rapid amplification of cDNA endscDNA末端快速扩增
1.MATERIAL AND METHODS: Based on the rapid amplification of cDNA ends(RACE)method,gradient and nested PCR protocols were used to obtain full_length cDNA in order to get .本研究应用cDNA末端快速扩增法(rapidamplificationofcDNAends,RACE)扩增此未知基因的全长,以进行下一步的基因功能研究。
3)rapid amplification of cDNA endcDNA末端快速扩增
1.Methods: According to the partial sequences deposited in GenBank,the full-length cDNA was obtained using the techniques named rapid amplification of cDNA ends,and then,the sequence was analyzed bioinformatically.方法:根据GenBank中已发表的NRDR的cDNA部分序列信息,运用cDNA末端快速扩增技术得到全长cDNA,然后利用生物信息学的方法对其进行分析。
4)rapid amplification of cDNA ends (RACE)cDNA末端快速扩增
5)RACE[英][re?s][美][res]快速cDNA末端扩增
1.The rapid amplification of cDNA ends(RACE)technique and the electronic cDNA library select mathod was applied to amplify full length of the expressing sequence tag(EST) ,which is a specfic-stage in Schistosoma japonicum adult worm, and a cDNA fragment with an ORF was identified(GenBank accession number is AY847290).利用快速cDNA末端扩增(RACE)技术结合“电子cDNA文库”筛选法,对日本血吸虫成虫期特异表达基因EST片段的全长序列进行了扩增,获得了一含完整开放性阅读框(ORF)的基因序列(GenBank中的登录号为AY847290)。
6)RACE[英][re?s][美][res]快速扩增cDNA末端
延伸阅读
分子扩增分子式：CAS号：性质：又称无性繁殖，克隆繁殖，分子扩增。通常是指用人工方法把所需要的某一供体生物的遗传物质，即DNA大分子提取出来，在离体条件下进行切割后，取其片段，或直接用人工合成的基因(DNA)在体外利用某些工具酶(限制性内切酶等)与作为载体的DNA分子(如质粒等)连接起来，然后再导入某一受体细胞中，让该外来遗传物质在其中进行正常复制和表达的过程。自20世纪70年代始，它是基因工程(gene engineering)、或微生物育种等领域中经常采用的方法。也是重要的克隆技术之一。
