本发明属于蛋白质工程
技术领域：
：，具体涉及基于结构的crispr蛋白的优化设计方法，还涉及由该优化设计方法得到的spcas9的新型突变体，以及作为基因编辑工具的应用。
背景技术：
：：crispr是细菌和古细菌中抵抗外源核酸入侵的免疫系统。近年来，已被人们开发成一种特异性切割dna片段的基因编辑工具[1]。目前人们最常使用的是ii型crispr系统中的化脓性链球菌cas9（spcas9）[2]。但是，spcas9存在pam（protospaceradjacentmotifs）识别范围有限以及基因编辑时易脱靶的问题，从而使其更广泛应用受到限制[3-5]。因此，只有拓展pam识别范围和降低脱靶效应，才能使spcas9技术在基因编辑领域发挥更大的作用。为了达到以上目标，人们已提出多种研究方法：一种是利用细菌选择系统来筛选设计spcas9，通过该方法获得了pam识别范围扩大的vqr、eqr和vrer突变体[6]。另一种是基于spcas9结构指导的改造方法，也获得了高保真突变体espcas9、spcas9-hf、hypacas9和evocas9[7、8]。此外，还有人通过pace定向进化的方法获得了一个spcas9突变体（xcas9）。该突变体不仅有高特异性，而且pam识别更加灵活[9]。虽然，这些方法可以帮助人们获得cas9的新型突变体，但是这些方法盲目性比较大，且耗时耗力。随着计算机运算速度的快速增长，很多计算软件被用来辅助蛋白质设计。这个方法不受体系的限制，只要有蛋白质的三维结构，就可以将蛋白结构模型先精细化，再通过模拟自由能方法计算一些物理和化学过程的自由能变化，从而进行蛋白设计与改造。生物分子结构预测和设计的rosetta软件包（https://www.rosettacommons.org）包含140个应用程序，该软件可进行蛋白质结构从头预测、蛋白质结构设计和蛋白质重设计等[10、11]。用rosetta进行蛋白质设计的方法盲目性低，操作更简单，效率更高。基于上述问题，开发一种利用rosetta软件的优化设计crispr蛋白的方法十分有必要。技术实现要素：本发明的第一个目的是提供一种基于结构的crispr蛋白的优化设计方法，以获得spcas9的新型突变体。本发明的第二个目的是利用本发明方法获得spcas9的新型突变体（ycas9），为生物学领域提供有应用价值的新型基因编辑工具。本发明提供的基于结构的crispr蛋白的优化设计方法，从能量角度出发，使用rosetta来优化cas9蛋白与dna有相互作用的重要氨基酸，以增强cas9对dna的结合力，从而获得pam兼容性高或脱靶率低的突变体，记该突变体为ycas9。具体而言，本发明提供基于结构的crispr蛋白的优化设计方法，流程如附图1所示，其具体步骤如下。第一步：确定对cas9特性有重要影响的氨基酸位点。蛋白优化设计的第一步就是确定对cas9特性有重要影响的氨基酸位点。从蛋白质数据库（http://www.rcsb.org/）中下载蛋白结构，通过分析和比较蛋白的电子密度图，找出对cas9的pam识别或者脱靶作用有重要影响的氨基酸位点；具体地，xcas9突变的7个定向进化位点（a262t、r324l、s409i、e480k、e543d、m694i和e1219v）分布在sgrna-dna异源双链体的两侧（附图2）。这7个氨基酸共同作用，拓展了xcas9的pam识别范围并降低了其脱靶效应。因此，选取这7个位点作为rosetta优化设计的氨基酸位点。第二步：relax能量最小化。relax[12、13]是rosetta软件里结构预测中的一个模块。在rosetta力场中，使用该模块通过多次迭代使结构的氨基酸侧链重排，并且在每轮迭代中不断地调整范德华作用力，以能量最小化的方式搜索该结构的局部最优构象。所以，此步骤是将第一步中选择的结构用relax模块处理，使其处于能量最低的状态。本发明用relax模块对spcas9结构（pdbid:4un3）进行多轮（25轮）迭代，并根据rosetta的打分函数，选出能量最低的结构作为初始结构。第三步：fixbb优化protein-dna相互作用界面。fixbb（fixedbackbonedesign)[14、15]是rosetta软件里蛋白设计中一个模块。该模块可在固定蛋白主链骨架的同时，使侧链自由旋转，寻找其最佳的位置，并设计最适合该位置的氨基酸。所以，此步骤是把步骤二处理过的结构用fixbb模块固定它的蛋白骨架，然后针对步骤一中选出的氨基酸位点进行20种氨基酸随机组合设计，设置程序输出10000个组合结果。具体地，本发明使用fixbb模块固定第二步中筛选出的初始结构的主链原子，并对第一步中确定的7个氨基酸位点（262、324、409、480、543、694、1219）进行20种氨基酸组合突变设计，使程序输出10000个排列组合。第四步：去除重复性结果，筛选突变体。虽然每个氨基酸位点都有20种氨基酸可以选择，n个氨基酸位点就有20n种随机组合，但是适合该固定骨架且能量低的情况并不多，所以fixbb模块输出的结果中，会有很多重复的结果。此步骤就通过对输出的10000个结果进行序列比对，去除重复的结果，把剩余结果按能量从低到高的顺序排列，并从中进行挑选。最终选择分数值低即更接近自然构象的突变体，并将其命名为ycas9。第五步：突变体的表达与验证。将筛选到的突变体首先进行质粒构造，然后表达与纯化蛋白，最后对蛋白的剪切活性进行检测，以鉴定上述基于relax和fixbb等多种rosetta模块设计出的突变体的性能。本发明得到的ycas9，其氨基酸序列如seqidno.6所示，是将野生型spcas9的第409位的氨基酸s、第480位的e、第543位的e、第694位的m和第1219位的e分别突变成了n、k、d、l和t。ycas9具有和xcas9相当的基因编辑活性：宽泛的pam识别范围，可识别ngg、nga、ngt、ngc、gaa和gat，且在基因编辑时脱靶率低。本发明中，所述野生型spcas9核酸酶的核苷酸序列和氨基酸序列分别为seqidno.1和seqidno.2所示。所述xcas9核酸酶的核苷酸序列和氨基酸序列分别为seqidno.3和seqidno.4所示。所述ycas9核酸酶的核苷酸序列和氨基酸序列分别为seqidno.5和seqidno.6所示。本发明还提供一种多核苷酸序列，可以转录和翻译成所述的ycas9核酸酶，其序列为seqidno.7。本发明还提供一种表达载体，其含有上述多核苷酸序列。本发明还提供一种宿主细胞，可以用于转化上述表达载体。本发明还提供所述ycas9核酸酶的制备方法，具体步骤包括：首先，构建所述ycas9核酸酶的多核苷酸序列表达载体；然后，将所述表达载体转化至宿主细胞，筛选并挑出单克隆；最后，将所述单克隆诱导表达，并通过亲和层析从表达产物中分离出所述的ycas9核酸酶。本发明提供的上述crispr/cas9（ycas9）核酸酶、多核苷酸序列以及表达载体均可作为编辑基因组dna的编辑工具，用于基因组dna片段的相关编辑。本发明中，所述的基因编辑可以是单点编辑，也可以是编辑位点大于等于两个的多点编辑。所述编辑的手段包括删除、突变、插入、倒位、移位、重复或易位。本发明中，所述crispr/cas9编辑工具包括与靶标dna片段匹配的引导sgrna。所述的crispr/cas9核酸酶与能够介导它的sgrna组合，从而对目的基因进行编辑。附图说明图1为rosetta组合突变设计流程图。图2为xcas9定向进化位点的三维分布。图3为pet21-6his-tev-ycas9质粒构造。图4为含有ycas9的突变氨基酸的质粒测序。图5为crispr/cas9目标蛋白纯化的电泳鉴定。图6为sgrna的在靶和脱靶序列。图7为spcas9pam识别的检测。图8为xcas9pam识别的检测。图9为ycas9pam识别的检测。图10为spcas9脱靶效应的检测。图11为xcas9脱靶效应的检测。图12为ycas9脱靶效应的检测。具体实施方式下述实施例中所用的实验方法，如无特定说明，均为常规方法。下述实施例中所用的材料、试剂等，如无特定说明，均为从商业途径获得。一、spcas9的组合突变设计第一步：确定对cas9特性有重要影响的氨基酸位点。xcas9突变的7个定向进化位点（a262t、r324l、s409i、e480k、e543d、m694i和e1219v）分布在sgrna-dna异源双链体的两侧（附图2）。这7个氨基酸共同作用，拓展了xcas9的pam识别范围并降低了其脱靶效应。因此，选取这7个位点作为rosetta优化设计的氨基酸位点。第二步：relax能量最小化。用relax模块将spcas9结构（pdbid:4un3）进行25轮迭代，并根据rosetta的打分函数选出能量最低的结构作为初始结构。第三步：fixbb优化protein-dna相互作用界面。用fixbb模块固定第二步中筛选出的初始结构的主链原子，并对第一步中确定的7个氨基酸位点（262、324、409、480、543、694、1219）进行20种氨基酸组合突变设计，使程序输出10000个排列组合。第四步：去除重复性结果，筛选突变体。对这10000个组合进行序列比对，去除重复性结果，并将剩余结果按分数大小排列。最终选择分数值低即更接近自然构象的突变体，并将其命名为ycas9。第五步：突变体的表达与验证。本发明的ycas9是将野生型spcas9的第409位的氨基酸s、第480位的e、第543位的e、第694位的m和第1219位的e分别突变成了n、k、d、l和t。ycas9具有和xcas9相当的基因编辑活性。所述ycas9含有如seqidno.6所示的氨基酸序列。二、突变体ycas9的质粒构造、蛋白表达与纯化、检测（1）ycas9质粒构造：本文分别以质粒pet21-6his-tev-spcas9（seqidno.8）和pet21-6his-tev-xcas9（seqidno.9）为模板，通过一步定向克隆和点突变的方式构建突变体质粒，其具体质粒构建策略如附图3所示。质粒构建完成后，通过上海杰李生物技术有限公司测序，其结果如附图4所示，表明ycas9质粒构建成功。（2）ycas9蛋白表达与纯化：首先，将上述获得的ycas9质粒转入rosetta（de3）细胞中表达，当菌液的od600达到0.9时，加入终浓度为0.5μm的iptg，16℃过夜培养。其次，离心收集菌体，并用裂解液重悬菌体，超声破碎20min，12000rpm离心1h。然后，用ni柱亲和层析的方式纯化蛋白。最后，用sds-page鉴定，收集目的蛋白并将其浓缩至250μl，保存于蛋白储存液中。将浓缩后的蛋白再次进行电泳鉴定，结果如附图5所示，ycas9的杂蛋白已经非常少了，可用于后续实验。（3）底物dna的获取：底物dna序列如seqidno.10所示，通过pcr扩增和割胶回收的方式获得。底物dna为920bp，其中包含20bp靶序列和pam序列，pam序列又分别为5'-tgg-3'、5'-tga-3'、5'-tgc-3'、5'-tgt-3'、5'-gaa-3'和5'-gat-3'。原始底物dna的pam序列为tgg（seqidno.10），通过点突变的方式获得带有其他pam序列的dna。（4）sgrna的获取：sgrna序列如seqidno.11所示，通过pcr扩增，体外转录和纯化的方式获得。野生型sgrna（seqidno.11）中与底物dna的靶序列匹配的间隔序列为5'-uaccgcuccagucguucaug-3'（seqidno.12）。从pam远端开始依次将sgrna间隔序列的两个碱基突变为其互补碱基，共改8次，最终获得9个sgrna，其序列如附图6所示，这些sgrna将用于脱靶检测。（5）ycas9的pam识别检测：利用带有不同的pam序列的底物dna检测ycas9的pam识别能力，其反应体系为20μl体系：200nmcas9、200nmsgrna、30nmdna和反应缓冲液（20mmhepes,150mmkcl,1mmdtt,10mmmgcl2，ph7.5）。首先在反应缓冲液中加入cas9和sgrna，混合均匀后将其放置在37℃预孵育10min，然后将dna加入混合物中，继续孵育2h。反应结束后，使用琼脂糖凝胶电泳检测cas9的底物切割情况，其结果如附图7、8和9所示。由图可见，野生型spcas9主要识别tgg、tga和tgc，表明其可识别的pam的类型非常有限（图7，lanes2-4）。从图8可以看出，xcas9不仅可以识别tgg、tga和tgc，而且可以识别tgt、gaa和gat序列。而ycas9对包含这些pam序列的dna的剪切活性与xcas9相当（图9）。值得注意的是，ycas9对gat的识别能力比xcas9高（图9，lane7）。由此说明，rosetta设计的ycas9突变体的pam识别范围比野生型spcas9大，并且其对gat的识别能力优于xcas9。（6）ycas9的脱靶效应检测：利用不同的sgrna，如附图6中所示的0到8号sgrna检测ycas9的体外切割活性，从而评价ycas9的脱靶效应，其反应体系为20μl体系：200nmcas9、200nmsgrna、30nmdna和反应缓冲液（20mmhepes,150mmkcl,1mmdtt,10mmmgcl2，ph7.5）。首先在反应缓冲液中加入cas9和sgrna，混合均匀后将其放置在37℃预孵育10min，然后将dna加入混合物中，继续孵育3h。反应结束后，使用琼脂糖凝胶电泳检测cas9的底物切割情况，其结果如附图10、11和12所示。由图可见，1-8号sgrna均可以引导spcas9剪切底物dna。其中，1-5号sgrna引导的剪切活性与0号sgrna相当（图10，lanes3-8），这表明spcas9的脱靶效应比较严重。与spcas9相比，xcas9的脱靶效率明显降低。在1-8号sgrna的引导下，xcas9对底物的切割活性都有下降（图11，lanes3-11）。其中，泳道9和11的结果表明6号和8号sgrna几乎不引导xcas9切割底物dna。由图12可见，ycas9的脱靶效应与xcas9相当，在相同的反应时间内，ycas9对dna的剪切与xcas9几乎一致。因此，ycas9的脱靶率远远低于野生型spcas9。综上，ycas9突变体在功能上与xcas9相当，既拓展了pam识别能力，又降低了基因编辑时的脱靶效应，是一个有潜在应用价值的基因编辑工具，可以加强crispr系统介导的基因编辑技术的实用性和有效性。所以，本文所设计的基于结构的理性设计方法是可行有效的，为人们继续开发多pam识别和低脱靶的多功能型cas9蛋白提供了新的途径。参考文献[1]sanderjd,joungjk.crispr-cassystemsforediting,regulatingandtargetinggenomes[j].natbiotechnol,2014,32(4):347-355.[2]lierc,baticlee,horvathp,etal.analysisofthetypeii-acrispr-cassystemofstreptococcusagalactiaerevealsdistinctivefeaturesaccordingtogeneticlineages[j].frontgenet,2015,6:214.[3]hsupd,scottda,weinsteinja,etal.dnatargetingspecificityofrna-guidedcas9nucleases[j].natbiotechnol,2013,31(9):827-832.[4]fuy,sanderjd,reyond,etal.improvingcrispr-casnucleasespecificityusingtruncatedguidernas[j].natbiotechnol,2014,32(3):279-284.[5]congl,ranfa,coxd,etal.multiplexgenomeengineeringusingcrispr/cassystems[j].science,2013,339(6121):819-823.[6]kleinstiverbp,prewms,tsaisq,etal.engineeredcrispr/cas9nucleaseswithalteredpamspecificities[j].nature,2015,523(7561):481-485.[7]chenjs,dagdasys,kleinstiverbp,etal.enhancedproofreadinggovernscrispr/cas9targetingaccuracy[j].nature,2017,550(7676):407-410.[8]kleinstiverbp,pattanayakv,prewms,etal.high-fidelitycrispr/cas9nucleaseswithnodetectablegenome-wideoff-targeteffects[j].nature,2016,529(7587):490-495.[9]hujh,millersm,geurtsmh,etal.evolvedcas9variantswithbroadpamcompatibilityandhighdnaspecificity[j].nature,2018,556(7699):57-63.[10]leaver-faya,tykam,lewissm,etal.rosetta3:anobject-orientedsoftwaresuiteforthesimulationanddesignofmacromolecules[j].methodsenzymol,2011,487:545-574.[11]kerstins.broo,larsbrive,perahlberg,etal.catalysisofhydrolysisandtransesterifificationreactionsofp-nitrophenylestersbyadesignedhelix-loophelixdimer[j].amchemsoc,1997,119:11362–11372.[12]nivonlg,morettir,bakerd.apareto-optimalrefinementmethodforproteindesignscaffolds[j].plosone.2013,8(4):e59004.[13]conwayp,tykamd,dimaiof,etal.relaxationofbackbonebondgeometryimprovesproteinenergylandscapemodeling[j].proteinsci.2014,23(1):47-55.[14]kuhlmanb,dantasg,iretongc,etal.designofanovelglobularproteinfoldwithatomic-levelaccuracy[j].science.2003,302(5649):1364-1368.[15]hux,wangh,keh,etal.high-resolutiondesignofaproteinloop[j].procnatlacadsciusa.2007,104(45):17668-17673。序列表<110>复旦大学<120>一种基于结构的crispr蛋白的优化设计方法<130>001<160>12<170>siposequencelisting1.0<210>1<211>4104<212>dna<213>人工序列(artificialsequence)<400>1ggcgacaagaagtactccattgggctcgatatcggcacaaacagcgtcggctgggccgtc60attacggacgagtacaaggtgccgagcaaaaaattcaaagttctgggcaataccgatcgc120cacagcataaagaagaacctcattggcgccctcctgttcgactccggggagacggccgaa180gccacgcggctcaaaagaacagcacggcgcagatatacccgcagaaagaatcggatctgc240tacctgcaggagatctttagtaatgagatggctaaggtggatgactctttcttccatagg300ctggaggagtcctttttggtggaggaggataaaaagcacgagcgccacccaatctttggc360aatatcgtggacgaggtggcgtaccatgaaaagtacccaaccatatatcatctgaggaag420aagcttgtagacagtactgataaggctgacttgcggttgatctatctcgcgctggcgcat480atgatcaaatttcggggacacttcctcatcgagggggacctgaacccagacaacagcgat540gtcgacaaactctttatccaactggttcagacttacaatcagcttttcgaagagaacccg600atcaacgcatccggagttgacgccaaagcaatcctgagcgctaggctgtccaaatcccgg660cggctcgaaaacctcatcgcacagctccctggggagaagaagaacggcctgtttggtaat720cttatcgccctgtcactcgggctgacccccaactttaaatctaacttcgacctggccgaa780gatgccaagcttcaactgagcaaagacacctacgatgatgatctcgacaatctgctggcc840cagatcggcgaccagtacgcagacctttttttggcggcaaagaacctgtcagacgccatt900ctgctgagtgatattctgcgagtgaacacggagatcaccaaagctccgctgagcgctagt960atgatcaagcgctatgatgagcaccaccaagacttgactttgctgaaggcccttgtcaga1020cagcaactgcctgagaagtacaaggaaattttcttcgatcagtctaaaaatggctacgcc1080ggatacattgacggcggagcaagccaggaggaattttacaaatttattaagcccatcttg1140gaaaaaatggacggcaccgaggagctgctggtaaagcttaacagagaagatctgttgcgc1200aaacagcgcactttcgacaatggaagcatcccccaccagattcacctgggcgaactgcac1260gctatcctcaggcggcaagaggatttctacccctttttgaaagataacagggaaaagatt1320gagaaaatcctcacatttcggataccctactatgtaggccccctcgcccggggaaattcc1380agattcgcgtggatgactcgcaaatcagaagagaccatcactccctggaacttcgaggaa1440gtcgtggataagggggcctctgcccagtccttcatcgaaaggatgactaactttgataaa1500aatctgcctaacgaaaaggtgcttcctaaacactctctgctgtacgagtacttcacagtt1560tataacgagctcaccaaggtcaaatacgtcacagaagggatgagaaagccagcattcctg1620tctggagagcagaagaaagctatcgtggacctcctcttcaagacgaaccggaaagttacc1680gtgaaacagctcaaagaagactatttcaaaaagattgaatgtttcgactctgttgaaatc1740agcggagtggaggatcgcttcaacgcatccctgggaacgtatcacgatctcctgaaaatc1800attaaagacaaggacttcctggacaatgaggagaacgaggacattcttgaggacattgtc1860ctcacccttacgttgtttgaagatagggagatgattgaagaacgcttgaaaacttacgct1920catctcttcgacgacaaagtcatgaaacagctcaagaggcgccgatatacaggatggggg1980cggctgtcaagaaaactgatcaatgggatccgagacaagcagagtggaaagacaatcctg2040gattttcttaagtccgatggatttgccaaccggaacttcatgcagttgatccatgatgac2100tctctcacctttaaggaggacatccagaaagcacaagtttctggccagggggacagtctt2160cacgagcacatcgctaatcttgcaggtagcccagctatcaaaaagggaatactgcagacc2220gttaaggtcgtggatgaactcgtcaaagtaatgggaaggcataagcccgagaatatcgtt2280atcgagatggcccgagagaaccaaactacccagaagggacagaagaacagtagggaaagg2340atgaagaggattgaagagggtataaaagaactggggtcccaaatccttaaggaacaccca2400gttgaaaacacccagcttcagaatgagaagctctacctgtactacctgcagaacggcagg2460gacatgtacgtggatcaggaactggacatcaatcggctctccgactacgacgtggatcat2520atcgtgccccagtcttttctcaaagatgattctattgataataaagtgttgacaagatcc2580gataaaaatagagggaagagtgataacgtcccctcagaagaagttgtcaagaaaatgaaa2640aattattggcggcagctgctgaacgccaaactgatcacacaacggaagttcgataatctg2700actaaggctgaacgaggtggcctgtctgagttggataaagcaggcttcatcaaaaggcag2760cttgttgagacacgccagatcaccaagcacgtggcccaaattctcgattcacgcatgaac2820accaagtacgatgaaaatgacaaactgattcgagaggtgaaagttattactctgaagtct2880aagctggtctcagatttcagaaaggactttcagttttataaggtgagagagatcaacaat2940taccaccatgcgcatgatgcctacctgaatgcagtggtaggcactgcacttatcaaaaaa3000tatcccaagcttgaatctgaatttgtttacggagactataaagtgtacgatgttaggaaa3060atgatcgcaaagtctgagcaggaaataggcaaggccaccgctaagtacttcttttacagc3120aatattatgaattttttcaagaccgagattacactggccaatggagagattcggaagcga3180ccacttatcgaaacaaacggagaaacaggagaaatcgtgtgggacaagggtagggatttc3240gcgacagtccggaaggtcctgtccatgccgcaggtgaacatcgttaaaaagaccgaagta3300cagaccggaggcttctccaaggaaagtatcctcccgaaaaggaacagcgacaagctgatc3360gcacgcaaaaaagattgggaccccaagaaatacggcggattcgattctcctacagtcgct3420tacagtgtactggttgtggccaaagtggagaaagggaagtctaaaaaactcaaaagcgtc3480aaggaactgctgggcatcacaatcatggagcgatcaagcttcgaaaaaaaccccatcgac3540tttctcgaggcgaaaggatataaagaggtcaaaaaagacctcatcattaagcttcccaag3600tactctctctttgagcttgaaaacggccggaaacgaatgctcgctagtgcgggcgagctg3660cagaaaggtaacgagctggcactgccctctaaatacgttaatttcttgtatctggccagc3720cactatgaaaagctcaaagggtctcccgaagataatgagcagaagcagctgttcgtggaa3780caacacaaacactaccttgatgagatcatcgagcaaataagcgaattctccaaaagagtg3840atcctcgccgacgctaacctcgataaggtgctttctgcttacaataagcacagggataag3900cccatcagggagcaggcagaaaacattatccacttgtttactctgaccaacttgggcgcg3960cctgcagccttcaagtacttcgacaccaccatagacagaaagcggtacacctctacaaag4020gaggtcctggacgccacactgattcatcagtcaattacggggctctatgaaacaagaatc4080gacctctctcagctcggtggagac4104<210>2<211>1368<212>prt<213>人工序列(artificialsequence)<400>2glyasplyslystyrserileglyleuaspileglythrasnserval151015glytrpalavalilethraspglutyrlysvalproserlyslysphe202530lysvalleuglyasnthrasparghisserilelyslysasnleuile354045glyalaleuleupheaspserglygluthralaglualathrargleu505560lysargthralaargargargtyrthrargarglysasnargilecys65707580tyrleuglngluilepheserasnglumetalalysvalaspaspser859095phephehisargleuglugluserpheleuvalglugluasplyslys100105110hisgluarghisproilepheglyasnilevalaspgluvalalatyr115120125hisglulystyrprothriletyrhisleuarglyslysleuvalasp130135140serthrasplysalaaspleuargleuiletyrleualaleualahis145150155160metilelyspheargglyhispheleuilegluglyaspleuasnpro165170175aspasnseraspvalasplysleupheileglnleuvalglnthrtyr180185190asnglnleupheglugluasnproileasnalaserglyvalaspala195200205lysalaileleuseralaargleuserlysserargargleugluasn210215220leuilealaglnleuproglyglulyslysasnglyleupheglyasn225230235240leuilealaleuserleuglyleuthrproasnphelysserasnphe245250255aspleualagluaspalalysleuglnleuserlysaspthrtyrasp260265270aspaspleuaspasnleuleualaglnileglyaspglntyralaasp275280285leupheleualaalalysasnleuseraspalaileleuleuserasp290295300ileleuargvalasnthrgluilethrlysalaproleuseralaser305310315320metilelysargtyraspgluhishisglnaspleuthrleuleulys325330335alaleuvalargglnglnleuproglulystyrlysgluilephephe340345350aspglnserlysasnglytyralaglytyrileaspglyglyalaser355360365glnglugluphetyrlyspheilelysproileleuglulysmetasp370375380glythrglugluleuleuvallysleuasnarggluaspleuleuarg385390395400lysglnargthrpheaspasnglyserileprohisglnilehisleu405410415glygluleuhisalaileleuargargglngluaspphetyrprophe420425430leulysaspasnargglulysileglulysileleuthrpheargile435440445protyrtyrvalglyproleualaargglyasnserargphealatrp450455460metthrarglysserglugluthrilethrprotrpasnphegluglu465470475480valvalasplysglyalaseralaglnserpheilegluargmetthr485490495asnpheasplysasnleuproasnglulysvalleuprolyshisser500505510leuleutyrglutyrphethrvaltyrasngluleuthrlysvallys515520525tyrvalthrgluglymetarglysproalapheleuserglyglugln530535540lyslysalailevalaspleuleuphelysthrasnarglysvalthr545550555560vallysglnleulysgluasptyrphelyslysileglucyspheasp565570575servalgluileserglyvalgluaspargpheasnalaserleugly580585590thrtyrhisaspleuleulysileilelysasplysasppheleuasp595600605asnglugluasngluaspileleugluaspilevalleuthrleuthr610615620leuphegluaspargglumetileglugluargleulysthrtyrala625630635640hisleupheaspasplysvalmetlysglnleulysargargargtyr645650655thrglytrpglyargleuserarglysleuileasnglyileargasp660665670lysglnserglylysthrileleuasppheleulysseraspglyphe675680685alaasnargasnphemetglnleuilehisaspaspserleuthrphe690695700lysgluaspileglnlysalaglnvalserglyglnglyaspserleu705710715720hisgluhisilealaasnleualaglyserproalailelyslysgly725730735ileleuglnthrvallysvalvalaspgluleuvallysvalmetgly740745750arghislysprogluasnilevalileglumetalaarggluasngln755760765thrthrglnlysglyglnlysasnserarggluargmetlysargile770775780glugluglyilelysgluleuglyserglnileleulysgluhispro785790795800valgluasnthrglnleuglnasnglulysleutyrleutyrtyrleu805810815glnasnglyargaspmettyrvalaspglngluleuaspileasnarg820825830leuserasptyraspvalasphisilevalproglnserpheleulys835840845aspaspserileaspasnlysvalleuthrargserasplysasnarg850855860glylysseraspasnvalproserglugluvalvallyslysmetlys865870875880asntyrtrpargglnleuleuasnalalysleuilethrglnarglys885890895pheaspasnleuthrlysalagluargglyglyleusergluleuasp900905910lysalaglypheilelysargglnleuvalgluthrargglnilethr915920925lyshisvalalaglnileleuaspserargmetasnthrlystyrasp930935940gluasnasplysleuilearggluvallysvalilethrleulysser945950955960lysleuvalseraspphearglysasppheglnphetyrlysvalarg965970975gluileasnasntyrhishisalahisaspalatyrleuasnalaval980985990valglythralaleuilelyslystyrprolysleuglusergluphe99510001005valtyrglyasptyrlysvaltyraspvalarglysmetilealalys101010151020sergluglngluileglylysalathralalystyrphephetyrser1025103010351040asnilemetasnphephelysthrgluilethrleualaasnglyglu104510501055ilearglysargproleuilegluthrasnglygluthrglygluile106010651070valtrpasplysglyargaspphealathrvalarglysvalleuser107510801085metproglnvalasnilevallyslysthrgluvalglnthrglygly109010951100pheserlysgluserileleuprolysargasnserasplysleuile1105111011151120alaarglyslysasptrpaspprolyslystyrglyglypheaspser112511301135prothrvalalatyrservalleuvalvalalalysvalglulysgly114011451150lysserlyslysleulysservallysgluleuleuglyilethrile115511601165metgluargserserpheglulysasnproileasppheleugluala117011751180lysglytyrlysgluvallyslysaspleuileilelysleuprolys1185119011951200tyrserleuphegluleugluasnglyarglysargmetleualaser120512101215alaglygluleuglnlysglyasngluleualaleuproserlystyr122012251230valasnpheleutyrleualaserhistyrglulysleulysglyser123512401245progluaspasngluglnlysglnleuphevalgluglnhislyshis125012551260tyrleuaspgluileilegluglnileserglupheserlysargval1265127012751280ileleualaaspalaasnleuasplysvalleuseralatyrasnlys128512901295hisargasplysproilearggluglnalagluasnileilehisleu130013051310phethrleuthrasnleuglyalaproalaalaphelystyrpheasp131513201325thrthrileasparglysargtyrthrserthrlysgluvalleuasp133013351340alathrleuilehisglnserilethrglyleutyrgluthrargile1345135013551360aspleuserglnleuglyglyasp1365<210>3<211>4104<212>dna<213>人工序列(artificialsequence)<400>3ggcgacaagaagtactccattgggctcgatatcggcacaaacagcgtcggctgggccgtc60attacggacgagtacaaggtgccgagcaaaaaattcaaagttctgggcaataccgatcgc120cacagcataaagaagaacctcattggcgccctcctgttcgactccggggagacggccgaa180gccacgcggctcaaaagaacagcacggcgcagatatacccgcagaaagaatcggatctgc240tacctgcaggagatctttagtaatgagatggctaaggtggatgactctttcttccatagg300ctggaggagtcctttttggtggaggaggataaaaagcacgagcgccacccaatctttggc360aatatcgtggacgaggtggcgtaccatgaaaagtacccaaccatatatcatctgaggaag420aagcttgtagacagtactgataaggctgacttgcggttgatctatctcgcgctggcgcat480atgatcaaatttcggggacacttcctcatcgagggggacctgaacccagacaacagcgat540gtcgacaaactctttatccaactggttcagacttacaatcagcttttcgaagagaacccg600atcaacgcatccggagttgacgccaaagcaatcctgagcgctaggctgtccaaatcccgg660cggctcgaaaacctcatcgcacagctccctggggagaagaagaacggcctgtttggtaat720cttatcgccctgtcactcgggctgacccccaactttaaatctaacttcgacctggccgaa780gataccaagcttcaactgagcaaagacacctacgatgatgatctcgacaatctgctggcc840cagatcggcgaccagtacgcagacctttttttggcggcaaagaacctgtcagacgccatt900ctgctgagtgatattctgcgagtgaacacggagatcaccaaagctccgctgagcgctagt960atgatcaagctgtatgatgagcaccaccaagacttgactttgctgaaggcccttgtcaga1020cagcaactgcctgagaagtacaaggaaattttcttcgatcagtctaaaaatggctacgcc1080ggatacattgacggcggagcaagccaggaggaattttacaaatttattaagcccatcttg1140gaaaaaatggacggcaccgaggagctgctggtaaagcttaacagagaagatctgttgcgc1200aaacagcgcactttcgacaatggaattatcccccaccagattcacctgggcgaactgcac1260gctatcctcaggcggcaagaggatttctacccctttttgaaagataacagggaaaagatt1320gagaaaatcctcacatttcggataccctactatgtaggccccctcgcccggggaaattcc1380agattcgcgtggatgactcgcaaatcagaagagaccatcactccctggaacttcgagaaa1440gtcgtggataagggggcctctgcccagtccttcatcgaaaggatgactaactttgataaa1500aatctgcctaacgaaaaggtgcttcctaaacactctctgctgtacgagtacttcacagtt1560tataacgagctcaccaaggtcaaatacgtcacagaagggatgagaaagccagcattcctg1620tctggagatcagaagaaagctatcgtggacctcctcttcaagacgaaccggaaagttacc1680gtgaaacagctcaaagaagactatttcaaaaagattgaatgtttcgactctgttgaaatc1740agcggagtggaggatcgcttcaacgcatccctgggaacgtatcacgatctcctgaaaatc1800attaaagacaaggacttcctggacaatgaggagaacgaggacattcttgaggacattgtc1860ctcacccttacgttgtttgaagatagggagatgattgaagaacgcttgaaaacttacgct1920catctcttcgacgacaaagtcatgaaacagctcaagaggcgccgatatacaggatggggg1980cggctgtcaagaaaactgatcaatgggatccgagacaagcagagtggaaagacaatcctg2040gattttcttaagtccgatggatttgccaaccggaacttcattcagttgatccatgatgac2100tctctcacctttaaggaggacatccagaaagcacaagtttctggccagggggacagtctt2160cacgagcacatcgctaatcttgcaggtagcccagctatcaaaaagggaatactgcagacc2220gttaaggtcgtggatgaactcgtcaaagtaatgggaaggcataagcccgagaatatcgtt2280atcgagatggcccgagagaaccaaactacccagaagggacagaagaacagtagggaaagg2340atgaagaggattgaagagggtataaaagaactggggtcccaaatccttaaggaacaccca2400gttgaaaacacccagcttcagaatgagaagctctacctgtactacctgcagaacggcagg2460gacatgtacgtggatcaggaactggacatcaatcggctctccgactacgacgtggatcat2520atcgtgccccagtcttttctcaaagatgattctattgataataaagtgttgacaagatcc2580gataaaaatagagggaagagtgataacgtcccctcagaagaagttgtcaagaaaatgaaa2640aattattggcggcagctgctgaacgccaaactgatcacacaacggaagttcgataatctg2700actaaggctgaacgaggtggcctgtctgagttggataaagcaggcttcatcaaaaggcag2760cttgttgagacacgccagatcaccaagcacgtggcccaaattctcgattcacgcatgaac2820accaagtacgatgaaaatgacaaactgattcgagaggtgaaagttattactctgaagtct2880aagctggtctcagatttcagaaaggactttcagttttataaggtgagagagatcaacaat2940taccaccatgcgcatgatgcctacctgaatgcagtggtaggcactgcacttatcaaaaaa3000tatcccaagcttgaatctgaatttgtttacggagactataaagtgtacgatgttaggaaa3060atgatcgcaaagtctgagcaggaaataggcaaggccaccgctaagtacttcttttacagc3120aatattatgaattttttcaagaccgagattacactggccaatggagagattcggaagcga3180ccacttatcgaaacaaacggagaaacaggagaaatcgtgtgggacaagggtagggatttc3240gcgacagtccggaaggtcctgtccatgccgcaggtgaacatcgttaaaaagaccgaagta3300cagaccggaggcttctccaaggaaagtatcctcccgaaaaggaacagcgacaagctgatc3360gcacgcaaaaaagattgggaccccaagaaatacggcggattcgattctcctacagtcgct3420tacagtgtactggttgtggccaaagtggagaaagggaagtctaaaaaactcaaaagcgtc3480aaggaactgctgggcatcacaatcatggagcgatcaagcttcgaaaaaaaccccatcgac3540tttctcgaggcgaaaggatataaagaggtcaaaaaagacctcatcattaagcttcccaag3600tactctctctttgagcttgaaaacggccggaaacgaatgctcgctagtgcgggcgttctg3660cagaaaggtaacgagctggcactgccctctaaatacgttaatttcttgtatctggccagc3720cactatgaaaagctcaaagggtctcccgaagataatgagcagaagcagctgttcgtggaa3780caacacaaacactaccttgatgagatcatcgagcaaataagcgaattctccaaaagagtg3840atcctcgccgacgctaacctcgataaggtgctttctgcttacaataagcacagggataag3900cccatcagggagcaggcagaaaacattatccacttgtttactctgaccaacttgggcgcg3960cctgcagccttcaagtacttcgacaccaccatagacagaaagcggtacacctctacaaag4020gaggtcctggacgccacactgattcatcagtcaattacggggctctatgaaacaagaatc4080gacctctctcagctcggtggagac4104<210>4<211>1368<212>prt<213>人工序列(artificialsequence)<400>4glyasplyslystyrserileglyleuaspileglythrasnserval151015glytrpalavalilethraspglutyrlysvalproserlyslysphe202530lysvalleuglyasnthrasparghisserilelyslysasnleuile354045glyalaleuleupheaspserglygluthralaglualathrargleu505560lysargthralaargargargtyrthrargarglysasnargilecys65707580tyrleuglngluilepheserasnglumetalalysvalaspaspser859095phephehisargleuglugluserpheleuvalglugluasplyslys100105110hisgluarghisproilepheglyasnilevalaspgluvalalatyr115120125hisglulystyrprothriletyrhisleuarglyslysleuvalasp130135140serthrasplysalaaspleuargleuiletyrleualaleualahis145150155160metilelyspheargglyhispheleuilegluglyaspleuasnpro165170175aspasnseraspvalasplysleupheileglnleuvalglnthrtyr180185190asnglnleupheglugluasnproileasnalaserglyvalaspala195200205lysalaileleuseralaargleuserlysserargargleugluasn210215220leuilealaglnleuproglyglulyslysasnglyleupheglyasn225230235240leuilealaleuserleuglyleuthrproasnphelysserasnphe245250255aspleualagluaspthrlysleuglnleuserlysaspthrtyrasp260265270aspaspleuaspasnleuleualaglnileglyaspglntyralaasp275280285leupheleualaalalysasnleuseraspalaileleuleuserasp290295300ileleuargvalasnthrgluilethrlysalaproleuseralaser305310315320metilelysleutyraspgluhishisglnaspleuthrleuleulys325330335alaleuvalargglnglnleuproglulystyrlysgluilephephe340345350aspglnserlysasnglytyralaglytyrileaspglyglyalaser355360365glnglugluphetyrlyspheilelysproileleuglulysmetasp370375380glythrglugluleuleuvallysleuasnarggluaspleuleuarg385390395400lysglnargthrpheaspasnglyargileprohisglnilehisleu405410415glygluleuhisalaileleuargargglngluaspphetyrprophe420425430leulysaspasnargglulysileglulysileleuthrpheargile435440445protyrtyrvalglyproleualaargglyasnserargphealatrp450455460metthrarglysserglugluthrilethrprotrpasnpheglulys465470475480valvalasplysglyalaseralaglnserpheilegluargmetthr485490495asnpheasplysasnleuproasnglulysvalleuprolyshisser500505510leuleutyrglutyrphethrvaltyrasngluleuthrlysvallys515520525tyrvalthrgluglymetarglysproalapheleuserglyaspgln530535540lyslysalailevalaspleuleuphelysthrasnarglysvalthr545550555560vallysglnleulysgluasptyrphelyslysileglucyspheasp565570575servalgluileserglyvalgluaspargpheasnalaserleugly580585590thrtyrhisaspleuleulysileilelysasplysasppheleuasp595600605asnglugluasngluaspileleugluaspilevalleuthrleuthr610615620leuphegluaspargglumetileglugluargleulysthrtyrala625630635640hisleupheaspasplysvalmetlysglnleulysargargargtyr645650655thrglytrpglyargleuserarglysleuileasnglyileargasp660665670lysglnserglylysthrileleuasppheleulysseraspglyphe675680685alaasnargasnpheileglnleuilehisaspaspserleuthrphe690695700lysgluaspileglnlysalaglnvalserglyglnglyaspserleu705710715720hisgluhisilealaasnleualaglyserproalailelyslysgly725730735ileleuglnthrvallysvalvalaspgluleuvallysvalmetgly740745750arghislysprogluasnilevalileglumetalaarggluasngln755760765thrthrglnlysglyglnlysasnserarggluargmetlysargile770775780glugluglyilelysgluleuglyserglnileleulysgluhispro785790795800valgluasnthrglnleuglnasnglulysleutyrleutyrtyrleu805810815glnasnglyargaspmettyrvalaspglngluleuaspileasnarg820825830leuserasptyraspvalasphisilevalproglnserpheleulys835840845aspaspserileaspasnlysvalleuthrargserasplysasnarg850855860glylysseraspasnvalproserglugluvalvallyslysmetlys865870875880asntyrtrpargglnleuleuasnalalysleuilethrglnarglys885890895pheaspasnleuthrlysalagluargglyglyleusergluleuasp900905910lysalaglypheilelysargglnleuvalgluthrargglnilethr915920925lyshisvalalaglnileleuaspserargmetasnthrlystyrasp930935940gluasnasplysleuilearggluvallysvalilethrleulysser945950955960lysleuvalseraspphearglysasppheglnphetyrlysvalarg965970975gluileasnasntyrhishisalahisaspalatyrleuasnalaval980985990valglythralaleuilelyslystyrprolysleuglusergluphe99510001005valtyrglyasptyrlysvaltyraspvalarglysmetilealalys101010151020sergluglngluileglylysalathralalystyrphephetyrser1025103010351040asnilemetasnphephelysthrgluilethrleualaasnglyglu104510501055ilearglysargproleuilegluthrasnglygluthrglygluile106010651070valtrpasplysglyargaspphealathrvalarglysvalleuser107510801085metproglnvalasnilevallyslysthrgluvalglnthrglygly109010951100pheserlysgluserileleuprolysargasnserasplysleuile1105111011151120alaarglyslysasptrpaspprolyslystyrglyglypheaspser112511301135prothrvalalatyrservalleuvalvalalalysvalglulysgly114011451150lysserlyslysleulysservallysgluleuleuglyilethrile115511601165metgluargserserpheglulysasnproileasppheleugluala117011751180lysglytyrlysgluvallyslysaspleuileilelysleuprolys1185119011951200tyrserleuphegluleugluasnglyarglysargmetleualaser120512101215alaglygluleuglnlysglyasngluleualaleuproserlystyr122012251230valasnpheleutyrleualaserhistyrglulysleulysglyser123512401245progluaspasngluglnlysglnleuphevalgluglnhislyshis125012551260tyrleuaspgluileilegluglnileserglupheserlysargval1265127012751280ileleualaaspalaasnleuasplysvalleuseralatyrasnlys128512901295hisargasplysproilearggluglnalagluasnileilehisleu130013051310phethrleuthrasnleuglyalaproalaalaphelystyrpheasp131513201325thrthrileasparglysargtyrthrserthrlysgluvalleuasp133013351340alathrleuilehisglnserilethrglyleutyrgluthrargile1345135013551360aspleuserglnleuglyglyasp1365<210>5<211>4104<212>dna<213>人工序列(artificialsequence)<400>5ggcgacaagaagtactccattgggctcgatatcggcacaaacagcgtcggctgggccgtc60attacggacgagtacaaggtgccgagcaaaaaattcaaagttctgggcaataccgatcgc120cacagcataaagaagaacctcattggcgccctcctgttcgactccggggagacggccgaa180gccacgcggctcaaaagaacagcacggcgcagatatacccgcagaaagaatcggatctgc240tacctgcaggagatctttagtaatgagatggctaaggtggatgactctttcttccatagg300ctggaggagtcctttttggtggaggaggataaaaagcacgagcgccacccaatctttggc360aatatcgtggacgaggtggcgtaccatgaaaagtacccaaccatatatcatctgaggaag420aagcttgtagacagtactgataaggctgacttgcggttgatctatctcgcgctggcgcat480atgatcaaatttcggggacacttcctcatcgagggggacctgaacccagacaacagcgat540gtcgacaaactctttatccaactggttcagacttacaatcagcttttcgaagagaacccg600atcaacgcatccggagttgacgccaaagcaatcctgagcgctaggctgtccaaatcccgg660cggctcgaaaacctcatcgcacagctccctggggagaagaagaacggcctgtttggtaat720cttatcgccctgtcactcgggctgacccccaactttaaatctaacttcgacctggccgaa780gatgccaagcttcaactgagcaaagacacctacgatgatgatctcgacaatctgctggcc840cagatcggcgaccagtacgcagacctttttttggcggcaaagaacctgtcagacgccatt900ctgctgagtgatattctgcgagtgaacacggagatcaccaaagctccgctgagcgctagt960atgatcaagcgctatgatgagcaccaccaagacttgactttgctgaaggcccttgtcaga1020cagcaactgcctgagaagtacaaggaaattttcttcgatcagtctaaaaatggctacgcc1080ggatacattgacggcggagcaagccaggaggaattttacaaatttattaagcccatcttg1140gaaaaaatggacggcaccgaggagctgctggtaaagcttaacagagaagatctgttgcgc1200aaacagcgcactttcgacaatggaaatatcccccaccagattcacctgggcgaactgcac1260gctatcctcaggcggcaagaggatttctacccctttttgaaagataacagggaaaagatt1320gagaaaatcctcacatttcggataccctactatgtaggccccctcgcccggggaaattcc1380agattcgcgtggatgactcgcaaatcagaagagaccatcactccctggaacttcgagaaa1440gtcgtggataagggggcctctgcccagtccttcatcgaaaggatgactaactttgataaa1500aatctgcctaacgaaaaggtgcttcctaaacactctctgctgtacgagtacttcacagtt1560tataacgagctcaccaaggtcaaatacgtcacagaagggatgagaaagccagcattcctg1620tctggagatcagaagaaagctatcgtggacctcctcttcaagacgaaccggaaagttacc1680gtgaaacagctcaaagaagactatttcaaaaagattgaatgtttcgactctgttgaaatc1740agcggagtggaggatcgcttcaacgcatccctgggaacgtatcacgatctcctgaaaatc1800attaaagacaaggacttcctggacaatgaggagaacgaggacattcttgaggacattgtc1860ctcacccttacgttgtttgaagatagggagatgattgaagaacgcttgaaaacttacgct1920catctcttcgacgacaaagtcatgaaacagctcaagaggcgccgatatacaggatggggg1980cggctgtcaagaaaactgatcaatgggatccgagacaagcagagtggaaagacaatcctg2040gattttcttaagtccgatggatttgccaaccggaacttcctgcagttgatccatgatgac2100tctctcacctttaaggaggacatccagaaagcacaagtttctggccagggggacagtctt2160cacgagcacatcgctaatcttgcaggtagcccagctatcaaaaagggaatactgcagacc2220gttaaggtcgtggatgaactcgtcaaagtaatgggaaggcataagcccgagaatatcgtt2280atcgagatggcccgagagaaccaaactacccagaagggacagaagaacagtagggaaagg2340atgaagaggattgaagagggtataaaagaactggggtcccaaatccttaaggaacaccca2400gttgaaaacacccagcttcagaatgagaagctctacctgtactacctgcagaacggcagg2460gacatgtacgtggatcaggaactggacatcaatcggctctccgactacgacgtggatcat2520atcgtgccccagtcttttctcaaagatgattctattgataataaagtgttgacaagatcc2580gataaaaatagagggaagagtgataacgtcccctcagaagaagttgtcaagaaaatgaaa2640aattattggcggcagctgctgaacgccaaactgatcacacaacggaagttcgataatctg2700actaaggctgaacgaggtggcctgtctgagttggataaagcaggcttcatcaaaaggcag2760cttgttgagacacgccagatcaccaagcacgtggcccaaattctcgattcacgcatgaac2820accaagtacgatgaaaatgacaaactgattcgagaggtgaaagttattactctgaagtct2880aagctggtctcagatttcagaaaggactttcagttttataaggtgagagagatcaacaat2940taccaccatgcgcatgatgcctacctgaatgcagtggtaggcactgcacttatcaaaaaa3000tatcccaagcttgaatctgaatttgtttacggagactataaagtgtacgatgttaggaaa3060atgatcgcaaagtctgagcaggaaataggcaaggccaccgctaagtacttcttttacagc3120aatattatgaattttttcaagaccgagattacactggccaatggagagattcggaagcga3180ccacttatcgaaacaaacggagaaacaggagaaatcgtgtgggacaagggtagggatttc3240gcgacagtccggaaggtcctgtccatgccgcaggtgaacatcgttaaaaagaccgaagta3300cagaccggaggcttctccaaggaaagtatcctcccgaaaaggaacagcgacaagctgatc3360gcacgcaaaaaagattgggaccccaagaaatacggcggattcgattctcctacagtcgct3420tacagtgtactggttgtggccaaagtggagaaagggaagtctaaaaaactcaaaagcgtc3480aaggaactgctgggcatcacaatcatggagcgatcaagcttcgaaaaaaaccccatcgac3540tttctcgaggcgaaaggatataaagaggtcaaaaaagacctcatcattaagcttcccaag3600tactctctctttgagcttgaaaacggccggaaacgaatgctcgctagtgcgggcgcactg3660cagaaaggtaacgagctggcactgccctctaaatacgttaatttcttgtatctggccagc3720cactatgaaaagctcaaagggtctcccgaagataatgagcagaagcagctgttcgtggaa3780caacacaaacactaccttgatgagatcatcgagcaaataagcgaattctccaaaagagtg3840atcctcgccgacgctaacctcgataaggtgctttctgcttacaataagcacagggataag3900cccatcagggagcaggcagaaaacattatccacttgtttactctgaccaacttgggcgcg3960cctgcagccttcaagtacttcgacaccaccatagacagaaagcggtacacctctacaaag4020gaggtcctggacgccacactgattcatcagtcaattacggggctctatgaaacaagaatc4080gacctctctcagctcggtggagac4104<210>6<211>1368<212>prt<213>人工序列(artificialsequence)<400>6glyasplyslystyrserileglyleuaspileglythrasnserval151015glytrpalavalilethraspglutyrlysvalproserlyslysphe202530lysvalleuglyasnthrasparghisserilelyslysasnleuile354045glyalaleuleupheaspserglygluthralaglualathrargleu505560lysargthralaargargargtyrthrargarglysasnargilecys65707580tyrleuglngluilepheserasnglumetalalysvalaspaspser859095phephehisargleuglugluserpheleuvalglugluasplyslys100105110hisgluarghisproilepheglyasnilevalaspgluvalalatyr115120125hisglulystyrprothriletyrhisleuarglyslysleuvalasp130135140serthrasplysalaaspleuargleuiletyrleualaleualahis145150155160metilelyspheargglyhispheleuilegluglyaspleuasnpro165170175aspasnseraspvalasplysleupheileglnleuvalglnthrtyr180185190asnglnleupheglugluasnproileasnalaserglyvalaspala195200205lysalaileleuseralaargleuserlysserargargleugluasn210215220leuilealaglnleuproglyglulyslysasnglyleupheglyasn225230235240leuilealaleuserleuglyleuthrproasnphelysserasnphe245250255aspleualagluaspalalysleuglnleuserlysaspthrtyrasp260265270aspaspleuaspasnleuleualaglnileglyaspglntyralaasp275280285leupheleualaalalysasnleuseraspalaileleuleuserasp290295300ileleuargvalasnthrgluilethrlysalaproleuseralaser305310315320metilelysargtyraspgluhishisglnaspleuthrleuleulys325330335alaleuvalargglnglnleuproglulystyrlysgluilephephe340345350aspglnserlysasnglytyralaglytyrileaspglyglyalaser355360365glnglugluphetyrlyspheilelysproileleuglulysmetasp370375380glythrglugluleuleuvallysleuasnarggluaspleuleuarg385390395400lysglnargthrpheaspasnglyasnileprohisglnilehisleu405410415glygluleuhisalaileleuargargglngluaspphetyrprophe420425430leulysaspasnargglulysileglulysileleuthrpheargile435440445protyrtyrvalglyproleualaargglyasnserargphealatrp450455460metthrarglysserglugluthrilethrprotrpasnpheglulys465470475480valvalasplysglyalaseralaglnserpheilegluargmetthr485490495asnpheasplysasnleuproasnglulysvalleuprolyshisser500505510leuleutyrglutyrphethrvaltyrasngluleuthrlysvallys515520525tyrvalthrgluglymetarglysproalapheleuserglyaspgln530535540lyslysalailevalaspleuleuphelysthrasnarglysvalthr545550555560vallysglnleulysgluasptyrphelyslysileglucyspheasp565570575servalgluileserglyvalgluaspargpheasnalaserleugly580585590thrtyrhisaspleuleulysileilelysasplysasppheleuasp595600605asnglugluasngluaspileleugluaspilevalleuthrleuthr610615620leuphegluaspargglumetileglugluargleulysthrtyrala625630635640hisleupheaspasplysvalmetlysglnleulysargargargtyr645650655thrglytrpglyargleuserarglysleuileasnglyileargasp660665670lysglnserglylysthrileleuasppheleulysseraspglyphe675680685alaasnargasnpheleuglnleuilehisaspaspserleuthrphe690695700lysgluaspileglnlysalaglnvalserglyglnglyaspserleu705710715720hisgluhisilealaasnleualaglyserproalailelyslysgly725730735ileleuglnthrvallysvalvalaspgluleuvallysvalmetgly740745750arghislysprogluasnilevalileglumetalaarggluasngln755760765thrthrglnlysglyglnlysasnserarggluargmetlysargile770775780glugluglyilelysgluleuglyserglnileleulysgluhispro785790795800valgluasnthrglnleuglnasnglulysleutyrleutyrtyrleu805810815glnasnglyargaspmettyrvalaspglngluleuaspileasnarg820825830leuserasptyraspvalasphisilevalproglnserpheleulys835840845aspaspserileaspasnlysvalleuthrargserasplysasnarg850855860glylysseraspasnvalproserglugluvalvallyslysmetlys865870875880asntyrtrpargglnleuleuasnalalysleuilethrglnarglys885890895pheaspasnleuthrlysalagluargglyglyleusergluleuasp900905910lysalaglypheilelysargglnleuvalgluthrargglnilethr915920925lyshisvalalaglnileleuaspserargmetasnthrlystyrasp930935940gluasnasplysleuilearggluvallysvalilethrleulysser945950955960lysleuvalseraspphearglysasppheglnphetyrlysvalarg965970975gluileasnasntyrhishisalahisaspalatyrleuasnalaval980985990valglythralaleuilelyslystyrprolysleuglusergluphe99510001005valtyrglyasptyrlysvaltyraspvalarglysmetilealalys101010151020sergluglngluileglylysalathralalystyrphephetyrser1025103010351040asnilemetasnphephelysthrgluilethrleualaasnglyglu104510501055ilearglysargproleuilegluthrasnglygluthrglygluile106010651070valtrpasplysglyargaspphealathrvalarglysvalleuser107510801085metproglnvalasnilevallyslysthrgluvalglnthrglygly109010951100pheserlysgluserileleuprolysargasnserasplysleuile1105111011151120alaarglyslysasptrpaspprolyslystyrglyglypheaspser112511301135prothrvalalatyrservalleuvalvalalalysvalglulysgly114011451150lysserlyslysleulysservallysgluleuleuglyilethrile115511601165metgluargserserpheglulysasnproileasppheleugluala117011751180lysglytyrlysgluvallyslysaspleuileilelysleuprolys1185119011951200tyrserleuphegluleugluasnglyarglysargmetleualaser120512101215alaglythrleuglnlysglyasngluleualaleuproserlystyr122012251230valasnpheleutyrleualaserhistyrglulysleulysglyser123512401245progluaspasngluglnlysglnleuphevalgluglnhislyshis125012551260tyrleuaspgluileilegluglnileserglupheserlysargval1265127012751280ileleualaaspalaasnleuasplysvalleuseralatyrasnlys128512901295hisargasplysproilearggluglnalagluasnileilehisleu130013051310phethrleuthrasnleuglyalaproalaalaphelystyrpheasp131513201325thrthrileasparglysargtyrthrserthrlysgluvalleuasp133013351340alathrleuilehisglnserilethrglyleutyrgluthrargile1345135013551360aspleuserglnleuglyglyasp1365<210>7<211>9553<212>dna<213>人工序列(artificialsequence)<400>7tggcgaatgggacgcgccctgtagcggcgcattaagcgcggcgggtgtggtggttacgcg60cagcgtgaccgctacacttgccagcgccctagcgcccgctcctttcgctttcttcccttc120ctttctcgccacgttcgccggctttccccgtcaagctctaaatcgggggctccctttagg180gttccgatttagtgctttacggcacctcgaccccaaaaaacttgattagggtgatggttc240acgtagtgggccatcgccctgatagacggtttttcgccctttgacgttggagtccacgtt300ctttaatagtggactcttgttccaaactggaacaacactcaaccctatctcggtctattc360ttttgatttataagggattttgccgatttcggcctattggttaaaaaatgagctgattta420acaaaaatttaacgcgaattttaacaaaatattaacgtttacaatttcaggtggcacttt480tcggggaaatgtgcgcggaacccctatttgtttatttttctaaatacattcaaatatgta540tccgctcatgagacaataaccctgataaatgcttcaataatattgaaaaaggaagagtat600gagtattcaacatttccgtgtcgcccttattcccttttttgcggcattttgccttcctgt660ttttgctcacccagaaacgctggtgaaagtaaaagatgctgaagatcagttgggtgcacg720agtgggttacatcgaactggatctcaacagcggtaagatccttgagagttttcgccccga780agaacgttttccaatgatgagcacttttaaagttctgctatgtggcgcggtattatcccg840tattgacgccgggcaagagcaactcggtcgccgcatacactattctcagaatgacttggt900tgagtactcaccagtcacagaaaagcatcttacggatggcatgacagtaagagaattatg960cagtgctgccataaccatgagtgataacactgcggccaacttacttctgacaacgatcgg1020aggaccgaaggagctaaccgcttttttgcacaacatgggggatcatgtaactcgccttga1080tcgttgggaaccggagctgaatgaagccataccaaacgacgagcgtgacaccacgatgcc1140tgcagcaatggcaacaacgttgcgcaaactattaactggcgaactacttactctagcttc1200ccggcaacaattaatagactggatggaggcggataaagttgcaggaccacttctgcgctc1260ggcccttccggctggctggtttattgctgataaatctggagccggtgagcgtgggtctcg1320cggtatcattgcagcactggggccagatggtaagccctcccgtatcgtagttatctacac1380gacggggagtcaggcaactatggatgaacgaaatagacagatcgctgagataggtgcctc1440actgattaagcattggtaactgtcagaccaagtttactcatatatactttagattgattt1500aaaacttcatttttaatttaaaaggatctaggtgaagatcctttttgataatctcatgac1560caaaatcccttaacgtgagttttcgttccactgagcgtcagaccccgtagaaaagatcaa1620aggatcttcttgagatcctttttttctgcgcgtaatctgctgcttgcaaacaaaaaaacc1680accgctaccagcggtggtttgtttgccggatcaagagctaccaactctttttccgaaggt1740aactggcttcagcagagcgcagataccaaatactgtccttctagtgtagccgtagttagg1800ccaccacttcaagaactctgtagcaccgcctacatacctcgctctgctaatcctgttacc1860agtggctgctgccagtggcgataagtcgtgtcttaccgggttggactcaagacgatagtt1920accggataaggcgcagcggtcgggctgaacggggggttcgtgcacacagcccagcttgga1980gcgaacgacctacaccgaactgagatacctacagcgtgagctatgagaaagcgccacgct2040tcccgaagggagaaaggcggacaggtatccggtaagcggcagggtcggaacaggagagcg2100cacgagggagcttccagggggaaacgcctggtatctttatagtcctgtcgggtttcgcca2160cctctgacttgagcgtcgatttttgtgatgctcgtcaggggggcggagcctatggaaaaa2220cgccagcaacgcggcctttttacggttcctggccttttgctggccttttgctcacatgtt2280ctttcctgcgttatcccctgattctgtggataaccgtattaccgcctttgagtgagctga2340taccgctcgccgcagccgaacgaccgagcgcagcgagtcagtgagcgaggaagcggaaga2400gcgcctgatgcggtattttctccttacgcatctgtgcggtatttcacaccgcatatatgg2460tgcactctcagtacaatctgctctgatgccgcatagttaagccagtatacactccgctat2520cgctacgtgactgggtcatggctgcgccccgacacccgccaacacccgctgacgcgccct2580gacgggcttgtctgctcccggcatccgcttacagacaagctgtgaccgtctccgggagct2640gcatgtgtcagaggttttcaccgtcatcaccgaaacgcgcgaggcagctgcggtaaagct2700catcagcgtggtcgtgaagcgattcacagatgtctgcctgttcatccgcgtccagctcgt2760tgagtttctccagaagcgttaatgtctggcttctgataaagcgggccatgttaagggcgg2820ttttttcctgtttggtcactgatgcctccgtgtaagggggatttctgttcatgggggtaa2880tgataccgatgaaacgagagaggatgctcacgatacgggttactgatgatgaacatgccc2940ggttactggaacgttgtgagggtaaacaactggcggtatggatgcggcgggaccagagaa3000aaatcactcagggtcaatgccagcgcttcgttaatacagatgtaggtgttccacagggta3060gccagcagcatcctgcgatgcagatccggaacataatggtgcagggcgctgacttccgcg3120tttccagactttacgaaacacggaaaccgaagaccattcatgttgttgctcaggtcgcag3180acgttttgcagcagcagtcgcttcacgttcgctcgcgtatcggtgattcattctgctaac3240cagtaaggcaaccccgccagcctagccgggtcctcaacgacaggagcacgatcatgcgca3300cccgtggggccgccatgccggcgataatggcctgcttctcgccgaaacgtttggtggcgg3360gaccagtgacgaaggcttgagcgagggcgtgcaagattccgaataccgcaagcgacaggc3420cgatcatcgtcgcgctccagcgaaagcggtcctcgccgaaaatgacccagagcgctgccg3480gcacctgtcctacgagttgcatgataaagaagacagtcataagtgcggcgacgatagtca3540tgccccgcgcccaccggaaggagctgactgggttgaaggctctcaagggcatcggtcgag3600atcccggtgcctaatgagtgagctaacttacattaattgcgttgcgctcactgcccgctt3660tccagtcgggaaacctgtcgtgccagctgcattaatgaatcggccaacgcgcggggagag3720gcggtttgcgtattgggcgccagggtggtttttcttttcaccagtgagacgggcaacagc3780tgattgcccttcaccgcctggccctgagagagttgcagcaagcggtccacgctggtttgc3840cccagcaggcgaaaatcctgtttgatggtggttaacggcgggatataacatgagctgtct3900tcggtatcgtcgtatcccactaccgagatatccgcaccaacgcgcagcccggactcggta3960atggcgcgcattgcgcccagcgccatctgatcgttggcaaccagcatcgcagtgggaacg4020atgccctcattcagcatttgcatggtttgttgaaaaccggacatggcactccagtcgcct4080tcccgttccgctatcggctgaatttgattgcgagtgagatatttatgccagccagccaga4140cgcagacgcgccgagacagaacttaatgggcccgctaacagcgcgatttgctggtgaccc4200aatgcgaccagatgctccacgcccagtcgcgtaccgtcttcatgggagaaaataatactg4260ttgatgggtgtctggtcagagacatcaagaaataacgccggaacattagtgcaggcagct4320tccacagcaatggcatcctggtcatccagcggatagttaatgatcagcccactgacgcgt4380tgcgcgagaagattgtgcaccgccgctttacaggcttcgacgccgcttcgttctaccatc4440gacaccaccacgctggcacccagttgatcggcgcgagatttaatcgccgcgacaatttgc4500gacggcgcgtgcagggccagactggaggtggcaacgccaatcagcaacgactgtttgccc4560gccagttgttgtgccacgcggttgggaatgtaattcagctccgccatcgccgcttccact4620ttttcccgcgttttcgcagaaacgtggctggcctggttcaccacgcgggaaacggtctga4680taagagacaccggcatactctgcgacatcgtataacgttactggtttcacattcaccacc4740ctgaattgactctcttccgggcgctatcatgccataccgcgaaaggttttgcgccattcg4800atggtgtccgggatctcgacgctctcccttatgcgactcctgcattaggaagcagcccag4860tagtaggttgaggccgttgagcaccgccgccgcaaggaatggtgcatgcaaggagatggc4920gcccaacagtcccccggccacggggcctgccaccatacccacgccgaaacaagcgctcat4980gagcccgaagtggcgagcccgatcttccccatcggtgatgtcggcgatataggcgccagc5040aaccgcacctgtggcgccggtgatgccggccacgatgcgtccggcgtagaggatcgagat5100ctcgatcccgcgaaattaatacgactcactataggggaattgtgagcggataacaattcc5160cctctagaaataattttgtttaactttaagaaggagatataccatgggcagcagccatca5220tcatcatcatcacagcagcggcctggtgccgcgcggcagccatatggaaaatctctactt5280ccaaggcgacaagaagtactccattgggctcgatatcggcacaaacagcgtcggctgggc5340cgtcattacggacgagtacaaggtgccgagcaaaaaattcaaagttctgggcaataccga5400tcgccacagcataaagaagaacctcattggcgccctcctgttcgactccggggagacggc5460cgaagccacgcggctcaaaagaacagcacggcgcagatatacccgcagaaagaatcggat5520ctgctacctgcaggagatctttagtaatgagatggctaaggtggatgactctttcttcca5580taggctggaggagtcctttttggtggaggaggataaaaagcacgagcgccacccaatctt5640tggcaatatcgtggacgaggtggcgtaccatgaaaagtacccaaccatatatcatctgag5700gaagaagcttgtagacagtactgataaggctgacttgcggttgatctatctcgcgctggc5760gcatatgatcaaatttcggggacacttcctcatcgagggggacctgaacccagacaacag5820cgatgtcgacaaactctttatccaactggttcagacttacaatcagcttttcgaagagaa5880cccgatcaacgcatccggagttgacgccaaagcaatcctgagcgctaggctgtccaaatc5940ccggcggctcgaaaacctcatcgcacagctccctggggagaagaagaacggcctgtttgg6000taatcttatcgccctgtcactcgggctgacccccaactttaaatctaacttcgacctggc6060cgaagatgccaagcttcaactgagcaaagacacctacgatgatgatctcgacaatctgct6120ggcccagatcggcgaccagtacgcagacctttttttggcggcaaagaacctgtcagacgc6180cattctgctgagtgatattctgcgagtgaacacggagatcaccaaagctccgctgagcgc6240tagtatgatcaagcgctatgatgagcaccaccaagacttgactttgctgaaggcccttgt6300cagacagcaactgcctgagaagtacaaggaaattttcttcgatcagtctaaaaatggcta6360cgccggatacattgacggcggagcaagccaggaggaattttacaaatttattaagcccat6420cttggaaaaaatggacggcaccgaggagctgctggtaaagcttaacagagaagatctgtt6480gcgcaaacagcgcactttcgacaatggaaatatcccccaccagattcacctgggcgaact6540gcacgctatcctcaggcggcaagaggatttctacccctttttgaaagataacagggaaaa6600gattgagaaaatcctcacatttcggataccctactatgtaggccccctcgcccggggaaa6660ttccagattcgcgtggatgactcgcaaatcagaagagaccatcactccctggaacttcga6720gaaagtcgtggataagggggcctctgcccagtccttcatcgaaaggatgactaactttga6780taaaaatctgcctaacgaaaaggtgcttcctaaacactctctgctgtacgagtacttcac6840agtttataacgagctcaccaaggtcaaatacgtcacagaagggatgagaaagccagcatt6900cctgtctggagatcagaagaaagctatcgtggacctcctcttcaagacgaaccggaaagt6960taccgtgaaacagctcaaagaagactatttcaaaaagattgaatgtttcgactctgttga7020aatcagcggagtggaggatcgcttcaacgcatccctgggaacgtatcacgatctcctgaa7080aatcattaaagacaaggacttcctggacaatgaggagaacgaggacattcttgaggacat7140tgtcctcacccttacgttgtttgaagatagggagatgattgaagaacgcttgaaaactta7200cgctcatctcttcgacgacaaagtcatgaaacagctcaagaggcgccgatatacaggatg7260ggggcggctgtcaagaaaactgatcaatgggatccgagacaagcagagtggaaagacaat7320cctggattttcttaagtccgatggatttgccaaccggaacttcctgcagttgatccatga7380tgactctctcacctttaaggaggacatccagaaagcacaagtttctggccagggggacag7440tcttcacgagcacatcgctaatcttgcaggtagcccagctatcaaaaagggaatactgca7500gaccgttaaggtcgtggatgaactcgtcaaagtaatgggaaggcataagcccgagaatat7560cgttatcgagatggcccgagagaaccaaactacccagaagggacagaagaacagtaggga7620aaggatgaagaggattgaagagggtataaaagaactggggtcccaaatccttaaggaaca7680cccagttgaaaacacccagcttcagaatgagaagctctacctgtactacctgcagaacgg7740cagggacatgtacgtggatcaggaactggacatcaatcggctctccgactacgacgtgga7800tcatatcgtgccccagtcttttctcaaagatgattctattgataataaagtgttgacaag7860atccgataaaaatagagggaagagtgataacgtcccctcagaagaagttgtcaagaaaat7920gaaaaattattggcggcagctgctgaacgccaaactgatcacacaacggaagttcgataa7980tctgactaaggctgaacgaggtggcctgtctgagttggataaagcaggcttcatcaaaag8040gcagcttgttgagacacgccagatcaccaagcacgtggcccaaattctcgattcacgcat8100gaacaccaagtacgatgaaaatgacaaactgattcgagaggtgaaagttattactctgaa8160gtctaagctggtctcagatttcagaaaggactttcagttttataaggtgagagagatcaa8220caattaccaccatgcgcatgatgcctacctgaatgcagtggtaggcactgcacttatcaa8280aaaatatcccaagcttgaatctgaatttgtttacggagactataaagtgtacgatgttag8340gaaaatgatcgcaaagtctgagcaggaaataggcaaggccaccgctaagtacttctttta8400cagcaatattatgaattttttcaagaccgagattacactggccaatggagagattcggaa8460gcgaccacttatcgaaacaaacggagaaacaggagaaatcgtgtgggacaagggtaggga8520tttcgcgacagtccggaaggtcctgtccatgccgcaggtgaacatcgttaaaaagaccga8580agtacagaccggaggcttctccaaggaaagtatcctcccgaaaaggaacagcgacaagct8640gatcgcacgcaaaaaagattgggaccccaagaaatacggcggattcgattctcctacagt8700cgcttacagtgtactggttgtggccaaagtggagaaagggaagtctaaaaaactcaaaag8760cgtcaaggaactgctgggcatcacaatcatggagcgatcaagcttcgaaaaaaaccccat8820cgactttctcgaggcgaaaggatataaagaggtcaaaaaagacctcatcattaagcttcc8880caagtactctctctttgagcttgaaaacggccggaaacgaatgctcgctagtgcgggcgc8940actgcagaaaggtaacgagctggcactgccctctaaatacgttaatttcttgtatctggc9000cagccactatgaaaagctcaaagggtctcccgaagataatgagcagaagcagctgttcgt9060ggaacaacacaaacactaccttgatgagatcatcgagcaaataagcgaattctccaaaag9120agtgatcctcgccgacgctaacctcgataaggtgctttctgcttacaataagcacaggga9180taagcccatcagggagcaggcagaaaacattatccacttgtttactctgaccaacttggg9240cgcgcctgcagccttcaagtacttcgacaccaccatagacagaaagcggtacacctctac9300aaaggaggtcctggacgccacactgattcatcagtcaattacggggctctatgaaacaag9360aatcgacctctctcagctcggtggagactgactcgagcaccaccaccaccaccactgaga9420tccggctgctaacaaagcccgaaaggaagctgagttggctgctgccaccgctgagcaata9480actagcataaccccttggggcctctaaacgggtcttgaggggttttttgctgaaaggagg9540aactatatccgga9553<210>8<211>9553<212>dna<213>人工序列(artificialsequence)<400>8tggcgaatgggacgcgccctgtagcggcgcattaagcgcggcgggtgtggtggttacgcg60cagcgtgaccgctacacttgccagcgccctagcgcccgctcctttcgctttcttcccttc120ctttctcgccacgttcgccggctttccccgtcaagctctaaatcgggggctccctttagg180gttccgatttagtgctttacggcacctcgaccccaaaaaacttgattagggtgatggttc240acgtagtgggccatcgccctgatagacggtttttcgccctttgacgttggagtccacgtt300ctttaatagtggactcttgttccaaactggaacaacactcaaccctatctcggtctattc360ttttgatttataagggattttgccgatttcggcctattggttaaaaaatgagctgattta420acaaaaatttaacgcgaattttaacaaaatattaacgtttacaatttcaggtggcacttt480tcggggaaatgtgcgcggaacccctatttgtttatttttctaaatacattcaaatatgta540tccgctcatgagacaataaccctgataaatgcttcaataatattgaaaaaggaagagtat600gagtattcaacatttccgtgtcgcccttattcccttttttgcggcattttgccttcctgt660ttttgctcacccagaaacgctggtgaaagtaaaagatgctgaagatcagttgggtgcacg720agtgggttacatcgaactggatctcaacagcggtaagatccttgagagttttcgccccga780agaacgttttccaatgatgagcacttttaaagttctgctatgtggcgcggtattatcccg840tattgacgccgggcaagagcaactcggtcgccgcatacactattctcagaatgacttggt900tgagtactcaccagtcacagaaaagcatcttacggatggcatgacagtaagagaattatg960cagtgctgccataaccatgagtgataacactgcggccaacttacttctgacaacgatcgg1020aggaccgaaggagctaaccgcttttttgcacaacatgggggatcatgtaactcgccttga1080tcgttgggaaccggagctgaatgaagccataccaaacgacgagcgtgacaccacgatgcc1140tgcagcaatggcaacaacgttgcgcaaactattaactggcgaactacttactctagcttc1200ccggcaacaattaatagactggatggaggcggataaagttgcaggaccacttctgcgctc1260ggcccttccggctggctggtttattgctgataaatctggagccggtgagcgtgggtctcg1320cggtatcattgcagcactggggccagatggtaagccctcccgtatcgtagttatctacac1380gacggggagtcaggcaactatggatgaacgaaatagacagatcgctgagataggtgcctc1440actgattaagcattggtaactgtcagaccaagtttactcatatatactttagattgattt1500aaaacttcatttttaatttaaaaggatctaggtgaagatcctttttgataatctcatgac1560caaaatcccttaacgtgagttttcgttccactgagcgtcagaccccgtagaaaagatcaa1620aggatcttcttgagatcctttttttctgcgcgtaatctgctgcttgcaaacaaaaaaacc1680accgctaccagcggtggtttgtttgccggatcaagagctaccaactctttttccgaaggt1740aactggcttcagcagagcgcagataccaaatactgtccttctagtgtagccgtagttagg1800ccaccacttcaagaactctgtagcaccgcctacatacctcgctctgctaatcctgttacc1860agtggctgctgccagtggcgataagtcgtgtcttaccgggttggactcaagacgatagtt1920accggataaggcgcagcggtcgggctgaacggggggttcgtgcacacagcccagcttgga1980gcgaacgacctacaccgaactgagatacctacagcgtgagctatgagaaagcgccacgct2040tcccgaagggagaaaggcggacaggtatccggtaagcggcagggtcggaacaggagagcg2100cacgagggagcttccagggggaaacgcctggtatctttatagtcctgtcgggtttcgcca2160cctctgacttgagcgtcgatttttgtgatgctcgtcaggggggcggagcctatggaaaaa2220cgccagcaacgcggcctttttacggttcctggccttttgctggccttttgctcacatgtt2280ctttcctgcgttatcccctgattctgtggataaccgtattaccgcctttgagtgagctga2340taccgctcgccgcagccgaacgaccgagcgcagcgagtcagtgagcgaggaagcggaaga2400gcgcctgatgcggtattttctccttacgcatctgtgcggtatttcacaccgcatatatgg2460tgcactctcagtacaatctgctctgatgccgcatagttaagccagtatacactccgctat2520cgctacgtgactgggtcatggctgcgccccgacacccgccaacacccgctgacgcgccct2580gacgggcttgtctgctcccggcatccgcttacagacaagctgtgaccgtctccgggagct2640gcatgtgtcagaggttttcaccgtcatcaccgaaacgcgcgaggcagctgcggtaaagct2700catcagcgtggtcgtgaagcgattcacagatgtctgcctgttcatccgcgtccagctcgt2760tgagtttctccagaagcgttaatgtctggcttctgataaagcgggccatgttaagggcgg2820ttttttcctgtttggtcactgatgcctccgtgtaagggggatttctgttcatgggggtaa2880tgataccgatgaaacgagagaggatgctcacgatacgggttactgatgatgaacatgccc2940ggttactggaacgttgtgagggtaaacaactggcggtatggatgcggcgggaccagagaa3000aaatcactcagggtcaatgccagcgcttcgttaatacagatgtaggtgttccacagggta3060gccagcagcatcctgcgatgcagatccggaacataatggtgcagggcgctgacttccgcg3120tttccagactttacgaaacacggaaaccgaagaccattcatgttgttgctcaggtcgcag3180acgttttgcagcagcagtcgcttcacgttcgctcgcgtatcggtgattcattctgctaac3240cagtaaggcaaccccgccagcctagccgggtcctcaacgacaggagcacgatcatgcgca3300cccgtggggccgccatgccggcgataatggcctgcttctcgccgaaacgtttggtggcgg3360gaccagtgacgaaggcttgagcgagggcgtgcaagattccgaataccgcaagcgacaggc3420cgatcatcgtcgcgctccagcgaaagcggtcctcgccgaaaatgacccagagcgctgccg3480gcacctgtcctacgagttgcatgataaagaagacagtcataagtgcggcgacgatagtca3540tgccccgcgcccaccggaaggagctgactgggttgaaggctctcaagggcatcggtcgag3600atcccggtgcctaatgagtgagctaacttacattaattgcgttgcgctcactgcccgctt3660tccagtcgggaaacctgtcgtgccagctgcattaatgaatcggccaacgcgcggggagag3720gcggtttgcgtattgggcgccagggtggtttttcttttcaccagtgagacgggcaacagc3780tgattgcccttcaccgcctggccctgagagagttgcagcaagcggtccacgctggtttgc3840cccagcaggcgaaaatcctgtttgatggtggttaacggcgggatataacatgagctgtct3900tcggtatcgtcgtatcccactaccgagatatccgcaccaacgcgcagcccggactcggta3960atggcgcgcattgcgcccagcgccatctgatcgttggcaaccagcatcgcagtgggaacg4020atgccctcattcagcatttgcatggtttgttgaaaaccggacatggcactccagtcgcct4080tcccgttccgctatcggctgaatttgattgcgagtgagatatttatgccagccagccaga4140cgcagacgcgccgagacagaacttaatgggcccgctaacagcgcgatttgctggtgaccc4200aatgcgaccagatgctccacgcccagtcgcgtaccgtcttcatgggagaaaataatactg4260ttgatgggtgtctggtcagagacatcaagaaataacgccggaacattagtgcaggcagct4320tccacagcaatggcatcctggtcatccagcggatagttaatgatcagcccactgacgcgt4380tgcgcgagaagattgtgcaccgccgctttacaggcttcgacgccgcttcgttctaccatc4440gacaccaccacgctggcacccagttgatcggcgcgagatttaatcgccgcgacaatttgc4500gacggcgcgtgcagggccagactggaggtggcaacgccaatcagcaacgactgtttgccc4560gccagttgttgtgccacgcggttgggaatgtaattcagctccgccatcgccgcttccact4620ttttcccgcgttttcgcagaaacgtggctggcctggttcaccacgcgggaaacggtctga4680taagagacaccggcatactctgcgacatcgtataacgttactggtttcacattcaccacc4740ctgaattgactctcttccgggcgctatcatgccataccgcgaaaggttttgcgccattcg4800atggtgtccgggatctcgacgctctcccttatgcgactcctgcattaggaagcagcccag4860tagtaggttgaggccgttgagcaccgccgccgcaaggaatggtgcatgcaaggagatggc4920gcccaacagtcccccggccacggggcctgccaccatacccacgccgaaacaagcgctcat4980gagcccgaagtggcgagcccgatcttccccatcggtgatgtcggcgatataggcgccagc5040aaccgcacctgtggcgccggtgatgccggccacgatgcgtccggcgtagaggatcgagat5100ctcgatcccgcgaaattaatacgactcactataggggaattgtgagcggataacaattcc5160cctctagaaataattttgtttaactttaagaaggagatataccatgggcagcagccatca5220tcatcatcatcacagcagcggcctggtgccgcgcggcagccatatggaaaatctctactt5280ccaaggcgacaagaagtactccattgggctcgatatcggcacaaacagcgtcggctgggc5340cgtcattacggacgagtacaaggtgccgagcaaaaaattcaaagttctgggcaataccga5400tcgccacagcataaagaagaacctcattggcgccctcctgttcgactccggggagacggc5460cgaagccacgcggctcaaaagaacagcacggcgcagatatacccgcagaaagaatcggat5520ctgctacctgcaggagatctttagtaatgagatggctaaggtggatgactctttcttcca5580taggctggaggagtcctttttggtggaggaggataaaaagcacgagcgccacccaatctt5640tggcaatatcgtggacgaggtggcgtaccatgaaaagtacccaaccatatatcatctgag5700gaagaagcttgtagacagtactgataaggctgacttgcggttgatctatctcgcgctggc5760gcatatgatcaaatttcggggacacttcctcatcgagggggacctgaacccagacaacag5820cgatgtcgacaaactctttatccaactggttcagacttacaatcagcttttcgaagagaa5880cccgatcaacgcatccggagttgacgccaaagcaatcctgagcgctaggctgtccaaatc5940ccggcggctcgaaaacctcatcgcacagctccctggggagaagaagaacggcctgtttgg6000taatcttatcgccctgtcactcgggctgacccccaactttaaatctaacttcgacctggc6060cgaagatgccaagcttcaactgagcaaagacacctacgatgatgatctcgacaatctgct6120ggcccagatcggcgaccagtacgcagacctttttttggcggcaaagaacctgtcagacgc6180cattctgctgagtgatattctgcgagtgaacacggagatcaccaaagctccgctgagcgc6240tagtatgatcaagcgctatgatgagcaccaccaagacttgactttgctgaaggcccttgt6300cagacagcaactgcctgagaagtacaaggaaattttcttcgatcagtctaaaaatggcta6360cgccggatacattgacggcggagcaagccaggaggaattttacaaatttattaagcccat6420cttggaaaaaatggacggcaccgaggagctgctggtaaagcttaacagagaagatctgtt6480gcgcaaacagcgcactttcgacaatggaagcatcccccaccagattcacctgggcgaact6540gcacgctatcctcaggcggcaagaggatttctacccctttttgaaagataacagggaaaa6600gattgagaaaatcctcacatttcggataccctactatgtaggccccctcgcccggggaaa6660ttccagattcgcgtggatgactcgcaaatcagaagagaccatcactccctggaacttcga6720ggaagtcgtggataagggggcctctgcccagtccttcatcgaaaggatgactaactttga6780taaaaatctgcctaacgaaaaggtgcttcctaaacactctctgctgtacgagtacttcac6840agtttataacgagctcaccaaggtcaaatacgtcacagaagggatgagaaagccagcatt6900cctgtctggagagcagaagaaagctatcgtggacctcctcttcaagacgaaccggaaagt6960taccgtgaaacagctcaaagaagactatttcaaaaagattgaatgtttcgactctgttga7020aatcagcggagtggaggatcgcttcaacgcatccctgggaacgtatcacgatctcctgaa7080aatcattaaagacaaggacttcctggacaatgaggagaacgaggacattcttgaggacat7140tgtcctcacccttacgttgtttgaagatagggagatgattgaagaacgcttgaaaactta7200cgctcatctcttcgacgacaaagtcatgaaacagctcaagaggcgccgatatacaggatg7260ggggcggctgtcaagaaaactgatcaatgggatccgagacaagcagagtggaaagacaat7320cctggattttcttaagtccgatggatttgccaaccggaacttcatgcagttgatccatga7380tgactctctcacctttaaggaggacatccagaaagcacaagtttctggccagggggacag7440tcttcacgagcacatcgctaatcttgcaggtagcccagctatcaaaaagggaatactgca7500gaccgttaaggtcgtggatgaactcgtcaaagtaatgggaaggcataagcccgagaatat7560cgttatcgagatggcccgagagaaccaaactacccagaagggacagaagaacagtaggga7620aaggatgaagaggattgaagagggtataaaagaactggggtcccaaatccttaaggaaca7680cccagttgaaaacacccagcttcagaatgagaagctctacctgtactacctgcagaacgg7740cagggacatgtacgtggatcaggaactggacatcaatcggctctccgactacgacgtgga7800tcatatcgtgccccagtcttttctcaaagatgattctattgataataaagtgttgacaag7860atccgataaaaatagagggaagagtgataacgtcccctcagaagaagttgtcaagaaaat7920gaaaaattattggcggcagctgctgaacgccaaactgatcacacaacggaagttcgataa7980tctgactaaggctgaacgaggtggcctgtctgagttggataaagcaggcttcatcaaaag8040gcagcttgttgagacacgccagatcaccaagcacgtggcccaaattctcgattcacgcat8100gaacaccaagtacgatgaaaatgacaaactgattcgagaggtgaaagttattactctgaa8160gtctaagctggtctcagatttcagaaaggactttcagttttataaggtgagagagatcaa8220caattaccaccatgcgcatgatgcctacctgaatgcagtggtaggcactgcacttatcaa8280aaaatatcccaagcttgaatctgaatttgtttacggagactataaagtgtacgatgttag8340gaaaatgatcgcaaagtctgagcaggaaataggcaaggccaccgctaagtacttctttta8400cagcaatattatgaattttttcaagaccgagattacactggccaatggagagattcggaa8460gcgaccacttatcgaaacaaacggagaaacaggagaaatcgtgtgggacaagggtaggga8520tttcgcgacagtccggaaggtcctgtccatgccgcaggtgaacatcgttaaaaagaccga8580agtacagaccggaggcttctccaaggaaagtatcctcccgaaaaggaacagcgacaagct8640gatcgcacgcaaaaaagattgggaccccaagaaatacggcggattcgattctcctacagt8700cgcttacagtgtactggttgtggccaaagtggagaaagggaagtctaaaaaactcaaaag8760cgtcaaggaactgctgggcatcacaatcatggagcgatcaagcttcgaaaaaaaccccat8820cgactttctcgaggcgaaaggatataaagaggtcaaaaaagacctcatcattaagcttcc8880caagtactctctctttgagcttgaaaacggccggaaacgaatgctcgctagtgcgggcga8940gctgcagaaaggtaacgagctggcactgccctctaaatacgttaatttcttgtatctggc9000cagccactatgaaaagctcaaagggtctcccgaagataatgagcagaagcagctgttcgt9060ggaacaacacaaacactaccttgatgagatcatcgagcaaataagcgaattctccaaaag9120agtgatcctcgccgacgctaacctcgataaggtgctttctgcttacaataagcacaggga9180taagcccatcagggagcaggcagaaaacattatccacttgtttactctgaccaacttggg9240cgcgcctgcagccttcaagtacttcgacaccaccatagacagaaagcggtacacctctac9300aaaggaggtcctggacgccacactgattcatcagtcaattacggggctctatgaaacaag9360aatcgacctctctcagctcggtggagactgactcgagcaccaccaccaccaccactgaga9420tccggctgctaacaaagcccgaaaggaagctgagttggctgctgccaccgctgagcaata9480actagcataaccccttggggcctctaaacgggtcttgaggggttttttgctgaaaggagg9540aactatatccgga9553<210>9<211>9553<212>dna<213>人工序列(artificialsequence)<400>9tggcgaatgggacgcgccctgtagcggcgcattaagcgcggcgggtgtggtggttacgcg60cagcgtgaccgctacacttgccagcgccctagcgcccgctcctttcgctttcttcccttc120ctttctcgccacgttcgccggctttccccgtcaagctctaaatcgggggctccctttagg180gttccgatttagtgctttacggcacctcgaccccaaaaaacttgattagggtgatggttc240acgtagtgggccatcgccctgatagacggtttttcgccctttgacgttggagtccacgtt300ctttaatagtggactcttgttccaaactggaacaacactcaaccctatctcggtctattc360ttttgatttataagggattttgccgatttcggcctattggttaaaaaatgagctgattta420acaaaaatttaacgcgaattttaacaaaatattaacgtttacaatttcaggtggcacttt480tcggggaaatgtgcgcggaacccctatttgtttatttttctaaatacattcaaatatgta540tccgctcatgagacaataaccctgataaatgcttcaataatattgaaaaaggaagagtat600gagtattcaacatttccgtgtcgcccttattcccttttttgcggcattttgccttcctgt660ttttgctcacccagaaacgctggtgaaagtaaaagatgctgaagatcagttgggtgcacg720agtgggttacatcgaactggatctcaacagcggtaagatccttgagagttttcgccccga780agaacgttttccaatgatgagcacttttaaagttctgctatgtggcgcggtattatcccg840tattgacgccgggcaagagcaactcggtcgccgcatacactattctcagaatgacttggt900tgagtactcaccagtcacagaaaagcatcttacggatggcatgacagtaagagaattatg960cagtgctgccataaccatgagtgataacactgcggccaacttacttctgacaacgatcgg1020aggaccgaaggagctaaccgcttttttgcacaacatgggggatcatgtaactcgccttga1080tcgttgggaaccggagctgaatgaagccataccaaacgacgagcgtgacaccacgatgcc1140tgcagcaatggcaacaacgttgcgcaaactattaactggcgaactacttactctagcttc1200ccggcaacaattaatagactggatggaggcggataaagttgcaggaccacttctgcgctc1260ggcccttccggctggctggtttattgctgataaatctggagccggtgagcgtgggtctcg1320cggtatcattgcagcactggggccagatggtaagccctcccgtatcgtagttatctacac1380gacggggagtcaggcaactatggatgaacgaaatagacagatcgctgagataggtgcctc1440actgattaagcattggtaactgtcagaccaagtttactcatatatactttagattgattt1500aaaacttcatttttaatttaaaaggatctaggtgaagatcctttttgataatctcatgac1560caaaatcccttaacgtgagttttcgttccactgagcgtcagaccccgtagaaaagatcaa1620aggatcttcttgagatcctttttttctgcgcgtaatctgctgcttgcaaacaaaaaaacc1680accgctaccagcggtggtttgtttgccggatcaagagctaccaactctttttccgaaggt1740aactggcttcagcagagcgcagataccaaatactgtccttctagtgtagccgtagttagg1800ccaccacttcaagaactctgtagcaccgcctacatacctcgctctgctaatcctgttacc1860agtggctgctgccagtggcgataagtcgtgtcttaccgggttggactcaagacgatagtt1920accggataaggcgcagcggtcgggctgaacggggggttcgtgcacacagcccagcttgga1980gcgaacgacctacaccgaactgagatacctacagcgtgagctatgagaaagcgccacgct2040tcccgaagggagaaaggcggacaggtatccggtaagcggcagggtcggaacaggagagcg2100cacgagggagcttccagggggaaacgcctggtatctttatagtcctgtcgggtttcgcca2160cctctgacttgagcgtcgatttttgtgatgctcgtcaggggggcggagcctatggaaaaa2220cgccagcaacgcggcctttttacggttcctggccttttgctggccttttgctcacatgtt2280ctttcctgcgttatcccctgattctgtggataaccgtattaccgcctttgagtgagctga2340taccgctcgccgcagccgaacgaccgagcgcagcgagtcagtgagcgaggaagcggaaga2400gcgcctgatgcggtattttctccttacgcatctgtgcggtatttcacaccgcatatatgg2460tgcactctcagtacaatctgctctgatgccgcatagttaagccagtatacactccgctat2520cgctacgtgactgggtcatggctgcgccccgacacccgccaacacccgctgacgcgccct2580gacgggcttgtctgctcccggcatccgcttacagacaagctgtgaccgtctccgggagct2640gcatgtgtcagaggttttcaccgtcatcaccgaaacgcgcgaggcagctgcggtaaagct2700catcagcgtggtcgtgaagcgattcacagatgtctgcctgttcatccgcgtccagctcgt2760tgagtttctccagaagcgttaatgtctggcttctgataaagcgggccatgttaagggcgg2820ttttttcctgtttggtcactgatgcctccgtgtaagggggatttctgttcatgggggtaa2880tgataccgatgaaacgagagaggatgctcacgatacgggttactgatgatgaacatgccc2940ggttactggaacgttgtgagggtaaacaactggcggtatggatgcggcgggaccagagaa3000aaatcactcagggtcaatgccagcgcttcgttaatacagatgtaggtgttccacagggta3060gccagcagcatcctgcgatgcagatccggaacataatggtgcagggcgctgacttccgcg3120tttccagactttacgaaacacggaaaccgaagaccattcatgttgttgctcaggtcgcag3180acgttttgcagcagcagtcgcttcacgttcgctcgcgtatcggtgattcattctgctaac3240cagtaaggcaaccccgccagcctagccgggtcctcaacgacaggagcacgatcatgcgca3300cccgtggggccgccatgccggcgataatggcctgcttctcgccgaaacgtttggtggcgg3360gaccagtgacgaaggcttgagcgagggcgtgcaagattccgaataccgcaagcgacaggc3420cgatcatcgtcgcgctccagcgaaagcggtcctcgccgaaaatgacccagagcgctgccg3480gcacctgtcctacgagttgcatgataaagaagacagtcataagtgcggcgacgatagtca3540tgccccgcgcccaccggaaggagctgactgggttgaaggctctcaagggcatcggtcgag3600atcccggtgcctaatgagtgagctaacttacattaattgcgttgcgctcactgcccgctt3660tccagtcgggaaacctgtcgtgccagctgcattaatgaatcggccaacgcgcggggagag3720gcggtttgcgtattgggcgccagggtggtttttcttttcaccagtgagacgggcaacagc3780tgattgcccttcaccgcctggccctgagagagttgcagcaagcggtccacgctggtttgc3840cccagcaggcgaaaatcctgtttgatggtggttaacggcgggatataacatgagctgtct3900tcggtatcgtcgtatcccactaccgagatatccgcaccaacgcgcagcccggactcggta3960atggcgcgcattgcgcccagcgccatctgatcgttggcaaccagcatcgcagtgggaacg4020atgccctcattcagcatttgcatggtttgttgaaaaccggacatggcactccagtcgcct4080tcccgttccgctatcggctgaatttgattgcgagtgagatatttatgccagccagccaga4140cgcagacgcgccgagacagaacttaatgggcccgctaacagcgcgatttgctggtgaccc4200aatgcgaccagatgctccacgcccagtcgcgtaccgtcttcatgggagaaaataatactg4260ttgatgggtgtctggtcagagacatcaagaaataacgccggaacattagtgcaggcagct4320tccacagcaatggcatcctggtcatccagcggatagttaatgatcagcccactgacgcgt4380tgcgcgagaagattgtgcaccgccgctttacaggcttcgacgccgcttcgttctaccatc4440gacaccaccacgctggcacccagttgatcggcgcgagatttaatcgccgcgacaatttgc4500gacggcgcgtgcagggccagactggaggtggcaacgccaatcagcaacgactgtttgccc4560gccagttgttgtgccacgcggttgggaatgtaattcagctccgccatcgccgcttccact4620ttttcccgcgttttcgcagaaacgtggctggcctggttcaccacgcgggaaacggtctga4680taagagacaccggcatactctgcgacatcgtataacgttactggtttcacattcaccacc4740ctgaattgactctcttccgggcgctatcatgccataccgcgaaaggttttgcgccattcg4800atggtgtccgggatctcgacgctctcccttatgcgactcctgcattaggaagcagcccag4860tagtaggttgaggccgttgagcaccgccgccgcaaggaatggtgcatgcaaggagatggc4920gcccaacagtcccccggccacggggcctgccaccatacccacgccgaaacaagcgctcat4980gagcccgaagtggcgagcccgatcttccccatcggtgatgtcggcgatataggcgccagc5040aaccgcacctgtggcgccggtgatgccggccacgatgcgtccggcgtagaggatcgagat5100ctcgatcccgcgaaattaatacgactcactataggggaattgtgagcggataacaattcc5160cctctagaaataattttgtttaactttaagaaggagatataccatgggcagcagccatca5220tcatcatcatcacagcagcggcctggtgccgcgcggcagccatatggaaaatctctactt5280ccaaggcgacaagaagtactccattgggctcgatatcggcacaaacagcgtcggctgggc5340cgtcattacggacgagtacaaggtgccgagcaaaaaattcaaagttctgggcaataccga5400tcgccacagcataaagaagaacctcattggcgccctcctgttcgactccggggagacggc5460cgaagccacgcggctcaaaagaacagcacggcgcagatatacccgcagaaagaatcggat5520ctgctacctgcaggagatctttagtaatgagatggctaaggtggatgactctttcttcca5580taggctggaggagtcctttttggtggaggaggataaaaagcacgagcgccacccaatctt5640tggcaatatcgtggacgaggtggcgtaccatgaaaagtacccaaccatatatcatctgag5700gaagaagcttgtagacagtactgataaggctgacttgcggttgatctatctcgcgctggc5760gcatatgatcaaatttcggggacacttcctcatcgagggggacctgaacccagacaacag5820cgatgtcgacaaactctttatccaactggttcagacttacaatcagcttttcgaagagaa5880cccgatcaacgcatccggagttgacgccaaagcaatcctgagcgctaggctgtccaaatc5940ccggcggctcgaaaacctcatcgcacagctccctggggagaagaagaacggcctgtttgg6000taatcttatcgccctgtcactcgggctgacccccaactttaaatctaacttcgacctggc6060cgaagataccaagcttcaactgagcaaagacacctacgatgatgatctcgacaatctgct6120ggcccagatcggcgaccagtacgcagacctttttttggcggcaaagaacctgtcagacgc6180cattctgctgagtgatattctgcgagtgaacacggagatcaccaaagctccgctgagcgc6240tagtatgatcaagctgtatgatgagcaccaccaagacttgactttgctgaaggcccttgt6300cagacagcaactgcctgagaagtacaaggaaattttcttcgatcagtctaaaaatggcta6360cgccggatacattgacggcggagcaagccaggaggaattttacaaatttattaagcccat6420cttggaaaaaatggacggcaccgaggagctgctggtaaagcttaacagagaagatctgtt6480gcgcaaacagcgcactttcgacaatggaattatcccccaccagattcacctgggcgaact6540gcacgctatcctcaggcggcaagaggatttctacccctttttgaaagataacagggaaaa6600gattgagaaaatcctcacatttcggataccctactatgtaggccccctcgcccggggaaa6660ttccagattcgcgtggatgactcgcaaatcagaagagaccatcactccctggaacttcga6720gaaagtcgtggataagggggcctctgcccagtccttcatcgaaaggatgactaactttga6780taaaaatctgcctaacgaaaaggtgcttcctaaacactctctgctgtacgagtacttcac6840agtttataacgagctcaccaaggtcaaatacgtcacagaagggatgagaaagccagcatt6900cctgtctggagatcagaagaaagctatcgtggacctcctcttcaagacgaaccggaaagt6960taccgtgaaacagctcaaagaagactatttcaaaaagattgaatgtttcgactctgttga7020aatcagcggagtggaggatcgcttcaacgcatccctgggaacgtatcacgatctcctgaa7080aatcattaaagacaaggacttcctggacaatgaggagaacgaggacattcttgaggacat7140tgtcctcacccttacgttgtttgaagatagggagatgattgaagaacgcttgaaaactta7200cgctcatctcttcgacgacaaagtcatgaaacagctcaagaggcgccgatatacaggatg7260ggggcggctgtcaagaaaactgatcaatgggatccgagacaagcagagtggaaagacaat7320cctggattttcttaagtccgatggatttgccaaccggaacttcattcagttgatccatga7380tgactctctcacctttaaggaggacatccagaaagcacaagtttctggccagggggacag7440tcttcacgagcacatcgctaatcttgcaggtagcccagctatcaaaaagggaatactgca7500gaccgttaaggtcgtggatgaactcgtcaaagtaatgggaaggcataagcccgagaatat7560cgttatcgagatggcccgagagaaccaaactacccagaagggacagaagaacagtaggga7620aaggatgaagaggattgaagagggtataaaagaactggggtcccaaatccttaaggaaca7680cccagttgaaaacacccagcttcagaatgagaagctctacctgtactacctgcagaacgg7740cagggacatgtacgtggatcaggaactggacatcaatcggctctccgactacgacgtgga7800tcatatcgtgccccagtcttttctcaaagatgattctattgataataaagtgttgacaag7860atccgataaaaatagagggaagagtgataacgtcccctcagaagaagttgtcaagaaaat7920gaaaaattattggcggcagctgctgaacgccaaactgatcacacaacggaagttcgataa7980tctgactaaggctgaacgaggtggcctgtctgagttggataaagcaggcttcatcaaaag8040gcagcttgttgagacacgccagatcaccaagcacgtggcccaaattctcgattcacgcat8100gaacaccaagtacgatgaaaatgacaaactgattcgagaggtgaaagttattactctgaa8160gtctaagctggtctcagatttcagaaaggactttcagttttataaggtgagagagatcaa8220caattaccaccatgcgcatgatgcctacctgaatgcagtggtaggcactgcacttatcaa8280aaaatatcccaagcttgaatctgaatttgtttacggagactataaagtgtacgatgttag8340gaaaatgatcgcaaagtctgagcaggaaataggcaaggccaccgctaagtacttctttta8400cagcaatattatgaattttttcaagaccgagattacactggccaatggagagattcggaa8460gcgaccacttatcgaaacaaacggagaaacaggagaaatcgtgtgggacaagggtaggga8520tttcgcgacagtccggaaggtcctgtccatgccgcaggtgaacatcgttaaaaagaccga8580agtacagaccggaggcttctccaaggaaagtatcctcccgaaaaggaacagcgacaagct8640gatcgcacgcaaaaaagattgggaccccaagaaatacggcggattcgattctcctacagt8700cgcttacagtgtactggttgtggccaaagtggagaaagggaagtctaaaaaactcaaaag8760cgtcaaggaactgctgggcatcacaatcatggagcgatcaagcttcgaaaaaaaccccat8820cgactttctcgaggcgaaaggatataaagaggtcaaaaaagacctcatcattaagcttcc8880caagtactctctctttgagcttgaaaacggccggaaacgaatgctcgctagtgcgggcgt8940tctgcagaaaggtaacgagctggcactgccctctaaatacgttaatttcttgtatctggc9000cagccactatgaaaagctcaaagggtctcccgaagataatgagcagaagcagctgttcgt9060ggaacaacacaaacactaccttgatgagatcatcgagcaaataagcgaattctccaaaag9120agtgatcctcgccgacgctaacctcgataaggtgctttctgcttacaataagcacaggga9180taagcccatcagggagcaggcagaaaacattatccacttgtttactctgaccaacttggg9240cgcgcctgcagccttcaagtacttcgacaccaccatagacagaaagcggtacacctctac9300aaaggaggtcctggacgccacactgattcatcagtcaattacggggctctatgaaacaag9360aatcgacctctctcagctcggtggagactgactcgagcaccaccaccaccaccactgaga9420tccggctgctaacaaagcccgaaaggaagctgagttggctgctgccaccgctgagcaata9480actagcataaccccttggggcctctaaacgggtcttgaggggttttttgctgaaaggagg9540aactatatccgga9553<210>10<211>920<212>dna<213>人工序列(artificialsequence)<400>10tagtcctgtcgggtttcgccacctctgacttgagcgtcgatttttgtgatgctcgtcagg60ggggcggagcctatggaaaaacgccagcaacgcggcctttttacggttcctggccttttg120ctggccttttgctcacatgttctttcctgcgttatcccctgattctgtggataaccgtat180taccgcctttgagtgagctgataccgctcgccgcagccgaacgaccgagcgcagcgagtc240agtgagcgaggaagcggaagagcgcccaatacgcaaaccgcctctccccgcgcgttggcc300gattcattaatgcagctggcacgacaggtttcccgactggaaagcgggcagtgagcgcaa360cgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttcc420ggctcgtatgttgtgtggaattgtgagcggataacaatttcacacaggaaacagctatga480ccatgattacgccaagctcgaaattaaccctcactaaagggaacaaaagctggagctcca540ccgcggtggcggccgctctagaactagtggatcccccgggctgcaggaattcgatatcaa600gcttatcgattaccgctccagtcgttcatgtggttagagctagaaatagcaagttaaaat660aaggctagtccgttatcaacttgaaaaagtggcaccgagtcggtgctctcgagggggggc720ccggtacccaattcgccctatagtgagtcgtattacaattcactggccgtcgttttacaa780cgtcgtgactgggaaaaccctggcgttacccaacttaatcgccttgcagcacatccccct840ttcgccagctggcgtaatagcgaagaggcccgcaccgatcgcccttcccaacagttgcgc900agcctgaatggcgaatggaa920<210>11<211>97<212>dna<213>人工序列(artificialsequence)<400>11taccgctccagtcgttcatggttttagagctagaaatagcaagttaaaataaggctagtc60cgttatcaacttgaaaaagtggcaccgagtcggtgct97<210>12<211>20<212>dna<213>人工序列(artificialsequence)<400>12taccgctccagtcgttcatg20当前第1页12当前第1页12