全人源的新冠IgG1单链抗体及其应用的制作方法

文档序号:26228907发布日期:2021-08-10 16:28阅读:860来源:国知局
全人源的新冠IgG1单链抗体及其应用的制作方法

本发明涉及生物医药技术领域,具体涉及全人源的新冠igg1单链抗体及其应用。



背景技术:

新型冠状病毒是一种具有包膜的、不分节段的正链单股rna病毒,颗粒呈圆形或椭圆形,直径约80~120nm,属于网巢病毒目冠状病毒科betacoronavirus。病毒粒子被宿主细胞提供的脂质双层所包裹,其中含有核酸及核衣壳蛋白,有三种主要蛋白:包膜蛋白(e蛋白)、膜蛋白(m蛋白)和刺突蛋白(s蛋白)。此病毒每组基因组长度约三万个核苷酸左右,基因序列显示sars-cov-2属于乙型冠状病毒属谱系β(betacoronaviruslineageβ,sarbecovirus)进化树中分支较长的一种病毒,与中华菊头蝠中发现的冠状病毒相似,例如mers-cov或sars-cov。对病毒的生物遗传学分析显示,同属人类冠状病毒的sars病毒分离株ay274119相比mers病毒分离株kc164505、jx869059等在亲缘关系上距离sars-cov-2病毒更近。

2019年末,该病爆发,其后向全球扩散。病毒潜伏期平均大约3-7天,最长不超过14天。大多数患者的表现以下呼吸道症状为主,常见临床表现包括发热、四肢乏力、干咳等症状,其他表现包含鼻塞、流鼻涕、头痛、咽痛、咳血,咳痰、或腹泻等。有部分患者仅表现为低热、轻微乏力等,无肺炎表现。还有部分患者无任何临床表现。病毒感染严重后,会引发多种并发症包含急性呼吸窘迫综合征(ards)、脓毒症休克、全身炎症反应综合征、难以纠正的代谢性酸中毒、急性心肌损伤,和出凝血功能障碍等。

针对这场危及全人类的群体大流行,揭示人体对新冠侵染后的免疫过程,获取新冠病毒的b细胞受体对于新冠特异性药物的筛选、病毒疫苗的生产研发及其重要。b细胞受体(bcellreceptor,bcr)是b细胞抗原识别决定性表面分子,其本质是一种膜表面免疫球蛋白(membraneimmunoglobulin,mig)。bcr具有抗原结合特异性,每个个体的bcr多样性高达5×10^13,构成容量巨大的bcr库,赋予个体识别各种抗原、产生特异性抗体的巨大潜能。

bcr的结构包括重链和轻链。bcr的重链(heavychain,h)由65~100种可变区(vh)、2种多变区(dh)、6种结合区(jh)和恒定区(ch)四部分基因片段组成;轻链(lightchain,l)由可变区、结合区和恒定区三部分基因片段组成。发育过程中的b细胞在重组酶(rag1、rag2)作用下,形成了多样性高达1~2×10^11的bcr。同时,由其形成互补决定区(complementaritiesdeterminingregion,cdr):cdr1、cdr2和cdr3区氨基酸序列的多样性,特别是编码cdr3的基因,由于其位于轻链v、j或重链v、d、j片段的连接处,可以通过v(d)j的重排和(或)两个基因片段的连接间丢失或插入数个核苷酸,进一步增加bcr的多样性,而形成具有功能的bcr编码基因(b细胞克隆)。

bcr测序是通过高通量测序技术检测靶向扩增后的bcr重链和轻链,全面解析bcr基因重排碱基序列,以及各序列的丰度的测序技术。bcr测序常用于评价各种免疫相关疾病和遗传性突变引起的某个物种所有b细胞或特定b细胞激活介导的细胞免疫反应中bcr基因重排碱基序列,以及各序列的丰度,用于研究不同b细胞克隆的转录情况和相互间关系,从而揭示更深层次的b细胞功能特异性,继而解释体液免疫应答耐受以及高频突变在b细胞应答识别抗原异常等相关生命现象。传统的bcr测序都是采用使用illumina的测序仪进行2×300bp或2×150bp的双端测序方法来对bcr进行测序,该方法的测序准确率高,但对于部分长度超过600bp的bcr序列,该方法仅能获得两端的序列,会存在中间部分关键可变区序列缺失的问题。

pacbio公司正式推出sequelii新一代测序系统后,依赖于pacbiosmrt测序技术独有的ccs测序模式,能够通过滚环测序提升reads准确度,同时结合聚合酶试剂的优化,酶读长大幅提升,在获得高精准度hifireads的同时能够保证10kb以上的插入片段读长,将解决原有的illumina等二代测序平台下无法完整覆盖整个片段区域的问题。通过进行hifi测序,可以获得准确性达到99.5%以上的长读长序列。

目前,针对新冠病毒侵染人体后引起的bcr免疫反应,常规的方法是采用二代测序平台对抗体的可变区进行测序后筛选新冠相关抗体,但是受限于测序的读长,仅能够获得可变区的部分序列,无法进行准确的抗体类型识别与编码翻译识别,而基于三代测序平台pacbio的新冠病毒bcr全长扩增测序可轻松读取病毒侵染后的bcr全长序列,突破了二代测序读长较短的局限性,提高了针对新冠病毒特异性bcr抗体的分辨能力,极大的提高了新冠特异性bcr抗体鉴定的分辨率与准确率。特别是在pacbio公司正式推出sequelii新一代测序系统后,依赖于pacbiosmrt测序技术独有的hifi测序模式,能够通过滚环测序提升reads准确度,目前采用hifi技术进行bcr抗体全长测序的准确率可以达到99.5%以上,通过进行hifi测序,可以获得极高准确性的新冠bcr抗体全长序列,用于新冠相关bcr抗体序列筛选、基于全长抗体序列的表达翻译与后续新冠中和反应评估等。

bcr一般包括与膜结合的免疫球蛋白分子和ig-α/ig-β信号转导组件,通过二硫键连接着免疫球蛋白分子和信号转导组件。bcr包括以下两部分:1.某一亚型(igd、igm、iga、igg或者ige)的膜结合免疫球蛋白(mig)。除了c端疏水的膜结合区和胞内区,这些膜结合免疫球蛋白和分泌型的免疫球蛋白单体是相同的,有两条重链(ighs)和两条轻链(igls);2.信号转导组件:ig-α/ig-β的异二聚体(cd79),由二硫键连接。两个亚基都是跨膜蛋白,在胞内区都有免疫受体酪氨酸的活化基序(itam)。

免疫球蛋白igg是人血清和细胞外液中含量最高的一类免疫球蛋白,约占血清总免疫球蛋白的75%~80%,是分子质量最小的一类免疫球蛋白,具有典型的免疫球蛋白单体结构。在人体中,igg于出生后3个月开始合成,3~5岁接近成人水平。igg由浆细胞合成,骨髓造血干细胞最初分化为前b细胞,进一步发育成未成熟的b细胞,此时可在细胞表面表达igm,此后相继表达igd并继续发育。只有能表达igm且不能识别自身抗原的未成熟b细胞才能继续发育成熟成为具有免疫功能的b细胞,成熟的b细胞经外周血迁出,进入脾脏、淋巴结。b细胞在遇到抗原刺激后会分化增殖为浆母细胞,根据产生抗体时是否需要t细胞,可将b细胞分为b1和b2两个亚群,其中b2为t细胞依赖性细胞,这类细胞在受胸腺依赖性抗原刺激后发生免疫应答。igg即由b2分化成的浆细胞产生,根据它们在血清中的含量,igg有4个亚类,命名为igg1、igg2、igg3和igg4,并且各自都有着不同的功能。

目前为止,未能通过筛选方式获得新冠特异性的igg1bcr抗体全长。



技术实现要素:

本发明目的在于提供一种全人源的新冠igg1单链抗体,这类特异识别新冠的单链抗体本身或者其可变区序列,经过直接表达或经基因工程改造成其它的抗体形式后,可以作为抗新冠药物开发、疫苗生产、检测标志物开发等进行新冠相关应用中。

本发明提供一种全人源的新冠igg1单链抗体,包括如seqidno:18-seqidno:34中至少一种所示的氨基酸序列。

本发明还提供,编码上述新冠igg1单链抗体的基因序列,优选地,包括如seqidno:1-seqidno:17中至少一种与上述氨基酸对应的核苷酸序列。

本发明还提供,包含上述新冠igg1单链抗体中所述基因序列的文库。

本发明还提供,上述文库的制备方法,对不同新冠康复者共有而在正常人群中没有的bcr序列进行分析并筛选出新冠特异性的igg1bcr抗体序列。

进一步地,所述分析筛选过程为:对新冠康复者与正常人群进行bcr全长测序,将测序的原始数据进行hifi的一致性校正后,获得了质量值在q20以上的bcr全长一致性序列,经过与bcr数据库中的抗体恒定区序列进行比对后,获得了每个样本测序数据中的不同类别的bcr抗体序列。

本发明还提供,包含上述新冠igg1单链抗体中所述基因序列的表达载体。

本发明还提供,包含上述新冠igg1单链抗体中所述基因序列的宿主细胞。

本发明还提供上述新冠igg1单链抗体在制备新冠病毒治疗药物、药物载体及检测标记物中的应用。

相比现有技术,本发明的有益效果为:

1.本发明通过使用bcr全长扩增子测序的方法对新冠康复者与健康人群进行了bcr全长扩增建库测序,获得了质量值在q20以上的bcr全长扩增子序列,获得的bcr序列包括完整的启动子至终止密码子的的区域,所获得的序列为全人源,无需进一步进行整合即可进行后续的表达验证。

2.本发明通过pacbiohifi测序获得高质量的bcr序列,经与数据库进行比对后,根据人的bcr序列在恒定区的保守性,对获得的bcr全长序列进行转录方向校正与分类,构建新冠康复者与建库人群不同类别的bcr抗体全长数据库;传统的二代bcr测序技术仅进行部分可变区的测序,无法准确进行抗体的聚类筛选。

3.本发明针对获取的不同类型的bcr序列,基于不同类别抗体恒定区bcr序列的一致性,通过定位位于恒定区上的终止密码子位置,将获得的抗体直接翻译为蛋白质氨基酸序列,在蛋白质水平上对抗体进行比较;传统的二代bcr测序方法不涉及bcr序列的恒定区,无法进行准确的翻译,仅能在dna序列的水平上进行比较。

4.本发明通过对新冠康复者与正常人群的不同类别的抗体蛋白质氨基酸序列进行比较,找到新冠康复者共有而在正常人群中没有的bcr抗体,这些抗体为新冠特异性抗体;其抗体dna序列为全人源的新冠特异性抗体全长序列,在经过直接表达或经基因工程改造成其它的抗体形式后,可以作为抗新冠药物开发、疫苗生产、检测标志物开发等进行新冠相关应用中;传统的新冠相关应用均是基于可变区的部分序列进行基因工程改造后再进行表达,由于其序列并非全人源,且必须经过改造,可能引入某些未知的安全问题。

5.本发明通过对获取的新冠特异性抗体序列设计特异性引物,该引物扩增区域包括启动子至终止密码子的全部区域,使用该引物在新冠康复者的cdna作为模板进行pcr扩增后,将扩增产物转移至大肠杆菌表达载体中,通过构建单克隆后进行一代测序,基于扩增片段大小筛选获取的目标序列进行测序,在测序数据中找到了单一的新冠特异性的抗体dna序列,获取的dna序列为单一的纯的全人源特定抗体dna序列,无需进行人工合成即可直接作为抗新冠药物开发、疫苗生产、检测标志物开发的原始dna反应物。

附图说明

为了更清楚地说明本发明实施例的技术方案,下面将对实施例中所需要使用的附图作简单地介绍,应当理解,以下附图仅示出了本发明的某些实施例,因此不应被看作是对范围的限定,对于本领域普通技术人员来讲,在不付出创造性劳动的前提下,还可以根据这些附图获得其他相关的附图。

图1是本发明新冠相关特异性抗体筛选与获取原理示意图。

图2是本发明特异性引物扩增富集新冠相关bcr序列电泳图示意图。

图3是本发明单克隆制备培养示意图。

图4是本发明单克隆扩增筛选电泳图。

图5是本发明单克隆一代测序峰图。

具体实施方式

以下实例用于说明本发明,但不限制本发明的范围。在不背离本发明精神和实质的前提下,对本发明的方法、步骤或条件所作的修改或替换,均属于本发明的范围。

如图1所示,本发明提供的全人源的新冠单链抗体获取流程如下:

1.通过进行新冠康复者与健康人群的bcr全长扩增子建库测序,获取质量值q20以上的bcr全长序列,分别构建新冠康复者与健康人群的bcr抗体库;

2..在获得高质量的bcr抗体序列后,与抗体数据库进行比对,构建bcr抗体恒定区序列确定抗体类别,由于同类型的bcr抗体相似性非常高,且进行翻译时翻译的终止密码子位置也是一致,基于终止密码子位置将bcr抗体序列翻译为蛋白质氨基酸序列;

3.通过比较新冠康复者共有,而健康人群没有的bcr蛋白质氨基酸序列,找到新冠特异性相关的不同类别的bcr序列;

4.基于找到的新冠特异性序列设计特异引物,进行扩增特异性富集新冠相关的抗体序列;

5.将抗体序列转移至感受态细胞中,制备单克隆,挑选单克隆进行扩增后,选择特定片段大小的扩增产物进行一代测序,基于一代测序的结果筛选获得的全人源的新冠相关的bcr序列。

在本发明中,获得了新冠特异性igg1序列17条,获得的新冠特异性igg1序列如seqidno:1-seqidno:17所示,经翻译后的氨基酸序列如seqidno:18-seqidno:34所示。所有的序列均包含vdj区域,在5’端包括起始密码子atg,在3’端包括终止密码子tga,该抗体序列为全人源序列,可安全的应用于后续疫苗生产与抗体药物研发等应用中。

根据本发明的新冠特异性igg1中特异性核苷酸或氨基酸序列,可在体外人工合成与此相同的抗体轻重链基因的核苷酸序列或编码相同氨基酸的核苷酸序列,从而获得相同的抗体基因或用于相关基因的改造,而获得igg1抗体或相关蛋白。

在本发明中,提出的包含上述新冠igg1单链抗体中所述基因序列的文库,所述文库为:通过对新冠康复者与健康人群的bcr全长扩增子建库测序,获取质量值q20以上的bcr全长序列,分别构建新冠康复者与健康人群的bcr抗体库;经与数据库进行比对后,根据人的bcr序列在恒定区的保守性,对获得的bcr全长序列进行转录方向校正与分类,构建新冠康复者与建库人群不同类别的bcr抗体全长数据库。

在本发明中,提出包含所述基因序列的表达载体,根据本领域的常识,载体可以为原核细胞表达载体、真核细胞表达载体或昆虫细胞表达载体。

在本发明中,提出包含所述表达载体的宿主细胞,根据本领域的常识,所述的宿主细胞可以为原核表达细胞、真核表达细胞或昆虫细胞,所述原核表达细胞优选大肠杆菌。

实施例一:新冠康复者与正常人群bcr全长扩增测序

新冠康复者与正常人群的bcr全长扩增测序参照前期申请的专利方法(公开号cn111662970a)进行,主要流程如下:

1.全血样本totalrna的提取

取新鲜全血样本1ml,使用trizolls进行全血样本totalrna提取,提取后使用nanidrop2000c测定rna样本的浓度与纯度,使用agilent2100测定样本的完整性,达到合格标准的样本(总量>1μg,完整性rin值>7)的样本进行后续实验。

2.总rna中cdna第一链的合成

实验操作流程如下:

1)oligodt逆转录引物与poly(a)结合,如表1所示。

表1:

轻弹混匀,瞬时离心,70℃孵育5min后立即置于冰上。

2)逆转录合成cdna第一链,配制表2中的反应。

表2:

轻弹混匀,瞬时离心,42℃孵育75min。反应完成后立即置于冰上,加入1ul的bcrtemplateswitchingoligo,轻弹混匀,瞬时离心,42℃孵育15min。

3.bcrcdna的全长扩增

bcrcdna的全长扩增包括两轮半巢式扩增反应,第一轮扩增进行bcr序列的初步富集,第二轮扩增时采用内部的巢式引物,进一步提高扩增的特异性,使扩增条带单一。

1)bcrcdna全长的第一轮pcr扩增

取一新的0.2mlpcr管,加入表3中的试剂。

表3:

充分混匀,瞬时离心,置于pcr仪上进行pcr反应:98℃2min;98℃20s、65℃15s、72℃45s,18cycles;72℃5min。

2)bcrcdna全长的第二轮pcr扩增

取一新的0.2mlpcr管,加入表4中的试剂。

表4:

10组混合引物均需要单独进行扩增,可以提高扩增反应的稳定性。

充分混匀,瞬时离心,置于pcr仪上进行pcr反应:98℃2min;98℃20s、65℃15s、72℃30s,20cycles;72℃5min。

反应结束后按照ampure磁珠的使用说明对扩增产物进行磁珠纯化,最后使用10μl洗脱缓冲液洗脱,取1μl纯化产物,使用无核酸酶水稀释5倍后进行qubit定量。

4.bcr全长扩增子片段混样

根据qubit定量结果,将同一样本的不同扩增产物进行等量混样,要求混样后的总量在1μg以上,用于后续建库。

5.文库构建

1)末端修复

取1μg的全基因组扩增子样本,进行末端修复反应体系的配制,配制表5中的反应。

表5:

充分混匀,瞬时离心,20℃孵育30min。

反应完成后,按照ampure磁珠的使用说明进行1x磁珠纯化,去除反应时加入的酶与buffer,最后使用14μl洗脱缓冲液洗脱,获得片段末端加a的粘性末端。

2)连接带barcode的测序接头

通过末端修复与加a后,在加入具有与a末端匹配的带barcode的测序接头,在连接酶的作用下即可实现接头的连接。反应体系如表6。

表6:

混匀,瞬时离心,20℃孵育60min,反应结束后65℃孵育10min,置于冰上。进行外切酶消化,反应体系如表7。

表7:

混匀,瞬时离心,37℃孵育60min,置于冰上。按照ampure磁珠的使用说明进行磁珠纯化,最后使用20μl洗脱缓冲液洗脱,获得的适用于pacbio测序平台的哑铃型的环状文库。

3)文库质检与上机测序

取1μl文库进行qubit定量,获得文库浓度;取1μl文库进行安捷伦2100的片段大小分析,将全长扩增文库在pacbiosequelii测序平台上进行混合测序,每个样本获得了约60g的测序数据。

实施例二:新冠康复者与正常人群bcr免疫组库分析,获得新冠特异性igg1单链抗体

将测序的原始数据进行hifi的一致性校正后,获得了质量值在q20以上的bcr全长一致性序列,经过与bcr数据库中的抗体恒定区序列进行比对后,获得了每个样本测序数据中的不同类别的bcr抗体序列,同时基于恒定区的终止密码子位置,将dna序列翻译为蛋白质多肽序列。通过比较新冠康复者共有的,而正常人群中没有的bcr多肽序列,获得了新冠特异性相关的抗体全长。本次获得的新冠特异性igg1序列17条,所有的序列均包含vdj区域,在5’端包括起始密码子atg,在3’端包括终止密码子tga,该抗体序列为全人源序列,可安全的应用于后续疫苗生产与抗体药物研发等应用中。获得的新冠特异性igg1序列如seqidno:1-seqidno:17所示,经翻译后的氨基酸序列如seqidno:18-seqidno:34所示。

实施例三:基于以新冠康复者样本设计特异性引物,进行单克隆bcr抗体库的构建

基于抗体库筛选的igg1抗体序列,在起始密码子的与终止密码子的外测设计特异性引物,通过pcr扩增特异性富集目标抗体序列。由于抗体存在多重重组的特性,获取的扩增产物是一类与引物匹配的抗体序列集合,无法直接进行一代测序与后续应用,需要将抗体序列进行克隆实验,获取单个抗体序列的单克隆,并挑选单克隆进行测序,验证所获得的单克隆序列为目标抗体。具体流程如下:

1.目标抗体igg1抗体序列引物设计

基于测序筛选的目标序列抗体设计引物,该引物扩增的目标片段包括起始密码子与终止密码子区域,覆盖整个抗体表达区域的全长,包含实施例二公开抗体的所有序列,具体设计的引物序列如表8所示。

表8:igg1抗体序列引物

其中,primer_id_01~03为r端通用引物,该引物在恒定区内,所有igg1抗体序列均可与该引物进行匹配,使用时混合在一起作为r端通用引物使用;primer_id_04~20为f端引物,该引物序列基于筛选的新冠相关特异性抗体设计,与r端引物组合在一起为一对引物,用于目标抗体序列的扩增。

2.扩增富集获取新冠相关特异性抗体

本步骤使用的扩增模板为新冠康复者样本的单链cdna样本。取一新的0.2mlpcr管,加入如表9所示试剂。

表9:

充分混匀,瞬时离心,置于pcr仪上进行pcr反应:98℃2min;98℃20s、65℃15s、72℃120s,35cycles;72℃5min。

反应结束后进行电泳检测,电泳结果示意图如图2所示。切胶获取片段大小在1.5~2k的目标特异性片段。

3.目标片段的单克隆制备

本步骤使用表10中的试剂。

表10:

lb固体培养基配制:取本品8克,溶解于250ml蒸馏水中,121℃高压灭菌15分钟,至不烫手时加入250ul氨苄青霉素,混匀倒平板备用(每个平板15ml左右)。

lb液体培养基配制:取本品1克,溶解于40ml蒸馏水中,121℃高压灭菌15分钟,分装至2ml无菌离心管中备用。

配制好培养基后进行载体连接反应,配制如表11中的反应体系。

表11:

轻弹管底混匀,低速瞬时离心收集所有液体于离心管底,室温25℃反应5min。反应结束后,将离心管置于冰上。

将fast-t1感受态细胞从-70℃拿出,迅速置于冰上融化,取20ul感受态细胞加入目的载体连接反应产物,轻弹管壁混匀(避免用枪吸打),冰上静置30min。

42℃水浴热激30s后,迅速置于冰上静置2min,切勿摇动离心管。

向离心管中加入200μllb液体培养基(不含抗生素),混匀后置于37℃,200rpm摇床中复苏5min。

复苏后颠倒混匀直接取200μl,涂布在含氨苄青霉素的lb固体培养基平板上,将平板正置于37℃培养箱10min,待菌液被完全吸收后,倒置平板,过夜培养,单克隆制备培养结果示意图如图3所示。

4.单克隆筛选与测序鉴定

在平板培养基上挑选单克隆菌落至10μlddh2o中混匀作为模板;使用2×rapidtaqmastermix(vazyme#p222)进行扩增,反应体系如表12所示。

表12:

放置在pcr仪上进行如表13所示的扩增反应程序。

表13:

将扩增获得的扩增产物进行凝胶电泳检测,获得图4所示的电泳图,挑选扩增条带亮度高、扩增条带单一且片段大小在1.5~5k的扩增产物进行双端单克隆一代测序鉴定,测序峰图如图5所示,根据一代测序的序列与目标抗体序列进行一致性比对,选择序列完全一致的单克隆进一步进行扩增放大,即获得了高纯度的单一的新冠相关抗体特异性序列,该序列可以直接转移至假病毒体系中,进行后续的抗体效价验证,也可以直接转移至抗体表达体系中进行表达。

序列表

<110>武汉菲沙基因组医学有限公司

<120>全人源的新冠igg1单链抗体及其应用

<160>54

<170>siposequencelisting1.0

<210>1

<211>1404

<212>dna

<213>igg1

<400>1

atggacacactttgctccacgctcctgctgctgaccatcccttcatgggtcttgtcccag60

atcaccttgaaggagtctggtcctacgctggtgagacccacacagaccctcacgctgacc120

tgcaccttctctgggttctcactcagcactactggagtgggtgtgggctggatccgtcag180

cccccaggaaaggccctggagtggctaacactcatttattgggatgatgataagcgttac240

aggccatctctgcagagcaggctcaccatcaccaaggacacctccaaaaaccaggtggtc300

cttacaatgaccaacatggaccctgtggacacaggcacatattattgtgcacacgttcat360

ctcagtggcctgggatactggggccagggagccctggtcaccgtctcctcagcctccacc420

aagggcccatcggtcttccccctggcaccctcctccaagagcacctctgggggcacagcg480

gccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactca540

ggcgccctgaccagcggcgtgcacaccttcccggctgtcctacagtcctcaggactctac600

tccctcagcagcgtggtgaccgtgccctccagcagcttgggcacccagacctacatctgc660

aacgtgaatcacaagcccagcaacaccaaggtggacaagaaagttgagcccaaatcttgt720

gacaaaactcacacatgcccaccgtgcccagcacctgaactcctggggggaccgtcagtc780

ttcctcttccccccaaaacccaaggacaccctcatgatctcccggacccctgaggtcaca840

tgcgtggtggtggacgtgagccacgaagaccctgaggtcaagttcaactggtacgtggac900

ggcgtggaggtgcataatgccaagacaaagccgcgggaggagcagtacaacagcacgtac960

cgtgtggtcagcgtcctcaccgtcctgcaccaggactggctgaatggcaaggagtacaag1020

tgcaaggtctccaacaaagccctcccagcccccatcgagaaaaccatctccaaagccaaa1080

gggcagccccgagaaccacaggtgtacaccctgcccccatcccgggaggagatgaccaag1140

aaccaggtcagcctgacctgcctggtcaaaggcttctaccccagcgacatcgccgtggag1200

tgggagagcaatgggcagccggagaacaactacaagaccacacctcccatgctggactcc1260

gacggctccttcttcctctacagcaagctcaccgtggacaagagcaggtggcagcagggg1320

aacgtcttctcatgctccgtgatgcatgaggctctgcacaaccactacacgcagaagagc1380

ctctccctgtctccgggtaaatga1404

<210>2

<211>1443

<212>dna

<213>igg1

<400>2

atggacctcctgcacaagaacatgaaacacctgtggttcttcctcctcctggtggcagct60

cccagatgggtcctgtcccaggtgcaggtacaacagtggggcgcaggactgttgaagcct120

tcggagaccctgtccctcacctgcgctgtctatggtgggtccttcagtggttactattgg180

agctggatccgccagcccccagggaaggggctggagtggattggggaaatcaatcatagt240

ggaagcaccaactacaacccgtccctcaagagtcgagtcagcatatcagtagacacgtcc300

aagaaccagttctccctgaagctgagctctgttaccgccgcggacacggctgtgttttac360

tgtgcgagaggccggtatagcagcacctggtacggaggtcgggggtggttcgacccctgg420

ggccagggaaccctggtcaccgtctcctcagcctccaccaagggcccatcggtcttcccc480

ctggcaccctcctccaagagcacctctgggggcacagcagccctgggctgcctggtcaag540

gactacttccccgaaccggtgacggtgtcgtggaactcaggcgccctgaccagcggcgtg600

cacaccttcccggctgtcctacagtcctcaggactctactccctcagcagcgtggtgacc660

gtgccctccagcagcttgggcacccagacctacatctgcaacgtgaatcacaagcccagc720

aacaccaaggtggacaagaaagttgagcccaaatcttgtgacaaaactcacacatgccca780

ccgtgcccagcacctgaactcctggggggaccgtcagtcttcctcttccccccaaaaccc840

aaggacaccctcatgatctcccggacccctgaggtcacatgcgtggtggtggacgtgagc900

cacgaagaccctgaggtcaagttcaactggtacgtggacggcgtggaggtgcataatgcc960

aagacaaagccgcgggaggagcagtacaacagcacgtaccgtgtggtcagcgtcctcacc1020

gtcctgcaccaggactggctgaatggcaaggagtacaagtgcaaggtctccaacaaagcc1080

ctcccagcccccatcgagaaaaccatctccaaagccaaagggcagccccgagaaccacag1140

gtgtacaccctgcccccatcccgggatgagctgaccaagaaccaggtcagcctgacctgc1200

ctggtcaaaggcttctatcccagcgacatcgccgtggagtgggagagcaatgggcagccg1260

gagaacaactacaagaccacgcctcccgtgctggactccgacggctccttcttcctctac1320

agcaagctcaccgtggacaagagcaggtggcagcaggggaacgtcttctcatgctccgtg1380

atgcatgaggctctgcacaaccactacacacagaagagcctctccctgtctccgggtaaa1440

tga1443

<210>3

<211>1428

<212>dna

<213>igg1

<400>3

atggactggacctggagcatccttttcttggtggcagcagcaacaggtgcccactcccag60

ggtttcctggtgcagtctggagctgaggtgaagaaacctggggcctcattgaaagtctcc120

tgcaaggcttctggttacacctttacccactacggtatcaactgggtgcgacaggcccct180

ggacaggggcttgagtggatgggatggatcagcggttacaatggtaatacacactatgca240

cagaagttccagggcagactcaccatgaccacagacacatccacgagcacagcctacatg300

gagctgaggggcctgagatctgacgacacagccgtgtattactgtgcgagagatcttttc360

gtaacccgagattactatgggtcggggactggttatggtgactggggccagggaaccctg420

gtcaccgtctcctcagcctccaccaagggcccatcggtcttccccctggcaccctcctcc480

aagagcacctctgggggcacagcggccctgggctgcctggtcaaggactacttccccgaa540

ccggtgacggtgtcgtggaactcaggcgccctgaccagcggcgtgcacaccttcccggct600

gtcctacagtcctcaggactctactccctcagcagcgtggtgaccgtgccctccagcagc660

ttgggcacccagacctacatctgcaacgtgaatcacaagcccagcaacaccaaggtggac720

aagaaagttgagcccaaatcttgtgacaaaactcacacatgcccaccgtgcccagcacct780

gaactcctggggggaccgtcagtcttcctcttccccccaaaacccaaggacaccctcatg840

atctcccggacccctgaggtcacatgcgtggtggtggacgtgagccacgaagaccctgag900

gtcaagttcaactggtacgtggacggcgtggaggtgcataatgccaagacaaagccgcgg960

gaggagcagtacaacagcacgtaccgtgtggtcagcgtcctcaccgtcctgcaccaggac1020

tggctgaatggcaaggagtacaagtgcaaggtctccaacaaagccctcccagcccccatc1080

gagaaaaccatctccaaagccaaagggcagccccgagaaccacaggtgtacaccctgccc1140

ccatcccgggatgagctgaccaagaaccaggtcagcctgacctgcctggtcaaaggcttc1200

tatcccagcgacatcgccgtggagtgggagagcaatgggcagccggagaacaactacaag1260

accacgcctcccgtgctggactccgacggctccttcttcctctacagcaagctcaccgtg1320

gacaagagcaggtggcagcaggggaacgtcttctcatgctccgtgatgcatgaggctctg1380

cacaaccactacacgcagaagagcctctccctgtctccgggtaaatga1428

<210>4

<211>1416

<212>dna

<213>igg1

<400>4

atggactggacctggaggttcctcttcgtggtggcagcagctacaggtgtccagtcccag60

gtccacctggtgcagtctggggctgaggtgaagaagcctgggtcctcggtgaaggtctcc120

tgcaaggcttctggaggcaccttcgacaccaatgctatcaactgggtgcgacaggtccct180

gggctagggcttgagtggatgggaaggatcatccctgtccttggaacagtgaactccgca240

cacaacttccagggcagagtcacaataaccgcggacaaatccacgaacacagcctacatg300

gaggtgagcagcctgagatccgaggacacggccgtctattactgtgcgagagatcggggg360

actactggctactattactacatggacctctggggcaaagggaccacggtcaccgtctcc420

tcagcctccaccaagggcccatcggtcttccccctggcaccctcctccaagagcacctct480

gggggcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtg540

tcgtggaactcaggcgccctgaccagcggcgtgcacaccttcccggctgtcctacagtcc600

tcaggactctactccctcagcagcgtggtgaccgtgccctccagcagcttgggcacccag660

acctacatctgcaacgtgaatcacaagcccagcaacaccaaggtggacaagagagttgag720

cccaaatcttgtgacaaaactcacacatgcccaccgtgcccagcacctgaactcctgggg780

ggaccgtcagtcttcctcttccccccaaaacccaaggacaccctcatgatctcccggacc840

cctgaggtcacatgcgtggtggtggacgtgagccacgaagaccctgaggtcaagttcaac900

tggtacgtggacggcgtggaggtgcataatgccaagacaaagccgcgggaggagcagtac960

aacagcacgtaccgtgtggtcagcgtcctcaccgtcctgcaccaggactggctgaatggc1020

aaggagtacaagtgcaaggtctccaacaaagccctcccagcccccatcgagaaaaccatc1080

tccaaagccaaagggcagccccgagaaccacaggtgtacaccctgcccccatcccgggat1140

gagctgaccaagaaccaggtcagcctgacctgcctggtcaaaggcttctatcccagcgac1200

atcgccgtggagtgggagagcaatgggcagccggagaacaactacaagaccacgcctccc1260

gtgctggactccgacggctccttcttcctctacagcaagctcaccgtggacaagagcagg1320

tggcagcaggggaacgtcttctcatgctccgtgatgcatgaggctctgcacaaccactac1380

acgcagaagagcctctccctgtctccgggtaaatga1416

<210>5

<211>1413

<212>dna

<213>igg1

<400>5

atggactggacctggaggttcctctttgtggtggcagcagcaacaggtgtccagtcccag60

gtgcggctggtgcagtctggggctgaggtgaagaagcctgggtcctcggtgaaggtctcc120

tgcaaggcttctggaggcaccttcagcacctttgctatcagctgggtgcgacaggcccct180

ggacaagggcttgagtggatgggagggatcatccctatcttaggtacagcatactactca240

cagaacttccagggcagagtcaccattaccgcggacgaatccacgagcacagcccacatg300

gagctgagcagcctgatatctgaggacacggccgtgtattactgtgcgagagcttggagt360

tcctactactactacgctatggacgtctggggccaagggaccacggtcaccgtctcctca420

gcctccaccaagggcccatcggtcttccccctggcaccctcctccaagagcacctctggg480

ggcacagcagccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcg540

tggaactcaggcgccctgaccagcggcgtgcacaccttcccggctgtcctacagtcctca600

ggactctactccctcagcagcgtggtgaccgtgccctccagcagcttgggcacccagacc660

tacatctgcaacgtgaatcacaagcccagcaacaccaaggtggacaagaaagttgagccc720

aaatcttgtgacaaaactcacacatgcccaccgtgcccagcacctgaactcctgggggga780

ccgtcagtcttcctcttccccccaaaacccaaggacaccctcatgatctcccggacccct840

gaggtcacatgcgtggtggtggacgtgagccacgaagaccctgaggtcaagttcaactgg900

tacgtggacggcgtggaggtgcataatgccaagacaaagccgcgggaggagcagtacaac960

agcacgtaccgtgtggtcagcgtcctcaccgtcctgcaccaggactggctgaatggcaag1020

gagtacaagtgcaaggtctccaacaaagccctcccagcccccatcgagaaaaccatctcc1080

aaagccaaagggcagccccgagaaccacaggtgtacaccctgcccccatcccgggatgag1140

ctgaccaagaaccaggtcagcctgacctgcctggtcaaaggcttctatcccagcgacatc1200

gccgtggagtgggagagcaatgggcagccggagaacaactacaagaccacgcctcccgtg1260

ctggactccgacggctccttcttcctctacagcaagctcaccgtggacaagagcaggtgg1320

cagcaggggaacgtcttctcatgctccgtgatgcatgaggctctgcacaaccactacaca1380

cagaagagcctctccctgtctccgggtaaatga1413

<210>6

<211>1413

<212>dna

<213>igg1

<400>6

atggagtttgggctgagctgggttttcctcgttgctcttttaagaggtgtccagtgtcag60

gtgcagctggtggagtctgggggaggcgtggtccagcctgggaggtccctgagactctcc120

tgtgcagcctctggattcaccttcagtagctatgctatgcactgggtccgccaggctcca180

ggcaaggggctggagtgggtggcagttatatcatatgatggaagcaataaatactacgca240

gactccgtgaagggccgattcaccatatccagagacaattccaagaacacgctgtatctg300

caaatgaacagcctgagagctgaggacacggctgtgtattactgtgcgaggggaagaacg360

acaggttactactacggtatggacgtctggggccaagggaccacggtcaccgtctcctca420

gcctccaccaagggcccatcggtcttccccctggcaccctcctccaagagcacctctggg480

ggcacagcagccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcg540

tggaactcaggcgccctgaccagcggcgtgcacaccttcccggctgtcctacagtcctca600

ggactctactccctcagcagcgtggtgaccgtgccctccagcagcttgggcacccagacc660

tacatctgcaacgtgaatcacaagcccagcaacaccaaggtggacaagaaagttgagccc720

aaatcttgtgacaaaactcacacatgcccaccgtgcccagcacctgaactcctgggggga780

ccgtcagtcttcctcttccccccaaaacccaaggacaccctcatgatctcccggacccct840

gaggtcacatgcgtggtggtggacgtgagccacgaagaccctgaggtcaagttcaactgg900

tacgtggacggcgtggaggtgcataatgccaagacaaagccgcgggaggagcagtacaac960

agcacgtaccgtgtggtcagcgtcctcaccgtcctgcaccaggactggctgaatggcaag1020

gagtacaagtgcaaggtctccaacaaagccctcccagcccccatcgagaaaaccatctcc1080

aaagccaaagggcagccccgagaaccacaggtgtacaccctgcccccatcccgggatgag1140

ctgaccaagaaccaggtcagcctgacctgcctggtcaaaggcttctatcccagcgacatc1200

gccgtggagtgggagagcaatgggcagccggagaacaactacaagaccacgcctcccgtg1260

ctggactccgacggctccttcttcctctacagcaagctcaccgtggacaagagcaggtgg1320

cagcaggggaacgtcttctcatgctccgtgatgcatgaggctctgcacaaccactacaca1380

cagaagagcctctccctgtctccgggtaaatga1413

<210>7

<211>1413

<212>dna

<213>igg1

<400>7

atggagtttgggctgagctgggttttcctcgttgctcttttaagaggtgtccagtgtcag60

gtgcagctggtggagtctgggggaggcgtggtccagcctgggaggtccctgagactctcc120

tgtgcagcctctggattcaccttcagtagctatgctctgcactgggtccgccaggctcca180

ggcaagggactggagtgggtggcagttatatcatatgatggaagcaataaatattacgca240

gactccgtgaagggccgattcaccatctccagagacaattccaagaacacgctgtatctg300

cagatgaacagcctgagagctgaggacacggctgtgtattactgtgcgagaagctcttcg360

gggagttattatcactactttgactactggggccagggaaccctggtcaccgtctcctca420

gcctccaccaagggcccatcggtcttccccctggcaccctcctccaagagcacctctggg480

ggcacagcagccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcg540

tggaactcaggcgccctgaccagcggcgtgcacaccttcccggctgtcctacagtcctca600

ggactctactccctcagcagcgtggtgaccgtgccctccagcagcttgggcacccagacc660

tacatctgcaacgtgaatcacaagcccagcaacaccaaggtggacaagaaagttgagccc720

aaatcttgtgacaaaactcacacatgcccaccgtgcccagcacctgaactcctgggggga780

ccgtcagtcttcctcttccccccaaaacccaaggacaccctcatgatctcccggacccct840

gaggtcacatgcgtggtggtggacgtgagccacgaagaccctgaggtcaagttcaactgg900

tacgtggacggcgtggaggtgcataatgccaagacaaagccgcgggaggagcagtacaac960

agcacgtaccgtgtggtcagcgtcctcaccgtcctgcaccaggactggctgaatggcaag1020

gagtacaagtgcaaggtctccaacaaagccctcccagcccccatcgagaaaaccatctcc1080

aaagccaaagggcagccccgagaaccacaggtgtacaccctgcccccatcccgggatgag1140

ctgaccaagaaccaggtcagcctgacctgcctggtcaaaggcttctatcccagcgacatc1200

gccgtggagtgggagagcaatgggcagccggagaacaactacaagaccacgcctcccgtg1260

ctggactccgacggctccttcttcctctacagcaagctcaccgtggacaagagcaggtgg1320

cagcaggggaacgtcttctcatgctccgtgatgcatgaggctctgcacaaccactacaca1380

cagaagagcctctccctgtctccgggtaaatga1413

<210>8

<211>1413

<212>dna

<213>igg1

<400>8

atggagtttgggctgagctgggttttcctcgttgctcttttaagaggtgtccagtgtcag60

gtgcagctggtggagtctgggggaggcgtggtccggcctgggaggtccctgagactctcc120

tgtgcagcctctggattcaccttcagtagctatgctctgcactgggtccgccaggctcca180

ggcaagggactggagtgggtggcagttatatcatatgatggaagcaataaatattacgca240

gactccgtgaagggccgattcaccatctccagagacaattccaagaacacgctgtatctg300

cagatgaacagcctgagagctgaggacacggctgtgtattactgtgcgagaagctcttcg360

gggagttattatcactactttgactactggggccagggaaccctggtcaccgtctcctca420

gcctccaccaagggcccatcggtcttccccctggcaccctcctccaagagcacctctggg480

ggcacagcggccctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcg540

tggaactcaggcgccctgaccagcggcgtgcacaccttcccggctgtcctacagtcctca600

ggactctactccctcagcagcgtggtgaccgtgccctccagcagcttgggcacccagacc660

tacatctgcaacgtgaatcacaagcccagcaacaccaaggtggacaagaaagttgagccc720

aaatcttgtgacaaaactcacacatgcccaccgtgcccagcacctgaactcctgggggga780

ccgtcagtcttcctcttccccccaaaacccaaggacaccctcatgatctcccggacccct840

gaggtcacatgcgtggtggtggacgtgagccacgaagaccctgaggtcaagttcaactgg900

tacgtggacggcgtggaggtgcataatgccaagacaaagccgcgggaggagcagtacaac960

agcacgtaccgtgtggtcagcgtcctcaccgtcctgcaccaggactggctgaatggcaag1020

gagtacaagtgcaaggtctccaacaaagccctcccagcccccatcgagaaaaccatctcc1080

aaagccaaagggcagccccgagaaccacaggtgtacaccctgcccccatcccgggatgag1140

ctgaccaagaaccaggtcagcctgacctgcctggtcaaaggcttctatcccagcgacatc1200

gccgtggagtgggagagcaatgggcagccggagaacaactacaagaccacgcctcccgtg1260

ctggactccgacggctccttcttcctctacagcaagctcaccgtggacaagagcaggtgg1320

cagcaggggaacgtcttctcatgctccgtgatgcatgaggctctgcacaaccactacacg1380

cagaagagcctctccctgtctccgggtaaatga1413

<210>9

<211>1434

<212>dna

<213>igg1

<400>9

atggactggacctggaggttcctctttgtggtggcagcagcgacaggtgtccagtcccag60

gtgcagctggtgcagtctggggctgaggtgaagaggcctgggtcctcggtgaaggtctcc120

tgcaaggcttctggaggcaccttcagcagctatgctatcagctgggtgcgacaggcccct180

ggacaagggcttgagtggatgggagggatcatcccaatgtttggtacagcaaaccacgca240

cagaagttccagggcagaatcacgattaccgcgggcgaatccacgaatacagcctacatg300

gagctgagcagcctgagatctgaggacacggccgtgtattactgtgcgagaggggtttat360

tgtggtggtgactgcccgactcggtactactactacggtatggacgtctggggccaaggg420

accacggtcaccgtctcctcagcctccaccaagggcccatcggtcttccccctggcaccc480

tcctccaagagcacctctgggggcacagcggccctgggctgcctggtcaaggactacttc540

cccgaaccggtgacggtgtcgtggaactcaggcgccctgaccagcggcgtgcacaccttc600

ccggctgtcctacagtcctcaggactctactccctcagcagcgtggtgaccgtgccctcc660

agcagcttgggcacccagacctacatctgcaacgtgaatcacaagcccagcaacaccaag720

gtggacaagaaagttgagcccaaatcttgtgacaaaactcacacatgcccaccgtgccca780

gcacctgaactcctggggggaccgtcagtcttcctcttccccccaaaacccaaggacacc840

ctcatgatctcccggacccctgaggtcacatgcgtggtggtggacgtgagccacgaagac900

cctgaggtcaagttcaactggtacgtggacggcgtggaggtgcataatgccaagacaaag960

ccgcgggaggagcagtacaacagcacgtaccgtgtggtcagcgtcctcaccgtcctgcac1020

caggactggctgaatggcaaggagtacaagtgcaaggtctccaacaaagccctcccagcc1080

cccatcgagaaaaccatctccaaagccaaagggcagccccgagaaccacaggtgtacacc1140

ctgcccccatcccgggatgagctgaccaagaaccaggtcagcctgacctgcctggtcaaa1200

ggcttctatcccagcgacatcgccgtggagtgggagagcaatgggcagccggagaacaac1260

tacaagaccacgcctcccatgctggactccgacggctccttcttcctctacagcaagctc1320

accgtggacaagagcaggtggcagcaggggaacgtcttctcatgctccgtgatgcatgag1380

gctctgcacaaccactacacgcagaagagcctctccctgtctccgggtaaatga1434

<210>10

<211>1446

<212>dna

<213>igg1

<400>10

atggagttttggctgagctgggttttccttgttgctattttaaaaggtgtccagtgtgag60

atgcacctggtggagtctggaggaggcttggtccagccgggggggtccctgagactctcc120

tgtgcagtctctgggttcaccgtcagtagcaaccacttgaactgggtccgccaggctcca180

gggaaggggctggagtgggtctcagtcatttatagcggtggtagcacatactacgcagac240

tccgtggagggccgattcaccatctcaagagacaattccaagaatacgctgtatcttcaa300

atgaacagtttgagaggtgaagacacggccgtgtattattgtgcgagagaaaattcggtg360

gcttggttcggggagttattatttaatcggggagagaattactattacgggatggacgtc420

tggggccaagggaccacggtcaccgtctcctcagcctccaccaagggcccatcggtcttc480

cccctggcaccctcctccaagagcacctctgggggcacagcggccctgggctgcctggtc540

aaggactacttccccgaaccggtgacggtgtcgtggaactcaggcgccctgaccagcggc600

gtgcacaccttcccggctgtcctacagtcctcaggactctactccctcagcagcgtggtg660

accgtgccctccagcagcttgggcacccagacctacatctgcaacgtgaatcacaagccc720

agcaacaccaaggtggacaagagagttgagcccaaatcttgtgacaaaactcacacatgc780

ccaccgtgcccagcacctgaactcctggggggaccgtcagtcttcctcttccccccaaaa840

cccaaggacaccctcatgatctcccggacccctgaggtcacatgcgtggtggtggacgtg900

agccacgaagaccctgaggtcaagttcaactggtacgtggacggcgtggaggtgcataat960

gccaagacaaagccgcgggaggagcagtacaacagcacgtaccgtgtggtcagcgtcctc1020

accgtcctgcaccaggactggctgaatggcaaggagtacaagtgcaaggtctccaacaaa1080

gccctcccagcccccatcgagaaaaccatctccaaagccaaagggcagccccgagaacca1140

caggtgtacaccctgcccccatcccgggatgagctgaccaagaaccaggtcagcctgacc1200

tgcctggtcaaaggcttctatcccagcgacatcgccgtggagtgggagagcaatgggcag1260

ccggagaacaactacaagaccacgcctcccgtgctggactccgacggctccttcttcctc1320

tacagcaagctcaccgtggacaagagcaggtggcagcaggggaacgtcttctcatgctcc1380

gtgatgcatgaggctctgcacaaccactacacgcagaagagcctctccctgtctccgggt1440

aaatga1446

<210>11

<211>1401

<212>dna

<213>igg1

<400>11

atggagttggggctgagctgggttttccttgttgctatattagaaggtgtccagtgtgag60

gtgcagctggtggagtctgggggaggcttggtacagcctggggggtccctgagactctcc120

tgtgcagcctctggattcaccttcagtagctacgacatgcactgggtccgccaagctaca180

ggaaaaggtctggaatgggtctcagctattggtactgctggtgacacatactatccaggc240

tccgtgaagggccgattcaccatctccagagaaaatgccaagaactccttgtatcttcaa300

atgaacagcctgagagccggggacacggctgtgtattactgtgcaagagatctttcttac360

tacggtatggacgtctggggccaagggaccacggtcaccgtctcctcagcctccaccaag420

ggcccatcggtcttccccctggcaccctcctccaagagcacctctgggggcacagcggcc480

ctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactcaggc540

gccctgaccagcggcgtgcacaccttcccggctgtcctacagtcctcaggactctactcc600

ctcagcagcgtggtgaccgtgccctccagcagcttgggcacccagacctacatctgcaac660

gtgaatcacaagcccagcaacaccaaggtggacaagaaagttgagcccaaatcttgtgac720

aaaactcacacatgcccaccgtgcccagcacctgaactcctggggggaccgtcagtcttc780

ctcttccccccaaaacccaaggacaccctcatgatctcccggacccctgaggtcacatgc840

gtggtggtggacgtgagccacgaagaccctgaggtcaagttcaactggtacgtggacggc900

gtggaggtgcataatgccaagacaaagccgcgggaggagcagtacaacagcacgtaccgt960

gtggtcagcgtcctcaccgtcctgcaccaggactggctgaatggcaaggagtacaagtgc1020

aaggtctccaacaaagccctcccagcccccatcgagaaaaccatctccaaagccaaaggg1080

cagccccgagaaccacaggtgtacaccctgcccccatcccgggatgagctgaccaagaac1140

caggtcagcctgacctgcctggtcaaaggcttctatcccagcgacatcgccgtggagtgg1200

gagagcaatgggcagccggagaacaactacaagaccacgcctcccgtgctggactccgac1260

ggctccttcttcctctacagcaagctcaccgtggacaagagcaggtggcagcaggggaac1320

gtcttctcatgctccgtgatgcatgagggtctgcacaaccactacacgcagaagagcctc1380

tccctgtctccgggtaaatga1401

<210>12

<211>1401

<212>dna

<213>igg1

<400>12

atggagttttggctgagctgggttttccttgttgctattttaaaaggtgtccagtgtgag60

gtgcagctggtggagtctggaggaggcttggtccagcctggggggtccctgagactctcc120

tgtgcagcctctgggttcaccgtcagtagcaactacatgagctgggtccgccaggctcca180

gggaaggggctggagtgggtctcagttatttatagcggtggtagcacatactacgcagac240

tccgtgaagggccgattcaccatctccagacacaattccaagaacacgctgtatcttcaa300

atgaacagcctgagagctgaggacacggccgtgtattactgtgcgagagatctgagtgac360

tacggtatggacgtctggggccaagggaccacggtcaccgtctcctcagcctccaccaag420

ggcccatcggtcttccccctggcaccctcctccaagagcacctctgggggcacagcggcc480

ctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactcaggc540

gccctgaccagcggcgtgcacaccttcccggctgtcctacagtcctcaggactctactcc600

ctcagcagcgtggtgaccgtgccctccagcagcttgggcacccagacctacatctgcaac660

gtgaatcacaagcccagcaacaccaaggtggacaagaaagttgagcccaaatcttgtgac720

aaaactcacacatgcccaccgtgcccagcacctgaactcctggggggaccgtcagtcttc780

ctcttccccccaaaacccaaggacaccctcatgatctcccggacccctgaggtcacatgc840

gtggtggtggacgtgagccacgaagaccctgaggtcaagttcaactggtacgtggacggc900

gtggaggtgcataatgccaagacaaagccgcgggaggagcagtacaacagcacgtaccgt960

gtggtcagcgtcctcaccgtcctgcaccaggactggctgaatggcaaggagtacaagtgc1020

aaggtctccaacaaagccctcccagcccccatcgagaaaaccatctccaaagccaaaggg1080

cagccccgagaaccacaggtgtacaccctgcccccatcccgggatgagctgaccaagaac1140

caggtcagcctgacctgcctggtcaaaggcttctatcccagcgacatcgccgtggagtgg1200

gagagcaatgggcagccggagaacaactacaagaccacgcctcccgtgctggactccgac1260

ggctccttcttcctctacagcaagctcaccgtggacaagagcaggtggcagcaggggaac1320

gtcttctcatgctccgtgatgcatgagggtctgcacaaccactacacgcagaagagcctc1380

tccctgtctccgggtaaatga1401

<210>13

<211>1401

<212>dna

<213>igg1

<400>13

atggagttttggctgagctgggttttccttgttgctattttaaaaggtgtccagtgtgag60

gtgcagctggtggagtctggaggaggcttggtccagcctggggggtccctgagactctcc120

tgtgcagcctctgggttcaccgtcagtagcaactacatgagttgggtccgccaggctcca180

gggaaggggctggagtgggtctcagttatttatagcggtggtagcacatactacgcagac240

tccgtgaagggccgattcaccatctccagacacaattccaagaacacgctgtatcttcaa300

atgaacagcctgagagctgaggacacggccgtgtattactgtgcgagagatcttatcgtc360

tacggtatggacgtctggggccaagggaccacggtcaccgtctcctcagcctccaccaag420

ggcccatcggtcttccccctggcaccctcctccaagagcacctctgggggcacagcggcc480

ctgggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactcaggc540

gccctgaccagcggcgtgcacaccttcccggctgtcctacagtcctcaggactctactcc600

ctcagcagcgtggtgaccgtgccctccagcagcttgggcacccagacctacatctgcaac660

gtgaatcacaagcccagcaacaccaaggtggacaagaaagttgagcccaaatcttgtgac720

aaaactcacacatgcccaccgtgcccagcacctgaactcctggggggaccgtcagtcttc780

ctcttccccccaaaacccaaggacaccctcatgatctcccggacccctgaggtcacatgc840

gtggtggtggacgtgagccacgaagaccctgaggtcaagttcaactggtacgtggacggc900

gtggaggtgcataatgccaagacaaagccgcgggaggagcagtacaacagcacgtaccgt960

gtggtcagcgtcctcaccgtcctgcaccaggactggctgaatggcaaggagtacaagtgc1020

aaggtctccaacaaagccctcccagcccccatcgagaaaaccatctccaaagccaaaggg1080

cagccccgagaaccacaggtgtacaccctgcccccatcccgggatgagctgaccaagaac1140

caggtcagcctgacctgcctggtcaaaggcttctatcccagcgacatcgccgtggagtgg1200

gagagcaatgggcagccggagaacaactacaagaccacgcctcccgtgctggactccgac1260

ggctccttcttcctctacagcaagctcaccgtggacaagagcaggtggcagcaggggaac1320

gtcttctcatgctccgtgatgcatgagggtctgcacaaccactacacgcagaagagcctc1380

tccctgtctccgggtaaatga1401

<210>14

<211>1389

<212>dna

<213>igg1

<400>14

atggagtttgggctgagctgggttttcctcgttgctcttttaagaggtgtccagtgtcag60

ctgcagctggtggagtctgggggaggcgtggtccagcctgggaggtccctgtccctctca120

tgtgcagcctctggattcaccttcagtaactatgtcttctactgggtccgccaggttcca180

ggcaaggggctggagtgggtggcatttatatcgtttgatggcagtaataaatactatgca240

gactccgtgaaggggcgattcatcatctccagagataattccaagagcacactgtatctc300

caaatgaactccctgagagttgaggacacggctgtgtattactgtgcctggacgtctggg360

gactggggccagggaaccctggtcaccgtcgcctcagcctccaccaagggcccatcggtc420

ttccccctggcaccctcctccaagagcacctctgggggcacagcggccctgggctgcctg480

gtcaaggactacttccccgaaccggtgacggtgtcgtggaactcaggcgccctgaccagc540

ggcgtgcacaccttcccggctgtcctacagtcctcaggactctactccctcagcagcgtg600

gtgaccgtgccctccagcagcttgggcacccagacctacatctgcaacgtgaatcacaag660

cccagcaacaccaaggtggacaagagagttgagcccaaatcttgtgacaaaactcacaca720

tgcccaccgtgcccagcacctgaactcctggggggaccgtcagtcttcctcttcccccca780

aaacccaaggacaccctcatgatctcccggacccctgaggtcacatgcgtggtggtggac840

gtgagccacgaagaccctgaggtcaagttcaactggtacgtggacggcgtggaggtgcat900

aatgccaagacaaagccgcgggaggagcagtacaacagcacgtaccgtgtggtcagcgtc960

ctcaccgtcctgcaccaggactggctgaatggcaaggagtacaagtgcaaggtctccaac1020

aaagccctcccagcccccatcgagaaaaccatctccaaagccaaagggcagccccgagaa1080

ccacaggtgtacaccctgcccccatcccgggatgagctgaccaagaaccaggtcagcctg1140

acctgcctggtcaaaggcttctatcccagcgacatcgccgtggagtgggagagcaatggg1200

cagccggagaacaactacaagaccacgcctcccgtgctggactccgacggctccttcttc1260

ctctacagcaagctcaccgtggacaagagcaggtggcagcaggggaacgtcttctcatgc1320

tccgtgatgcatgaggctctgcacaaccactacacgcagaagagcctctccctgtctccg1380

ggtaaatga1389

<210>15

<211>1458

<212>dna

<213>igg1

<400>15

atggagtttgggctgagctgggttttcctcgttgctcttttaagaggtgtccagtgtcag60

gtgcagctggtggagtctgggggaggcgtggtccagcctgggaggtccctgagactctcc120

tgtgcagcgtctggattcaccttcagtagctatggcatgcactgggtccgccaggctcca180

ggcaaggggctggagtgggtggcagttatatggtatgatggaagtaataaatactatgca240

gactccgtgaagggccgattcaccatctccagagacaattccaagaacacgctgtatctg300

caaatgaacagcctgagagccgaggacacggctgtgtattactgtgcgagaggggacgtt360

cagtattacgatttttggagtggttattatgatccccgtataactggaacgactggctac420

ggtatggacgtctggggccaagggaccacggtcaccgtctcctcagcctccaccaagggc480

ccatcggtcttccccctggcaccctcctccaagagcacctctgggggcacagcggccctg540

ggctgcctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactcaggcgcc600

ctgaccagcggcgtgcacaccttcccggctgtcctacagtcctcaggactctactccctc660

agcagcgtggtgaccgtgccctccagcagcttgggcacccagacctacatctgcaacgtg720

aatcacaagcccagcaacaccaaggtggacaagagagttgagcccaaatcttgtgacaaa780

actcacacatgcccaccgtgcccagcacctgaactcctggggggaccgtcagtcttcctc840

ttccccccaaaacccaaggacaccctcatgatctcccggacccctgaggtcacatgcgtg900

gtggtggacgtgagccacgaagaccctgaggtcaagttcaactggtacgtggacggcgtg960

gaggtgcataatgccaagacaaagccgcgggaggagcagtacaacagcacgtaccgtgtg1020

gtcagcgtcctcaccgtcctgcaccaggactggctgaatggcaaggagtacaagtgcaag1080

gtctccaacaaagccctcccagcccccatcgagaaaaccatctccaaagccaaagggcag1140

ccccgagaaccacaggtgtacaccctgcccccatcccgggatgagctgaccaagaaccag1200

gtcagcctgacctgcctggtcaaaggcttctatcccagcgacatcgccgtggagtgggag1260

agcaatgggcagccggagaacaactacaagaccacgcctcccgtgctggactccgacggc1320

tccttcttcctctacagcaagctcaccgtggacaagagcaggtggcagcaggggaacgtc1380

ttctcatgctccgtgatgcatgaggctctgcacaaccactacacgcagaagagcctctcc1440

ctgtctccgggtaaatga1458

<210>16

<211>1452

<212>dna

<213>igg1

<400>16

atggagtttgggctgagctgggttttcctcgttgctcttttaagaggtgtccagtgtcag60

gtgcagctggtggagtctgggggaggcgtggtccagcctgggaggtccctgagactctcc120

tgtgcagcctctggattcaccttcagtagctatgctatgcactgggtccgccaggctcca180

ggcaaggggctggagtgggtggcagttatatcatatgatggaagcaataaatactacgca240

gactccgtgaagggccgattcaccatctccagagacaattccaagaacacgctgtatctg300

caaatgaacagcctgagagctgaggacacggctgtgtattactgtgcgagagatatacag360

tattacgatttttggagtggttaccttcaaaccaattattactactactactacggtatg420

gacgtctggggccaagggaccacggtcaccgtctcctcagcctccaccaagggcccatcg480

gtcttccccctggcaccctcctccaagagcacctctgggggcacagcggccctgggctgc540

ctggtcaaggactacttccccgaaccggtgacggtgtcgtggaactcaggcgccctgacc600

agcggcgtgcacaccttcccggctgtcctacagtcctcaggactctactccctcagcagc660

gtggtgaccgtgccctccagcagcttgggcacccagacctacatctgcaacgtgaatcac720

aagcccagcaacaccaaggtggacaagaaagttgagcccaaatcttgtgacaaaactcac780

acatgcccaccgtgcccagcacctgaactcctggggggaccgtcagtcttcctcttcccc840

ccaaaacccaaggacaccctcatgatctcccggacccctgaggtcacatgcgtggtggtg900

gacgtgagccacgaagaccctgaggtcaagttcaactggtacgtggacggcgtggaggtg960

cataatgccaagacaaagccgcgggaggagcagtacaacagcacgtaccgtgtggtcagc1020

gtcctcaccgtcctgcaccaggactggctgaatggcaaggagtacaagtgcaaggtctcc1080

aacaaagccctcccagcccccatcgagaaaaccatctccaaagccaaagggcagccccga1140

gaaccacaggtgtacaccctgcccccatcccgggatgagctgaccaagaaccaggtcagc1200

ctgacctgcctggtcaaaggcttctatcccagcgacatcgccgtggagtgggagagcaat1260

gggcagccggagaacaactacaagaccacgcctcccgtgctggactccgacggctccttc1320

ttcctctacagcaagctcaccgtggacaagagcaggtggcagcaggggaacgtcttctca1380

tgctccgtgatgcatgagggtctgcacaaccactacacgcagaagagcctctccctgtct1440

ccgggtaaatga1452

<210>17

<211>1434

<212>dna

<213>igg1

<400>17

atggagttttggctgagctgggttttccttgttgctattttaaaaggtgtccagtgtgag60

gtgcagctggtggagtctggaggaggcttggtccagcctggggggtccctgagactctcc120

tgtgcagcctctgggttcaccgtcagtagcaactacatgagctgggtccgccaggctcca180

gggaaggggctggagtgggtctcagttatttatagcggtggtagcacagactacgcagac240

tccgtgaagggccgattcaccatctccagacacaattccaagaacacgctgtatcttcaa300

atgaacagcctgagagctgaggacacggccgtgtattactgtgcgagagagaacttgtat360

tacgatttttggagtggttattacgtgtctggctggtacttcgatctctggggccgtggc420

accctggtcactgtctcctcagcctccaccaagggcccatcggtcttccccctggcaccc480

tcctccaagagcacctctgggggcacagcggccctgggctgcctggtcaaggactacttc540

cccgaaccggtgacggtgtcgtggaactcaggcgccctgaccagcggcgtgcacaccttc600

ccggctgtcctacagtcctcaggactctactccctcagcagcgtggtgaccgtgccctcc660

agcagcttgggcacccagacctacatctgcaacgtgaatcacaagcccagcaacaccaag720

gtggacaagagagttgagcccaaatcttgtgacaaaactcacacatgcccaccgtgccca780

gcacctgaactcctggggggaccgtcagtcttcctcttccccccaaaacccaaggacacc840

ctcatgatctcccggacccctgaggtcacatgcgtggtggtggacgtgagccacgaagac900

cctgaggtcaagttcaactggtacgtggacggcgtggaggtgcataatgccaagacaaag960

ccgcgggaggagcagtacaacagcacgtaccgtgtggtcagcgtcctcaccgtcctgcac1020

caggactggctgaatggcaaggagtacaagtgcaaggtctccaacaaagccctcccagcc1080

cccatcgagaaaaccatctccaaagccaaagggcagccccgaagaccacaggtgtacacc1140

ctgcccccatcccgggatgagctgaccaagaaccaggtcagcctgacctgcctggtcaaa1200

ggcttctatcccagcgacatcgccgtggagtgggagagcaatgggcagccggagaacaac1260

tacaagaccacgcctcccgtgctggactccgacggctccttcttcctctacagcaagctc1320

accgtggacaagagcaggtggcagcaggggaacgtcttctcatgctccgtgatgcatgag1380

gctctgcacaaccactacacgcagaagagcctctccctgtctccgggtaaatga1434

<210>18

<211>467

<212>prt

<213>igg1

<400>18

metaspthrleucysserthrleuleuleuleuthrileprosertrp

151015

valleuserglnilethrleulysgluserglyprothrleuvalarg

202530

prothrglnthrleuthrleuthrcysthrpheserglypheserleu

354045

serthrthrglyvalglyvalglytrpileargglnproproglylys

505560

alaleuglutrpleuthrleuiletyrtrpaspaspasplysargtyr

65707580

argproserleuglnserargleuthrilethrlysaspthrserlys

859095

asnglnvalvalleuthrmetthrasnmetaspprovalaspthrgly

100105110

thrtyrtyrcysalahisvalhisleuserglyleuglytyrtrpgly

115120125

glnglyalaleuvalthrvalserseralaserthrlysglyproser

130135140

valpheproleualaproserserlysserthrserglyglythrala

145150155160

alaleuglycysleuvallysasptyrpheprogluprovalthrval

165170175

sertrpasnserglyalaleuthrserglyvalhisthrpheproala

180185190

valleuglnserserglyleutyrserleuserservalvalthrval

195200205

proserserserleuglythrglnthrtyrilecysasnvalasnhis

210215220

lysproserasnthrlysvalasplyslysvalgluprolyssercys

225230235240

asplysthrhisthrcysproprocysproalaprogluleuleugly

245250255

glyproservalpheleupheproprolysprolysaspthrleumet

260265270

ileserargthrprogluvalthrcysvalvalvalaspvalserhis

275280285

gluaspprogluvallyspheasntrptyrvalaspglyvalgluval

290295300

hisasnalalysthrlysproargglugluglntyrasnserthrtyr

305310315320

argvalvalservalleuthrvalleuhisglnasptrpleuasngly

325330335

lysglutyrlyscyslysvalserasnlysalaleuproalaproile

340345350

glulysthrileserlysalalysglyglnproarggluproglnval

355360365

tyrthrleuproproserarggluglumetthrlysasnglnvalser

370375380

leuthrcysleuvallysglyphetyrproseraspilealavalglu

385390395400

trpgluserasnglyglnprogluasnasntyrlysthrthrpropro

405410415

metleuaspseraspglyserphepheleutyrserlysleuthrval

420425430

asplysserargtrpglnglnglyasnvalphesercysservalmet

435440445

hisglualaleuhisasnhistyrthrglnlysserleuserleuser

450455460

proglylys

465

<210>19

<211>480

<212>prt

<213>igg1

<400>19

metaspleuleuhislysasnmetlyshisleutrpphepheleuleu

151015

leuvalalaalaproargtrpvalleuserglnvalglnvalglngln

202530

trpglyalaglyleuleulysprosergluthrleuserleuthrcys

354045

alavaltyrglyglyserpheserglytyrtyrtrpsertrpilearg

505560

glnproproglylysglyleuglutrpileglygluileasnhisser

65707580

glyserthrasntyrasnproserleulysserargvalserileser

859095

valaspthrserlysasnglnpheserleulysleuserservalthr

100105110

alaalaaspthralavalphetyrcysalaargglyargtyrserser

115120125

thrtrptyrglyglyargglytrppheaspprotrpglyglnglythr

130135140

leuvalthrvalserseralaserthrlysglyproservalphepro

145150155160

leualaproserserlysserthrserglyglythralaalaleugly

165170175

cysleuvallysasptyrpheprogluprovalthrvalsertrpasn

180185190

serglyalaleuthrserglyvalhisthrpheproalavalleugln

195200205

serserglyleutyrserleuserservalvalthrvalproserser

210215220

serleuglythrglnthrtyrilecysasnvalasnhislysproser

225230235240

asnthrlysvalasplyslysvalgluprolyssercysasplysthr

245250255

histhrcysproprocysproalaprogluleuleuglyglyproser

260265270

valpheleupheproprolysprolysaspthrleumetileserarg

275280285

thrprogluvalthrcysvalvalvalaspvalserhisgluasppro

290295300

gluvallyspheasntrptyrvalaspglyvalgluvalhisasnala

305310315320

lysthrlysproargglugluglntyrasnserthrtyrargvalval

325330335

servalleuthrvalleuhisglnasptrpleuasnglylysglutyr

340345350

lyscyslysvalserasnlysalaleuproalaproileglulysthr

355360365

ileserlysalalysglyglnproarggluproglnvaltyrthrleu

370375380

proproserargaspgluleuthrlysasnglnvalserleuthrcys

385390395400

leuvallysglyphetyrproseraspilealavalglutrpgluser

405410415

asnglyglnprogluasnasntyrlysthrthrproprovalleuasp

420425430

seraspglyserphepheleutyrserlysleuthrvalasplysser

435440445

argtrpglnglnglyasnvalphesercysservalmethisgluala

450455460

leuhisasnhistyrthrglnlysserleuserleuserproglylys

465470475480

<210>20

<211>475

<212>prt

<213>igg1

<400>20

metasptrpthrtrpserileleupheleuvalalaalaalathrgly

151015

alahisserglnglypheleuvalglnserglyalagluvallyslys

202530

proglyalaserleulysvalsercyslysalaserglytyrthrphe

354045

thrhistyrglyileasntrpvalargglnalaproglyglnglyleu

505560

glutrpmetglytrpileserglytyrasnglyasnthrhistyrala

65707580

glnlyspheglnglyargleuthrmetthrthraspthrserthrser

859095

thralatyrmetgluleuargglyleuargseraspaspthralaval

100105110

tyrtyrcysalaargaspleuphevalthrargasptyrtyrglyser

115120125

glythrglytyrglyasptrpglyglnglythrleuvalthrvalser

130135140

seralaserthrlysglyproservalpheproleualaproserser

145150155160

lysserthrserglyglythralaalaleuglycysleuvallysasp

165170175

tyrpheprogluprovalthrvalsertrpasnserglyalaleuthr

180185190

serglyvalhisthrpheproalavalleuglnserserglyleutyr

195200205

serleuserservalvalthrvalproserserserleuglythrgln

210215220

thrtyrilecysasnvalasnhislysproserasnthrlysvalasp

225230235240

lyslysvalgluprolyssercysasplysthrhisthrcyspropro

245250255

cysproalaprogluleuleuglyglyproservalpheleuphepro

260265270

prolysprolysaspthrleumetileserargthrprogluvalthr

275280285

cysvalvalvalaspvalserhisgluaspprogluvallyspheasn

290295300

trptyrvalaspglyvalgluvalhisasnalalysthrlysproarg

305310315320

glugluglntyrasnserthrtyrargvalvalservalleuthrval

325330335

leuhisglnasptrpleuasnglylysglutyrlyscyslysvalser

340345350

asnlysalaleuproalaproileglulysthrileserlysalalys

355360365

glyglnproarggluproglnvaltyrthrleuproproserargasp

370375380

gluleuthrlysasnglnvalserleuthrcysleuvallysglyphe

385390395400

tyrproseraspilealavalglutrpgluserasnglyglnproglu

405410415

asnasntyrlysthrthrproprovalleuaspseraspglyserphe

420425430

pheleutyrserlysleuthrvalasplysserargtrpglnglngly

435440445

asnvalphesercysservalmethisglualaleuhisasnhistyr

450455460

thrglnlysserleuserleuserproglylys

465470475

<210>21

<211>471

<212>prt

<213>igg1

<400>21

metasptrpthrtrpargpheleuphevalvalalaalaalathrgly

151015

valglnserglnvalhisleuvalglnserglyalagluvallyslys

202530

proglyserservallysvalsercyslysalaserglyglythrphe

354045

aspthrasnalaileasntrpvalargglnvalproglyleuglyleu

505560

glutrpmetglyargileileprovalleuglythrvalasnserala

65707580

hisasnpheglnglyargvalthrilethralaasplysserthrasn

859095

thralatyrmetgluvalserserleuargsergluaspthralaval

100105110

tyrtyrcysalaargaspargglythrthrglytyrtyrtyrtyrmet

115120125

aspleutrpglylysglythrthrvalthrvalserseralaserthr

130135140

lysglyproservalpheproleualaproserserlysserthrser

145150155160

glyglythralaalaleuglycysleuvallysasptyrpheproglu

165170175

provalthrvalsertrpasnserglyalaleuthrserglyvalhis

180185190

thrpheproalavalleuglnserserglyleutyrserleuserser

195200205

valvalthrvalproserserserleuglythrglnthrtyrilecys

210215220

asnvalasnhislysproserasnthrlysvalasplysargvalglu

225230235240

prolyssercysasplysthrhisthrcysproprocysproalapro

245250255

gluleuleuglyglyproservalpheleupheproprolysprolys

260265270

aspthrleumetileserargthrprogluvalthrcysvalvalval

275280285

aspvalserhisgluaspprogluvallyspheasntrptyrvalasp

290295300

glyvalgluvalhisasnalalysthrlysproargglugluglntyr

305310315320

asnserthrtyrargvalvalservalleuthrvalleuhisglnasp

325330335

trpleuasnglylysglutyrlyscyslysvalserasnlysalaleu

340345350

proalaproileglulysthrileserlysalalysglyglnproarg

355360365

gluproglnvaltyrthrleuproproserargaspgluleuthrlys

370375380

asnglnvalserleuthrcysleuvallysglyphetyrproserasp

385390395400

ilealavalglutrpgluserasnglyglnprogluasnasntyrlys

405410415

thrthrproprovalleuaspseraspglyserphepheleutyrser

420425430

lysleuthrvalasplysserargtrpglnglnglyasnvalpheser

435440445

cysservalmethisglualaleuhisasnhistyrthrglnlysser

450455460

leuserleuserproglylys

465470

<210>22

<211>470

<212>prt

<213>igg1

<400>22

metasptrpthrtrpargpheleuphevalvalalaalaalathrgly

151015

valglnserglnvalargleuvalglnserglyalagluvallyslys

202530

proglyserservallysvalsercyslysalaserglyglythrphe

354045

serthrphealailesertrpvalargglnalaproglyglnglyleu

505560

glutrpmetglyglyileileproileleuglythralatyrtyrser

65707580

glnasnpheglnglyargvalthrilethralaaspgluserthrser

859095

thralahismetgluleuserserleuilesergluaspthralaval

100105110

tyrtyrcysalaargalatrpsersertyrtyrtyrtyralametasp

115120125

valtrpglyglnglythrthrvalthrvalserseralaserthrlys

130135140

glyproservalpheproleualaproserserlysserthrsergly

145150155160

glythralaalaleuglycysleuvallysasptyrpheproglupro

165170175

valthrvalsertrpasnserglyalaleuthrserglyvalhisthr

180185190

pheproalavalleuglnserserglyleutyrserleuserserval

195200205

valthrvalproserserserleuglythrglnthrtyrilecysasn

210215220

valasnhislysproserasnthrlysvalasplyslysvalglupro

225230235240

lyssercysasplysthrhisthrcysproprocysproalaproglu

245250255

leuleuglyglyproservalpheleupheproprolysprolysasp

260265270

thrleumetileserargthrprogluvalthrcysvalvalvalasp

275280285

valserhisgluaspprogluvallyspheasntrptyrvalaspgly

290295300

valgluvalhisasnalalysthrlysproargglugluglntyrasn

305310315320

serthrtyrargvalvalservalleuthrvalleuhisglnasptrp

325330335

leuasnglylysglutyrlyscyslysvalserasnlysalaleupro

340345350

alaproileglulysthrileserlysalalysglyglnproargglu

355360365

proglnvaltyrthrleuproproserargaspgluleuthrlysasn

370375380

glnvalserleuthrcysleuvallysglyphetyrproseraspile

385390395400

alavalglutrpgluserasnglyglnprogluasnasntyrlysthr

405410415

thrproprovalleuaspseraspglyserphepheleutyrserlys

420425430

leuthrvalasplysserargtrpglnglnglyasnvalphesercys

435440445

servalmethisglualaleuhisasnhistyrthrglnlysserleu

450455460

serleuserproglylys

465470

<210>23

<211>470

<212>prt

<213>igg1

<400>23

metglupheglyleusertrpvalpheleuvalalaleuleuarggly

151015

valglncysglnvalglnleuvalgluserglyglyglyvalvalgln

202530

proglyargserleuargleusercysalaalaserglyphethrphe

354045

sersertyralamethistrpvalargglnalaproglylysglyleu

505560

glutrpvalalavalilesertyraspglyserasnlystyrtyrala

65707580

aspservallysglyargphethrileserargaspasnserlysasn

859095

thrleutyrleuglnmetasnserleuargalagluaspthralaval

100105110

tyrtyrcysalaargglyargthrthrglytyrtyrtyrglymetasp

115120125

valtrpglyglnglythrthrvalthrvalserseralaserthrlys

130135140

glyproservalpheproleualaproserserlysserthrsergly

145150155160

glythralaalaleuglycysleuvallysasptyrpheproglupro

165170175

valthrvalsertrpasnserglyalaleuthrserglyvalhisthr

180185190

pheproalavalleuglnserserglyleutyrserleuserserval

195200205

valthrvalproserserserleuglythrglnthrtyrilecysasn

210215220

valasnhislysproserasnthrlysvalasplyslysvalglupro

225230235240

lyssercysasplysthrhisthrcysproprocysproalaproglu

245250255

leuleuglyglyproservalpheleupheproprolysprolysasp

260265270

thrleumetileserargthrprogluvalthrcysvalvalvalasp

275280285

valserhisgluaspprogluvallyspheasntrptyrvalaspgly

290295300

valgluvalhisasnalalysthrlysproargglugluglntyrasn

305310315320

serthrtyrargvalvalservalleuthrvalleuhisglnasptrp

325330335

leuasnglylysglutyrlyscyslysvalserasnlysalaleupro

340345350

alaproileglulysthrileserlysalalysglyglnproargglu

355360365

proglnvaltyrthrleuproproserargaspgluleuthrlysasn

370375380

glnvalserleuthrcysleuvallysglyphetyrproseraspile

385390395400

alavalglutrpgluserasnglyglnprogluasnasntyrlysthr

405410415

thrproprovalleuaspseraspglyserphepheleutyrserlys

420425430

leuthrvalasplysserargtrpglnglnglyasnvalphesercys

435440445

servalmethisglualaleuhisasnhistyrthrglnlysserleu

450455460

serleuserproglylys

465470

<210>24

<211>470

<212>prt

<213>igg1

<400>24

metglupheglyleusertrpvalpheleuvalalaleuleuarggly

151015

valglncysglnvalglnleuvalgluserglyglyglyvalvalgln

202530

proglyargserleuargleusercysalaalaserglyphethrphe

354045

sersertyralaleuhistrpvalargglnalaproglylysglyleu

505560

glutrpvalalavalilesertyraspglyserasnlystyrtyrala

65707580

aspservallysglyargphethrileserargaspasnserlysasn

859095

thrleutyrleuglnmetasnserleuargalagluaspthralaval

100105110

tyrtyrcysalaargserserserglysertyrtyrhistyrpheasp

115120125

tyrtrpglyglnglythrleuvalthrvalserseralaserthrlys

130135140

glyproservalpheproleualaproserserlysserthrsergly

145150155160

glythralaalaleuglycysleuvallysasptyrpheproglupro

165170175

valthrvalsertrpasnserglyalaleuthrserglyvalhisthr

180185190

pheproalavalleuglnserserglyleutyrserleuserserval

195200205

valthrvalproserserserleuglythrglnthrtyrilecysasn

210215220

valasnhislysproserasnthrlysvalasplyslysvalglupro

225230235240

lyssercysasplysthrhisthrcysproprocysproalaproglu

245250255

leuleuglyglyproservalpheleupheproprolysprolysasp

260265270

thrleumetileserargthrprogluvalthrcysvalvalvalasp

275280285

valserhisgluaspprogluvallyspheasntrptyrvalaspgly

290295300

valgluvalhisasnalalysthrlysproargglugluglntyrasn

305310315320

serthrtyrargvalvalservalleuthrvalleuhisglnasptrp

325330335

leuasnglylysglutyrlyscyslysvalserasnlysalaleupro

340345350

alaproileglulysthrileserlysalalysglyglnproargglu

355360365

proglnvaltyrthrleuproproserargaspgluleuthrlysasn

370375380

glnvalserleuthrcysleuvallysglyphetyrproseraspile

385390395400

alavalglutrpgluserasnglyglnprogluasnasntyrlysthr

405410415

thrproprovalleuaspseraspglyserphepheleutyrserlys

420425430

leuthrvalasplysserargtrpglnglnglyasnvalphesercys

435440445

servalmethisglualaleuhisasnhistyrthrglnlysserleu

450455460

serleuserproglylys

465470

<210>25

<211>470

<212>prt

<213>igg1

<400>25

metglupheglyleusertrpvalpheleuvalalaleuleuarggly

151015

valglncysglnvalglnleuvalgluserglyglyglyvalvalarg

202530

proglyargserleuargleusercysalaalaserglyphethrphe

354045

sersertyralaleuhistrpvalargglnalaproglylysglyleu

505560

glutrpvalalavalilesertyraspglyserasnlystyrtyrala

65707580

aspservallysglyargphethrileserargaspasnserlysasn

859095

thrleutyrleuglnmetasnserleuargalagluaspthralaval

100105110

tyrtyrcysalaargserserserglysertyrtyrhistyrpheasp

115120125

tyrtrpglyglnglythrleuvalthrvalserseralaserthrlys

130135140

glyproservalpheproleualaproserserlysserthrsergly

145150155160

glythralaalaleuglycysleuvallysasptyrpheproglupro

165170175

valthrvalsertrpasnserglyalaleuthrserglyvalhisthr

180185190

pheproalavalleuglnserserglyleutyrserleuserserval

195200205

valthrvalproserserserleuglythrglnthrtyrilecysasn

210215220

valasnhislysproserasnthrlysvalasplyslysvalglupro

225230235240

lyssercysasplysthrhisthrcysproprocysproalaproglu

245250255

leuleuglyglyproservalpheleupheproprolysprolysasp

260265270

thrleumetileserargthrprogluvalthrcysvalvalvalasp

275280285

valserhisgluaspprogluvallyspheasntrptyrvalaspgly

290295300

valgluvalhisasnalalysthrlysproargglugluglntyrasn

305310315320

serthrtyrargvalvalservalleuthrvalleuhisglnasptrp

325330335

leuasnglylysglutyrlyscyslysvalserasnlysalaleupro

340345350

alaproileglulysthrileserlysalalysglyglnproargglu

355360365

proglnvaltyrthrleuproproserargaspgluleuthrlysasn

370375380

glnvalserleuthrcysleuvallysglyphetyrproseraspile

385390395400

alavalglutrpgluserasnglyglnprogluasnasntyrlysthr

405410415

thrproprovalleuaspseraspglyserphepheleutyrserlys

420425430

leuthrvalasplysserargtrpglnglnglyasnvalphesercys

435440445

servalmethisglualaleuhisasnhistyrthrglnlysserleu

450455460

serleuserproglylys

465470

<210>26

<211>477

<212>prt

<213>igg1

<400>26

metasptrpthrtrpargpheleuphevalvalalaalaalathrgly

151015

valglnserglnvalglnleuvalglnserglyalagluvallysarg

202530

proglyserservallysvalsercyslysalaserglyglythrphe

354045

sersertyralailesertrpvalargglnalaproglyglnglyleu

505560

glutrpmetglyglyileileprometpheglythralaasnhisala

65707580

glnlyspheglnglyargilethrilethralaglygluserthrasn

859095

thralatyrmetgluleuserserleuargsergluaspthralaval

100105110

tyrtyrcysalaargglyvaltyrcysglyglyaspcysprothrarg

115120125

tyrtyrtyrtyrglymetaspvaltrpglyglnglythrthrvalthr

130135140

valserseralaserthrlysglyproservalpheproleualapro

145150155160

serserlysserthrserglyglythralaalaleuglycysleuval

165170175

lysasptyrpheprogluprovalthrvalsertrpasnserglyala

180185190

leuthrserglyvalhisthrpheproalavalleuglnsersergly

195200205

leutyrserleuserservalvalthrvalproserserserleugly

210215220

thrglnthrtyrilecysasnvalasnhislysproserasnthrlys

225230235240

valasplyslysvalgluprolyssercysasplysthrhisthrcys

245250255

proprocysproalaprogluleuleuglyglyproservalpheleu

260265270

pheproprolysprolysaspthrleumetileserargthrproglu

275280285

valthrcysvalvalvalaspvalserhisgluaspprogluvallys

290295300

pheasntrptyrvalaspglyvalgluvalhisasnalalysthrlys

305310315320

proargglugluglntyrasnserthrtyrargvalvalservalleu

325330335

thrvalleuhisglnasptrpleuasnglylysglutyrlyscyslys

340345350

valserasnlysalaleuproalaproileglulysthrileserlys

355360365

alalysglyglnproarggluproglnvaltyrthrleuproproser

370375380

argaspgluleuthrlysasnglnvalserleuthrcysleuvallys

385390395400

glyphetyrproseraspilealavalglutrpgluserasnglygln

405410415

progluasnasntyrlysthrthrproprometleuaspseraspgly

420425430

serphepheleutyrserlysleuthrvalasplysserargtrpgln

435440445

glnglyasnvalphesercysservalmethisglualaleuhisasn

450455460

histyrthrglnlysserleuserleuserproglylys

465470475

<210>27

<211>481

<212>prt

<213>igg1

<400>27

metgluphetrpleusertrpvalpheleuvalalaileleulysgly

151015

valglncysglumethisleuvalgluserglyglyglyleuvalgln

202530

proglyglyserleuargleusercysalavalserglyphethrval

354045

serserasnhisleuasntrpvalargglnalaproglylysglyleu

505560

glutrpvalservaliletyrserglyglyserthrtyrtyralaasp

65707580

servalgluglyargphethrileserargaspasnserlysasnthr

859095

leutyrleuglnmetasnserleuargglygluaspthralavaltyr

100105110

tyrcysalaarggluasnservalalatrppheglygluleuleuphe

115120125

asnargglygluasntyrtyrtyrglymetaspvaltrpglyglngly

130135140

thrthrvalthrvalserseralaserthrlysglyproservalphe

145150155160

proleualaproserserlysserthrserglyglythralaalaleu

165170175

glycysleuvallysasptyrpheprogluprovalthrvalsertrp

180185190

asnserglyalaleuthrserglyvalhisthrpheproalavalleu

195200205

glnserserglyleutyrserleuserservalvalthrvalproser

210215220

serserleuglythrglnthrtyrilecysasnvalasnhislyspro

225230235240

serasnthrlysvalasplysargvalgluprolyssercysasplys

245250255

thrhisthrcysproprocysproalaprogluleuleuglyglypro

260265270

servalpheleupheproprolysprolysaspthrleumetileser

275280285

argthrprogluvalthrcysvalvalvalaspvalserhisgluasp

290295300

progluvallyspheasntrptyrvalaspglyvalgluvalhisasn

305310315320

alalysthrlysproargglugluglntyrasnserthrtyrargval

325330335

valservalleuthrvalleuhisglnasptrpleuasnglylysglu

340345350

tyrlyscyslysvalserasnlysalaleuproalaproileglulys

355360365

thrileserlysalalysglyglnproarggluproglnvaltyrthr

370375380

leuproproserargaspgluleuthrlysasnglnvalserleuthr

385390395400

cysleuvallysglyphetyrproseraspilealavalglutrpglu

405410415

serasnglyglnprogluasnasntyrlysthrthrproprovalleu

420425430

aspseraspglyserphepheleutyrserlysleuthrvalasplys

435440445

serargtrpglnglnglyasnvalphesercysservalmethisglu

450455460

alaleuhisasnhistyrthrglnlysserleuserleuserprogly

465470475480

lys

<210>28

<211>466

<212>prt

<213>igg1

<400>28

metgluleuglyleusertrpvalpheleuvalalaileleuglugly

151015

valglncysgluvalglnleuvalgluserglyglyglyleuvalgln

202530

proglyglyserleuargleusercysalaalaserglyphethrphe

354045

sersertyraspmethistrpvalargglnalathrglylysglyleu

505560

glutrpvalseralaileglythralaglyaspthrtyrtyrprogly

65707580

servallysglyargphethrileserarggluasnalalysasnser

859095

leutyrleuglnmetasnserleuargalaglyaspthralavaltyr

100105110

tyrcysalaargaspleusertyrtyrglymetaspvaltrpglygln

115120125

glythrthrvalthrvalserseralaserthrlysglyproserval

130135140

pheproleualaproserserlysserthrserglyglythralaala

145150155160

leuglycysleuvallysasptyrpheprogluprovalthrvalser

165170175

trpasnserglyalaleuthrserglyvalhisthrpheproalaval

180185190

leuglnserserglyleutyrserleuserservalvalthrvalpro

195200205

serserserleuglythrglnthrtyrilecysasnvalasnhislys

210215220

proserasnthrlysvalasplyslysvalgluprolyssercysasp

225230235240

lysthrhisthrcysproprocysproalaprogluleuleuglygly

245250255

proservalpheleupheproprolysprolysaspthrleumetile

260265270

serargthrprogluvalthrcysvalvalvalaspvalserhisglu

275280285

aspprogluvallyspheasntrptyrvalaspglyvalgluvalhis

290295300

asnalalysthrlysproargglugluglntyrasnserthrtyrarg

305310315320

valvalservalleuthrvalleuhisglnasptrpleuasnglylys

325330335

glutyrlyscyslysvalserasnlysalaleuproalaproileglu

340345350

lysthrileserlysalalysglyglnproarggluproglnvaltyr

355360365

thrleuproproserargaspgluleuthrlysasnglnvalserleu

370375380

thrcysleuvallysglyphetyrproseraspilealavalglutrp

385390395400

gluserasnglyglnprogluasnasntyrlysthrthrproproval

405410415

leuaspseraspglyserphepheleutyrserlysleuthrvalasp

420425430

lysserargtrpglnglnglyasnvalphesercysservalmethis

435440445

gluglyleuhisasnhistyrthrglnlysserleuserleuserpro

450455460

glylys

465

<210>29

<211>466

<212>prt

<213>igg1

<400>29

metgluphetrpleusertrpvalpheleuvalalaileleulysgly

151015

valglncysgluvalglnleuvalgluserglyglyglyleuvalgln

202530

proglyglyserleuargleusercysalaalaserglyphethrval

354045

serserasntyrmetsertrpvalargglnalaproglylysglyleu

505560

glutrpvalservaliletyrserglyglyserthrtyrtyralaasp

65707580

servallysglyargphethrileserarghisasnserlysasnthr

859095

leutyrleuglnmetasnserleuargalagluaspthralavaltyr

100105110

tyrcysalaargaspleuserasptyrglymetaspvaltrpglygln

115120125

glythrthrvalthrvalserseralaserthrlysglyproserval

130135140

pheproleualaproserserlysserthrserglyglythralaala

145150155160

leuglycysleuvallysasptyrpheprogluprovalthrvalser

165170175

trpasnserglyalaleuthrserglyvalhisthrpheproalaval

180185190

leuglnserserglyleutyrserleuserservalvalthrvalpro

195200205

serserserleuglythrglnthrtyrilecysasnvalasnhislys

210215220

proserasnthrlysvalasplyslysvalgluprolyssercysasp

225230235240

lysthrhisthrcysproprocysproalaprogluleuleuglygly

245250255

proservalpheleupheproprolysprolysaspthrleumetile

260265270

serargthrprogluvalthrcysvalvalvalaspvalserhisglu

275280285

aspprogluvallyspheasntrptyrvalaspglyvalgluvalhis

290295300

asnalalysthrlysproargglugluglntyrasnserthrtyrarg

305310315320

valvalservalleuthrvalleuhisglnasptrpleuasnglylys

325330335

glutyrlyscyslysvalserasnlysalaleuproalaproileglu

340345350

lysthrileserlysalalysglyglnproarggluproglnvaltyr

355360365

thrleuproproserargaspgluleuthrlysasnglnvalserleu

370375380

thrcysleuvallysglyphetyrproseraspilealavalglutrp

385390395400

gluserasnglyglnprogluasnasntyrlysthrthrproproval

405410415

leuaspseraspglyserphepheleutyrserlysleuthrvalasp

420425430

lysserargtrpglnglnglyasnvalphesercysservalmethis

435440445

gluglyleuhisasnhistyrthrglnlysserleuserleuserpro

450455460

glylys

465

<210>30

<211>466

<212>prt

<213>igg1

<400>30

metgluphetrpleusertrpvalpheleuvalalaileleulysgly

151015

valglncysgluvalglnleuvalgluserglyglyglyleuvalgln

202530

proglyglyserleuargleusercysalaalaserglyphethrval

354045

serserasntyrmetsertrpvalargglnalaproglylysglyleu

505560

glutrpvalservaliletyrserglyglyserthrtyrtyralaasp

65707580

servallysglyargphethrileserarghisasnserlysasnthr

859095

leutyrleuglnmetasnserleuargalagluaspthralavaltyr

100105110

tyrcysalaargaspleuilevaltyrglymetaspvaltrpglygln

115120125

glythrthrvalthrvalserseralaserthrlysglyproserval

130135140

pheproleualaproserserlysserthrserglyglythralaala

145150155160

leuglycysleuvallysasptyrpheprogluprovalthrvalser

165170175

trpasnserglyalaleuthrserglyvalhisthrpheproalaval

180185190

leuglnserserglyleutyrserleuserservalvalthrvalpro

195200205

serserserleuglythrglnthrtyrilecysasnvalasnhislys

210215220

proserasnthrlysvalasplyslysvalgluprolyssercysasp

225230235240

lysthrhisthrcysproprocysproalaprogluleuleuglygly

245250255

proservalpheleupheproprolysprolysaspthrleumetile

260265270

serargthrprogluvalthrcysvalvalvalaspvalserhisglu

275280285

aspprogluvallyspheasntrptyrvalaspglyvalgluvalhis

290295300

asnalalysthrlysproargglugluglntyrasnserthrtyrarg

305310315320

valvalservalleuthrvalleuhisglnasptrpleuasnglylys

325330335

glutyrlyscyslysvalserasnlysalaleuproalaproileglu

340345350

lysthrileserlysalalysglyglnproarggluproglnvaltyr

355360365

thrleuproproserargaspgluleuthrlysasnglnvalserleu

370375380

thrcysleuvallysglyphetyrproseraspilealavalglutrp

385390395400

gluserasnglyglnprogluasnasntyrlysthrthrproproval

405410415

leuaspseraspglyserphepheleutyrserlysleuthrvalasp

420425430

lysserargtrpglnglnglyasnvalphesercysservalmethis

435440445

gluglyleuhisasnhistyrthrglnlysserleuserleuserpro

450455460

glylys

465

<210>31

<211>462

<212>prt

<213>igg1

<400>31

metglupheglyleusertrpvalpheleuvalalaleuleuarggly

151015

valglncysglnleuglnleuvalgluserglyglyglyvalvalgln

202530

proglyargserleuserleusercysalaalaserglyphethrphe

354045

serasntyrvalphetyrtrpvalargglnvalproglylysglyleu

505560

glutrpvalalapheileserpheaspglyserasnlystyrtyrala

65707580

aspservallysglyargpheileileserargaspasnserlysser

859095

thrleutyrleuglnmetasnserleuargvalgluaspthralaval

100105110

tyrtyrcysalatrpthrserglyasptrpglyglnglythrleuval

115120125

thrvalalaseralaserthrlysglyproservalpheproleuala

130135140

proserserlysserthrserglyglythralaalaleuglycysleu

145150155160

vallysasptyrpheprogluprovalthrvalsertrpasnsergly

165170175

alaleuthrserglyvalhisthrpheproalavalleuglnserser

180185190

glyleutyrserleuserservalvalthrvalproserserserleu

195200205

glythrglnthrtyrilecysasnvalasnhislysproserasnthr

210215220

lysvalasplysargvalgluprolyssercysasplysthrhisthr

225230235240

cysproprocysproalaprogluleuleuglyglyproservalphe

245250255

leupheproprolysprolysaspthrleumetileserargthrpro

260265270

gluvalthrcysvalvalvalaspvalserhisgluaspprogluval

275280285

lyspheasntrptyrvalaspglyvalgluvalhisasnalalysthr

290295300

lysproargglugluglntyrasnserthrtyrargvalvalserval

305310315320

leuthrvalleuhisglnasptrpleuasnglylysglutyrlyscys

325330335

lysvalserasnlysalaleuproalaproileglulysthrileser

340345350

lysalalysglyglnproarggluproglnvaltyrthrleupropro

355360365

serargaspgluleuthrlysasnglnvalserleuthrcysleuval

370375380

lysglyphetyrproseraspilealavalglutrpgluserasngly

385390395400

glnprogluasnasntyrlysthrthrproprovalleuaspserasp

405410415

glyserphepheleutyrserlysleuthrvalasplysserargtrp

420425430

glnglnglyasnvalphesercysservalmethisglualaleuhis

435440445

asnhistyrthrglnlysserleuserleuserproglylys

450455460

<210>32

<211>485

<212>prt

<213>igg1

<400>32

metglupheglyleusertrpvalpheleuvalalaleuleuarggly

151015

valglncysglnvalglnleuvalgluserglyglyglyvalvalgln

202530

proglyargserleuargleusercysalaalaserglyphethrphe

354045

sersertyrglymethistrpvalargglnalaproglylysglyleu

505560

glutrpvalalavaliletrptyraspglyserasnlystyrtyrala

65707580

aspservallysglyargphethrileserargaspasnserlysasn

859095

thrleutyrleuglnmetasnserleuargalagluaspthralaval

100105110

tyrtyrcysalaargglyaspvalglntyrtyraspphetrpsergly

115120125

tyrtyraspproargilethrglythrthrglytyrglymetaspval

130135140

trpglyglnglythrthrvalthrvalserseralaserthrlysgly

145150155160

proservalpheproleualaproserserlysserthrserglygly

165170175

thralaalaleuglycysleuvallysasptyrpheprogluproval

180185190

thrvalsertrpasnserglyalaleuthrserglyvalhisthrphe

195200205

proalavalleuglnserserglyleutyrserleuserservalval

210215220

thrvalproserserserleuglythrglnthrtyrilecysasnval

225230235240

asnhislysproserasnthrlysvalasplysargvalgluprolys

245250255

sercysasplysthrhisthrcysproprocysproalaprogluleu

260265270

leuglyglyproservalpheleupheproprolysprolysaspthr

275280285

leumetileserargthrprogluvalthrcysvalvalvalaspval

290295300

serhisgluaspprogluvallyspheasntrptyrvalaspglyval

305310315320

gluvalhisasnalalysthrlysproargglugluglntyrasnser

325330335

thrtyrargvalvalservalleuthrvalleuhisglnasptrpleu

340345350

asnglylysglutyrlyscyslysvalserasnlysalaleuproala

355360365

proileglulysthrileserlysalalysglyglnproargglupro

370375380

glnvaltyrthrleuproproserargaspgluleuthrlysasngln

385390395400

valserleuthrcysleuvallysglyphetyrproseraspileala

405410415

valglutrpgluserasnglyglnprogluasnasntyrlysthrthr

420425430

proprovalleuaspseraspglyserphepheleutyrserlysleu

435440445

thrvalasplysserargtrpglnglnglyasnvalphesercysser

450455460

valmethisglualaleuhisasnhistyrthrglnlysserleuser

465470475480

leuserproglylys

485

<210>33

<211>483

<212>prt

<213>igg1

<400>33

metglupheglyleusertrpvalpheleuvalalaleuleuarggly

151015

valglncysglnvalglnleuvalgluserglyglyglyvalvalgln

202530

proglyargserleuargleusercysalaalaserglyphethrphe

354045

sersertyralamethistrpvalargglnalaproglylysglyleu

505560

glutrpvalalavalilesertyraspglyserasnlystyrtyrala

65707580

aspservallysglyargphethrileserargaspasnserlysasn

859095

thrleutyrleuglnmetasnserleuargalagluaspthralaval

100105110

tyrtyrcysalaargaspileglntyrtyraspphetrpserglytyr

115120125

leuglnthrasntyrtyrtyrtyrtyrtyrglymetaspvaltrpgly

130135140

glnglythrthrvalthrvalserseralaserthrlysglyproser

145150155160

valpheproleualaproserserlysserthrserglyglythrala

165170175

alaleuglycysleuvallysasptyrpheprogluprovalthrval

180185190

sertrpasnserglyalaleuthrserglyvalhisthrpheproala

195200205

valleuglnserserglyleutyrserleuserservalvalthrval

210215220

proserserserleuglythrglnthrtyrilecysasnvalasnhis

225230235240

lysproserasnthrlysvalasplyslysvalgluprolyssercys

245250255

asplysthrhisthrcysproprocysproalaprogluleuleugly

260265270

glyproservalpheleupheproprolysprolysaspthrleumet

275280285

ileserargthrprogluvalthrcysvalvalvalaspvalserhis

290295300

gluaspprogluvallyspheasntrptyrvalaspglyvalgluval

305310315320

hisasnalalysthrlysproargglugluglntyrasnserthrtyr

325330335

argvalvalservalleuthrvalleuhisglnasptrpleuasngly

340345350

lysglutyrlyscyslysvalserasnlysalaleuproalaproile

355360365

glulysthrileserlysalalysglyglnproarggluproglnval

370375380

tyrthrleuproproserargaspgluleuthrlysasnglnvalser

385390395400

leuthrcysleuvallysglyphetyrproseraspilealavalglu

405410415

trpgluserasnglyglnprogluasnasntyrlysthrthrpropro

420425430

valleuaspseraspglyserphepheleutyrserlysleuthrval

435440445

asplysserargtrpglnglnglyasnvalphesercysservalmet

450455460

hisgluglyleuhisasnhistyrthrglnlysserleuserleuser

465470475480

proglylys

<210>34

<211>477

<212>prt

<213>igg1

<400>34

metgluphetrpleusertrpvalpheleuvalalaileleulysgly

151015

valglncysgluvalglnleuvalgluserglyglyglyleuvalgln

202530

proglyglyserleuargleusercysalaalaserglyphethrval

354045

serserasntyrmetsertrpvalargglnalaproglylysglyleu

505560

glutrpvalservaliletyrserglyglyserthrasptyralaasp

65707580

servallysglyargphethrileserarghisasnserlysasnthr

859095

leutyrleuglnmetasnserleuargalagluaspthralavaltyr

100105110

tyrcysalaarggluasnleutyrtyraspphetrpserglytyrtyr

115120125

valserglytrptyrpheaspleutrpglyargglythrleuvalthr

130135140

valserseralaserthrlysglyproservalpheproleualapro

145150155160

serserlysserthrserglyglythralaalaleuglycysleuval

165170175

lysasptyrpheprogluprovalthrvalsertrpasnserglyala

180185190

leuthrserglyvalhisthrpheproalavalleuglnsersergly

195200205

leutyrserleuserservalvalthrvalproserserserleugly

210215220

thrglnthrtyrilecysasnvalasnhislysproserasnthrlys

225230235240

valasplysargvalgluprolyssercysasplysthrhisthrcys

245250255

proprocysproalaprogluleuleuglyglyproservalpheleu

260265270

pheproprolysprolysaspthrleumetileserargthrproglu

275280285

valthrcysvalvalvalaspvalserhisgluaspprogluvallys

290295300

pheasntrptyrvalaspglyvalgluvalhisasnalalysthrlys

305310315320

proargglugluglntyrasnserthrtyrargvalvalservalleu

325330335

thrvalleuhisglnasptrpleuasnglylysglutyrlyscyslys

340345350

valserasnlysalaleuproalaproileglulysthrileserlys

355360365

alalysglyglnproargargproglnvaltyrthrleuproproser

370375380

argaspgluleuthrlysasnglnvalserleuthrcysleuvallys

385390395400

glyphetyrproseraspilealavalglutrpgluserasnglygln

405410415

progluasnasntyrlysthrthrproprovalleuaspseraspgly

420425430

serphepheleutyrserlysleuthrvalasplysserargtrpgln

435440445

glnglyasnvalphesercysservalmethisglualaleuhisasn

450455460

histyrthrglnlysserleuserleuserproglylys

465470475

<210>35

<211>19

<212>dna

<213>人工序列(artificialsequence)

<400>35

gtgcctgggaagtatgtac19

<210>36

<211>22

<212>dna

<213>人工序列(artificialsequence)

<400>36

tgggtgctttatttccatgctg22

<210>37

<211>18

<212>dna

<213>人工序列(artificialsequence)

<400>37

tacgtgccaagcatcctc18

<210>38

<211>21

<212>dna

<213>人工序列(artificialsequence)

<400>38

tgctgaacaggctacaagagg21

<210>39

<211>20

<212>dna

<213>人工序列(artificialsequence)

<400>39

gagcacgaggaacatgactg20

<210>40

<211>22

<212>dna

<213>人工序列(artificialsequence)

<400>40

acattcctcctctaaagaagcc22

<210>41

<211>18

<212>dna

<213>人工序列(artificialsequence)

<400>41

cggcggtgtttccattcg18

<210>42

<211>18

<212>dna

<213>人工序列(artificialsequence)

<400>42

catggagtttgggctgag18

<210>43

<211>20

<212>dna

<213>人工序列(artificialsequence)

<400>43

gtgctttctgagagtcatgg20

<210>44

<211>22

<212>dna

<213>人工序列(artificialsequence)

<400>44

tgagagtcatggacctcctgca22

<210>45

<211>21

<212>dna

<213>人工序列(artificialsequence)

<400>45

cagaggactcaccatggagtt21

<210>46

<211>19

<212>dna

<213>人工序列(artificialsequence)

<400>46

agcaccatggactggacct19

<210>47

<211>20

<212>dna

<213>人工序列(artificialsequence)

<400>47

ggtgatcagcactgaacaca20

<210>48

<211>23

<212>dna

<213>人工序列(artificialsequence)

<400>48

tgtgccccaccatggacacactt23

<210>49

<211>20

<212>dna

<213>人工序列(artificialsequence)

<400>49

gtattcagtgatcaggactg20

<210>50

<211>20

<212>dna

<213>人工序列(artificialsequence)

<400>50

tgactcctgtgccccaccat20

<210>51

<211>21

<212>dna

<213>人工序列(artificialsequence)

<400>51

ggtgtttccattcggtgatca21

<210>52

<211>22

<212>dna

<213>人工序列(artificialsequence)

<400>52

taaagaagcccctgggagcaca22

<210>53

<211>20

<212>dna

<213>人工序列(artificialsequence)

<400>53

ccgatctgatggatctaatg20

<210>54

<211>20

<212>dna

<213>人工序列(artificialsequence)

<400>54

gatctgggtgaggtttcgta20

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