T-载体,T-vector
1)T-vectorT-载体
1.Effect of Different Bivalent Ions on T-vector Making;不同二价阳离子对T-载体生成的影响
2.A method for construction of a cloning vector(T-vector) for direct ligation with PCR products was described in this passage.1/V5-His为骨架载体,利用限制性内切酶EcoR V得到平端化的线性载体,在dTTP和Taq聚合酶作用下,产生3’末端突出一个T碱基的T-载体
2)T-vectorT载体
1.Construction and identification of brain derived neurotrophic factor gene cloned into a T-vector in rat;大鼠脑源性神经营养因子基因T载体的构建及鉴定
2.Construction of a highly efficient T-vector and the cloning of the HPS domain of human tankyrase gene;一种高效T载体的构建及其对人端锚聚合酶HPS结构域的克隆
3.Construction of T-vector by XcmⅠ;利用XcmⅠ构建T载体
英文短句/例句

1.Hepatitis C Virus IRES Gene Cloning by T Vector and Sequencing丙型肝炎病毒IRES基因T载体克隆及序列分析
2.Construction and identification of antisense HBV S gene cloning by T vector反义乙型肝炎病毒S基因T载体克隆的构建
3.Construction of a universal expression T-vector and its applications通用表达型T载体的构建与真核基因的快速克隆表达
4.Construction of T-vectors and the methods of frequent questions of processingT载体构建及其构建过程中常见问题的处理方法
5.Characterization of a highly efficient expression T-vector高效表达型T克隆载体的构建及其特性
6.Experimental study on ultimate bearing capacity of damaged concrete T-beam bridge systems体系损伤混凝土T梁桥极限承载力试验
7.Construction and Transformation of Expression Vector p215t-spp of Spirulina platensis钝顶螺旋藻表达载体p215 t-spp的构建及转化
8.Construction of Mammary Gland-Specific Vector for Human Finger-Domain Lacking t-PA and Its Expression in Cells;人t-PA指形区缺失体乳腺特异性表达载体的构建及细胞表达研究
9.t-PA Lentivirus Vectors Construction and Lentivirus Production编码t-PA基因慢病毒载体的构建及其制备体系的建立
10.Recombinant Adeno-associated Virus Vector Inhibits the Expression of IL-5 in T Lymphocytes of Asthmatic Rats;重组腺相关病毒载体抑制哮喘大鼠T淋巴细胞表达白介素5
11.Expression of HCMVpp150 and Characterization of T Cell Response to HCMV Following Stimulation with Recombinant Lentivirus Encoding the Chimeric Protein IE1-pp65;HCMV pp150表达及E1-pp65慢病毒载体刺激T细胞免疫应答的研究
12.The Theoretical and Test Studies on the Load-carrying Capacity in Integral Stability of Axial Compressed Cut T-shape Columns;剖分T型钢轴心受压构件整体稳定承载力的试验与理论研究
13.Construction of Eukaryotic Expessing Vector of Tissue Plasminogen Activator (t-PA);组织型纤溶酶原激活剂(t-PA)真核表达载体的构建
14.Construction of Mammary Gland Dual-Expression Vector Containing Prolactin and t-PA Gene and Its Expression in Cells;催乳素和t-PA乳腺共表达载体构建和细胞表达研究
15.Obtaining Marker Free Transgenic Chrysanthemum Through Double T-DNA System利用双T-DNA载体系统获得无选择标记转基因菊花
16.Construction of Twin T-DNA Vector Encoding Hairpin RNA Targeting the Replicase Gene of Barley Yellow Dwarf Virus具双T-DNA区的大麦黄矮病毒复制酶基因双链RNAi植物表达载体构建
17.Plant Binary Expression Vector Construction and Genetic Transformation of the New Type HMW-GS Gene 1Dy12.1~(*t)新型HMW-GS基因1Dy12.1~(*t)植物双元表达载体的构建及其遗传转化
18.The Fusion of Synthetized T/B Cell Antigen Epitope Genes of FMDV Type O/A and the Construction of Plant Expression VectorsA、O型FMDV T/B细胞抗原表位融合基因合成及植物表达载体构建
相关短句/例句

T-vectorT载体
1.Construction and identification of brain derived neurotrophic factor gene cloned into a T-vector in rat;大鼠脑源性神经营养因子基因T载体的构建及鉴定
2.Construction of a highly efficient T-vector and the cloning of the HPS domain of human tankyrase gene;一种高效T载体的构建及其对人端锚聚合酶HPS结构域的克隆
3.Construction of T-vector by XcmⅠ;利用XcmⅠ构建T载体
3)T vectorT载体
1.The translational initiation region of hPARP-1 gene cDNA cloning by T vector;hPARP-1基因cDNA翻译起始区域的T载体克隆
2.Preparing T vector with restriction enzyme digestion;用限制性内切酶制备T载体
3.Construction of a T vector and cloning of a differentially displayed fragment of wheat;T载体的构建及对小麦差异显示片段的克隆
4)vector pMD19-TpMD19-T载体
1.Then the genes were ligated into Multiple Cloning Site(MCS) of vector pMD19-T in certain order and transformed int.方法:设计引物,以质粒pEGFP-N1为模板,进行PCR得到抗卡那霉素的DNA片断,再以变异链球菌的DNA为模板,PCR得到luxS基因上下游序列,最后将这3段DNA片断分别按顺序插入到pMD19-T载体的多克隆酶切位点中,转化大肠杆菌的感受态中,通过在卡那霉素和氨苄青霉素的培养基进行筛选。
5)PMD18-T vectorPMD18-T载体
1.The expected size of the fragment was obtained, and it was then cloned into the PMD18-T vector.以华南农业大学兽医学院传染病教研室分离的伪狂犬病毒粤A毒株 (PRVYA)的基因组为模板 ,利用聚合酶链反应 (PCR)扩增TK基因 ,获得预定大小的片段 ,将这一片段克隆到PMD18-T载体中 。
6)pUCm-T vectorpUCm-T载体
1.Two methods for gene xanA to ligate with pUCm-T vector were discussed in this thesis.本文研究了两种不同的连接方法在野油菜黄单胞菌(Xanthomonascampestris)xanA基因与pUCm-T载体连接中的连接效果,并进行了转化实验进行鉴定。
延伸阅读

SV40载体分子式:CAS号:性质:也可简称为。由于SV40病毒基因组具备:(1)由单一的共价闭合的环状DNA所组成;(2)含有多种功能的基因组的区域已正确定位;(3)病毒基因组能够自我复制。而可用作载体。野生型病毒DNA加入外源DNA后会导致DNA分子过大需缺失部分非必需DNA后亦用作载体。现在某些质粒型表达载体带有来自SV40的个别调控区,但不含病毒组的大部分编码区。通常采用的调控区是SV40DNA中的一个300bp的区段,位于病毒早期与晚期转录单位之间,含有一系列各不相同的顺式调控要素。这些调控要素包括:(1)DNA复制起始点;(2)早期与晚期mRNA转录的起始点和启动子;(3)能够激活复制起始点并可自动调节早期转录的T抗原结合位点;(4)可被细胞转录因子识别的一般由3个G/C丰富的21bp同向重复区组成的序列;(5)组成SV40早期增强子的两段72bp同向重复序列。除此之外,某些以SV40为基础的质粒载体因带有完整的SV40转录单位,激活源细胞SV40复制起始点,也可以编码SV40大T抗原。