一种生物合成牛心果碱的微生物及其方法与流程

本发明属于微生物代谢工程领域，具体涉及改造微生物代谢途径，从单糖或甘油直接合成牛心果碱的方法。

背景技术：

苄基异喹啉类生物碱包含2500多个种类,许多苄基异喹啉类生物碱，如:吗啡、小檗碱和诺斯卡品等在医学领域具有广阔的应用前景与经济地位。牛心果碱是苄基异喹啉类生物碱的共同中间体。

目前已商品化的苄基异喹啉类生物碱主要通过植物提取。由于植物生长周期长及苄基异喹啉类生物碱含量低，利用植物提取苄基异喹啉类生物碱成本较高。通过生物技术手段，实现微生物发酵单糖或甘油合成苄基异喹啉类生物碱共同中间体牛心果碱，可有效的降低成本，为规模化生物合成苄基异喹啉类生物碱提供了可行的道路。

技术实现要素：

本发明的目的在于提供一种微生物体内合成牛心果碱的方法，在微生物内构建一条由单糖或甘油转化到牛心果碱的生物合成途径。

为实现上述目的，本发明提供了在大肠杆菌合成牛心果碱的方法，生物合成路径如图1所示，主要步骤为：

(1)敲除基因构建菌株dy

以大肠杆菌mg1655菌株出发，依次敲除该菌株中七个醛还原酶基因(dkgb、yeae、yqhc、yqhd、dkga、yahk和yjgb),敲除上述7个基因的菌株命名为dy。

(2)构建产牛心果碱工程菌dy1

将来自大肠杆菌的解反馈抑制arog^fbr(3-deoxy-d-arabinoheptulosonate-7-phosphatesynthetase,arog)基因和tyra^fbr(chorismatemutase/prephenatedehydrogenase，tyra)基因及来自酿酒酵母脱羧酶kdc基因，重组到载体pbbr1mcs1上，得到载体pdy001。将来自大肠杆菌的hpabc(4-hydroxyphenylacetate3-hydroxylase，hpabc，由hpab和hpac组成)及经密码子优化人工合成的pseudomonasputida菌dodc(dopadecarboxylase，dodc)、papaversomniferum的cns((s)-norcoclaurinesynthase，cns)、papaversomniferum的6omt((rs)-norcoclaurine6-o-methyltransferase，6omt)、coptisjaponica的4omt(3'-hydroxy-n-methylcoclaurine4'-o-methyltransferase，4omt)和papaversomniferum的cnmt((s)-coclaurinen-methyltransferase，cnmt)基因，重组到载体pet28a(+)上，得到载体pdy002。将经密码子优化人工合成的mycobacteriummarinum菌car(carboxylicacidreductase，car)、bacillussubtilissubsp.subtilisstr.168菌的sfp(phosphopantetheinyltransferase,sfp)和大肠杆菌mg1655的aldh(aldehydedehydrogenase，aldh)基因重组到载体pet28b-psc101，得到载体pdy003。将载体pdy001、pdy002和pdy003转入到敲除七个醛还原酶基因的大肠杆菌dy中，实现上述12个基因在大肠杆菌中的高量表达，得到大肠杆菌工程菌株dy2。敲除七个醛还原酶基因用于阻断中间体4-羟基苯乙醛和3,4-二羟基苯乙醛被还原为副产物4-羟基苯乙醇和3,4-二羟基苯乙醇。表达解除反馈抑制的arog^fbr基因和tyra^fb基因用于加强大肠杆菌胞内对羟基苯丙酮酸和酪氨酸的合成。表达hpab、hpac、kdc、aldh和car用于催化对羟基苯丙酮酸转化为3,4二羟基苯乙醛。表达sfp用于活化car酶。表达hpab、hpac和dodc用于催化酪氨酸转化为3,4二羟基苯乙胺，表达cns、6omt、4omt和cnmt用于随后催化3,4二羟基苯乙醛与3,4二羟基苯乙胺两个产物缩合修饰生成牛心果碱。上述构建的dy1工程菌可有效的将单糖或甘油转化为牛心果碱。

具体而言，本发明一方面涉及一种微生物，其特征在于所述微生物不含有或者敲除dkgb、yeae、yqhc、yqhd、dkga、yahk和yjgb基因，高表达或者导入arog^fbr、tyra^fb、kdc、hpab、hpac、dodc、car、sfp、aldh、cns、4omt、6omt和cnmt基因。

进一步的，所述dkgb基因具有seqidno：1所示的核苷酸序列或者所述dkgb基因编码seqidno：2所示的氨基酸序列；

所述yeae基因具有seqidno：3所示的核苷酸序列或者所述yeae基因编码seqidno：4所示的氨基酸序列；

所述yqhc基因具有seqidno：5所示的核苷酸序列或者所述yqhc基因编码seqidno：6所示的氨基酸序列；

所述yqhd基因具有seqidno：7所示的核苷酸序列或者所述yqhd基因编码seqidno：8所示的氨基酸序列；

所述dkga基因具有seqidno：9所示的核苷酸序列或者所述dkga基因编码seqidno：10所示的氨基酸序列；

所述yahk基因具有seqidno：11所示的核苷酸序列或者所述yahk基因编码seqidno：12所示的氨基酸序列；

所述yjgb基因具有seqidno：13所示的核苷酸序列或者所述yjgb基因编码seqidno：14所示的氨基酸序列；

所述arog^fbr基因具有seqidno：15所示的核苷酸序列或者所述arog^fbr基因编码seqidno：16所示的氨基酸序列；

所述tyra^fb基因具有seqidno：17所示的核苷酸序列或者所述tyra^fb基因编码seqidno：18所示的氨基酸序列；

所述kdc基因具有seqidno：19所示的核苷酸序列或者所述kdc基因编码seqidno：20所示的氨基酸序列；

所述dodc基因具有seqidno：21所示的核苷酸序列或者所述dodc基因编码seqidno：22所示的氨基酸序列；

所述cns基因具有seqidno：23所示的核苷酸序列或者所述cns基因编码seqidno：24所示的氨基酸序列；

所述6omt基因具有seqidno：25所示的核苷酸序列或者所述6omt基因编码seqidno：26所示的氨基酸序列；

所述4omt基因具有seqidno：27所示的核苷酸序列或者所述4omt基因编码seqidno：28所示的氨基酸序列；

所述cnmt基因具有seqidno：29所示的核苷酸序列或者所述nmch基因编码seqidno：30所示的氨基酸序列；

所述hpab基因具有seqidno：31所示的核苷酸序列或者所述hpab基因编码seqidno：32所示的氨基酸序列；

所述hpac基因具有seqidno：33所示的核苷酸序列或者所述hpac基因编码seqidno：34所示的氨基酸序列；

所述car基因具有seqidno：35所示的核苷酸序列或者所述car基因编码seqidno：36所示的氨基酸序列；

所述sfp基因具有seqidno：37所示的核苷酸序列或者所述sfp基因编码seqidno：38所示的氨基酸序列；

所述aldh基因具有seqidno：39所示的核苷酸序列或者所述aldh基因编码seqidno：40所示的氨基酸序列。

在本发明的一个优选实施方式中，微生物为大肠杆菌。

本发明另一方面还涉及上述微生物在生物合成牛心果碱中的应用。

作为微生物发酵发酵单糖或甘油合成牛心果碱的方法，所述的单糖为葡萄糖。

本发明的优点是在微生物中引入外源基因构建一条合成牛心果碱的代谢途径，最终实现以单糖或甘油为原料合成牛心果碱的目的，相比较与植物提取或化学合成可有效的降低成本。同时，微生物生长速度快，遗传操作技术成熟，且发酵过程不会对环境造成污染破坏。

附图说明

图1.牛心果碱生物合成路径图；

图2.大肠杆菌工程菌dy1和dy2发酵产物gc-ms检测结果。1号峰为内标4,4'-二甲氧基二苯甲酮,2号峰为产物牛心果碱。

图3，图2中发酵产物2号峰的离子碎片(上)与标准牛心果碱离子碎片(下)比较图。

具体实施方式

以下实施例用于进一步说明本发明，但不应理解为对本发明的限制。

实施例1构建产牛心果碱大肠杆菌工程菌

1.基因敲除菌株dy的构建

以pkd4质粒为模板，pcr扩增打靶片段,该片段包含(kan^r序列和与待敲除目的基因两端互补的同源臂)；将扩增的打靶片段电转到带有pkd46质粒的大肠杆菌中。利用pkd46质粒上带有的red同源重组系统，使待敲除目的基因与打靶片段进行重组，目的基因被抗性基因取代。随后将温敏质粒pcp20导入菌株，利用该质粒上的flp翻转酶去除目的菌株染色体上的抗性基因。利用上述方法依次敲除大肠杆菌mg1655菌株中的dkgb、yeae、yqhc、yqhd、dkga、yahk和yjgb基因，获得大肠杆菌dy。所述dkgb基因具有seqidno：1所示的核苷酸序列；所述dkgb基因具有与seqidno：2所示的氨基酸序列；所述yeae基因具有seqidno：3所示的核苷酸序列；所述yeae基因具有与seqidno：4所示的氨基酸序列；所述yqhc基因具有seqidno：5所示的核苷酸序列；所述yqhc基因具有与seqidno：6所示的氨基酸序列；所述yqhd基因具有seqidno：7所示的核苷酸序列；所述yqhd基因具有与seqidno：8所示的氨基酸序列；所述dkga基因具有seqidno：9所示的核苷酸序列；所述dkga基因具有与seqidno：10所示的氨基酸序列；所述yahk基因具有seqidno：11所示的核苷酸序列；所述yahk基因具有与seqidno：12所示的氨基酸序列；所述yjgb基因具有seqidno：13所示的核苷酸序列；所述yjgb基因具有与seqidno：14所示的氨基酸序列；敲除基因用的引物为：

dkgb-f:atggctatccctgcatttggtttaggtactttccgtctgagtgtaggctggagctgcttc

dkgb-r:ttaatcccattcaggagccagaccttccgggctaaccaggcggcatatgaatatcctcctta

yeae-f:atgcaacaaaaaatgattcaatttagtggcgatgtctcactggtgtaggctggagctgcttc

yeae-r:tcacaccatatccagcgcagtttttccttttggtgccggatatcatatgaatatcctcctta

yqhc-f:atgctacaaaattgcgcacaatcaaattgccgcattattcctaagtgtaggctggagctgcttc

yqhc-r:ttaattcccctgcatcgcccgcattcttgccgcatcttcccccatatgaatatcctcctta

yqhd-f:atgaacaactttaatctgcacaccccaacccgcattctgttgtgtaggctggagctgcttc

yqhd-r:ttagcgggcggcttcgtatatacggcggctgacatccaacgcatatgaatatcctcctta

dkga-f：atggctaatccaaccgttattaagctacaggatggcaatgtcgtgtaggctggagctgcttc

dkga-r：ttagccgccgaactggtcaggatcgggaccgagacgcttgccatatgaatatcctcctta

yahk-f:atgaagatcaaagctgttggtgcatattccgctaaacaaccgtgtaggctggagctgcttc

yahk-r:tcagtctgttagtgtgcgattatcgataacaaaacgatattcatatgaatatcctcctta

yjgb-f:atgtcgatgataaaaagctatgccgcaaaagaagcgggcggcgtgtaggctggagctgcttc

yjgb-r:tcaaaaatcggctttcaacaccacgcggtaacgcgccttaccgtcatatgaatatcctcctta

2.载体pdy001的构建

解除反馈抑制的arog^fbr和tyra^fbr基因委托金唯智生物科技有限公司合成，以合成的基因为模板利用引物arog^fbr-xbai(gtatctagaaagaggagatataatgaattatcagaacgacgatttacgc)和arog^fbr-spei-bamhi(tatggatccactagtttacccgcgacgcgctttta)pcr扩增arog^fbr基因，随后将扩增的片段以xbai和bamhi插入到pet28a(+)中得到载体pdy1。以合成的基因为模板，利用引物tyra^fbrr-xbai(gtatctagaaagaggagatataatggttgctgaattgaccgcat)和tyra^fbr-spei-sali(atatgtcgacactagtttactggcgattgtcattcgcc)pcr扩增来tyra^fbr基因，随后将扩增的片段以xbai和sali双酶切，插入到以spei和sali双酶切的载体pdy1上，中得到载体pdy2。利用引物kdc-xbai(atctctagatttaagaaggagatataatggcacctgttacaattgaaaagttc)和kdc-nhei-bamhi(tctggatccgctagcctattttttatttcttttaagtgccgctg)pcr扩增来自酿酒酵母菌yph499的kdc基因，随后将扩增的片段以xbai和bamhi双酶切,将其插入以xbai和bamhi双酶切的载体pbbr1mcs1上，得到载体pdy3。利用xbai和sali双酶切pdy2得到arog^fbr和tyra^fbr表达框，将其插入以nhei和xhoi双酶切的载体pdy3，得到载体pdy001。所述arog^fbr基因具有seqidno：15所示的核苷酸序列；所述arog^fbr基因具有与seqidno：16所示的氨基酸序列；所述tyra^fb基因具有seqidno：17所示的核苷酸序列编码；所述tyra^fb基因具有seqidno：18所示的氨基酸序列；所述kdc基因具有seqidno：19所示的核苷酸序列编码；所述kdc基因具有seqidno：20所示的氨基酸序列。

3.载体pdy002的构建

dodc、cns、6omt、4omt和cnmt基因委托金唯智生物科技有限公司合成。以合成的基因为模板，利用引物dodc-xbai(tagtctagatttaagaaggagatataatgacccccgaacagttccg)/dodc-spei-bamhi(acaggatccactagtttagctgccgcctttaatcacgt)、cns-xbai(tactctagatttaagaaggagatataatgcgtaaagtgatcaaatatgatatgg)/cns-spei-bamhi(tcaggatccactagtttagctgcccagtaaacgaggaa)、6omt-xbai(ttgtctagatttaagaaggagatataatggagaccgttagcaaaattgacc)/6omt-spei-bamhi(gcaggatccactagtttagtacggatatgcttcaatcacgct)、4omt-xbai(tactctagatttaagaaggagatataatggccttccatggcaaggac)/4omt-spei-bamhi(tcaggatccactagtttaaggaaacacctcaatcacgctct)、cnmt-xbai(atctctagatttaagaaggagatataatgcagttaaaagaaaaagaggagctg)/cnmt-spei-bamhi(agaggatccactagtttattttttcttaaacagcagatgggttaac)，分别pcr扩增dodc、cns、6omt、4omt和cnmt基因，随后将扩增的片段以xbai和bamhi插入到pet28a(+)中，依次得到载体pdy4、pdy5、pdy6、pdy7和pdy8。利用xbai和xhoi双酶切pdy5得到cns表达框，将其插入以spei和xhoi双酶切的载体pdy4上，得到载体pdy9。利用xbai和xhoi双酶切pdy6得到6omt表达框，将其插入以spei和xhoi双酶切的载体pdy9上，得到载体pdy10。利用xbai和xhoi双酶切pdy7得到4omt表达框，将其插入以spei和xhoi双酶切的载体pdy10上，得到载体pdy11。利用xbai和xhoi双酶切pdy8得到cnmt表达框，将其插入以spei和xhoi双酶切的载体pdy11上，得到载体pdy12。以大肠杆菌mg1655的基因组为模板，利用引物hpabc-xbai(tagtctagatttaagaaggagatataatgaaaccagaagatttccgcg)和hpabc-xhoi(aacgctcgagttaaatcgcagcttccatttcca),pcr扩增hpabc基因(包含hpab和hpac两个基因)，随后将扩增的片段以xbai和xhoi双酶切,将其插入以spei和xhoi双酶切的载体pdy12上，得到载体pdy002。所述dodc基因具有seqidno：21所示的核苷酸序列；所述dodc基因具有与seqidno：22所示的氨基酸序列；所述cns基因具有seqidno：23所示的核苷酸序列；所述cns基因具有与seqidno：24所示的氨基酸序列；所述6omt基因具有seqidno：25所示的核苷酸序列；所述6omt基因具有与seqidno：26所示的氨基酸序列；所述4omt基因具有seqidno：27所示的核苷酸序列；所述4omt基因具有与seqidno：28所示的氨基酸序列；所述cnmt基因具有seqidno：29所示的核苷酸序列；所述cnmt基因具有与seqidno：30所示的氨基酸序列；所述hpab基因具有seqidno：31所示的核苷酸序列；所述hpab基因具有与seqidno：32所示的氨基酸序列；所述hpac基因具有seqidno：33所示的核苷酸序列；所述hpac基因具有与seqidno：34所示的氨基酸序列；

4.载体pdy003的构建

car基因委托金唯智生物科技有限公司合成。以合成的基因为模板，利用引物car-xbai(atctctagatttaagaaggagatataatgagtccgatcacacgcgag)/car-spei-bamhi(agaggatccactagtttacagcaggcctaacaggcg)pcr扩增car基因，随后将扩增的片段以xbai和bamhi双酶切，插入到以xbai和bamhi双酶切的pet28a(+)中，得到载体pdy13。以芽孢杆菌bacillussubtilissubsp.subtilisstr.168的基因组为模板，利用引物sfp-xbai(gtctctagatttaagaaggagatataatgaagatttacggaatttatatggaccg)/sfp-spei-bamhi(tgaggatccactagtttataaaagctcttcgtacgagaccattgt)pcr扩增sfp基因，随后将扩增的片段以xbai和bamhi双酶切，插入到以xbai和bamhi双酶切的pet28a(+)中，得到载体pdy14。以大肠杆菌mg1655的基因组为模板，利用引物aldh-xbai(atctctagatttaagaaggagatataatgaattttcatcatctggcttactg)/aldh-nhei-bamhi(tcaggatccgctagctcaggcctccaggctatcc)pcr扩增aldh基因，随后将扩增的片段以xbai和bamhi双酶切，插入到以xbai和bamhi双酶切的pet28a(+)中，得到载体pdy15。利用xbai和xhoi双酶切pdy14得到sfp表达框，将其插入以spei和xhoi双酶切的载体pdy13上，得到载体pdy16。利用xbai和xhoi双酶切pdy15得到aldh表达框，将其插入以spei和xhoi双酶切的载体pdy16上，得到载体pdy17。利用xbai和xhoi双酶切pdy17得到car、sfp和aldh表达框，将其插入以xbai和xhoi双酶切的载体pet28b-psc101上，得到载体pdy003。所述car基因具有seqidno：35所示的核苷酸序列；所述car基因具有与seqidno：36所示的氨基酸序列；所述sfp基因具有seqidno：37所示的核苷酸序列；所述sfp基因具有与seqidno：38所示的氨基酸序列；所述aldh基因具有seqidno：39所示的核苷酸序列；所述aldh基因具有与seqidno：40所示的氨基酸序列；

5.将重组载体pdy001和pdy002同时导入大肠杆菌dy中，得到dy1工程菌。该工程菌可实现arog^fbr、tyra^fbr、kdc、hpabc、dodc、cns、6omt、4omt和cnmt基因的高表达，可有效的将单糖或甘油转化为牛心果碱。

5.将重组载体pdy001、pdy002和pdy003同时导入大肠杆菌dy中，得到dy2工程菌。该工程菌可实现arog^fbr、tyra^fbr、kdc、hpabc、dodc、cns、6omt、4omt、cnmt、car、sfp和aldh基因的高表达。相较于dy1工程菌，进一步实现car、sfp和aldh基因高效表达的dy2工程菌，可提升的将单糖或甘油转化为牛心果碱的生物合成效率。

实施例2大肠杆菌工程菌dy1生产牛心果碱的发酵实验

1、将37度lb中过夜培养的大肠杆菌工程菌dy1，接种到50mlm9发酵培养基中(包含2％葡萄糖)，进行30度摇瓶发酵，待菌体od600为0.8左右，加入0.1mmiptg进行目的基因的诱导表达。

2、发酵培养24小时后收集菌体，超声波破细胞，利用氯仿萃取产物。

3、利用旋转蒸发仪将溶剂氯仿旋干，再用一定量的氯仿溶解产物。

4、gc-ms(气相色谱-质谱联用仪)检测样品。

5、实验结果：大肠杆菌工程菌利用葡萄糖合成牛心果碱的gc-ms检测结果如图2a所示，牛心果碱gc-ms鉴定如图3所示，表明菌株dy1可有效利用葡萄糖或甘油合成牛心果碱。

实施例3大肠杆菌工程菌dy2生产牛心果碱的发酵实验

1、将37度lb中过夜培养的大肠杆菌工程菌dy2，接种到50mlm9发酵培养基中(包含2％葡萄糖)，进行30度摇瓶发酵，待菌体od600为0.8左右，加入0.1mmiptg进行目的基因的诱导表达。

2、发酵培养24小时后收集菌体，超声波破细胞，利用氯仿萃取产物。

3、利用旋转蒸发仪将溶剂氯仿旋干，再用一定量的氯仿溶解产物。

4、gc-ms(气相色谱-质谱联用仪)检测样品。

5、实验结果：大肠杆菌工程菌dy2利用葡萄糖合成牛心果碱的gc-ms检测结果如图2b，表明菌株dy2可有效利用葡萄糖或甘油合成牛心果碱。

以上所述仅是本发明的优选实施方案，应当指出，对于本技术领域的技术人员，在不脱离本分明原理的前提下，还可以做出适当的改进，这些改进也应视为本发明的保护范围。

序列表

<110>赣南师范大学

<120>一种生物合成牛心果碱的微生物及其方法

<160>78

<170>siposequencelisting1.0

<210>1

<211>804

<212>dna

<213>unkown

<400>1

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gggctgacgcgtgagatcggtatttccaacttcacgatcccgttgatggaaaaagcgatt420

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aaccgtaaagtggttgcctgggctaaacagcacggcatccatattacttcctatatgacg540

ctggcgtatggtaaggccctgaaagatgaggttattgctcgtatcgcagctaaacacaat600

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<213>unkown

<400>2

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151015

valvalileserservalilethralaleugluleuglytyrargala

202530

ileaspthralaglniletyraspasnglualaalavalglyglnala

354045

ilealagluserglyvalproarghisgluleutyrilethrthrlys

505560

iletrpilegluasnleuserlysasplysleuileproserleulys

65707580

gluserleuglnlysleuargthrasptyrvalaspleuthrleuile

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histrpproserproasnaspgluvalservalglugluphemetgln

100105110

alaleuleuglualalyslysglnglyleuthrarggluileglyile

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serasnphethrileproleumetglulysalailealaalavalgly

130135140

alagluasnilealathrasnglnilegluleuserprotyrleugln

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asnarglysvalvalalatrpalalysglnhisglyilehisilethr

165170175

sertyrmetthrleualatyrglylysalaleulysaspgluvalile

180185190

alaargilealaalalyshisasnalathrproalaglnvalileleu

195200205

alatrpalametglygluglytyrservalileproserserthrlys

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arglysasnleugluserasnleulysalaglnasnleuglnleuasp

225230235240

alagluasplyslysalailealaalaleuaspcysasnaspargleu

245250255

valserprogluglyleualaproglutrpasp

260265

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acatggtatatgggcgaagatgccagtcagcgcaaaacagaagttgctgcactacgcgcg120

ggcattgaactcggtttaaccctcattgataccgccgaaatgtatgccgatggcggtgcc180

gaaaaggtggttggggaagcattaaccggtctgcgagagaaggtctttctcgtctctaaa240

gtctatccgtggaatgctggcgggcaaaaagcgataaatgcatgcgaagccagtttacgc300

cgtctcaatactgattatctcgatctttacttattacactggtctggcagtttcgctttt360

gaagagactgtcgcagcgatggaaaaattgatcgcccagggaaaaatccgccgctggggc420

gtttctaaccttgattatgctgatatgcaggaactctggcagctgccggggggaaatcag480

tgtgccactaatcaggtgctttaccatctcggttcacgaggaattgagtacgatctactc540

ccctggtgccagcaacagcagatgccggtgatggcttacagtccgttagcccaggccggg600

cggttgcgcaatggactgttaaaaaacgcggtagtcaacgaaattgcacatgctcacaat660

atcagcgcggcacaagtattgttggcgtgggtgatcagtcatcagggtgtgatggcgatt720

ccaaaagcggccacgattgcccatgtccaacaaaatgcggctgtgcttgaggtcgaactt780

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<211>284

<212>prt

<213>unkown

<400>4

metglnglnlysmetileglnpheserglyaspvalserleuproala

151015

valglyglnglythrtrptyrmetglygluaspalaserglnarglys

202530

thrgluvalalaalaleuargalaglyilegluleuglyleuthrleu

354045

ileaspthralaglumettyralaaspglyglyalaglulysvalval

505560

glyglualaleuthrglyleuargglulysvalpheleuvalserlys

65707580

valtyrprotrpasnalaglyglyglnlysalaileasnalacysglu

859095

alaserleuargargleuasnthrasptyrleuaspleutyrleuleu

100105110

histrpserglyserphealapheglugluthrvalalaalametglu

115120125

lysleuilealaglnglylysileargargtrpglyvalserasnleu

130135140

asptyralaaspmetglngluleutrpglnleuproglyglyasngln

145150155160

cysalathrasnglnvalleutyrhisleuglyserargglyileglu

165170175

tyraspleuleuprotrpcysglnglnglnglnmetprovalmetala

180185190

tyrserproleualaglnalaglyargleuargasnglyleuleulys

195200205

asnalavalvalasngluilealahisalahisasnileseralaala

210215220

glnvalleuleualatrpvalileserhisglnglyvalmetalaile

225230235240

prolysalaalathrilealahisvalglnglnasnalaalavalleu

245250255

gluvalgluleuserseralagluleualametleuasplysalatyr

260265270

proalaprolysglylysthralaleuaspmetval

275280

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<213>unkown

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atgctacaaaattgcgcacaatcaaattgccgcattattcctaagaaattacgcgatatg60

aaacgtgaagagatttgccgcttgctggcggataaagttaataaactgaaaaataaagaa120

aatagtttgtcaggactgttgcccgatgtgcgtttgttgtatggcgagacgcctttcgca180

cgtacaccggtgatgtacgagcctggcatcataattctcttttccgggcataaaatcggt240

tatatcaatgaacgcgtgtttcgttatgatgccaatgaatacctgctgctgacggtgccg300

ttgccgtttgagtgcgaaacctatgccacgtcagaggtgccgctggcagggttgcgtctc360

aatgtcgatattttgcagttacaggaactgttgatggacattggcgaagatgagcatttc420

cagccgtcgatggcagccagcgggattaactccgccacgttatcagaagagattttatgc480

gcggcggagcggttactcgacgtgatggagcgaccactggatgcgcgtattctcggcaaa540

cagatcatccgcgaaattctgtactacgtgctgaccggaccttgcggcggcgcgttactg600

gcgctggtcagtcgccagactcacttcagtctgattagccgcgtgctgaaacggattgag660

aataaatacaccgaaaacctgagcgtcgagcaactggcggcagaagccaacatgagcgta720

tcggcgttccaccataattttaagtctgtcaccagtacctcgccgttgcagtatttgaag780

aattaccgtctgcataaggcgcggatgatgatcatccatgacggcatgaaggccagcgca840

gcagcgatgcgcgtcggctatgaaagcgcatcgcaatttagccgtgagtttaaacgttac900

ttcggtgtgacgccgggggaagatgcggcaagaatgcgggcgatgcaggggaattaa957

<210>6

<211>318

<212>prt

<213>unkown

<400>6

metleuglnasncysalaglnserasncysargileileprolyslys

151015

leuargaspmetlysargglugluilecysargleuleualaasplys

202530

valasnlysleulysasnlysgluasnserleuserglyleuleupro

354045

aspvalargleuleutyrglygluthrprophealaargthrproval

505560

mettyrgluproglyileileileleupheserglyhislysilegly

65707580

tyrileasngluargvalpheargtyraspalaasnglutyrleuleu

859095

leuthrvalproleupropheglucysgluthrtyralathrserglu

100105110

valproleualaglyleuargleuasnvalaspileleuglnleugln

115120125

gluleuleumetaspileglygluaspgluhispheglnprosermet

130135140

alaalaserglyileasnseralathrleuserglugluileleucys

145150155160

alaalagluargleuleuaspvalmetgluargproleuaspalaarg

165170175

ileleuglylysglnileilearggluileleutyrtyrvalleuthr

180185190

glyprocysglyglyalaleuleualaleuvalserargglnthrhis

195200205

pheserleuileserargvalleulysargilegluasnlystyrthr

210215220

gluasnleuservalgluglnleualaalaglualaasnmetserval

225230235240

seralaphehishisasnphelysservalthrserthrserproleu

245250255

glntyrleulysasntyrargleuhislysalaargmetmetileile

260265270

hisaspglymetlysalaseralaalaalametargvalglytyrglu

275280285

seralaserglnpheserarggluphelysargtyrpheglyvalthr

290295300

proglygluaspalaalaargmetargalametglnglyasn

305310315

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<211>1164

<212>dna

<213>unkown

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atgaacaactttaatctgcacaccccaacccgcattctgtttggtaaaggcgcaatcgct60

ggtttacgcgaacaaattcctcacgatgctcgcgtattgattacctacggcggcggcagc120

gtgaaaaaaaccggcgttctcgatcaagttctggatgccctgaaaggcatggacgtgctg180

gaatttggcggtattgagccaaacccggcttatgaaacgctgatgaacgccgtgaaactg240

gttcgcgaacagaaagtgactttcctgctggcggttggcggcggttctgtactggacggc300

accaaatttatcgccgcagcggctaactatccggaaaatatcgatccgtggcacattctg360

caaacgggcggtaaagagattaaaagcgccatcccgatgggctgtgtgctgacgctgcca420

gcaaccggttcagaatccaacgcaggcgcggtgatctcccgtaaaaccacaggcgacaag480

caggcgttccattctgcccatgttcagccggtatttgccgtgctcgatccggtttatacc540

tacaccctgccgccgcgtcaggtggctaacggcgtagtggacgcctttgtacacaccgtg600

gaacagtatgttaccaaaccggttgatgccaaaattcaggaccgtttcgcagaaggcatt660

ttgctgacgctaatcgaagatggtccgaaagccctgaaagagccagaaaactacgatgtg720

cgcgccaacgtcatgtgggcggcgactcaggcgctgaacggtttgattggcgctggcgta780

ccgcaggactgggcaacgcatatgctgggccacgaactgactgcgatgcacggtctggat840

cacgcgcaaacactggctatcgtcctgcctgcactgtggaatgaaaaacgcgataccaag900

cgcgctaagctgctgcaatatgctgaacgcgtctggaacatcactgaaggttccgatgat960

gagcgtattgacgccgcgattgccgcaacccgcaatttctttgagcaattaggcgtgccg1020

acccacctctccgactacggtctggacggcagctccatcccggctttgctgaaaaaactg1080

gaagagcacggcatgacccaactgggcgaaaatcatgacattacgttggatgtcagccgc1140

cgtatatacgaagccgcccgctaa1164

<210>8

<211>387

<212>prt

<213>unkown

<400>8

metasnasnpheasnleuhisthrprothrargileleupheglylys

151015

glyalailealaglyleuarggluglnileprohisaspalaargval

202530

leuilethrtyrglyglyglyservallyslysthrglyvalleuasp

354045

glnvalleuaspalaleulysglymetaspvalleuglupheglygly

505560

ilegluproasnproalatyrgluthrleumetasnalavallysleu

65707580

valarggluglnlysvalthrpheleuleualavalglyglyglyser

859095

valleuaspglythrlyspheilealaalaalaalaasntyrproglu

100105110

asnileaspprotrphisileleuglnthrglyglylysgluilelys

115120125

seralaileprometglycysvalleuthrleuproalathrglyser

130135140

gluserasnalaglyalavalileserarglysthrthrglyasplys

145150155160

glnalaphehisseralahisvalglnprovalphealavalleuasp

165170175

provaltyrthrtyrthrleuproproargglnvalalaasnglyval

180185190

valaspalaphevalhisthrvalgluglntyrvalthrlysproval

195200205

aspalalysileglnaspargphealagluglyileleuleuthrleu

210215220

ilegluaspglyprolysalaleulysgluprogluasntyraspval

225230235240

argalaasnvalmettrpalaalathrglnalaleuasnglyleuile

245250255

glyalaglyvalproglnasptrpalathrhismetleuglyhisglu

260265270

leuthralamethisglyleuasphisalaglnthrleualaileval

275280285

leuproalaleutrpasnglulysargaspthrlysargalalysleu

290295300

leuglntyralagluargvaltrpasnilethrgluglyseraspasp

305310315320

gluargileaspalaalailealaalathrargasnphepheglugln

325330335

leuglyvalprothrhisleuserasptyrglyleuaspglyserser

340345350

ileproalaleuleulyslysleuglugluhisglymetthrglnleu

355360365

glygluasnhisaspilethrleuaspvalserargargiletyrglu

370375380

alaalaarg

385

<210>9

<211>828

<212>dna

<213>unkown

<400>9

atggctaatccaaccgttattaagctacaggatggcaatgtcatgccccagctgggactg60

ggcgtctggcaagcaagtaatgaggaagtaatcaccgccattcaaaaagcgttagaagtg120

ggttatcgctcgattgataccgccgcggcctacaagaacgaagaaggtgtcggcaaagcc180

ctgaaaaatgcctcagtcaacagagaagaactgttcatcaccactaagctgtggaacgac240

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atcgacctctacttaatgcactggcccgttcccgctatcgaccattatgtcgaagcatgg360

aaaggcatgatcgaattgcaaaaagagggattaatcaaaagcatcggcgtgtgcaacttc420

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atcgaacttcatccgctgatgcaacaacgccagctacacgcctggaacgcgacacacaaa540

atccagaccgaatcctggagcccattagcgcaaggagggaaaggcgttttcgatcagaaa600

gtcattcgcgatctggcagataaatacggcaaaaccccggcgcagattgttatccgctgg660

catctggatagcggcctggtggtgatcccgaaatcggtcacaccttcacgtattgccgaa720

aactttgatgtctgggatttccgtctcgacaaagacgaactcggcgaaattgcaaaactc780

gatcagggcaagcgtctcggtcccgatcctgaccagttcggcggctaa828

<210>10

<211>275

<212>prt

<213>unkown

<400>10

metalaasnprothrvalilelysleuglnaspglyasnvalmetpro

151015

glnleuglyleuglyvaltrpglnalaserasnglugluvalilethr

202530

alaileglnlysalaleugluvalglytyrargserileaspthrala

354045

alaalatyrlysasnglugluglyvalglylysalaleulysasnala

505560

servalasnargglugluleupheilethrthrlysleutrpasnasp

65707580

asphislysargproargglualaleuleuaspserleulyslysleu

859095

glnleuasptyrileaspleutyrleumethistrpprovalproala

100105110

ileasphistyrvalglualatrplysglymetilegluleuglnlys

115120125

gluglyleuilelysserileglyvalcysasnpheglnilehishis

130135140

leuglnargleuileaspgluthrglyvalthrprovalileasngln

145150155160

ilegluleuhisproleumetglnglnargglnleuhisalatrpasn

165170175

alathrhislysileglnthrglusertrpserproleualaglngly

180185190

glylysglyvalpheaspglnlysvalileargaspleualaasplys

195200205

tyrglylysthrproalaglnilevalileargtrphisleuaspser

210215220

glyleuvalvalileprolysservalthrproserargilealaglu

225230235240

asnpheaspvaltrpasppheargleuasplysaspgluleuglyglu

245250255

ilealalysleuaspglnglylysargleuglyproaspproaspgln

260265270

pheglygly

275

<210>11

<211>1050

<212>dna

<213>unkown

<400>11

atgaagatcaaagctgttggtgcatattccgctaaacaaccacttgaaccgatggatatc60

acccggcgtgaaccgggaccgaatgatgtcaaaatcgaaatcgcttactgtggcgtttgc120

cattccgatctccaccaggtccgttccgagtgggcggggacggtttacccctgcgtgccg180

ggtcatgaaattgtggggcgtgtggtagccgttggtgatcaggtagaaaaatatgcgccg240

ggcgatctggtcggtgtcggctgcattgtcgacagttgtaaacattgcgaagagtgtgaa300

gacgggttggaaaactactgtgatcacatgaccggcacctataactcgccgacgccggac360

gaaccgggccatactctgggcggctactcacaacagatcgtcgttcatgagcgatatgtt420

ctgcgtattcgtcacccgcaagagcagctggcggcggtggctcctttgttgtgtgcaggg480

atcaccacgtattcgccgctacgtcactggcaggccgggccgggtaaaaaagtgggcgtg540

gtcggcatcggcggtctgggacatatggggattaagctggcccacgcgatgggggcacat600

gtggtggcatttaccacttctgaggcaaaacgcgaagcggcaaaagccctgggggccgat660

gaagttgttaactcacgcaatgccgatgagatggcggctcatctgaagagtttcgatttc720

attttgaatacagtagctgcgccacataatctcgacgattttaccaccttgctgaagcgt780

gatggcaccatgacgctggttggtgcgcctgcgacaccgcataaatcgccggaagttttc840

aacctgatcatgaaacgccgtgcgatagccggttctatgattggcggcattccagaaact900

caggagatgctcgatttttgcgccgaacatggcatcgtggctgatatagagatgattcgg960

gccgatcaaattaatgaagcctatgagcgaatgctgcgcggtgatgtgaaatatcgtttt1020

gttatcgataatcgcacactaacagactga1050

<210>12

<211>349

<212>prt

<213>unkown

<400>12

metlysilelysalavalglyalatyrseralalysglnproleuglu

151015

prometaspilethrargarggluproglyproasnaspvallysile

202530

gluilealatyrcysglyvalcyshisseraspleuhisglnvalarg

354045

serglutrpalaglythrvaltyrprocysvalproglyhisgluile

505560

valglyargvalvalalavalglyaspglnvalglulystyralapro

65707580

glyaspleuvalglyvalglycysilevalaspsercyslyshiscys

859095

gluglucysgluaspglyleugluasntyrcysasphismetthrgly

100105110

thrtyrasnserprothrproaspgluproglyhisthrleuglygly

115120125

tyrserglnglnilevalvalhisgluargtyrvalleuargilearg

130135140

hisproglngluglnleualaalavalalaproleuleucysalagly

145150155160

ilethrthrtyrserproleuarghistrpglnalaglyproglylys

165170175

lysvalglyvalvalglyileglyglyleuglyhismetglyilelys

180185190

leualahisalametglyalahisvalvalalaphethrthrserglu

195200205

alalysargglualaalalysalaleuglyalaaspgluvalvalasn

210215220

serargasnalaaspglumetalaalahisleulysserpheaspphe

225230235240

ileleuasnthrvalalaalaprohisasnleuaspaspphethrthr

245250255

leuleulysargaspglythrmetthrleuvalglyalaproalathr

260265270

prohislysserprogluvalpheasnleuilemetlysargargala

275280285

ilealaglysermetileglyglyileprogluthrglnglumetleu

290295300

aspphecysalagluhisglyilevalalaaspileglumetilearg

305310315320

alaaspglnileasnglualatyrgluargmetleuargglyaspval

325330335

lystyrargphevalileaspasnargthrleuthrasp

340345

<210>13

<211>1020

<212>dna

<213>unkown

<400>13

atgtcgatgataaaaagctatgccgcaaaagaagcgggcggcgaactggaagtttatgag60

tacgatcccggtgagctgaggccacaagatgttgaagtgcaggtggattactgcgggatc120

tgccattccgatctgtcgatgatcgataacgaatggggattttcacaatatccgctggtt180

gccgggcatgaggtgattgggcgcgtggtggcactcgggagcgccgcgcaggataaaggt240

ttgcaggtcggtcagcgtgtcgggattggctggacggcgcgtagctgtggtcactgcgac300

gcctgtattagcggtaatcagatcaactgcgagcaaggtgcggtgccgacgattatgaat360

cgcggtggctttgccgagaagttgcgtgcggactggcaatgggtgattccactgccagaa420

aatattgatatcgagtccgccgggccgctgttgtgcggcggtatcacggtctttaaacca480

ctgttgatgcaccatatcactgctaccagccgcgttggggtaattggtattggcgggctg540

gggcatatcgctataaaacttctgcacgcaatgggatgcgaggtgacagcctttagttct600

aatccggcgaaagagcaggaagtgctggcgatgggtgccgataaagtggtgaatagccgc660

gatccgcaggcactgaaagcactggcggggcagtttgatctcattatcaacaccgtcaac720

gtcagcctcgactggcagccctattttgaggcgctgacctatggcggtaatttccatacg780

gtcggtgcggttctcacgccgctgtctgttccggcctttacgttaattgcgggcgatcgc840

agcgtctctggttctgctaccggcacgccttatgagctgcgtaagctgatgcgttttgcc900

gcccgcagcaaggttgcgccgaccaccgaactgttcccgatgtcgaaaattaacgacgcc960

atccagcatgtgcgcgacggtaaggcgcgttaccgcgtggtgttgaaagccgatttttga1020

<210>14

<211>339

<212>prt

<213>unkown

<400>14

metsermetilelyssertyralaalalysglualaglyglygluleu

151015

gluvaltyrglutyraspproglygluleuargproglnaspvalglu

202530

valglnvalasptyrcysglyilecyshisseraspleusermetile

354045

aspasnglutrpglypheserglntyrproleuvalalaglyhisglu

505560

valileglyargvalvalalaleuglyseralaalaglnasplysgly

65707580

leuglnvalglyglnargvalglyileglytrpthralaargsercys

859095

glyhiscysaspalacysileserglyasnglnileasncysglugln

100105110

glyalavalprothrilemetasnargglyglyphealaglulysleu

115120125

argalaasptrpglntrpvalileproleuprogluasnileaspile

130135140

gluseralaglyproleuleucysglyglyilethrvalphelyspro

145150155160

leuleumethishisilethralathrserargvalglyvalilegly

165170175

ileglyglyleuglyhisilealailelysleuleuhisalametgly

180185190

cysgluvalthralapheserserasnproalalysgluglngluval

195200205

leualametglyalaasplysvalvalasnserargaspproglnala

210215220

leulysalaleualaglyglnpheaspleuileileasnthrvalasn

225230235240

valserleuasptrpglnprotyrpheglualaleuthrtyrglygly

245250255

asnphehisthrvalglyalavalleuthrproleuservalproala

260265270

phethrleuilealaglyaspargservalserglyseralathrgly

275280285

thrprotyrgluleuarglysleumetargphealaalaargserlys

290295300

valalaprothrthrgluleupheprometserlysileasnaspala

305310315320

ileglnhisvalargaspglylysalaargtyrargvalvalleulys

325330335

alaaspphe

<210>15

<211>1053

<212>dna

<213>unkown

<400>15

atgaattatcagaacgacgatttacgcatcaaagaaatcaaagagttacttcctcctgtc60

gcattgctggaaaaattccccgctactgaaaatgccgcgaatacggttgcccatgcccga120

aaagcgatccataagatcctgaaaggtaatgatgatcgcctgttggttgtgattggccca180

tgctcaattcatgatcctgtcgcggcaaaagagtatgccactcgcttgctggcgctgcgt240

gaagagctgaaagatgagctggaaatcgtaatgcgcgtctattttgaaaagccgcgtacc300

acggtgggctggaaagggctgattaacgatccgcatatggataatagcttccagatcaac360

gacggtctgcgtatagcccgtaaattgctgcttgatattaacgacagcggtctgccagcg420

gcaggtgagtttctcaatatgatcaccccacaatatctcgctgacctgatgagctggggc480

gcaattggcgcacgtaccaccgaatcgcaggtgcaccgcgaactggcatcagggctttct540

tgtccggtcggcttcaaaaatggcaccgacggtacgattaaagtggctatcgatgccatt600

aatgccgccggtgcgccgcactgcttcctgtccgtaacgaaatgggggcattcggcgatt660

gtgaataccagcggtaacggcgattgccatatcattctgcgcggcggtaaagagcctaac720

tacagcgcgaagcacgttgctgaagtgaaagaagggctgaacaaagcaggcctgccagca780

caggtgatgatcgatttcagccatgctaactcgtccaaacaattcaaaaagcagatggat840

gtttgtgctgacgtttgccagcagattgccggtggcgaaaaggccattattggcgtgatg900

gtggaaagccatctggtggaaggcaatcagagcctcgagagcggggagccgctggcctac960

ggtaagagcatcaccgatgcctgcatcggctgggaagataccgatgctctgttacgtcaa1020

ctggcgaatgcagtaaaagcgcgtcgcgggtaa1053

<210>16

<211>350

<212>prt

<213>unkown

<400>16

metasntyrglnasnaspaspleuargilelysgluilelysgluleu

151015

leuproprovalalaleuleuglulyspheproalathrgluasnala

202530

alaasnthrvalalahisalaarglysalailehislysileleulys

354045

glyasnaspaspargleuleuvalvalileglyprocysserilehis

505560

aspprovalalaalalysglutyralathrargleuleualaleuarg

65707580

glugluleulysaspgluleugluilevalmetargvaltyrpheglu

859095

lysproargthrthrvalglytrplysglyleuileasnaspprohis

100105110

metaspasnserpheglnileasnaspglyleuargilealaarglys

115120125

leuleuleuaspileasnaspserglyleuproalaalaglygluphe

130135140

leuasnmetilethrproglntyrleualaaspleumetsertrpgly

145150155160

alaileglyalaargthrthrgluserglnvalhisarggluleuala

165170175

serglyleusercysprovalglyphelysasnglythraspglythr

180185190

ilelysvalalaileaspalaileasnalaalaglyalaprohiscys

195200205

pheleuservalthrlystrpglyhisseralailevalasnthrser

210215220

glyasnglyaspcyshisileileleuargglyglylysgluproasn

225230235240

tyrseralalyshisvalalagluvallysgluglyleuasnlysala

245250255

glyleuproalaglnvalmetileasppheserhisalaasnserser

260265270

lysglnphelyslysglnmetaspvalcysalaaspvalcysglngln

275280285

ilealaglyglyglulysalaileileglyvalmetvalgluserhis

290295300

leuvalgluglyasnglnserleugluserglygluproleualatyr

305310315320

glylysserilethraspalacysileglytrpgluaspthraspala

325330335

leuleuargglnleualaasnalavallysalaargarggly

340345350

<210>17

<211>1122

<212>dna

<213>unkown

<400>17

atggttgctgaattgaccgcattacgcgatcaaattgatgaagtcgataaagcgctgctg60

aatttattagcgaagcgtctggaactggttgctgaagtgggcgaggtgaaaagccgcttt120

ggactgcctatttatgttccggagcgcgaggcatctattttggcctcgcggcgcgcagag180

gcggaagctctgggtgtaccgccagatctgattgaggatgttttgcgtcgggtgatgcgt240

gaatcttactccagtgaaaacgacaaaggatttaaaacactttgtccgtcactgcgtccg300

gtggttatcgtcggcggtggcggtcagatgggacgcctgttcgagaagatgctgacacta360

tcgggttatcaggtgcggattctggagcaacatgactgggatcgagcggctgatattgtt420

gccgatgccggaatggtgattgttagtgtgccaatccacgttactgagcaagttatcggc480

aaattaccgcctttaccgaaagattgtattctggttgatctggcatcagtgaaaaatgga540

ccattacaggccatgctggcggcgcacgatggcccggtactggggttacacccgatgttc600

ggcccggacagcggtagcctggcaaagcaagttgtggtctggtgtgatggacgtaagccg660

gaagcataccaatggtttctggagcaaattcaggtctggggcgctcggctgcatcgtatt720

agcgctgtcgagcacgatcagaatatggcgtttattcaggctctgcgccactttgctact780

tttgcttatgggctgcatctggcagaagaaaatgttcagcttgagcaacttctggcgctc840

tcttcgccgatttaccgccttgagctggcgatggtcgggcgactgtttgctcaggatccg900

cagctttatgccgacattattatgtcgtcagagcgtaatctggcgttaatcaaacgttac960

tataagcgtttcggcgaggcgattgagttgctggagcagggcgataagcaggcgtttatt1020

gacagtttccgcaaggtggagcactggttcggcgattacgttcagcgttttcagagtgaa1080

agccgcgtgttattgcgtcaggcgaatgacaatcgccagtaa1122

<210>18

<211>373

<212>prt

<213>unkown

<400>18

metvalalagluleuthralaleuargaspglnileaspgluvalasp

151015

lysalaleuleuasnleuleualalysargleugluleuvalalaglu

202530

valglygluvallysserargpheglyleuproiletyrvalproglu

354045

argglualaserileleualaserargargalaglualaglualaleu

505560

glyvalproproaspleuilegluaspvalleuargargvalmetarg

65707580

glusertyrsersergluasnasplysglyphelysthrleucyspro

859095

serleuargprovalvalilevalglyglyglyglyglnmetglyarg

100105110

leupheglulysmetleuthrleuserglytyrglnvalargileleu

115120125

gluglnhisasptrpaspargalaalaaspilevalalaaspalagly

130135140

metvalilevalservalproilehisvalthrgluglnvalilegly

145150155160

lysleuproproleuprolysaspcysileleuvalaspleualaser

165170175

vallysasnglyproleuglnalametleualaalahisaspglypro

180185190

valleuglyleuhisprometpheglyproaspserglyserleuala

195200205

lysglnvalvalvaltrpcysaspglyarglysproglualatyrgln

210215220

trppheleugluglnileglnvaltrpglyalaargleuhisargile

225230235240

seralavalgluhisaspglnasnmetalapheileglnalaleuarg

245250255

hisphealathrphealatyrglyleuhisleualaglugluasnval

260265270

glnleugluglnleuleualaleuserserproiletyrargleuglu

275280285

leualametvalglyargleuphealaglnaspproglnleutyrala

290295300

aspileilemetsersergluargasnleualaleuilelysargtyr

305310315320

tyrlysargpheglyglualailegluleuleugluglnglyasplys

325330335

glnalapheileaspserphearglysvalgluhistrppheglyasp

340345350

tyrvalglnargpheglnsergluserargvalleuleuargglnala

355360365

asnaspasnarggln

370

<210>19

<211>1908

<212>dna

<213>unkown

<400>19

atggcacctgttacaattgaaaagttcgtaaatcaagaagaacgacaccttgtttccaac60

cgatcagcaacaattccgtttggtgaatacatatttaaaagattgttgtccatcgatacg120

aaatcagttttcggtgttcctggtgacttcaacttatctctattagaatatctctattca180

cctagtgttgaatcagctggcctaagatgggtcggcacgtgtaatgaactgaacgccgct240

tatgcggccgacggatattcccgttactctaataagattggctgtttaataaccacgtat300

ggcgttggtgaattaagcgccttgaacggtatagccggttcgttcgctgaaaatgtcaaa360

gttttgcacattgttggtgtggccaagtccatagattcgcgttcaagtaactttagtgat420

cggaacctacatcatttggtcccacagctacatgattcaaattttaaagggccaaatcat480

aaagtatatcatgatatggtaaaagatagagtcgcttgctcggtagcctacttggaggat540

attgaaactgcatgtgaccaagtcgataatgttatccgcgatatttacaagtattctaaa600

cctggttatatttttgttcctgcagattttgcggatatgtctgttacatgtgataatttg660

gttaatgttccacgtatatctcaacaagattgtatagtatacccttctgaaaaccaattg720

tctgacataatcaacaagattactagttggatatattccagtaaaacacctgcgatcctt780

ggagacgtactgactgataggtatggtgtgagtaactttttgaacaagcttatctgcaaa840

actgggatttggaatttttccactgttatgggaaaatctgtaattgatgagtcaaaccca900

acttatatgggtcaatataatggtaaagaaggtttaaaacaagtctatgaacattttgaa960

ctgtgcgacttggtcttgcattttggagtcgacatcaatgaaattaataatgggcattat1020

acttttacttataaaccaaatgctaaaatcattcaatttcatccgaattatattcgcctt1080

gtggacactaggcagggcaatgagcaaatgttcaaaggaatcaattttgcccctatttta1140

aaagaactatacaagcgcattgacgtttctaaactttctttgcaatatgattcaaatgta1200

actcaatatacgaacgaaacaatgcggttagaagatcctaccaatggacaatcaagcatt1260

attacacaagttcacttacaaaagacgatgcctaaatttttgaaccctggtgatgttgtc1320

gtttgtgaaacaggctcttttcaattctctgttcgtgatttcgcgtttccttcgcaatta1380

aaatatatatcgcaaggatttttcctttccattggcatggcccttcctgccgccctaggt1440

gttggaattgccatgcaagaccactcaaacgctcacatcaatggtggcaacgtaaaagag1500

gactataagccaagattaattttgtttgaaggtgacggtgcagcacagatgacaatccaa1560

gaactgagcaccattctgaagtgcaatattccactagaagttatcatttggaacaataac1620

ggctacactattgaaagagccatcatgggccctaccaggtcgtataacgacgttatgtct1680

tggaaatggaccaaactatttgaagcattcggagacttcgacggaaagtatactaatagc1740

actctcattcaatgtccctctaaattagcactgaaattggaggagcttaagaattcaaac1800

aaaagaagcgggatagaacttttagaagtcaaattaggcgaattggatttccccgaacag1860

ctaaagtgcatggttgaagcagcggcacttaaaagaaataaaaaatag1908

<210>20

<211>635

<212>prt

<213>unkown

<400>20

metalaprovalthrileglulysphevalasnglnglugluarghis

151015

leuvalserasnargseralathrilepropheglyglutyrilephe

202530

lysargleuleuserileaspthrlysservalpheglyvalprogly

354045

asppheasnleuserleuleuglutyrleutyrserproservalglu

505560

seralaglyleuargtrpvalglythrcysasngluleuasnalaala

65707580

tyralaalaaspglytyrserargtyrserasnlysileglycysleu

859095

ilethrthrtyrglyvalglygluleuseralaleuasnglyileala

100105110

glyserphealagluasnvallysvalleuhisilevalglyvalala

115120125

lysserileaspserargserserasnpheseraspargasnleuhis

130135140

hisleuvalproglnleuhisaspserasnphelysglyproasnhis

145150155160

lysvaltyrhisaspmetvallysaspargvalalacysservalala

165170175

tyrleugluaspilegluthralacysaspglnvalaspasnvalile

180185190

argaspiletyrlystyrserlysproglytyrilephevalproala

195200205

aspphealaaspmetservalthrcysaspasnleuvalasnvalpro

210215220

argileserglnglnaspcysilevaltyrprosergluasnglnleu

225230235240

seraspileileasnlysilethrsertrpiletyrserserlysthr

245250255

proalaileleuglyaspvalleuthraspargtyrglyvalserasn

260265270

pheleuasnlysleuilecyslysthrglyiletrpasnpheserthr

275280285

valmetglylysservalileaspgluserasnprothrtyrmetgly

290295300

glntyrasnglylysgluglyleulysglnvaltyrgluhispheglu

305310315320

leucysaspleuvalleuhispheglyvalaspileasngluileasn

325330335

asnglyhistyrthrphethrtyrlysproasnalalysileilegln

340345350

phehisproasntyrileargleuvalaspthrargglnglyasnglu

355360365

glnmetphelysglyileasnphealaproileleulysgluleutyr

370375380

lysargileaspvalserlysleuserleuglntyraspserasnval

385390395400

thrglntyrthrasngluthrmetargleugluaspprothrasngly

405410415

glnserserileilethrglnvalhisleuglnlysthrmetprolys

420425430

pheleuasnproglyaspvalvalvalcysgluthrglyserphegln

435440445

pheservalargaspphealapheproserglnleulystyrileser

450455460

glnglyphepheleuserileglymetalaleuproalaalaleugly

465470475480

valglyilealametglnasphisserasnalahisileasnglygly

485490495

asnvallysgluasptyrlysproargleuileleuphegluglyasp

500505510

glyalaalaglnmetthrileglngluleuserthrileleulyscys

515520525

asnileproleugluvalileiletrpasnasnasnglytyrthrile

530535540

gluargalailemetglyprothrargsertyrasnaspvalmetser

545550555560

trplystrpthrlysleupheglualapheglyasppheaspglylys

565570575

tyrthrasnserthrleuileglncysproserlysleualaleulys

580585590

leuglugluleulysasnserasnlysargserglyilegluleuleu

595600605

gluvallysleuglygluleuasppheprogluglnleulyscysmet

610615620

valglualaalaalaleulysargasnlyslys

625630635

<210>21

<211>1419

<212>dna

<213>unkown

<400>21

atgacccccgaacagttccgtcagtatggccaccagctgatcgatttaatcgcagattat60

cgccagaccgttggtgaacgtccggttatggcacaagttgaaccgggctatctgaaagca120

gctttaccggcaacagcacctcaacaaggtgaacctttcgccgccattttagatgatgtg180

aacaatctggtgatgccgggtctgagccactggcagcatccggacttctatggctacttt240

ccgagcaatggtactttaagcagtgtgctgggcgactttctgagtaccggtctgggtgtt300

ctgggtttaagttggcagagcagcccggcactgagcgaactggaggaaacaacactggac360

tggctgcgtcagctgctgggtctgagcggtcagtggagcggtgtgatccaagatacagcc420

agcaccagtactttagtggctttaattagcgcacgtgaacgcgccaccgattacgcactg480

gttcgtggtggtttacaagctgaaccgaaaccgctgatcgtgtacgtgagcgcacatgcc540

catagcagcgtggataaagccgcactgctggccggttttggccgcgataatattcgttta600

attccgaccgacgaacgttacgcactgcgtccggaagctttacaagctgcaattgagcaa660

gatctggccgccggcaatcagccgtgcgcagttgttgcaaccaccggtaccaccaccaca720

accgcattagatccgctgcgtcccgttggcgaaatcgcacaagctaatggtttatggctg780

cacgtggacagcgcaatggctggtagcgccatgattctgccggaatgccgttggatgtgg840

gacggtatcgagttagcagacagcgttgtggtgaacgcacacaaatggctgggtgtggcc900

ttcgactgcagcatctattacgtgcgcgatccgcagcacttaatccgcgtgatgagcacc960

aatccgagctatctgcagagcgcagtggacggtgaggtgaaaaatttacgtgattggggc1020

attccgctgggccgtcgtttccgtgctttaaaactgtggttcatgctgcgcagtgaaggc1080

gtggatgcactgcaagctcgtctgcgtcgtgatctggataatgcacagtggctggctggt1140

caagttgaagcagcagcagaatgggaggtgctggccccggttcaactgcagactttatgc1200

attcgccaccgtcccgctggtctggaaggcgaggcactggatgcccataccaaaggttgg1260

gccgaacgtttaaatgcaagcggtgccgcatacgtgaccccggcaactttagatggtcgc1320

tggatggtgcgtgttagcattggtgctttaccgaccgaacgtggtgatgttcagcgtctg1380

tgggcacgtttacaagacgtgattaaaggcggcagctaa1419

<210>22

<211>472

<212>prt

<213>unkown

<400>22

metthrprogluglnpheargglntyrglyhisglnleuileaspleu

151015

ilealaasptyrargglnthrvalglygluargprovalmetalagln

202530

valgluproglytyrleulysalaalaleuproalathralaprogln

354045

glnglygluprophealaalaileleuaspaspvalasnasnleuval

505560

metproglyleuserhistrpglnhisproaspphetyrglytyrphe

65707580

proserasnglythrleuserservalleuglyasppheleuserthr

859095

glyleuglyvalleuglyleusertrpglnserserproalaleuser

100105110

gluleuglugluthrthrleuasptrpleuargglnleuleuglyleu

115120125

serglyglntrpserglyvalileglnaspthralaserthrserthr

130135140

leuvalalaleuileseralaarggluargalathrasptyralaleu

145150155160

valargglyglyleuglnalagluprolysproleuilevaltyrval

165170175

seralahisalahisserservalasplysalaalaleuleualagly

180185190

pheglyargaspasnileargleuileprothraspgluargtyrala

195200205

leuargproglualaleuglnalaalailegluglnaspleualaala

210215220

glyasnglnprocysalavalvalalathrthrglythrthrthrthr

225230235240

thralaleuaspproleuargprovalglygluilealaglnalaasn

245250255

glyleutrpleuhisvalaspseralametalaglyseralametile

260265270

leuproglucysargtrpmettrpaspglyilegluleualaaspser

275280285

valvalvalasnalahislystrpleuglyvalalapheaspcysser

290295300

iletyrtyrvalargaspproglnhisleuileargvalmetserthr

305310315320

asnprosertyrleuglnseralavalaspglygluvallysasnleu

325330335

argasptrpglyileproleuglyargargpheargalaleulysleu

340345350

trpphemetleuargsergluglyvalaspalaleuglnalaargleu

355360365

argargaspleuaspasnalaglntrpleualaglyglnvalgluala

370375380

alaalaglutrpgluvalleualaprovalglnleuglnthrleucys

385390395400

ilearghisargproalaglyleugluglyglualaleuaspalahis

405410415

thrlysglytrpalagluargleuasnalaserglyalaalatyrval

420425430

thrproalathrleuaspglyargtrpmetvalargvalserilegly

435440445

alaleuprothrgluargglyaspvalglnargleutrpalaargleu

450455460

glnaspvalilelysglyglyser

465470

<210>23

<211>609

<212>dna

<213>unkown

<400>23

atgcgtaaagtgatcaaatatgatatggaggtggccgtgagcgccgatagcgtttgggca60

gtgtatagtagcccggatatcccgcgtctgctgcgcgatgttctgctgccgggtgtgttc120

gagaagctggacgtgatcgaaggcaatggtggtgttggcaccgttctggacattgtgttt180

ccgccgggcgcagttccgcgcagctataaagaaaaatttgtgaacattgatcgcgaaaag240

cgtttaaaagaagttatcatgatcgaaggcggttatttagatatgggctgcaccttctat300

ctggatcgcattcacgtggtggagaaaaccaagagcagctgcgtgatcgagagcagcatt360

gtgtacgacgtgaaagaggagtgtgccgatgccatgagcaagctgattaccaccgaaccg420

ctgaaaagcatggccgaagtgattagcaattatgtgattcagaaagagctgttcagcgcc480

cgcaacattttaagcaagcagagcgtggtgaaaaaggagatccgctatgatttagaggtg540

ccgattagcgtggacagcatttggagcgtgtacagctgcccggatattcctcgtttactg600

ggcagctaa609

<210>24

<211>202

<212>prt

<213>unkown

<400>24

metarglysvalilelystyraspmetgluvalalavalseralaasp

151015

servaltrpalavaltyrserserproaspileproargleuleuarg

202530

aspvalleuleuproglyvalpheglulysleuaspvalileglugly

354045

asnglyglyvalglythrvalleuaspilevalpheproproglyala

505560

valproargsertyrlysglulysphevalasnileaspargglulys

65707580

argleulysgluvalilemetilegluglyglytyrleuaspmetgly

859095

cysthrphetyrleuaspargilehisvalvalglulysthrlysser

100105110

sercysvalilegluserserilevaltyraspvallysgluglucys

115120125

alaaspalametserlysleuilethrthrgluproleulyssermet

130135140

alagluvalileserasntyrvalileglnlysgluleupheserala

145150155160

argasnileleuserlysglnservalvallyslysgluileargtyr

165170175

aspleugluvalproileservalaspseriletrpservaltyrser

180185190

cysproaspileproargleuleuglyser

195200

<210>25

<211>1041

<212>dna

<213>unkown

<400>25

atggagaccgttagcaaaattgaccaacagaatcaagctaaaatctggaagcagatctat60

ggcttcgccgaatctttagttctgaaatgtgccgtgcagctggaaatcgccgagacactg120

cacaacaacgtgaaaccgatgtctttaagcgaactggccagcaagctgccggttgcacag180

ccggttaacgaagaccgtttattccgcatcatgcgctatttagtgcatatggaactgttc240

aaaattgatgccaccacccagaaatactctttagccccgccggccaaatatttactgcgc300

ggctgggagaaaagcatggtggatagcattctgtgcatcaacgataaggactttctggcc360

ccttggcaccatctgggtgatggtctgaccggtaattgcgatgcattcgagaaagcttta420

ggcaagagcatttgggtgtatatgagcgaaaacccggagaaaaaccagctgttcaacgcc480

gcaatggcatgcgatacccgtttagtgacaagcgcactggccaacgagtgcaagagcatc540

tttagcgacggcattagcactttagttgatgttggcggcggtaccggtaccgcagtgaag600

gcaatcagcaaagcattcccggacattaaatgcaccatttatgatttaccgcatgtgatc660

gccgatagcccggagattccgaacatcaccaagatcagtggcgacatgttcaaaagtatc720

ccgagcgccgacgccatctttatgaagtgcattttacacgactggaatgacgacgaatgc780

attcagattttaaaacgctgcaaagaagctttaccgaaagttggcaaggtgatcatcgtg840

gacgtggttatcgacatggacagcacccatccgtatgccaaaatccgtttaactttagat900

ctggacatgatgctgaacaccggcggcaaagaacgcaccaaagaagaatggaaaacttta960

ttcgatgcagccggttttgccagccacaaagtgacacagattagcgccgtgcagagcgtg1020

attgaagcatatccgtactaa1041

<210>26

<211>346

<212>prt

<213>unkown

<400>26

metgluthrvalserlysileaspglnglnasnglnalalysiletrp

151015

lysglniletyrglyphealagluserleuvalleulyscysalaval

202530

glnleugluilealagluthrleuhisasnasnvallysprometser

354045

leusergluleualaserlysleuprovalalaglnprovalasnglu

505560

aspargleupheargilemetargtyrleuvalhismetgluleuphe

65707580

lysileaspalathrthrglnlystyrserleualaproproalalys

859095

tyrleuleuargglytrpglulyssermetvalaspserileleucys

100105110

ileasnasplysasppheleualaprotrphishisleuglyaspgly

115120125

leuthrglyasncysaspalapheglulysalaleuglylysserile

130135140

trpvaltyrmetsergluasnproglulysasnglnleupheasnala

145150155160

alametalacysaspthrargleuvalthrseralaleualaasnglu

165170175

cyslysserilepheseraspglyileserthrleuvalaspvalgly

180185190

glyglythrglythralavallysalaileserlysalapheproasp

195200205

ilelyscysthriletyraspleuprohisvalilealaaspserpro

210215220

gluileproasnilethrlysileserglyaspmetphelysserile

225230235240

proseralaaspalailephemetlyscysileleuhisasptrpasn

245250255

aspaspglucysileglnileleulysargcyslysglualaleupro

260265270

lysvalglylysvalileilevalaspvalvalileaspmetaspser

275280285

thrhisprotyralalysileargleuthrleuaspleuaspmetmet

290295300

leuasnthrglyglylysgluargthrlysgluglutrplysthrleu

305310315320

pheaspalaalaglyphealaserhislysvalthrglnileserala

325330335

valglnservalileglualatyrprotyr

340345

<210>27

<211>1053

<212>dna

<213>unkown

<400>27

atggccttccatggcaaggacgacgtgctggatattaaggcccaagctcatgtgtggaag60

atcatttacggcttcgctgattctttagtgctgcgttgcgccgtggagttaggcatcgtg120

gatatcattgataacaataaccaaccgatggctttagccgatctggccagcaaactgccg180

gttagcgacgtgaactgcgataatctgtaccgcattctgcgctatttagttaagatggag240

attttacgcgtggagaaaagcgatgacggccagaaaaagtatgctttagaaccgattgcc300

actttactgagtcgcaatgccaaacgtagcatggtgccgatgattttaggcatgacccag360

aaggatttcatgaccccgtggcacagcatgaaagatggtttaagcgataatggcaccgcc420

tttgagaaggcaatgggcatgaccatctgggaatatttagaaggtcacccggatcagagc480

cagctgttcaatgagggcatggctggtgaaacccgtttactgaccagcagtctgatcagc540

ggtagccgcgacatgtttcaaggtatcgattctctggtggatgttggtggtggcaatggt600

accaccgttaaggccattagcgacgccttcccgcacatcaaatgcacactgttcgattta660

cctcacgtgatcgccaacagctacgatctgccgaacattgaacgcatcggtggcgatatg720

tttaaaagcgtgccgagcgcccaagctatcattttaaagctgattttacatgactggaac780

gatgaggacagcatcaagattttaaagcagtgccgcaacgccgtgccgaaagatggcggc840

aaagtgatcatcgtggatgtggctttagacgaggaaagcgatcatgagctgagtagcacc900

cgtctgattttagacatcgatatgctggtgaataccggcggtaaagagcgtaccaaggaa960

gtttgggaaaaaatcgtgaagagcgccggctttagcggctgcaaaattcgccacattgcc1020

gccatccagagcgtgattgaggtgtttccttaa1053

<210>28

<211>350

<212>prt

<213>unkown

<400>28

metalaphehisglylysaspaspvalleuaspilelysalaglnala

151015

hisvaltrplysileiletyrglyphealaaspserleuvalleuarg

202530

cysalavalgluleuglyilevalaspileileaspasnasnasngln

354045

prometalaleualaaspleualaserlysleuprovalseraspval

505560

asncysaspasnleutyrargileleuargtyrleuvallysmetglu

65707580

ileleuargvalglulysseraspaspglyglnlyslystyralaleu

859095

gluproilealathrleuleuserargasnalalysargsermetval

100105110

prometileleuglymetthrglnlysaspphemetthrprotrphis

115120125

sermetlysaspglyleuseraspasnglythralapheglulysala

130135140

metglymetthriletrpglutyrleugluglyhisproaspglnser

145150155160

glnleupheasngluglymetalaglygluthrargleuleuthrser

165170175

serleuileserglyserargaspmetpheglnglyileaspserleu

180185190

valaspvalglyglyglyasnglythrthrvallysalaileserasp

195200205

alapheprohisilelyscysthrleupheaspleuprohisvalile

210215220

alaasnsertyraspleuproasnilegluargileglyglyaspmet

225230235240

phelysservalproseralaglnalaileileleulysleuileleu

245250255

hisasptrpasnaspgluaspserilelysileleulysglncysarg

260265270

asnalavalprolysaspglyglylysvalileilevalaspvalala

275280285

leuaspglugluserasphisgluleuserserthrargleuileleu

290295300

aspileaspmetleuvalasnthrglyglylysgluargthrlysglu

305310315320

valtrpglulysilevallysseralaglypheserglycyslysile

325330335

arghisilealaalaileglnservalilegluvalphepro

340345350

<210>29

<211>1056

<212>dna

<213>unkown

<400>29

atgcagttaaaagaaaaagaggagctgctgcgcaacatggaactgggtctgatcccggat60

caagaaattcgccagcgtatccgcatcgagctggaaaaacgtttacagtggggctacaaa120

gagacccatgaggaacagctgagtcagctgctggatttagtgcactctttaaagggcatg180

aaaatggccaccgaaatggagaatctggatttaaagctgtatgaagccccgatggagttt240

ttaaagatccagcacggtagcaacatgaagcagagcgctggttactataccgacgaaagc300

accactttagatgaagccgagattgccatgctggatctgtacatggaacgcgcacagatc360

aaggatggccagagcgttttagatttaggctgcggcttaggcgccgttgcactgtttggc420

gccaataaatttaagaagtgccagtttaccggtgttaccagcagtgtggagcagaaagac480

tatattgaaggcaaatgcaaagaactgaaactgaccaacgtgaaagtgctgctggcagac540

atcaccacctatgaaaccgaggagcgctttgatcgcatctttgccgtggagctgatcgag600

catatgaaaaactatcagctgttactgaagaaaattagcgagtggatgaaggatgatggt660

ttactgtttgtggaacacgtgtgccacaagactttagcctatcattacgaaccggtggac720

gcagaggattggtacaccaactatattttcccggccggcacactgactttaagcagtgcc780

agcatgctgctgtatttccaagatgacgttagcgtggttaaccagtggactttaagtggc840

aagcactattctcgcagccacgaagaatggctgaaaaacatggataagaatattgtggaa900

tttaaggaaattatgcgtagtatcaccaaaaccgaaaaagaagccattaaactgttaaac960

ttctggcgtattttctgcatgtgcggcgccgagctgtttggctataaaaacggcgaggag1020

tggatgttaacccatctgctgtttaagaaaaaataa1056

<210>30

<211>351

<212>prt

<213>unkown

<400>30

metglnleulysglulysglugluleuleuargasnmetgluleugly

151015

leuileproaspglngluileargglnargileargilegluleuglu

202530

lysargleuglntrpglytyrlysgluthrhisglugluglnleuser

354045

glnleuleuaspleuvalhisserleulysglymetlysmetalathr

505560

glumetgluasnleuaspleulysleutyrglualaprometgluphe

65707580

leulysileglnhisglyserasnmetlysglnseralaglytyrtyr

859095

thraspgluserthrthrleuaspglualagluilealametleuasp

100105110

leutyrmetgluargalaglnilelysaspglyglnservalleuasp

115120125

leuglycysglyleuglyalavalalaleupheglyalaasnlysphe

130135140

lyslyscysglnphethrglyvalthrserservalgluglnlysasp

145150155160

tyrilegluglylyscyslysgluleulysleuthrasnvallysval

165170175

leuleualaaspilethrthrtyrgluthrglugluargpheasparg

180185190

ilephealavalgluleuilegluhismetlysasntyrglnleuleu

195200205

leulyslysileserglutrpmetlysaspaspglyleuleupheval

210215220

gluhisvalcyshislysthrleualatyrhistyrgluprovalasp

225230235240

alagluasptrptyrthrasntyrilepheproalaglythrleuthr

245250255

leuserseralasermetleuleutyrpheglnaspaspvalserval

260265270

valasnglntrpthrleuserglylyshistyrserargserhisglu

275280285

glutrpleulysasnmetasplysasnilevalgluphelysgluile

290295300

metargserilethrlysthrglulysglualailelysleuleuasn

305310315320

phetrpargilephecysmetcysglyalagluleupheglytyrlys

325330335

asnglygluglutrpmetleuthrhisleuleuphelyslyslys

340345350

<210>31

<211>1563

<212>dna

<213>unkown

<400>31

atgaaaccagaagatttccgcgccagtacccaacgtcctttcaccggggaagagtatctg60

aaaagcctgcaggatggtcgcgagatctatatctatggcgagcgagtgaaagacgtcacc120

actcatccggcatttcgtaatgcggcagcgtctgttgcccagctgtacgacgcactgcac180

aaaccggagatgcaggactctctgtgttggaacaccgacaccggcagcggcggctatacc240

cataaattcttccgcgtggcgaaaagtgccgacgacctgcgccagcaacgcgacgccatc300

gctgagtggtcacgcctgagctatggctggatgggccgtaccccagactacaaagccgct360

ttcggttgcgcactgggcgcgaatccgggcttttacggtcagttcgagcagaacgcccgt420

aactggtacacccgtattcaggaaactggcctctactttaaccacgcgattgttaaccca480

ccgatcgatcgtcatttgccgaccgataaagtgaaagacgtttacatcaagctggaaaaa540

gagactgacgccgggattatcgtcagcggtgcgaaagtggttgccaccaactcggcgctg600

actcactacaacatgattggcttcggctcggcacaagtgatgggcgaaaacccggacttc660

gcactgatgttcgttgcgccaatggatgccgatggcgtgaaattaatctcccgcgcctct720

tatgagatggtcgcgggtgctaccggctcgccatacgactacccgctctccagccgcttc780

gatgagaacgatgcgattctggtgatggataacgtgctgattccatgggaaaacgtgctg840

atctaccgcgattttgatcgctgccgtcgctggacgatggaaggcggttttgcccgtatg900

tatccgctgcaagcctgtgtgcgcctggcagtgaaattagacttcattacggcactgctg960

aaaaaatcactcgaatgtaccggcaccctggagttccgtggtgtgcaggccgatctcggt1020

gaagtggtagcgtggcgcaacaccttctgggcattgagtgactcgatgtgttcagaagca1080

acgccgtgggtcaacggggcttatttaccggatcatgccgcactgcaaacctatcgcgta1140

ctggcaccaatggcctacgcgaagatcaaaaacattatcgaacgcaacgttaccagtggc1200

ctgatctatctcccttccagtgcccgtgacctgaataatccgcagatcgaccagtatctg1260

gcgaagtatgtgcgcggttcgaacggtatggatcacgtccagcgcatcaagatcctcaaa1320

ctgatgtgggatgctattggcagcgaatttggtggtcgtcacgaactgtatgaaatcaac1380

tactccggtagccaggatgagattcgcctgcagtgtctgcgccaggcacaaaactccggc1440

aatatggacaagatgatggcgatggttgatcgctgcctgtcggaatacgaccaggacggc1500

tggactgtgccgcacctgcacaacaacgacgatatcaacatgctggataagctgctgaaa1560

taa1563

<210>32

<211>520

<212>prt

<213>unkown

<400>32

metlysprogluasppheargalaserthrglnargprophethrgly

151015

gluglutyrleulysserleuglnaspglyarggluiletyriletyr

202530

glygluargvallysaspvalthrthrhisproalapheargasnala

354045

alaalaservalalaglnleutyraspalaleuhislysproglumet

505560

glnaspserleucystrpasnthraspthrglyserglyglytyrthr

65707580

hislysphepheargvalalalysseralaaspaspleuargglngln

859095

argaspalailealaglutrpserargleusertyrglytrpmetgly

100105110

argthrproasptyrlysalaalapheglycysalaleuglyalaasn

115120125

proglyphetyrglyglnphegluglnasnalaargasntrptyrthr

130135140

argileglngluthrglyleutyrpheasnhisalailevalasnpro

145150155160

proileasparghisleuprothrasplysvallysaspvaltyrile

165170175

lysleuglulysgluthraspalaglyileilevalserglyalalys

180185190

valvalalathrasnseralaleuthrhistyrasnmetileglyphe

195200205

glyseralaglnvalmetglygluasnproaspphealaleumetphe

210215220

valalaprometaspalaaspglyvallysleuileserargalaser

225230235240

tyrglumetvalalaglyalathrglyserprotyrasptyrproleu

245250255

serserargpheaspgluasnaspalaileleuvalmetaspasnval

260265270

leuileprotrpgluasnvalleuiletyrargasppheaspargcys

275280285

argargtrpthrmetgluglyglyphealaargmettyrproleugln

290295300

alacysvalargleualavallysleuasppheilethralaleuleu

305310315320

lyslysserleuglucysthrglythrleuglupheargglyvalgln

325330335

alaaspleuglygluvalvalalatrpargasnthrphetrpalaleu

340345350

seraspsermetcysserglualathrprotrpvalasnglyalatyr

355360365

leuproasphisalaalaleuglnthrtyrargvalleualapromet

370375380

alatyralalysilelysasnileilegluargasnvalthrsergly

385390395400

leuiletyrleuproserseralaargaspleuasnasnproglnile

405410415

aspglntyrleualalystyrvalargglyserasnglymetasphis

420425430

valglnargilelysileleulysleumettrpaspalaileglyser

435440445

glupheglyglyarghisgluleutyrgluileasntyrserglyser

450455460

glnaspgluileargleuglncysleuargglnalaglnasnsergly

465470475480

asnmetasplysmetmetalametvalaspargcysleuserglutyr

485490495

aspglnaspglytrpthrvalprohisleuhisasnasnaspaspile

500505510

asnmetleuasplysleuleulys

515520

<210>33

<211>513

<212>dna

<213>unkown

<400>33

atgcaattagatgaacaacgcctgcgctttcgtgacgcgatggccagcctgtcggcagcg60

gtaaatattatcaccaccgagggcgacgccggacaatgcgggattacggcaacggccgtc120

tgctcggtcacggatacaccaccgtcgctgatggtgtgcattaacgccaacagtgcgatg180

aacccggtttttcagggcaacggcaagttgtgcgtcaacgtcctcaaccatgagcaggaa240

ctgatggcacgccacttcgcgggcatgacaggcatggcgatggaagagcgttttagcctc300

tcatgctggcaaaaaggtccgctggcgcagccggtgctaaaaggttcgctggccagtctt360

gaaggtgagatccgcgatgtgcaggcaattggcacacatctggtgtatctggtggagatt420

aaaaacatcatcctcagtgcagaaggtcatggacttatctactttaaacgccgtttccat480

ccggtgatgctggaaatggaagctgcgatttaa513

<210>34

<211>170

<212>prt

<213>unkown

<400>34

metglnleuaspgluglnargleuargpheargaspalametalaser

151015

leuseralaalavalasnileilethrthrgluglyaspalaglygln

202530

cysglyilethralathralavalcysservalthraspthrpropro

354045

serleumetvalcysileasnalaasnseralametasnprovalphe

505560

glnglyasnglylysleucysvalasnvalleuasnhisgluglnglu

65707580

leumetalaarghisphealaglymetthrglymetalametgluglu

859095

argpheserleusercystrpglnlysglyproleualaglnproval

100105110

leulysglyserleualaserleugluglygluileargaspvalgln

115120125

alaileglythrhisleuvaltyrleuvalgluilelysasnileile

130135140

leuseralagluglyhisglyleuiletyrphelysargargphehis

145150155160

provalmetleuglumetglualaalaile

165170

<210>35

<211>3525

<212>dna

<213>unkown

<400>35

atgagtccgatcacacgcgaggagcgcctggagcgtcgtattcaagacctgtacgccaat60

gatccgcaatttgccgcagccaagccggcaacagcaattaccgccgcaatcgaacgtccg120

ggcctgcctctgcctcagattattgaaaccgttatgacaggctatgccgaccgtcctgca180

ctggcccaacgtagtgtggaatttgtgacagatgcaggcaccggtcacaccaccttacgc240

ctgctgccgcacttcgagaccatcagttacggcgaactgtgggatcgcattagtgccctg300

gccgatgttttaagcaccgagcagaccgtgaaaccgggtgatcgcgtttgcctgctgggt360

ttcaacagcgtggattatgcaacaattgacatgaccctggcccgcctgggtgcagtggcc420

gtgccgctgcaaaccagcgcagcaattacacagctgcagcctattgtggccgaaacccag480

ccgaccatgattgcagccagcgttgacgccttagcagatgccacagagttagcactgagt540

ggtcagaccgcaacacgcgtgctggttttcgaccatcatcgccaggttgatgcacatcgc600

gccgcagttgaaagtgcacgcgaacgcctggccggcagtgcagtggttgagaccttagca660

gaagccattgcacgtggtgacgtgccgcgtggtgcaagtgcaggtagtgccccgggcaca720

gacgttagtgatgacagtctggccctgctgatttacaccagtggcagtacaggcgccccg780

aagggtgcaatgtatcctcgtcgcaatgttgccacattctggcgcaaacgcacctggttt840

gaaggtggttacgaaccgagtatcaccctgaacttcatgccgatgagccatgtgatgggt900

cgccagatcctgtacggcaccctgtgcaatggcggcaccgcctacttcgttgcaaagagc960

gatctgagcacactgtttgaagacctggcattagtgcgcccgaccgaattaaccttcgtg1020

ccgcgtgtttgggacatggttttcgatgaatttcagagcgaagttgaccgccgcctggtt1080

gatggtgcagatcgtgttgccctggaggcccaggttaaagccgagatccgtaatgacgtg1140

ttaggcggccgctatacaagcgcactgaccggcagtgcaccgattagcgatgagatgaag1200

gcctgggttgaagagctgctggacatgcacctggtggaaggttacggcagtacagaagcc1260

ggcatgatcctgattgatggcgcaattcgtcgccctgcagttctggactacaaactggtg1320

gatgtgccggacctgggttacttcttaaccgaccgccctcatccgcgcggtgaactgctg1380

gtgaagaccgacagcttattccctggttattaccagcgcgccgaggttaccgcagacgtt1440

tttgacgccgatggtttctatcgcaccggcgacattatggccgaagtgggccctgaacag1500

ttcgtgtacctggatcgtcgtaataacgttctgaaattaagtcagggcgagtttgttaca1560

gtgagcaaactggaggcagtgttcggtgacagcccgctggtgcgtcagatctatatctat1620

ggcaacagtgcccgcgcatacctgctggcagtgatcgtgcctacccaagaggccctggat1680

gccgttcctgttgaggaattaaaagcccgcctgggcgatagcctgcaggaagtggccaaa1740

gcagccggtctgcaaagttacgaaatcccgcgcgacttcattatcgaaaccaccccgtgg1800

acactggagaacggcctgctgacaggtatccgtaaactggcccgcccgcagctgaagaag1860

cactatggcgagctgctggagcagatctatacagacctggcccacggccaagccgatgag1920

ctgcgtagcctgcgccagagtggtgccgatgcacctgtgctggtgaccgtttgtcgcgca1980

gcagccgccttactgggtggtagcgccagcgatgtgcagcctgacgcccacttcacagac2040

ctgggcggtgatagcttaagtgccctgagttttaccaacctgctgcacgaaatcttcgat2100

atcgaggtgcctgttggtgtgattgttagcccggccaacgatttacaggcactggccgat2160

tatgttgaggcagcacgtaaacctggcagcagtcgcccgacattcgccagcgttcatggt2220

gccagcaatggccaagtgacagaggtgcatgccggcgacctgagtctggataaatttatc2280

gacgccgccaccctggccgaagcacctcgtttaccggcagccaatacccaggttcgcaca2340

gtgctgctgaccggcgccacaggttttctgggccgttatctggcattagagtggctggag2400

cgtatggatctggttgacggcaagctgatctgcctggttcgcgcaaagagtgacacagaa2460

gcccgtgcacgtttagacaagacattcgacagcggcgatccggaactgttagcccactat2520

cgcgcattagcaggcgatcatctggaagtgctggcaggtgataagggcgaagccgatctg2580

ggtctggaccgccagacctggcaacgcttagcagacacagtggacctgatcgttgatcct2640

gcagcactggtgaaccacgtgctgccgtatagtcagctgtttggcccgaatgccctgggt2700

accgcagaactgctgcgcctggccctgaccagtaaaattaagccgtatagttacacaagc2760

accattggcgtggccgaccaaattcctccgagcgcatttaccgaggatgccgatatccgc2820

gttattagtgccacacgtgcagttgacgacagttacgcaaatggttacagcaacagcaag2880

tgggccggcgaggttctgctgcgcgaagcacatgacctgtgcggcctgccggttgccgtg2940

tttcgttgcgatatgatcctggccgataccacctgggcaggtcagttaaacgtgccggat3000

atgttcacccgcatgattttaagcttagccgccaccggtatcgccccgggtagcttttat3060

gagctggcagcagatggcgcccgtcaacgcgcccactatgatggcctgccggtggagttt3120

atcgcagaggccattagcacactgggtgcacagagccaggacggcttccatacctatcac3180

gtgatgaatccgtatgatgacggcatcggtctggacgagttcgttgattggctgaacgaa3240

agcggctgtcctatccagcgcattgccgactacggcgactggctgcaacgtttcgagacc3300

gcactgcgtgccctgcctgatcgtcagcgccacagtagtctgttacctttactgcacaac3360

taccgtcagcctgaacgtccggttcgcggtagcattgcaccgaccgaccgttttcgcgca3420

gccgtgcaagaggcgaagattggcccggataaagatattccgcacgtgggcgccccgatc3480

atcgtgaaatatgtgagtgacctgcgcctgttaggcctgctgtaa3525

<210>36

<211>1174

<212>prt

<213>unkown

<400>36

metserproilethrargglugluargleugluargargileglnasp

151015

leutyralaasnaspproglnphealaalaalalysproalathrala

202530

ilethralaalailegluargproglyleuproleuproglnileile

354045

gluthrvalmetthrglytyralaaspargproalaleualaglnarg

505560

servalgluphevalthraspalaglythrglyhisthrthrleuarg

65707580

leuleuprohisphegluthrilesertyrglygluleutrpasparg

859095

ileseralaleualaaspvalleuserthrgluglnthrvallyspro

100105110

glyaspargvalcysleuleuglypheasnservalasptyralathr

115120125

ileaspmetthrleualaargleuglyalavalalavalproleugln

130135140

thrseralaalailethrglnleuglnproilevalalagluthrgln

145150155160

prothrmetilealaalaservalaspalaleualaaspalathrglu

165170175

leualaleuserglyglnthralathrargvalleuvalpheasphis

180185190

hisargglnvalaspalahisargalaalavalgluseralaargglu

195200205

argleualaglyseralavalvalgluthrleualaglualaileala

210215220

argglyaspvalproargglyalaseralaglyseralaproglythr

225230235240

aspvalseraspaspserleualaleuleuiletyrthrserglyser

245250255

thrglyalaprolysglyalamettyrproargargasnvalalathr

260265270

phetrparglysargthrtrpphegluglyglytyrgluproserile

275280285

thrleuasnphemetprometserhisvalmetglyargglnileleu

290295300

tyrglythrleucysasnglyglythralatyrphevalalalysser

305310315320

aspleuserthrleuphegluaspleualaleuvalargprothrglu

325330335

leuthrphevalproargvaltrpaspmetvalpheaspgluphegln

340345350

sergluvalaspargargleuvalaspglyalaaspargvalalaleu

355360365

glualaglnvallysalagluileargasnaspvalleuglyglyarg

370375380

tyrthrseralaleuthrglyseralaproileseraspglumetlys

385390395400

alatrpvalglugluleuleuaspmethisleuvalgluglytyrgly

405410415

serthrglualaglymetileleuileaspglyalaileargargpro

420425430

alavalleuasptyrlysleuvalaspvalproaspleuglytyrphe

435440445

leuthraspargprohisproargglygluleuleuvallysthrasp

450455460

serleupheproglytyrtyrglnargalagluvalthralaaspval

465470475480

pheaspalaaspglyphetyrargthrglyaspilemetalagluval

485490495

glyprogluglnphevaltyrleuaspargargasnasnvalleulys

500505510

leuserglnglygluphevalthrvalserlysleuglualavalphe

515520525

glyaspserproleuvalargglniletyriletyrglyasnserala

530535540

argalatyrleuleualavalilevalprothrglnglualaleuasp

545550555560

alavalprovalglugluleulysalaargleuglyaspserleugln

565570575

gluvalalalysalaalaglyleuglnsertyrgluileproargasp

580585590

pheileilegluthrthrprotrpthrleugluasnglyleuleuthr

595600605

glyilearglysleualaargproglnleulyslyshistyrglyglu

610615620

leuleugluglniletyrthraspleualahisglyglnalaaspglu

625630635640

leuargserleuargglnserglyalaaspalaprovalleuvalthr

645650655

valcysargalaalaalaalaleuleuglyglyseralaseraspval

660665670

glnproaspalahisphethraspleuglyglyaspserleuserala

675680685

leuserphethrasnleuleuhisgluilepheaspilegluvalpro

690695700

valglyvalilevalserproalaasnaspleuglnalaleualaasp

705710715720

tyrvalglualaalaarglysproglyserserargprothrpheala

725730735

servalhisglyalaserasnglyglnvalthrgluvalhisalagly

740745750

aspleuserleuasplyspheileaspalaalathrleualagluala

755760765

proargleuproalaalaasnthrglnvalargthrvalleuleuthr

770775780

glyalathrglypheleuglyargtyrleualaleuglutrpleuglu

785790795800

argmetaspleuvalaspglylysleuilecysleuvalargalalys

805810815

seraspthrglualaargalaargleuasplysthrpheaspsergly

820825830

aspprogluleuleualahistyrargalaleualaglyasphisleu

835840845

gluvalleualaglyasplysglyglualaaspleuglyleuasparg

850855860

glnthrtrpglnargleualaaspthrvalaspleuilevalasppro

865870875880

alaalaleuvalasnhisvalleuprotyrserglnleupheglypro

885890895

asnalaleuglythralagluleuleuargleualaleuthrserlys

900905910

ilelysprotyrsertyrthrserthrileglyvalalaaspglnile

915920925

proproseralaphethrgluaspalaaspileargvalileserala

930935940

thrargalavalaspaspsertyralaasnglytyrserasnserlys

945950955960

trpalaglygluvalleuleuargglualahisaspleucysglyleu

965970975

provalalavalpheargcysaspmetileleualaaspthrthrtrp

980985990

alaglyglnleuasnvalproaspmetphethrargmetileleuser

99510001005

leualaalathrglyilealaproglyserphetyrgluleualaala

101010151020

aspglyalaargglnargalahistyraspglyleuprovalgluphe

1025103010351040

ilealaglualaileserthrleuglyalaglnserglnaspglyphe

104510501055

histhrtyrhisvalmetasnprotyraspaspglyileglyleuasp

106010651070

gluphevalasptrpleuasngluserglycysproileglnargile

107510801085

alaasptyrglyasptrpleuglnargphegluthralaleuargala

109010951100

leuproaspargglnarghisserserleuleuproleuleuhisasn

1105111011151120

tyrargglnprogluargprovalargglyserilealaprothrasp

112511301135

argpheargalaalavalglnglualalysileglyproasplysasp

114011451150

ileprohisvalglyalaproileilevallystyrvalseraspleu

115511601165

argleuleuglyleuleu

1170

<210>37

<211>675

<212>dna

<213>unkown

<400>37

atgaagatttacggaatttatatggaccgcccgctttcacaggaagaaaatgaacggttc60

atgactttcatatcacctgaaaaacgggagaaatgccggagattttatcataaagaagat120

gctcaccgcaccctgctgggagatgtgctcgttcgctcagtcataagcaggcagtatcag180

ttggacaaatccgatatccgctttagcacgcaggaatacgggaagccgtgcatccctgat240

cttcccgacgctcatttcaacatttctcactccggccgctgggtcattggtgcgtttgat300

tcacagccgatcggcatagatatcgaaaaaacgaaaccgatcagccttgagatcgccaag360

cgcttcttttcaaaaacagagtacagcgaccttttagcaaaagacaaggacgagcagaca420

gactatttttatcatctatggtcaatgaaagaaagctttatcaaacaggaaggcaaaggc480

ttatcgcttccgcttgattccttttcagtgcgcctgcatcaggacggacaagtatccatt540

gagcttccggacagccattccccatgctatatcaaaacgtatgaggtcgatcccggctac600

aaaatggctgtatgcgccgcacaccctgatttccccgaggatatcacaatggtctcgtac660

gaagagcttttataa675

<210>38

<211>224

<212>prt

<213>unkown

<400>38

metlysiletyrglyiletyrmetaspargproleuserglngluglu

151015

asngluargphemetthrpheileserproglulysargglulyscys

202530

argargphetyrhislysgluaspalahisargthrleuleuglyasp

354045

valleuvalargservalileserargglntyrglnleuasplysser

505560

aspileargpheserthrglnglutyrglylysprocysileproasp

65707580

leuproaspalahispheasnileserhisserglyargtrpvalile

859095

glyalapheaspserglnproileglyileaspileglulysthrlys

100105110

proileserleugluilealalysargphepheserlysthrglutyr

115120125

seraspleuleualalysasplysaspgluglnthrasptyrphetyr

130135140

hisleutrpsermetlysgluserpheilelysglngluglylysgly

145150155160

leuserleuproleuaspserpheservalargleuhisglnaspgly

165170175

glnvalserilegluleuproaspserhisserprocystyrilelys

180185190

thrtyrgluvalaspproglytyrlysmetalavalcysalaalahis

195200205

proasppheprogluaspilethrmetvalsertyrglugluleuleu

210215220

<210>39

<211>1488

<212>dna

<213>unkown

<400>39

atgaattttcatcatctggcttactggcaggataaagcgttaagtctcgccattgaaaac60

cgcttatttattaacggtgaatatactgctgcggcggaaaatgaaacctttgaaaccgtt120

gatccggtcacccaggcaccgctggcgaaaattgcccgcggcaagagcgtcgatatcgac180

cgtgcgatgagcgcagcacgcggcgtatttgaacgcggcgactggtcactctcttctccg240

gctaaacgtaaagcggtactgaataaactcgccgatttaatggaagcccacgccgaagag300

ctggcactgctggaaactctcgacaccggcaaaccgattcgtcacagtctgcgtgatgat360

attcccggcgcggcgcgcgccattcgctggtacgccgaagcgatcgacaaagtgtatggc420

gaagtggcgaccaccagtagccatgagctggcgatgatcgtgcgtgaaccggtcggcgtg480

attgccgccatcgtgccgtggaacttcccgctgttgctgacttgctggaaactcggcccg540

gcgctggcggcgggaaacagcgtgattctaaaaccgtctgaaaaatcaccgctcagtgcg600

attcgtctcgcggggctggcgaaagaagcaggcttgccggatggtgtgttgaacgtggtg660

acgggttttggtcatgaagccgggcaggcgctgtcgcgtcataacgatatcgacgccatt720

gcctttaccggttcaacccgtaccgggaaacagctgctgaaagatgcgggcgacagcaac780

atgaaacgcgtctggctggaagcgggcggcaaaagcgccaacatcgttttcgctgactgc840

ccggatttgcaacaggcggcaagcgccaccgcagcaggcattttctacaaccagggacag900

gtgtgcatcgccggaacgcgcctgttgctggaagagagcatcgccgatgaattcttagcc960

ctgttaaaacagcaggcgcaaaactggcagccgggccatccacttgatcccgcaaccacc1020

atgggcaccttaatcgactgcgcccacgccgactcggtccatagctttattcgggaaggc1080

gaaagcaaagggcaactgttgttggatggccgtaacgccgggctggctgccgccatcggc1140

ccgaccatctttgtggatgtggacccgaatgcgtccttaagtcgcgaagagattttcggt1200

ccggtgctggtggtcacgcgtttcacatcagaagaacaggcgctacagcttgccaacgac1260

agccagtacggccttggcgcggcggtatggacgcgcgacctctcccgcgcgcaccgcatg1320

agccgacgcctgaaagccggttccgtcttcgtcaataactacaacgacggcgatatgacc1380

gtgccgtttggcggctataagcagagcggcaacggtcgcgacaaatccctgcatgccctt1440

gaaaaattcactgaactgaaaaccatctggataagcctggaggcctga1488

<210>40

<211>495

<212>prt

<213>unkown

<400>40

metasnphehishisleualatyrtrpglnasplysalaleuserleu

151015

alailegluasnargleupheileasnglyglutyrthralaalaala

202530

gluasngluthrphegluthrvalaspprovalthrglnalaproleu

354045

alalysilealaargglylysservalaspileaspargalametser

505560

alaalaargglyvalphegluargglyasptrpserleuserserpro

65707580

alalysarglysalavalleuasnlysleualaaspleumetgluala

859095

hisalaglugluleualaleuleugluthrleuaspthrglylyspro

100105110

ilearghisserleuargaspaspileproglyalaalaargalaile

115120125

argtrptyralaglualaileasplysvaltyrglygluvalalathr

130135140

thrserserhisgluleualametilevalarggluprovalglyval

145150155160

ilealaalailevalprotrpasnpheproleuleuleuthrcystrp

165170175

lysleuglyproalaleualaalaglyasnservalileleulyspro

180185190

serglulysserproleuseralaileargleualaglyleualalys

195200205

glualaglyleuproaspglyvalleuasnvalvalthrglyphegly

210215220

hisglualaglyglnalaleuserarghisasnaspileaspalaile

225230235240

alaphethrglyserthrargthrglylysglnleuleulysaspala

245250255

glyaspserasnmetlysargvaltrpleuglualaglyglylysser

260265270

alaasnilevalphealaaspcysproaspleuglnglnalaalaser

275280285

alathralaalaglyilephetyrasnglnglyglnvalcysileala

290295300

glythrargleuleuleuglugluserilealaaspglupheleuala

305310315320

leuleulysglnglnalaglnasntrpglnproglyhisproleuasp

325330335

proalathrthrmetglythrleuileaspcysalahisalaaspser

340345350

valhisserpheilearggluglygluserlysglyglnleuleuleu

355360365

aspglyargasnalaglyleualaalaalaileglyprothrilephe

370375380

valaspvalaspproasnalaserleuserargglugluilephegly

385390395400

provalleuvalvalthrargphethrserglugluglnalaleugln

405410415

leualaasnaspserglntyrglyleuglyalaalavaltrpthrarg

420425430

aspleuserargalahisargmetserargargleulysalaglyser

435440445

valphevalasnasntyrasnaspglyaspmetthrvalprophegly

450455460

glytyrlysglnserglyasnglyargasplysserleuhisalaleu

465470475480

glulysphethrgluleulysthriletrpileserleugluala

485490495

<210>41

<211>60

<212>dna

<213>unkown

<400>41

atggctatccctgcatttggtttaggtactttccgtctgagtgtaggctggagctgcttc60

<210>42

<211>62

<212>dna

<213>unkown

<400>42

ttaatcccattcaggagccagaccttccgggctaaccaggcggcatatgaatatcctcct60

ta62

<210>43

<211>62

<212>dna

<213>unkown

<400>43

atgcaacaaaaaatgattcaatttagtggcgatgtctcactggtgtaggctggagctgct60

tc62

<210>44

<211>62

<212>dna

<213>unkown

<400>44

tcacaccatatccagcgcagtttttccttttggtgccggatatcatatgaatatcctcct60

ta62

<210>45

<211>64

<212>dna

<213>unkown

<400>45

atgctacaaaattgcgcacaatcaaattgccgcattattcctaagtgtaggctggagctg60

cttc64

<210>46

<211>61

<212>dna

<213>unkown

<400>46

ttaattcccctgcatcgcccgcattcttgccgcatcttcccccatatgaatatcctcctt60

a61

<210>47

<211>61

<212>dna

<213>unkown

<400>47

atgaacaactttaatctgcacaccccaacccgcattctgttgtgtaggctggagctgctt60

c61

<210>48

<211>60

<212>dna

<213>unkown

<400>48

ttagcgggcggcttcgtatatacggcggctgacatccaacgcatatgaatatcctcctta60

<210>49

<211>62

<212>dna

<213>unkown

<400>49

atggctaatccaaccgttattaagctacaggatggcaatgtcgtgtaggctggagctgct60

tc62

<210>50

<211>60

<212>dna

<213>unkown

<400>50

ttagccgccgaactggtcaggatcgggaccgagacgcttgccatatgaatatcctcctta60

<210>51

<211>61

<212>dna

<213>unkown

<400>51

atgaagatcaaagctgttggtgcatattccgctaaacaaccgtgtaggctggagctgctt60

c61

<210>52

<211>60

<212>dna

<213>unkown

<400>52

tcagtctgttagtgtgcgattatcgataacaaaacgatattcatatgaatatcctcctta60

<210>53

<211>62

<212>dna

<213>unkown

<400>53

atgtcgatgataaaaagctatgccgcaaaagaagcgggcggcgtgtaggctggagctgct60

tc62

<210>54

<211>63

<212>dna

<213>unkown

<400>54

tcaaaaatcggctttcaacaccacgcggtaacgcgccttaccgtcatatgaatatcctcc60

tta63

<210>55

<211>49

<212>dna

<213>unkown

<400>55

gtatctagaaagaggagatataatgaattatcagaacgacgatttacgc49

<210>56

<211>35

<212>dna

<213>unkown

<400>56

tatggatccactagtttacccgcgacgcgctttta35

<210>57

<211>44

<212>dna

<213>unkown

<400>57

gtatctagaaagaggagatataatggttgctgaattgaccgcat44

<210>58

<211>38

<212>dna

<213>unkown

<400>58

atatgtcgacactagtttactggcgattgtcattcgcc38

<210>59

<211>53

<212>dna

<213>unkown

<400>59

atctctagatttaagaaggagatataatggcacctgttacaattgaaaagttc53

<210>60

<211>44

<212>dna

<213>unkown

<400>60

tctggatccgctagcctattttttatttcttttaagtgccgctg44

<210>61

<211>46

<212>dna

<213>unkown

<400>61

tagtctagatttaagaaggagatataatgacccccgaacagttccg46

<210>62

<211>38

<212>dna

<213>unkown

<400>62

acaggatccactagtttagctgccgcctttaatcacgt38

<210>63

<211>54

<212>dna

<213>unkown

<400>63

tactctagatttaagaaggagatataatgcgtaaagtgatcaaatatgatatgg54

<210>64

<211>38

<212>dna

<213>unkown

<400>64

tcaggatccactagtttagctgcccagtaaacgaggaa38

<210>65

<211>51

<212>dna

<213>unkown

<400>65

ttgtctagatttaagaaggagatataatggagaccgttagcaaaattgacc51

<210>66

<211>42

<212>dna

<213>unkown

<400>66

gcaggatccactagtttagtacggatatgcttcaatcacgct42

<210>67

<211>47

<212>dna

<213>unkown

<400>67

tactctagatttaagaaggagatataatggccttccatggcaaggac47

<210>68

<211>41

<212>dna

<213>unkown

<400>68

tcaggatccactagtttaaggaaacacctcaatcacgctct41

<210>69

<211>53

<212>dna

<213>unkown

<400>69

atctctagatttaagaaggagatataatgcagttaaaagaaaaagaggagctg53

<210>70

<211>46

<212>dna

<213>unkown

<400>70

agaggatccactagtttattttttcttaaacagcagatgggttaac46

<210>71

<211>48

<212>dna

<213>unkown

<400>71

tagtctagatttaagaaggagatataatgaaaccagaagatttccgcg48

<210>72

<211>33

<212>dna

<213>unkown

<400>72

aacgctcgagttaaatcgcagcttccatttcca33

<210>73

<211>47

<212>dna

<213>unkown

<400>73

atctctagatttaagaaggagatataatgagtccgatcacacgcgag47

<210>74

<211>36

<212>dna

<213>unkown

<400>74

agaggatccactagtttacagcaggcctaacaggcg36

<210>75

<211>55

<212>dna

<213>unkown

<400>75

gtctctagatttaagaaggagatataatgaagatttacggaatttatatggaccg55

<210>76

<211>45

<212>dna

<213>unkown

<400>76

tgaggatccactagtttataaaagctcttcgtacgagaccattgt45

<210>77

<211>52

<212>dna

<213>unkown

<400>77

atctctagatttaagaaggagatataatgaattttcatcatctggcttactg52

<210>78

<211>34

<212>dna

<213>unkown

<400>78

tcaggatccgctagctcaggcctccaggctatcc34