本发明属于基因组编辑技术与代谢工程领域,具体涉及提高枯草芽孢杆菌crispr-cas9n系统基因组多位点编辑效率的方法及在代谢工程中应用。
背景技术:
高效的基因组编辑工具是代谢网络调控的关键。随着系统生物学的发展和开发微生物细胞工厂的需要,对代谢网络的优化调控已经逐渐从对单一基因或途径的调控发展为对多靶点、多途径的同时修饰。因此,多重基因组编辑工具的开发是基因编辑技术领域的重要内容。
以具有切口酶活性的cas9n为基础的crispr-cas9n基因组编辑技术克服了cas9系统对宿主细胞生长压力大,双链切口不利于修复且脱靶率高的缺点。同时在多位点基因组编辑时,单链切口不会过多的破坏基因组的完整性,因此有更高的编辑效率。
目前枯草芽孢杆菌传统基因组编辑技术中,无法实现高效的2位点以上基因编辑。此外,近年来随着cirspr技术在基因编辑领域的广泛应用,显著提高了枯草芽孢杆菌的基因编辑效率。目前枯草芽孢杆菌crispr-cas9n系统多位点编辑效率较低。
技术实现要素:
本发明的目的是克服现有技术的不足,提供一种提高枯草芽孢杆菌crispr-cas9n系统基因组多位点编辑效率的方法。
本发明的第二个目的是提供一种利用枯草芽孢杆菌crispr-cas9n基因组编辑系统,提升枯草芽孢杆菌bs89的核黄素生产能力。
本发明的技术方案概述如下:
提高枯草芽孢杆菌crispr-cas9n基因组编辑系统多位点编辑效率的方法,包括如下步骤:
(1)根据编辑位点,构建枯草芽孢杆菌crispr-cas9n基因组编辑系统;
(2)敲除待编辑枯草芽孢杆菌菌株的ligd基因,所述ligd基因的核苷酸序列如seqidno.90所示;
(3)将步骤(1)获得的枯草芽孢杆菌crispr-cas9n基因组编辑系统导入步骤(2)获得的重组菌中进行基因组多位点编辑。
利用枯草芽孢杆菌crispr-cas9n基因组编辑系统,提升枯草芽孢杆菌bs89的核黄素生产能力,包括如下步骤:
(1)对枯草芽孢杆菌bs89核黄素操纵子上的riba,ribb,ribh三个基因的rbs区域进行同时修饰,根据三个修饰位点构建枯草芽孢杆菌crispr-cas9n基因组编辑系统,所述riba基因的核苷酸序列如seqidno.47所示;所述ribb基因的核苷酸序列如seqidno.48所示;所述ribh基因的核苷酸序列如seqidno.49所示;
(2)敲除枯草芽孢杆菌bs89菌株的ligd基因,所述ligd基因的核苷酸序列如seqidno.90所示;
(3)将步骤(1)获得的crispr-cas9n基因组编辑系统导入步骤(2)获得的重组菌中,对核黄素操纵子上的riba,ribb,ribh三个基因的rbs区域进行随机突变,获得不同突变组合的菌株,从中筛选到核黄素生产能力显著提升的菌株。
本发明的优点:
本发明的方法可以提高枯草芽孢杆菌crispr-cas9n系统基因组多位点编辑效率。
利用枯草芽孢杆菌crispr-cas9n基因组编辑系统,提升枯草芽孢杆菌bs89的核黄素生产能力。
附图说明
图1为三位点编辑效率对比。
图2为核黄素操纵子优化菌株96孔板发酵结果。
图3为核黄素操纵子优化菌株摇瓶复筛结果。
具体实施方式
下面结合实施例对本发明做进一步说明,下述实施例是为了使本领域的技术人员能够更好地理解本发明,但对本发明不作任何限制。
本发明所用到的宿主菌株bacillussubtilis168来源为bgsc(bacillusgeneticstockcenter,http://www.bgsc.org/)。枯草芽孢杆菌bs89来源为参考文献shit,wangy,wangz,wangg,liud,fuj,chent,zhaox.deregulationofpurinepathwayinbacillussubtilisanditsuseinriboflavinbiosynthesis.microbialcellfactories.2014,13(1):101.
本发明所用到的原始质粒pax01和php13来源为bgsc(bacillusgeneticstockcenter,http://www.bgsc.org/)。
质粒pebs-cop1来源于专利《枯草芽孢杆菌基因组无痕修饰方法》(申请公布专利号:cn103451224a)。
质粒puc18和质粒pcas9cur来源于addgene(http://www.addgene.org)。
pgrb质粒来源于参考文献liet.al.metabolicengineeringofescherichiacoliusingcrispr-cas9meditatedgenomeediting.metabeng.2015,31:13-21.
所用限制性内切酶、去磷酸化酶、dna连接酶等分子生物学试剂从thermo公司购买(http://www.thermoscientificbio.com/fermentas),所用其他生化试剂从生工生物工程(上海)股份有限公司购买(http://www.sangon.com/)。
实施例1构建枯草芽孢杆菌crispr-cas9n基因组编辑系统
本发明所用到的质粒pbscas9n以b.subtilis168基因组为模板,用引物p43-f和p43-l扩增p43启动子序列,以质粒puc18为模板,用引物cole1-f和cole1-l扩增puc18复制子cole1序列,用引物p43-f和cole1-l将以上两片段融合为片段f1。这一步融合中,通过引物在p43启动子下游引入grna与cas9蛋白结合的序列和终止子序列。以质粒php13为模板,用引物cm-f和cm-l扩增氯霉素抗性基因及其启动子序列(cm),以质粒pebs-cop1为模板,用引物repf-f和repf-l扩增pebs-cop1复制子repf序列,以质粒pax01为模板,引物xyla-f和xyla-l扩增木糖启动子及诱导元件序列(xylacassette),用引物cm-f和xyla-l将以上三片段融合为片段f2。以pcas9cur为出发质粒用引物cas9-f和cas9-l扩增cas9基因序列。将获得的f1、f2和cas9片段测定浓度后,按等摩尔比例加入cpecpcr反应体系中进行一步组装,最终得到pbscas9质粒。
通过对pbscas9质粒上cas9蛋白的内切酶区域进行点突变的方式获得cas9n序列。以pbscas9为模板,采用5’端磷酸化引物d10-f和d10-l全质粒扩增pbscas9,通过引物引入点突变,将原cas9序列中第10位asp(gat)突变为ala(gct),得到cas9n;全质粒扩增后的片段经t4连接酶连接、转化、筛选阳性克隆,得到质粒pbscas9n。
所述cas9基因核苷酸序列如seqidno.76所示;氯霉素抗性基因及其启动子的核苷酸序列如seqidno.77所示;质粒pebs-cop1的复制子的核苷酸序列如seqidno.78所示;质粒puc18的复制子的核苷酸序列如seqidno.79所示;枯草芽孢杆菌启动子p43的核苷酸序列如seqidno.80所示;质粒pax01上的木糖启动子及诱导元件的核苷酸序列如seqidno.81所示;所述质粒pbscas9的核苷酸序列如seqidno.82所示;所述质粒pbscas9n的核苷酸序列如seqidno.83所示。
以枯草芽孢杆菌质粒php13为出发质粒构建pdonor质粒。
采用引物manp-f和manp-l对质粒php13进行第一轮全质粒扩增,扩增过程中通过引物引入启动子pmanp序列,通过goldengate克隆重新连接质粒,获得质粒php13-2。采用引物grna-f和grna-l对php13-2质粒进行全质粒扩增,扩增过程中通过引物引入靶向复制子rep60的grna序列grna-rep60,通过goldengate克隆重新连接质粒,获得质粒php13-3。最后将质粒上的氯霉素基因及启动子序列替换为氨苄青霉素基因及启动子序列,以pgrb质粒为模板,采用引物amp-f和amp-l扩增氨苄青霉素基因及启动子序列。以php13-3为模板,采用引物p3-f和p3-l扩增模板质粒,以上两段pcr产物通过goldengate克隆连接,获得质粒pdonor。
所述枯草芽孢杆菌启动子pmanp的核苷酸序列如seqidno.84所示;所述靶向复制子rep60的grna的核苷酸序列如seqidno.85所示;所述氨苄青霉素抗性基因及启动子核苷酸序列如seqidno.86所示,所述质粒pdonor的核苷酸序列如seqidno.87所示;
工具质粒phg的构建
以质粒puc18为模板,采用引物puc-f和puc-l扩增复制子(cole1)和氨苄青霉素基因(amp)组合区域序列。以b.subtilis168基因组为模板,采用引物p-f和p-l扩增p43启动子序列。以上两个pcr片段,通过cpec进行一步组装,获得phg质粒。在质粒的构建过程中,grna与cas9蛋白结合的序列和终止子序列通过引物分别引入。
所述质粒puc18的复制子(cole1)和氨苄青霉素抗性基因及其启动子(amp)组合区域核苷酸序列如seqidno.88所示,所述枯草芽孢杆菌启动子p43的核苷酸序列如seqidno.80所示,多位点grna表达过程中辅助质粒phg的核苷酸序列如seqidno.89所示。
由质粒pbscas9n,pdonor和phg组成枯草芽孢杆菌crispr-cas9n基因组编辑系统。
实施例2枯草芽孢杆菌ligd基因的失活
以枯草芽孢杆菌bacillussubtilis168为出发菌株,敲除ligd基因(seqidno.90)。此过程采用crispr-cas9n系统完成。以质粒pbscas9n为模板,采用引物ligdgrna-f和ligdgrna-l进行全质粒pcr扩增,上下游引物中分别引入bsai酶切位点以及一部分grna序列,所得片段通过goldengate克隆重新连接,此过程中将grna序列插入pbscas9n质粒p43启动子下游区域。以bacillussubtilis168基因组为模板,采用引物donligdu-f/l和donligdd-f/l分别扩增上下游同源臂片段,以pdonor质粒为模板,采用引物pdonorligd-f/l进行全质粒扩增,所得pcr产物和上下游同源臂片段通过cpec反应重新连接,此过程中将同源臂片段组装至质粒pdonor。敲除ligd基因具体流程为:
(1)依次将上述为敲除ligd构建的pbscas9n和pdonor质粒转入宿主菌株,最终获得带有双质粒系统的菌株。
(2)将带有双质粒系统的阳性克隆菌株接入氯霉素(5μg/ml)和红霉素(0.5μg/ml)双抗且1%木糖终浓度的lb液体培养基中,培养8h,培养过程中木糖诱导cas9n切割基因组提升同源重组效率。
(3)木糖诱导培养后的菌液经稀释,涂布于氯霉素和红霉素双抗性的lb平板,获得一定数量的单菌落,待进一步验证。
(4)采用dna测序验证ligd是否敲除成功。
(5)将验证正确的菌株接种于终浓度1%木糖和1%甘露糖的氯霉素(5μg/ml)lb液体培养基中,过夜培养,将菌液在氯霉素平板上划线,培养至菌落可见。
(6)将氯霉素平板上的单菌落对点于红霉素(0.5μg/ml)平板,不能在红霉素平板上生长的菌落是pdonor质粒消除的菌株,为保证成功率,可一次对点多个菌落以确保获得质粒消除的菌株。
(7)将pdonor质粒消除的菌株,接种在无抗lb液体培养基中,50℃培养至少8h,将菌液在无抗lb平板上划线,获得的菌落对点于氯霉素(5μg/ml)平板,在氯霉素平板上不能生长的菌落为pbscas9n质粒消除的菌株。
将两个质粒消除后,最终获得失活基因ligd的菌株bacillussubtilis168δligd。
实施例3:敲除ligd提高crispr-cas9n基因组编辑系统多位点编辑效率
选取amye,upp和sige三个基因位点来表征crispr-cas9n多位点编辑效率。基因编辑方式为同时将以上三个基因中连续两个密码子突变为终止密码子。确定三个基因位点中的三条grna序列为:
grna-1:gcggtgatagcttctcgttc(seqidno.91)
grna-2:gctgattcctgcggcaaaag(seqidno.92)
grna-3:gaagctcccaaacggcgatc(seqidno.43)
以pbscas9n为模板,采用引物tm1-f/l进行全质粒扩增,以phg为模板,引物tm2-f/l和tm3-f/l分别扩增质粒上的目的片段。上述引物中分别引入bsai酶切位点和相应的部分grna序列,上述得到的三条pcr片段通过goldengate克隆重新连接,获得将三条grna序列及其对应启动子分别插入的pbscas9n质粒。以bacillussubtilis168基因组为模板,采用引物thrmuamyeu-f/l和thrmuamyed-f/l扩增amye位点处的上下游同源臂;采用引物thrmuuppu-f/l和thrmuuppd-f/l扩增upp位点处的上下游同源臂;采用引物thrmusigeu-f/l和thrmusigeu-f/l扩增sige位点处的上下游同源臂。在上下游同源臂构建过程中引入点突变,分别将三个基因pam位点附近的连续两个密码子突变为终止密码子。将上述三个基因各自的上下游同源臂通过融合pcr融合为一段同源臂序列。以pdonor质粒为模板,采用引物pdonthrmu-f/l进行全质粒扩增,所得pcr产物与上述三个位点融合后的同源臂片段通过cpec重新连接,此过程中将三个位点的同源臂片段均插入质粒pdonor中。
构建质粒后,分别对bacillussubtilis168和bacillussubtilis168δligd进行三位点同时修饰,并比较其效率。具体操作流程为
(1)依次将上述为修饰三位点构建的pbscas9n和pdonor质粒转入宿主菌株,最终获得带有双质粒系统的菌株。
(2)将带有双质粒系统的阳性克隆菌株接入氯霉素(5μg/ml)和红霉素(0.5μg/ml)双抗且1%木糖终浓度的lb液体培养基中,培养8h,培养过程中木糖诱导cas9n切割基因组提升同源重组效率。
(3)木糖诱导培养后的菌液经稀释,涂布于氯霉素和红霉素双抗性的lb平板,获得一定数量的单菌落,待进一步验证突变效率。
(4)采用dna测序验证发生突变的阳性克隆,并根据验证的总菌落数计算突变效率。
经比较不同菌株三位点编辑效率,ligd失活菌株中的三位点编辑效率明显提高(图1)。
实施例4:利用枯草芽孢杆菌crispr-cas9n基因组编辑系统,提升枯草芽孢杆菌bs89的核黄素生产能力
对枯草芽孢杆菌bs89的核黄素操纵子上riba,ribb和ribh三个基因的rbs区域进行随机突变,获得不同突变组合的菌株,从中筛选到核黄素生产能力显著提升的菌株。
riba基因的核苷酸序列如seqidno.47所示;
ribb基因的核苷酸序列如seqidno.48所示;
ribh基因的核苷酸序列如seqidno.49所示;
具体流程为:
依据实施例2中的方法,失活枯草芽孢杆菌核黄素生产菌株bs89的ligd基因,得到菌株bs89δligd。
采用crispr-cas9n多位点编辑系统,在ligd失活的枯草芽孢杆菌核黄素生产菌株中优化核黄素操纵子,设计简并引物构建rbs文库,对ribb,riba和ribh三个基因的rbs序列同时突变,通过组合优化筛选得到核黄素合成能力显著提高的菌株。
(1)构建相应的crispr-cas9n基因组编辑系统。确定三个基因位点的grna序列为:
grna-riba:gatagcggtaccattataaa(seqidno.44)
grna-ribb:aaaccgacaaaggaatagga(seqidno.45)
grna-ribh:aatcacaaatatcacaaaaa(seqidno.46)
以pbscas9n为模板,采用引物grib1-f/l,进行全质粒扩增,以phg为模板,引物grib2-f/l和grib3-f/l分别扩增质粒上的目的片段。上述得到的三条pcr片段通过goldengate克隆重新连接,获得将三条grna序列及其相应启动子插入的pbscas9n质粒。以bacillussubtilis168基因组为模板,采用引物ribbu-f/l和ribbd-f/l扩增ribb位点处的上下游同源臂;采用引物ribau-f/l和ribad-f/l扩增riba位点处的上下游同源臂;采用引物ribhu-f/l和ribhd-f/l扩增ribh位点处的上下游同源臂。其中引物ribbd-f,ribad-f和ribhd-f均为简并引物,获得的同源臂在同源重组过程中可对相依基因位点引入随机突变。以pdonor质粒为模板,采用引物pdonlib-f/l进行全质粒扩增,所得pcr产物与上述三个位点的同源臂片段通过cpec重新连接,将三个位点处的上下游同源臂序列插入pdonor中。
(2)构建crispr-cas9n系统后,采用与实施例2中相同的方法敲除枯草芽孢杆菌bs89菌株的ligd基因(seqidno.90)。以质粒pbscas9n为模板,采用引物ligdgrna-f和ligdgrna-l进行全质粒pcr扩增,上下游引物中分别引入bsai酶切位点以及一部分grna序列,所得片段通过goldengate克隆重新连接,此过程中将grna序列插入pbscas9n质粒p43启动子下游区域。以bs89基因组为模板,采用引物donligdu-f/l和donligdd-f/l分别扩增上下游同源臂片段,以pdonor质粒为模板,采用引物pdonorligd-f/l进行全质粒扩增,所得pcr产物和上下游同源臂片段通过cpec反应重新连接,此过程中将同源臂片段组装至质粒pdonor。敲除ligd基因具体流程为:
1)依次将上述为敲除ligd构建的pbscas9n和pdonor质粒转入宿主菌株,最终获得带有双质粒系统的菌株。
2)将带有双质粒系统的阳性克隆菌株接入氯霉素(5μg/ml)和红霉素(0.5μg/ml)双抗且1%木糖终浓度的lb液体培养基中,培养8h,培养过程中木糖诱导cas9n切割基因组提升同源重组效率。
3)木糖诱导培养后的菌液经稀释,涂布于氯霉素和红霉素双抗性的lb平板,获得一定数量的单菌落,待进一步验证。
4)采用dna测序验证ligd是否敲除成功。
5)将验证正确的菌株接种于终浓度1%木糖和1%甘露糖的氯霉素(5μg/ml)lb液体培养基中,过夜培养,将菌液在氯霉素平板上划线,培养至菌落可见。
6)将氯霉素平板上的单菌落对点于红霉素(0.5μg/ml)平板,不能在红霉素平板上生长的菌落是pdonor质粒消除的菌株,为保证成功率,可一次对点多个菌落以确保获得质粒消除的菌株。
7)将pdonor质粒消除的菌株,接种在无抗lb液体培养基中,50℃培养至少8h,将菌液在无抗lb平板上划线,获得的菌落对点于氯霉素(5μg/ml)平板,在氯霉素平板上不能生长的菌落为pbscas9n质粒消除的菌株。
将两个质粒消除后,最终获得失活基因ligd的菌株bs89δligd。
(3)将步骤(1)中构建的两个质粒分别转入bs89δligd,将带有双质粒系统的阳性克隆菌株接入氯霉素(5μg/ml)和红霉素(0.5μg/ml)双抗且1%木糖终浓度的lb液体培养基中,培养8h,木糖诱导cas9n表达,菌液经稀释涂布于氯霉素和红霉素双抗性的lb平板,获得不同rbs突变组合的菌株文库。
首先通过颜色辨别尽量挑取黄色较深的菌落,重新对点于新的平板中,作为待发酵菌株文库。将平板上的菌落逐个接种于96深孔板中培养,41℃,发酵24h。发酵结束后分别测定每株菌的核黄素产量,bs89菌株作为对照。整个过程中共筛选菌株190株,发酵验证的190株菌株中,111株菌株在初筛中产量超过了bs89(图2)。综合选取有潜力的初筛菌株20株进行摇瓶发酵复筛,在500ml摇瓶中发酵48h(图3),其中18株菌株的摇瓶发酵产量超过bs89,摇瓶发酵中产量最高的4株菌株cy124、cy79、cy49和cy46在初筛中均为较高产量菌株。两次筛选具有较好的一致性。cy46为复筛最高产量菌株,其在复筛产量最高的4株菌株中,初筛时的产量也最高。cy46产量达到1.39g/l,得率为17.16mg/g葡萄糖。其产量与对照菌株bs89相比,提高了59%。对产量最高的4株菌株核黄素操纵子区域进行dna测序,4株菌株的三个rbs位点均发生了突变(表2),证明通过ligd基因的失活,crispr-cas9n编辑系统的多位点编辑效率提高,高效率的对核黄素操纵子上不同基因的表达水平进行了组合优化,进而提升了核黄素的合成能力。
96孔板发酵与摇瓶发酵所用培养基为ye培养基,ye培养基配方为:葡萄糖100g/l,酵母粉(ye)20g/l,mgso4·7h2o0.5g/l,kh2po40.5g/l,k2hpo40.5g/l。
表1质粒构建所用引物序列
表2组合优化菌株核黄素操纵子基因rbs区域序列
序列表
<110>天津大学
<120>提高枯草芽孢杆菌crispr-cas9n系统基因组多位点编辑效率的方法
<160>92
<170>siposequencelisting1.0
<210>1
<211>51
<212>dna
<213>人工序列(artificialsequence)
<400>1
cacaccaggtctcactgtctcgcagttttagagctagaaatagcaagttaa51
<210>2
<211>59
<212>dna
<213>人工序列(artificialsequence)
<400>2
cacaccaggtctcaacagaggcaatcgggtgtacattcctctcttacctataatggtac59
<210>3
<211>45
<212>dna
<213>人工序列(artificialsequence)
<400>3
gttttacaacgtcgtgatgcgctcaaccggaaagatcggtatcgc45
<210>4
<211>50
<212>dna
<213>人工序列(artificialsequence)
<400>4
acacggaaagagctgacttcattaggcgctatgatgtgccggaggctttt50
<210>5
<211>50
<212>dna
<213>人工序列(artificialsequence)
<400>5
aaaagcctccggcacatcatagcgcctaatgaagtcagctctttccgtgt50
<210>6
<211>40
<212>dna
<213>人工序列(artificialsequence)
<400>6
acgccagggttttcccaggcgttatccggaagcgaatctg40
<210>7
<211>40
<212>dna
<213>人工序列(artificialsequence)
<400>7
cagattcgcttccggataacgcctgggaaaaccctggcgt40
<210>8
<211>36
<212>dna
<213>人工序列(artificialsequence)
<400>8
atctttccggttgagcgcatcacgacgttgtaaaac36
<210>9
<211>48
<212>dna
<213>人工序列(artificialsequence)
<400>9
cacaccaggtctcactcgttcgttttagagctagaaatagcaagttaa48
<210>10
<211>61
<212>dna
<213>人工序列(artificialsequence)
<400>10
cacaccaggtctcagccgtttgggagcttcgtgtacattcctctcttacctataatggta60
c61
<210>11
<211>51
<212>dna
<213>人工序列(artificialsequence)
<400>11
cacaccaggtctcagcggcaaaaggttttagagctagaaatagcaagttaa51
<210>12
<211>62
<212>dna
<213>人工序列(artificialsequence)
<400>12
cacaccaggtctcacgagaagctatcaccgcgtgtacattcctctcttacctataatggt60
ac62
<210>13
<211>49
<212>dna
<213>人工序列(artificialsequence)
<400>13
cacaccaggtctcacggcgatcgttttagagctagaaatagcaagttaa49
<210>14
<211>59
<212>dna
<213>人工序列(artificialsequence)
<400>14
cacaccaggtctcaccgcaggaatcagcgtgtacattcctctcttacctataatggtac59
<210>15
<211>44
<212>dna
<213>人工序列(artificialsequence)
<400>15
gttttacaacgtcgtgatctgatcgatgggatgtcacgcagaat44
<210>16
<211>33
<212>dna
<213>人工序列(artificialsequence)
<400>16
gaggcgtactttattaacgagaagctatcaccg33
<210>17
<211>33
<212>dna
<213>人工序列(artificialsequence)
<400>17
cggtgatagcttctcgttaataaagtacgcctc33
<210>18
<211>53
<212>dna
<213>人工序列(artificialsequence)
<400>18
gctacacgctgtcttgcttcttcaagttgataaacggcttttgttgtattcgc53
<210>19
<211>53
<212>dna
<213>人工序列(artificialsequence)
<400>19
gcgaatacaacaaaagccgtttatcaacttgaagaagcaagacagcgtgtagc53
<210>20
<211>29
<212>dna
<213>人工序列(artificialsequence)
<400>20
catgtccttattatgccgcaggaatcagc29
<210>21
<211>29
<212>dna
<213>人工序列(artificialsequence)
<400>21
gctgattcctgcggcataataaggacatg29
<210>22
<211>54
<212>dna
<213>人工序列(artificialsequence)
<400>22
gctgttcgatggcttccaatgggtcgcccctaacatgtgtcaatctgtttaaag54
<210>23
<211>54
<212>dna
<213>人工序列(artificialsequence)
<400>23
ctttaaacagattgacacatgttaggggcgacccattggaagccatcgaacagc54
<210>24
<211>31
<212>dna
<213>人工序列(artificialsequence)
<400>24
gcgcgcttattaatcgccgtttgggagcttc31
<210>25
<211>31
<212>dna
<213>人工序列(artificialsequence)
<400>25
gaagctcccaaacggcgattaataagcgcgc31
<210>26
<211>45
<212>dna
<213>人工序列(artificialsequence)
<400>26
acgccagggttttcccaggaaatccccatcatatccgctacatcc45
<210>27
<211>45
<212>dna
<213>人工序列(artificialsequence)
<400>27
ggatgtagcggatatgatggggatttcctgggaaaaccctggcgt45
<210>28
<211>44
<212>dna
<213>人工序列(artificialsequence)
<400>28
attctgcgtgacatcccatcgatcagatcacgacgttgtaaaac44
<210>29
<211>61
<212>dna
<213>人工序列(artificialsequence)
<400>29
gttatcaacttgaaaaagtggcacccaggatgctcagcaatacaggaaaacacaccaaag60
c61
<210>30
<211>30
<212>dna
<213>人工序列(artificialsequence)
<400>30
ctattcctttgtcggttttgccgtcagttt30
<210>31
<211>59
<212>dna
<213>人工序列(artificialsequence)
<400>31
caaaaccgacaaaggaatagrnnnnrraattcaaaatgtttacaggaattatcgaagaa59
<210>32
<211>27
<212>dna
<213>人工序列(artificialsequence)
<400>32
ctaaaagccgttttcgcttaagaaggc27
<210>33
<211>27
<212>dna
<213>人工序列(artificialsequence)
<400>33
gccttcttaagcgaaaacggcttttag27
<210>34
<211>27
<212>dna
<213>人工序列(artificialsequence)
<400>34
tataatggtaccgctatcactttatat27
<210>35
<211>59
<212>dna
<213>人工序列(artificialsequence)
<400>35
gtgatagcggtaccattatarnnnnrraattcaaaatgtttcatccgatagaagaagca59
<210>36
<211>31
<212>dna
<213>人工序列(artificialsequence)
<400>36
aatgcacccggaccaataccggttcttctcc31
<210>37
<211>31
<212>dna
<213>人工序列(artificialsequence)
<400>37
ggagaagaaccggtattggtccgggtgcatt31
<210>38
<211>30
<212>dna
<213>人工序列(artificialsequence)
<400>38
ttagaaatgaagtaaatgacctagcttgtt30
<210>39
<211>59
<212>dna
<213>人工序列(artificialsequence)
<400>39
ggtcatttacttcatttctaarnnnnrraattcaaaatgaatatcatacaaggaaattt59
<210>40
<211>58
<212>dna
<213>人工序列(artificialsequence)
<400>40
gacgttgtaaaacgacggccagtgaaaaaaacccctctatatccaaaatgtccagtct58
<210>41
<211>58
<212>dna
<213>人工序列(artificialsequence)
<400>41
agactggacattttggatatagaggggtttttttcactggccgtcgttttacaacgtc58
<210>42
<211>61
<212>dna
<213>人工序列(artificialsequence)
<400>42
gctttggtgtgttttcctgtattgctgagcatcctgggtgccactttttcaagttgataa60
c61
<210>43
<211>20
<212>dna
<213>人工序列(artificialsequence)
<400>43
gaagctcccaaacggcgatc20
<210>44
<211>20
<212>dna
<213>人工序列(artificialsequence)
<400>44
gatagcggtaccattataaa20
<210>45
<211>20
<212>dna
<213>人工序列(artificialsequence)
<400>45
aaaccgacaaaggaatagga20
<210>46
<211>20
<212>dna
<213>人工序列(artificialsequence)
<400>46
aatcacaaatatcacaaaaa20
<210>47
<211>1197
<212>dna
<213>枯草芽孢杆菌(bacillussubtilis)
<400>47
atgtttcatccgatagaagaagcactggacgctttaaaaaaaggcgaagtcatcatcgtt60
gtagatgatgaagacagagaaaatgaaggagactttgtggctcttgccgagcatgcaacg120
ccggaagtcattaactttatggcgacacatgggagaggactgatctgcacgccgctcagt180
gaggaaatcgcagacaggcttgatcttcaccctatggttgagcataatacagactctcac240
cacactgcatttaccgtaagcatagaccatcgtgaaacgaagacaggtatcagcgctcaa300
gaaagatcttttaccgttcaagcattgctggacagcaaatccgtgccatctgattttcag360
cgtccggggcacatttttccactgattgcgaaaaaaggaggtgtcctgaaaagagcgggc420
catacagaagctgctgttgatcttgctgaagcttgcggatctccaggagccggcgtcatt480
tgtgaaattatgaatgaagacggaacgatggcgagagtgcctgagctcattgaaattgcg540
aaaaagcatcaattaaaaatgatcaccattaaggatttgattcaataccgttacaatctg600
acaacacttgtcgagcgtgaagttgacattacgctgcctactgattttgggacatttaag660
gtttatggatacacaaatgaggtagatggaaaagagcatgtcgcatttgtgatgggagat720
gtgccgttcggagaagaaccggtattggtccgggtgcattcagaatgtctcacaggtgac780
gtgtttggctctcatcgctgtgattgcggaccgcagctgcacgccgcgctgaaccaaatt840
gccgcagaaggccgtggagtgctcctgtacttgcgccaagaaggacgaggcatcggttta900
atcaataaattaaaagcttataagcttcaggaacaaggctatgacaccgtagaagccaat960
gaggcgcttggattcttgccggatcttcgcaactatggcatcggagcacaaattttacgc1020
gacctcggtgtccggaatatgaagcttttgacgaataatccgcgaaaaatcgcaggcctt1080
gaaggctacggactcagtatttcagaaagagtgccgcttcaaatggaggcgaaagaacac1140
aataaaaaatatttgcaaaccaaaatgaacaagctaggtcatttacttcatttctaa1197
<210>48
<211>648
<212>dna
<213>枯草芽孢杆菌(bacillussubtilis)
<400>48
atgtttacaggaattatcgaagaaacaggcacaatcgaatccatgaaaaaagcagggcat60
gcaatggccttaactattaaatgctcaaagattttagaggatgttcatcttggcgacagc120
attgcagtgaacggcatttgtctgactgtcactgattttacaaaaaatcaattcacagtg180
gatgttatgcctgaaacagtcaaagctacgtcactgaatgatttaacaaaaggaagcaaa240
gtaaatctggaaagagcgatggcggcaaacggccgtttcggaggccatttcgtctcaggc300
catgtcgacggaactgcggaaatcacacgaattgaagagaaaagcaacgcagtttactat360
gatttaaaaatggacccgtcattaacaaaaacattggttttaaagggatcaattactgtg420
gatggcgtgagcttaaccatattcggcctgacagaagacacagtgacgatctccttaata480
ccgcatacgatcagcgaaacgatcttttcagaaaaaacgatcggctctaaagtgaatatc540
gaatgcgatatgatcggaaaatatatgtatcgatttttgcataaagccaatgaaaataag600
acccaacaaaccattacaaaagccttcttaagcgaaaacggcttttag648
<210>49
<211>465
<212>dna
<213>枯草芽孢杆菌(bacillussubtilis)
<400>49
atgaatatcatacaaggaaatttagttggtacaggtcttaaaatcggaatcgtagtagga60
agatttaatgattttattacgagcaagctgctgagcggagcagaagatgcgctgctcaga120
catggcgtagacacaaatgacattgatgtggcttgggttccaggcgcatttgaaataccg180
tttgctgcgaaaaaaatggcggaaacaaaaaaatatgatgctattatcacattgggcact240
gtcatcagaggcgcaacgacacattacgattatgtctgcaatgaagctgcaaaaggcatc300
gcgcaagcagcaaacactactggtgtacctgtcatctttggaattgtaacaactgaaaac360
atcgaacaggctatcgagcgtgccggcacaaaagcgggcaacaaaggtgtagattgtgct420
gtttctgccattgaaatggcaaatttaaaccgctcatttgaataa465
<210>50
<211>56
<212>dna
<213>人工序列(artificialsequence)
<400>50
cacgcattgatttgagtcagctaggaggtgactgacgtgcatgcaggccggggcat56
<210>51
<211>35
<212>dna
<213>人工序列(artificialsequence)
<400>51
gtcatcaccgaaacgtgtacattcctctcttacct35
<210>52
<211>58
<212>dna
<213>人工序列(artificialsequence)
<400>52
taaggctagtccgttatcaacttgaaaaagtggcacccttcctcgctcactgactcgc58
<210>53
<211>56
<212>dna
<213>人工序列(artificialsequence)
<400>53
ttaaaggatttgagcgtagcgaaaaatcctttctgtcagaccaagtttactcatat56
<210>54
<211>59
<212>dna
<213>人工序列(artificialsequence)
<400>54
caatctaaagtatatatgagtaaacttggtctgacagaaaggatttttcgctacgctca59
<210>55
<211>43
<212>dna
<213>人工序列(artificialsequence)
<400>55
gtgtgatgcgctgcgtccattaaaggatatggatctggagctg43
<210>56
<211>43
<212>dna
<213>人工序列(artificialsequence)
<400>56
cagctccagatccatatcctttaatggacgcagcgcatcacac43
<210>57
<211>61
<212>dna
<213>人工序列(artificialsequence)
<400>57
attgattcttttttaagtgttacccctataagttagtgtcttttgcgcagtcggcttaaa60
c61
<210>58
<211>47
<212>dna
<213>人工序列(artificialsequence)
<400>58
gtttaagccgactgcgcaaaagacactaacttataggggtaacactt47
<210>59
<211>59
<212>dna
<213>人工序列(artificialsequence)
<400>59
tatctaagcctattgagtatttcttatccatggatcccatttccccctttgatttttag59
<210>60
<211>49
<212>dna
<213>人工序列(artificialsequence)
<400>60
tcaaagggggaaatgggatccatggataagaaatactcaataggcttag49
<210>61
<211>51
<212>dna
<213>人工序列(artificialsequence)
<400>61
atgccccggcctgcatgcacgtcagtcacctcctagctgactcaaatcaat51
<210>62
<211>23
<212>dna
<213>人工序列(artificialsequence)
<400>62
taggcttagctatcggcacaaat23
<210>63
<211>24
<212>dna
<213>人工序列(artificialsequence)
<400>63
ttgagtatttcttatccatggatc24
<210>64
<211>87
<212>dna
<213>人工序列(artificialsequence)
<400>64
cacaccaggtctcagattttaatgagctgatttcggtatacagttgagacaagatctaag60
cttggctgcaggtcgacggatccccgg87
<210>65
<211>89
<212>dna
<213>人工序列(artificialsequence)
<400>65
cacaccaggtctcaaatcgctttttttaccataggttccggtaataaaggcatttttccc60
tggcgtaatcatggtcatagctgtttcct89
<210>66
<211>87
<212>dna
<213>人工序列(artificialsequence)
<400>66
cacaccaggtctcattaaaataaggctagtccgttatcaacttgaaaaagtggcaccaag60
cttggctgcaggtcgacggatccccgg87
<210>67
<211>89
<212>dna
<213>人工序列(artificialsequence)
<400>67
cacaccaggtctcattaacttgctatttctagctctaaaacttacatttcgaatcgtcag60
cagatcttgtctcaactgtataccgaaat89
<210>68
<211>89
<212>dna
<213>人工序列(artificialsequence)
<400>68
cacaccaggtctcattaacttgctatttctagctctaaaacttacatttcgaatcgtcag60
cagatcttgtctcaactgtataccgaaat89
<210>69
<211>56
<212>dna
<213>人工序列(artificialsequence)
<400>69
cacaccaggtctcatcctgcctcgtgatacgcctatttttataggttaatgtcatg56
<210>70
<211>40
<212>dna
<213>人工序列(artificialsequence)
<400>70
cacaccaggtctcaaggaggcttacttgtctgctttcttc40
<210>71
<211>41
<212>dna
<213>人工序列(artificialsequence)
<400>71
cacaccaggtctcataatgtcactaacctgccccgttagtc41
<210>72
<211>41
<212>dna
<213>人工序列(artificialsequence)
<400>72
ggtaagagaggaatgtacacgctgtttcctgtgtgaaattg41
<210>73
<211>74
<212>dna
<213>人工序列(artificialsequence)
<400>73
taacggactagccttattttaacttgctatttctagctctaaaacgacgaaagggcctcg60
tgatacgcctattt74
<210>74
<211>74
<212>dna
<213>人工序列(artificialsequence)
<400>74
atagcaagttaaaataaggctagtccgttatcaacttgaaaaagtggcacccgtgcatgc60
aggccggggcatat74
<210>75
<211>50
<212>dna
<213>人工序列(artificialsequence)
<400>75
caatttcacacaggaaacagcgtgtacattcctctcttacctataatggt50
<210>76
<211>4107
<212>dna
<213>人工序列(artificialsequence)
<400>76
atggataagaaatactcaataggcttagatatcggcacaaatagcgtcggatgggcggtg60
atcactgatgaatataaggttccgtctaaaaagttcaaggttctgggaaatacagaccgc120
cacagtatcaaaaaaaatcttataggggctcttttatttgacagtggagagacagcggaa180
gcgactcgtctcaaacggacagctcgtagaaggtatacacgtcggaagaatcgtatttgt240
tatctacaggagattttttcaaatgagatggcgaaagtagatgatagtttctttcatcga300
cttgaagagtcttttttggtggaagaagacaagaagcatgaacgtcatcctatttttgga360
aatatagtagatgaagttgcttatcatgagaaatatccaactatctatcatctgcgaaaa420
aaattggtagattctactgataaagcggatttgcgcttaatctatttggccttagcgcat480
atgattaagtttcgtggtcattttttgattgagggagatttaaatcctgataatagtgat540
gtggacaaactatttatccagttggtacaaacctacaatcaattatttgaagaaaaccct600
attaacgcaagtggagtagatgctaaagcgattctttctgcacgattgagtaaatcaaga660
cgattagaaaatctcattgctcagctccccggtgagaagaaaaatggcttatttgggaat720
ctcattgctttgtcattgggtttgacccctaattttaaatcaaattttgatttggcagaa780
gatgctaaattacagctttcaaaagatacttacgatgatgatttagataatttattggcg840
caaattggagatcaatatgctgatttgtttttggcagctaagaatttatcagatgctatt900
ttactttcagatatcctaagagtaaatactgaaataactaaggctcccctatcagcttca960
atgattaaacgctacgatgaacatcatcaagacttgactcttttaaaagctttagttcga1020
caacaacttccagaaaagtataaagaaatcttttttgatcaatcaaaaaacggatatgca1080
ggttatattgatgggggagctagccaagaagaattttataaatttatcaaaccaatttta1140
gaaaaaatggatggtactgaggaattattggtgaaactaaatcgtgaagatttgctgcgc1200
aagcaacggacctttgacaacggctctattccccatcaaattcacttgggtgagctgcat1260
gctattttgagaagacaagaagacttttatccatttttaaaagacaatcgtgagaagatt1320
gaaaaaatcttgacttttcgaattccttattatgttggtccattggcgcgtggcaatagt1380
cgttttgcatggatgactcggaagtctgaagaaacaattaccccatggaattttgaagaa1440
gttgtcgataaaggtgcttcagctcaatcatttattgaacgcatgacaaactttgataaa1500
aatcttccaaatgaaaaagtactaccaaaacatagtttgctttatgagtattttacggtt1560
tataacgaattgacaaaggtcaaatatgttactgaaggaatgcgaaaaccagcatttctt1620
tcaggtgaacagaagaaagccattgttgatttactcttcaaaacaaatcgaaaagtaacc1680
gttaagcaattaaaagaagattatttcaaaaaaatagaatgttttgatagtgttgaaatt1740
tcaggagttgaagatagatttaatgcttcattaggtacctaccatgatttgctaaaaatt1800
attaaagataaagattttttggataatgaagaaaatgaagatatcttagaggatattgtt1860
ttaacattgaccttatttgaagatagggagatgattgaggaaagacttaaaacatatgct1920
cacctctttgatgataaggtgatgaaacagcttaaacgtcgccgttatactggttgggga1980
cgtttgtctcgaaaattgattaatggtattagggataagcaatctggcaaaacaatatta2040
gattttttgaaatcagatggttttgccaatcgcaattttatgcagctgatccatgatgat2100
agtttgacatttaaagaagacattcaaaaagcacaagtgtctggacaaggcgatagttta2160
catgaacatattgcaaatttagctggtagccctgctattaaaaaaggtattttacagact2220
gtaaaagttgttgatgaattggtcaaagtaatggggcggcataagccagaaaatatcgtt2280
attgaaatggcacgtgaaaatcagacaactcaaaagggccagaaaaattcgcgagagcgt2340
atgaaacgaatcgaagaaggtatcaaagaattaggaagtcagattcttaaagagcatcct2400
gttgaaaatactcaattgcaaaatgaaaagctctatctctattatctccaaaatggaaga2460
gacatgtatgtggaccaagaattagatattaatcgtttaagtgattatgatgtcgatcac2520
attgttccacaaagtttccttaaagacgattcaatagacaataaggtcttaacgcgttct2580
gataaaaatcgtggtaaatcggataacgttccaagtgaagaagtagtcaaaaagatgaaa2640
aactattggagacaacttctaaacgccaagttaatcactcaacgtaagtttgataattta2700
acgaaagctgaacgtggaggtttgagtgaacttgataaagctggttttatcaaacgccaa2760
ttggttgaaactcgccaaatcactaagcatgtggcacaaattttggatagtcgcatgaat2820
actaaatacgatgaaaatgataaacttattcgagaggttaaagtgattaccttaaaatct2880
aaattagtttctgacttccgaaaagatttccaattctataaagtacgtgagattaacaat2940
taccatcatgcccatgatgcgtatctaaatgccgtcgttggaactgctttgattaagaaa3000
tatccaaaacttgaatcggagtttgtctatggtgattataaagtttatgatgttcgtaaa3060
atgattgctaagtctgagcaagaaataggcaaagcaaccgcaaaatatttcttttactct3120
aatatcatgaacttcttcaaaacagaaattacacttgcaaatggagagattcgcaaacgc3180
cctctaatcgaaactaatggggaaactggagaaattgtctgggataaagggcgagatttt3240
gccacagtgcgcaaagtattgtccatgccccaagtcaatattgtcaagaaaacagaagta3300
cagacaggcggattctccaaggagtcaattttaccaaaaagaaattcggacaagcttatt3360
gctcgtaaaaaagactgggatccaaaaaaatatggtggttttgatagtccaacggtagct3420
tattcagtcctagtggttgctaaggtggaaaaagggaaatcgaagaagttaaaatccgtt3480
aaagagttactagggatcacaattatggaaagaagttcctttgaaaaaaatccgattgac3540
tttttagaagctaaaggatataaggaagttaaaaaagacttaatcattaaactacctaaa3600
tatagtctttttgagttagaaaacggtcgtaaacggatgctggctagtgccggagaatta3660
caaaaaggaaatgagctggctctgccaagcaaatatgtgaattttttatatttagctagt3720
cattatgaaaagttgaagggtagtccagaagataacgaacaaaaacaattgtttgtggag3780
cagcataagcattatttagatgagattattgagcaaatcagtgaattttctaagcgtgtt3840
attttagcagatgccaatttagataaagttcttagtgcatataacaaacatagagacaaa3900
ccaatacgtgaacaagcagaaaatattattcatttatttacgttgacgaatcttggagct3960
cccgctgcttttaaatattttgatacaacaattgatcgtaaacgatatacgtctacaaaa4020
gaagttttagatgccactcttatccatcaatccatcactggtctttatgaaacacgcatt4080
gatttgagtcagctaggaggtgactga4107
<210>77
<211>1008
<212>dna
<213>人工序列(artificialsequence)
<400>77
aaaggatttttcgctacgctcaaatcctttaaaaaaacacaaaagaccacattttttaat60
gtggtctttattcttcaactaaagcacccattagttcaacaaacgaaaattggataaagt120
gggatatttttaaaatatatatttatgttacagtaatattgacttttaaaaaaggattga180
ttctaatgaagaaagcagacaagtaagcctcctaaattcactttagataaaaatttagga240
ggcatatcaaatgaactttaataaaattgatttagacaattggaagagaaaagagatatt300
taatcattatttgaaccaacaaacgacttttagtataaccacagaaattgatattagtgt360
tttataccgaaacataaaacaagaaggatataaattttaccctgcatttattttcttagt420
gacaagggtgataaactcaaatacagcttttagaactggttacaatagcgacggagagtt480
aggttattgggataagttagagccactttatacaatttttgatggtgtatctaaaacatt540
ctctggtatttggactcctgtaaagaatgacttcaaagagttttatgatttatacctttc600
tgatgtagagaaatataatggttcggggaaattgtttcccaaaacacctatacctgaaaa660
tgctttttctctttctattattccatggacttcatttactgggtttaacttaaatatcaa720
taataatagtaattaccttctacccattattacagcaggaaaattcattaataaaggtaa780
ttcaatatatttaccgctatctttacaggtacatcattctgtttgtgatggttatcatgc840
aggattgtttatgaactctattcaggaattgtcagataggcctaatgactggcttttata900
atatgagataatgccgactgtactttttacagtcggttttctaatgtcactaacctgccc960
cgttagttgaagaaggtttttatattacagctccagatccatatcctt1008
<210>78
<211>1186
<212>dna
<213>人工序列(artificialsequence)
<400>78
taatggacgcagcgcatcacacgcaaaaaggaaattggaataaatgcgaaatttgagatg60
ttaattaaagacctttttgaggtctttttttcttagatttttggggttatttaggggaga120
aaacataggggggtactacgacctcccccctaggtgtccattgtccattgtccaaacaaa180
taaataaatattgggtttttaatgttaaaaggttgttttttatgttaaagtgaaaaaaac240
agatgttgggaggtacagtgatggttgtagatagaaaagaagagaaaaaagttgctgtta300
ctttaagacttacaacagaagaaaatgagatattaaatagaatcaaagaaaaatataata360
ttagcaaatcagatgcaaccggtattctaataaaaaaatatgcaaaggaggaatacggtg420
cattttaaacaaaaaaagatagacagcactggcatgctgcctatctatgactaaattttg480
ttaagtgtattagcaccgttattatatgatgagcgaaaatgtaataaaagaaactgaaaa540
caagaaaaattcaagaggacgtaattggacatttgttttatatccagaatcagcaaaagc600
cgagtggttagagtatttaaaagagttacacattcaatttgtagtgtctccattacatga660
tagggatactgatacagaaggtaggatgaaaaaagagcattatcatattctagtgatgta720
tgagggtaataaatcttatgaacagataaaaataattaacagaagaattgaatgcgacta780
ttccgcagattgcaggaagtgtgaaaggtcttgtgagatatatgcttcacatggacgatc840
ctaataaatttaaatatcaaaaagaagatatgatagtttatggcggtgtagatgttgatg900
aattattaaagaaaacaacaacagatagatataaattaattaaagaaatgattgagttta960
ttgatgaacaaggaatcgtagaatttaagagtttaatggattatgcaatgaagtttaaat1020
ttgatgattggttcccgcttttatgtgataactcggcgtatgttattcaagaatatataa1080
aatcaaatcggtataaatctgaccgatagattttgaatttaggtgtcacaagacactctt1140
ttttcgcaccagcgaaaactggtttaagccgactgcgcaaaagaca1186
<210>79
<211>934
<212>dna
<213>人工序列(artificialsequence)
<400>79
cttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctc60
actcaaaggcggtaatacggttatccacagaatcaggggataacgcaggaaagaacatgt120
gagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgtttttcc180
ataggctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcagaggtggcgaa240
acccgacaggactataaagataccaggcgtttccccctggaagctccctcgtgcgctctc300
ctgttccgaccctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtgg360
cgctttctcatagctcacgctgtaggtatctcagttcggtgtaggtcgttcgctccaagc420
tgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccggtaactatc480
gtcttgagtccaacccggtaagacacgacttatcgccactggcagcagccactggtaaca540
ggattagcagagcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaact600
acggctacactagaaggacagtatttggtatctgcgctctgctgaagccagttaccttcg660
gaaaaagagttggtagctcttgatccggcaaacaaaccaccgctggtagcggtggttttt720
ttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatct780
tttctacggggtctgacgctcagtggaacgaaaactcacgttaagggattttggtcatga840
gattatcaaaaaggatcttcacctagatccttttaaattaaaaatgaagttttaaatcaa900
tctaaagtatatatgagtaaacttggtctgacag934
<210>80
<211>440
<212>dna
<213>人工序列(artificialsequence)
<400>80
cgtgcatgcaggccggggcatatgggaaacagcgcggacgcagcggaatttccaatttca60
tgccgcagccgcctgcgctgttctcatttgcggcttccttgtagagctcagcattattga120
gtggatgattatattccttttgataggtggtatgttttcgcttgaacttttaaatacagc180
cattgaacatacggttgatttaataactgacaaacatcaccctcttgctaaagcggccaa240
ggacgctgccgccggggctgtttgcgtttttgccgtgatttcgtgtatcattggtttact300
tatttttttgccaaagctgtaatggctgaaaattcttacatttattttacatttttagaa360
atgggcgtgaaaaaaagcgcgcgattatgtaaaatataaagtgatagcggtaccattata420
ggtaagagaggaatgtacac440
<210>81
<211>1440
<212>dna
<213>人工序列(artificialsequence)
<400>81
ctaacttataggggtaacacttaaaaaagaatcaataacgatagaaaccgctcctaaagc60
aggtgcattttttcctaacgaagaaggcaatagttcacatttattgtctaaatgagaatg120
gactctagaagaaacttcgtttttaatcgtatttaaaacaatgggatgagattcaattat180
atgatttctcaagataacagcttctatatcaaatgtattaaggatattggttaatccaat240
tccgatataaaagccaaagttttgaagtgcatttaacatttctacatcatttttatttgc300
gcgttccacaatctcttttcgagaaatattcttttcttctttagagagcgaagccagtaa360
cgctttttcagaagcatataattcccaacagcctcgatttccacagctgcatttgggtcc420
attaaaatctatcgtcatatgacccatttccccagaaaaaccctgaacacctttatacaa480
ttcgttgttaataacaagtccagttccaattccgatattaatactgatgtaaacgatgtt540
ttcatagttttttgtcataccaaatactttttcaccgtatgctcctgcattagcttcatt600
ttcaacaaaaaccggaacattaaactcactctcaattaaaaactgcaaatctttgatatt660
ccaatttaagttaggcatgaaaataatttgctgatgacgatctacaaggcctggaacaca720
aattcctattccgactagaccataaggggactcaggcatatgggttacaaaaccatgaat780
aagtgcaaataaaatctcttttacttcactagcggaagaactagacaagtcagaagtctt840
ctcgagaataatatttccttctaagtcggttagaattccgttaagatagtcgactcctat900
atcaataccaatcgagtagcctgcattcttattaaaaacaagcattacaggtcttctgcc960
gcctctagattgccctgccccaatttcaaaaataaaatctttttcaagcagtgtatttac1020
ttgagaggagacagtagacttgtttaatcctgtaatctcagagagagttgccctggagac1080
aggggagttcttcaaaatttcatctaatattaatttttgattcattttttttactaaagc1140
ttgatctgcaatttgaataataaccactcctttgtttatccaccgaactaagttggtgtt1200
ttttgaagcttgaattagatatttaaaagtatcatatctaatattataactaaattttct1260
aaaaaaaacattgaaataaacatttattttgtatatgatgagataaagttagtttattgg1320
ataaacaaactaactcaattaagatagttgatggataaacttgttcacttaaatcaaagg1380
gggaaatgacaaatggtccaaactagtgatatctaaaaatcaaagggggaaatgggatcc1440
<210>82
<211>9237
<212>dna
<213>人工序列(artificialsequence)
<400>82
ctaacttataggggtaacacttaaaaaagaatcaataacgatagaaaccgctcctaaagc60
aggtgcattttttcctaacgaagaaggcaatagttcacatttattgtctaaatgagaatg120
gactctagaagaaacttcgtttttaatcgtatttaaaacaatgggatgagattcaattat180
atgatttctcaagataacagcttctatatcaaatgtattaaggatattggttaatccaat240
tccgatataaaagccaaagttttgaagtgcatttaacatttctacatcatttttatttgc300
gcgttccacaatctcttttcgagaaatattcttttcttctttagagagcgaagccagtaa360
cgctttttcagaagcatataattcccaacagcctcgatttccacagctgcatttgggtcc420
attaaaatctatcgtcatatgacccatttccccagaaaaaccctgaacacctttatacaa480
ttcgttgttaataacaagtccagttccaattccgatattaatactgatgtaaacgatgtt540
ttcatagttttttgtcataccaaatactttttcaccgtatgctcctgcattagcttcatt600
ttcaacaaaaaccggaacattaaactcactctcaattaaaaactgcaaatctttgatatt660
ccaatttaagttaggcatgaaaataatttgctgatgacgatctacaaggcctggaacaca720
aattcctattccgactagaccataaggggactcaggcatatgggttacaaaaccatgaat780
aagtgcaaataaaatctcttttacttcactagcggaagaactagacaagtcagaagtctt840
ctcgagaataatatttccttctaagtcggttagaattccgttaagatagtcgactcctat900
atcaataccaatcgagtagcctgcattcttattaaaaacaagcattacaggtcttctgcc960
gcctctagattgccctgccccaatttcaaaaataaaatctttttcaagcagtgtatttac1020
ttgagaggagacagtagacttgtttaatcctgtaatctcagagagagttgccctggagac1080
aggggagttcttcaaaatttcatctaatattaatttttgattcattttttttactaaagc1140
ttgatctgcaatttgaataataaccactcctttgtttatccaccgaactaagttggtgtt1200
ttttgaagcttgaattagatatttaaaagtatcatatctaatattataactaaattttct1260
aaaaaaaacattgaaataaacatttattttgtatatgatgagataaagttagtttattgg1320
ataaacaaactaactcaattaagatagttgatggataaacttgttcacttaaatcaaagg1380
gggaaatgacaaatggtccaaactagtgatatctaaaaatcaaagggggaaatgggatcc1440
atggataagaaatactcaataggcttagatatcggcacaaatagcgtcggatgggcggtg1500
atcactgatgaatataaggttccgtctaaaaagttcaaggttctgggaaatacagaccgc1560
cacagtatcaaaaaaaatcttataggggctcttttatttgacagtggagagacagcggaa1620
gcgactcgtctcaaacggacagctcgtagaaggtatacacgtcggaagaatcgtatttgt1680
tatctacaggagattttttcaaatgagatggcgaaagtagatgatagtttctttcatcga1740
cttgaagagtcttttttggtggaagaagacaagaagcatgaacgtcatcctatttttgga1800
aatatagtagatgaagttgcttatcatgagaaatatccaactatctatcatctgcgaaaa1860
aaattggtagattctactgataaagcggatttgcgcttaatctatttggccttagcgcat1920
atgattaagtttcgtggtcattttttgattgagggagatttaaatcctgataatagtgat1980
gtggacaaactatttatccagttggtacaaacctacaatcaattatttgaagaaaaccct2040
attaacgcaagtggagtagatgctaaagcgattctttctgcacgattgagtaaatcaaga2100
cgattagaaaatctcattgctcagctccccggtgagaagaaaaatggcttatttgggaat2160
ctcattgctttgtcattgggtttgacccctaattttaaatcaaattttgatttggcagaa2220
gatgctaaattacagctttcaaaagatacttacgatgatgatttagataatttattggcg2280
caaattggagatcaatatgctgatttgtttttggcagctaagaatttatcagatgctatt2340
ttactttcagatatcctaagagtaaatactgaaataactaaggctcccctatcagcttca2400
atgattaaacgctacgatgaacatcatcaagacttgactcttttaaaagctttagttcga2460
caacaacttccagaaaagtataaagaaatcttttttgatcaatcaaaaaacggatatgca2520
ggttatattgatgggggagctagccaagaagaattttataaatttatcaaaccaatttta2580
gaaaaaatggatggtactgaggaattattggtgaaactaaatcgtgaagatttgctgcgc2640
aagcaacggacctttgacaacggctctattccccatcaaattcacttgggtgagctgcat2700
gctattttgagaagacaagaagacttttatccatttttaaaagacaatcgtgagaagatt2760
gaaaaaatcttgacttttcgaattccttattatgttggtccattggcgcgtggcaatagt2820
cgttttgcatggatgactcggaagtctgaagaaacaattaccccatggaattttgaagaa2880
gttgtcgataaaggtgcttcagctcaatcatttattgaacgcatgacaaactttgataaa2940
aatcttccaaatgaaaaagtactaccaaaacatagtttgctttatgagtattttacggtt3000
tataacgaattgacaaaggtcaaatatgttactgaaggaatgcgaaaaccagcatttctt3060
tcaggtgaacagaagaaagccattgttgatttactcttcaaaacaaatcgaaaagtaacc3120
gttaagcaattaaaagaagattatttcaaaaaaatagaatgttttgatagtgttgaaatt3180
tcaggagttgaagatagatttaatgcttcattaggtacctaccatgatttgctaaaaatt3240
attaaagataaagattttttggataatgaagaaaatgaagatatcttagaggatattgtt3300
ttaacattgaccttatttgaagatagggagatgattgaggaaagacttaaaacatatgct3360
cacctctttgatgataaggtgatgaaacagcttaaacgtcgccgttatactggttgggga3420
cgtttgtctcgaaaattgattaatggtattagggataagcaatctggcaaaacaatatta3480
gattttttgaaatcagatggttttgccaatcgcaattttatgcagctgatccatgatgat3540
agtttgacatttaaagaagacattcaaaaagcacaagtgtctggacaaggcgatagttta3600
catgaacatattgcaaatttagctggtagccctgctattaaaaaaggtattttacagact3660
gtaaaagttgttgatgaattggtcaaagtaatggggcggcataagccagaaaatatcgtt3720
attgaaatggcacgtgaaaatcagacaactcaaaagggccagaaaaattcgcgagagcgt3780
atgaaacgaatcgaagaaggtatcaaagaattaggaagtcagattcttaaagagcatcct3840
gttgaaaatactcaattgcaaaatgaaaagctctatctctattatctccaaaatggaaga3900
gacatgtatgtggaccaagaattagatattaatcgtttaagtgattatgatgtcgatcac3960
attgttccacaaagtttccttaaagacgattcaatagacaataaggtcttaacgcgttct4020
gataaaaatcgtggtaaatcggataacgttccaagtgaagaagtagtcaaaaagatgaaa4080
aactattggagacaacttctaaacgccaagttaatcactcaacgtaagtttgataattta4140
acgaaagctgaacgtggaggtttgagtgaacttgataaagctggttttatcaaacgccaa4200
ttggttgaaactcgccaaatcactaagcatgtggcacaaattttggatagtcgcatgaat4260
actaaatacgatgaaaatgataaacttattcgagaggttaaagtgattaccttaaaatct4320
aaattagtttctgacttccgaaaagatttccaattctataaagtacgtgagattaacaat4380
taccatcatgcccatgatgcgtatctaaatgccgtcgttggaactgctttgattaagaaa4440
tatccaaaacttgaatcggagtttgtctatggtgattataaagtttatgatgttcgtaaa4500
atgattgctaagtctgagcaagaaataggcaaagcaaccgcaaaatatttcttttactct4560
aatatcatgaacttcttcaaaacagaaattacacttgcaaatggagagattcgcaaacgc4620
cctctaatcgaaactaatggggaaactggagaaattgtctgggataaagggcgagatttt4680
gccacagtgcgcaaagtattgtccatgccccaagtcaatattgtcaagaaaacagaagta4740
cagacaggcggattctccaaggagtcaattttaccaaaaagaaattcggacaagcttatt4800
gctcgtaaaaaagactgggatccaaaaaaatatggtggttttgatagtccaacggtagct4860
tattcagtcctagtggttgctaaggtggaaaaagggaaatcgaagaagttaaaatccgtt4920
aaagagttactagggatcacaattatggaaagaagttcctttgaaaaaaatccgattgac4980
tttttagaagctaaaggatataaggaagttaaaaaagacttaatcattaaactacctaaa5040
tatagtctttttgagttagaaaacggtcgtaaacggatgctggctagtgccggagaatta5100
caaaaaggaaatgagctggctctgccaagcaaatatgtgaattttttatatttagctagt5160
cattatgaaaagttgaagggtagtccagaagataacgaacaaaaacaattgtttgtggag5220
cagcataagcattatttagatgagattattgagcaaatcagtgaattttctaagcgtgtt5280
attttagcagatgccaatttagataaagttcttagtgcatataacaaacatagagacaaa5340
ccaatacgtgaacaagcagaaaatattattcatttatttacgttgacgaatcttggagct5400
cccgctgcttttaaatattttgatacaacaattgatcgtaaacgatatacgtctacaaaa5460
gaagttttagatgccactcttatccatcaatccatcactggtctttatgaaacacgcatt5520
gatttgagtcagctaggaggtgactgacgtgcatgcaggccggggcatatgggaaacagc5580
gcggacgcagcggaatttccaatttcatgccgcagccgcctgcgctgttctcatttgcgg5640
cttccttgtagagctcagcattattgagtggatgattatattccttttgataggtggtat5700
gttttcgcttgaacttttaaatacagccattgaacatacggttgatttaataactgacaa5760
acatcaccctcttgctaaagcggccaaggacgctgccgccggggctgtttgcgtttttgc5820
cgtgatttcgtgtatcattggtttacttatttttttgccaaagctgtaatggctgaaaat5880
tcttacatttattttacatttttagaaatgggcgtgaaaaaaagcgcgcgattatgtaaa5940
atataaagtgatagcggtaccattataggtaagagaggaatgtacacgtttcggtgatga6000
cctttccagtcgggaaacctgtcgtaggtcgttcgctccaagcgttttagagctagaaat6060
agcaagttaaaataaggctagtccgttatcaacttgaaaaagtggcacccttcctcgctc6120
actgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcg6180
gtaatacggttatccacagaatcaggggataacgcaggaaagaacatgtgagcaaaaggc6240
cagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgtttttccataggctccgc6300
ccccctgacgagcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacagga6360
ctataaagataccaggcgtttccccctggaagctccctcgtgcgctctcctgttccgacc6420
ctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcat6480
agctcacgctgtaggtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgtg6540
cacgaaccccccgttcagcccgaccgctgcgccttatccggtaactatcgtcttgagtcc6600
aacccggtaagacacgacttatcgccactggcagcagccactggtaacaggattagcaga6660
gcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacggctacact6720
agaaggacagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagtt6780
ggtagctcttgatccggcaaacaaaccaccgctggtagcggtggtttttttgtttgcaag6840
cagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttctacgggg6900
tctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaa6960
aggatcttcacctagatccttttaaattaaaaatgaagttttaaatcaatctaaagtata7020
tatgagtaaacttggtctgacagaaaggatttttcgctacgctcaaatcctttaaaaaaa7080
cacaaaagaccacattttttaatgtggtctttattcttcaactaaagcacccattagttc7140
aacaaacgaaaattggataaagtgggatatttttaaaatatatatttatgttacagtaat7200
attgacttttaaaaaaggattgattctaatgaagaaagcagacaagtaagcctcctaaat7260
tcactttagataaaaatttaggaggcatatcaaatgaactttaataaaattgatttagac7320
aattggaagagaaaagagatatttaatcattatttgaaccaacaaacgacttttagtata7380
accacagaaattgatattagtgttttataccgaaacataaaacaagaaggatataaattt7440
taccctgcatttattttcttagtgacaagggtgataaactcaaatacagcttttagaact7500
ggttacaatagcgacggagagttaggttattgggataagttagagccactttatacaatt7560
tttgatggtgtatctaaaacattctctggtatttggactcctgtaaagaatgacttcaaa7620
gagttttatgatttatacctttctgatgtagagaaatataatggttcggggaaattgttt7680
cccaaaacacctatacctgaaaatgctttttctctttctattattccatggacttcattt7740
actgggtttaacttaaatatcaataataatagtaattaccttctacccattattacagca7800
ggaaaattcattaataaaggtaattcaatatatttaccgctatctttacaggtacatcat7860
tctgtttgtgatggttatcatgcaggattgtttatgaactctattcaggaattgtcagat7920
aggcctaatgactggcttttataatatgagataatgccgactgtactttttacagtcggt7980
tttctaatgtcactaacctgccccgttagttgaagaaggtttttatattacagctccaga8040
tccatatcctttaatggacgcagcgcatcacacgcaaaaaggaaattggaataaatgcga8100
aatttgagatgttaattaaagacctttttgaggtctttttttcttagatttttggggtta8160
tttaggggagaaaacataggggggtactacgacctcccccctaggtgtccattgtccatt8220
gtccaaacaaataaataaatattgggtttttaatgttaaaaggttgttttttatgttaaa8280
gtgaaaaaaacagatgttgggaggtacagtgatggttgtagatagaaaagaagagaaaaa8340
agttgctgttactttaagacttacaacagaagaaaatgagatattaaatagaatcaaaga8400
aaaatataatattagcaaatcagatgcaaccggtattctaataaaaaaatatgcaaagga8460
ggaatacggtgcattttaaacaaaaaaagatagacagcactggcatgctgcctatctatg8520
actaaattttgttaagtgtattagcaccgttattatatgatgagcgaaaatgtaataaaa8580
gaaactgaaaacaagaaaaattcaagaggacgtaattggacatttgttttatatccagaa8640
tcagcaaaagccgagtggttagagtatttaaaagagttacacattcaatttgtagtgtct8700
ccattacatgatagggatactgatacagaaggtaggatgaaaaaagagcattatcatatt8760
ctagtgatgtatgagggtaataaatcttatgaacagataaaaataattaacagaagaatt8820
gaatgcgactattccgcagattgcaggaagtgtgaaaggtcttgtgagatatatgcttca8880
catggacgatcctaataaatttaaatatcaaaaagaagatatgatagtttatggcggtgt8940
agatgttgatgaattattaaagaaaacaacaacagatagatataaattaattaaagaaat9000
gattgagtttattgatgaacaaggaatcgtagaatttaagagtttaatggattatgcaat9060
gaagtttaaatttgatgattggttcccgcttttatgtgataactcggcgtatgttattca9120
agaatatataaaatcaaatcggtataaatctgaccgatagattttgaatttaggtgtcac9180
aagacactcttttttcgcaccagcgaaaactggtttaagccgactgcgcaaaagaca9237
<210>83
<211>9237
<212>dna
<213>人工序列(artificialsequence)
<400>83
ctaacttataggggtaacacttaaaaaagaatcaataacgatagaaaccgctcctaaagc60
aggtgcattttttcctaacgaagaaggcaatagttcacatttattgtctaaatgagaatg120
gactctagaagaaacttcgtttttaatcgtatttaaaacaatgggatgagattcaattat180
atgatttctcaagataacagcttctatatcaaatgtattaaggatattggttaatccaat240
tccgatataaaagccaaagttttgaagtgcatttaacatttctacatcatttttatttgc300
gcgttccacaatctcttttcgagaaatattcttttcttctttagagagcgaagccagtaa360
cgctttttcagaagcatataattcccaacagcctcgatttccacagctgcatttgggtcc420
attaaaatctatcgtcatatgacccatttccccagaaaaaccctgaacacctttatacaa480
ttcgttgttaataacaagtccagttccaattccgatattaatactgatgtaaacgatgtt540
ttcatagttttttgtcataccaaatactttttcaccgtatgctcctgcattagcttcatt600
ttcaacaaaaaccggaacattaaactcactctcaattaaaaactgcaaatctttgatatt660
ccaatttaagttaggcatgaaaataatttgctgatgacgatctacaaggcctggaacaca720
aattcctattccgactagaccataaggggactcaggcatatgggttacaaaaccatgaat780
aagtgcaaataaaatctcttttacttcactagcggaagaactagacaagtcagaagtctt840
ctcgagaataatatttccttctaagtcggttagaattccgttaagatagtcgactcctat900
atcaataccaatcgagtagcctgcattcttattaaaaacaagcattacaggtcttctgcc960
gcctctagattgccctgccccaatttcaaaaataaaatctttttcaagcagtgtatttac1020
ttgagaggagacagtagacttgtttaatcctgtaatctcagagagagttgccctggagac1080
aggggagttcttcaaaatttcatctaatattaatttttgattcattttttttactaaagc1140
ttgatctgcaatttgaataataaccactcctttgtttatccaccgaactaagttggtgtt1200
ttttgaagcttgaattagatatttaaaagtatcatatctaatattataactaaattttct1260
aaaaaaaacattgaaataaacatttattttgtatatgatgagataaagttagtttattgg1320
ataaacaaactaactcaattaagatagttgatggataaacttgttcacttaaatcaaagg1380
gggaaatgacaaatggtccaaactagtgatatctaaaaatcaaagggggaaatgggatcc1440
atggataagaaatactcaataggcttagctatcggcacaaatagcgtcggatgggcggtg1500
atcactgatgaatataaggttccgtctaaaaagttcaaggttctgggaaatacagaccgc1560
cacagtatcaaaaaaaatcttataggggctcttttatttgacagtggagagacagcggaa1620
gcgactcgtctcaaacggacagctcgtagaaggtatacacgtcggaagaatcgtatttgt1680
tatctacaggagattttttcaaatgagatggcgaaagtagatgatagtttctttcatcga1740
cttgaagagtcttttttggtggaagaagacaagaagcatgaacgtcatcctatttttgga1800
aatatagtagatgaagttgcttatcatgagaaatatccaactatctatcatctgcgaaaa1860
aaattggtagattctactgataaagcggatttgcgcttaatctatttggccttagcgcat1920
atgattaagtttcgtggtcattttttgattgagggagatttaaatcctgataatagtgat1980
gtggacaaactatttatccagttggtacaaacctacaatcaattatttgaagaaaaccct2040
attaacgcaagtggagtagatgctaaagcgattctttctgcacgattgagtaaatcaaga2100
cgattagaaaatctcattgctcagctccccggtgagaagaaaaatggcttatttgggaat2160
ctcattgctttgtcattgggtttgacccctaattttaaatcaaattttgatttggcagaa2220
gatgctaaattacagctttcaaaagatacttacgatgatgatttagataatttattggcg2280
caaattggagatcaatatgctgatttgtttttggcagctaagaatttatcagatgctatt2340
ttactttcagatatcctaagagtaaatactgaaataactaaggctcccctatcagcttca2400
atgattaaacgctacgatgaacatcatcaagacttgactcttttaaaagctttagttcga2460
caacaacttccagaaaagtataaagaaatcttttttgatcaatcaaaaaacggatatgca2520
ggttatattgatgggggagctagccaagaagaattttataaatttatcaaaccaatttta2580
gaaaaaatggatggtactgaggaattattggtgaaactaaatcgtgaagatttgctgcgc2640
aagcaacggacctttgacaacggctctattccccatcaaattcacttgggtgagctgcat2700
gctattttgagaagacaagaagacttttatccatttttaaaagacaatcgtgagaagatt2760
gaaaaaatcttgacttttcgaattccttattatgttggtccattggcgcgtggcaatagt2820
cgttttgcatggatgactcggaagtctgaagaaacaattaccccatggaattttgaagaa2880
gttgtcgataaaggtgcttcagctcaatcatttattgaacgcatgacaaactttgataaa2940
aatcttccaaatgaaaaagtactaccaaaacatagtttgctttatgagtattttacggtt3000
tataacgaattgacaaaggtcaaatatgttactgaaggaatgcgaaaaccagcatttctt3060
tcaggtgaacagaagaaagccattgttgatttactcttcaaaacaaatcgaaaagtaacc3120
gttaagcaattaaaagaagattatttcaaaaaaatagaatgttttgatagtgttgaaatt3180
tcaggagttgaagatagatttaatgcttcattaggtacctaccatgatttgctaaaaatt3240
attaaagataaagattttttggataatgaagaaaatgaagatatcttagaggatattgtt3300
ttaacattgaccttatttgaagatagggagatgattgaggaaagacttaaaacatatgct3360
cacctctttgatgataaggtgatgaaacagcttaaacgtcgccgttatactggttgggga3420
cgtttgtctcgaaaattgattaatggtattagggataagcaatctggcaaaacaatatta3480
gattttttgaaatcagatggttttgccaatcgcaattttatgcagctgatccatgatgat3540
agtttgacatttaaagaagacattcaaaaagcacaagtgtctggacaaggcgatagttta3600
catgaacatattgcaaatttagctggtagccctgctattaaaaaaggtattttacagact3660
gtaaaagttgttgatgaattggtcaaagtaatggggcggcataagccagaaaatatcgtt3720
attgaaatggcacgtgaaaatcagacaactcaaaagggccagaaaaattcgcgagagcgt3780
atgaaacgaatcgaagaaggtatcaaagaattaggaagtcagattcttaaagagcatcct3840
gttgaaaatactcaattgcaaaatgaaaagctctatctctattatctccaaaatggaaga3900
gacatgtatgtggaccaagaattagatattaatcgtttaagtgattatgatgtcgatcac3960
attgttccacaaagtttccttaaagacgattcaatagacaataaggtcttaacgcgttct4020
gataaaaatcgtggtaaatcggataacgttccaagtgaagaagtagtcaaaaagatgaaa4080
aactattggagacaacttctaaacgccaagttaatcactcaacgtaagtttgataattta4140
acgaaagctgaacgtggaggtttgagtgaacttgataaagctggttttatcaaacgccaa4200
ttggttgaaactcgccaaatcactaagcatgtggcacaaattttggatagtcgcatgaat4260
actaaatacgatgaaaatgataaacttattcgagaggttaaagtgattaccttaaaatct4320
aaattagtttctgacttccgaaaagatttccaattctataaagtacgtgagattaacaat4380
taccatcatgcccatgatgcgtatctaaatgccgtcgttggaactgctttgattaagaaa4440
tatccaaaacttgaatcggagtttgtctatggtgattataaagtttatgatgttcgtaaa4500
atgattgctaagtctgagcaagaaataggcaaagcaaccgcaaaatatttcttttactct4560
aatatcatgaacttcttcaaaacagaaattacacttgcaaatggagagattcgcaaacgc4620
cctctaatcgaaactaatggggaaactggagaaattgtctgggataaagggcgagatttt4680
gccacagtgcgcaaagtattgtccatgccccaagtcaatattgtcaagaaaacagaagta4740
cagacaggcggattctccaaggagtcaattttaccaaaaagaaattcggacaagcttatt4800
gctcgtaaaaaagactgggatccaaaaaaatatggtggttttgatagtccaacggtagct4860
tattcagtcctagtggttgctaaggtggaaaaagggaaatcgaagaagttaaaatccgtt4920
aaagagttactagggatcacaattatggaaagaagttcctttgaaaaaaatccgattgac4980
tttttagaagctaaaggatataaggaagttaaaaaagacttaatcattaaactacctaaa5040
tatagtctttttgagttagaaaacggtcgtaaacggatgctggctagtgccggagaatta5100
caaaaaggaaatgagctggctctgccaagcaaatatgtgaattttttatatttagctagt5160
cattatgaaaagttgaagggtagtccagaagataacgaacaaaaacaattgtttgtggag5220
cagcataagcattatttagatgagattattgagcaaatcagtgaattttctaagcgtgtt5280
attttagcagatgccaatttagataaagttcttagtgcatataacaaacatagagacaaa5340
ccaatacgtgaacaagcagaaaatattattcatttatttacgttgacgaatcttggagct5400
cccgctgcttttaaatattttgatacaacaattgatcgtaaacgatatacgtctacaaaa5460
gaagttttagatgccactcttatccatcaatccatcactggtctttatgaaacacgcatt5520
gatttgagtcagctaggaggtgactgacgtgcatgcaggccggggcatatgggaaacagc5580
gcggacgcagcggaatttccaatttcatgccgcagccgcctgcgctgttctcatttgcgg5640
cttccttgtagagctcagcattattgagtggatgattatattccttttgataggtggtat5700
gttttcgcttgaacttttaaatacagccattgaacatacggttgatttaataactgacaa5760
acatcaccctcttgctaaagcggccaaggacgctgccgccggggctgtttgcgtttttgc5820
cgtgatttcgtgtatcattggtttacttatttttttgccaaagctgtaatggctgaaaat5880
tcttacatttattttacatttttagaaatgggcgtgaaaaaaagcgcgcgattatgtaaa5940
atataaagtgatagcggtaccattataggtaagagaggaatgtacacgtttcggtgatga6000
cctttccagtcgggaaacctgtcgtaggtcgttcgctccaagcgttttagagctagaaat6060
agcaagttaaaataaggctagtccgttatcaacttgaaaaagtggcacccttcctcgctc6120
actgactcgctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcg6180
gtaatacggttatccacagaatcaggggataacgcaggaaagaacatgtgagcaaaaggc6240
cagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcgtttttccataggctccgc6300
ccccctgacgagcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacagga6360
ctataaagataccaggcgtttccccctggaagctccctcgtgcgctctcctgttccgacc6420
ctgccgcttaccggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcat6480
agctcacgctgtaggtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgtg6540
cacgaaccccccgttcagcccgaccgctgcgccttatccggtaactatcgtcttgagtcc6600
aacccggtaagacacgacttatcgccactggcagcagccactggtaacaggattagcaga6660
gcgaggtatgtaggcggtgctacagagttcttgaagtggtggcctaactacggctacact6720
agaaggacagtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagtt6780
ggtagctcttgatccggcaaacaaaccaccgctggtagcggtggtttttttgtttgcaag6840
cagcagattacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttctacgggg6900
tctgacgctcagtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaa6960
aggatcttcacctagatccttttaaattaaaaatgaagttttaaatcaatctaaagtata7020
tatgagtaaacttggtctgacagaaaggatttttcgctacgctcaaatcctttaaaaaaa7080
cacaaaagaccacattttttaatgtggtctttattcttcaactaaagcacccattagttc7140
aacaaacgaaaattggataaagtgggatatttttaaaatatatatttatgttacagtaat7200
attgacttttaaaaaaggattgattctaatgaagaaagcagacaagtaagcctcctaaat7260
tcactttagataaaaatttaggaggcatatcaaatgaactttaataaaattgatttagac7320
aattggaagagaaaagagatatttaatcattatttgaaccaacaaacgacttttagtata7380
accacagaaattgatattagtgttttataccgaaacataaaacaagaaggatataaattt7440
taccctgcatttattttcttagtgacaagggtgataaactcaaatacagcttttagaact7500
ggttacaatagcgacggagagttaggttattgggataagttagagccactttatacaatt7560
tttgatggtgtatctaaaacattctctggtatttggactcctgtaaagaatgacttcaaa7620
gagttttatgatttatacctttctgatgtagagaaatataatggttcggggaaattgttt7680
cccaaaacacctatacctgaaaatgctttttctctttctattattccatggacttcattt7740
actgggtttaacttaaatatcaataataatagtaattaccttctacccattattacagca7800
ggaaaattcattaataaaggtaattcaatatatttaccgctatctttacaggtacatcat7860
tctgtttgtgatggttatcatgcaggattgtttatgaactctattcaggaattgtcagat7920
aggcctaatgactggcttttataatatgagataatgccgactgtactttttacagtcggt7980
tttctaatgtcactaacctgccccgttagttgaagaaggtttttatattacagctccaga8040
tccatatcctttaatggacgcagcgcatcacacgcaaaaaggaaattggaataaatgcga8100
aatttgagatgttaattaaagacctttttgaggtctttttttcttagatttttggggtta8160
tttaggggagaaaacataggggggtactacgacctcccccctaggtgtccattgtccatt8220
gtccaaacaaataaataaatattgggtttttaatgttaaaaggttgttttttatgttaaa8280
gtgaaaaaaacagatgttgggaggtacagtgatggttgtagatagaaaagaagagaaaaa8340
agttgctgttactttaagacttacaacagaagaaaatgagatattaaatagaatcaaaga8400
aaaatataatattagcaaatcagatgcaaccggtattctaataaaaaaatatgcaaagga8460
ggaatacggtgcattttaaacaaaaaaagatagacagcactggcatgctgcctatctatg8520
actaaattttgttaagtgtattagcaccgttattatatgatgagcgaaaatgtaataaaa8580
gaaactgaaaacaagaaaaattcaagaggacgtaattggacatttgttttatatccagaa8640
tcagcaaaagccgagtggttagagtatttaaaagagttacacattcaatttgtagtgtct8700
ccattacatgatagggatactgatacagaaggtaggatgaaaaaagagcattatcatatt8760
ctagtgatgtatgagggtaataaatcttatgaacagataaaaataattaacagaagaatt8820
gaatgcgactattccgcagattgcaggaagtgtgaaaggtcttgtgagatatatgcttca8880
catggacgatcctaataaatttaaatatcaaaaagaagatatgatagtttatggcggtgt8940
agatgttgatgaattattaaagaaaacaacaacagatagatataaattaattaaagaaat9000
gattgagtttattgatgaacaaggaatcgtagaatttaagagtttaatggattatgcaat9060
gaagtttaaatttgatgattggttcccgcttttatgtgataactcggcgtatgttattca9120
agaatatataaaatcaaatcggtataaatctgaccgatagattttgaatttaggtgtcac9180
aagacactcttttttcgcaccagcgaaaactggtttaagccgactgcgcaaaagaca9237
<210>84
<211>86
<212>dna
<213>人工序列(artificialsequence)
<400>84
agggaaaaatgcctttattaccggaacctatggtaaaaaaagcgattttaatgagctgat60
ttcggtatacagttgagacaagatct86
<210>85
<211>86
<212>dna
<213>人工序列(artificialsequence)
<400>85
gctgacgattcgaaatgtaagttttagagctagaaatagcaagttaaaataaggctagtc60
cgttatcaacttgaaaaagtggcacc86
<210>86
<211>1049
<212>dna
<213>人工序列(artificialsequence)
<400>86
gcctcgtgatacgcctatttttataggttaatgtcatgataataatggtttcttagacgt60
caggtggcacttttcggggaaatgtgcgcggaacccctatttgtttatttttctaaatac120
attcaaatatgtatccgctcatgagacaataaccctgataaatgcttcaataatattgaa180
aaaggaagagtatgagtattcaacatttccgtgtcgcccttattcccttttttgcggcat240
tttgccttcctgtttttgctcacccagaaacgctggtgaaagtaaaagatgctgaagatc300
agttgggtgcacgagtgggttacatcgaactggatctcaacagcggtaagatccttgaga360
gttttcgccccgaagaacgttttccaatgatgagcacttttaaagttctgctatgtggcg420
cggtattatcccgtattgacgccgggcaagagcaactcggtcgccgcatacactattctc480
agaatgacttggttgagtactcaccagtcacagaaaagcatcttacggatggcatgacag540
taagagaattatgcagtgctgccataaccatgagtgataacactgcggccaacttacttc600
tgacaacgatcggaggaccgaaggagctaaccgcttttttgcacaacatgggggatcatg660
taactcgccttgatcgttgggaaccggagctgaatgaagccataccaaacgacgagcgtg720
acaccacgatgcctgtagcaatggcaacaacgttgcgcaaactattaactggcgaactac780
ttactctagcttcccggcaacaattaatagactggatggaggcggataaagttgcaggac840
cacttctgcgctcggcccttccggctggctggtttattgctgataaatctggagccggtg900
agcgtgggtcacgcggtatcattgcagcactggggccagatggtaagccctcccgtatcg960
tagttatctacacgacggggagtcaggcaactatggatgaacgaaatagacagatcgctg1020
agataggtgcctcactgattaagcattgg1049
<210>87
<211>5270
<212>dna
<213>人工序列(artificialsequence)
<400>87
ggttgataatgaactgtgctgattacaaaaatactaaaaatgcccatattttttcctcct60
tataaaattagtataattatagcacgaaaaggatctaggtgaagatcctttttgataatc120
tcatgaccaaaatcccttaacgtgagttttcgttccactgagcgtcagaccccgtagaaa180
agatcaaaggatcttcttgagatcctttttttctgcgcgtaatctgctgcttgcaaacaa240
aaaaaccaccgctaccagcggtggtttgtttgccggatcaagagctaccaactctttttc300
cgaaggtaactggcttcagcagagcgcagataccaaatactgttcttctagtgtagccgt360
agttaggccaccacttcaagaactctgtagcaccgcctacatacctcgctctgctaatcc420
tgttaccagtggctgctgccagtggcgataagtcgtgtcttaccgggttggactcaagac480
gatagttaccggataaggcgcagcggtcgggctgaacggggggttcgtgcacacagccca540
gcttggagcgaacgacctacaccgaactgagatacctacagcgtgagctatgagaaagcg600
ccacgcttcccgaagggagaaaggcggacaggtatccggtaagcggcagggtcggaacag660
gagagcgcacgagggagcttccagggggaaacgcctggtatctttatagtcctgtcgggt720
ttcgccacctctgacttgagcgtcgatttttgtgatgctcgtcaggggggcggagcctat780
ggaaaaacgccagcaacgcggcctttttacggttcctggccttttgctggccttttgctc840
acatgttctttcctgcgttatcccctgattctgtggataaccgtattaccgcctttgagt900
gagctgataccgctcgccgcagccgaacgaccgagcgcagcgagtcagtgagcgaggaag960
cggaagagcgcccaatacgcaaaccgcctctccccgcgcgttggccgattcattaatgca1020
gctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaacgcaattaatgtga1080
gttagctcactcattaggcaccccaggctttacactttatgcttccggctcgtatgttgt1140
gtggaattgtgagcggataacaatttcacacaggaaacagctatgaccatgattacgcca1200
gggaaaaatgcctttattaccggaacctatggtaaaaaaagcgattttaatgagctgatt1260
tcggtatacagttgagacaagatctgctgacgattcgaaatgtaagttttagagctagaa1320
atagcaagttaaaataaggctagtccgttatcaacttgaaaaagtggcaccaagcttggc1380
tgcaggtcgacggatccccgggaattcactggccgtcgttttacaacgtcgtgactggga1440
aaaccctggcgttacccaacttaatcgccttgcagcacatccccctttcgccagctggcg1500
taatagcgaagaggcccgcaccgatcgcccttcccaacagttgcgcagcctgaatggcga1560
atggcgactaacggggcaggttagtgacattagcctcgtgatacgcctatttttataggt1620
taatgtcatgataataatggtttcttagacgtcaggtggcacttttcggggaaatgtgcg1680
cggaacccctatttgtttatttttctaaatacattcaaatatgtatccgctcatgagaca1740
ataaccctgataaatgcttcaataatattgaaaaaggaagagtatgagtattcaacattt1800
ccgtgtcgcccttattcccttttttgcggcattttgccttcctgtttttgctcacccaga1860
aacgctggtgaaagtaaaagatgctgaagatcagttgggtgcacgagtgggttacatcga1920
actggatctcaacagcggtaagatccttgagagttttcgccccgaagaacgttttccaat1980
gatgagcacttttaaagttctgctatgtggcgcggtattatcccgtattgacgccgggca2040
agagcaactcggtcgccgcatacactattctcagaatgacttggttgagtactcaccagt2100
cacagaaaagcatcttacggatggcatgacagtaagagaattatgcagtgctgccataac2160
catgagtgataacactgcggccaacttacttctgacaacgatcggaggaccgaaggagct2220
aaccgcttttttgcacaacatgggggatcatgtaactcgccttgatcgttgggaaccgga2280
gctgaatgaagccataccaaacgacgagcgtgacaccacgatgcctgtagcaatggcaac2340
aacgttgcgcaaactattaactggcgaactacttactctagcttcccggcaacaattaat2400
agactggatggaggcggataaagttgcaggaccacttctgcgctcggcccttccggctgg2460
ctggtttattgctgataaatctggagccggtgagcgtgggtcacgcggtatcattgcagc2520
actggggccagatggtaagccctcccgtatcgtagttatctacacgacggggagtcaggc2580
aactatggatgaacgaaatagacagatcgctgagataggtgcctcactgattaagcattg2640
gaggaggcttacttgtctgctttcttcattagaatcaatccttttttaaaagtcaatatt2700
actgtaacataaatatatattttaaaaatatcccactttatccaattttcgtttgttgaa2760
ctaatgggtgctttagttgaagaataaaagaccacattaaaaaatgtggtcttttgtgtt2820
tttttaaaggatttgagcgtagcgaaaaatccttttctttcttatcttgatactatatag2880
aaacaacatcatttttcaaaattaggtcaaagccttgtgtatcaagggtttgatggttct2940
ttgacaggtaaaaactccttctgctattattaaggtgtcgaatcaaaataatagaatgct3000
agagaactagctcagaaggagtttttttgttgatttattcatctgaaaatgattatagca3060
tcctcgaagataaaaccgcaacaggtaaaaagcgggattggaaggggaaaaagagacgga3120
cgaacctcatggcggagcattacgaagcgttagagagtaagattggggcaccttactatg3180
gcaaaaaggctgaaaaactaattagttgtgcagagtatctttcgtttaagagggatccgg3240
agacgggcaagttaaaactgtatcaagcccatttttgtaaagtgaggttatgtccgatgt3300
gtgcgtggcgcaggtcgttaaaaattgcttatcacaataagttgatcgtagaggaagcca3360
atagacagtacggctgcggatggatttttctcacgctgacgattcgaaatgtaaagggag3420
aacggctgaagccacaaatttctgcgatgatggaaggctttaggaaactgttccagtaca3480
aaaaagtaaaaacttcggttcttggatttttcagagctttagagattaccaaaaatcatg3540
aagaagatacatatcatcctcattttcatgtgttgataccagtaaggaaaaattattttg3600
ggaaaaactatattaagcaggcggagtggacgagcctttggaaaaaggcgatgaaattgg3660
attacactccaattgtcgatattcgtcgagtgaaaggtaaagctaagattgacgctgaac3720
agattgaaaacgatgtgcggaacgcaatgatggagcaaaaagctgttctcgaaatctcta3780
aatatccggttaaggatacggatgttgtgcgcggtaataaggtgactgaagacaatctga3840
acacggtgctttacttggatgatgcgttggcagctcgaaggttaattggatacggtggca3900
ttttgaaggagatacataaagagctgaatcttggtgatgcggaggacggcgatctggtca3960
agattgaggaagaagatgacgaggttgcaaatggtgcatttgaggttatggcttattggc4020
atcctggcattaaaaattacataatcaaataaaaaaagcagacctttagaaggcctgctt4080
ttttaactaacccatttgtattgtgttgaaatatgttttgtatggtgcactctcagtaca4140
atctgctctgatgccgcatagttaagccagccccgacacccgccaacacccgctgacgcg4200
ccctgacgggcttgtctgctcccggcatccgcttacagacaagctgtgaccgtctccggg4260
agctgcatgtgtcagaggttttcaccgtcatcaccgaaacgcgcgagacgaaagggcctc4320
gtgatacgcctatttttataggttaatgtcatgataataatggtttcttagcgattcaca4380
aaaaataggcacacgaaaaacaagttaagggatgcagtttatgcatcccttacttattaa4440
ataatttatagctattgaaaagagataagaattgttcaaagctaatattgtttaaatcgt4500
caattcctgcatgttttaaggaattgttaaattgattttttgtaaatattttcttgtatt4560
ctttgttaacccatttcataacgaaataattatacttttgtttatctttgtgtgatattc4620
ttgatttttttctacttaatctgataagtgagctattcactttaggtttaggatgaaaat4680
attctcttggaaccatacttaatatagaaatatcaacttctgccattaaaagtaatgcca4740
atgagcgttttgtatttaataatcttttagcaaacccgtattccacgattaaataaatct4800
cattagctatactatcaaaaacaattttgcgtattatatccgtacttatgttataaggta4860
tattaccatatattttataggattggtttttaggaaatttaaactgcaatatatccttgt4920
ttaaaacttggaaattatcgtgatcaacaagtttattttctgtagttttgcataatttat4980
ggtctatttcaatggcagttacgaaattacacctctttactaattcaagggtaaaatggc5040
cttttcctgagccgatttcaaagatattatcatgttcatttaatcttatatttgtcatta5100
ttttatctatattatgttttgaagtaataaagttttgactgtgttttatatttttctcgt5160
tcattataaccctctttaatttggttatatgaattttgcttattaacgattcattataac5220
cacttattttttgtttggttgataatgaactgtgctgattacaaaaatac5270
<210>88
<211>2216
<212>dna
<213>人工序列(artificialsequence)
<400>88
gctgtttcctgtgtgaaattgttatccgctcacaattccacacaacatacgagccggaag60
cataaagtgtaaagcctggggtgcctaatgagtgagctaactcacattaattgcgttgcg120
ctcactgcccgctttccagtcgggaaacctgtcgtgccagctgcattaatgaatcggcca180
acgcgcggggagaggcggtttgcgtattgggcgctcttccgcttcctcgctcactgactc240
gctgcgctcggtcgttcggctgcggcgagcggtatcagctcactcaaaggcggtaatacg300
gttatccacagaatcaggggataacgcaggaaagaacatgtgagcaaaaggccagcaaaa360
ggccaggaaccgtaaaaaggccgcgttgctggcgtttttccataggctccgcccccctga420
cgagcatcacaaaaatcgacgctcaagtcagaggtggcgaaacccgacaggactataaag480
ataccaggcgtttccccctggaagctccctcgtgcgctctcctgttccgaccctgccgct540
taccggatacctgtccgcctttctcccttcgggaagcgtggcgctttctcatagctcacg600
ctgtaggtatctcagttcggtgtaggtcgttcgctccaagctgggctgtgtgcacgaacc660
ccccgttcagcccgaccgctgcgccttatccggtaactatcgtcttgagtccaacccggt720
aagacacgacttatcgccactggcagcagccactggtaacaggattagcagagcgaggta780
tgtaggcggtgctacagagttcttgaagtggtggcctaactacggctacactagaaggac840
agtatttggtatctgcgctctgctgaagccagttaccttcggaaaaagagttggtagctc900
ttgatccggcaaacaaaccaccgctggtagcggtggtttttttgtttgcaagcagcagat960
tacgcgcagaaaaaaaggatctcaagaagatcctttgatcttttctacggggtctgacgc1020
tcagtggaacgaaaactcacgttaagggattttggtcatgagattatcaaaaaggatctt1080
cacctagatccttttaaattaaaaatgaagttttaaatcaatctaaagtatatatgagta1140
aacttggtctgacagttaccaatgcttaatcagtgaggcacctatctcagcgatctgtct1200
atttcgttcatccatagttgcctgactccccgtcgtgtagataactacgatacgggaggg1260
cttaccatctggccccagtgctgcaatgataccgcgagacccacgctcaccggctccaga1320
tttatcagcaataaaccagccagccggaagggccgagcgcagaagtggtcctgcaacttt1380
atccgcctccatccagtctattaattgttgccgggaagctagagtaagtagttcgccagt1440
taatagtttgcgcaacgttgttgccattgctacaggcatcgtggtgtcacgctcgtcgtt1500
tggtatggcttcattcagctccggttcccaacgatcaaggcgagttacatgatcccccat1560
gttgtgcaaaaaagcggttagctccttcggtcctccgatcgttgtcagaagtaagttggc1620
cgcagtgttatcactcatggttatggcagcactgcataattctcttactgtcatgccatc1680
cgtaagatgcttttctgtgactggtgagtactcaaccaagtcattctgagaatagtgtat1740
gcggcgaccgagttgctcttgcccggcgtcaatacgggataataccgcgccacatagcag1800
aactttaaaagtgctcatcattggaaaacgttcttcggggcgaaaactctcaaggatctt1860
accgctgttgagatccagttcgatgtaacccactcgtgcacccaactgatcttcagcatc1920
ttttactttcaccagcgtttctgggtgagcaaaaacaggaaggcaaaatgccgcaaaaaa1980
gggaataagggcgacacggaaatgttgaatactcatactcttcctttttcaatattattg2040
aagcatttatcagggttattgtctcatgagcggatacatatttgaatgtatttagaaaaa2100
taaacaaataggggttccgcgcacatttccccgaaaagtgccacctgacgtctaagaaac2160
cattattatcatgacattaacctataaaaataggcgtatcacgaggccctttcgtc2216
<210>89
<211>2722
<212>dna
<213>人工序列(artificialsequence)
<400>89
gttttagagctagaaatagcaagttaaaataaggctagtccgttatcaacttgaaaaagt60
ggcacccgtgcatgcaggccggggcatatgggaaacagcgcggacgcagcggaatttcca120
atttcatgccgcagccgcctgcgctgttctcatttgcggcttccttgtagagctcagcat180
tattgagtggatgattatattccttttgataggtggtatgttttcgcttgaacttttaaa240
tacagccattgaacatacggttgatttaataactgacaaacatcaccctcttgctaaagc300
ggccaaggacgctgccgccggggctgtttgcgtttttgccgtgatttcgtgtatcattgg360
tttacttatttttttgccaaagctgtaatggctgaaaattcttacatttattttacattt420
ttagaaatgggcgtgaaaaaaagcgcgcgattatgtaaaatataaagtgatagcggtacc480
attataggtaagagaggaatgtacacgctgtttcctgtgtgaaattgttatccgctcaca540
attccacacaacatacgagccggaagcataaagtgtaaagcctggggtgcctaatgagtg600
agctaactcacattaattgcgttgcgctcactgcccgctttccagtcgggaaacctgtcg660
tgccagctgcattaatgaatcggccaacgcgcggggagaggcggtttgcgtattgggcgc720
tcttccgcttcctcgctcactgactcgctgcgctcggtcgttcggctgcggcgagcggta780
tcagctcactcaaaggcggtaatacggttatccacagaatcaggggataacgcaggaaag840
aacatgtgagcaaaaggccagcaaaaggccaggaaccgtaaaaaggccgcgttgctggcg900
tttttccataggctccgcccccctgacgagcatcacaaaaatcgacgctcaagtcagagg960
tggcgaaacccgacaggactataaagataccaggcgtttccccctggaagctccctcgtg1020
cgctctcctgttccgaccctgccgcttaccggatacctgtccgcctttctcccttcggga1080
agcgtggcgctttctcatagctcacgctgtaggtatctcagttcggtgtaggtcgttcgc1140
tccaagctgggctgtgtgcacgaaccccccgttcagcccgaccgctgcgccttatccggt1200
aactatcgtcttgagtccaacccggtaagacacgacttatcgccactggcagcagccact1260
ggtaacaggattagcagagcgaggtatgtaggcggtgctacagagttcttgaagtggtgg1320
cctaactacggctacactagaaggacagtatttggtatctgcgctctgctgaagccagtt1380
accttcggaaaaagagttggtagctcttgatccggcaaacaaaccaccgctggtagcggt1440
ggtttttttgtttgcaagcagcagattacgcgcagaaaaaaaggatctcaagaagatcct1500
ttgatcttttctacggggtctgacgctcagtggaacgaaaactcacgttaagggattttg1560
gtcatgagattatcaaaaaggatcttcacctagatccttttaaattaaaaatgaagtttt1620
aaatcaatctaaagtatatatgagtaaacttggtctgacagttaccaatgcttaatcagt1680
gaggcacctatctcagcgatctgtctatttcgttcatccatagttgcctgactccccgtc1740
gtgtagataactacgatacgggagggcttaccatctggccccagtgctgcaatgataccg1800
cgagacccacgctcaccggctccagatttatcagcaataaaccagccagccggaagggcc1860
gagcgcagaagtggtcctgcaactttatccgcctccatccagtctattaattgttgccgg1920
gaagctagagtaagtagttcgccagttaatagtttgcgcaacgttgttgccattgctaca1980
ggcatcgtggtgtcacgctcgtcgtttggtatggcttcattcagctccggttcccaacga2040
tcaaggcgagttacatgatcccccatgttgtgcaaaaaagcggttagctccttcggtcct2100
ccgatcgttgtcagaagtaagttggccgcagtgttatcactcatggttatggcagcactg2160
cataattctcttactgtcatgccatccgtaagatgcttttctgtgactggtgagtactca2220
accaagtcattctgagaatagtgtatgcggcgaccgagttgctcttgcccggcgtcaata2280
cgggataataccgcgccacatagcagaactttaaaagtgctcatcattggaaaacgttct2340
tcggggcgaaaactctcaaggatcttaccgctgttgagatccagttcgatgtaacccact2400
cgtgcacccaactgatcttcagcatcttttactttcaccagcgtttctgggtgagcaaaa2460
acaggaaggcaaaatgccgcaaaaaagggaataagggcgacacggaaatgttgaatactc2520
atactcttcctttttcaatattattgaagcatttatcagggttattgtctcatgagcgga2580
tacatatttgaatgtatttagaaaaataaacaaataggggttccgcgcacatttccccga2640
aaagtgccacctgacgtctaagaaaccattattatcatgacattaacctataaaaatagg2700
cgtatcacgaggccctttcgtc2722
<210>90
<211>1836
<212>dna
<213>枯草芽孢杆菌(bacillussubtilis)
<400>90
atggcgtttaccatgcagccggtcttaacttcgtcaccgccaatcggagcagaatggcgt60
tacgaagtaaaatatgatgggtaccgctgtattctccgcatccattcttcaggtgtgacc120
ttaaccagcagaaatggtgtggagctcagcagcaccttccctgaaatcacacaattcgcc180
aaaactgcctttcagcacctagaaaaagagcttccgctgacacttgacggtgaaatcgta240
tgtttggtgaatccctgtcgtgcagattttgaacaccttcaagtgcgggggcgtttgaaa300
aggccagacaagattcaggaatcggcaaacgcgcggccgtgctgctttctcgcctttgat360
ttgttagagcgaagcggagaagatgtaacgcttctttcgtatttggaccgcaaaaaatcg420
ctgcgtgaactcatatccgcagcaaagcttcctgcatctccagatccttacgcaaaagaa480
accattcaatccattccctgttatgatcattttgatcaactctgggagatggtaataaaa540
tatgacggagaaggaattgtagcgaagaaaacaaacagcaaatggcttgaaaagaagcgg600
tcgtccgattggctcaaatataaaaactttaaacaagcttatgtttgcataacagggttc660
aaccctaacaacgggtttctgacggtatcggtgttaaaaaacggaataatgacaccgatt720
gcctctgtctcgcacggaatgcgtgatgaggaaaaaagcgctatacgcgagattatggaa780
cagcacggacatcaaacaccctcaggcgaatttacgcttgaaccttcgatttgtgcagct840
gttcaatacttaacaattctccaaggcacattaagagaggtatcgtttatcggtttcgaa900
ttccaaatggactggaccgaatgcacgtatgcgcaagtcatacgacactccaaaccggtt960
cacccaaagctccagttcacaagcctggataaaatcatatttgaaaagaataaaaaaaca1020
aaagaagattttattcagtatatgattgaagtcagtgactacctgctcccatttctgaaa1080
aaccgggctgtcaccgtcatccgctatccgcatggatctaggagcgaatcatttttccaa1140
aaaaacaagccggactatgcgccggattttgttcagtctttctatgatggaagccatgaa1200
catattgtctgtgaggatatgtctacattgctatggctttgcaaccagcttgctttggag1260
tttcacgttccttttcaaaccattaaaagcaggcgtccggctgagattgtcattgattta1320
gacccgccttcgcgagatgactttcttatggccgtgcaggccgcaaatgaactgaagcgg1380
ctgttggattcctttggcatcacatcgtacccgaagctgtccggcaacaaaggcatccag1440
ctttatattcccttgtctcccgaggcatttacgtatgaagaaacacgccaatttacgcag1500
ctgatcgcagagtactgcaccaatgcatttcccgagctctttaccactgaacgcttgatt1560
aaaaacagacactgcaaattgtatcttgattatttgcagcatgcagaaggaaaaacgatt1620
atctgtccctattcaacgagaggaaatgagcttgggactgttgcagccccgctctactgg1680
catgaggtgcaatcatctttaacacccgctctgtttacaattgatacggtcatcgatcga1740
attaagaagcagggctgcccattttttgatttttaccgcaacccgcaagacgaaccgctt1800
agcgctatattgcatcagttgaagaaaaagagctga1836
<210>91
<211>20
<212>dna
<213>人工序列(artificialsequence)
<400>91
gcggtgatagcttctcgttc20
<210>92
<211>20
<212>dna
<213>人工序列(artificialsequence)
<400>92
gctgattcctgcggcaaaag20