质粒敲除,plasmid-knockout英语短句,例句大全

质粒敲除,plasmid-knockout
1)plasmid-knockout质粒敲除
2)Knockout[英]['n?ka?t][美]['nɑ'kaut]敲除
1.Knockout of the Crystalline Inclusion Protein Gene cipA and cipB in Photorhabdus luminescens by RED Homologous RecombinationRED同源重组法敲除发光光状杆菌晶体蛋白基因cipA和cipB
2.lincolnensis JLUa2,was generated by gene knockout of lmbU with homologous recombination.利用DNA同源重组原理敲除S。
3.coli W3110 chromosome using the plasmid as follow: Firstly, we knockout the lacI gene using Red-mediated recombineering with overlapping single stranded DNA oligonucleotides.对pBR32 2 Red最优重组条件进行探索后应用该质粒提供的体内同源重组功能 ,在菌株W3110体内 ,对染色体上的lac操纵子进行了基因修饰 ,包括 :①运用kan- sacB选择反选择方法和重叠引物方法敲除了阻遏基因lacI,②运用kan -sacB选择反选择方法和线性双链DNA介导的DNA重组方法将报告基因lacZ敲入lacA和lacY的位置 ,并且首次测定了报告基因lacZ在这三个结构基因位置的组成性表达情况。

英文短句/例句

1.Constract of Myostatin Knockout Porcine Fetal Fibroblast and Preliminary Study on Myostatin Knockout of Porcine;MSTN基因敲除猪胚胎成纤维细胞的构建及其基因敲除猪的初步研究
2.Renal Phenotype of Aging UT-B Knockout Mice and Adult UT-A2/UT-B Knockout Mice老年UT-B基因敲除和成年UT-A2/UT-B基因敲除鼠肾脏表型研究
3.Pregnant Phenotype Analysis of AQP3 Null Mice and AQP8 Null MiceAQP3基因敲除鼠及AQP8基因敲除鼠妊娠相关表型的研究
4.Application of Cre-LoxP System in The Gene Knockout of Bacillus anthracis and The Knockout of eag GeneCre-LoxP系统在炭疽芽胞杆菌基因敲除中的应用及eag基因的敲除
5.MMP-9 Expression Profile in Inflammatory Cells of Mip-1α Knockout Mice and Mip-1α Receptor Knockout MiceMMP-9在Mip-1α敲除小鼠和Mip-1α受体敲除小鼠炎性细胞的表达
6.KCNJ10 Potassium Channel Knockout Abolishes Endocochlear Potential;敲除KCNJ10钾离子通道使耳蜗电位消失
7.Human Carcinoma of Esophageal Eca9706 Cell DNA Polymerseβ Gene Targeting;食管癌细胞Eca9706 DNA聚合酶β基因敲除
8.Functional Characterization of Fusarium Graminearum PTC1 Gene;禾谷镰刀菌PTC1基因敲除及功能初探
9.The Impacts of Kir6.2 Knockout on MPTP-induced Acute Neurotoxicity;Kir6.2基因敲除对MPTP急性神经毒性的影响
10.Mechanisms of Cleft Palate Formation in Wnt5a Gene Knockout Mouse;Wnt5a基因敲除小鼠腭裂发生的机制研究
11.Functional Characterization of Fusarium Graminearum TEP1 Gene禾谷镰刀菌TEP1基因敲除及功能研究
12.Knock Out of Waw in Drosophila and Its Protein Purification果蝇中waw基因的敲除及其蛋白的纯化
13.Effect of Pten Gene Knock-out on Expression of Rad51 Gene and Mechanism of The EffectPten敲除对Rad51基因表达的影响及其机制
14.Study on Development and Mechanism of Arterial Calcification in Adiponectin-deficient Mice脂联素基因敲除小鼠血管钙化的研究
15.Construction of Knockout Vector for sIgM λ Light Chain Gene from DT40 CellsDT40细胞sIgMλ轻链基因敲除载体的构建
16.The effect of lipoprotein lipase gene on lipid metabolism脂蛋白脂酶基因敲除鼠的脂代谢研究
17.Deletion of Bmi-1 gene leads to skin aging in miceBmi-1基因敲除导致小鼠皮肤老化
18.Porcine α-1, 3-galactosyltransferase Gene Knock-out and HLA-G1 Gene Knock-in in Somatic Cells;猪体细胞α-1，3-半乳糖基转移酶基因的敲除并敲入HLA-G1基因的研究

相关短句/例句

Knockout[英]['n?ka?t][美]['nɑ'kaut]敲除
1.Knockout of the Crystalline Inclusion Protein Gene cipA and cipB in Photorhabdus luminescens by RED Homologous RecombinationRED同源重组法敲除发光光状杆菌晶体蛋白基因cipA和cipB
2.lincolnensis JLUa2,was generated by gene knockout of lmbU with homologous recombination.利用DNA同源重组原理敲除S。
3.coli W3110 chromosome using the plasmid as follow: Firstly, we knockout the lacI gene using Red-mediated recombineering with overlapping single stranded DNA oligonucleotides.对pBR32 2 Red最优重组条件进行探索后应用该质粒提供的体内同源重组功能 ,在菌株W3110体内 ,对染色体上的lac操纵子进行了基因修饰 ,包括 :①运用kan- sacB选择反选择方法和重叠引物方法敲除了阻遏基因lacI,②运用kan -sacB选择反选择方法和线性双链DNA介导的DNA重组方法将报告基因lacZ敲入lacA和lacY的位置 ,并且首次测定了报告基因lacZ在这三个结构基因位置的组成性表达情况。
3)gene deletion敲除
1.Construction and application of a vector for gene deletion in gram-negative bacteria革兰阴性菌基因敲除载体的构建及其应用
2.After the construction,the vector was used to carry out gene deletion experiment in the yeeZ gene from Citrobacter freundii.目的构建1个适用于革兰阴性菌的精确基因敲除载体并证明其有效性。
4)plasmid curing质粒消除
1.Transformation of Escherichia coli and Bacillus thuringiensis and their plasmid curing by electroporation;电穿孔法用于大肠杆菌和苏云金杆菌的转化及质粒消除
2.Plasmid curing experiment was carried out by using the sacB positive selection method and plasmid cured derivatives,2020D29,2020D8 and 7653RD were obtained.利用sacB基因对蔗糖的敏感性进行标记质粒的消除,获得2020和7653R的质粒消除突变株2020D29,2020D8和7653RD。
5)plasmid elimination质粒消除
1.The plasmid elimination methods that were applied in probing plasmid function of Mesorhizobium huakuii were introduced in this paper.结合对华癸中生根瘤菌(Mesorhizobium huakuii)质粒功能的研究,介绍了高温和吖啶橙处理法、标记质粒消除法和质粒的不相容性消除质粒法在华癸中生根瘤菌质粒消除中的应用,并比较了各方法的优缺点。
2.High temperature and the elimination agent were combined by plasmid elimination for a cadmium-resistant Lactococcus lactis strains,the amount and the time by using temperature and eliminaton agent acridine orange (AO) were approached on the effect of cadmium-resistant Lactococcus lactis plasmid.质粒消除是鉴定质粒和获得无质粒菌株的重要方法,是乳酸菌进行遗传学改造所需的一项重要技术。
3.Ever since the conception of plasmid elimination was mentioned,more and more researches have been carried out and acquired some advancement.质粒消除剂是指通过抑制质粒的拷贝或复制使子代细菌中的质粒消失,并使其介导的耐药性也随之丧失的一些物质。
6)plasmid elimination质粒排除