1)protein splicing蛋白质剪接
1.everal cases of protein splicing have beenfound recently which is different from so calledRNA splicing.最近在几种生物体中连续发现了蛋白质剪接现象，由于它与通常所说的ＲＮＡ剪接的区别，人们称这些在蛋白质水平被剪切掉的部分为“蛋白质内含子”。
2.This process is termed as protein splicing.这种翻译后的加工过程称为蛋白质剪接(protein splicing)。
3.Autocatalytic protein splicing mediated by intein is carried out with 100% efficiency in primitive species.这种含有可选择蛋白质外显子的筛选系统,将蛋白质剪接与卡那霉素抗性相结合,直接从平板上观测剪接结果,成为快速、稳定筛选在异源物种中具有剪接活性蛋白内含子的新手段。
英文短句/例句
1.protein splicing蛋白质剪接[通过翻译后加工去除蛋白质内含子]
2.A New Protein Trans-splicing System of PRP8 Split InteinPRP8蛋白质反式剪接系统的建立
3.protein isoform蛋白质异形体[例如由同种mrna前体经可变剪接而产生的不同蛋白质]
4.Improved Protein Splicing by Directed Evolution of Inteins in Heterogenous Host异源宿主中蛋白质内含子剪接活性的定向进化
5.The Impact of SR Proteins and hnRNPA1 Splicing Factor Regulating the Alternative Splicing of Human Glucocorticoid Receptor Exon 9;剪接因子SR蛋白和hnRNPA1对糖皮质激素受体外显子9选择性剪接调控的影响
6.Directed Evolution, Cleavage, Segregate Expression of Inteins in Vivo and Splicing in Vitro蛋白质内含子体内定向进化和切割、分段表达及体外剪接
7.Study on the Function of Adapter Protein NRBP and a Novel Isoform of Caspase-10;接头蛋白NRBP及蛋白酶caspase-10新剪切体的功能研究
8.Study of Relationship between Long Splice Variant N Type Calcium Channel and CASK;神经突触蛋白CASK与钙通道长剪接变体关系研究
9.Expression and Regulation of Pre-mRNA Splicing Factor ZNF265 and Its Function;前体mRNA剪接蛋白ZNF265的表达、调控及功能研究
10.Large Protein Production Through Split Intein-Mediated Trans-Splicing通过断裂内含肽介导的反式剪接合成大的蛋白
11.Intein-mediated Trans-splicing of Chloride Ion Channel CFTR内含肽介导的氯离子通道蛋白CFTR的反式剪接
12.The alternative splicing & expression of fibronectin Ⅲ_(CS) segment and its relationship with wound healing;纤维连接蛋白Ⅲ_(CS)片段的剪接、表达以及与创伤修复的关系
13.The expression of splicing fibronectin variants of cutaneous injuries in rats;大鼠皮肤损伤后纤维连接蛋白剪接异型体的表达
14.The research progression of fibronectin EDA s expression and functions;纤维连接蛋白EDA片段的剪接表达及其功能研究进展
15.Investigation on Function of the Novel Protein Encoded by the 2.2Kb Spliced Variant of Hepatitis B Virus Genome;2.2kb乙型肝炎病毒基因组剪接变异体特异性新蛋白的功能研究
16.Characterization of Molecular Mechanism of a Transcriptional Coactivator-human p100 Protein in Pre-mRNA Splicing.;转录激活因子—人类p100蛋白参与pre-mRNA剪接加工分子机制研究
17.Expression and Location of Two Survivin Splice Variants and Their Interaction in HeLa Cells两种存活蛋白变异剪接体在HeLa细胞中的表达定位及相互作用
18.Effects of the splicing-specific novel protein encoded by 458 nt-1308 nt spliced variants of the hepatitis B virus genome on the gene expression profile in Huh7 hepatocytes458nt～1308nt乙型肝炎病毒剪接特异性蛋白对Huh7细胞基因表达的影响
相关短句/例句
protein trans-splicing蛋白质反式剪接
1.In this study we explored the intein-mediated protein trans-splicing to deliver a Phe309→Ser mutant full-length fVⅢ (F309SfVⅢ) gene by using a dual-vector system.本文旨在利用内含肽(intein)的蛋白质反式剪接,研究双载体真核细胞转具有促分泌作用的F309S突变体全长fVⅢ(F309SfVⅢ)基因。
2.[Objective] By exploring Ssp DnaE intein-catalyzed protein trans-splicing we investigated the ligation of expression product of ATP-binding cassette transporter A1(ABCA1)gene in E.【目的】利用SspDnaEintein的蛋白质反式剪接技术研究在大肠杆菌中对ABCA1基因表达产物的连接作用。
3.We studied the ligation of coagulation factor VIII heavy and light chains in Escherichia coli by utilizing the intein-mediated protein trans-splicing.研究利用intein的蛋白质反式剪接功能在大肠杆菌中对凝血VIII因子(FVIII)重链和轻链的连接作用,将B结构域大部分缺失型FVIII(BDD-FVIII)于满足剪接所需的保守性氨基酸Ser1657前断裂为重链和轻链,分别与split mini Ssp DnaB intein的106个氨基酸的N端(Int-N)和48个氨基酸的C端(Int-C)融合,构建到原核表达载体pBV220。
3)protein splicing蛋白剪接
1.We want to test whether protein splicing can occur in higher eucaryotic cell,which would play an important role in transgene containment in transgenic plants.将 4种重组质粒中的aroA In(蛋白内含子Intein插入aroA)融合基因插入 pET 32得到表达载体 pETLEBC、pETLEBT、pETLERC和 pETLERT ,IPTG诱导后 ,SDS PAGE分析显示其能在DE3 中有效表达并发生相应的蛋白剪接。
4)protein trans-splicing system蛋白质反式剪接系统
1.Therefore a new protein trans-splicing system using an artificial PRP8 split intein from Cryptococcus neoformans serotype AD was successfully constructed and was shown to be efficient in ligation of flanking proteins.利用此Cryptococcus neoformans AD血清型PRP8断裂蛋白质内含子,成功构建了蛋白质反式剪接系统。
5)pre-mRNA splicing protein前体mRNA剪接蛋白
6)Protein ligation蛋白质连接
延伸阅读
剪接分子式：CAS号：性质：又称拼接，粘接，缝接。(1)在基因操作中，酶催化使DNA裂片接合起来；(2)在切除内含子(introns)后，把保留的mRNA连接起来。
