcgccgactaccccgccacgcgccacaccgtcgacccggaccgccacatcgagcgggtcaccgagctgcaagaactcttcctcacgcgcgtcgggctcgacatcggcaaggtgtgggtcgcggacgacggcgccgcggtggcggtctggaccacgccggagagcgtcgaagcgggggcggtgttcgccgagatcggcccgcgcatggccgagttgagcggttcccggctggccgcgcagcaacagatggaaggcctcctggcgccgcaccggcccaaggagcccgcgtggttcctggccaccgtcggcgtctcgcccgaccaccagggcaagggtctgggcagcgccgtcgtgctccccggagtggaggcggccgagcgcgccggggtgcccgccttcctggagacctccgcgccccgcaacctccccttctacgagcggctcggcttcaccgtcaccgccgacgtcgaggtgcccgaaggaccgcgcacctggtgcatgacccgcaagcccggtgcctgactcgagggatccagacatgataagatacattgatgagtttggacaaaccacaactagaatgcagtgaaaaaaatgctttatttgtgaaatttgtgatgctattgctttatttgtaaccattataagctgcaataaacaagttaacaacaacaattgcattcattttatgtttcaggttcagggggaggtgtgggaggttttttaaagcaagtaaaacctctacaaatgtggtatggctgattatgatcagtcgaccgatgcccttgagagccttcaacccagtcagctccttccggtgggcgcggggcatgactatcgtcgccgcacttatgactgtcttctttatcatgcaactcgtaggacaggtgccggcagcgctctgggtcattttcggcgaggaccgctttcgctggagcgcgacgatgatcggcctgtcgcttgcggtattcggaatcttgcacgccctcgctcaagccttcgtcactggtcccgccaccaaacgtttcggcgagaagcaggccattatcgccggcatggcggccgacgcgctgggctacgtcttgctggcgttcgcgacgcgaggctggatggccttccccattatgattcttctcgcttccggcggcatcgggatgcccgcgttgcaggccatgctgtccaggcaggtagatgacgaccatcagggacagcttcaaggatcgctcgcggctcttaccagccagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgtttttccataggctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccccctggaagctccctcgtgcgctctcctgttccgaccctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcaatgctcacgctgtaggtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccggtaactatcgtcttgagtccaacccggtaagacacgacttatcgccactggcagcagccactggtaacaggattagcagagcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacggctacactagaaggacagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttctacggggtctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaaggatcttcacctagatccttttaaattaaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtctgacagttaccaatgcttaatcagtgaggcacctatctcagcgatctgtctatttcgttcatccatagttgcctgactccccgtcgtgtagataactacgatacgggagggcttaccatctggccccagtgctgcaatgataccgcgagacccacgctcaccggctccagatttatcagcaataaaccagccagccggaagggccgagcgcagaagtggtcctgcaactttatccgcctccatccagtctattaattgttgccgggaagctagagtaagtagttcgccagttaatagtttgcgcaacgttgttgccattgctgcaggcatcgtggtgtcacgctcgtcgtttggtatggcttcattcagctccggttcccaacgatcaaggcgagttacatgatcccccatgttgtgcaaaaaagcggttagctccttcggtcctccgatcgttgtcagaagtaagttggccgcagtgttatcactcatggttatggcagcactgcataattctcttactgtcatgccatccgtaagatgcttttctgtgactggtgagtactcaaccaagtcattctgagaatagtgtatgcggcgaccgagttgctcttgcccggcgtcaacacgggataataccgcgccacatagcagaactttaaaagtgctcatcattggaaaacgttcttcggggcgaaaactctcaaggatcttaccgctgttgagatccagttcgatgtaacccactcgtgcacccaactgatcttcagcatcttttactttcaccagcgtttctgggtgagcaaaaacaggaaggcaaaatgccgcaaaaaagggaataagggcgacacggaaatgttgaatactcatactcttcctttttcaatattattgaagcatttatcagggttattgtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaataggggttccgcgcacatttccccgaaaagtgccacctgacgtctaagaaaccattattatcatgacattaacctataaaaataggcgtatcacgaggccctttcgtcttcaaggaattaattctatattctaaaaacacaaatgatacttctaaaaaaaatcatgaatggcatcaactctgaatcaaatctttgcagatgcacctacttctcatttccactgtcacatcatttttcc)表2在本发明提及的所有文献都在本申请中引用作为参考，就如同每一篇文献被单独引用作为参考那样。此外应理解，在阅读了本发明的上述讲授内容之后，本领域技术人员可以对本发明作各种改动或修改，这些等价形式同样落于本申请所附权利要求书所限定的范围。序列表<110>硅羿科技（上海）有限公司<120>一种可用于昆虫基因编辑的crispr/cas9载体<130>p2018-2396<160>25<170>patentinversion3.5<210>1<211>20<212>dna<213>人工序列(artificialsequence)<400>1gtcgccgccgggcgacgaga20<210>2<211>76<212>dna<213>人工序列(artificialsequence)<400>2gttttagagctagaaatagcaagttaaaataaggctagtccgttatcaacttgaaaaagt60ggcaccgagtcggtgc76<210>3<211>96<212>dna<213>人工序列(artificialsequence)<400>3gtcgccgccgggcgacgagagttttagagctagaaatagcaagttaaaataaggctagtc60cgttatcaacttgaaaaagtggcaccgagtcggtgc96<210>4<211>10663<212>dna<213>人工序列(artificialsequence)<400>4ggaaaaatgatgtgacagtggaaatgagaagtaggtgcatctgcaaagatttgattcaga60gttgatgccattcatgattttttttagaagtatcatttgtgtttttagaatatagaatta120attccttgaagacgaaagggcctcgtgatacgcctatttttataggttaatgtcatgata180ataatggtttcttagacgtcaggtggcacttttcggggaaatgtgcgcggaacccctatt240tgtttatttttctaaatacattcaaatatgtatccgctcatgagacaataaccctgataa300atgcttcaataatattgaaaaaggaagagtatgagtattcaacatttccgtgtcgccctt360attcccttttttgcggcattttgccttcctgtttttgctcacccagaaacgctggtgaaa420gtaaaagatgctgaagatcagttgggtgcacgagtgggttacatcgaactggatctcaac480agcggtaagatccttgagagttttcgccccgaagaacgttttccaatgatgagcactttt540aaagttctgctatgtggcgcggtattatcccgtgttgacgccgggcaagagcaactcggt600cgccgcatacactattctcagaatgacttggttgagtactcaccagtcacagaaaagcat660cttacggatggcatgacagtaagagaattatgcagtgctgccataaccatgagtgataac720actgcggccaacttacttctgacaacgatcggaggaccgaaggagctaaccgcttttttg780cacaacatgggggatcatgtaactcgccttgatcgttgggaaccggagctgaatgaagcc840ataccaaacgacgagcgtgacaccacgatgcctgcagcaatggcaacaacgttgcgcaaa900ctattaactggcgaactacttactctagcttcccggcaacaattaatagactggatggag960gcggataaagttgcaggaccacttctgcgctcggcccttccggctggctggtttattgct1020gataaatctggagccggtgagcgtgggtctcgcggtatcattgcagcactggggccagat1080ggtaagccctcccgtatcgtagttatctacacgacggggagtcaggcaactatggatgaa1140cgaaatagacagatcgctgagataggtgcctcactgattaagcattggtaactgtcagac1200caagtttactcatatatactttagattgatttaaaacttcatttttaatttaaaaggatc1260taggtgaagatcctttttgataatctcatgaccaaaatcccttaacgtgagttttcgttc1320cactgagcgtcagaccccgtagaaaagatcaaaggatcttcttgagatcctttttttctg1380cgcgtaatctgctgcttgcaaacaaaaaaaccaccgctaccagcggtggtttgtttgccg1440gatcaagagctaccaactctttttccgaaggtaactggcttcagcagagcgcagatacca1500aatactgtccttctagtgtagccgtagttaggccaccacttcaagaactctgtagcaccg1560cctacatacctcgctctgctaatcctgttaccagtggctgctgccagtggcgataagtcg1620tgtcttaccgggttggactcaagacgatagttaccggataaggcgcagcggtcgggctga1680acggggggttcgtgcacacagcccagcttggagcgaacgacctacaccgaactgagatac1740ctacagcgtgagcattgagaaagcgccacgcttcccgaagggagaaaggcggacaggtat1800ccggtaagcggcagggtcggaacaggagagcgcacgagggagcttccagggggaaacgcc1860tggtatctttatagtcctgtcgggtttcgccacctctgacttgagcgtcgatttttgtga1920tgctcgtcaggggggcggagcctatggaaaaacgccagcaacgcggcctttttacggttc1980ctggccttttgctggctggtaagagccgcgagcgatccttgaagctgtccctgatggtcg2040tcatctacctgcctggacagcatggcctgcaacgcgggcatcccgatgccgccggaagcg2100agaagaatcataatggggaaggccatccagcctcgcgtcgcgaacgccagcaagacgtag2160cccagcgcgtcggccgccatgccggcgataatggcctgcttctcgccgaaacgtttggtg2220gcgggaccagtgacgaaggcttgagcgagggcgtgcaagattccgaataccgcaagcgac2280aggccgatcatcgtcgcgctccagcgaaagcggtcctcgccgaaaatgacccagagcgct2340gccggcacctgtcctacgagttgcatgataaagaagacagtcataagtgcggcgacgata2400gtcatgccccgcgcccaccggaaggagctgactgggttgaaggctctcaagggcatcggt2460cgactgatcataatcagccataccacatttgtagaggttttacttgctttaaaaaacctc2520ccacacctccccctgaacctgaaacataaaatgaatgcaattgttgttgttaacttgttt2580attgcagcttataatggttacaaataaagcaatagcatcacaaatttcacaaataaagca2640tttttttcactgcattctagttgtggtttgtccaaactcatcaatgtatcttatcatgtc2700tggatccctcgagtcaggcaccgggcttgcgggtcatgcaccaggtgcgcggtccttcgg2760gcacctcgacgtcggcggtgacggtgaagccgagccgctcgtagaaggggaggttgcggg2820gcgcggaggtctccaggaaggcgggcaccccggcgcgctcggccgcctccactccgggga2880gcacgacggcgctgcccagacccttgccctggtggtcgggcgagacgccgacggtggcca2940ggaaccacgcgggctccttgggccggtgcggcgccaggaggccttccatctgttgctgcg3000cggccagccgggaaccgctcaactcggccatgcgcgggccgatctcggcgaacaccgccc3060ccgcttcgacgctctccggcgtggtccagaccgccaccgcggcgccgtcgtccgcgaccc3120acaccttgccgatgtcgagcccgacgcgcgtgaggaagagttcttgcagctcggtgaccc3180gctcgatgtggcggtccgggtcgacggtgtggcgcgtggcggggtagtcggcgaacgcgg3240cggcgagggtgcgtacggcccgggggacgtcgtcgcgggtggcgaggcgcaccgtgggct3300tgtactcggtcatgctaccgctgccgctaccgctagcagggccgggattctcctccacgt3360caccgcatgttagaagacttcctctgccctcaagcttgcctgatccctttttcttttttg3420cctggccggcctttttcgtggccgccggccttttgtcgcctcccagctgagacaggtcga3480tccgtgtctcgtacaggccggtgatgctctggtggatcagggtggcgtccagcacctctt3540tggtgctggtgtacctcttccggtcgatggtggtgtcaaagtacttgaaggcggcagggg3600ctcccagattggtcagggtaaacaggtggatgatattctcggcctgctctctgatgggct3660tatcccggtgcttgttgtaggcggacagcactttgtccagattagcgtcggccaggatca3720ctctcttggagaactcgctgatctgctcgatgatctcgtccaggtagtgcttgtgctgtt3780ccacaaacagctgtttctgctcattatcctcgggggagcccttcagcttctcatagtggc3840tggccaggtacaggaagttcacatatttggagggcagggccagttcgtttcccttctgca3900gttcgccggcagaggccagcattctcttccggccgttttccagctcgaacagggagtact3960taggcagcttgatgatcaggtcctttttcacttctttgtagcccttggcttccagaaagt4020cgatgggattcttctcgaagctgcttctttccatgatggtgatccccagcagctctttca4080cactcttcagtttcttggacttgcccttttccactttggccaccaccagcacagaatagg4140ccacggtggggctgtcgaagccgccgtacttcttagggtcccagtccttctttctggcga4200tcagcttatcgctgttcctcttgggcaggatagactctttgctgaagccgcctgtctgca4260cctcggtctttttcacgatattcacttggggcatgctcagcactttccgcacggtggcaa4320aatcccggcccttatcccacacgatctccccggtttcgccgtttgtctcgatcagaggcc4380gcttccggatctcgccgttggccagggtaatctcggtcttgaaaaagttcatgatgttgc4440tgtagaagaagtacttggcggtagccttgccgatttcctgctcgctcttggcgatcatct4500tccgcacgtcgtacaccttgtagtcgccgtacacgaactcgctttccagcttagggtact4560ttttgatcagggcggttcccacgacggcgttcaggtaggcgtcgtgggcgtggtggtagt4620tgttgatctcgcgcactttgtaaaactggaaatccttccggaaatcggacaccagcttgg4680acttcagggtgatcactttcacttcccggatcagcttgtcattctcgtcgtacttagtgt4740tcatccgggagtccaggatctgtgccacgtgctttgtgatctgccgggtttccaccagct4800gtctcttgatgaagccggccttatccagttcgctcaggccgcctctctcggccttggtca4860gattgtcgaactttctctgggtaatcagcttggcgttcagcagctgccgccagtagttct4920tcatcttcttcacgacctcttcggagggcacgttgtcgctcttgccccggttcttgtcgc4980ttctggtcagcaccttgttgtcgatggagtcgtccttcagaaagctctgaggcacgatat5040ggtccacatcgtagtcggacagccggttgatgtccagttcctggtccacgtacatatccc5100gcccattctgcaggtagtacaggtacagcttctcgttctgcagctgggtgttttccacgg5160ggtgttctttcaggatctggctgcccagctctttgatgccctcttcgatccgcttcattc5220tctcgcggctgttcttctgtcccttctgggtggtctggttctctctggccatttcgatca5280cgatgttctcgggcttgtgccggcccatcactttcacgagctcgtccaccaccttcactg5340tctgcaggatgcccttcttaatggcggggctgccggccagattggcaatgtgctcgtgca5400ggctatcgccctggccggacacctgggctttctggatgtcctctttaaaggtcaggctgt5460cgtcgtggatcagctgcatgaagtttctgttggcgaagccgtcggacttcaggaaatcca5520ggattgtcttgccggactgcttgtcccggatgccgttgatcagcttccggctcagcctgc5580cccagccggtgtatctccgccgcttcagctgcttcatcactttgtcgtcgaacaggtggg5640cataggttttcagccgttcctcgatcatctctctgtcctcaaacagtgtcagggtcagca5700cgatatcttccagaatgtcctcgttttcctcattgtccaggaagtccttgtccttgataa5760ttttcagcagatcgtggtatgtgcccagggaggcgttgaaccgatcttccacgccggaga5820tttccacggagtcgaagcactcgattttcttgaagtagtcctctttcagctgcttcacgg5880tcactttccggttggtcttgaacagcaggtccacgatggcctttttctgctcgccgctca5940ggaaggcgggctttctcattccctcggtcacgtatttcactttggtcagctcgttataca6000cggtgaagtactcgtacagcaggctgtgcttgggcagcaccttctcgttgggcaggttct6060tatcgaagttggtcatccgctcgatgaagctctgggcggaagcgcccttgtccaccactt6120cctcgaagttccagggggtgatggtttcctcgctctttctggtcatccaggcgaatctgc6180tgtttcccctggccagagggcccacgtagtaggggatgcggaaggtcaggatcttctcga6240tcttttcccggttgtccttcaggaatgggtaaaaatcttcctgccgccgcagaatggcgt6300gcagctctcccaggtggatctggtgggggatgctgccgttgtcgaaggtccgctgcttcc6360gcagcaggtcctctctgttcagcttcacgagcagttcctcggtgccgtccatcttttcca6420ggatgggcttgatgaacttgtagaactcttcctggctggctccgccgtcaatgtagccgg6480cgtagccgttcttgctctggtcgaagaaaatctctttgtacttctcaggcagctgctgcc6540gcacgagagctttcagcagggtcaggtcctggtggtgctcgtcgtatctcttgatcatag6600aggcgctcaggggggccttggtgatctcggtgttcactctcaggatgtcgctcagcagga6660tggcgtcggacaggttcttggcggccagaaacaggtcggcgtactggtcgccgatctggg6720ccagcaggttgtccaggtcgtcgtcgtaggtgtccttgctcagctgcagtttggcatcct6780cggccaggtcgaagttgctcttgaagttgggggtcaggcccaggctcagggcaatcaggt6840ttccgaacaggccattcttcttctcgccgggcagctgggcgatcagattttccagccgtc6900tgctcttgctcagtctggcagacaggatggccttggcgtccacgccgctggcgttgatgg6960ggttttcctcgaacagctggttgtaggtctgcaccagctggatgaacagcttgtccacgt7020cgctgttgtcggggttcaggtcgccctcgatcaggaagtggccccggaacttgatcatgt7080gggccagggccagatagatcagccgcaggtcggccttgtcggtgctgtccaccagtttct7140ttctcaggtggtagatggtggggtacttctcgtggtaggccacctcgtccacgatgttgc7200cgaagatggggtgccgctcgtgcttcttatcctcttccaccaggaaggactcttccagtc7260tgtggaagaagctgtcgtccaccttggccatctcgttgctgaagatctcttgcagatagc7320agatccggttcttccgtctggtgtatcttcttctggcggttctcttcagccgggtggcct7380cggctgtttcgccgctgtcgaacagcagggctccgatcaggttcttcttgatgctgtgcc7440ggtcggtgttgcccagcaccttgaatttcttgctgggcaccttgtactcgtcggtgatca7500cggcccagcccacagagttggtgccgatgtccaggccgatgctgtacttcttgtcggctg7560ctgggactccgtggataccgaccttccgcttcttctttggggccatcttatcgtcatcgt7620ctttgtaatcaatatcatgatccttgtagtctccgtcgtggtccttatagtccatgaatt7680ccaccacactggactagtaggtaccccgatccggggtctctggattagacgactgctggc7740tgatggagcggctttgtgtcgggaggagtatccacacagcacaagaactcaaacggtagt7800gatatgaaaactggtcccgcagcccgttttataaagcccgcttagcgctaaaaccttatc7860agccgcaatgctctccgctctcaagtcgcgttcaaaacttttaccatatatgggtatgaa7920ttgttcgccgattgggttttcatttacggagaatttcctccgcaactgggtgttcgtgct7980tgtgtgtgtcttgtatatcgccatgaagagtgtacttccatgggtcctatcagttgcggc8040atgtgattcaatttgggggttactcatattcgatacttcagctggttatctttatgctgt8100gatggtcacggtgcttttacacattttttccccaactactcattgtatgccaaaacatac8160atatgtacatgtgtttatagtgggtttattagtaatgtccagcgatattaaaacattaca8220gttaaataatattcatattagcttacgtttactgctatctgcacttcaaattggttattc8280agggacatttgtgcaacacacatcttctagtaggggcatcactagtcgtagaaaacacac8340ctaacttaagccggttttaacaataacggcgaaacagctgccagacgagagaaaagtaca8400cagcaagaagcatttctataatgatgccgtcctaaatatgcagcatttttgctcaaattg8460atcatttcgttgcatcgctcaggtggtatagtatttggctattgaatgtgattgctatct8520taatagttttgaatagtaatagtgcaaatattcattcataaaaaataggtaatcgatttt8580ctcagtgcagtctatgcgtttgcgcaaacaggagacgaagagagagacgcctcacacaca8640agcacatgcggtctaagctgctctccctctttttctctctctctctgcttggcggcagtt8700cttccagaggctgtgacgtcaggtaagctatttcgtctcggtgtgctgttagcccgtaac8760gataagctgtccgattcctaattctctttcaaataatttgttttatttttttttttgtgt8820gttttgaagtcaaacagcttcataaacaatattaaatatcgcaattgttattgcaaatgc8880attcgtatcaggcgccttgatgtttttcggccaaaacttcgtctgctctttgtcagcgta8940attgcgtttgccggtcgtgccgtcttgctcgcacgcgcttgcaaacaaaaagtgaaagag9000ggaaagccactgtgttgttgcttttgttgctatcgttggctgcacacacatggcaataag9060taaccatattgtagacttatttattttagcgttttcgttgactaatctttgtttaaataa9120ttgcctatttcgagccaaaatgacgcataaaggacacggcaacatgcggtgcgttgagca9180tgcgtacgagatggcgctagagagagcgagataaaaagaaaaaaacaaccggcgagtggc9240gagacacgaataatacacacacgcacacatggtcaaaggagtggctccaacgtcggtgac9300gtcgcgcccagccacagaccccgccacgccccgccccacccttccttccgctgccccgca9360cccctttccttccactaccgttttgccgcatttggtattgccatctcgcttttttggctt9420tactttgctttttgaataagccaagaaactgaaatcaaagcggacagtccgaaaattgaa9480actattccaaaaaacaacaaccgcacatgcactgcgttcggcaaaagacgcagaaatcag9540cctgtggtctggcatcatcttaaaatatcatcagtatcatcattgccattataaatacat9600aagaaaatgagaatacattaaaggtatatacatacaatctttgtcatagtcaacatattt9660tgcaacaaaattatctatagataacatgtttttgtagaaaattccactaactgattttcg9720tataaatacaaattattctcattcttcaaaatactcgccgacgtcatcgcgaaattcctc9780agcattcttcgacaaatgaagagagacagcgagtgacacaatcttatcttgccgacgagt9840ctttgaaaatggccccgggtaacggtgaaaaacatatagccaagaatggagcgatcgcca9900ggcgatggaaattggcttcaaattgctccgaatatggccagttggtcagttggcattgga9960ttgtggagagatactagatcagctcgttctccttctcctccgcattctcctactccgccc10020gaagacgcttctttgatcgcttctttctcgaatgtgtacatattcattatgtatttggtg10080tttggcgacgatcagccagctttaatggccataaaacgtgtcttggtttgtgggccacat10140aacaggcagcgagatctgttcgacttgcagcctgaaatacggcacgagtaggaaaagccg10200agtcaaatgccgaatgcagagtctcattacagcacaatcaactcaagaaaaactcgacac10260ttttttaccatttgcacttaaatccttttttattcgttatgtatactttttttggtccct10320aaccaaaacaaaaccaaactctcttagtcgtgcctctatatttaaaactatcaatttatt10380atagtcaataaatcgaactgtgttttcaacaaacgaacaataggacactttgattctaaa10440ggaaattttgaaaatcttaagcagagggttcttaagaccatttgccaattcttataattc10500tcaactgctctttcctgatgttgatcatttatataggtatgttttcctcaatacttcgga10560agagcgagctcttccgttttagagctagaaatagcaagttaaaataaggctagtccgtta10620tcaacttgaaaaagtggcaccgagtcggtgcttttttagatct10663<210>5<211>24<212>dna<213>人工序列(artificialsequence)<400>5ttcggtcgccgccgggcgacgaga24<210>6<211>24<212>dna<213>人工序列(artificialsequence)<400>6aactctcgtcgcccggcggcgacc24<210>7<211>24<212>dna<213>人工序列(artificialsequence)<400>7ttcggacttgcgactacaacccga24<210>8<211>24<212>dna<213>人工序列(artificialsequence)<400>8aactcgggttgtagtcgcaagtcc24<210>9<211>10666<212>dna<213>人工序列(artificialsequence)<400>9agatctaaaaaagcaccgactcggtgccactttttcaagttgataacggactagccttat60tttaacttgctatttctagctctaaaactctcgtcgcccggcggcgaccgaagtattgag120gaaaacatacctatataaatgatcaacatcaggaaagagcagttgagaattataagaatt180ggcaaatggtcttaagaaccctctgcttaagattttcaaaatttcctttagaatcaaagt240gtcctattgttcgtttgttgaaaacacagttcgatttattgactataataaattgatagt300tttaaatatagaggcacgactaagagagtttggttttgttttggttagggaccaaaaaaa360gtatacataacgaataaaaaaggatttaagtgcaaatggtaaaaaagtgtcgagtttttc420ttgagttgattgtgctgtaatgagactctgcattcggcatttgactcggcttttcctact480cgtgccgtatttcaggctgcaagtcgaacagatctcgctgcctgttatgtggcccacaaa540ccaagacacgttttatggccattaaagctggctgatcgtcgccaaacaccaaatacataa600tgaatatgtacacattcgagaaagaagcgatcaaagaagcgtcttcgggcggagtaggag660aatgcggaggagaaggagaacgagctgatctagtatctctccacaatccaatgccaactg720accaactggccatattcggagcaatttgaagccaatttccatcgcctggcgatcgctcca780ttcttggctatatgtttttcaccgttacccggggccattttcaaagactcgtcggcaaga840taagattgtgtcactcgctgtctctcttcatttgtcgaagaatgctgaggaatttcgcga900tgacgtcggcgagtattttgaagaatgagaataatttgtatttatacgaaaatcagttag960tggaattttctacaaaaacatgttatctatagataattttgttgcaaaatatgttgacta1020tgacaaagattgtatgtatatacctttaatgtattctcattttcttatgtatttataatg1080gcaatgatgatactgatgatattttaagatgatgccagaccacaggctgatttctgcgtc1140ttttgccgaacgcagtgcatgtgcggttgttgttttttggaatagtttcaattttcggac1200tgtccgctttgatttcagtttcttggcttattcaaaaagcaaagtaaagccaaaaaagcg1260agatggcaataccaaatgcggcaaaacggtagtggaaggaaaggggtgcggggcagcgga1320aggaagggtggggcggggcgtggcggggtctgtggctgggcgcgacgtcaccgacgttgg1380agccactcctttgaccatgtgtgcgtgtgtgtattattcgtgtctcgccactcgccggtt1440gtttttttctttttatctcgctctctctagcgccatctcgtacgcatgctcaacgcaccg1500catgttgccgtgtcctttatgcgtcattttggctcgaaataggcaattatttaaacaaag1560attagtcaacgaaaacgctaaaataaataagtctacaatatggttacttattgccatgtg1620tgtgcagccaacgatagcaacaaaagcaacaacacagtggctttccctctttcacttttt1680gtttgcaagcgcgtgcgagcaagacggcacgaccggcaaacgcaattacgctgacaaaga1740gcagacgaagttttggccgaaaaacatcaaggcgcctgatacgaatgcatttgcaataac1800aattgcgatatttaatattgtttatgaagctgtttgacttcaaaacacacaaaaaaaaaa1860ataaaacaaattatttgaaagagaattaggaatcggacagcttatcgttacgggctaaca1920gcacaccgagacgaaatagcttacctgacgtcacagcctctggaagaactgccgccaagc1980agagagagagagaaaaagagggagagcagcttagaccgcatgtgcttgtgtgtgaggcgt2040ctctctcttcgtctcctgtttgcgcaaacgcatagactgcactgagaaaatcgattacct2100attttttatgaatgaatatttgcactattactattcaaaactattaagatagcaatcaca2160ttcaatagccaaatactataccacctgagcgatgcaacgaaatgatcaatttgagcaaaa2220atgctgcatatttaggacggcatcattatagaaatgcttcttgctgtgtacttttctctc2280gtctggcagctgtttcgccgttattgttaaaaccggcttaagttaggtgtgttttctacg2340actagtgatgcccctactagaagatgtgtgttgcacaaatgtccctgaataaccaatttg2400aagtgcagatagcagtaaacgtaagctaatatgaatattatttaactgtaatgttttaat2460atcgctggacattactaataaacccactataaacacatgtacatatgtatgttttggcat2520acaatgagtagttggggaaaaaatgtgtaaaagcaccgtgaccatcacagcataaagata2580accagctgaagtatcgaatatgagtaacccccaaattgaatcacatgccgcaactgatag2640gacccatggaagtacactcttcatggcgatatacaagacacacacaagcacgaacaccca2700gttgcggaggaaattctccgtaaatgaaaacccaatcggcgaacaattcatacccatata2760tggtaaaagttttgaacgcgacttgagagcggagagcattgcggctgataaggttttagc2820gctaagcgggctttataaaacgggctgcgggaccagttttcatatcactaccgtttgagt2880tcttgtgctgtgtggatactcctcccgacacaaagccgctccatcagccagcagtcgtct2940aatccagagaccccggatcggggtacctactagtccagtgtggtggaattcatggactat3000aaggaccacgacggagactacaaggatcatgatattgattacaaagacgatgacgataag3060atggccccaaagaagaagcggaaggtcggtatccacggagtcccagcagccgacaagaag3120tacagcatcggcctggacatcggcaccaactctgtgggctgggccgtgatcaccgacgag3180tacaaggtgcccagcaagaaattcaaggtgctgggcaacaccgaccggcacagcatcaag3240aagaacctgatcggagccctgctgttcgacagcggcgaaacagccgaggccacccggctg3300aagagaaccgccagaagaagatacaccagacggaagaaccggatctgctatctgcaagag3360atcttcagcaacgagatggccaaggtggacgacagcttcttccacagactggaagagtcc3420ttcctggtggaagaggataagaagcacgagcggcaccccatcttcggcaacatcgtggac3480gaggtggcctaccacgagaagtaccccaccatctaccacctgagaaagaaactggtggac3540agcaccgacaaggccgacctgcggctgatctatctggccctggcccacatgatcaagttc3600cggggccacttcctgatcgagggcgacctgaaccccgacaacagcgacgtggacaagctg3660ttcatccagctggtgcagacctacaaccagctgttcgaggaaaaccccatcaacgccagc3720ggcgtggacgccaaggccatcctgtctgccagactgagcaagagcagacggctggaaaat3780ctgatcgcccagctgcccggcgagaagaagaatggcctgttcggaaacctgattgccctg3840agcctgggcctgacccccaacttcaagagcaacttcgacctggccgaggatgccaaactg3900cagctgagcaaggacacctacgacgacgacctggacaacctgctggcccagatcggcgac3960cagtacgccgacctgtttctggccgccaagaacctgtccgacgccatcctgctgagcgac4020atcctgagagtgaacaccgagatcaccaaggcccccctgagcgcctctatgatcaagaga4080tacgacgagcaccaccaggacctgaccctgctgaaagctctcgtgcggcagcagctgcct4140gagaagtacaaagagattttcttcgaccagagcaagaacggctacgccggctacattgac4200ggcggagccagccaggaagagttctacaagttcatcaagcccatcctggaaaagatggac4260ggcaccgaggaactgctcgtgaagctgaacagagaggacctgctgcggaagcagcggacc4320ttcgacaacggcagcatcccccaccagatccacctgggagagctgcacgccattctgcgg4380cggcaggaagatttttacccattcctgaaggacaaccgggaaaagatcgagaagatcctg4440accttccgcatcccctactacgtgggccctctggccaggggaaacagcagattcgcctgg4500atgaccagaaagagcgaggaaaccatcaccccctggaacttcgaggaagtggtggacaag4560ggcgcttccgcccagagcttcatcgagcggatgaccaacttcgataagaacctgcccaac4620gagaaggtgctgcccaagcacagcctgctgtacgagtacttcaccgtgtataacgagctg4680accaaagtgaaatacgtgaccgagggaatgagaaagcccgccttcctgagcggcgagcag4740aaaaaggccatcgtggacctgctgttcaagaccaaccggaaagtgaccgtgaagcagctg4800aaagaggactacttcaagaaaatcgagtgcttcgactccgtggaaatctccggcgtggaa4860gatcggttcaacgcctccctgggcacataccacgatctgctgaaaattatcaaggacaag4920gacttcctggacaatgaggaaaacgaggacattctggaagatatcgtgctgaccctgaca4980ctgtttgaggacagagagatgatcgaggaacggctgaaaacctatgcccacctgttcgac5040gacaaagtgatgaagcagctgaagcggcggagatacaccggctggggcaggctgagccgg5100aagctgatcaacggcatccgggacaagcagtccggcaagacaatcctggatttcctgaag5160tccgacggcttcgccaacagaaacttcatgcagctgatccacgacgacagcctgaccttt5220aaagaggacatccagaaagcccaggtgtccggccagggcgatagcctgcacgagcacatt5280gccaatctggccggcagccccgccattaagaagggcatcctgcagacagtgaaggtggtg5340gacgagctcgtgaaagtgatgggccggcacaagcccgagaacatcgtgatcgaaatggcc5400agagagaaccagaccacccagaagggacagaagaacagccgcgagagaatgaagcggatc5460gaagagggcatcaaagagctgggcagccagatcctgaaagaacaccccgtggaaaacacc5520cagctgcagaacgagaagctgtacctgtactacctgcagaatgggcgggatatgtacgtg5580gaccaggaactggacatcaaccggctgtccgactacgatgtggaccatatcgtgcctcag5640agctttctgaaggacgactccatcgacaacaaggtgctgaccagaagcgacaagaaccgg5700ggcaagagcgacaacgtgccctccgaagaggtcgtgaagaagatgaagaactactggcgg5760cagctgctgaacgccaagctgattacccagagaaagttcgacaatctgaccaaggccgag5820agaggcggcctgagcgaactggataaggccggcttcatcaagagacagctggtggaaacc5880cggcagatcacaaagcacgtggcacagatcctggactcccggatgaacactaagtacgac5940gagaatgacaagctgatccgggaagtgaaagtgatcaccctgaagtccaagctggtgtcc6000gatttccggaaggatttccagttttacaaagtgcgcgagatcaacaactaccaccacgcc6060cacgacgcctacctgaacgccgtcgtgggaaccgccctgatcaaaaagtaccctaagctg6120gaaagcgagttcgtgtacggcgactacaaggtgtacgacgtgcggaagatgatcgccaag6180agcgagcaggaaatcggcaaggctaccgccaagtacttcttctacagcaacatcatgaac6240tttttcaagaccgagattaccctggccaacggcgagatccggaagcggcctctgatcgag6300acaaacggcgaaaccggggagatcgtgtgggataagggccgggattttgccaccgtgcgg6360aaagtgctgagcatgccccaagtgaatatcgtgaaaaagaccgaggtgcagacaggcggc6420ttcagcaaagagtctatcctgcccaagaggaacagcgataagctgatcgccagaaagaag6480gactgggaccctaagaagtacggcggcttcgacagccccaccgtggcctattctgtgctg6540gtggtggccaaagtggaaaagggcaagtccaagaaactgaagagtgtgaaagagctgctg6600gggatcaccatcatggaaagaagcagcttcgagaagaatcccatcgactttctggaagcc6660aagggctacaaagaagtgaaaaaggacctgatcatcaagctgcctaagtactccctgttc6720gagctggaaaacggccggaagagaatgctggcctctgccggcgaactgcagaagggaaac6780gaactggccctgccctccaaatatgtgaacttcctgtacctggccagccactatgagaag6840ctgaagggctcccccgaggataatgagcagaaacagctgtttgtggaacagcacaagcac6900tacctggacgagatcatcgagcagatcagcgagttctccaagagagtgatcctggccgac6960gctaatctggacaaagtgctgtccgcctacaacaagcaccgggataagcccatcagagag7020caggccgagaatatcatccacctgtttaccctgaccaatctgggagcccctgccgccttc7080aagtactttgacaccaccatcgaccggaagaggtacaccagcaccaaagaggtgctggac7140gccaccctgatccaccagagcatcaccggcctgtacgagacacggatcgacctgtctcag7200ctgggaggcgacaaaaggccggcggccacgaaaaaggccggccaggcaaaaaagaaaaag7260ggatcaggcaagcttgagggcagaggaagtcttctaacatgcggtgacgtggaggagaat7320cccggccctgctagcggtagcggcagcggtagcatgaccgagtacaagcccacggtgcgc7380ctcgccacccgcgacgacgtcccccgggccgtacgcaccctcgccgccgcgttcgccgac7440taccccgccacgcgccacaccgtcgacccggaccgccacatcgagcgggtcaccgagctg7500caagaactcttcctcacgcgcgtcgggctcgacatcggcaaggtgtgggtcgcggacgac7560ggcgccgcggtggcggtctggaccacgccggagagcgtcgaagcgggggcggtgttcgcc7620gagatcggcccgcgcatggccgagttgagcggttcccggctggccgcgcagcaacagatg7680gaaggcctcctggcgccgcaccggcccaaggagcccgcgtggttcctggccaccgtcggc7740gtctcgcccgaccaccagggcaagggtctgggcagcgccgtcgtgctccccggagtggag7800gcggccgagcgcgccggggtgcccgccttcctggagacctccgcgccccgcaacctcccc7860ttctacgagcggctcggcttcaccgtcaccgccgacgtcgaggtgcccgaaggaccgcgc7920acctggtgcatgacccgcaagcccggtgcctgactcgagggatccagacatgataagata7980cattgatgagtttggacaaaccacaactagaatgcagtgaaaaaaatgctttatttgtga8040aatttgtgatgctattgctttatttgtaaccattataagctgcaataaacaagttaacaa8100caacaattgcattcattttatgtttcaggttcagggggaggtgtgggaggttttttaaag8160caagtaaaacctctacaaatgtggtatggctgattatgatcagtcgaccgatgcccttga8220gagccttcaacccagtcagctccttccggtgggcgcggggcatgactatcgtcgccgcac8280ttatgactgtcttctttatcatgcaactcgtaggacaggtgccggcagcgctctgggtca8340ttttcggcgaggaccgctttcgctggagcgcgacgatgatcggcctgtcgcttgcggtat8400tcggaatcttgcacgccctcgctcaagccttcgtcactggtcccgccaccaaacgtttcg8460gcgagaagcaggccattatcgccggcatggcggccgacgcgctgggctacgtcttgctgg8520cgttcgcgacgcgaggctggatggccttccccattatgattcttctcgcttccggcggca8580tcgggatgcccgcgttgcaggccatgctgtccaggcaggtagatgacgaccatcagggac8640agcttcaaggatcgctcgcggctcttaccagccagcaaaaggccaggaaccgtaaaaagg8700ccgcgttgctggcgtttttccataggctccgcccccctgacgagcatcacaaaaatcgac8760gctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccccctg8820gaagctccctcgtgcgctctcctgttccgaccctgccgcttaccggatacctgtccgcct8880ttctcccttcgggaagcgtggcgctttctcaatgctcacgctgtaggtatctcagttcgg8940tgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgct9000gcgccttatccggtaactatcgtcttgagtccaacccggtaagacacgacttatcgccac9060tggcagcagccactggtaacaggattagcagagcgaggtatgtaggcggtgctacagagt9120tcttgaagtggtggcctaactacggctacactagaaggacagtatttggtatctgcgctc9180tgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaacca9240ccgctggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggat9300ctcaagaagatcctttgatcttttctacggggtctgacgctcagtggaacgaaaactcac9360gttaagggattttggtcatgagattatcaaaaaggatcttcacctagatccttttaaatt9420aaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtctgacagttacc9480aatgcttaatcagtgaggcacctatctcagcgatctgtctatttcgttcatccatagttg9540cctgactccccgtcgtgtagataactacgatacgggagggcttaccatctggccccagtg9600ctgcaatgataccgcgagacccacgctcaccggctccagatttatcagcaataaaccagc9660cagccggaagggccgagcgcagaagtggtcctgcaactttatccgcctccatccagtcta9720ttaattgttgccgggaagctagagtaagtagttcgccagttaatagtttgcgcaacgttg9780ttgccattgctgcaggcatcgtggtgtcacgctcgtcgtttggtatggcttcattcagct9840ccggttcccaacgatcaaggcgagttacatgatcccccatgttgtgcaaaaaagcggtta9900gctccttcggtcctccgatcgttgtcagaagtaagttggccgcagtgttatcactcatgg9960ttatggcagcactgcataattctcttactgtcatgccatccgtaagatgcttttctgtga10020ctggtgagtactcaaccaagtcattctgagaatagtgtatgcggcgaccgagttgctctt10080gcccggcgtcaacacgggataataccgcgccacatagcagaactttaaaagtgctcatca10140ttggaaaacgttcttcggggcgaaaactctcaaggatcttaccgctgttgagatccagtt10200cgatgtaacccactcgtgcacccaactgatcttcagcatcttttactttcaccagcgttt10260ctgggtgagcaaaaacaggaaggcaaaatgccgcaaaaaagggaataagggcgacacgga10320aatgttgaatactcatactcttcctttttcaatattattgaagcatttatcagggttatt10380gtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaataggggttccgc10440gcacatttccccgaaaagtgccacctgacgtctaagaaaccattattatcatgacattaa10500cctataaaaataggcgtatcacgaggccctttcgtcttcaaggaattaattctatattct10560aaaaacacaaatgatacttctaaaaaaaatcatgaatggcatcaactctgaatcaaatct10620ttgcagatgcacctacttctcatttccactgtcacatcatttttcc10666<210>10<211>10666<212>dna<213>人工序列(artificialsequence)<400>10agatctaaaaaagcaccgactcggtgccactttttcaagttgataacggactagccttat60tttaacttgctatttctagctctaaaactcgggttgtagtcgcaagtccgaagtattgag120gaaaacatacctatataaatgatcaacatcaggaaagagcagttgagaattataagaatt180ggcaaatggtcttaagaaccctctgcttaagattttcaaaatttcctttagaatcaaagt240gtcctattgttcgtttgttgaaaacacagttcgatttattgactataataaattgatagt300tttaaatatagaggcacgactaagagagtttggttttgttttggttagggaccaaaaaaa360gtatacataacgaataaaaaaggatttaagtgcaaatggtaaaaaagtgtcgagtttttc420ttgagttgattgtgctgtaatgagactctgcattcggcatttgactcggcttttcctact480cgtgccgtatttcaggctgcaagtcgaacagatctcgctgcctgttatgtggcccacaaa540ccaagacacgttttatggccattaaagctggctgatcgtcgccaaacaccaaatacataa600tgaatatgtacacattcgagaaagaagcgatcaaagaagcgtcttcgggcggagtaggag660aatgcggaggagaaggagaacgagctgatctagtatctctccacaatccaatgccaactg720accaactggccatattcggagcaatttgaagccaatttccatcgcctggcgatcgctcca780ttcttggctatatgtttttcaccgttacccggggccattttcaaagactcgtcggcaaga840taagattgtgtcactcgctgtctctcttcatttgtcgaagaatgctgaggaatttcgcga900tgacgtcggcgagtattttgaagaatgagaataatttgtatttatacgaaaatcagttag960tggaattttctacaaaaacatgttatctatagataattttgttgcaaaatatgttgacta1020tgacaaagattgtatgtatatacctttaatgtattctcattttcttatgtatttataatg1080gcaatgatgatactgatgatattttaagatgatgccagaccacaggctgatttctgcgtc1140ttttgccgaacgcagtgcatgtgcggttgttgttttttggaatagtttcaattttcggac1200tgtccgctttgatttcagtttcttggcttattcaaaaagcaaagtaaagccaaaaaagcg1260agatggcaataccaaatgcggcaaaacggtagtggaaggaaaggggtgcggggcagcgga1320aggaagggtggggcggggcgtggcggggtctgtggctgggcgcgacgtcaccgacgttgg1380agccactcctttgaccatgtgtgcgtgtgtgtattattcgtgtctcgccactcgccggtt1440gtttttttctttttatctcgctctctctagcgccatctcgtacgcatgctcaacgcaccg1500catgttgccgtgtcctttatgcgtcattttggctcgaaataggcaattatttaaacaaag1560attagtcaacgaaaacgctaaaataaataagtctacaatatggttacttattgccatgtg1620tgtgcagccaacgatagcaacaaaagcaacaacacagtggctttccctctttcacttttt1680gtttgcaagcgcgtgcgagcaagacggcacgaccggcaaacgcaattacgctgacaaaga1740gcagacgaagttttggccgaaaaacatcaaggcgcctgatacgaatgcatttgcaataac1800aattgcgatatttaatattgtttatgaagctgtttgacttcaaaacacacaaaaaaaaaa1860ataaaacaaattatttgaaagagaattaggaatcggacagcttatcgttacgggctaaca1920gcacaccgagacgaaatagcttacctgacgtcacagcctctggaagaactgccgccaagc1980agagagagagagaaaaagagggagagcagcttagaccgcatgtgcttgtgtgtgaggcgt2040ctctctcttcgtctcctgtttgcgcaaacgcatagactgcactgagaaaatcgattacct2100attttttatgaatgaatatttgcactattactattcaaaactattaagatagcaatcaca2160ttcaatagccaaatactataccacctgagcgatgcaacgaaatgatcaatttgagcaaaa2220atgctgcatatttaggacggcatcattatagaaatgcttcttgctgtgtacttttctctc2280gtctggcagctgtttcgccgttattgttaaaaccggcttaagttaggtgtgttttctacg2340actagtgatgcccctactagaagatgtgtgttgcacaaatgtccctgaataaccaatttg2400aagtgcagatagcagtaaacgtaagctaatatgaatattatttaactgtaatgttttaat2460atcgctggacattactaataaacccactataaacacatgtacatatgtatgttttggcat2520acaatgagtagttggggaaaaaatgtgtaaaagcaccgtgaccatcacagcataaagata2580accagctgaagtatcgaatatgagtaacccccaaattgaatcacatgccgcaactgatag2640gacccatggaagtacactcttcatggcgatatacaagacacacacaagcacgaacaccca2700gttgcggaggaaattctccgtaaatgaaaacccaatcggcgaacaattcatacccatata2760tggtaaaagttttgaacgcgacttgagagcggagagcattgcggctgataaggttttagc2820gctaagcgggctttataaaacgggctgcgggaccagttttcatatcactaccgtttgagt2880tcttgtgctgtgtggatactcctcccgacacaaagccgctccatcagccagcagtcgtct2940aatccagagaccccggatcggggtacctactagtccagtgtggtggaattcatggactat3000aaggaccacgacggagactacaaggatcatgatattgattacaaagacgatgacgataag3060atggccccaaagaagaagcggaaggtcggtatccacggagtcccagcagccgacaagaag3120tacagcatcggcctggacatcggcaccaactctgtgggctgggccgtgatcaccgacgag3180tacaaggtgcccagcaagaaattcaaggtgctgggcaacaccgaccggcacagcatcaag3240aagaacctgatcggagccctgctgttcgacagcggcgaaacagccgaggccacccggctg3300aagagaaccgccagaagaagatacaccagacggaagaaccggatctgctatctgcaagag3360atcttcagcaacgagatggccaaggtggacgacagcttcttccacagactggaagagtcc3420ttcctggtggaagaggataagaagcacgagcggcaccccatcttcggcaacatcgtggac3480gaggtggcctaccacgagaagtaccccaccatctaccacctgagaaagaaactggtggac3540agcaccgacaaggccgacctgcggctgatctatctggccctggcccacatgatcaagttc3600cggggccacttcctgatcgagggcgacctgaaccccgacaacagcgacgtggacaagctg3660ttcatccagctggtgcagacctacaaccagctgttcgaggaaaaccccatcaacgccagc3720ggcgtggacgccaaggccatcctgtctgccagactgagcaagagcagacggctggaaaat3780ctgatcgcccagctgcccggcgagaagaagaatggcctgttcggaaacctgattgccctg3840agcctgggcctgacccccaacttcaagagcaacttcgacctggccgaggatgccaaactg3900cagctgagcaaggacacctacgacgacgacctggacaacctgctggcccagatcggcgac3960cagtacgccgacctgtttctggccgccaagaacctgtccgacgccatcctgctgagcgac4020atcctgagagtgaacaccgagatcaccaaggcccccctgagcgcctctatgatcaagaga4080tacgacgagcaccaccaggacctgaccctgctgaaagctctcgtgcggcagcagctgcct4140gagaagtacaaagagattttcttcgaccagagcaagaacggctacgccggctacattgac4200ggcggagccagccaggaagagttctacaagttcatcaagcccatcctggaaaagatggac4260ggcaccgaggaactgctcgtgaagctgaacagagaggacctgctgcggaagcagcggacc4320ttcgacaacggcagcatcccccaccagatccacctgggagagctgcacgccattctgcgg4380cggcaggaagatttttacccattcctgaaggacaaccgggaaaagatcgagaagatcctg4440accttccgcatcccctactacgtgggccctctggccaggggaaacagcagattcgcctgg4500atgaccagaaagagcgaggaaaccatcaccccctggaacttcgaggaagtggtggacaag4560ggcgcttccgcccagagcttcatcgagcggatgaccaacttcgataagaacctgcccaac4620gagaaggtgctgcccaagcacagcctgctgtacgagtacttcaccgtgtataacgagctg4680accaaagtgaaatacgtgaccgagggaatgagaaagcccgccttcctgagcggcgagcag4740aaaaaggccatcgtggacctgctgttcaagaccaaccggaaagtgaccgtgaagcagctg4800aaagaggactacttcaagaaaatcgagtgcttcgactccgtggaaatctccggcgtggaa4860gatcggttcaacgcctccctgggcacataccacgatctgctgaaaattatcaaggacaag4920gacttcctggacaatgaggaaaacgaggacattctggaagatatcgtgctgaccctgaca4980ctgtttgaggacagagagatgatcgaggaacggctgaaaacctatgcccacctgttcgac5040gacaaagtgatgaagcagctgaagcggcggagatacaccggctggggcaggctgagccgg5100aagctgatcaacggcatccgggacaagcagtccggcaagacaatcctggatttcctgaag5160tccgacggcttcgccaacagaaacttcatgcagctgatccacgacgacagcctgaccttt5220aaagaggacatccagaaagcccaggtgtccggccagggcgatagcctgcacgagcacatt5280gccaatctggccggcagccccgccattaagaagggcatcctgcagacagtgaaggtggtg5340gacgagctcgtgaaagtgatgggccggcacaagcccgagaacatcgtgatcgaaatggcc5400agagagaaccagaccacccagaagggacagaagaacagccgcgagagaatgaagcggatc5460gaagagggcatcaaagagctgggcagccagatcctgaaagaacaccccgtggaaaacacc5520cagctgcagaacgagaagctgtacctgtactacctgcagaatgggcgggatatgtacgtg5580gaccaggaactggacatcaaccggctgtccgactacgatgtggaccatatcgtgcctcag5640agctttctgaaggacgactccatcgacaacaaggtgctgaccagaagcgacaagaaccgg5700ggcaagagcgacaacgtgccctccgaagaggtcgtgaagaagatgaagaactactggcgg5760cagctgctgaacgccaagctgattacccagagaaagttcgacaatctgaccaaggccgag5820agaggcggcctgagcgaactggataaggccggcttcatcaagagacagctggtggaaacc5880cggcagatcacaaagcacgtggcacagatcctggactcccggatgaacactaagtacgac5940gagaatgacaagctgatccgggaagtgaaagtgatcaccctgaagtccaagctggtgtcc6000gatttccggaaggatttccagttttacaaagtgcgcgagatcaacaactaccaccacgcc6060cacgacgcctacctgaacgccgtcgtgggaaccgccctgatcaaaaagtaccctaagctg6120gaaagcgagttcgtgtacggcgactacaaggtgtacgacgtgcggaagatgatcgccaag6180agcgagcaggaaatcggcaaggctaccgccaagtacttcttctacagcaacatcatgaac6240tttttcaagaccgagattaccctggccaacggcgagatccggaagcggcctctgatcgag6300acaaacggcgaaaccggggagatcgtgtgggataagggccgggattttgccaccgtgcgg6360aaagtgctgagcatgccccaagtgaatatcgtgaaaaagaccgaggtgcagacaggcggc6420ttcagcaaagagtctatcctgcccaagaggaacagcgataagctgatcgccagaaagaag6480gactgggaccctaagaagtacggcggcttcgacagccccaccgtggcctattctgtgctg6540gtggtggccaaagtggaaaagggcaagtccaagaaactgaagagtgtgaaagagctgctg6600gggatcaccatcatggaaagaagcagcttcgagaagaatcccatcgactttctggaagcc6660aagggctacaaagaagtgaaaaaggacctgatcatcaagctgcctaagtactccctgttc6720gagctggaaaacggccggaagagaatgctggcctctgccggcgaactgcagaagggaaac6780gaactggccctgccctccaaatatgtgaacttcctgtacctggccagccactatgagaag6840ctgaagggctcccccgaggataatgagcagaaacagctgtttgtggaacagcacaagcac6900tacctggacgagatcatcgagcagatcagcgagttctccaagagagtgatcctggccgac6960gctaatctggacaaagtgctgtccgcctacaacaagcaccgggataagcccatcagagag7020caggccgagaatatcatccacctgtttaccctgaccaatctgggagcccctgccgccttc7080aagtactttgacaccaccatcgaccggaagaggtacaccagcaccaaagaggtgctggac7140gccaccctgatccaccagagcatcaccggcctgtacgagacacggatcgacctgtctcag7200ctgggaggcgacaaaaggccggcggccacgaaaaaggccggccaggcaaaaaagaaaaag7260ggatcaggcaagcttgagggcagaggaagtcttctaacatgcggtgacgtggaggagaat7320cccggccctgctagcggtagcggcagcggtagcatgaccgagtacaagcccacggtgcgc7380ctcgccacccgcgacgacgtcccccgggccgtacgcaccctcgccgccgcgttcgccgac7440taccccgccacgcgccacaccgtcgacccggaccgccacatcgagcgggtcaccgagctg7500caagaactcttcctcacgcgcgtcgggctcgacatcggcaaggtgtgggtcgcggacgac7560ggcgccgcggtggcggtctggaccacgccggagagcgtcgaagcgggggcggtgttcgcc7620gagatcggcccgcgcatggccgagttgagcggttcccggctggccgcgcagcaacagatg7680gaaggcctcctggcgccgcaccggcccaaggagcccgcgtggttcctggccaccgtcggc7740gtctcgcccgaccaccagggcaagggtctgggcagcgccgtcgtgctccccggagtggag7800gcggccgagcgcgccggggtgcccgccttcctggagacctccgcgccccgcaacctcccc7860ttctacgagcggctcggcttcaccgtcaccgccgacgtcgaggtgcccgaaggaccgcgc7920acctggtgcatgacccgcaagcccggtgcctgactcgagggatccagacatgataagata7980cattgatgagtttggacaaaccacaactagaatgcagtgaaaaaaatgctttatttgtga8040aatttgtgatgctattgctttatttgtaaccattataagctgcaataaacaagttaacaa8100caacaattgcattcattttatgtttcaggttcagggggaggtgtgggaggttttttaaag8160caagtaaaacctctacaaatgtggtatggctgattatgatcagtcgaccgatgcccttga8220gagccttcaacccagtcagctccttccggtgggcgcggggcatgactatcgtcgccgcac8280ttatgactgtcttctttatcatgcaactcgtaggacaggtgccggcagcgctctgggtca8340ttttcggcgaggaccgctttcgctggagcgcgacgatgatcggcctgtcgcttgcggtat8400tcggaatcttgcacgccctcgctcaagccttcgtcactggtcccgccaccaaacgtttcg8460gcgagaagcaggccattatcgccggcatggcggccgacgcgctgggctacgtcttgctgg8520cgttcgcgacgcgaggctggatggccttccccattatgattcttctcgcttccggcggca8580tcgggatgcccgcgttgcaggccatgctgtccaggcaggtagatgacgaccatcagggac8640agcttcaaggatcgctcgcggctcttaccagccagcaaaaggccaggaaccgtaaaaagg8700ccgcgttgctggcgtttttccataggctccgcccccctgacgagcatcacaaaaatcgac8760gctcaagtcagaggtggcgaaacccgacaggactataaagataccaggcgtttccccctg8820gaagctccctcgtgcgctctcctgttccgaccctgccgcttaccggatacctgtccgcct8880ttctcccttcgggaagcgtggcgctttctcaatgctcacgctgtaggtatctcagttcgg8940tgtaggtcgttcgctccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgct9000gcgccttatccggtaactatcgtcttgagtccaacccggtaagacacgacttatcgccac9060tggcagcagccactggtaacaggattagcagagcgaggtatgtaggcggtgctacagagt9120tcttgaagtggtggcctaactacggctacactagaaggacagtatttggtatctgcgctc9180tgctgaagccagttaccttcggaaaaagagttggtagctcttgatccggcaaacaaacca9240ccgctggtagcggtggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggat9300ctcaagaagatcctttgatcttttctacggggtctgacgctcagtggaacgaaaactcac9360gttaagggattttggtcatgagattatcaaaaaggatcttcacctagatccttttaaatt9420aaaaatgaagttttaaatcaatctaaagtatatatgagtaaacttggtctgacagttacc9480aatgcttaatcagtgaggcacctatctcagcgatctgtctatttcgttcatccatagttg9540cctgactccccgtcgtgtagataactacgatacgggagggcttaccatctggccccagtg9600ctgcaatgataccgcgagacccacgctcaccggctccagatttatcagcaataaaccagc9660cagccggaagggccgagcgcagaagtggtcctgcaactttatccgcctccatccagtcta9720ttaattgttgccgggaagctagagtaagtagttcgccagttaatagtttgcgcaacgttg9780ttgccattgctgcaggcatcgtggtgtcacgctcgtcgtttggtatggcttcattcagct9840ccggttcccaacgatcaaggcgagttacatgatcccccatgttgtgcaaaaaagcggtta9900gctccttcggtcctccgatcgttgtcagaagtaagttggccgcagtgttatcactcatgg9960ttatggcagcactgcataattctcttactgtcatgccatccgtaagatgcttttctgtga10020ctggtgagtactcaaccaagtcattctgagaatagtgtatgcggcgaccgagttgctctt10080gcccggcgtcaacacgggataataccgcgccacatagcagaactttaaaagtgctcatca10140ttggaaaacgttcttcggggcgaaaactctcaaggatcttaccgctgttgagatccagtt10200cgatgtaacccactcgtgcacccaactgatcttcagcatcttttactttcaccagcgttt10260ctgggtgagcaaaaacaggaaggcaaaatgccgcaaaaaagggaataagggcgacacgga10320aatgttgaatactcatactcttcctttttcaatattattgaagcatttatcagggttatt10380gtctcatgagcggatacatatttgaatgtatttagaaaaataaacaaataggggttccgc10440gcacatttccccgaaaagtgccacctgacgtctaagaaaccattattatcatgacattaa10500cctataaaaataggcgtatcacgaggccctttcgtcttcaaggaattaattctatattct10560aaaaacacaaatgatacttctaaaaaaaatcatgaatggcatcaactctgaatcaaatct10620ttgcagatgcacctacttctcatttccactgtcacatcatttttcc10666<210>11<211>15<212>dna<213>人工序列(artificialsequence)<400>11atgcttataacggag15<210>12<211>87<212>dna<213>人工序列(artificialsequence)<400>12agctcgccgccgtcgccgccgggcgacgagaaggagtccaagatgagccagatacagttc60tcacagcccttcgacgaggacgcctcg87<210>13<211>119<212>dna<213>人工序列(artificialsequence)<400>13gtgtggtcgaagcggcaacaggcggtgtgcgtgagacacgcctacaagcactacggctcc60aacaagcggcccaaccacgtgctcagcaacctcaacatgaccgtcgccaaggggaccat119<210>14<211>119<212>dna<213>人工序列(artificialsequence)<400>14ctacggtctgctgggcgcgtcgggctgcggcaagacgacgctgctgtcgtgcatcgtggg60gcgccgcaagctcaacagcggggagatctgggtgctgggcggcaagcccgggactaaag119<210>15<211>196<212>dna<213>人工序列(artificialsequence)<400>15gctcgggcgtgccgggcaagcgcgtgggctacatgccgcaggagatcgcgctgtacggag60agttcaccatcaaggagaccatgatgtacttcggctggatcttcggcatgaagacgtcgg120agatcctggagcggctgcgcttcctgctggacttcctcgacctgcccagcgagaacagga180tggtcaagaacctcag196<210>16<211>202<212>dna<213>人工序列(artificialsequence)<400>16cggcggtcagtcgcggcgcgtgtcgttcgcggtggcgctgatgcacgaccccgagctgct60gatcctggacgagcccacggtgggcgtggacccgctgctgcgccagtccatctggacgca120cctcgtgcgcatcaccggcgccgccgacaagaccgtcatcatcaccacgcactacatcga180ggaggcgcgccaggcgcacacc202<210>17<211>115<212>dna<213>人工序列(artificialsequence)<400>17ataggtctgatgcggagcgggcggctgctggcggagcagtctcctcaggcgctgctgcag60atgtacaactgcatctcactcgaagacgtcttcctcaagctgtcccggaaacagg115<210>18<211>176<212>dna<213>人工序列(artificialsequence)<400>18caggtcagtccaaccaggcggtagagctccaagtggcgggcgggctcgggctcaccaagc60tgtcgcggcgcgaggaggcgccctacgcccacgaggatgcgcacgtcaaggggctcaact120tccaccagagcaaggaggtgctcatcacggaccacaatggcgttagtaatggcgat176<210>19<211>63<212>dna<213>人工序列(artificialsequence)<400>19attcccggcaagatcgcgccgagcatgaaggccgactgtgatgactgcggagactgcttg60agt63<210>20<211>168<212>dna<213>人工序列(artificialsequence)<400>20ccactcctgaatttcacctcccgagggaagatcaaggcgctgatccagaagaacttcctg60cgcatgtggaggaacatcggggtgatgctgttcatcttcgttctgcccgtcatgcaggtc120atcctgttctgtctcgccatcggacgggaccccgtcgggctgagactg168<210>21<211>235<212>dna<213>人工序列(artificialsequence)<400>21gcgatagtaaacaacgacgtgaacgtagtggacggctactgtccgtacaacaacacgtgc60tccatgaagaacctgtcctgccgctacctcgaccacctcaagaaccagtccatcatcaag120gagtactacgccgacctcgaaagcgccatcaacgccgtcaaggaggggcaggcgtggggc180gccatctacttcaatgagaattatacagactctctggtcgcgagactggctttgg235<210>22<211>125<212>dna<213>人工序列(artificialsequence)<400>22ctgacactgcggacgacgagaccatcgagtcgtcagaagtgcaggtgtggctcgacatgt60ccaaccagcagatcggcctcatgctcaaccgagacatacagttctcatacagggacttcg120ctaag125<210>23<211>125<212>dna<213>人工序列(artificialsequence)<400>23aacttgctgtcgacttgcgactacaacccgaaggtgggcgacatcccgatcgacttcaag60gagcccatctacggcgacaccaacccctccttcactgacttcgtcgctcccggtgttatt120ctcac125<210>24<211>245<212>dna<213>人工序列(artificialsequence)<400>24tatcgtgttcttcctggcggtggcgctgacgtcgtcggcgctgatcgtggagcgcatgga60ggggctgctggaccgctcgtgggtggccggcgtgtcccccggggagatcctgttctcgca120cgtcgtcacgcagttcgtcgtcatgtgcggccagaccgcgctcgtgctcgtgttcatgat180actggtgttcggcgtcaagtgtaacgggaatatcttctttgtggtcctgttgacgctgct240gcaag245<210>25<211>323<212>dna<213>人工序列(artificialsequence)<400>25gtctgtgcgggatgtgcttcggcttcgtaatctccgccgcgtgcgaactggaacgaaacg60cgatccagctagcactcggctcattctaccccacgcttcttctgagcggggtgatctggc120ccatcgagggcatgccgtggatcctgcgctacatctccacgtgtctgccgctgactctgg180cgacctcctcgctgcggtccatcctgacccgcggctggcccatcaccgaccccgaggtct240acatgggcttcatctccaccctcatctggatcgctctgttcctcgtagtcaccatcacag300ttctaaggttcaagaaaggataa323当前第1页12